CN103923831B - For extracting the instruments and methods of biologically active substance - Google Patents
For extracting the instruments and methods of biologically active substance Download PDFInfo
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- CN103923831B CN103923831B CN201310014908.3A CN201310014908A CN103923831B CN 103923831 B CN103923831 B CN 103923831B CN 201310014908 A CN201310014908 A CN 201310014908A CN 103923831 B CN103923831 B CN 103923831B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The invention provides a kind of self-reacting device of efficient extraction biologically active substance, it comprises: shell, base plate, worktable, needle and needle travel mechanism, sleeve pipe and cannula translating mechanism and vibrating mechanism, described vibrating mechanism can produce vibration and make cannula translating mechanism carry out up-down vibration, wherein, needle can vertically insert and depart from sleeve pipe, and sleeve pipe vertically can insert and depart from sample preparation district, vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, it is arranged so that anyway the amplitude size vibrated changes, and the lower-most point of its amplitude is constant.Present invention also offers the method using described instrument Isolation and purification biologically active substance particularly nucleic acid.Instrument of the present invention can adjust arbitrarily vibrational frequency and amplitude, and increases substantially the setting range of vibrational frequency, has low noise, the advantage of high life.
Description
Technical field
The present invention relates to biology and apparatus field.Concrete, the invention provides a kind of self-reacting device extracting biologically active substance, and use described instrument to extract the method for biologically active substance.
Background technology
Biologically active substance is extracted at multiple fields such as modern clinic medical diagnosis on disease, transfusion safety, Forensic Identification, environmental microorganism detection, food safety detection, molecular biology researches, such as cell, and the biologically active substance such as protein, nucleic acid has vital role.Wherein, the biologically active substance compound that nucleic acid is become by many nucleotide polymerization is one of the most base substance of life.Nucleic acid is extensively present in all animals, vegetable cell, microorganism, in organism.Different according to chemical constitution, nucleic acid can be divided into Yeast Nucleic Acid, is called for short RNA and thymus nucleic acid, is called for short DNA.DNA is the essential substance basis storing, copy and convey hereditary information, and RNA plays an important role in protein building-up process.The development of biotechnology is maked rapid progress, along with round pcr is in the application of every field, comprise medical science aspect disease detection, agriculture aspect detection GMOs and other very many-sided application etc., need high-throughout sample biologically active substance to extract and the method for full-automatic biologically active substance extraction and instrument.
Utilize paramagnetic particle method extract biologically active substance particularly active macromolecules be the technology that developed recently gets up.Paramagnetic particle method is that utilize can the magnetic bead of specific recognition cell, protein or nucleic acid, this magnetic bead can be combined specifically with nucleic acid molecule, then under the influence of a magnetic field, separate from the active macromolecules blood, animal tissues, food, pathogenic micro-organism equal samples.Such as, the paramagnetic particle method of nucleic acid is extracted and according to the principle identical with pellosil centrifugal column, the surface of nanotechnology to superparamagnetic nano particle can be used to modify, be prepared into superparamagnetism monox nanometer magnetic bead.This magnetic bead can identify specifically with nucleic acid molecule.Utilize the superparamagnetism of monox nanometer microballoon, under the effect of Chaotropic salt (Guanidinium hydrochloride, guanidinium isothiocyanate etc.) and externally-applied magnetic field, from sample, DNA and RNA can be separated.
Paramagnetic particle method automatic extracting instrument is divided into again bar magnet method and suction method.Suction method is undertaken by automatic pipetting device.For paramagnetic particle method instrument for extracting nucleic acid, add lysate, magnetic bead absorption by automatic pipetting device; Take solution away, add rinsing liquid, magnetic bead adsorbs, and takes rinsing liquid away, adds elution buffer.Owing to not taking magnetic bead away time suction method exists and removes waste liquid, pipettor can not lean on magnetic bead too near, in case magnetic bead together with waste liquid by the problem taken out, affect elution efficiency and purity.
It is the technology that developed recently gets up that bar magnet method is extracted, and its principle is that magnetic separating device is immersed liquid, makes the particle that can be attracted magnetically in mixture concentrate on separating device surface by the action of a magnetic field.Wherein in a kind of mode, magnetic separating device is a sleeve pipe, makes sleeve pipe produce magnetic by inserting bar magnet, attract the granular absorption that can be attracted magnetically in mixture to sleeve surface, when bar magnet is detached, sleeve pipe loses magnetism, and the particle be attracted magnetically departs from sleeve pipe.The flux of bar magnet method instrument for extracting nucleic acid has 8,16,32,96 passages usually, and relative to suction method, the advantage of bar magnet method is that the liquid that each walks does not remain, because bar magnet only takes away magnetic bead, transfers in the corresponding reacting hole of next step.Drive the mechanism of bar magnet that bar magnet can be driven to move up and down in sleeve pipe in bar magnet method instrument, contribute to the cracking of leaching process and rinsing and purifying.
Summary of the invention
The invention provides a kind of self-reacting device utilizing bar magnet method to extract biologically active substance efficiently, can be applicable to the multiple fields such as clinical disease diagnosis, transfusion safety, Forensic Identification, environmental microorganism detection, food safety detection, molecular biology research, for Isolation and purification biologically active substance, such as cell, albumen and nucleic acid.
Concrete, the invention provides a kind of instrument for extracting biologically active substance, comprising:
Shell and base plate 1,
Worktable 15, it has sample preparation district,
Needle 7 and needle travel mechanism 48, needle travel mechanism comprises needle support 8 and needle lifting mechanism 33, and needle is removably fixed on needle support, wherein, by moving up and down of needle lifting mechanism, makes needle vertically to insert and to depart from sleeve pipe,
Sleeve pipe 4 and cannula translating mechanism 16, cannula translating mechanism comprises casing support 6 and telescopic lifter structure 18, sleeve pipe is removably fixed on casing support, wherein, by moving up and down of telescopic lifter structure, make sleeve pipe vertically can insert and depart from sample preparation district, in addition, cannula translating mechanism 16 can up-down vibration;
For supporting the mechanism rack 13 of described needle travel mechanism 48 and cannula translating mechanism 16,
With
Vibrating mechanism 19, described vibrating mechanism can produce and export vibration, makes cannula translating mechanism 16 carry out up-down vibration, wherein,
Vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, and wherein said rotating mechanism has wheel disc and axle center, and when described rotating mechanism rotates around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thus produces vibration.An aspect wherein, the feature of instrument of the present invention is, described lever arm, movable fulcrum and rotating mechanism be arranged so that anyway the amplitude size of the vibration produced changes, and its lower-most point exporting to the amplitude of the vibration of cannula translating mechanism is constant.Thus, how the amplitude of the vibration no matter vibrating mechanism produces and exports changes, and also namely no matter how its conduction changes to the amplitude of casing support, and the lower-most point of the amplitude of the pipe box of instrument of the present invention is constant.
