CN216106983U

CN216106983U - Nucleic acid extraction device

Info

Publication number: CN216106983U
Application number: CN202022844595.2U
Authority: CN
Inventors: 王乔明; 黄林山; 柯华滔
Original assignee: Guangzhou Bay Area Biotechnology Co ltd
Current assignee: Guangzhou Bay Area Biotechnology Co ltd
Priority date: 2020-12-01
Filing date: 2020-12-01
Publication date: 2022-03-22
Anticipated expiration: 2030-12-01

Abstract

The utility model discloses a nucleic acid extraction device, which is provided with a base, a stirring mechanism, an X-axis driving assembly and a tray mechanism, wherein the stirring mechanism comprises a frame fixed on the base, and a magnetic rod assembly, a magnetic sleeve frame and a vibration assembly which are arranged on the frame; moreover, tray mechanism includes tray and the Y axle drive assembly who is connected with the tray drive, and the tray can be close to or keep away from rabbling mechanism under Y axle drive assembly's drive, realizes the transform of position, has made things convenient for operating personnel to get the sample on the operation panel and has put, and the efficiency that the nucleic acid drawed is effectively improved.

Description

Nucleic acid extraction device

Technical Field

The utility model relates to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction device.

Background

The extraction of nucleic acid from a biological sample is a key premise for accurately completing nucleic acid detection, and in the prior art, a magnetic bead method is one of relatively mature nucleic acid extraction methods, but the existing device for extracting nucleic acid by using the magnetic bead method is complex in structure, inconvenient to operate and low in nucleic acid extraction efficiency.

SUMMERY OF THE UTILITY MODEL

The present invention has been made to solve at least one of the problems of the prior art, and an object of the present invention is to provide a nucleic acid isolation apparatus.

The nucleic acid extraction device according to an embodiment of the present invention includes a base; the stirring mechanism is provided with a frame fixed on the base, a magnetic rod assembly, a magnetic sleeve frame and a vibration assembly, the magnetic rod assembly, the magnetic sleeve frame and the vibration assembly are installed on the frame, the magnetic sleeve frame is located below the magnetic rod assembly, and the vibration assembly drives the magnetic sleeve frame and the magnetic rod assembly to reciprocate in the vertical direction; the X-axis driving assembly is arranged on the base and is connected with the stirring mechanism; and the tray mechanism comprises a tray and a Y-axis driving component in driving connection with the tray.

According to the nucleic acid extraction device of the embodiment of the utility model, at least the following beneficial effects are achieved: the nucleic acid extraction device is provided with a base, a stirring mechanism, an X-axis driving assembly and a tray mechanism, wherein the stirring mechanism comprises a frame fixed on the base, and a magnetic rod assembly, a magnetic sleeve frame and a vibration assembly which are arranged on the frame, wherein the vibration assembly drives the magnetic sleeve frame and the magnetic rod assembly to reciprocate along the vertical direction so as to realize the rapid stirring of sample liquid; moreover, tray mechanism includes tray and the Y axle drive assembly who is connected with the tray drive, and the tray can be close to or keep away from rabbling mechanism under Y axle drive assembly's drive, realizes the transform of position, has made things convenient for operating personnel to get the sample on the operation panel and has put, and the efficiency that the nucleic acid drawed is effectively improved.

According to some embodiments of the present invention, the Y-axis driving assembly includes a pair of guide rails fixed on the base and a belt transmission mechanism for driving the tray to move, the two guide rails are distributed on two sides of the frame in parallel, and the tray is overlapped on the two guide rails.

According to some embodiments of the utility model, the belt transmission mechanism comprises a pair of synchronizing wheels, a timing belt wound around the two synchronizing wheels, and a tray motor in driving connection with one of the synchronizing wheels, wherein the timing belt is connected with the tray.

According to some embodiments of the present invention, the magnetic rod assembly includes a magnetic rod rack, a plurality of magnetic rods fixed on the magnetic rod rack, and a stepping motor installed on the top of the frame, a mounting plate is installed on the front side of the frame, a first vertical guide rail for slidably mounting the magnetic rod rack is installed on the mounting plate, and the stepping motor drives the magnetic rod rack to slide along the first vertical guide rail.

