WO2019011088A1

WO2019011088A1 - Nucleic acid purifier

Info

Publication number: WO2019011088A1
Application number: PCT/CN2018/090554
Authority: WO
Inventors: 高爽
Original assignee: 上海奇法生物仪器有限公司
Priority date: 2017-07-13
Filing date: 2018-06-11
Publication date: 2019-01-17
Also published as: CN206956049U

Abstract

A nucleic acid purifier, comprising a mechanical part (1), a control system (2), an operation unit (3), and a casing (4). The mechanical part comprises: a stirring sleeve driving mechanism (5), a bar magnet lifting mechanism (6), a bracket lifting device (8), a bracket translation device (7), a stirring sleeve rack (10), a bar magnet rack (11), a base (12), and a worktable (14). The stirring sleeve driving mechanism (5) is a slider-crank mechanism; a stirring motor rotates in the same direction continuously, and forward and reverse rotation is not required; the stirring sleeve rack (10) fixed on a stirring sleeve slider is driven by the stirring sleeve slider (54) to move up and down along a stirring sleeve guide rail (55) and to stir samples; the bracket lifting device can drive a bracket device (9), and further drive bar magnets (20) and stirring sleeves (21) to go up and down synchronously. The nucleic acid purifier can stir samples at a high frequency and large amplitude, and can effectively improve nucleic acid purification effect. The nucleic acid purifier is simple in structure, reliable in working, low in power consumption, small in vibration and noise and low in cost, and is conveniently maintained.

Description

Nucleic acid purification instrument

Technical field

The invention relates to the field of biological instruments, in particular to a nucleic acid purification automatic device based on nano magnetic beads.

Background technique

Nucleic acids (DNA or RNA) are widely found in animals and plants. In clinical diagnosis and treatment, or forensic identification, or genetic screening and recombination, people need to extract nucleic acids from samples (blood, saliva or other tissues), and the process of extracting nucleic acids is also called nucleic acid purification. The effect of nucleic acid purification directly affects the progress and results of research and diagnosis, so nucleic acid purification technology is an important technology in biotechnology.

A commonly used nucleic acid purification method is a magnetic rod method. The magnetic rod method uses a magnetic rod to adsorb nano magnetic beads, and the nano magnetic beads adsorb nucleic acids to separate nucleic acids from the sample. The working principle of the magnetic bar method is as shown in FIG. 1. The magnetic bar 20, the stirring sleeve 21 and the deep hole plate 22 are arranged in order from top to bottom; wherein the deep hole plate 22 has a set of deep holes, and the deep holes include cracking. The liquid hole 221, the magnetic bead hole 222, the washing liquid hole 223 (a plurality of washing liquid holes may be provided), and the eluent liquid hole 224 respectively store the corresponding reagents. The specific steps of the nucleic acid purification process are: dropping the sample into the lysate to cleave the nucleic acid--collecting the magnetic beads to the lysate well--the magnetic beads are combined with the nucleic acid--collecting the magnetic beads to the washing holes (multiple washings)- The magnetic beads are collected to the elution well--the magnetic beads are recovered after elution, and finally the nucleic acid remains in the eluate. In order to facilitate the automatic operation of the above process, the nucleic acid purifier is required to realize the basic actions as shown in FIG. 1, namely: (1) the up and down movement of the magnetic bar 20 a, (2) the up and down movement of the stirring sleeve 21 and the stirring action c, (3) The vertical movement e after the magnet bar 20 is inserted into the mixing sleeve 21, and (4) the translational action d after the magnetic rod 20 is inserted into the mixing sleeve 21.

Chinese invention patent CN103897987B discloses an automatic nucleic acid extraction device based on nano magnetic beads. The nucleic acid extraction device is mainly composed of a base and three mechanisms mounted on the base. The three mechanisms are: a magnetic bar lifting mechanism, a stirring sleeve driving mechanism and a deep hole plate translation mechanism. The three mechanisms are composed of a stepping motor, a screw nut mechanism, and a sliding bar that supports and guides. The actions achieved by the above three mechanisms are shown in Fig. 1. a: The magnetic bar lifting mechanism drives the magnetic bar 20 to move up and down, the function is to insert or remove the magnetic bar 20 into the stirring sleeve 21; b: the stirring sleeve driving mechanism drives the stirring sleeve 21 slowly. The speed is up and down; c: the stirring sleeve driving mechanism drives the stirring sleeve 21 to quickly stir up and down; d: the deep hole plate translation mechanism drives the deep hole plate 22 to translate left and right (corresponding to the left and right translational movements after the magnetic bar 20 and the stirring sleeve 21 are combined) ; e: The magnetic rod lifting mechanism and the stirring sleeve driving mechanism cooperate to synchronize the movement, and the vertical movement of the magnetic rod 20 and the stirring sleeve 21 after the combination is realized.

