CN203174085U - Vibrating mechanism for instrument for extracting bioactive substance by magnetic bar method - Google Patents
Vibrating mechanism for instrument for extracting bioactive substance by magnetic bar method Download PDFInfo
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- CN203174085U CN203174085U CN 201320021714 CN201320021714U CN203174085U CN 203174085 U CN203174085 U CN 203174085U CN 201320021714 CN201320021714 CN 201320021714 CN 201320021714 U CN201320021714 U CN 201320021714U CN 203174085 U CN203174085 U CN 203174085U
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- needle
- sleeve pipe
- end block
- lever arm
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Abstract
The utility model provides a vibrating mechanism for an instrument for extracting a bioactive substance by a magnetic bar method. The vibrating mechanism comprises a lever arm, a moveable pivot and a rotating mechanism. According to the arrangement, the lowest point of amplitude is not changed no matter how to change the amplitude of vibration. The utility model further provides a bioactive substance extraction instrument using the vibrating mechanism. The vibrating mechanism and the bioactive substance extraction instrument can adjust vibration frequency and amplitude randomly, can greatly enlarge the adjustment range of the vibration frequency, and have the advantages of low noise and long life.
Description
Technical field
The utility model relates to biology and apparatus field.Concrete, the utility model provides a kind of vibrating mechanism for bar magnet method biologically active substance extraction apparatus.
Background technology
Extract cell in multiple field such as modern molecular biology research, clinical disease diagnosis, blood transfusion, food safety detection, and biologically active substances such as protein, nucleic acid has vital role.Wherein, the biologically active substance compound that nucleic acid is aggregated into by many Nucleotide is one of base substance of life.Nucleic acid extensively is present in all animals, vegetable cell, microorganism, in the organism.According to the chemical constitution difference, nucleic acid can be divided into Yeast Nucleic Acid, is called for short RNA and thymus nucleic acid, is called for short DNA.DNA is the essential substance basis that stores, copies and convey hereditary information, and RNA plays an important role in protein building-up process.Development of biology is maked rapid progress, along with the application of round pcr in every field, comprise the application of medical science aspect disease detection, the detection of agriculture aspect transgenosis and other a lot of aspects etc., need method and the instrument of high-throughout sample biologically active substance extraction and full-automatic biologically active substance extraction.
The paramagnetic particle method utilization that developed recently gets up can the specific recognition cell, the magnetic bead of protein or nucleic acid extracts biologically active substance.Magnetic bead can be combined specifically with nucleic acid molecule, and under the effect in magnetic field, the active macromole from blood, animal tissues, food, pathogenic micro-organism equal samples is separated then.
The paramagnetic particle method automatic extracting instrument is divided into bar magnet method and suction method again.Suction method is to be undertaken by automatic pipetting device.Be example with paramagnetic particle method nucleic acid extraction instrument, can add lysate, magnetic bead absorption by automatic pipetting device; Take solution away, add rinsing liquid, rinsing liquid is taken in magnetic bead absorption away, adds elution buffer.Do not take magnetic bead away because suction method exists when removing waste liquid, pipettor can not lean on magnetic bead too near, in case the problem that magnetic bead is taken out together with waste liquid influences elution efficiency and purity.
It is the technology that developed recently gets up that the bar magnet method is extracted, and its principle is that magnetic separating device is immersed liquid, makes in the mixture and can be concentrated on the separating device surface by the particle of magnetic attraction by the action of a magnetic field.Wherein in a kind of mode, magnetic separating device is a sleeve pipe, makes sleeve pipe produce magnetic by inserting bar magnet, attract to be adsorbed onto sleeve surface by the particle of magnetic attraction in the mixture, when bar magnet was detached, sleeve pipe lost magnetism, and was broken away from sleeve pipe by the particle of magnetic attraction.The flux of bar magnet method nucleic acid extraction instrument has 8,16,32,96 passages usually.The mechanism that drives bar magnet can drive bar magnet and move up and down in sleeve pipe, helps cracking and rinsing and the purifying of leaching process.
Summary of the invention
The utility model provides a kind of vibrating mechanism of the biologically active substance extraction apparatus for the bar magnet method, and the biologically active substance that uses described vibrating mechanism extraction apparatus also is provided.Vibrating mechanism of the present utility model and extraction apparatus can be applicable to multiple fields such as clinical disease diagnosis, transfusion safety, medical jurisprudence evaluation, environmental microorganism detection, food safety detection, molecular biology research, be used for extracting and purifying biological active substance, for example cell, albumen and nucleic acid.
Concrete, the utility model provides a kind of vibrating mechanism for the biologically active substance extraction apparatus.Described extraction apparatus has needle and sleeve pipe, and needle can vertically insert and break away from sleeve pipe, and in addition, vibrating mechanism can produce vibration, drives the sleeve pipe up-down vibration of described extraction apparatus,
It is characterized in that described vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, wherein said rotating mechanism has wheel disc and axle center, when described rotating mechanism rotated around the axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thereby produced vibration.Aspect one of the present utility model, the lever arm of vibrating mechanism of the present utility model, anyway the amplitude size of the vibration that being arranged so that of movable fulcrum and rotating mechanism produces changes, and its lower-most point of amplitude of vibration of exporting to sleeve pipe is constant.
Rotating mechanism in the vibrating mechanism of the present utility model has wheel disc and the axle center of ad hoc type line profile, and when these rotatable parts rotated around the axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, produces and the output vibration.Aspect one of them, described rotating mechanism is eccentric rotary mechanism of the present utility model, and the axle center of its rotatable parts is eccentric rotary mechanism not at the center.Eccentric rotary mechanism can be eccentric wheel etc.
