CN113088445A - Full-automatic nucleic acid amplification detection analyzer - Google Patents
Full-automatic nucleic acid amplification detection analyzer Download PDFInfo
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- CN113088445A CN113088445A CN202110382181.9A CN202110382181A CN113088445A CN 113088445 A CN113088445 A CN 113088445A CN 202110382181 A CN202110382181 A CN 202110382181A CN 113088445 A CN113088445 A CN 113088445A
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- 238000001514 detection method Methods 0.000 title claims abstract description 88
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 59
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 59
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 59
- 230000003321 amplification Effects 0.000 title claims abstract description 52
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 52
- 238000000605 extraction Methods 0.000 claims abstract description 165
- 230000007246 mechanism Effects 0.000 claims abstract description 62
- 230000010355 oscillation Effects 0.000 claims abstract description 54
- 238000002347 injection Methods 0.000 claims abstract description 29
- 239000007924 injection Substances 0.000 claims abstract description 29
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 20
- 238000011534 incubation Methods 0.000 claims abstract description 19
- 238000011068 loading method Methods 0.000 claims abstract description 7
- 230000001360 synchronised effect Effects 0.000 claims description 29
- 238000001914 filtration Methods 0.000 claims description 12
- 229920000742 Cotton Polymers 0.000 claims description 5
- 238000012864 cross contamination Methods 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 49
- 238000003795 desorption Methods 0.000 description 34
- 238000013519 translation Methods 0.000 description 26
- 238000010438 heat treatment Methods 0.000 description 22
- 239000000463 material Substances 0.000 description 19
- 239000003153 chemical reaction reagent Substances 0.000 description 16
- 230000005389 magnetism Effects 0.000 description 11
- 230000005284 excitation Effects 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
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- 108020004414 DNA Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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Abstract
The invention particularly discloses a full-automatic nucleic acid amplification detection analyzer, which comprises a machine shell with an opening on one side, a cabin door arranged at the opening of the machine shell, a nucleic acid extraction and amplification device horizontally and vertically arranged at the bottom of the machine shell towards the cabin door, a multi-channel sample adding device arranged above the nucleic acid extraction and amplification device and connected to the upper end of the machine shell in a sliding manner, and an industrial control module; the nucleic acid extraction and amplification device comprises a sliding frame which can be sent out or retracted along a cabin door and a sending-out driving device; a sample carrier, an incubation module, an extraction carrier for loading extraction consumables, a magnetic attraction device arranged below the extraction carrier and a fluorescence detection device with oscillation are sequentially arranged on the sliding frame from the cabin door inwards; the multi-channel sample adding device comprises an extraction head for extracting a suction head, an injection assembly, an extraction driving mechanism, a suction head removing plate and a suction head removing driving mechanism, wherein the injection assembly is connected with the extraction head and is communicated with the extraction head, the extraction driving mechanism is used for driving the extraction head to move up and down, and the suction head removing driving mechanism is used for driving the suction head removing plate to move up and down. The invention realizes the full-automatic operation of nucleic acid extraction, amplification and detection, effectively reduces the detection cost, avoids cross contamination and improves the detection accuracy.
Description
Technical Field
The invention relates to the field of biological sample detection and analysis, in particular to a full-automatic nucleic acid amplification detection analyzer.
Background
The extraction detection analysis of nucleic acid (including deoxyribonucleic acid DNR and ribonucleic acid RNA) in a biological sample needs a plurality of procedures of extraction and purification (cracking-combining-shaking-magnetic attraction-washing-elution) -amplification-detection analysis and the like. At present, devices for nucleic acid extraction and purification, nucleic acid amplification reaction and nucleic acid detection are separated, and the whole detection is carried out manually or linked, so that the detection time is consumed, and the detection efficiency is low. Meanwhile, the labor cost and the probability of errors possibly caused by human factors are increased, and the pollution risk is greatly increased due to the turnover of consumables among different devices.
Further, chinese patent document CN112300911A discloses a nucleic acid detecting instrument including a mounting rack, a reagent plate, a cover plate unit, a heat treatment unit, a pipetting device, an oscillation unit, and a magnetic attraction unit. Realizes the integration of nucleic acid extraction and amplification detection. However, since the devices have complicated structures and the parts are mixed and piled up, and all the equipment is in an open environment, on one hand, the operation steps are complicated, and on the other hand, cross contamination is easily caused.
Disclosure of Invention
In view of the above, there is a need to provide a fully automatic nucleic acid detecting analyzer with simple and convenient operation, which is integrated in a closed detection environment, and can effectively improve the detection efficiency, reduce the detection cost, avoid cross contamination, and improve the detection accuracy.