The disadvantages of the above nucleic acid extraction device are:

First, the driving mechanism of the stirring sleeve is driven by the stepping motor to drive the stirring sleeve 21 through the screw rod and the screw nut to reciprocate on the sliding rod, thereby realizing the slow moving up and down movement of the stirring sleeve 21 and the quick stirring action. Among them, the stirring action of the stirring sleeve 21 is a high frequency reciprocating movement, and the stepping motor must achieve high frequency forward and reverse rotation.

When the mixing sleeve is stirred, the energy of the motor is converted from the power supply of the system, mainly including the kinetic energy of the rotor of the motor and the electromagnetic energy stored in the armature of the motor. According to Newton's law, the energy of the forward rotation of the motor must disappear, and the motor can be reversed. Conversely, the energy of the motor reversal must disappear and the motor can rotate forward. When the high frequency is reversed, the motor must be braked or decelerated frequently to achieve commutation. Most of the energy of the motor is not used for agitation, it does not form useful work, but is wasted by frequent braking, and thus is inefficient.

According to the law of conservation of energy, energy can neither be produced out of thin air nor disappeared out of thin air. It can only be transformed from one form to another, or from one object to another. Here, the motor has a negative effect due to the energy converted from the positive and negative energy being converted into thermal energy and mechanical energy. Thermal energy causes the motor and the entire system to include elevated circuit temperatures. Mechanical energy causes system vibration and noise.

In order to reduce vibration and noise, existing instruments have to use a heavier instrument base to reduce the resonance frequency, so the instrument is heavier, resulting in increased difficulty and cost of transportation.

Constrained by the dynamic characteristics of the stepper motor, the stepping motor will lose its step when the high frequency is greatly reversed under load, and the energy loss caused by the positive and negative motor is more serious. Therefore, when the stirring frequency is high, it is impossible to stir at the same time. If you want to achieve high frequency and large agitation, you need to use more powerful motors or more expensive motors and components, such as high-power stepper motors, servo motors, ball screws and so on. These components are tens of times more expensive than low-power stepper motors and ordinary screw. Therefore, the prior art stirring sleeve driving mechanism is difficult to achieve a high frequency and a large stirring action. As a result, the sample does not work properly when it is a highly viscous liquid, or if the sample size is large, or the sample impurity content is high. Because high viscosity, or large amounts, or high impurity content samples require high frequency and large amplitude agitation, the nucleic acid can be cleaned. Otherwise, the agitation amplitude does not meet the requirements, which may result in poor purification of the nucleic acid or even failure of extraction.

In the above-mentioned stirring sleeve driving mechanism, the transmission mechanism connected to the stepping motor uses a screw rod and a screw nut, and some instruments use a timing belt transmission mechanism. These two mechanisms are very noisy when moving at high frequencies. Moreover, the screw rod and the screw nut are high-precision transmission components, and are prone to wear failure under long-term frequent reciprocating motion, so the maintenance cost is high. The timing belt will fatigue and break due to long-term frequent reciprocating motion.

Therefore, the above-mentioned stirring sleeve driving mechanism cannot be used for samples requiring high frequency and large agitation, and has high energy consumption, low efficiency, large vibration, high noise, high cost, heavy instrument, and high maintenance rate.

Second, during the nucleic acid purification process, one action is performed multiple times, and the magnetic bar and the stirring sleeve are combined and lifted together, that is, the e action of FIG. In the prior art, the above action is coordinated by the magnetic rod lifting mechanism and the stirring sleeve driving mechanism, and the vertical movement is completed. If the two mechanisms are slightly out of sync, the magnetic beads with nucleic acids will be left outside the deep well, causing the nucleic acid extraction to be interrupted or failed. Therefore, this type of action is risky, resulting in unreliable instrument operation.

Third, in the prior art, the holes in the clamping device of the stirring sleeve frame, the magnetic rod frame and the deep hole plate are matched with the elongated round bar-shaped sliding bar, and the stirring sleeve, the magnetic bar and the deep hole plate are on the sliding bar. Reciprocating. The friction between the outer circle of the slider and the hole is large. And the long slide bar that is erected is only fixed at the end and is easy to bend. Even if the slider has a slight curvature that cannot be recognized by the naked eye, the sliding of the clamping device may not be smooth or even impossible. Therefore, the use of sliders is not a good support and guidance method.