Rotating mechanism in the vibrating mechanism of instrument of the present invention has wheel disc and the axle center of ad hoc type line profile, and when these rotatable parts rotate around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, produces and exports vibration.Of the present invention in one of them, described rotating mechanism is eccentric rotary mechanism, and the Bu center, axle center of its rotatable parts, is eccentric rotary mechanism.Eccentric rotary mechanism can be eccentric wheel etc.
In the present invention, the some profile outer rim of described rotating mechanism (particularly eccentric rotary mechanism) being formed into the shortest distance in its axle center is called nearest profile point.In the present invention, the working order of vibrating mechanism refers to that the rotating mechanism of vibrating mechanism rotates around axle center, and the outer rim of its wheel disc promotes state when lever arm rotates around balance pivot.In the present invention, when the nearest profile point of rotating mechanism described in vibrating mechanism produces pushing effect (being generally nearest profile point and the lever arm contacts of rotating mechanism) to lever arm, in vibrating mechanism, lever arm rotates around balance pivot, and lever arm now can be supplied to be called lowest amplitude position of the fulcrum around the position of rotating lever fulcrum.In one embodiment of the present invention, in instrument of the present invention, the lever arm of vibrating mechanism, being set to of movable fulcrum and rotating mechanism, in working order, the change of the position of movable fulcrum is limited to lowest amplitude position of the fulcrum.Thus, how the displacement that the rotating mechanism of no matter vibrating mechanism can be made to promote lever arm changes, also no matter how the position of movable fulcrum on lever arm adjusts, also how the amplitude of the i.e. vibration no matter vibrating mechanism produces and exports changes, the lower-most point of the amplitude of its vibration exported is all constant, thus how the amplitude making no matter vibrating mechanism export to casing support changes, the lower-most point of its amplitude is constant, also namely makes the lower-most point of the amplitude of the pipe box of instrument of the present invention constant.In instrument of the present invention, vibrating mechanism is by a fixing some output amplitude on lever arm, and this point is normally relatively rotating the opposite side of fulcrum of mechanism and lever arm contacts point.How the amplitude no matter vibrating mechanism exports to casing support changes, constant in the lower-most point of the amplitude of this point of fixity, also namely makes the lower-most point of the amplitude of the pipe box of instrument of the present invention constant.
The rotating mechanism used in the present invention can be eccentric rotary mechanism, and it has the wheel disc of axle center (i.e. turning point) and ad hoc type line profile, Bu center, its axle center.Preferably, described rotating mechanism can adopt circular as profile, is eccentric wheel.Adopt circular wheel to have easily manufactured, technique is simple, and the amplitude exporting vibration is easy to the advantages such as adjustment.In addition, the cam with non-central axle center also can as eccentric rotary mechanism of the present invention.
Have central hub but also can be used for the present invention by vibrative rotating mechanism, such as outline is oval cam.
In the present invention, movable fulcrum and the way of contact of lever arm can be that fulcrum contacts or embed the groove or hollow groove etc. that are arranged on lever arm with the lower edge of lever arm.
In one embodiment of the invention, movable fulcrum is multiple fulcrums that can independently be separated with lever arm or contact, and comes in contact respectively as required and forms single fulcrum, can produce the vibration with particular amplitude thus with lever arm.Such as, can below lever arm or side arrange multiple can lifting or the fulcrum block moved horizontally or fulcrum shaft, each fulcrum block or fulcrum shaft can contact with lever arm lower edge in lifting, or mobilely embed lever arm lower edge or groove, independently form fulcrum.
In another embodiment of the present invention, movable fulcrum is set to along the mobile fulcrum of the lower edge of lever arm or the groove be arranged on lever arm or hollow groove movement, can to produce the vibration of the amplitude with continuous variable thus.Such as, movable fulcrum is mounted in the fulcrum shaft on Mobile base, and fulcrum shaft contacts with the lower edge of lever arm, or the groove inserted on lever arm or hollow groove, under the driving of Mobile base, move formation fulcrum along lever arm.
Lever arm is preferably set to linear lever arm.Described lever arm can have the groove parallel with its upper surface or hollow groove, may be used for for a point cantact.Mobile fulcrum can move along the edge of lever arm (lower edge) or the groove be located on lever arm or hollow groove, and its travel direction is the axial direction due of lever arm.In this case, to be set to the axle center of eccentric rotary mechanism equal to the distance of lever arm with described axle center to the minor increment of profile outer rim for vibrating mechanism.When eccentric rotary mechanism is eccentric wheel, it is equal to the distance of lever arm with its axle center that vibrating mechanism is set to eccentric minimum radius (namely axle center is to the minor increment of wheel rim).In this case, the radial direction of eccentric wheel minimum radius is vertical with the travel direction (i.e. the axis of lever arm) that lever moves fulcrum.
Lever arm also can be the lever arm of broken-line type or shaped form.
Of the present invention wherein in another, mobile fulcrum comprises traversing carriage 23, traversing carriage driving mechanism 24 and mobile fulcrum shaft 37, lever arm has the hollow groove parallel with its upper surface, mobile fulcrum shaft is through hollow groove, move in hollow groove under traversing carriage driving mechanism drives, form the mobile fulcrum of lever.
In one aspect of the invention, lever arm has vibrating wheels 25, and described vibrating wheels contacts with telescopic lifter structure, conduction vibration, and described vibrating wheels and rotating mechanism lay respectively at the both sides of mobile fulcrum.
In one aspect of the invention, in the instrument of the invention described above, needle support 8 comprises needle support end block 9 and needle supported arm 34, and needle is removably fixed on needle supported arm; Described needle lifting mechanism comprises the needle be arranged on below needle support end block and promotes end block 17, when needle promotion end block moves upward, promote needle support end block and move upward, thus promotion needle support moves upward.Of the present invention in another, in above-mentioned instrument, needle promotes end block 17 with needle support 8 is separable, when needle support contacts with casing support in the process declined, needle promotes end block and is separated with needle support, and needle support is supported by casing support and together moves with casing support.