In the utility model, the point that is formed into the shortest distance in its axle center on the profile outer rim of described rotating mechanism (particularly eccentric rotary mechanism) is called nearest profile point.In the utility model, the working order of vibrating mechanism refers to the rotating mechanism of vibrating mechanism around its axle center rotation, the state when the outer rim promotion lever arm of its wheel disc rotates around balance pivot.In the utility model, when the nearest profile point of rotating mechanism described in the vibrating mechanism produces pushing effect (the nearest profile point that is generally rotating mechanism contacts with lever arm) to lever arm, lever arm rotates around balance pivot in the vibrating mechanism, can supply this moment lever arm to be called the lowest amplitude position of the fulcrum around the position of rotating lever fulcrum.At a kind of embodiment of the present utility model, lever arm in the rotating mechanism of the present utility model, being set to of movable fulcrum and rotating mechanism descended in working order, and the variation of the position of movable fulcrum is limited to the lowest amplitude position of the fulcrum.Thus, can so that no matter how the displacement that promotes lever arm of the rotating mechanism of vibrating mechanism changes, also no matter how the position of movable fulcrum on lever arm is adjusted, also namely no matter vibrating mechanism produce and how the amplitude of the vibration of output changes, the lower-most point of the amplitude of the vibration of its output is all constant, thereby how the amplitude that makes vibrating mechanism no matter export to sleeve pipe changes, the lower-most point of its amplitude is constant, and also namely the lower-most point of the amplitude of the pipe box of the extraction apparatus of feasible employing vibrating mechanism of the present utility model is constant.Usually, vibrating mechanism is by fixing some output amplitude on the lever arm, and this point is normally at the opposite side of the fulcrum that relatively rotates mechanism and lever arm point of contact.No matter how the vibrating mechanism amplitude of exporting to casing support changes, constant in the lower-most point of the amplitude of this point of fixity, makes namely that also the lower-most point of amplitude of pipe box of the extraction apparatus that adopts vibrating mechanism of the present utility model is constant.
The rotating mechanism that uses in the vibrating mechanism of the present utility model can be eccentric rotary mechanism, and it has the wheel disc of axle center (being turning point) and ad hoc type line profile, and its axle center is not or not the center.Preferably, described rotating mechanism can adopt circular as profile, is eccentric wheel.It is easily manufactured to adopt circular wheel to have, and technology is simple, and the amplitude of output vibration is easy to advantages such as adjusting.In addition, the cam with non-central axle center also can be used as eccentric rotary of the present utility model mechanism.
Have central hub but can also can be used for vibrating mechanism of the present utility model by vibrative rotating mechanism, for example outline is oval cam.
In vibrating mechanism of the present utility model, can be fulcrum contact or embed the groove that is arranged on the lever arm or hollow groove etc. with the lower edge of lever arm for movable fulcrum and the way of contact of lever arm.
In a kind of embodiment of the utility model vibrating mechanism, movable fulcrum is a plurality of fulcrums that can independently separate or contact with lever arm, comes in contact with lever arm respectively as required and forms single fulcrum, can produce the vibration with particular amplitude thus.For example, can be below lever arm or the side a plurality of fulcrum piece or fulcrum shafts of can lifting or moving horizontally are set, but each fulcrum piece or fulcrum shaft lifting contact or mobile lever arm lower edge or the groove of embedding, the independent fulcrum that forms respectively with the lever arm lower edge.
In another embodiment of vibrating mechanism of the present utility model, movable fulcrum is set to and can or be arranged on groove on the lever arm or mobile fulcrum that hollow groove moves, the vibration that can produce the amplitude with continuous variable thus along the lower edge of lever arm.For example, movable fulcrum is mounted in the fulcrum shaft on the mobile seat, and fulcrum shaft contacts with the lower edge of lever arm, or inserts groove or hollow groove on the lever arm, moves the formation fulcrum at mobile driving lower edge lever arm.
Lever arm preferably is set to linear lever arm in the utility model vibrating mechanism.Described lever arm can have the groove parallel with its upper surface or hollow groove, can be used for for the fulcrum contact.Mobile fulcrum can move along the edge (lower edge) of lever arm or groove or the hollow groove of being located on the lever arm, and its travel direction is the axial direction due of lever arm.In this case, the vibrating mechanism axle center that is set to eccentric rotary mechanism equates with the distance of described axle center to lever arm to the minor increment of profile outer rim.When eccentric rotary mechanism was eccentric wheel, vibrating mechanism was set to eccentric minimum radius (being the minor increment that wheel rim is arrived in the axle center) and equates with the distance of its axle center to lever arm.In this case, the radial direction of eccentric wheel minimum radius is vertical with the travel direction (being the axial of lever arm) that lever moves fulcrum.
Lever arm also can be the lever arm of broken-line type or shaped form in the utility model vibrating mechanism.
In vibrating mechanism of the present utility model aspect another, mobile fulcrum comprises traversing carriage 23, traversing carriage driving mechanism 24 and mobile fulcrum shaft 37, lever arm has the hollow groove parallel with its upper surface, mobile fulcrum shaft passes hollow groove, mobile in hollow groove under the traversing carriage driving mechanism drives, the mobile fulcrum of formation lever.
Aspect of vibrating mechanism of the present utility model, lever arm has vibrating wheels 25, and described vibrating wheels is given sleeve pipe with conduct vibrations, and described vibrating wheels and rotating mechanism lay respectively at the both sides of mobile fulcrum.Aspect one of the present utility model, described vibrating wheels is to contact conduction vibration with the sleeve pipe hoisting appliance.
Aspect of vibrating mechanism of the present utility model, described vibrating mechanism also comprises sleeve pipe travel mechanism (16), sleeve pipe travel mechanism comprises casing support (6) and sleeve pipe hoisting appliance (18), sleeve pipe removably is fixed on the casing support, wherein, by moving up and down of sleeve pipe hoisting appliance, make sleeve pipe move up and down.
Aspect of vibrating mechanism of the present utility model, wherein casing support (6) comprises casing support end block (35) and casing support arm (5), and sleeve pipe removably is fixed on the casing support arm; Described sleeve pipe hoisting appliance comprises the sleeve pipe lifting end block (36) that is arranged on casing support end block below, when sleeve pipe lifting end block moves upward, promoting the casing support end block moves upward, thereby promoting casing support moves upward, when the lifting end block moved downward, the casing support end block moved downward synchronously according to action of gravity and sleeve pipe lifting end block.
Aspect of vibrating mechanism of the present utility model, wherein said sleeve pipe lifting end block and casing support are separable.