Summary of the invention

In view of the above-mentioned shortcomings or deficiencies of the prior art, the present invention provides a nucleic acid purifying apparatus, wherein the stirring sleeve driving mechanism is a crank slider mechanism, and the motor can rotate in the same direction continuously, without the need of positive and negative reversal, even if the market is the most common and economical. The small power stepping motor can realize the stirring motion, avoiding a series of problems caused by the forward and reverse of the stepping motor in the prior art.

The technical solution of the present invention is

A nucleic acid purifying apparatus comprises a mechanical part, a control system, an operating unit and a casing, wherein the mechanical part is located at a lower part of the nucleic acid purifying device, the control system and the operating unit are located at an upper part of the instrument, the outer casing is wrapped at the outermost part of the instrument, and the mechanical part comprises a stirring sleeve driving mechanism, Stirring frame, magnetic bar lifting mechanism, magnetic bar frame, bracket or support plate, base, work table, table translation device or bracket translation device, the stirring frame is mounted on the stirring sleeve driving mechanism, and the magnetic rod frame is mounted on the magnetic In the rod lifting mechanism, the stirring sleeve driving mechanism and the magnetic rod lifting mechanism are mounted on the bracket or the supporting plate, the bracket or the supporting plate and the working table are mounted on the base, and the working table is disposed under the magnetic rod frame and the stirring sleeve frame; The stirring sleeve driving mechanism comprises a stirring motor, a crank, a connecting rod, a stirring sleeve slider and a stirring sleeve rail. The stirring motor shaft is fixedly connected with the crank, the crank and the connecting rod are rotatably connected at one end, and the other end of the connecting rod and the stirring sleeve slider The rotatably connected, the stirring sleeve is fixed on the stirring sleeve slider, and the stirring sleeve slider is slidably connected with the stirring sleeve rail.

The nucleic acid purifier further includes a bracket lifting device, and the bracket lifting device comprises a bracket lifting motor, an intermediate bracket, a bracket lifting screw, a bracket lifting screw nut, a bracket lifting slider, a bracket lifting rail, and a bracket lifting The motor is mounted on the intermediate bracket, the bracket lifting screw is connected with the bracket lifting motor shaft, the bracket lifting screw nut is set on the bracket lifting screw, the bracket lifting screw nut is connected with the support plate, and the bracket is lifted and slid The block is mounted on the support plate, the bracket lifting rail is fixed on the intermediate bracket, and the bracket lifting slider is slidably connected with the bracket lifting rail.

The bracket translation device of the present invention comprises a bracket translation motor, a bracket translation screw, a bracket translation screw nut, a bracket translation slider, a bracket translation rail, a bracket translation motor mounted on the base, and a bracket translation The lead screw is connected to the bracket translation motor shaft, the bracket translation screw nut is set on the bracket translation screw, the bracket translation screw nut is connected with the bracket lifting device, and the bracket translation slider is mounted on the bracket lifting device. The bracket translation rail is mounted on the base, and the bracket translation slider is slidably coupled to the bracket translation rail.

The magnetic rod lifting mechanism comprises a magnetic rod lifting motor, a magnetic rod lifting screw, a magnetic rod lifting screw nut, a magnetic rod lifting slider, a magnetic rod lifting rail, a magnetic rod lifting motor shaft and a magnetic rod lifting screw, and the magnetic rod lifting The screw nut is set on the magnetic rod lifting screw, the magnetic rod lifting screw nut is connected with the magnetic rod frame, the magnetic rod lifting slider is fixed on the magnetic rod frame, and the magnetic rod lifting slider is slidably connected with the magnetic rod lifting rail .

The stirring sleeve driving mechanism further comprises a blocking piece, a position sensor and a bearing, the blocking piece is fixed on the crank, the position sensor is fixed beside the crank, the blocking piece is a thin plate, the thickness direction thereof passes through the groove on the position sensor, and the bearing is installed in the hole of the connecting rod Inside.

The flap is a right-angled fan.

The slider driving mechanism, the magnetic bar lifting mechanism, the bracket translation device, and the slider and the rail mechanism in the bracket lifting device are both ball linear guide mechanisms.

The crank and connecting rod of the stirring sleeve drive mechanism are made of aluminum alloy.

The ratio of the length of the crank to the connecting rod is less than 1:1, and the frequency of the stirring motor is 0-20 Hz.

Preferably, the ratio of the length of the crank to the connecting rod is 1:4 to 1:6, and the frequency of the stirring motor is 2 to 8 Hz.

