CN215404279U - Nucleic acid processing device - Google Patents
Nucleic acid processing device Download PDFInfo
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- CN215404279U CN215404279U CN202120723164.2U CN202120723164U CN215404279U CN 215404279 U CN215404279 U CN 215404279U CN 202120723164 U CN202120723164 U CN 202120723164U CN 215404279 U CN215404279 U CN 215404279U
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Abstract
The present invention specifically discloses a nucleic acid processing apparatus, including: the device comprises a rack, a slide rail is arranged at the upper part of the rack, and an amplification device is arranged at the lower part of the rack; the sample adding device comprises a suction head extracting device and an injection device which is used for matching with the suction head extracting device to suck a reagent and inject the reagent; a drive device; the sample adding device is connected with the sliding rail in a sliding mode, and the driving device is matched with the sample adding device and used for driving the sample adding device to slide on the sliding rail. The structure is simple and clear, and the operation is centralized, thereby avoiding cross contamination.
Description
Technical Field
The utility model relates to the field of biological sample detection and analysis, in particular to a nucleic acid processing device.
Background
In order to realize the detection and analysis of nucleic acid (including deoxyribonucleic acid DNR and ribonucleic acid RNA) in a biological sample, the nucleic acid needs to be extracted through processes of oscillation, magnetic attraction, elution and the like in the middle, then the quantity of the nucleic acid is amplified through heating incubation, and finally the nucleic acid is detected and analyzed through fluorescence detection, wherein each process needs a specific device for carrying out the detection and analysis, reagents (or samples) need to be sucked and injected, so that various reagents react with the sample, a new pollution-free suction head needs to be replaced each time to suck and inject the reagents (or samples), the positions of the suction head, the suction reagent (or samples) and the injection reagent (or samples) are different, the actions are different, and different mechanisms are needed to complete, so that the parts related to the reagent sucking and the reagent injection in the current nucleic acid processing device have complicated structures and are stacked by parts, on one hand, the operation steps are complicated, on the other hand, cross contamination is easy to cause.
SUMMERY OF THE UTILITY MODEL
In view of the above, there is a need for a nucleic acid processing apparatus with a simple and clear structure and centralized operation, thereby avoiding cross contamination.
In order to achieve the purpose, the utility model adopts the following technical scheme:
a nucleic acid processing apparatus comprising: the device comprises a rack, a slide rail is arranged at the upper part of the rack, and an amplification device is arranged at the lower part of the rack; the sample adding device comprises a suction head extracting device and an injection device which is used for matching with the suction head extracting device to extract a reagent and inject the reagent; a drive device; the sample adding device is connected with the sliding rail in a sliding mode, and the driving device is matched with the sample adding device and used for driving the sample adding device to slide on the sliding rail.
Further, drive arrangement includes motor, take-up pulley, hold-in range, the motor is equipped with the action wheel, the action wheel with the take-up pulley tensioning hold-in range, the application of sample device with the hold-in range is connected.
Further, the frame is equipped with two the slide rail, the application of sample device still includes the slide suction head extraction element with injection apparatus install in the slide, slide and two slide rail sliding fit.
Furthermore, the top of frame is equipped with the hoist and mount window, two the slide rail is located respectively the both sides of hoist and mount window, two the slide rail is located the top surface of frame, suction head extraction element with injection device pass through the hoist and mount window install in the slide below.
Further, the slide is arranged on the slide rail in a erecting mode.
Further, drive arrangement includes motor, take-up pulley, hold-in range, the motor is equipped with the action wheel, the action wheel with the take-up pulley tensioning the hold-in range, the slide with the hold-in range is connected.
Further, the motor is arranged at the rear end of the rack and is positioned below the synchronous belt.
Further, the hold-in range is located the slide below, the hold-in range is located two between the slide rail.
Further, the application of sample device still includes the mounting bracket, the mounting bracket includes bottom plate, left side board, right side board, suction head extraction element with injection device install in the bottom plate, left side board and right side board are located respectively the both sides of bottom plate, the top of left side board with the top of right side board install in the slide, the hold-in range is located the left side board with between the right side board.