In order to achieve the purpose, the invention adopts the following technical scheme:
a full-automatic nucleic acid amplification detection analyzer comprises a machine shell with an opening on one side, a cabin door arranged at the opening of the machine shell, a nucleic acid extraction and amplification device horizontally and vertically arranged at the bottom of the machine shell towards the cabin door, a multi-channel sample adding device arranged above the nucleic acid extraction and amplification device and connected to the upper end of the machine shell in a sliding manner, and an industrial control module;
the nucleic acid extraction and amplification device comprises a sliding frame which can be sent out or retracted along the hatch door and a sending-out driving device; a sample carrier, an incubation module, an extraction carrier for loading extraction consumables, a magnetic attraction device arranged below the extraction carrier and a fluorescence detection device with oscillation are sequentially arranged on the sliding frame from the cabin door inwards;
the multichannel sample adding device comprises an extraction head for extracting a suction head, an injection assembly, an extraction driving mechanism, a suction head removing plate and a suction head removing driving mechanism, wherein the injection assembly is connected with the extraction head and is communicated with the extraction head, the extraction driving mechanism is used for driving the extraction head to move up and down, and the suction head removing driving mechanism is used for driving the suction head removing plate to move up and down.
Furthermore, the machine shell comprises a rack, a top plate, a bottom plate and side plates, wherein the top plate and the bottom plate are respectively fixed at the upper end part and the lower end part of the rack; the lower end part of the cabin door is rotatably pivoted on the bottom plate at the opening of the shell, and a torsional spring is sleeved at the pivoting part of the cabin door and the bottom plate.
Furthermore, the sliding frame is provided with a collision wheel at the end part close to the cabin door, and the collision wheel is used for pushing the cabin door to turn outwards and open when the sliding frame is sent out.
Further, be equipped with the slide rail on the roof of casing one end of slide rail is equipped with the action wheel that the motor drove another tip of slide rail be equipped with from the driving wheel, the tensioning in the action wheel and from the hold-in range between the driving wheel, the top of multichannel application of sample device is equipped with the slide, the slide passes through the hold-in range and drives slide around on the slide rail.
Furthermore, the nucleic acid extraction and amplification device further comprises a support frame, a limiting block is arranged on the bottom plate, the support frame is installed on the bottom plate through the limiting block, the sliding frame is arranged on the support frame, and the support frame is matched with the delivery driving device to deliver or withdraw the sliding frame from or into the cabin door.
Further, the sending-out driving device comprises a sending-out motor, a sending-out driving wheel, a sending-out synchronous belt and two sending-out driven wheels, wherein the sending-out driving wheel is arranged on an output shaft of the sending-out motor, the two sending-out driven wheels are arranged below the driving wheel, two ends of the sending-out synchronous belt are fixed on the supporting frame, and the two sending-out driven wheels compress the sending-out synchronous belt on the sending-out driving wheel.
Further, the fluorescence detection device with oscillation comprises a fluorescence detection module, an oscillation mechanism arranged above the fluorescence detection module, a detection consumable carrier arranged on the oscillation mechanism, and an oscillation driving mechanism for driving the detection consumable carrier to move back and forth and left and right on the oscillation mechanism along the horizontal direction.
The air filtering device further comprises an air filtering module, wherein the air filtering module comprises a lower cover, an upper cover, filter cotton arranged in the lower cover, an air outlet arranged on the upper cover and an exhaust fan arranged at the lower end of the air outlet; the lower cover is installed above the top plate.
Furthermore, the industrial control module comprises a touch screen industrial personal computer and an installation cross beam, the touch screen industrial personal computer is installed on the cross beam, and the cross beam is installed above the cabin door on the rack.
And the sample scanning module is arranged on the rack between the cabin door and the industrial control module.
The invention has the beneficial effects that:
through with nucleic acid extraction amplification device and multichannel application of sample device integration in the confined testing environment that casing and hatch door formed, through the process of whole nucleic acid's extraction, amplification and detection of industrial control module control, realize the full automatic operation of nucleic acid extraction, amplification and detection, and avoided cross contamination, improve the detection accuracy rate when effectively having reduced detection cost. Meanwhile, the nucleic acid extraction and amplification device is wholly sent out or retracted into the cabin door under the driving of the sliding frame, so that test consumables can be conveniently placed and replaced, and cleaning and maintenance are also convenient. In addition, the multi-channel sample adding device can effectively ensure that samples or reagents only contact with the suction head in the process of extraction and injection, and the suction head can be automatically replaced with a new suction head after the suction head is used up, thereby effectively avoiding the mutual cross contamination of the injection assembly, the suction head, the samples or reagents and different samples or reagents.