Aspect of vibrating mechanism of the present utility model, wherein casing support end block below arranges cover tube vibration push rod (26).
Aspect of vibrating mechanism of the present utility model, described vibrating mechanism also comprises needle travel mechanism (48), needle travel mechanism comprises needle support (8) and needle rising mechanism (33), needle removably is fixed on the needle support, wherein, by moving up and down of needle rising mechanism, make needle can vertically insert and break away from sleeve pipe.
Aspect of vibrating mechanism of the present utility model, wherein needle support (8) comprises needle support end block (9) and needle supported arm (34), and needle removably is fixed on the needle supported arm; Described needle rising mechanism comprises that the needle that is arranged on needle support end block below promotes end block (17), when needle promotes end block and moves upward, promotes needle support end block and moves upward, and moves upward thereby promote the needle support.
Aspect of vibrating mechanism of the present utility model, wherein needle promotion end block (17) is separable with needle support (8), when the needle support contacts with casing support in the process that descends, needle promotes end block to be separated with the needle support, and the needle support is supported by casing support and together moves with casing support.
Aspect of vibrating mechanism of the present utility model, wherein casing support has at least one casing support arm, and preferred, casing support has two casing support arms, but has the bushing slot of fixed sleeving on the supported arm.
Aspect of vibrating mechanism of the present utility model, wherein the needle support has at least one needle supported arm, and preferred, the needle support has two supported arms, has the fixedly needle groove of needle on the supported arm.
Aspect of vibrating mechanism of the present utility model, it is used for the biologically active substance extraction apparatus, and described biologically active substance is cell, protein or nucleic acid.Aspect one of the present utility model, wherein said biologically active substance extraction apparatus is the instrument of extraction and purification of nucleic acid.
Another aspect of the present utility model has provided a kind of for the instrument that extracts biologically active substance, comprising:
Shell,
Shell and base plate 1,
Worktable 15 has the sample preparation district on it,
Sleeve pipe 4 and needle 7, needle can vertically insert and break away from sleeve pipe,
With
Vibrating mechanism 19 of the present utility model as described before, described vibrating mechanism can produce and export vibration, makes sleeve pipe carry out up-down vibration, wherein,
Vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, and wherein said rotating mechanism has wheel disc and axle center, and when described rotating mechanism rotated around the axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thereby produced vibration.The feature of vibrating mechanism of the present utility model also is, described lever arm, and anyway the amplitude size of the vibration that being arranged so that of movable fulcrum and rotating mechanism produces changes, and its lower-most point of vibration of exporting to sleeve pipe is constant.Thus, no matter vibrating mechanism produces and how the amplitude of the vibration of output changes, and also namely no matter how its conduction changes for amplitude of casing support, and the lower-most point of the vibration of the pipe box of instrument of the present utility model is constant.
Rotating mechanism in the vibrating mechanism of the utility model instrument has wheel disc and the axle center of ad hoc type line profile, and when these rotatable parts rotated around the axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, produces and the output vibration.Aspect one of them, described rotating mechanism is eccentric rotary mechanism of the present utility model, and the axle center of its rotatable parts is eccentric rotary mechanism not at the center.Eccentric rotary mechanism can be eccentric wheel etc.
At a kind of embodiment of the present utility model, in the instrument of the present utility model, the lever arm of vibrating mechanism, being set to of movable fulcrum and rotating mechanism descended in working order, and the variation of the position of movable fulcrum is limited to the lowest amplitude position of the fulcrum.Thus, can so that no matter how the displacement that promotes lever arm of the rotating mechanism of vibrating mechanism changes, also no matter how the position of movable fulcrum on lever arm is adjusted, also namely no matter vibrating mechanism produce and how the amplitude of the vibration of output changes, the lower-most point of the amplitude of its output is all constant, thereby how the amplitude that makes vibrating mechanism no matter export to sleeve pipe changes, and the lower-most point of its amplitude is constant, makes namely that also the lower-most point of amplitude of pipe box of instrument of the present utility model is constant.Usually, vibrating mechanism is by fixing some output amplitude on the lever arm, and this point is normally at the opposite side of the fulcrum that relatively rotates mechanism and lever arm point of contact.No matter how the vibrating mechanism amplitude of exporting to casing support changes, constant in the lower-most point of the amplitude of this point of fixity, makes namely that also the lower-most point of amplitude of pipe box of instrument of the present utility model is constant.
The rotating mechanism that uses in the utility model instrument can be eccentric rotary mechanism, and it has the wheel disc of axle center (being turning point) and ad hoc type line profile, and its axle center is not or not the center.Preferably, described rotating mechanism can adopt circular as profile, is eccentric wheel.It is easily manufactured to adopt circular wheel to have, and technology is simple, and the amplitude of output vibration is easy to advantages such as adjusting.In addition, the cam with non-central axle center also can be used as eccentric rotary of the present utility model mechanism.
Have central hub but can also can be used for the utility model by vibrative rotating mechanism, for example outline is oval cam.
In the utility model instrument, can be fulcrum contact or embed the groove that is arranged on the lever arm or hollow groove etc. with the lower edge of lever arm for movable fulcrum and the way of contact of lever arm.
In a kind of embodiment of the utility model instrument, movable fulcrum is a plurality of fulcrums that can independently separate or contact with lever arm, comes in contact with lever arm respectively as required and forms single fulcrum, can produce the vibration with particular amplitude thus.For example, can be below lever arm or the side a plurality of fulcrum piece or fulcrum shafts of can lifting or moving horizontally are set, but each fulcrum piece or fulcrum shaft lifting contact or mobile lever arm lower edge or the groove of embedding, the independent fulcrum that forms respectively with the lever arm lower edge.
In another embodiment of the utility model instrument, movable fulcrum is set to and can or be arranged on groove on the lever arm or mobile fulcrum that hollow groove moves, the vibration that can produce the amplitude with continuous variable thus along the lower edge of lever arm.For example, movable fulcrum is mounted in the fulcrum shaft on the mobile seat, and fulcrum shaft contacts with the lower edge of lever arm, or inserts groove or hollow groove on the lever arm, moves the formation fulcrum at mobile driving lower edge lever arm.