In one aspect of the invention, in the instrument of the invention described above, casing support 6 comprises casing support end block 35 and casing support arm 5, and sleeve pipe is removably fixed on casing support arm; Described telescopic lifter structure comprises the sleeve pipe lifting end block 36 be arranged on below casing support end block, when sleeve pipe lifting end block moves upward, pushing set tube support end block moves upward, thus pushing set tube support moves upward, lifting end block is when moving downward, and casing support end block is elevated end block according to action of gravity and sleeve pipe and synchronously moves downward.Of the present invention in another, the lifting end block of sleeve pipe described in above-mentioned instrument and casing support are separable.In addition, in one embodiment of the invention, arrange cover tube vibration push rod 26 below the casing support end block of above-mentioned instrument, it is connected with vibrating mechanism 19, conduction vibration.
In one aspect of the invention, in the instrument of the invention described above, casing support has at least one casing support arm, and preferably, casing support has two casing support arms, and supported arm has can the bushing slot of fixed sleeving.
In one aspect of the invention, in the instrument of the invention described above, needle support has at least one needle supported arm, and preferably, needle support has two supported arms, supported arm has the needle groove can fixing needle.
In one aspect of the invention, in the instrument of the invention described above, worktable can move horizontally.Wherein sample preparation district can comprise workspace, sample cell or sample well, or its combination.Of the present invention in another, described sample preparation district has bottoming hole, configures well heater below it.
In one aspect of the invention, described in the instrument of the invention described above, needle is permanent magnet material.
In one aspect of the invention, described in the instrument of the invention described above, sleeve pipe is nonmagnetic substance.
The instrument of the invention described above may be used for extracting biologically active substance, such as cell, protein or nucleic acid.
The instrument of the invention described above can be the instrument for Isolation and purification nucleic acid.
Present invention also offers a kind of method using above-mentioned instrument Isolation and purification biologically active substance.
In another aspect of the present invention, provide a kind of for adopting the vibrating mechanism of the biologically active substance extraction apparatus of bar magnet method, described extraction apparatus has needle and needle travel mechanism, and sleeve pipe and cannula translating mechanism, by moving up and down of needle travel mechanism, needle is made vertically to insert and to depart from sleeve pipe, moving up and down by cannula translating mechanism, sleeve pipe is made can vertically to insert and depart from sample preparation district below it, in addition, described vibrating mechanism can produce vibration, drive the sleeve pipe up-down vibration of described extraction apparatus, it is characterized in that, described vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, wherein said rotating mechanism has wheel disc and axle center, when described rotating mechanism rotates around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thus produce vibration.In the present invention, the lever arm of above-mentioned vibrating mechanism, movable fulcrum and rotating mechanism be arranged so that anyway the amplitude size of the vibration produced changes, and its lower-most point exporting to the amplitude of the vibration of cannula translating mechanism is constant.
Rotating mechanism in vibrating mechanism of the present invention has wheel disc and the axle center of ad hoc type line profile, and when these rotatable parts rotate around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, produces and exports vibration.Of the present invention in one of them, described rotating mechanism is eccentric rotary mechanism, and the Bu center, axle center of its rotatable parts, is eccentric rotary mechanism.Eccentric rotary mechanism can be eccentric wheel etc.
An aspect wherein, the lever arm of vibrating mechanism of the present invention, being set to of movable fulcrum and rotating mechanism, in working order, the change of the position of movable fulcrum is limited to lowest amplitude position of the fulcrum.Thus, how the displacement that the rotating mechanism of no matter vibrating mechanism can be made to promote lever arm changes, also no matter how the position of movable fulcrum on lever arm adjusts, also how the amplitude of the i.e. vibration no matter vibrating mechanism produces and exports changes, the lower-most point of amplitude described in it is all constant, thus how the amplitude making no matter vibrating mechanism export to the casing support of biologically active substance extraction apparatus changes, the lower-most point of its amplitude is constant, also namely makes the lower-most point of the amplitude of the pipe box of instrument of the present invention constant.Usually, vibrating mechanism is by a fixing some output amplitude on lever arm, and this point is normally relatively rotating the opposite side of fulcrum of mechanism and lever arm contacts point.How the amplitude no matter vibrating mechanism exports to casing support changes, constant in the lower-most point of the amplitude of this point of fixity, also namely makes the lower-most point of the amplitude of the pipe box of instrument of the present invention constant.
The rotating mechanism used in vibrating mechanism of the present invention can be eccentric rotary mechanism, and it has the wheel disc of axle center (i.e. turning point) and ad hoc type line profile, Bu center, its axle center.Preferably, described rotating mechanism can adopt circular as profile, is eccentric wheel.Adopt circular wheel to have easily manufactured, technique is simple, and the amplitude exporting vibration is easy to the advantages such as adjustment.In addition, the cam with non-central axle center also can as eccentric rotary mechanism of the present invention.
Have central hub but can vibrative rotating mechanism also vibrating mechanism used in the present invention, such as outline is oval cam.
In vibrating mechanism of the present invention, movable fulcrum and the way of contact of lever arm can be that fulcrum contacts or embed the groove or hollow groove etc. that are arranged on lever arm with the lower edge of lever arm.
In a kind of embodiment of vibrating mechanism of the present invention, movable fulcrum is multiple fulcrums that can independently be separated with lever arm or contact, and comes in contact respectively as required and forms single fulcrum, can produce the vibration with particular amplitude thus with lever arm.Such as, can below lever arm or side arrange multiple can lifting or the fulcrum block moved horizontally or fulcrum shaft, each fulcrum block or fulcrum shaft can contact with lever arm lower edge in lifting, or mobilely embed lever arm lower edge or groove, independently form fulcrum.
In another embodiment of vibrating mechanism of the present invention, movable fulcrum is set to along the mobile fulcrum of the lower edge of lever arm or the groove be arranged on lever arm or hollow groove movement, can to produce the vibration of the amplitude with continuous variable thus.Such as, movable fulcrum is mounted in the fulcrum shaft on Mobile base, and fulcrum shaft contacts with the lower edge of lever arm, or the groove inserted on lever arm or hollow groove, under the driving of Mobile base, move formation fulcrum along lever arm.