The advantages of the present invention over the prior art are:

In the nucleic acid purifying apparatus of the present invention, the stirring sleeve driving mechanism is changed to a crank slider mechanism. The stirring motor only needs to rotate continuously in the same direction, and the lifting and stirring action of the stirring sleeve can be realized without positive and negative reversal. High energy utilization, low system temperature rise, low vibration and noise, and completely eliminate the noise generated by the screw and timing belt during mixing.

The instrument of the invention has small vibration and does not need to increase the weight of the base, so the instrument of the invention has less than 1/2 weight loss compared with the conventional instrument, and is convenient to carry.

The crank slider mechanism of the invention can drive the stirring sleeve to realize a high frequency and a large stirring action, and when the sample is a high viscosity or high impurity liquid, the motor frequency is increased, the stirring amplitude is increased (the crank length is increased), and the stirring can be enhanced. Strength to ensure the quality of nucleic acid purification for specific samples.

The instrument of the invention has high energy utilization rate, and only needs a common low-power motor, a crank-slider transmission mechanism, and a low-power power source, and the component cost is low; the crank slider mechanism is simpler than the screw rod or the timing belt mechanism, More durable.

In the crank slider mechanism of the present invention, the two inner holes of the connecting rod are provided with bearings. And the movement of the stirring sleeve is supported and guided by the ball linear guide mechanism, so the mutual rotation or mutual sliding between the parts in the mechanism is smooth, the parts wear is small, and the instrument has a long service life.

Especially in the case of high frequency and large agitation, the above advantages are more prominent.

The stirring action runs through the entire nucleic acid purification process and is the most influential part of the purification effect. The improvement of the stirring sleeve driving mechanism of the invention plays a key role in improving the performance of the nucleic acid purifying apparatus.

(2) The present invention is specifically provided with a bracket lifting device. The bracket lifting device drives the magnetic rod frame and the stirring sleeve frame mounted on the bracket device to simultaneously lift and lower, thereby ensuring that the magnetic rod and the stirring sleeve are completely synchronized and raised. It avoids the risk of the magnetic beads falling when the two mechanisms are combined and driven, which significantly enhances the reliability of the instrument.

(3) The carriage translating device of the present invention replaces the table translating device disposed under the table in the prior art. The cradle translating device drives the stir bar on the cradle device to translate with the magnetic bar for reliable operation. And the power mechanism of the whole instrument is located at the back of the base, the instrument is safer to use, the layout is more compact and beautiful.

In the stirring sleeve driving mechanism, the magnetic rod lifting mechanism, the bracket lifting device, and the bracket translation device of the present invention, the ball linear guide mechanism is used as the supporting and guiding mechanism of the moving member. The rolling friction between the guide rail and the slider is smaller, which makes the movement of the magnetic rod and the stirring sleeve smoother, more stable and more reliable.

The nucleic acid purifying instrument provided by the invention improves the stirring sleeve driving mechanism, and can adopt high-cost components to perform high-frequency stirring. High energy utilization, low vibration, low noise, light weight, simple structure, convenient maintenance, low instrument cost, long service life and more reliable work.

DRAWINGS

Figure 1 is a schematic diagram of the basic operation of the nucleic acid purifying apparatus;

Figure 2 is an outline view of the nucleic acid purifying apparatus of the present invention;

Figure 3 is a structural view showing the mechanical part of the nucleic acid purifying apparatus of the present invention;

Figure 4 is a plan view showing the mechanical part of the nucleic acid purifying apparatus of the present invention;

Figure 5 is a right side view of the mechanical portion of the nucleic acid purifying apparatus of the present invention;

Figure 6 is a perspective view of the driving mechanism of the stirring sleeve of the nucleic acid purifying apparatus of the present invention;

Figure 7 is a left side view of the stirring sleeve driving mechanism of the nucleic acid purifying apparatus of the present invention;

Figure 8 is a rear elevational view of the stirring sleeve driving mechanism of the nucleic acid purifying apparatus of the present invention;

Figure 9 is a perspective view of the carrier device of the nucleic acid purifying apparatus of the present invention.

Detailed ways

The concept, the specific structure and the technical effects of the present invention will be further described in conjunction with the accompanying drawings in order to fully understand the objects, features and effects of the invention.

As shown in FIG. 2, the nucleic acid purifier is composed of a mechanical part 1, a control system 2, an operation unit 3, and a casing 4. The mechanical part 1 is located at the lower part of the nucleic acid purifier, the control system 2 and the operating unit 3 are located at the upper portion, and the outer casing 4 is wrapped around the outermost part of the instrument. Mechanical part 1 is the core component of the nucleic acid purifier.