According to some embodiments of the utility model, at least one limiting rod fixed on the magnetic sleeve frame is arranged between the magnetic rod frame and the magnetic sleeve frame, and each limiting rod is vertically arranged and the top end of each limiting rod penetrates through the magnetic rod frame.

According to some embodiments of the utility model, a linear bearing is respectively sleeved on the periphery of the top end of each limiting rod, and the lower end of each linear bearing is fixedly connected to the magnetic rod rack.

According to some embodiments of the present invention, the vibration assembly includes a vibration motor disposed on a back side of the frame and a ball screw drivingly connected to the vibration motor, a screw of the ball screw is vertically disposed, at least one side of the ball screw is provided with a second vertical guide rail, the second vertical guide rail is provided with a first slider and a second slider, the first slider is connected to the mounting plate, and the second slider is fixed to the magnetic sleeve holder.

According to some embodiments of the utility model, the vibration motor is a servo motor, and the servo motor is connected with the screw of the ball screw through a coupling.

According to some embodiments of the utility model, the X-axis drive assembly is provided with a lead screw motor, an output shaft of which is connected to the frame.

According to some embodiments of the utility model, the base is provided with an X-axis slide rail, an X-axis slide block is slidably mounted on the X-axis slide rail, an output shaft of the lead screw motor is connected with the X-axis slide block, and the frame is fixed with the X-axis slide block.

According to some embodiments of the utility model, four rows of the magnetic rods are arranged on the magnetic rod rack in parallel, each row comprises two groups of the magnetic rods, and the number of the magnetic rods in each group is 12.

Drawings

The utility model is further described below with reference to the accompanying drawings and examples;

FIG. 1 is a schematic diagram of a nucleic acid isolation apparatus according to an embodiment of the present invention;

FIG. 2 is a diagram showing the construction of the nucleic acid isolation apparatus according to another aspect of the embodiment of the present invention.

Detailed Description

Reference will now be made in detail to the present preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout.

In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.

Referring to fig. 1 and 2, the nucleic acid extraction device according to the embodiment of the present invention includes a base 1, a stirring mechanism 2, an X-axis driving assembly, and a tray mechanism, wherein the stirring mechanism 2 includes a frame 21 fixed on the base 1, and a magnetic rod assembly 22, a magnetic sleeve holder 23, and a vibration assembly mounted on the frame 21, the magnetic sleeve holder 23 is located below the magnetic rod assembly 22, and the vibration assembly drives the magnetic sleeve holder 23 and the magnetic rod assembly 22 to perform a reciprocating motion in a vertical direction; the X-axis driving assembly is arranged on the base 1 and is connected with the stirring mechanism 2; the tray mechanism includes a tray (not shown) and a Y-axis drive assembly drivingly connected to the tray.

The nucleic acid extraction device provided by the embodiment of the utility model comprises a base 1, a stirring mechanism 2, an X-axis driving assembly and a tray mechanism, wherein the stirring mechanism 2 comprises a frame 21 fixed on the base 1, a magnetic rod assembly 22, a magnetic sleeve frame 23 and a vibration assembly, the magnetic rod assembly 22, the magnetic sleeve frame 23 and the vibration assembly are installed on the frame 21, and the vibration assembly drives the magnetic sleeve frame 23 and the magnetic rod assembly 22 to reciprocate along the vertical direction so as to realize rapid stirring of sample liquid; moreover, tray mechanism includes tray and the Y axle drive assembly who is connected with the tray drive, and the tray can be close to or keep away from rabbling mechanism 2 under Y axle drive assembly's drive, realizes the transform of position, has made things convenient for operating personnel to get the sample on the operation panel and has put, and the efficiency of nucleic acid extraction is effectively improved.