In vibrating mechanism of the present invention, lever arm is preferably set to linear lever arm.Described lever arm can have the groove parallel with its upper surface or hollow groove, may be used for for a point cantact.Mobile fulcrum can move along the edge of lever arm (lower edge) or the groove be located on lever arm or hollow groove, and its travel direction is the axial direction due of lever arm.In this case, to be set to the axle center of eccentric rotary mechanism equal to the distance of lever arm with described axle center to the minor increment of profile outer rim for vibrating mechanism.When eccentric rotary mechanism is eccentric wheel, it is equal to the distance of lever arm with its axle center that vibrating mechanism is set to eccentric minimum radius (namely axle center is to the minor increment of wheel rim).In this case, the radial direction of eccentric wheel minimum radius is vertical with the travel direction (i.e. the axis of lever arm) that lever moves fulcrum.
In vibrating mechanism of the present invention, lever arm also can be the lever arm of broken-line type or shaped form.
In vibrating mechanism of the present invention in another, mobile fulcrum comprises traversing carriage 23, traversing carriage driving mechanism 24 and mobile fulcrum shaft 37, lever arm has the hollow groove parallel with its upper surface, mobile fulcrum shaft is through hollow groove, move in hollow groove under traversing carriage driving mechanism drives, form the mobile fulcrum of lever.
In of vibrating mechanism of the present invention, lever arm has vibrating wheels 25, and described vibrating wheels contacts with telescopic lifter structure, conduction vibration, and described vibrating wheels and rotating mechanism lay respectively at the both sides of mobile fulcrum.
Present invention also offers and a kind ofly use the vibrating mechanism of the invention described above in bar magnet method biologically active substance extraction apparatus to the vibrative method of the sleeve pipe of extraction apparatus.
Accompanying drawing explanation
By reading the detailed description done non-limiting example done with reference to the following drawings, other features, objects and advantages of the present invention will become more obvious:
Fig. 1 is first direction stereographic map in accordance with a preferred embodiment of the present invention;
Fig. 2 is second direction stereographic map in accordance with a preferred embodiment of the present invention;
Fig. 3 is side cross sectional views in accordance with a preferred embodiment of the present invention;
Fig. 4 is side cross sectional views in accordance with a preferred embodiment of the present invention;
Fig. 5 is sectional block diagram in accordance with a preferred embodiment of the present invention.
Reference numeral
1 base plate
2 heated sample grooves
3 non-heated workspace
4 sleeve pipes
5 casing support arms
6 casing supports
7 needles
8 needle supports
9 needle support end blocks
10 optical axises
11 motors one
12 motors two
13 mechanism racks
14 mechanism's platforms
15 traversers
16 cannula translating mechanism
17 needles promote end block
18 telescopic lifter structures
19 vibrating mechanisms
20 eccentric wheel drive-motor
21 eccentric wheels
22 lever arms
23 traversing carriages
24 traversing carriage driving mechanisms
25 vibrating wheels
26 cover tube vibration push rods
31 screw mandrels one
32 screw mandrels two
33 needle lifting mechanisms
34 needle supported arms
35 casing support end blocks
36 sleeve pipe lifting end blocks
37 move fulcrum shaft
48 needle travel mechanisms
Embodiment
For a better understanding and interpretation of the present invention, the present invention is described in further detail below with reference to accompanying drawings.
Fig. 1 is the first direction stereographic map of the inside of the instrument for extracting biologically active substance according to a preferred embodiment of the present invention.Instrument of the present invention can be used for Isolation and purification nucleic acid.This instrument has shell (not illustrating at accompanying drawing) and base plate 1, needle 7 and needle travel mechanism 48, sleeve pipe 4 and cannula translating mechanism 16, for supporting the support 13 of described needle travel mechanism and cannula translating mechanism, and for generation of vibration, make cannula translating mechanism carry out the vibrating mechanism 19 of up-down vibration.
Shell (not illustrating at accompanying drawing) can by instrument internal and air spaced apart, in order to avoid sample contamination and damage parts.Housing face-plate is generally metallic substance and makes.Shell can have the sample window that can open and close, for putting into and taking out sample.Shell can have the viewing window made with transparent material.Sample window and viewing window can be same parts.Shell can have the external apparatus interface be connected with peripheral equipment in addition, the display screen of operation display interface, various manipulation of switches, button or button etc.
Sleeve pipe is the pipe with closed bottom and hollow tube chamber.Sleeve pipe can have such as elongated cylinder form.The material of sleeve pipe is normally nonmagnetic.The Choice and design of its material and thickness is for not weakened field or only affect magnetic field a little, but needs the magnetic field that ensures to produce the biological sample that can attract magnetic bead or band magnetic bead outward at sleeve pipe when inserting needle.
Needle can vertically insert and depart from sleeve pipe.The bottom (namely inserting one end of sleeve pipe) of needle has magnetic.When needle insert with contact or close to sleeve bottom time, magnetic field can be produced outward at sleeve pipe, thus realize sleeve pipe to the magnetic bead in the outer solution of sleeve pipe or the absorption of biological sample and the disengaging of being with magnetic bead.In the present embodiment, the bottom of needle has permanent magnet, and band is magnetic thus.In other embodiments of the present invention, needle produces magnetic (electromagnet) by electromagnetic action.
Fig. 2 is the second direction stereographic map of the above-mentioned instrument for extracting biologically active substance according to a preferred embodiment of the invention.
As depicted in figs. 1 and 2, the described instrument for extracting biologically active substance has the worktable 15 that can move horizontally.As shown in Figure 2, the worktable 15 that can move horizontally has sample preparation district.Sample preparation district can comprise workspace, sample cell, sample well, or its combination.Sample preparation district can the sample cell of processing sample or sample well for arranging, or for placing the vessel of load sample, as type culture plates such as single or single centrifuge tube, 24 orifice plates or 96 orifice plates, also can put the non-standard culture plate of needs.
In illustrated the preferred embodiments of the present invention instrument, worktable has Liang Zu sample preparation district with corresponding two groups of needles and cannula system.Mei Zu sample preparation district comprises the non-heated workspace 3 in two groups of heated sample grooves 2 and these two groups of sample cells respectively.Heated sample groove can insert the containers such as single or single centrifuge tube.Non-heated workspace can put the containers such as single or single centrifuge tube, also can put the type culture plate such as 24 orifice plates or 96 orifice plates, can also put the non-standard culture plate of needs.Worktable 15 is by the driving mechanisms control be arranged at below it, can move in the horizontal direction, make sample preparation district move to the below of sleeve pipe, sleeve pipe vertically can insert and depart from the sample cell in sample preparation district, or be fixed on test tube or the EP pipe of sample cell, or the hole of culture plate.