In the nucleic acid purifying apparatus of the present invention, the lifting and stirring operation of the stirring sleeve 21 is performed by the stirring sleeve driving mechanism 5. The lifting and lowering of the magnetic bar 20 is performed by the magnetic bar lifting mechanism 6. The stirring sleeve drive mechanism 5 and the magnetic bar lifting mechanism 6 are mounted on the support plate 12 to constitute the bracket device 9. The lifting and lowering of the magnetic bar 20 after the combination of the stirring sleeve 21 and the stirring sleeve 21 are respectively performed by the carriage lifting device 8 and the carriage translation device 7 to drive the carriage device 9. The above mechanism and the table 14 on which the deep hole plate 22 is placed are disposed on the base 13.

As shown in FIGS. 3 and 9, the magnetic bar lifting mechanism 6 is driven by a magnetic bar lifting motor 61 mounted on the upper front surface of the support plate 12, and the magnetic bar lifting motor 61 is fixed to the support plate 12 by a magnetic bar lifting motor mounting plate 65. The magnetic rod lifting motor 61 shaft drives the magnetic rod lifting screw 62 connected underneath to rotate (or the magnetic rod lifting screw 62 is integrated with the magnetic rod lifting motor 61 shaft), and the magnetic rod lifting screw nut 63 is in the magnetic rod lifting wire The rod 62 is driven up and down. The magnetic rod frame 11 is fixedly connected to the magnetic rod lifting screw nut 63, and the magnetic rod lifting rod nut 63 drives the magnetic rod holder 11 to move up and down. The magnetic bar frame 11 is fixedly connected to the magnetic bar lifting and lowering slider 64, and the magnetic bar lifting and lowering slider 64 is slidably connected to the stirring sleeve rail 55 mounted on the supporting plate 12, so that the magnetic bar frame 11 moves up and down along the stirring sleeve guide 55, that is, the figure The operation of a of the magnetic rod holder 11 shown in Fig. 9 is a function of inserting and removing the magnetic rod 20 into the stirring sleeve 21. The sheet 66 fixed on the magnetic rod frame 11 transmits the position signal of the magnetic rod lifting mechanism 6 to the control system 2 when passing through the position sensor 57 mounted on the side of the magnetic rod lifting screw 62 during the movement, and the control system 2 The magnetic bar lifting motor 61 is then controlled to control the lifting and lowering of the magnetic bar 20.

The screw nut mechanism composed of the magnetic rod lifting screw 62 and the magnetic rod lifting screw nut 63 may be a sliding screw nut mechanism, a ball screw nut mechanism, a synchronous belt reciprocating mechanism, a rack and pinion mechanism, and the like. .

As shown in FIG. 6, FIG. 7, and FIG. 8, the power source of the stirring sleeve drive mechanism 5 is the stirring motor 51. The agitating motor 51 is mounted on the lower portion of the back surface of the support plate 12, the shaft of the agitating motor 51 passes through the hole of the support plate 12, the crank 52 is mounted on the shaft of the agitating motor 51, the crank 52 is located on the front surface of the mounting plate, and the other end of the crank 52 is connected to the connecting rod One end of the 53 is hinged by a pin, and the other end of the link 53 is hinged to the stirring frame 10 through a pin. A rolling bearing 58 is mounted in the bore of the connecting rod 53 that cooperates with the pin. The rolling bearing 58 serves to support the pin and reduce wear of the pin. The stirring sleeve 10 is fixedly connected with the stirring sleeve slider 54 , and the stirring sleeve slider 54 is slidably connected with the stirring sleeve rail 55 , so the stirring motor 51 drives the stirring sleeve frame 10 to perform lifting and stirring along the stirring sleeve rail 55 through the crank slider mechanism. That is, the b and c of the stirring sleeve 21 shown in Fig. 6 operate. The action of b and c acts to agitate the sleeve 21 into the deep hole of the deep well plate 22, stir the magnetic beads and liquid, and exit the deep hole. In the cracking and washing stage of nucleic acid purification, the shaft of the stirring motor 51 is continuously rotated 360 degrees, and the amplitude of the stirring frame 10 is equal to twice the length between the two shaft holes of the crank 52. In the elution phase of nucleic acid purification, since the volume of the eluate is small, the amplitude of the agitation rack 10 in the elution phase is smaller than that in the pyrolysis and washing stages, so the agitating motor 51 needs to be positively inverted within a certain angle range in the elution phase. The control signal from the control system 2 to the forward and reverse rotation of the agitating motor 51 comes from the flap 56 mounted on the crank 52 and the photoelectric position sensor 57 below the crank 52 that cooperates with the flap 56. In order to facilitate the positional information of the rotation motor 51 by the photoelectric position sensor 57, the flap 56 is preferably a right-angled sector. The elution phase takes about 1/10 of the total nucleic acid purification time, so the motor reversal at this stage has little effect on the noise of the nucleic acid purifier.