Drawings
FIG. 1 is a schematic diagram of a full-automatic nucleic acid amplification detection analyzer according to the present invention;
FIG. 2 is a schematic diagram of a fully automatic nucleic acid amplification detection analyzer with parts removed;
FIG. 3 is an exploded view of a fully automatic nucleic acid amplification detection analyzer with parts removed;
FIG. 4 is a schematic view of the nucleic acid extraction and amplification apparatus;
FIG. 5 is an exploded view of the nucleic acid extracting and amplifying apparatus;
FIG. 6 is a schematic view showing a structure of a nucleic acid extracting and amplifying apparatus with parts removed;
FIG. 7 is a schematic structural diagram of an incubation module;
FIG. 8 is a schematic view of a translational type magnetic attraction structure;
FIG. 9 is a schematic view of a magnetic attraction state of the translation magnetic attraction structure;
FIG. 10 is a schematic view of a rotary magnetic attraction structure;
FIG. 11 is an exploded view of the rotary magnetic attraction structure;
FIG. 12 is a schematic view of a magnetic attraction position of the rotary magnetic attraction structure;
FIG. 13 is a schematic view of a fluorescence detection device with oscillation;
FIG. 14 is an exploded view of a fluorescence detection device with oscillation;
FIG. 15 is a schematic structural view of an oscillation driving eccentric shaft;
FIG. 16 is a schematic view of the structure of a multi-channel sample adding device;
FIG. 17 is an exploded view of a multi-channel sample application device;
FIG. 18 is a schematic structural view of a driving structure of the suction head;
FIG. 19 is a schematic view of the injection assembly;
FIG. 20 is a schematic view of the top of the housing with parts partially removed;
FIG. 21 is a schematic diagram of a process of extracting a tip from a multi-channel sample adding device;
FIG. 22 is a schematic view of the pipetting process of the multi-channel sample application device;
FIG. 23 is a schematic view of the loading process of the multi-channel loading device;
FIG. 24 is a schematic view of the process of removing the suction head;
FIG. 25 is a schematic diagram of a process for extracting a tube cap from a multi-channel sample adding device;
FIG. 26 is a schematic diagram of a capping process of the multi-channel sample adding device;
FIG. 27 is a schematic view of an air filtration module configuration;
FIG. 28 is a schematic view of a sample scanning configuration;
fig. 29 is a schematic structural diagram of an ultraviolet sterilization lamp.
Reference numerals:
1-a machine shell, 11-a rack, 12-a bottom plate, 13-a top plate, 14-a side plate, 15-a limiting block, 16-a sliding rail, 17-driving wheels 17, 18-driven wheels and 19-a synchronous belt;
2-hatch door, 21-torsion spring;
3-nucleic acid extraction and amplification device, 31-support frame, 312-pressing plate, 313-connecting rod, 32-sliding frame, 321-collision block, 33-sending drive device, 331-sending motor, 332-sending driving wheel, 333-sending synchronous belt, 34-sample carrier, 341-test tube, 35-incubation module, 351-heating block, 352-heating film,
353-incubation support, 354-thermal baffle, 36-extraction carrier, 361-extraction consumables, 37-magnetic attraction device,
371-translation guide rail assembly, 3711-translation track, 3712-translation sliding block, 372-translation magnetic attraction assembly, 3721-magnet, 373-translation driving assembly, 3731-translation stepping motor, 3732-translation synchronous belt, 3733-translation synchronous wheel, 374-rotation rotating shaft, 375-magnet frame, 3722-first magnet, 3723-second magnet, 376-rotation driving assembly, 3761-rotation driving motor, 3762-rotation synchronous belt, 3763-rotation driving wheel, 3764-rotation driven wheel, 38-fluorescence detection device with oscillation, 381-fluorescence detection module, 382-oscillation mechanism, 383-detection consumable carrier, 384-oscillation driving mechanism, 3811-detection support frame, 3812-detection module driving mechanism, 3813-detection module support, 3814-detection module guide bar, 3815-detection module slide, 3816-detection module drive, 3817-detection module wire track, 3821-oscillation support plate, 3822-first oscillation guide bar, 3823-first oscillation slide, 3824-second oscillation guide bar, 3825-second oscillation slide, 3841-oscillation drive link, 3842-oscillation drive eccentric shaft, 3843-oscillation drive motor, 3844-main shaft portion, 3845-eccentric shaft portion,
39-crash wheel, 391-handle;
4-multichannel loading device, 41-extraction head, 42-injection component, 421-injection tube, 422-piston, 423-injection driving device, 43-extraction driving mechanism, 431-extraction slide rail, 432-extraction slide carriage, 433-extraction motor, 434-extraction synchronous belt, 435-extraction screw rod, 436-base, 4331-extraction driving wheel, 4351-extraction driven wheel, 44-desorption head plate, 45-desorption head driving mechanism, 451-desorption head slide carriage, 452-desorption head bracket, 453-desorption head motor, 454-desorption head synchronous belt, 455-desorption head screw rod, 4531-desorption head driving wheel, 4551-desorption head driving wheel and 46-suction head; 5-industrial control module, 51-touch screen industrial personal computer, 52-installation beam;
6-air filtration module, 61-lower cover, 62-upper cover, 63-filter cotton, 64-air outlet, 65-exhaust fan; 7-sample scanning module, 71-scanner, 72-ultraviolet sterilizing lamp, 73-status indicator lamp; 8-tube cover.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be further clearly and completely described below with reference to the embodiments of the present invention. It should be noted that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", and the like, are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus are not to be construed as limiting the present invention.
The terms "first," "second," "third," "fourth," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, the definitions of "first", "second", "third", "fourth" features may explicitly or implicitly include one or more of such features.
As shown in fig. 1-3, the automatic nucleic acid amplification detection analyzer of the present invention comprises a casing 1 with an opening at one side, a hatch 2 disposed at the opening of the casing, a nucleic acid extraction amplification device 3 disposed at the bottom of the casing and vertically and horizontally facing the hatch 2, a multi-channel sample feeding device 4 disposed above the nucleic acid extraction amplification device and slidably connected to the upper end of the casing 1, and an industrial control module 5.
The casing 1 includes a frame 11, a top plate 13 and a bottom plate 12 fixed on the upper and lower end portions of the frame, and side plates 14 surrounding the three side surfaces of the frame 11, and a limiting block 15 is arranged on the upper bottom surface of the bottom plate 12.