The described instrument for extracting biologically active substance has needle 7 and needle travel mechanism 48.Needle travel mechanism 48 comprises needle support 8 and needle lifting mechanism 33.
Needle support 8 comprises needle support end block 9 and needle supported arm 34.Needle is removably fixed on needle supported arm.Diagram the preferred embodiments of the present invention for extracting in the instrument of biologically active substance, needle support has two needle supported arms be arranged in parallel.Needle support also can only have a needle supported arm, also can be above two.The quantity of the fixable needle of each needle supported arm can adjust as required, and often group is 1-12 root needle usually, and common is 4,6 or 12 needles.In another embodiment, needle supported arm can fix needle group, and each needle group has arranges needle more, 24 needles (spread pattern is 4 × 6) of such as corresponding culture plate, 96 needles (spread pattern is 8 × 12) etc.In the present embodiment, the bottom of needle has permanent magnet, and band is magnetic thus.In other embodiments of the present invention, needle also produces magnetic by electromagnetic action.
Needle lifting mechanism comprises needle and promotes end block 17, motor 12, leading screw 2 32 and nut transmission pair, and motor drives promotion end block 17 to move upward by leading screw and nut transmission pair.Needle promotes end block 17 and is arranged on below needle support end block 9.Needle promotes end block and can contact with needle support end block.When motor by leading screw and nut transmission pair drive promote end block move upward time, needle promotes end block and contacts with needle support end block, and promotes needle support end block and move upward, thus promotion needle support moves upward.
The described instrument for extracting biologically active substance also has sleeve pipe 4 and cannula translating mechanism 16.Cannula translating mechanism comprises casing support 6 and telescopic lifter structure 18.Casing support comprises casing support end block 35 and casing support arm 5.Diagram the preferred embodiments of the present invention for extracting in the instrument of biologically active substance, casing support has two casing support arms be arranged in parallel.Casing support also can only have a casing support arm, also can be above two.Sleeve pipe is removably fixed on casing support arm.The quantity of the fixable sleeve pipe of each casing support arm can adjust as required.The quantity of sleeve pipe can adjust as required, and often group is 1-12 root sleeve pipe usually.Common is 4,6 or 12 sleeve pipes.In another embodiment, casing support arm can fixed sleeving group, and each sleeve pipe group has many bar casing tubes, 24 sleeve pipes (spread pattern is 4 × 6) of such as corresponding culture plate, 96 sleeve pipes (spread pattern is 8 × 12) etc.
Telescopic lifter structure comprises sleeve pipe lifting end block 36, motor 11, leading screw 1 and nut transmission pair.Motor drives sleeve pipe lifting end block to move up or down by leading screw and nut transmission pair.Sleeve pipe lifting end block is arranged on below casing support end block.Sleeve pipe lifting end block can with the contact of casing support end block.When motor drives sleeve pipe lifting end block to move upward by leading screw and nut transmission pair, sleeve pipe lifting end block can contact with casing support end block, and pushing set tube support end block moves upward, thus pushing set tube support moves upward.When motor drives sleeve pipe lifting end block to move downward by leading screw and nut transmission pair, casing support end block is elevated end block according to action of gravity and sleeve pipe and synchronously moves downward, thus drive casing support moves downward.
Fig. 3 and Fig. 4 is described for extracting the side cross sectional views of the instrument of biologically active substance.As can be seen from Fig. 3 and Fig. 4, sleeve pipe lifting end block 36 is separable with casing support end block 35, realizes the vibration of sleeve pipe thus and moves up and down and can be manipulated by different institutions independently.
Sleeve pipe lifting end block is arranged at below casing support end block 35.When motor drives sleeve pipe lifting end block to move upward by leading screw and nut transmission pair, pushing set tube support moves upward.When motor drives sleeve pipe lifting end block to move downward by leading screw and nut transmission pair, casing support end block is elevated end block according to action of gravity and sleeve pipe and synchronously moves downward, thus drive casing support moves downward.
In diagram the preferred embodiments of the present invention for extracting in the instrument of biologically active substance, as shown in Figure 2 and Figure 5, arrange cover tube vibration push rod 26 below casing support end block, it is connected with vibrating mechanism, conduction vibration.
Diagram the preferred embodiments of the present invention for extracting in the instrument of biologically active substance, sleeve pipe lifting end block is arranged on below casing support end block, the length of its upper surface is identical with the lower surface of casing support end block with width, can be elevated end block play support and pushing effect to sleeve pipe.In the middle of sleeve pipe lifting end block, there is cavity.Cover tube vibration push rod is also arranged on below casing support end block, and its upper surface length is less than the lower surface of casing support end block with width.In the middle of sleeve pipe lifting end block, have cavity, cover tube vibration push rod can be allowed to pass, and the upper end of cover tube vibration push rod contacts with casing support end block, and lower end contacts with the vibrating mechanism be arranged on below cannula translating mechanism.
In one aspect of the invention, needle promotes end block 17 with needle support end block 9 is separable.Needle promotes a side contacts of end block and needle support end block.When motor drives promotion end block to move upward by leading screw and nut transmission pair, promote needle support by needle support end block and move upward.Needle can be made so upwards to depart from from sleeve pipe.Moving downward of needle support is according to action of gravity.The needle of instrument of the present invention promotes end block and needle support is separable, and this design can realize the abundant close contact of needle termination and sleeve bottom, simplifies the requirement to automatically controlled part, and the phenomenon preventing needle from impacting sleeve bottom occurs.
Fig. 3 illustrates the working order of described instrument when needle inserts sleeve pipe.Needle 4 inserts sleeve pipe, and its bottom contacts with the bottom of sleeve pipe.Now needle promotion end block 17 is separated with needle support end block 9, and needle support 8 is supported by casing support 6 and together moves with casing support.Another kind of embodiment is that needle 4 inserts sleeve pipe, arrives the position of the predetermined bottom closest to sleeve pipe, and now needle promotion end block 17 is separated with needle support end block 9, and needle support is supported by casing support and together moves with casing support.The position that predetermined needle bottom arrives closest to sleeve bottom can be limited by casing support 6 and arranging of needle support 8, such as be set to needle supported arm and casing support arm contact, or needle supported arm contacts with the male member that casing support arm is arranged, described male member can be cushioning material preparation.