The crank 52 and the connecting rod 53 of the stirring sleeve drive mechanism 5 are made of aluminum alloy, which can effectively reduce the weight of the moving parts and reduce the inertial force of the stirring sleeve 21. The ratio of the length of the crank 52 to the connecting rod 53 is less than 1:1. Because the connecting rod 53 is too long, the mass of the connecting rod 53 is increased, and the space occupied by the mechanism is large. When the connecting rod 53 is too short, the side pressure of the stirring sleeve slider 54 is caused. Increasing, it is preferable that the ratio of the length of the crank 52 to the link 53 is 1:4 to 1:6. The stirring frequency of the stirring motor 51 is selected to be 0 to 20 Hz. However, according to the test results of various samples, it is considered that the stirring frequency is in the range of 2 to 8 Hz, which can achieve the best stirring effect and save the nucleic acid purification time.

As shown in FIG. 9, the stirring sleeve driving mechanism 5 and the magnetic bar lifting mechanism 6 are both mounted on the support plate 12, the stirring sleeve driving mechanism 5 is located below the magnetic bar lifting mechanism 6, and the stirring sleeve 10 is located below the magnetic bar frame 11. The magnetic rod frame 11 and the stirring sleeve 10 are located directly above the deep orifice plate 22 on the table 14. For safety and aesthetics, the shutter 16 mounted on the support plate 12 separates the agitator frame 10 and the magnetic bar frame 11 at the front of the base 13 from the other components at the rear. The magnetic bar lifting mechanism 6, the magnetic bar frame 11, the stirring sleeve driving mechanism 5, the stirring jacket 10, the support plate 12, and the shielding plate 16 collectively constitute a bracket device 9.

As shown in FIG. 3, FIG. 4 and FIG. 5, the bracket lifting device 8 is composed of a bracket lifting motor 81, a bracket lifting motor mounting plate 82, a bracket lifting screw 83, a bracket lifting screw nut 84, and an intermediate bracket 85. The support plate drive member 86, the bracket lifting slider 87, the bracket lifting rail 88, and the bent sheet 89 are formed. The carriage lift motor 81 is attached to the upper portion of the intermediate bracket 85 via the bracket lift motor mounting plate 82. The bracket lifting screw 83 is fixedly coupled to the bracket lifting motor 81 (or the bracket lifting screw 83 is integrated with the bracket lifting motor 81 shaft). The bracket lift screw nut 84 and the support plate drive member 86 are integrally fixed by bolts. As shown in FIG. 5, the support plate driving member 86 is fastened to the side of the support plate 12 in the bracket device 9, and is fastened. The carriage lifting motor 81 drives the carriage lifting screw nut 84 up and down by the carrier lifting screw 83, and the carrier lifting screw nut 84 then drives the carriage device 9 up and down through the support plate 12. A bracket lifting slider 87 is attached to the front surface of the support plate 12 of the bracket device 9, and the bracket lifting slider 87 is slidably coupled to the bracket lifting rail 88 fastened to the intermediate bracket 85 so that the bracket device 9 can be Stabilizing smoothly up and down, during the lifting process of the bracket device 9, the magnetic bar 20 is inserted into the bottom of the stirring sleeve 21, and the magnetic bar 20 and the stirring sleeve 21 are moved up and down, that is, the e action shown in FIG. The action sucks the magnetic beads into and into the deep holes of the deep well plate 22. The bent sheet 89 fixed to the support plate driving member 86 transmits a position signal to the control system 2 via the photoelectric position sensor 57 fixed to the intermediate holder 85, which in turn controls the carriage lifting motor 81. The bracket lifting device 8 of the present invention realizes that the magnetic bar 20 and the stirring sleeve 21 are combined (the magnetic rod 20 is inserted into the bottom of the stirring sleeve 21), and then moved up and down together, and the bracket lifting motor 81 is independently driven, the displacement is convenient to control, and the movement is performed. The process of magnetic beads is safe and reliable.

The screw nut mechanism composed of the bracket lifting screw 83 and the bracket lifting screw nut 84 may be a sliding screw nut mechanism, a ball screw nut mechanism, a timing belt reciprocating mechanism, a rack and pinion mechanism, and the like.