The lower end of the hatch 2 is rotatably pivoted to the bottom plate 12 of the casing through a rotating shaft, specifically, the lower end of the hatch 2 and the side of the bottom plate 12 are respectively provided with a shaft hole, and a rotating shaft is inserted into the shaft holes, or the lower end of the hatch 2 is provided with a rotating shaft, and the side of the bottom plate 12 is provided with a shaft hole matched with the rotating shaft, which is not limited to the listed modes, but also can be other modes capable of realizing rotation. Meanwhile, as shown in fig. 3, a torsion spring 21 is installed at the pivot joint of the hatch door 2 and the bottom plate 12, and the hatch door can be automatically closed under the action of the torsion spring 21.
It should be noted that the hatch 2 is independently configured in accordance with the number of the nucleic acid extraction and amplification devices, and the number of the hatch and the number of the nucleic acid extraction and amplification devices in the drawing of this embodiment are 3 respectively, so that the hatch can be opened only according to the corresponding hatch of the sent nucleic acid extraction and amplification device, thereby avoiding the influence on the detection and analysis of other nucleic acid extraction and amplification detection devices and reducing the possibility of contamination.
The nucleic acid extracting and amplifying device 3, as shown in fig. 4-6, includes a support frame 31, a carriage 32 disposed on the support frame 31 and capable of being sent out or retracted along the hatch 2, a sending-out driving device 33, and a sample carrier 34, an incubation module 35, an extraction carrier 36 for loading extraction consumables, a magnetism attracting device 37 disposed under the extraction carrier, and a fluorescence detecting device 38 with oscillation, which are disposed on the carriage 32 in sequence from the hatch 2, wherein the nucleic acid extracting and amplifying device 3 is mounted on the bottom plate 12 by being clamped on the limit block 15.
As shown in fig. 6, the feeding drive device 33 includes a feeding motor 331, a feeding drive pulley 332, a feeding timing belt 333, and two feeding driven pulleys (not shown) disposed on an output shaft of the feeding motor 331, the two feeding driven pulleys being disposed below the feeding drive pulley 332, both ends of the feeding timing belt 333 being fixed to the support frame 31, and the two feeding driven pulleys pressing the feeding timing belt 333 against the feeding drive pulley 333. The industrial control module 5 is electrically connected to the sending-out motor 331, and sends the carriage 32 and the sample carrier 34 placed on the carriage 32, the incubation module 35, the extraction carrier 36 loaded with the extraction consumables, the magnetic attraction device 37 placed below the extraction carrier, and the fluorescence detection device 38 with oscillation out of the housing 1 by driving the sending-out motor 331 to place and replace the test consumables, and then drives the sending-out motor to withdraw the carriage into the housing after placing or replacing the test consumables. Meanwhile, the cleaning and maintenance are convenient.
Preferably, as shown in fig. 6, the supporting frame 31 is further provided with a pressing plate 311, one end of the pressing plate 311 is pivotally connected to a side wall of the supporting frame 31, the pressing plate 311 is pivotally connected to a side wall of the supporting frame 31 near the other end, and a foldable connecting rod 313 is hinged to the side wall of the supporting frame 31, a collision block 321 is provided on a side wall of the sliding frame 32 corresponding to the supporting frame 31, and the collision block 321 is used for pushing the foldable connecting rod 313 to open when the sliding frame 32 is sent out, and pressing the foldable connecting rod 313 to fold when the sliding frame 32 is retracted, so. And then compress tightly the consumptive material on drawing the carrier through the clamp plate, do benefit to the heating and hatch and can prevent that the consumptive material from shifting. Preferably, a resilient member is disposed at the pivot joint of the pressing plate 311 and the supporting frame 31, and the pressing plate 311 can be automatically opened or closed under the resilient force of the resilient member. Preferably, the carriage 32 is provided with a striker 39 at the end close to the hatch, the striker 39 pushing the hatch 2 to flip open outwards when the carriage is delivered. Preferably, a handle is arranged between the pair of collision wheels 39, so that an operator can conveniently hold the handle to send out or retract the sliding frame.
The sample carrier 34 is used to place or hold the test tube 341 to be tested.
The incubation module 35 is used for heating, controlling temperature and incubating the consumables 361 in the extraction carrier. As shown in fig. 7, incubation module 35 includes heating block 351 provided with incubation hole, attached heating film 352 of a lateral wall of heating block 351, fixed heating block 351 and incubation support 353 of heating film 352, heating block 351's incubation hole is used for inserting the consumptive material 361 that the splendid attire extracted the consumptive material, heating film 352 and industrial control module 5 electric connection, after the consumptive material 361 that extracts the consumptive material inserts heating block 351's incubation hole, industrial control module 5 control heating film 352 generates heat, heat and incubate for extracting the consumptive material through heating block 352's conduction. Heating block 351 is last to be seted up a plurality of holes of incubating, can incubate a plurality of consumptive materials of extracting simultaneously. Preferably, heat shields 354 are provided on the other side of the heating film 352 to reduce or eliminate the need for heating consumables near the heating film.
The extraction carrier 36 is used for placing and fixing the extraction consumables 361.