In some embodiments, the Y-axis driving assembly includes a pair of guide rails 411 fixed on the base 1 and a belt transmission mechanism 412 driving the tray to move, the two guide rails 411 are distributed on two sides of the frame 21 in parallel, the tray is overlapped on the two guide rails 411, the arrangement of the guide rails 411 limits the sliding direction of the tray, can avoid the situation that the tray moves and deviates, and ensures that the tray can smoothly slide out to the front end of the base 1 under driving. Further, as shown in fig. 1, in this embodiment, the belt transmission mechanism 412 includes a pair of synchronizing wheels, a timing belt wound around the two synchronizing wheels, and a tray motor in driving connection with one of the synchronizing wheels, and the timing belt is connected to the tray. The belt transmission mechanism 412 is used for driving the tray to move in and out on the base 1, so that the movement of the tray can be kept smooth, the generated noise is low, and the silent operation of the tray is ensured; the arrangement of the double guide rails 411 also ensures that the tray can keep parallel movement in the movement process, and prevents the tray from twisting and deviating and the like.

As shown in fig. 1, the magnetic rod assembly 22 includes a magnetic rod rack 221, a plurality of magnetic rods 222 fixed on the magnetic rod rack 221, and a stepping motor 223 mounted on the top of the frame 21, further, a mounting plate is disposed on the front side of the frame 21, a first vertical guide rail 211 for slidably mounting the magnetic rod rack 221 is disposed on the mounting plate, and the stepping motor drives the magnetic rod rack 221 to slide along the first vertical guide rail. The magnetic sleeve frame 23 is provided with magnetic sleeves 231 with the same number as the magnetic rods 22, and each magnetic sleeve 231 and the corresponding magnetic rod 22 are respectively positioned on the same vertical line. Under the driving of the stepping motor, the magnetic rod 22 can fall into or fall off the magnetic sleeve 231, so as to realize magnetic attraction or release. In order to ensure that each magnetic rod 22 can smoothly fall into the corresponding magnetic sleeve 231, a limit structure is arranged between the magnetic rod rack 221 and the magnetic sleeve rack 23 to prevent the magnetic rod rack 221 from horizontally moving relative to the magnetic sleeve rack 23; specifically, in this embodiment, the position limiting structure has two position limiting rods 251 fixed on the magnetic sleeve frame 23, each position limiting rod 251 is vertically disposed, and the top end of each position limiting rod 251 passes through the magnetic rod frame 221, wherein the top end periphery of each position limiting rod 251 is further sleeved with a linear bearing 252, and the lower end of each linear bearing 252 is fixedly connected to the magnetic rod frame 221. It is understood that in other embodiments, the number of the limiting rods 251 may be one or more than two.

As shown in fig. 2, the vibration assembly of this embodiment includes a vibration motor 241 disposed on the back side of the frame 21 and a ball screw 242 drivingly connected to the vibration motor 241, wherein a screw of the ball screw 242 is vertically disposed, the frame 21 on both sides of the ball screw 242 is respectively provided with a second vertical guide rail 212, the second vertical guide rail 212 is provided with a first slider 2121 and a second slider 2122 connected to a nut of the ball screw 242, further, the first slider 2121 is connected to a mounting plate, and the second slider 2122 is fixed to the magnetic frame 23. When the vibration motor 241 is started, the nut of the ball screw 242 will displace along the screw, so as to drive the first slider 2121 and the second slider 2122 to slide up and down along the second vertical guide rail 212, and further draw the magnetic rod frame 221 and the magnetic sleeve frame 23 to realize up-and-down reciprocating motion, thereby achieving the stirring effect. More specifically, the vibration motor 241 is a servo motor, and the servo motor is connected to a screw of the ball screw 242 through a coupling 243.

More specifically, the X-axis driving assembly is provided with a lead screw motor 31, an output shaft of the lead screw motor 31 is connected to the frame 21, the lead screw motor 31 is started, and the frame 21 linearly moves along with the output shaft of the lead screw motor 31, so as to achieve the effect of adjusting the position of the stirring mechanism 2 in the X-axis direction. Referring to fig. 1, the base 1 is provided with an X-axis slide rail 32, an X-axis slide block 33 is slidably mounted on the X-axis slide rail 32, wherein an output shaft of the lead screw motor 31 is connected to the X-axis slide block 33, and the frame 21 is fixed to the X-axis slide block 33.