As shown in FIG. 3, the power source of the carriage translating device 7 is a carriage translation motor 71. The carriage translation motor 71 is fixed on the base 13 by the bracket translation motor mounting plate 72. The carriage translation motor 71 drives the carriage translation screw 73 to rotate, and the carriage translation screw 73 drives the carriage to translate the screw nut 74 horizontally. mobile. One side of the drive bending plate 75 is fixedly coupled to the bracket translation screw nut 74, and the other side of the bending is bolted to the intermediate bracket 85 in the bracket lifting mechanism. The carriage translation slider 76 is mounted on the intermediate bracket 85, and the carriage translation guide 77 is fixed to the base 13. The carriage translation slider 76 is slidably coupled to the carriage translation rail 77 in a horizontal direction, and the carriage translation screw nut 74 drives the intermediate bracket 85 to horizontally move along the carriage translation guide 77 by the drive bending plate 75. At this time, the carriage lifting device 8 where the intermediate bracket 85 is located and the bracket device 9 are not moved in the horizontal direction, and the two are integrated. Therefore, the driving curved plate 75 drives the bracket device 9 to move horizontally. During the horizontal movement of the bracket device 9, the magnetic bar 20 is inserted into the bottom of the stirring sleeve 21, and the horizontal movement of the magnetic bar 20 and the stirring sleeve 21 is the same as that of FIG. The d action shown. The d action cooperates with the a and e actions described above to complete the handling of the magnetic beads. The horizontal movement of the carriage device 9 is smoother due to the arrangement of the carriage translation slider 76 and the carriage translation guide 77. The bent sheet 2 78 fixed to the drive bending plate 75 cooperates with the photoelectric position sensor 57 to realize position signal transmission of the horizontal movement of the carriage device 9.

The screw nut mechanism composed of the bracket translation screw 73 and the bracket translation screw nut 74 may be a sliding screw nut mechanism, a ball screw nut mechanism, a timing belt reciprocating mechanism, a rack and pinion mechanism, and the like. .

Further, in a state where the magnetic bar 20 is not inserted into the agitating sleeve 21, the cradle lifting device 8 and the cradle translating device 7 can also drive the cradle device 9 to perform lifting and translation to complete some auxiliary actions of the nucleic acid purification process.

The slider and the rail mechanism in the stirring sleeve driving mechanism 5, the magnetic rod lifting mechanism 6, the bracket translation device 7, and the bracket lifting device 8 are rolling linear guide mechanisms, and the friction between the guide rail and the slider is rolling friction The resistance is smaller and the movement of the moving parts is smoother and smoother.

As described above, the magnetic bar lifting and lowering mechanism 6 in the cradle device 9 realizes an operation (a operation) in which the magnetic bar 20 is separately raised and lowered, and the magnetic bar 20 is inserted or taken out of the agitating sleeve 21. The bracket lifting device 8 and the bracket translation device 7 respectively realize the up and down movement and translation movement (d action and e action) after the magnetic bar 20 and the stirring sleeve 21 are combined, and the a, d, and e actions are combined to complete the magnetic bead handling work. . The stirring sleeve driving mechanism 5 can drive the stirring sleeve 21 to move up and down separately and perform stirring (b action and c action), and complete the mixing operation of the stirring sleeve 21 into and out of the deep hole and the magnetic beads and the liquid in the deep hole. The control system 2 sequentially controls the operation of each motor based on the signal of the photoelectric position sensor 57 to complete the nucleic acid purification process.

The stirring sleeve driving mechanism 5, the magnetic rod lifting mechanism 6, the bracket translation device 7, and the bracket lifting device 8 are four independently controllable and movable components, and can complete various actions and action sequences by changing the program of the control system. Combine to realize the nucleic acid purification process to meet the individual needs of users.

While various embodiments of the present invention have been shown and described, it will be understood by those skilled in the art The scope of the invention is defined by the claims and their equivalents.