Lever arm preferably is set to linear lever arm.Described lever arm can have the groove parallel with its upper surface or hollow groove, can be used for for the fulcrum contact.Mobile fulcrum can move along the edge (lower edge) of lever arm or groove or the hollow groove of being located on the lever arm, and its travel direction is the axial direction due of lever arm.In this case, the vibrating mechanism axle center that is set to eccentric rotary mechanism equates with the distance of described axle center to lever arm to the minor increment of profile outer rim.When eccentric rotary mechanism was eccentric wheel, vibrating mechanism was set to eccentric minimum radius (being the minor increment that wheel rim is arrived in the axle center) and equates with the distance of its axle center to lever arm.In this case, the radial direction of eccentric wheel minimum radius is vertical with the travel direction (being the axial of lever arm) that lever moves fulcrum.
Lever arm also can be the lever arm of broken-line type or shaped form.
At instrument of the present utility model wherein aspect another, mobile fulcrum comprises traversing carriage 23, traversing carriage driving mechanism 24 and mobile fulcrum shaft 37, lever arm has the hollow groove parallel with its upper surface, mobile fulcrum shaft passes hollow groove, mobile in hollow groove under the traversing carriage driving mechanism drives, the mobile fulcrum of formation lever.
Aspect of the utility model instrument, lever arm has vibrating wheels 25, and described vibrating wheels contacts with the sleeve pipe hoisting appliance, conduction vibration, and described vibrating wheels and rotating mechanism lay respectively at the both sides of mobile fulcrum.
Aspect of the utility model instrument, in the above-mentioned instrument of the present utility model, needle support 8 comprises needle support end block 9 and needle supported arm 34, and needle removably is fixed on the needle supported arm; Described needle rising mechanism comprises that the needle that is arranged on needle support end block below promotes end block 17, when needle promotes end block and moves upward, promotes needle support end block and moves upward, and moves upward thereby promote the needle support.Of the present utility model aspect another, needle promotion end block 17 is separable with needle support 8 in the above-mentioned instrument, when the needle support contacts with casing support in the process that descends, needle promotes end block to be separated with the needle support, and the needle support is supported by casing support and together moves with casing support.
Aspect of the utility model instrument, casing support 6 comprises casing support end block 35 and casing support arm 5, and sleeve pipe removably is fixed on the casing support arm; Described sleeve pipe hoisting appliance comprises the sleeve pipe lifting end block 36 that is arranged on casing support end block below, when sleeve pipe lifting end block moves upward, promoting the casing support end block moves upward, thereby promoting casing support moves upward, when the lifting end block moved downward, the casing support end block moved downward synchronously according to action of gravity and sleeve pipe lifting end block.Aspect another, sleeve pipe lifting end block and casing support are separable described in the above-mentioned instrument of the present utility model.In addition, in a kind of embodiment of the present utility model, the casing support end block of above-mentioned instrument below arranges cover tube vibration push rod 26, and it is connected conduction vibration with vibrating mechanism 19.
Aspect of the utility model instrument, also have for the mechanism rack (13) that supports described needle travel mechanism (48) and sleeve pipe travel mechanism (16).
Aspect of the utility model instrument, casing support has at least one casing support arm, and preferred, casing support has two casing support arms, but has the bushing slot of fixed sleeving on the supported arm.
Aspect one of the present utility model, in the above-mentioned instrument of the present utility model, the needle support has at least one needle supported arm, and preferred, the needle support has two supported arms, has the fixedly needle groove of needle on the supported arm.
Aspect one of the present utility model, in the above-mentioned instrument of the present utility model, worktable can move horizontally.Wherein the sample preparation district can comprise workspace, sample cell or sample well, or its combination.Aspect another, described sample preparation district has bottoming hole of the present utility model, its below configuration well heater.
Aspect one of the present utility model, needle is permanent magnet material described in the above-mentioned instrument of the present utility model.
Aspect one of the present utility model, sleeve pipe is nonmagnetic substance described in the above-mentioned instrument of the present utility model.
Above-mentioned instrument of the present utility model can be used for extracting biologically active substance, for example cell, protein or nucleic acid.
Above-mentioned instrument of the present utility model can be for being used for the instrument of extraction and purification of nucleic acid.
Description of drawings
By reading the detailed description of doing with reference to the following drawings that non-limiting example is done, it is more obvious that other features, objects and advantages of the present utility model will become:
Fig. 1 is the first direction stereographic map according to a preferred embodiment of the utility model;
Fig. 2 is the second direction stereographic map according to a preferred embodiment of the utility model;
Fig. 3 is the side cross-sectional schematic according to a preferred embodiment of the utility model;
Fig. 4 is the side cross-sectional schematic according to a preferred embodiment of the utility model;
Fig. 5 is the sectional block diagram according to a preferred embodiment of the utility model.
Reference numeral
1 base plate
2 heated sample grooves
3 non-heating work districts
4 sleeve pipes
5 casing support arms
6 casing supports
7 needles
8 needle supports
9 needle support end blocks
10 optical axises
11 motors one
12 motors two
13 mechanism racks
14 mechanism's platforms
15 traversers
16 sleeve pipe travel mechanisms
17 needles promote end block
18 sleeve pipe hoisting appliances
19 vibrating mechanisms
20 eccentric wheel drive-motor
21 eccentric wheels
22 lever arms
23 traversing carriages
24 traversing carriage driving mechanisms
25 vibrating wheels
26 cover tube vibration push rods
31 screw mandrels one
32 screw mandrels two
33 needle rising mechanisms
34 needle supported arms
35 casing support end blocks
36 sleeve pipe lifting end blocks
37 move fulcrum shaft
48 needle travel mechanisms
Embodiment
In order to understand better and to explain the utility model, below with reference to accompanying drawings the utility model is described in further detail.