Fig. 4 illustrates the working order that sleeve pipe is separated with needle.Needle promotes a side contacts of end block 17 and needle support end block 9.When motor drives needle promotion end block to move upward by leading screw and nut transmission pair, promote needle support by needle support end block and move upward, needle is separated with sleeve pipe.
In addition, sleeve pipe lifting end block 35 contacts with casing support end block 36, and when motor drives sleeve pipe lifting end block to move upward by leading screw and nut transmission pair, pushing set tube support moves upward.When motor drives sleeve pipe lifting end block to move downward by leading screw and nut transmission pair, casing support moves downward with sleeve pipe lifting end block under gravity.
Pass through when needing sleeve pipe to vibrate thus the biological sample of the magnetic bead that adsorbs outward of wash-out sleeve pipe or band magnetic bead time, needle support end block promotes needle support and moves upward, and sleeve pipe is separated with needle.Sleeve pipe lifting end block and casing support end block disengage.Casing support vibrates under vibrating mechanism effect.
Insert when needing sleeve pipe or the hole of the centrifuge tube of sample area or culture plate time, sleeve pipe lifting end block 35 contacts with casing support end block 36, when motor drives sleeve pipe lifting end block to move upward by leading screw and nut transmission pair, pushing set tube support moves upward and leaves sample area.When motor drives sleeve pipe lifting end block to move downward by leading screw and nut transmission pair, casing support moves downward with sleeve pipe lifting end block under gravity, inserts sample area.
In addition, as shown in the figure, described needle travel mechanism and cannula translating mechanism are arranged on mechanism's platform 14, are supported by mechanism rack 13.
The both sides of needle support end block 9, sleeve pipe lifting end block 36 and casing support end block 35 have perforation, article two, be vertically arranged on the perforation of the optical axis 10 on mechanism's platform through described needle support end block 9, sleeve pipe lifting end block 36 and casing support end block 35, restriction and fixed action are risen to needle support end block, sleeve pipe lifting end block and casing support end block.
The both sides that needle promotes end block also have perforation, are passed by another two optical axises.
Instrument for extracting biologically active substance of the present invention has vibrating mechanism, and described vibrating mechanism can produce vibration, makes cannula translating mechanism drive sleeve pipe to carry out up-down vibration.The sleeve pipe of the instrument of extraction biologically active substance of the present invention can carry out up-down vibration up to the frequency of 20Hz.Described vibrating mechanism of the present invention comprises lever arm, mobile fulcrum and eccentric stiffener, and described mobile fulcrum can move along lever arm.Instrument for extracting biologically active substance of the present invention in the course of the work, the upper and lower fast vibration of one of them procedure calls needle sheath.According to different job requirements, this vibration will have different amplitudes and frequency.The amplitude being arranged to vibrate and the frequency of the vibrating mechanism of the instrument for extracting biologically active substance of the present invention are adjustable; and anyway amplitude adjusts; the starting point (lower-most point) of its amplitude is unanimously identical; thus reach the object of the mechanisms such as protection sheath; together with further feature of the present invention; instrument of the present invention is made to have low noise, the advantage of high life.
As shown in Figure 5, driving mechanism of the present invention comprises linear lever arm 22, traversing carriage 23, mobile fulcrum shaft 37, drive the driving mechanism 24 that traversing carriage moves horizontally, contact and the vibrating wheels 25 of conduction vibration with the cover tube vibration push rod 26 of telescopic lifter structure, and eccentric wheel 21 and eccentric wheel drive-motor 20.Lever arm is linear pattern, has the hollow groove parallel with lower edge with its upper limb.Mobile fulcrum shaft, through hollow groove, can move in hollow groove along lever arm direction under traversing carriage driving mechanism drives, and forms the mobile fulcrum of lever.To contact with the cover tube vibration push rod 26 of telescopic lifter structure and the vibrating wheels 25 of conduction vibration and eccentric wheel lay respectively at the both sides of mobile fulcrum.Eccentric wheel and lever arm contacts, when eccentric wheel rotates, cause and be subjected to displacement at the lever arm (power arm) of the eccentric wheel side of fulcrum, the lever arm (resistance arm) of the opposite side of fulcrum and vibrating wheels then synchronously carry out rightabout displacement, and namely the rotation around fulcrum occurs lever arm.Eccentric rotation makes vibrating wheels vibrate, thus makes cannula translating mechanism drive sleeve pipe to carry out up-down vibration.Also, namely, in the vibrating mechanism of the instrument for extracting biologically active substance of the present invention, vibration is produced by eccentric stiffener (being eccentric wheel in embodiment), the speed of controlled frequency.The size of the controlled damping width of movement of the position of mobile fulcrum on lever arm, also can control the size of amplitude together with eccentric rotating manner.
In the instrument of the preferred embodiments of the present invention shown in Fig. 5, eccentric wheel directly contacts with linear pattern lever arm, eccentric minimum radius (namely axle center is to the minor increment of wheel rim) is equal to the distance of lever arm with its axle center, the travel direction (i.e. lever arm) that radial direction and the lever of eccentric wheel minimum radius move fulcrum is vertical, now, no matter how fulcrum moves in the hollow groove of lever arm, when the contact of eccentric wheel and lever arm generation minimum radius, vibrating wheels is all in the lower-most point of its amplitude.No matter how eccentric rotating speed changes, no matter also how the position of mobile fulcrum on lever arm changes, namely regardless of amplitude and the frequency of vibrating wheels, the lower-most point of vibrating wheels is all constant, and namely the lower-most point of vibration occurs sleeve pipe is constant.
In other embodiments of the present invention, also eccentric wheel can be replaced to realize object of the present invention with cam.
The instrument of extraction biologically active substance of the present invention is by adopting above-mentioned vibrating mechanism, vibrational frequency and amplitude can be adjusted arbitrarily, no matter how amplitude adjusts simultaneously, the minimum start position of various amplitude is all identical, the setting range of vibrational frequency can be increased substantially, whole instrument is made to have low noise, the advantage of high life.