Claims

A nucleic acid purifying apparatus comprising a mechanical part (1), a control system (2), an operating unit (3), a casing (4), the mechanical part (1) is located in a lower part of the nucleic acid purifying apparatus, and the control system (2) And the operating unit (3) is located at the upper part of the instrument, the outer casing (4) is wrapped around the outermost part of the instrument, and the mechanical part (1) comprises a stirring sleeve driving mechanism (5), a stirring sleeve (10), a magnetic rod lifting Mechanism (6), magnetic rod frame (11), bracket or support plate (12), base (13), table (14), table translation device or bracket translation device, said stirring frame (10) is installed On the stirring sleeve driving mechanism (5), the magnetic rod frame (11) is mounted on the magnetic rod lifting mechanism (6), the stirring sleeve driving mechanism (5) and the magnetic rod lifting mechanism ( 6) mounted on a bracket or support plate (12), the bracket or support plate (12) and the work table (14) are mounted on the base (13), the work table (14) is disposed on the a magnetic bar frame (11) and the stirring frame (10) below;

It is characterized in that

The stirring sleeve driving mechanism (5) comprises a stirring motor (51), a crank (52), a connecting rod (53), a stirring sleeve slider (54), a stirring sleeve rail (55), and the stirring motor (51) shaft Fixedly connected to the crank (52), the crank (52) is rotatably connected to one end of the connecting rod (53), and the other end of the connecting rod (53) is rotatable with the stirring sleeve slider (54) Connected, the stirring sleeve (10) is fixed on the stirring sleeve slider (54), and the stirring sleeve slider (54) is slidably connected with the stirring sleeve rail (55).
The nucleic acid purifying apparatus according to claim 1, further comprising a cradle lifting device (8) comprising a cradle lifting motor (81), an intermediate bracket (85), and a holder a lifting screw (83), a bracket lifting screw nut (84), a bracket lifting slider (87), a bracket lifting rail (88), and the bracket lifting motor (81) is mounted on the intermediate bracket (85) The bracket lifting screw (83) is pivotally coupled to the bracket lifting motor (81), and the bracket lifting screw nut (84) is fitted to the bracket lifting screw (83) The bracket lifting screw nut (84) is coupled to the support plate (12), and the bracket lifting slider (87) is mounted on the support plate (12) A rail (88) is fixed to the intermediate bracket (85), and the bracket lifting slider (87) is slidably coupled to the bracket lifting rail (88).
The nucleic acid purifying apparatus according to claim 2, wherein said holder translation means is a carriage translation means (7), said carriage translation means (7) comprising a carriage translation motor (71), carriage translation a lead screw (73), a carriage translation screw nut (74), a carriage translation slider (76), a carriage translation guide rail (77), and the bracket translation motor (71) is mounted to the base (13) The bracket translation screw (73) is pivotally coupled to the carriage translation motor (71), and the carriage translation screw nut (74) is fitted over the carriage translation screw (73) The bracket translation screw nut (74) is coupled to the bracket lifting device (8), and the bracket translation slider (76) is mounted on the bracket lifting device (8) A carriage translation rail (77) is mounted on the base (13), the bracket translation slider (76) being slidably coupled to the carriage translation rail (77).
The nucleic acid purifying apparatus according to claim 1, wherein said magnetic bar lifting mechanism (6) comprises a magnetic bar lifting motor (61), a magnetic bar lifting screw (62), and a magnetic bar lifting screw nut (63). a magnetic bar lifting slider (64), a magnetic bar lifting rail, the magnetic bar lifting motor (61) shaft is connected to the magnetic bar lifting screw (62), the magnetic bar lifting screw nut (63) Suited on the magnetic bar lifting screw (62), the magnetic bar lifting screw nut (63) is connected to the magnetic bar frame (11), and the magnetic bar lifting slider (64) is fixed on the On the magnetic bar frame (11), the magnetic bar lifting slider (64) is slidably coupled to the magnetic bar lifting rail.
The nucleic acid purifying apparatus according to claim 1, wherein the stirring sleeve driving mechanism (5) further comprises a flap (56), a position sensor (57), a bearing (58), and the flap (56) Fixed to the crank (52), the position sensor (57) is fixed beside the crank (52), the baffle (56) is a thin plate, the thickness direction of which passes through the slot on the position sensor (57), The bearing (58) is mounted within the bore of the connecting rod (53).
The nucleic acid purifier according to claim 5, wherein the flap (56) has a right-angled sector shape.
The nucleic acid purifying apparatus according to any one of claims 1 to 4, wherein the slider and the rail mechanism are both ball linear guide mechanisms.
The nucleic acid purifying apparatus according to claim 1, wherein the crank (52) and the link (53) of the stirring sleeve driving mechanism (5) are made of an aluminum alloy.
The nucleic acid purifying apparatus according to claim 1, wherein a ratio of a length of the crank (52) to the connecting rod (53) is less than 1:1, and a frequency of the stirring motor (51) is 0 to 20 Hz.
The nucleic acid purifying apparatus according to claim 1, wherein a ratio of a length of the crank (52) to the connecting rod (53) is 1:4 to 1:6, and a frequency of the stirring motor (51) is 2. ~8Hz.