Fig. 1 is the first direction stereographic map according to the inside of the instrument that is used for the extraction biologically active substance of the vibrating mechanism that adopts preferred embodiment of the present utility model.Adopt the instrument of vibrating mechanism of the present utility model to can be used for extracting and purification of nucleic acid.This instrument has shell (not illustrating at accompanying drawing) and base plate 1, needle 7 and needle travel mechanism 48, sleeve pipe 4 and sleeve pipe travel mechanism 16, be used for supporting the support 13 of described needle travel mechanism and sleeve pipe travel mechanism, and for generation of vibration, make sleeve pipe travel mechanism carry out the vibrating mechanism 19 of up-down vibration.
Shell (not illustrating at accompanying drawing) can be spaced apart with instrument internal and atmosphere, in order to avoid sample contamination and damage parts.Housing face-plate is generally metallic substance and makes.Shell can have the sample window that can open and close, and is used for putting into and taking out sample.Shell can have the viewing window made from transparent material.Sample window and viewing window can be same parts.Shell can have the external apparatus interface that is connected with peripheral equipment in addition, the display screen of operation display interface, various manipulation of switches, button or button etc.
Sleeve pipe is the pipe with closed bottom and hollow tube chamber.Sleeve pipe can have for example elongated cylinder form.The material of sleeve pipe is normally nonmagnetic.The selection of its material and thickness and be designed to not weakened field or only influence magnetic field a little, but need guarantee when inserting needle, can attract in the outer generation of sleeve pipe the magnetic field of the biological sample of magnetic bead or band magnetic bead.
Needle can vertically insert and break away from sleeve pipe.The bottom of needle (namely inserting an end of sleeve pipe) has magnetic.When needle inserts and contact or during near sleeve bottom, can produce magnetic field outside sleeve pipe, thereby realize that sleeve pipe is to the magnetic bead in the outer solution of sleeve pipe or absorption and the disengaging of being with the biological sample of magnetic bead.In the present embodiment, the bottom of needle has permanent magnet, has magnetic thus.In other embodiment of the present utility model, needle can produce magnetic (electromagnet) by electromagnetic action.
Fig. 2 is the second direction stereographic map of the instrument that is used for the extraction biologically active substance of above-mentioned employing vibrating mechanism of the present utility model.
As depicted in figs. 1 and 2, described have the worktable 15 that can move horizontally for the instrument that extracts biologically active substance.Has the sample preparation district on the worktable 15 that can move horizontally as shown in Figure 2.The sample preparation district can comprise workspace, sample cell, sample well, or its combination.The sample preparation district is used for arranging sample cell or the sample well that can handle sample, or is used for placing the vessel of load sample, as type culture plates such as single or single centrifuge tube, 24 orifice plates or 96 orifice plates, also can put the non-standard culture plate that needs.
In described instrument, worktable has two groups of sample preparation districts with corresponding two groups of needles and cannula system.Every group of sample preparation district comprises the non-heating work district 3 in two groups of heated sample grooves 2 and these two groups of sample cells respectively.The heated sample groove can insert single or single containers such as centrifuge tube.Single or single containers such as centrifuge tube can be put by non-heating work district, also can put type culture plates such as 24 orifice plates or 96 orifice plates, can also put the non-standard culture plate that needs.Worktable 15 is by the driving mechanisms control that is arranged at its below, can move in the horizontal direction, make the sample preparation district move to the below of sleeve pipe, sleeve pipe can vertically insert and break away from the sample cell in the sample preparation district, or the test tube or the EP that are fixed on sample cell manage, or the hole of culture plate.
Described have needle 7 and needle travel mechanism 48 for the instrument that extracts biologically active substance.Needle travel mechanism 48 comprises needle support 8 and needle rising mechanism 33.
Needle rising mechanism comprises that needle promotes end block 17, motor 12, and leading screw 2 32 and nut transmission pair, motor drives promotion end block 17 by leading screw and nut transmission pair and moves upward.Needle promotes end block 17 and is arranged on needle support end block 9 belows.Needle promotes end block and can contact with needle support end block.When motor drives when promoting end block and moving upward by leading screw and nut transmission pair, needle promotes end block and contact with needle support end block, and promotion needle support end block moves upward, thereby promotion needle support moves upward.
Described also have sleeve pipe 4 and sleeve pipe travel mechanism 16 for the instrument that extracts biologically active substance.Sleeve pipe travel mechanism comprises casing support 6 and sleeve pipe hoisting appliance 18.Casing support comprises casing support end block 35 and casing support arm 5.At the instrument that is used for extracting biologically active substance of diagram preferred embodiment of the present utility model, casing support has two casing support arms that be arranged in parallel.Casing support also can have only a casing support arm, also can be above two.Sleeve pipe removably is fixed on the casing support arm.The quantity of the fixable sleeve pipe of each casing support arm can be adjusted as required.The quantity of sleeve pipe can be adjusted as required, and common every group is 1-12 root sleeve pipe.Common is 4,6 or 12 sleeve pipes.At another embodiment, but casing support arm fixed sleeving group, and each sleeve pipe group has many bar casing tubes, 24 of corresponding culture plate sleeve pipes (spread pattern is 4 * 6) for example, 96 sleeve pipes (spread pattern is 8 * 12) etc.
The sleeve pipe hoisting appliance comprises sleeve pipe lifting end block 36, motor 11, leading screw 1 and nut transmission pair.Motor drives sleeve pipe lifting end block by leading screw and nut transmission pair and moves up or down.Sleeve pipe lifting end block is arranged on casing support end block below.Sleeve pipe lifting end block can with the contacting of casing support end block.When motor drove sleeve pipe lifting end block and moves upward by leading screw and nut transmission pair, sleeve pipe lifting end block can contact with the casing support end block, and promoted the casing support end block and move upward, thereby the promotion casing support moves upward.When motor moved downward by leading screw and nut transmission pair drive sleeve pipe lifting end block, the casing support end block moved downward synchronously according to action of gravity and sleeve pipe lifting end block, moves downward thereby drive casing support.
Fig. 3 and Fig. 4 are described side cross-sectional schematic for the instrument that extracts biologically active substance.From Fig. 3 and Fig. 4 as can be seen, sleeve pipe lifting end block 36 is separable with casing support end block 35, the vibration that realizes sleeve pipe thus with move up and down and can be controlled by different institutions independently.