The magnetic attracting device 37 is used for attracting magnetism of the consumable 361 in the incubation module 35.
The magnetic attraction mode of the magnetic attraction device 37 is a translational magnetic attraction structure, as shown in fig. 8-9, the translational magnetic attraction structure includes a translational guide rail component 371, a translational magnetic attraction component 372 and a translational driving component 373, the translational guide rail component 371 includes a translational track 3711 and a translational slider 3712 in sliding fit with the translational track 3711, the translational magnetic attraction component 372 includes a magnet 3721, and the translational driving component 373 is butted with the translational slider 3712; wherein, the magnetic assembly 372 is installed on the sliding block 3712, and the driving assembly 373 is electrically connected to the industrial control module 5 for driving the magnetic assembly 372 to slide on the sliding track 3711. Realize the translation of translation magnetism subassembly 372 through translation guide rail subassembly 371, when needs inhale the magnetism, inhale the subassembly 372 with the translation magnetism and remove to the front end and let magnet 3721 be close to the consumptive material 361 of hatching module 35 and inhale the magnetism (as shown in fig. 9), when stopping inhaling the magnetism, inhale the subassembly 372 with the translation magnetism and withdraw to the rear and let magnet 3721 keep away from consumptive material 361. Translation guide rail subassembly 371 adopts translation slider 3712 and translation track 3711's sliding fit, makes whole device remove steadily, does benefit to the control and inhales the position of magnetism to the adsorption state of accurate control magnetic bead improves and draws purity. And the structure is small and the occupied space is small. Preferably, the translation driving assembly 373 includes a translation stepping motor 3731, a translation synchronous belt 3732 and a translation synchronous wheel 3733, and the translation stepping motor drives the translation synchronous belt to drive the translation synchronous wheel to drive the translation sliding block 3712 to slide, so as to control the displacement of the translation magnetic attraction assembly more accurately.
The magnetic attraction device 37 is a rotary magnetic attraction structure, as shown in fig. 10-12, the rotary magnetic attraction structure includes a rotary shaft 374, at least one magnet frame 375 mounted on the rotary shaft 374, and a first magnet 3722 and a second magnet 3723 mounted at two ends of the magnet frame 375, the first magnet 3722 and the second magnet 3723 respectively extend out of two sides of the rotary shaft 374, the magnet frame 375 and the rotary shaft 374 constitute a structure similar to a "seesaw", the first magnet 3722 and the second magnet 3723 are located at two ends of the "seesaw", the rotary driving component 376 drives the rotary shaft 374 to rotate and switch between a first position and a second position, when the rotary shaft 374 rotates to the first position, the first magnet 3722 is located at a first magnetic attraction station, and at this time, the first magnet 3722 is close to the bottom surface of the incubation hole (i.e. the first magnet 3722 is close to the bottom surface of the extraction consumable material 361) (as shown in fig. 12 a), when the rotating shaft 374 rotates to the second position, the second magnet 3723 is located at the second magnetic attraction station, and the second magnet 3723 is close to the side surface of the incubation hole (i.e. the second magnet 3723 is close to the side surface of the consumable part 361) (see fig. 12 b). The rotation type is inhaled the magnetic structure and can be freely switched different and inhale the magnetism position, and then the magnetic bead that controls effectively in the extraction consumptive material is concentrated, satisfies the extraction consumptive material volume of different size volumes and shifts. Preferably, the rotary driving assembly 376 includes a rotary driving motor 3761 and a rotary timing belt 3762, a rotary driving wheel 3763 provided at an output shaft of the rotary driving motor, a rotary driven wheel 3764 installed at an end portion of the rotary shaft 374, and the rotary driving wheel 3763 and the rotary driven wheel 3764 tension the rotary timing belt 3762. When the rotary driving motor 3761 rotates, the rotary driving wheel 3763 is driven to rotate, and further the rotary rotating shaft 374 is driven to rotate through the rotary synchronous belt 3762 and the rotary driven wheel 3764, so that the switching of the magnetic attraction position is realized. The rotary driving motor 3761 is electrically connected to the industrial control module 5, and the industrial control module controls the rotation of the rotary driving motor. Preferably, the rotation driving motor 3761 is a stepping motor, which can control the rotation angle of the rotation shaft 374 more precisely, and further control the positions of the first magnet 3722 and the second magnet 3723.
The fluorescence detecting apparatus 38 with oscillation, as shown in fig. 13-14, includes a fluorescence detecting module 381, an oscillation mechanism 382 disposed above the fluorescence detecting module, a detecting consumable carrier 383 disposed on the oscillation mechanism, and an oscillation driving mechanism 384 for driving the detecting consumable carrier 383 to move back and forth and left and right in a horizontal direction on the oscillation mechanism 382.
The fluorescence detection module 381 includes at least one excitation light source set (not shown), at least one fluorescence light-gathering set (not shown), at least one photodiode (not shown), and a detection support frame 3811, the detection support frame 3811 is provided with an excitation light source hole and a fluorescence detection hole, the excitation light source set is disposed below the excitation light source hole, the excitation light source set includes an LED light source, an excitation light source light-gathering lens set and a filter, the fluorescence light-gathering set is disposed below the fluorescence detection hole, and the fluorescence light-gathering set includes a fluorescence light-gathering lens set and a second filter.