Further, the application of sample device is still including taking off the suction head device, take off the suction head device including taking off the suction head board and being used for the drive take off suction head actuating mechanism of suction head board reciprocating motion, suction head extraction element includes that the suction head draws the needle and is used for the drive the suction head draws the suction head actuating mechanism of needle reciprocating motion, it is equipped with the suction head hole to take off the suction head board, the position in suction head hole and the position that the suction head drawn the needle correspond, it is used for supplying to take off the suction head hole the washing head draws the needle and passes and draw the suction head, the width in suction head hole is less than by the suction head draws the width of the suction head that the needle extracted.
The utility model has the beneficial effects that:
set up the slide rail in the frame, through the gliding mode of guide rail guide, the removal of control application of sample device realizes the accurate displacement of application of sample device, and the application of sample device includes suction head extraction element, injection apparatus, and suction head extraction element is used for changing the suction head, and the amplification device is used for loading sample test tube, reagent consumptive material, and injection apparatus is used for absorbing and injecting reagent (this paper the reagent includes detect reagent, sample and other materials that need be used in nucleic acid detection), and the application of sample device sets up in frame upper portion, and the amplification device sets up in the frame lower part, and the two is independent mutually noninterfere relatively, and the clear mutually noninterfere of operation, application of sample device moving state can accurate control, avoids cross contamination. Simultaneously, the application of sample device moves through gliding mode, especially when the application of sample device is equipped with a plurality of application of sample passageways along the vertical direction of its self direction of motion, the motion orbit mutually noninterfere of each application of sample passageway has avoided the motion orbit of an application of sample passageway to span to the condition of another application of sample passageway motion orbit, prevents the cross contamination between the application of sample passageway.
Drawings
FIG. 1 is a schematic view showing the construction of a nucleic acid processing apparatus according to the present invention;
FIG. 2 is an exploded view of the nucleic acid processing apparatus of the present invention;
FIG. 3 is a schematic view of a partial structure of the frame and the driving device;
FIG. 4 is a schematic view of the structure of the sample adding device;
FIG. 5 is an exploded view of a sample application device;
FIG. 6 is a schematic view of the structure of an extraction tip of the sample application device;
FIG. 7 is a schematic view of the structure of a sample-removing tip of the sample-adding device;
fig. 8 is a schematic structural view of the sample adding device sliding on the slide rail.
Reference numerals:
1. the device comprises a rack, 11, a slide rail, 12, a hoisting window, 2, a sample adding device, 21, a suction head removing device, 211, a suction head removing plate, 212, a suction head removing driving mechanism, 213, a suction head removing hole, 22, a slide seat, 23, a mounting rack, 231, a bottom plate, 232, a left side plate, 233, a right side plate, 24, a suction head extracting device, 241, a suction head extracting needle, 242, a suction head driving mechanism, 25, an injection device, 3, a driving device, 31, a motor, 311, a driving wheel, 32, a synchronous belt, 33, a tension wheel, 4, an amplification device, 41, a test tube rack, 42, an incubation magnetic suction module, 43, a fluorescence detection module, 5 and a suction head.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be further clearly and completely described below with reference to the embodiments of the present invention. It should be noted that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", and the like, are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus are not to be construed as limiting the present invention.
The terms "first," "second," "third," "fourth," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, the definitions of "first", "second", "third", "fourth" features may explicitly or implicitly include one or more of such features.
As shown in FIG. 1, the nucleic acid processing apparatus 4 of the present invention comprises: the device comprises a frame 1, a sample adding device 2 arranged on the upper part of the frame 1, a driving device 3 arranged on the frame 1 and used for driving the sample adding device 2, and an amplification device 4 arranged on the lower part of the frame 1.