Although describe in detail about example embodiment of the present invention and advantage thereof, being to be understood that when not departing from the protection domain of spirit of the present invention and claims restriction, various change, substitutions and modifications can being carried out to these embodiments.For other examples, those of ordinary skill in the art should easy understand maintenance scope in while, the order of processing step can change.
In addition, range of application of the present invention is not limited to the technique of the specific embodiment described in specification sheets, mechanism, manufacture, material composition, means, method and step.From disclosure of the present invention, to easily understand as those of ordinary skill in the art, for the technique existed at present or be about to develop, mechanism, manufacture, material composition, means, method or step later, wherein their perform the identical function of the corresponding embodiment cardinal principle that describes with the present invention or obtain the identical result of cardinal principle, can apply according to the present invention to them.Therefore, claims of the present invention are intended to these technique, mechanism, manufacture, material composition, means, method or step to be included in its protection domain.
Claims (41)
1., for extracting an instrument for biologically active substance, comprising:
Shell and base plate (1),
Worktable (15), it has sample preparation district,
Needle (7) and needle travel mechanism (48), needle travel mechanism comprises needle support (8) and needle lifting mechanism (33), needle is removably fixed on needle support, wherein, by moving up and down of needle lifting mechanism, needle is made vertically to insert and to depart from sleeve pipe
Sleeve pipe (4) and cannula translating mechanism (16), cannula translating mechanism comprises casing support (6) and telescopic lifter structure (18), sleeve pipe is removably fixed on casing support, wherein, by moving up and down of telescopic lifter structure, make sleeve pipe vertically can insert and depart from sample preparation district, in addition, cannula translating mechanism (16) can up-down vibration;
For supporting the mechanism rack (13) of described needle travel mechanism (48) and cannula translating mechanism (16),
With
Vibrating mechanism (19), described vibrating mechanism can produce and export vibration, makes cannula translating mechanism (16) carry out up-down vibration, wherein,
Vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, and wherein said rotating mechanism has wheel disc and axle center, and when described rotating mechanism rotates around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thus produces vibration.
2. instrument according to claim 1, wherein said lever arm, being set to of movable fulcrum and rotating mechanism, in working order, the change of the position of movable fulcrum is limited to lowest amplitude position of the fulcrum, lowest amplitude position of the fulcrum refers to, when the nearest profile point of the described rotating mechanism of vibrating mechanism produces pushing effect to lever arm, and the position of the balance pivot that lever arm rotates about it in vibrating mechanism.
3. the instrument described in claim 1 or 2, wherein said rotating mechanism is eccentric rotary mechanism, Bu center, its axle center.
4. instrument according to claim 3, wherein said eccentric rotary mechanism is eccentric wheel or the cam with non-central axle center.
5. the instrument described in claim 1 or 2, wherein said movable fulcrum is to move or at the mobile fulcrum of the groove be arranged on lever arm or hollow groove movement along the lower edge of lever arm.
6. the instrument of claim 1 or 2, wherein said movable fulcrum is multiple fulcrums that can independently be separated with lever arm or contact, and comes in contact respectively form single fulcrum with lever arm.
7. the instrument described in claim 1 or 2, wherein said lever arm is linear pattern lever arm.
8. instrument according to claim 7, wherein said lever arm is linear pattern lever arm, and it has the groove parallel with upper surface or hollow groove, for point cantact.
9. instrument according to claim 1, wherein said vibrating mechanism comprises linear pattern lever arm (22), mobile fulcrum and eccentric wheel (21), mobile fulcrum moves along lever arm, its travel direction is the axial direction due of lever arm, and wherein eccentric minimum radius is equal to the distance of lever arm with its axle center.
10. instrument according to claim 9, wherein move fulcrum and comprise traversing carriage (23), traversing carriage driving mechanism (24) and mobile fulcrum shaft (37), lever arm has the hollow groove parallel with its upper surface, mobile fulcrum shaft is through hollow groove, move in hollow groove under traversing carriage driving mechanism drives, form the mobile fulcrum of lever.
Instrument described in 11. claims 1 or 2, wherein lever arm has vibrating wheels (25), and described vibrating wheels contacts with telescopic lifter structure, conduction vibration, and described vibrating wheels and rotating mechanism lay respectively at the both sides of mobile fulcrum.
Instrument described in 12. claims 1 or 2, wherein needle support (8) comprises needle support end block (9) and needle supported arm (34), and needle is removably fixed on needle supported arm; Described needle lifting mechanism comprises the needle be arranged on below needle support end block and promotes end block (17), when needle promotion end block moves upward, promote needle support end block and move upward, thus promotion needle support moves upward.
13. instruments according to claim 12, wherein needle promotes end block (17) with needle support (8) is separable, when needle support contacts with casing support in the process declined, needle promotes end block and is separated with needle support, and needle support is supported by casing support and together moves with casing support.
Instrument described in 14. claims 1 or 2, wherein casing support (6) comprises casing support end block (35) and casing support arm (5), and sleeve pipe is removably fixed on casing support arm; Described telescopic lifter structure comprises sleeve pipe lifting end block (36) be arranged on below casing support end block, when sleeve pipe lifting end block moves upward, pushing set tube support end block moves upward, thus pushing set tube support moves upward, lifting end block is when moving downward, and casing support end block is elevated end block according to action of gravity and sleeve pipe and synchronously moves downward.
15. instruments according to claim 14, wherein said sleeve pipe lifting end block and casing support are separable.
16. instruments according to claim 15, wherein arrange cover tube vibration push rod (26) below casing support end block, it is connected with vibrating mechanism (19), conduction vibration.
Instrument described in 17. claims 1 or 2, wherein casing support has at least one casing support arm.
18. instruments according to claim 17, wherein casing support has two casing support arms, and supported arm has can the bushing slot of fixed sleeving.
Instrument described in 19. claims 1 or 2, wherein needle support has at least one needle supported arm.
20. instruments according to claim 19, wherein needle support has two supported arms, supported arm has the needle groove can fixing needle.
21. instruments according to claim 1, wherein worktable can move horizontally, and sample preparation district comprises workspace, sample cell or sample well.
22. instruments according to claim 1, wherein said sample preparation district has bottoming hole, configures well heater below it.
23. instruments according to claim 1, wherein said needle is permanent magnet material.
24. instruments according to claim 1, wherein said sleeve pipe is nonmagnetic substance.
25. instruments according to claim 1, wherein biologically active substance is cell, protein or nucleic acid.