Preferably, the fluorescence detection module 381 is further provided with a detection module driving mechanism 3812, the detection module driving mechanism 3812 includes a pair of detection module supports 3813 arranged at intervals, a pair of detection module guide rods 3814 arranged between the pair of detection module supports 3813 in parallel, a detection module sliding seat 3815 slidably sleeved on the pair of detection module guide rods 3814, and a detection module driving device 3816 driving the detection module sliding seat 3815, the detection module wire rail 3817 and the pair of detection module guide rods 3814 are arranged on the detection module sliding seat 3815 in a horizontal direction perpendicular to the horizontal direction, and the detection support 3811 of the fluorescence detection module 381 is movably arranged on the detection module wire rail 3817 through the detection module driving device 3816. Preferably, the sensing module driver 3816 is a stepper motor, which can determine the exact position within the range of travel of the sensing module. The movement of the fluorescence detection module 381 in the horizontal plane in the horizontal and vertical directions is enabled by the detection module driving mechanism 3812, so that the detection of a plurality of consumables on the detection consumable carrier can be realized.
The oscillation mechanism 382 includes, as shown in fig. 14, a pair of oscillation support plates 3821 oppositely disposed at both sides of the fluorescence detection module 381, a pair of first oscillation guide rods 3822 fixed between both ends of the upper end portions of the pair of oscillation support plates 3821, a first oscillation slide 3823 slidably fitted over the pair of first oscillation guide rods 3822, a pair of second oscillation guide rods 3824 horizontally and vertically fixed to the first oscillation slide 3823, and a second oscillation slide 3825 slidably fitted over the pair of second oscillation guide rods 3824.
The oscillation driving mechanism 384, as shown in fig. 14, includes an oscillation driving connecting rod 3841, an oscillation driving eccentric shaft 3842 and an oscillation driving motor 3843, the oscillation driving eccentric shaft 3842 includes a main shaft portion 3844 and an eccentric shaft portion 3845 (as shown in fig. 15) provided at one end of the main shaft portion 3844 and offset with respect to the axis of the main shaft portion 3844, the main shaft portion 3844 of the oscillation driving eccentric shaft 3842 is vertically rotatably mounted on the shaft bracket through a bearing, the oscillation driving motor 3843 is vertically fixed through a motor bracket, the output end of the oscillation driving motor 3843 is connected with the lower end of the main shaft portion 3844 of the oscillation driving eccentric shaft 3842 through a timing belt, one end of the oscillation driving connecting rod 3841 is sleeved on the eccentric shaft portion 3845 at the upper end of the oscillation driving eccentric shaft 3842, and the other end of the.
The oscillation driving motor 3843 drives the oscillation driving eccentric shaft 3842 to rotate, the eccentric shaft part 3845 at the upper end of the oscillation driving eccentric shaft 382 makes a circular motion around the axis of the main shaft part 3844, a circular motion track taking the axis of the main shaft part as a circle center is formed in an overlooking manner, the oscillation driving connecting rod 3841 drives the detection consumable carrier 383 and the detection consumable to move on the oscillation mechanism 382 along the motion track of the eccentric shaft part 3845, and a reagent in the detection consumable carries out eddy current rotation along the inner wall of the detection consumable tube. Detect reagent in the consumptive material on the consumptive material carrier and carry out rotary motion along detecting the consumptive material inside pipe wall, realize the vortex misce bene, improve amplification efficiency, and then guaranteed the accuracy of testing result.
The multi-channel sample adding device 4, as shown in the figure, includes an extraction head 41 for extracting a suction head 46, an injection assembly 42 connected to the extraction head 41 and communicating with each other, an extraction driving mechanism 43 for driving the extraction head 41 to move up and down, a desorption head plate 44, and a desorption head driving mechanism 45 for driving the desorption head plate to move up and down.
The head removing plate 44 is provided with a head removing hole 441, wherein the position of the head removing hole 441 corresponds to the position of the extraction head 41, the head removing hole 441 is used for allowing the extraction head 41 to pass through the extraction head 46, and the aperture of the head removing hole 441 is smaller than the aperture of the extraction head 46 extracted by the extraction head 41.
The extraction driving mechanism 43 comprises an extraction sliding rail 431 and an extraction sliding seat 432 connected with the extraction sliding rail 431 in a sliding manner, an extraction motor 433, an extraction synchronous belt 434 and an extraction screw 435, wherein the extraction motor 433 is provided with an extraction driving wheel 4331, the extraction screw 435 is provided with an extraction driven wheel 4351 linked with the extraction screw, the extraction driving wheel 4331 and the extraction driven wheel 4351 tension the extraction synchronous belt 434, the extraction sliding seat 432 is provided with an extraction screw hole, the extraction screw 435 is in threaded fit with the extraction screw hole, the extraction sliding seat 432 is provided with a base 436, an extraction head 41 is arranged on the base 436, the extraction motor 433 is electrically connected with the industrial control module 5, the industrial control module 5 drives the extraction motor 433 to rotate to drive the extraction screw 435 to rotate, the rotation of the extraction motor 433 is converted into the up-down movement of the extraction sliding seat 432 through the threaded fit of the extraction screw 435 and the extraction screw hole, and (6) pipetting. Preferably, the extraction motor 433 and the extraction screw 435 are located on the same side of the extraction timing belt 434, whereby a space above the extraction screw 435 can be saved.