As shown in FIGS. 2 to 5, the sample adding device 2 comprises a tip extracting device 24, an injecting device 25 for injecting and extracting reagents (the reagents described herein include detection reagents, samples, and other materials required for nucleic acid detection) in cooperation with the tip extracting device 24, and a tip removing device 21. A tip extraction device 24 is used to extract the tip 5. The injection device 25 is used to withdraw and inject reagents. The tip removal device 21 is used to remove the tip 5 from the tip extraction device 24. The sample adding device 2 is slidably connected to the slide rail 11, and the driving device 3 is matched with the sample adding device 2 to drive the sample adding device 2 to slide on the slide rail 11, for example, to a position shown in fig. 8. Through the gliding mode of slide rail 11 guide, control application of sample device 2's removal realizes application of sample device 2's accurate displacement.
The amplification device 4 comprises a test tube rack 41, an incubation magnetic attraction module 42 and a fluorescence detection module 43. The test tube rack 41 is used for loading sample test tubes, the incubation magnetic suction module 42 is used for loading consumables and performing incubation and magnetic suction, and the fluorescence detection module 43 is used for nucleic acid detection.
The upper portion of frame 1 is equipped with two slide rails 11, and two slide rails 11 are located the top surface of frame 1, and the top of frame 1 is equipped with hoist and mount window 12, and two slide rails 11 are located the both sides of hoist and mount window 12 respectively, and suction head extraction element 24, injection device 25, the device 21 of taking off the suction head are installed in slide 22 below through hoist and mount window 12. Specifically, the sample adding device 2 further comprises a slide carriage 22, and the suction head extracting device 24, the injection device 25 and the suction head removing device 21 are arranged below the slide carriage 22. The slide 22 is mounted on the slide rail 11, and the slide 22 is slidably engaged with the two slide rails 11. Make slide 22 atress balanced through setting up two slides 22, slide 22 is retrained by two slide rails 11, keeps slide 22 to be in the translation state at the removal in-process, avoids slide 22 spin to the position of accurate control application of sample device 2, absorption and injection reagent (or sample) that can be accurate.
The driving device 3 comprises a motor 31, a tension pulley 33 and a synchronous belt 32, the motor 31 is provided with a driving wheel 311, the driving wheel 311 and the tension pulley 33 are installed on the top surface of the frame 1, the driving wheel 311 and the tension pulley 33 tension the synchronous belt 32, the sliding seat 22 is connected with the synchronous belt 32, and the sliding seat 22 can be driven to move horizontally by the rotation of the motor 31. The slide carriage 22 drives the suction head extraction device 24 and the injection device 25 to move, so that the weight of the suction head extraction device 24 and the injection device 25 is evenly distributed to the slide rail 11 through the slide carriage 22, and the whole movement is more stable.
The slide rail 11 is arranged on the top surface of the frame 1, and the driving wheel 311, the tension wheel 33 and the synchronous belt 32 are also arranged on the top surface of the frame 1, which is equivalent to the driving device 3 being externally arranged outside the frame 1, thereby facilitating the maintenance. Because slide rail 11 locates the top surface of frame 1, slide 22 erects again on slide rail 11, locates hold-in range 32 between left side board 232 and right side board 233 to make hold-in range 32 be in the slide 22 below, hold-in range 32 also is located between two slide rails 11 simultaneously, utilizes the distance between slide 22 and the top surface of frame 1 to put hold-in range 32 ingeniously, can save space better. In addition, the suction head extracting device 24, the injection device 25 and the suction head removing device 21 are mounted on the slide carriage 22 through the mounting frame 23, the synchronous belt 32 can be accommodated in the space between the left side plate 232 and the right side plate 233 of the mounting frame 23, and on the other hand, the weight of the sample adding device 2 is uniformly transmitted to the slide carriage 22 through the left side plate 232 and the right side plate 233, so that the skew is not easy to occur, and the motion state and the stop position of the sample adding device 2 can be controlled more accurately.
According to the utility model, the rotation of the motor 31 is converted into linear motion by adopting a synchronous belt 32 transmission mode, so that the torque of the motor 31 is stably output, and the smooth translation of the sample adding device 2 is realized by matching with the guide of the slide rail 11, so that the shock of pause and contusion is avoided, the sucked liquid is prevented from falling down, and the cross contamination is avoided.
The motor 31 is arranged at the rear end of the frame 1, and the motor 31 is positioned below the synchronous belt 32. Accomodate motor 31 ingenious in frame 1 rear end, compact structure practices thrift the space, especially, only keeps action wheel 311 and puts motor 31 upside down in frame 1 above frame 1 for the thickness of whole frame 1 top reduces by a wide margin.