Sleeve pipe lifting end block is arranged at casing support end block 35 belows.When motor moves upward by leading screw and nut transmission pair drive sleeve pipe lifting end block, promote casing support and move upward.When motor moved downward by leading screw and nut transmission pair drive sleeve pipe lifting end block, the casing support end block moved downward synchronously according to action of gravity and sleeve pipe lifting end block, moves downward thereby drive casing support.
Adopting the instrument that is used for extracting biologically active substance of vibrating mechanism of the present utility model, as Fig. 2 and shown in Figure 5, casing support end block below arranges cover tube vibration push rod 26, and it is connected with vibrating mechanism, conduction vibration.
For the instrument that extracts biologically active substance, sleeve pipe lifting end block is arranged on casing support end block below illustrated, and the length of its upper surface is identical with the lower surface of casing support end block with width, can rise sleeve pipe lifting end block and support and pushing effect.In the middle of sleeve pipe lifting end block, has cavity.Cover tube vibration push rod also is arranged on casing support end block below, and its upper surface length is littler than the lower surface of casing support end block with width.Have cavity in the middle of sleeve pipe lifting end block, can allow cover tube vibration push rod pass, the upper end of cover tube vibration push rod contacts with the casing support end block, and the lower end contacts with the vibrating mechanism that is arranged on sleeve pipe travel mechanism below.
Aspect one of the present utility model, it is separable with needle support end block 9 that needle promotes end block 17.Needle promotes a side contacts of end block and needle support end block.When motor moves upward by leading screw and nut transmission pair drive promotion end block, promote the needle support by needle support end block and move upward.Needle is upwards broken away from from sleeve pipe.Moving downward of needle support is according to action of gravity.The needle of instrument of the present utility model promotes end block and the needle support is separable, and this design can realize that the needle termination contacts closely with the abundant of sleeve bottom, has simplified the requirement to automatically controlled part, prevents that needle from impacting the phenomenon generation of sleeve bottom.
Fig. 3 illustrates the working order of described instrument when needle inserts sleeve pipe.Needle 4 inserts sleeve pipe, and its bottom contacts with the bottom of sleeve pipe.Needle promotion this moment end block 17 separates with needle support end block 9, and needle support 8 is supported by casing support 6 and together moves with casing support.Another kind of embodiment is that needle 4 inserts sleeve pipes, arrives predeterminedly near the position of the bottom of sleeve pipe, and needle promoted end block 17 and separated with needle support end block 9 this moment, and the needle support is supported by casing support and together moves with casing support.Predetermined needle bottom arrive near the position of sleeve bottom can by casing support 6 and needle support 8 arrange limit, for example be set to needle supported arm and casing support arm contact, or the male member that arranges on needle supported arm and the casing support arm contacts, and described male member can be the cushioning material preparation.
Fig. 4 illustrates the working order that sleeve pipe separates with needle.Needle promotes a side contacts of end block 17 and needle support end block 9.When motor moves upward by leading screw and nut transmission pair drive needle promotion end block, promote the needle support by needle support end block and move upward, needle separates with sleeve pipe.
In addition, sleeve pipe lifting end block 35 contacts with casing support end block 36, when motor moves upward by leading screw and nut transmission pair drive sleeve pipe lifting end block, promotes casing support and moves upward.When motor moved downward by leading screw and nut transmission pair drive sleeve pipe lifting end block, casing support moved downward with sleeve pipe lifting end block under action of gravity.
Thereby when the biological sample of the magnetic bead that needs sleeve pipe by vibrating outside the wash-out sleeve pipe absorption or band magnetic bead, needle support end block promotes the needle support and moves upward, and sleeve pipe separates with needle.Sleeve pipe lifting end block and casing support end block disengage.Casing support vibrates under the vibrating mechanism effect.
When needing sleeve pipe to insert or during the hole of the centrifuge tube of sample area or culture plate, sleeve pipe lifting end block 35 contacts with casing support end block 36, when motor moved upward by leading screw and nut transmission pair drive sleeve pipe lifting end block, the promotion casing support moved upward and leaves sample area.When motor moved downward by leading screw and nut transmission pair drive sleeve pipe lifting end block, casing support moved downward with sleeve pipe lifting end block under action of gravity, inserts sample area.
In addition, as shown in the figure, described needle travel mechanism and sleeve pipe travel mechanism are arranged on mechanism's platform 14, are supported by mechanism rack 13.
The both sides of needle support end block 9, sleeve pipe lifting end block 36 and casing support end block 35 have perforation, article two, vertically be arranged on the perforation that the optical axis 10 on mechanism's platform passes described needle support end block 9, sleeve pipe lifting end block 36 and casing support end block 35, needle support end block, sleeve pipe lifting end block and casing support end block worked limiting and fixed action.
The both sides that needle promotes end block also have perforation, are passed by other two optical axises.
Adopt the instrument that is used for the extraction biologically active substance of vibrating mechanism of the present utility model to have vibrating mechanism, described vibrating mechanism can produce vibration, makes sleeve pipe travel mechanism drive sleeve pipe and carries out up-down vibration.The sleeve pipe of the instrument of extraction biologically active substance of the present utility model can carry out up-down vibration up to the frequency of 20Hz.Described vibrating mechanism of the present utility model comprises lever arm, mobile fulcrum and eccentric stiffener, and described mobile fulcrum can move along lever arm.Be used for the instrument of extraction biologically active substance in the course of the work, one of them operation requires needle sheath fast vibration up and down.According to different job requirements, this vibration will have different amplitudes and frequency.That adopts the vibrating mechanism for the instrument that extracts biologically active substance of the present utility model is arranged so that amplitude and the frequency vibrated are adjustable; and anyway amplitude is adjusted; the identical unanimity of the starting point of its amplitude (lower-most point); thereby reach the purpose of mechanisms such as protection sheath; with further feature of the present utility model together; make and adopt instrument of the present utility model to have low noise, the advantage of high life.