The suction head removing driving mechanism 45 comprises a suction head removing slide seat 451, a suction head removing support 452 connected with the suction head removing slide seat 451, a suction head removing motor 453, a suction head removing synchronous belt 454 and a suction head removing screw 455, wherein the suction head removing motor 453 is provided with a suction head removing driving wheel 4531, the suction head removing screw 455 is provided with a suction head removing driving wheel 4551 linked with the suction head removing screw, the suction head removing driving wheel 4531 and the suction head removing driving wheel 4551 tension the suction head removing synchronous belt 454, the support 452 is provided with a suction head removing screw hole, the suction head removing screw 455 is in threaded fit with the suction head removing screw hole, a suction head removing plate 44 is installed between two wings of the suction head removing support, the suction head removing slide seat 432 is simultaneously in sliding fit with the base 436, and the suction head removing motor 453 is electrically connected with the industrial control module 5. When the industrial control module 5 drives the suction head removing motor 453 to rotate, the rotation of the suction head removing motor 453 is converted into the up-and-down movement of the suction head removing bracket 452 through the threaded matching of the suction head removing screw rod 455 and the suction head removing screw hole, and then the suction head removing plate 44 is driven to move up and down relative to the extraction head 41, so that the suction head 46 is separated from the extraction head 41 by the suction head removing plate 44. Preferably, the suction head removing motor 453 and the suction head removing screw 455 are located on the same side of the suction head timing belt 454, so that the space above the suction head removing screw 455 is saved.
The injection assembly 42, as shown in the figure, comprises a syringe 421, a piston 422 sliding in the syringe 421, and an injection driving device 423 connected with the piston 422, the extraction head 41 is connected with the syringe 421 and communicated with each other, and after the extraction head 41 extracts the suction head 46, the suction head 46 is communicated with the injection assembly through the extraction head 41. The injection drive 423 may be a motor, preferably a stepper motor. The industrial control module 5 is electrically connected with the injection driving device, when a sample or a reagent needs to be sucked, the industrial control module 5 drives the injection driving device to move, so as to drive the piston 422 to move upwards, the pressure in the suction head 46 is reduced, the sample or the reagent can be sucked by the suction head 46, when the sample or the reagent needs to be injected, the industrial control module 5 drives the injection driving device 423 to drive the piston 422 to move downwards, the pressure in the suction head 46 is increased, and the sample or the reagent is discharged from the suction head 46.
The multi-channel sample adding device 4 is slidably connected with the upper part of the casing 1 in the following way: the upper end part of the extracting slide block in the multi-channel sample adding device 4 is connected with a slide seat 47, a slide rail 16 is arranged on a top plate 13 of the machine shell 1, a driving wheel 17 driven by a motor is arranged at one end part of the slide rail 16, a driven wheel 18 and a synchronous belt 19 tensioned between the driving wheel 17 and the driven wheel 18 are arranged at the other end part of the slide rail 16, the synchronous belt 19 is matched with the slide seat 47, and the industrial control module 5 is electrically connected with the motor. When reagent and sample need to be transferred, the driving motor of the industrial control module 5 drives the synchronous belt 19 to rotate, the sliding seat 47 is driven to slide back and forth through the rotation of the synchronous belt 19, and then the multichannel sample adding device 4 is driven to move back and forth to transfer the reagent or the sample, so that the sample adding work is completed.
The industrial control module 5 comprises a touch screen industrial control computer 51 and a mounting cross beam 52, wherein the touch screen industrial control computer 51 is mounted on the rack 11 above the cabin door 2 through the mounting cross beam 52. The detection personnel operate the touch screen industrial personal computer 51 to control and operate the nucleic acid amplification detection analyzer, so that the full-automatic operation of nucleic acid extraction, amplification and detection is realized.
The working principle of the full-automatic nucleic acid amplification detection analyzer is as follows:
preparing to operate a touch screen industrial personal computer, setting corresponding parameters according to detection requirements, starting a full-automatic nucleic acid amplification detection analyzer, sending out a sliding frame of a nucleic acid extraction and amplification device, placing a test tube filled with a sample on a sample carrier, placing a suction head on the extraction carrier, and withdrawing the sliding frame;
(II) the motor of the extraction suction head rotates to drive the driving wheel to rotate, the driving synchronous belt rotates to drive the sliding seat to move, the multi-channel sampling device moves forwards to enable the extraction head to be aligned with the suction head on the extraction carrier (as shown in figure 21a), the desorption head driving mechanism drives the desorption head plate to move upwards to the uppermost end of the extraction head, the extraction driving mechanism drives the extraction head, the injection assembly and the desorption head plate to move downwards together, the extraction head is inserted into a preset position in the suction head opening, the extraction head and the suction head are connected together in an interference fit manner (as shown in figure 21b), and the extraction driving mechanism drives the extraction head and the suction head to move upwards together to separate from the extraction carrier (as;
(III) the injection driving device of the sample sucking and injecting assembly pushes the piston downwards to the lowest part of the injection tube, the multi-channel sample adding device moves to drive the suction head to be aligned with the opening of the sample test tube (as shown in figure 22a), the extraction driving mechanism drives the suction head and the desorption head plate to move downwards together, so that the suction head is inserted into the sample test tube (as shown in figure 22b), the injection driving device drives the piston to move upwards, the suction head sucks a certain amount of sample (as shown in figure 22c), and the extraction driving mechanism drives the suction head and the desorption head plate to move upwards (as shown in figure 23 a);
(IV) the sample-adding multi-channel sample-adding device moves to drive the suction head to move, so that the suction head is aligned with a consumable port on the extraction carrier (as shown in figure 23b), the extraction driving mechanism drives the suction head and the desorption head plate to move downwards, so that the suction head is inserted into the consumable, the injection driving device drives the piston to move downwards, a certain amount of sample is added into the consumable (23c), and the extraction driving mechanism drives the suction head and the desorption head plate to move upwards to finish one sample-adding work;
heating a heating membrane of the incubation module, and performing heat incubation only after extraction in consumables through conduction of a heating block; certainly, when incubating, can also make injection drive arrangement drive piston reciprocate, let the mixed liquid pass in and out the suction head many times, reach the effect of mixing mixed liquid, realize hatching simultaneously mixing.