As shown in fig. 4 and 5, the suction head 5 removing device comprises a suction head 5 removing plate 211 and a suction head driving mechanism 242212 for driving the suction head 5 removing plate 211 to reciprocate, the suction head extracting device 24 comprises a suction head extracting needle 241 and a suction head driving mechanism 242 for driving the suction head extracting needle 241 to reciprocate, the suction head 5 removing plate 211 is provided with a suction head removing hole 213, the position of the suction head removing hole 213 corresponds to the position of the suction head extracting needle 241, the suction head removing hole 213 is used for allowing the suction head 5 extracting needle to pass through the suction head 5, the width of the suction head removing hole 213 is smaller than the width of the suction head 5 extracted by the suction head extracting needle 241, and the width means that the suction head 5 cannot pass through the suction head removing hole 213, so that the suction head 5 is pushed by the suction head 5 removing plate 211 to remove the suction head 5 from the suction head extracting needle 241.
As shown in FIGS. 6 to 8, when the tip 5 needs to be extracted, the driving device 3 drives the sample adding device 2 to slide above the tip 5, the tip driving mechanism 242 drives the tip extracting needle 241 to move downward, the tip extracting needle 241 is inserted into the tip 5, and the tip extracting needle 241 extracts the tip 5 by interference fit, as shown in FIG. 6.
After the suction head 5 is extracted, the injection device 25 is communicated with the suction head 5 through the suction head extraction needle 241, and the injection device 25 can reduce the pressure in the suction head 5 to suck the reagent into the suction head 5 or increase the pressure in the suction head 5 to inject the reagent outwards. The driving device 3 and the tip driving mechanism 242 may act together to move the tip 5, for example, the driving device 3 and the tip driving mechanism 242 insert the tip 5 into the reagent, the injection device 25 reduces the pressure inside the tip 5, the reagent is aspirated into the tip 5, the driving device 3 and the tip driving mechanism 242 further move the tip 5 above the next vessel to be operated, the injection device 25 increases the pressure inside the tip 5, and the reagent is squeezed out of the tip 5 and injected into a predetermined vessel, thereby completing the transfer of the reagent by the tip 5. To avoid contamination, reagents are only contacted with the pipette tip 5 and not with the injection device 25, one pipette tip 5 can only be used for one reagent, and after the operation is completed, the pipette tip 5 needs to be replaced to aspirate and inject another reagent. When the suction head 5 needs to be replaced, the suction head removing driving mechanism 212 drives the suction head removing plate 211 to move downwards relative to the suction head extracting needle 241, the suction head 5 is clamped by the suction head removing hole 213 because the width of the suction head removing hole 213 is smaller than that of the suction head 5 extracted by the suction head extracting needle 241, the suction head 5 is pushed by the suction head removing plate 5 211 to move downwards relative to the suction head extracting needle 241, finally the suction head 5 is pushed by the suction head removing plate 211 to withdraw from the suction head extracting needle 241, so that the suction head 5 is separated from the suction head extracting needle 241, as shown in fig. 7, after the suction head 5 is separated from the suction head extracting needle 241, the operation can be repeated, the suction head 5 is extracted again, and the reagent is transferred by the suction head 5.
This embodiment sets up slide rail 11 on frame 1, and through the gliding mode of slide rail 11 guide, the removal of control application of sample device 2 realizes the accurate displacement of application of sample device 2. The sample adding device 2 is arranged on the upper portion of the rack 1, the amplification device 4 is arranged on the lower portion of the rack 1, the two devices are independent and do not interfere with each other, the operation is clear and does not interfere with each other, the moving state of the sample adding device 2 can be accurately controlled, and cross contamination is avoided. Meanwhile, as shown in fig. 1 and 6, the sample adding device 2 is provided with 8 sample adding channels along the vertical direction of the movement direction of the sample adding device, the 8 sample adding channels are arranged side by side, and can simultaneously extract 8 suction heads 5, the sample adding device 2 slides along the slide rail 11, the movement tracks of the sample adding channels are not interfered with each other, the situation that the movement track of one sample adding channel stretches across the movement track of the other sample adding channel is avoided, and the cross contamination between the sample adding channels is prevented.