As shown in Figure 5, driving mechanism of the present utility model comprises linear lever arm 22, traversing carriage 23, mobile fulcrum shaft 37, drive the driving mechanism 24 that traversing carriage moves horizontally, contact and the vibrating wheels 25 of conduction vibration with the cover tube vibration push rod 26 of sleeve pipe hoisting appliance, and eccentric wheel 21 and eccentric wheel drive-motor 20.Lever arm is linear pattern, has the hollow groove parallel with lower edge with its upper limb.Mobile fulcrum shaft passes hollow groove, can be mobile in hollow groove along the lever arm direction under the traversing carriage driving mechanism drives, form the mobile fulcrum of lever.Contact and the vibrating wheels 25 of conduction vibration and the both sides that eccentric wheel lays respectively at mobile fulcrum with the cover tube vibration push rod 26 of sleeve pipe hoisting appliance.Eccentric wheel contacts with lever arm, when eccentric wheel rotates, cause that the lever arm (power arm) in eccentric wheel one side of fulcrum is subjected to displacement, the lever arm of the opposite side of fulcrum (resistance arm) and vibrating wheels are then carried out rightabout displacement synchronously, and namely the rotation around fulcrum takes place lever arm.Eccentric rotation makes vibrating wheels vibrate, and carries out up-down vibration thereby make sleeve pipe travel mechanism drive sleeve pipe.Also namely, the vibrating mechanism for the instrument that extracts biologically active substance of the present utility model produces vibration, the speed of controlled frequency by eccentric stiffener (being eccentric wheel among the embodiment).The size of the controlled damping width of cloth of movement of the position of mobile fulcrum on lever arm also can be controlled the size of amplitude together with eccentric rotating manner.
In the vibrating mechanism of preferred embodiment of the present utility model shown in Figure 5, eccentric wheel directly contacts with the linear pattern lever arm, eccentric minimum radius (being the minor increment that wheel rim is arrived in the axle center) equates with the distance of its axle center to lever arm, the travel direction (being lever arm) that the radial direction of eccentric wheel minimum radius and lever move fulcrum is vertical, at this moment, no matter how fulcrum moves in the hollow groove of lever arm, when the contacting of eccentric wheel and lever arm generation minimum radius, vibrating wheels all is in the lower-most point of its amplitude.No matter how eccentric rotating speed changes, no matter also how the position of mobile fulcrum on lever arm changes, namely regardless of amplitude and the frequency of vibrating wheels, the lower-most point of vibrating wheels all is constant, and the lower-most point of the vibration of sleeve pipe generation just is constant.
In other embodiment of the present utility model, also available cam replaces eccentric wheel to realize the purpose of this utility model.
Extract the instrument of biologically active substance by adopting above-mentioned vibrating mechanism of the present utility model, can adjust vibrational frequency and amplitude arbitrarily, no matter how amplitude is adjusted simultaneously, the minimum start position of various amplitude all is identical, can increase substantially the setting range of vibrational frequency, make whole instrument have low noise, the advantage of high life.
Though describe in detail about example embodiment of the present utility model and advantage thereof; be to be understood that under the situation of the protection domain that does not break away from the restriction of spirit of the present utility model and claims, can carry out various variations, substitutions and modifications to these embodiment.For other examples, when those of ordinary skill in the art should understand easily in keeping the utility model protection domain, the order of processing step can change.
In addition, range of application of the present utility model is not limited to technology, mechanism, manufacturing, material composition, means, method and the step of the specific embodiment of describing in the specification sheets.From disclosure of the present utility model, to easily understand as those of ordinary skill in the art, for the technology, mechanism, manufacturing, material composition, means, method or the step that have existed or be about to later on develop at present, wherein they are carried out the corresponding embodiment cardinal principle identical functions of describing with the utility model or obtain identical substantially result, can use them according to the utility model.Therefore, the utility model claims are intended to these technology, mechanism, manufacturing, material composition, means, method or step are included in its protection domain.
Claims (29)
1. vibrating mechanism that is used for bar magnet method biologically active substance extraction apparatus, the biologically active substance extraction apparatus has needle and sleeve pipe, and needle can vertically insert and break away from sleeve pipe, and described vibrating mechanism can produce vibration, drives described sleeve pipe up-down vibration,
It is characterized in that described vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, wherein said rotating mechanism has wheel disc and axle center, when described rotating mechanism rotated around the axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thereby produced vibration.
2. the described vibrating mechanism of claim 1, wherein said lever arm, being set to of movable fulcrum and rotating mechanism, in working order down, the variation of the position of movable fulcrum is limited to the lowest amplitude position of the fulcrum, the lowest amplitude position of the fulcrum refers to, when the nearest profile point of the described rotating mechanism of vibrating mechanism produces pushing effect to lever arm, and the position of the balance pivot that lever arm rotates about it in the vibrating mechanism.
3. claim 1 or 2 described vibrating mechanisms, wherein said rotating mechanism is eccentric rotary mechanism, its axle center is not or not the center.
4. the described vibrating mechanism of claim 3, wherein said eccentric rotary mechanism is eccentric wheel or the cam with non-central axle center.
5. the described vibrating mechanism of claim 4, wherein said eccentric rotary mechanism is eccentric wheel.
6. claim 1 or 2 described vibrating mechanisms, wherein said movable fulcrum is for moving along the lower edge of lever arm or being arranged on the mobile fulcrum that groove on the lever arm or hollow groove move.
7. claim 1 or 2 vibrating mechanism, wherein said movable fulcrum is a plurality of fulcrums that can independently separate or contact with lever arm, comes in contact with lever arm respectively and forms single fulcrum.
8. claim 1 or 2 described vibrating mechanisms, wherein said lever arm is the linear pattern lever arm.
9. the described vibrating mechanism of claim 8, wherein said lever arm has the groove parallel with upper surface or hollow groove, can be used for contacting with fulcrum.
10. claim 1 or 2 described vibrating mechanisms, wherein said vibrating mechanism comprises linear pattern lever arm (22), mobile fulcrum and eccentric wheel (21), mobile fulcrum can move along lever arm, its travel direction is the axial direction due of lever arm, and wherein eccentric minimum radius equates with the distance of its axle center to lever arm.