26. instruments according to claim 1, it is the instrument for Isolation and purification nucleic acid.
27. 1 kinds of methods using the instrument Isolation and purification biologically active substance according to any one of claim 1-26.
28. 1 kinds of vibrating mechanisms for biologically active substance extraction apparatus, described extraction apparatus has needle and needle travel mechanism, and sleeve pipe and cannula translating mechanism, by moving up and down of needle travel mechanism, make needle vertically to insert and to depart from sleeve pipe, moving up and down by cannula translating mechanism, make sleeve pipe can vertically insert and depart from sample preparation district below it, in addition, described vibrating mechanism can produce vibration, drive described cannula translating mechanism up-down vibration
It is characterized in that, described vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, wherein said rotating mechanism has wheel disc and axle center, when described rotating mechanism rotates around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thus produces vibration.
29. vibrating mechanisms according to claim 28, wherein said lever arm, being set to of movable fulcrum and rotating mechanism, in working order, the change of the position of movable fulcrum is limited to lowest amplitude position of the fulcrum, lowest amplitude position of the fulcrum refers to, when the nearest profile point of the described rotating mechanism of vibrating mechanism produces pushing effect to lever arm, and the position of the balance pivot that lever arm rotates about it in vibrating mechanism.
Vibrating mechanism described in 30. claims 28 or 29, wherein said rotating mechanism is eccentric rotary mechanism, Bu center, its axle center.
31. vibrating mechanisms according to claim 30, wherein said eccentric rotary mechanism is eccentric wheel or the cam with non-central axle center.
Vibrating mechanism described in 32. claims 28 or 29, wherein said movable fulcrum is to move or at the mobile fulcrum of the groove be arranged on lever arm or hollow groove movement along the lower edge of lever arm.
The vibrating mechanism of 33. claims 28 or 29, wherein said movable fulcrum is multiple fulcrums that can independently be separated with lever arm or contact, and comes in contact respectively form single fulcrum with lever arm.
Vibrating mechanism described in 34. claims 28 or 29, wherein said lever arm is linear pattern lever arm.
35. vibrating mechanisms according to claim 34, wherein said lever arm is linear pattern lever arm, and it has the groove parallel with upper surface or hollow groove, may be used for and a point cantact.
36. vibrating mechanisms according to claim 28, wherein said vibrating mechanism comprises linear pattern lever arm (22), mobile fulcrum and eccentric wheel (21), mobile fulcrum moves along lever arm, its travel direction is the axial direction due of lever arm, and wherein eccentric minimum radius is equal to the distance of lever arm with its axle center.
37. vibrating mechanisms according to claim 28, wherein move fulcrum and comprise traversing carriage (23), traversing carriage driving mechanism (24) and mobile fulcrum shaft (37), lever arm has the hollow groove parallel with its upper surface, mobile fulcrum shaft is through hollow groove, move in hollow groove under traversing carriage driving mechanism drives, form the mobile fulcrum of lever.
Vibrating mechanism described in 38. claims 28 or 29, wherein lever arm has vibrating wheels (25), and described vibrating wheels contacts with telescopic lifter structure, conduction vibration, and described vibrating wheels and rotating mechanism lay respectively at the both sides of mobile fulcrum.
39. vibrating mechanisms according to claim 28, wherein said biologically active substance is cell, protein or nucleic acid.
40. vibrating mechanisms according to claim 28, wherein said extraction apparatus is the instrument of Isolation and purification nucleic acid.
41. 1 kinds use the vibrating mechanism according to any one of claim 28-40 in bar magnet method biologically active substance extraction apparatus to the vibrative method of the sleeve pipe of extraction apparatus.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310014908.3A CN103923831B (en) | 2013-01-15 | 2013-01-15 | For extracting the instruments and methods of biologically active substance |
| HK15100445.4A HK1199902A1 (en) | 2013-01-15 | 2015-01-15 | Instrument and method for extraction of bioactive substances |
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| CN201310014908.3A CN103923831B (en) | 2013-01-15 | 2013-01-15 | For extracting the instruments and methods of biologically active substance |
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| CN105527150B (en) * | 2015-12-10 | 2018-10-23 | 南京医科大学 | A kind of high-throughput magnetic extracting and enriching device and enrichment method |
| CN106318847A (en) * | 2016-08-22 | 2017-01-11 | 杭州奥盛仪器有限公司 | Nucleic acid extraction equipment capable of eccentric oscillation |
| CN106381259B (en) * | 2016-09-27 | 2018-09-11 | 深圳市港科深研生物科技有限公司 | The vitro extraction device of peripheral blood mononuclear cells and the vitro extraction method of peripheral blood mononuclear cells |
| WO2019071450A1 (en) * | 2017-10-11 | 2019-04-18 | Shanghai Rendu Biotechnology Co., Ltd. | Automated nucleic acid sample preparation, detection, and analysis system |
| CN109266547A (en) * | 2018-11-27 | 2019-01-25 | 嘉兴领宇科技有限公司 | Nucleic acid-extracting apparatus |
| CN112940928A (en) * | 2021-02-19 | 2021-06-11 | 清华大学 | Fully-integrated pathogen nucleic acid analyzer |
| CN114366613B (en) * | 2022-01-30 | 2024-04-19 | 宁波市第一医院 | Partition stone removing bed and working method thereof |
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| KR100445560B1 (en) * | 2001-10-31 | 2004-08-21 | (주)바이오넥스 | Method of manufacturing kit for isolating nucleic acids or biological materials, kit manufactured by the method, and apparatus using the kit |
| KR100483684B1 (en) * | 2003-01-29 | 2005-04-18 | (주)바이오넥스 | Kit for separating and purifying nucleic acids or various biological materials, and system for automatically performing separation or purification of biological materials using the same |
| CN2890059Y (en) * | 2006-01-24 | 2007-04-18 | 陈晓汀 | Nucleic acid extracting instrument |
| CN101143884B (en) * | 2007-10-15 | 2011-04-27 | 吉林大学 | Nucleic acid automatic extracting instrument |
| CN201510887U (en) * | 2009-05-14 | 2010-06-23 | 上海科华实验系统有限公司 | Liquid-transferring device with an automatic imbibition head removing mechanism |
| CN203174103U (en) * | 2013-01-15 | 2013-09-04 | 北京金麦格生物技术有限公司 | Instrument for extracting bioactive substance |
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