In some embodiments, referring to fig. 1, four rows of magnetic rods 22 are arranged in parallel on the magnetic rod rack 221, each row includes two sets of magnetic rods 22, and the number of each set of magnetic rods 22 is 12, i.e. the total number of magnetic rods 22 mounted on the magnetic rod rack 221 is 96. Therefore, when the nucleic acid extraction device is used for carrying out nucleic acid extraction operation, 96 samples can be extracted together at one time, and the efficiency of nucleic acid extraction is greatly improved.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention.

Claims

1. A nucleic acid extraction device, characterized in that: comprises that

A base;

the stirring mechanism is provided with a frame fixed on the base, a magnetic rod assembly, a magnetic sleeve frame and a vibration assembly, the magnetic rod assembly, the magnetic sleeve frame and the vibration assembly are installed on the frame, the magnetic sleeve frame is located below the magnetic rod assembly, and the vibration assembly drives the magnetic sleeve frame and the magnetic rod assembly to reciprocate in the vertical direction;

the X-axis driving assembly is arranged on the base and is connected with the stirring mechanism; and

the tray mechanism comprises a tray and a Y-axis driving assembly in driving connection with the tray.

2. The nucleic acid extraction apparatus according to claim 1, characterized in that: the Y-axis driving assembly comprises a pair of guide rails fixed on the base and a belt transmission mechanism driving the tray to move, the two guide rails are distributed on two sides of the frame in parallel, and the tray is overlapped on the two guide rails.

3. The nucleic acid extraction apparatus according to claim 2, characterized in that: the belt transmission mechanism comprises a pair of synchronous wheels, a synchronous belt wound on the two synchronous wheels and a tray motor in driving connection with one synchronous wheel, and the synchronous belt is connected with the tray.

4. The nucleic acid extraction apparatus according to claim 1, characterized in that: the bar magnet subassembly includes bar magnet frame, a plurality of fixing bar magnet on the bar magnet frame and installs the step motor at the top of frame, the front side of frame is equipped with the mounting panel, set up the confession on the mounting panel bar magnet frame slidable mounting's a vertical guide rail, step motor drive the bar magnet frame is followed first vertical guide rail slides.

5. The nucleic acid extraction apparatus according to claim 4, characterized in that: at least one limiting rod fixed on the magnetic sleeve frame is arranged between the magnetic rod frame and the magnetic sleeve frame, each limiting rod is vertically arranged, and the top end of each limiting rod penetrates through the magnetic rod frame.

6. The nucleic acid extraction apparatus according to claim 5, characterized in that: the top end periphery of each limiting rod is sleeved with a linear bearing, and the lower end of each linear bearing is fixedly connected to the magnetic rod rack.

7. The nucleic acid extraction apparatus according to claim 4, characterized in that: the vibration subassembly is including locating the dorsal part of frame vibrating motor and with the ball that the vibrating motor drive is connected, the vertical setting of screw rod of ball, at least one side of ball is equipped with the vertical guide rail of second, install on the vertical guide rail of second and connect the first slider and the second slider of ball's nut, first slider is connected the mounting panel, just the second slider with the magnetic sleeve frame is fixed.

8. The nucleic acid extraction apparatus according to claim 7, characterized in that: the vibration motor is a servo motor, and the servo motor is connected with a screw rod of the ball screw through a coupler.

9. The nucleic acid extraction apparatus according to claim 1, characterized in that: the X-axis driving assembly is provided with a lead screw motor, and an output shaft of the lead screw motor is connected with the frame.

10. The nucleic acid extraction apparatus according to any one of claims 4 to 8, characterized in that: four rows of magnetic rods are arranged on the magnetic rod rack in parallel, each row comprises two groups of magnetic rods, and the number of the magnetic rods in each group is 12.