11. the described vibrating mechanism of claim 10, wherein mobile fulcrum comprises traversing carriage (23), traversing carriage driving mechanism (24) and mobile fulcrum shaft (37), lever arm has the hollow groove parallel with its upper surface, mobile fulcrum shaft passes hollow groove, mobile in hollow groove under the traversing carriage driving mechanism drives, the mobile fulcrum of formation lever.
12. claim 1 or 2 described vibrating mechanisms, wherein lever arm has vibrating wheels (25), and described vibrating wheels is given sleeve pipe with conduct vibrations, and described vibrating wheels and rotating mechanism lay respectively at the both sides of mobile fulcrum.
13. claim 1 or 2 described vibrating mechanisms, wherein also comprise sleeve pipe travel mechanism (16), sleeve pipe travel mechanism comprises casing support (6) and sleeve pipe hoisting appliance (18), sleeve pipe removably is fixed on the casing support, wherein, by moving up and down of sleeve pipe hoisting appliance, make sleeve pipe move up and down.
14. the described vibrating mechanism of claim 13, wherein casing support (6) comprises casing support end block (35) and casing support arm (5), and sleeve pipe removably is fixed on the casing support arm; Described sleeve pipe hoisting appliance comprises the sleeve pipe lifting end block (36) that is arranged on casing support end block below, when sleeve pipe lifting end block moves upward, promoting the casing support end block moves upward, thereby promoting casing support moves upward, when the lifting end block moved downward, the casing support end block moved downward synchronously according to action of gravity and sleeve pipe lifting end block.
15. the described vibrating mechanism of claim 14, wherein said sleeve pipe lifting end block and casing support are separable.
16. the described vibrating mechanism of claim 15, wherein casing support end block below arranges cover tube vibration push rod (26).
17. claim 1 or 2 described vibrating mechanisms, wherein also comprise needle travel mechanism (48), needle travel mechanism comprises needle support (8) and needle rising mechanism (33), needle removably is fixed on the needle support, wherein, by moving up and down of needle rising mechanism, make needle can vertically insert and break away from sleeve pipe.
18. the described vibrating mechanism of claim 17, wherein needle support (8) comprises needle support end block (9) and needle supported arm (34), and needle removably is fixed on the needle supported arm; Described needle rising mechanism comprises that the needle that is arranged on needle support end block below promotes end block (17), when needle promotes end block and moves upward, promotes needle support end block and moves upward, and moves upward thereby promote the needle support.
19. the described vibrating mechanism of claim 18, wherein needle promotion end block (17) is separable with needle support (8), when the needle support contacts with casing support in the process that descends, needle promotes end block to be separated with the needle support, and the needle support is supported by casing support and together moves with casing support.
20. claim 1 or 2 described vibrating mechanisms, wherein casing support has at least one casing support arm, but has the bushing slot of fixed sleeving on the supported arm.
21. the described vibrating mechanism of claim 20, wherein casing support has two casing support arms.
22. claim 1 or 2 described vibrating mechanisms, wherein the needle support has at least one needle supported arm, has the fixedly needle groove of needle on the supported arm.
23. the described vibrating mechanism of claim 22, wherein the needle support has two supported arms.
24. the described vibrating mechanism of claim 1, wherein said biologically active substance are cell, protein or nucleic acid.
25. the described vibrating mechanism of claim 1, wherein said biologically active substance extraction apparatus is the instrument of extraction and purification of nucleic acid.
26. the described vibrating mechanism of claim 1, wherein said needle are permanent magnet material.
27. the described vibrating mechanism of claim 1, wherein said sleeve pipe are nonmagnetic substance.
28. the described vibrating mechanism of claim 1, wherein said biologically active substance are cell, protein or nucleic acid.
29. the described vibrating mechanism of claim 1, wherein said biologically active substance extraction apparatus is for being used for the instrument of extraction and purification of nucleic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320021714 CN203174085U (en) | 2013-01-15 | 2013-01-15 | Vibrating mechanism for instrument for extracting bioactive substance by magnetic bar method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320021714 CN203174085U (en) | 2013-01-15 | 2013-01-15 | Vibrating mechanism for instrument for extracting bioactive substance by magnetic bar method |
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| CN203174085U true CN203174085U (en) | 2013-09-04 |
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| CN 201320021714 Withdrawn - After Issue CN203174085U (en) | 2013-01-15 | 2013-01-15 | Vibrating mechanism for instrument for extracting bioactive substance by magnetic bar method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103805497A (en) * | 2013-07-16 | 2014-05-21 | 北京金麦格生物技术有限公司 | Device and method of extracting biological active substances by paramagnetic particle method |
| CN103923162A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Vibration mechanism for magnetic rod technique bioactive substance extractor and application thereof |
| WO2019011088A1 (en) * | 2017-07-13 | 2019-01-17 | 上海奇法生物仪器有限公司 | Nucleic acid purifier |
-
2013
- 2013-01-15 CN CN 201320021714 patent/CN203174085U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923162A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Vibration mechanism for magnetic rod technique bioactive substance extractor and application thereof |
| CN103805497A (en) * | 2013-07-16 | 2014-05-21 | 北京金麦格生物技术有限公司 | Device and method of extracting biological active substances by paramagnetic particle method |
| CN103805497B (en) * | 2013-07-16 | 2015-12-02 | 常州金麦格生物技术有限公司 | The apparatus and method of biologically active substance are extracted for paramagnetic particle method |
| WO2019011088A1 (en) * | 2017-07-13 | 2019-01-17 | 上海奇法生物仪器有限公司 | Nucleic acid purifier |
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Owner name: CHANGZHOU GENMAG BIOLOGICAL TECHNOLOGY CO., LTD. Free format text: FORMER NAME: BEIJING GENMAGBIO BIOTECHNOLOGY CO., LTD. |
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| CP03 | Change of name, title or address |
Address after: Wujin District of Jiangsu city of Changzhou province Changzhou 213000 West Road No. 9, Taihu science and technology industry director B1 Patentee after: CHANGZHOU GENMAG BIOTECHNOLOGY CO., LTD. Address before: 100176 No. 3 north Yongchang Road, Daxing District economic and Technological Development Zone, Beijing, 707 Patentee before: Beijing Genmagbio Biotechnology Co., Ltd. |
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