In the present invention, the synchronous belt 32 is adopted to convert the rotation of the motor 31 into the translation to drive the sample adding device 2 to slide, but is not limited to this embodiment, and other mechanisms can also be adopted to drive the sample adding device 2 to slide on the slide rail 11, for example, in another embodiment, the motor can be adopted to drive the screw rod to drive the sample adding device 2 to slide on the slide rail.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A nucleic acid processing apparatus, comprising:
the device comprises a rack, a slide rail is arranged at the upper part of the rack, and an amplification device is arranged at the lower part of the rack;
the sample adding device comprises a suction head extracting device and an injection device which is used for matching with the suction head extracting device to suck a reagent and inject the reagent;
a drive device;
the sample adding device is connected with the sliding rail in a sliding mode, and the driving device is matched with the sample adding device and used for driving the sample adding device to slide on the sliding rail.
2. The nucleic acid processing device according to claim 1, wherein the driving means comprises a motor, a tension pulley, and a timing belt, the motor is provided with a driving pulley, the driving pulley and the tension pulley tension the timing belt, and the sample adding means is connected to the timing belt.
3. The apparatus for processing nucleic acid according to claim 1, wherein the rack has two slide rails, and the sample injection device further comprises a slide carriage, the tip extraction device and the injection device are mounted on the slide carriage, and the slide carriage is slidably engaged with the two slide rails.
4. The apparatus according to claim 3, wherein a lifting window is provided at the top of the rack, two of the slide rails are respectively provided at two sides of the lifting window, the two slide rails are provided at the top surface of the rack, and the tip extracting device and the injecting device are installed below the slide base through the lifting window.
5. The nucleic acid processing apparatus according to claim 4, wherein the carriage is mounted on the slide rail.
6. The nucleic acid processing apparatus according to claim 4, wherein the driving means comprises a motor, a tension pulley, and a timing belt, the motor is provided with a driving pulley, the driving pulley and the tension pulley tension the timing belt, and the carriage is connected to the timing belt.
7. The nucleic acid processing apparatus according to claim 6, wherein the motor is provided at a rear end of the rack, and the motor is located below the timing belt.
8. The nucleic acid processing device according to claim 6, wherein the timing belt is located below the carriage, and the timing belt is located between the two slide rails.
9. The nucleic acid processing device according to claim 6, wherein the sample adding device further comprises a mounting rack, the mounting rack comprises a bottom plate, a left side plate and a right side plate, the tip extracting device and the injecting device are mounted on the bottom plate, the left side plate and the right side plate are respectively disposed on two sides of the bottom plate, the top of the left side plate and the top of the right side plate are mounted on the slide base, and the synchronous belt is located between the left side plate and the right side plate.
10. The nucleic acid processing device according to any one of claims 1 to 9, wherein the sample application device further comprises a tip removal device, the tip removal device comprises a tip removal head plate and a tip removal driving mechanism for driving the tip removal head plate to reciprocate, the tip extraction device comprises a tip extraction needle and a tip driving mechanism for driving the tip extraction needle to reciprocate, the tip removal head plate is provided with a tip removal hole, the position of the tip removal hole corresponds to the position of the tip extraction needle, the tip removal hole is used for allowing the tip extraction needle to pass through an extraction tip, and the width of the tip removal hole is smaller than the width of a tip extracted by the tip extraction needle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120723164.2U CN215404279U (en) | 2021-04-09 | 2021-04-09 | Nucleic acid processing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120723164.2U CN215404279U (en) | 2021-04-09 | 2021-04-09 | Nucleic acid processing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215404279U true CN215404279U (en) | 2022-01-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120723164.2U Active CN215404279U (en) | 2021-04-09 | 2021-04-09 | Nucleic acid processing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215404279U (en) |
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2021
- 2021-04-09 CN CN202120723164.2U patent/CN215404279U/en active Active
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