Sixthly, moving or rotating the magnetic absorption device to approach the consumable material, and absorbing magnetism on the side surface or the bottom surface of the consumable material;
(VII) after the liquid absorption and sample addition of all the holes of the desorption head are finished, the multi-channel sample adding device moves to drive the suction head to move, so that the suction head is aligned with the suction head hole on the extraction carrier (as shown in figure 24a), the suction head and the desorption head plate move downwards and are inserted into the suction head hole (as shown in figure 24b), the desorption head driving mechanism pushes the desorption head plate to move downwards, so that the suction head is separated from the extraction head (as shown in figure 24c), the suction head falls into the suction head hole of the extraction carrier by means of gravity, and the desorption head driving mechanism drives the desorption head plate to move upwards, so that the work of the desorption head is finished;
(eighth) pipetting according to the suction head connected with the suction head, moving the multi-channel sample adding device to drive the suction head to move, aligning the suction head with the consumable on the extraction carrier (as shown in figure 22a), moving the suction head and the desorption head plate downwards by the extraction driving mechanism to be inserted into the consumable (as shown in figure 22b), moving the piston upwards by the injection driving mechanism, sucking a certain amount of liquid by the suction head (as shown in figure 22c), and moving the suction head and the desorption head plate upwards by the extraction driving mechanism (as shown in figure 23 a); the multi-channel sample adding device moves to drive the suction head and the desorption head plate to move, so that the suction head is aligned with a detection consumable hole on a detection consumable carrier (as shown in figure 23b), the suction head and the desorption head plate move downwards to be inserted into the detection consumable hole by the extraction driving mechanism, the injection driving device drives the piston to move downwards, a certain amount of liquid is added into the detection consumable (as shown in figure 23c), and the suction head and the desorption head plate are driven to move upwards by the extraction driving mechanism;
(ninth), the fluorescence detection oscillation driving mechanism drives the detection consumable carrier to oscillate on the oscillation mechanism along the horizontal direction, front, back, left and right, and drives the reagent in the detection consumable to rotate along the inner wall of the detection consumable pipe, so that uniform vortex mixing is realized; meanwhile, after the LED light source emits light, the light is condensed by the excitation light source condensing lens group, then excitation light with a specified wavelength is obtained by the filter plate, the excitation light penetrates through the excitation light source hole and irradiates on a reagent in the consumable, the reagent emits fluorescence with another wavelength to the periphery, part of the fluorescence with the other wavelength is emitted into the fluorescence detection hole, the fluorescence with the specified wavelength is obtained through the fluorescence condensing lens group and the second filter, the fluorescence with the specified wavelength is collected on the photodiode, and the intensity of analysis light can complete conventional nucleic acid amplification detection.
Preferably, the multi-channel sample adding device can also be realized as a capping gland for detecting consumables, wherein the tube cap 8 is placed on the extraction carrier. (1) The extraction tube cap multi-channel sample adding device 4 moves forwards to enable the extraction head to be aligned with the tube cap on the extraction carrier (as shown in figure 25a), the desorption head driving mechanism drives the desorption head plate to move upwards to the uppermost end of the extraction head, the extraction driving mechanism drives the extraction head, the injection assembly and the desorption head plate to move downwards together, the extraction head is inserted into the tube cap (as shown in figure 25b), the extraction head and the tube cap are connected together in an interference fit manner, and the extraction driving mechanism drives the extraction head and the tube cap to move upwards together to separate from the extraction carrier (as shown in figure 25 c); (2) adding lid multichannel application of sample device and removing and drive the tube cap and remove, let the tube cap align with the pipe on the detection consumptive material carrier (as fig. 26a), it will the tube cap to draw actuating mechanism, it down moves the insertion intraductal to take off suction head board (as fig. 26b), it promotes to take off suction head board and moves down to take off suction head board, it is intraductal to impress the tube cap (as fig. 26c), it drives and draws the head and up move to draw actuating mechanism, it continues to drive and takes off suction head board and push down to take off suction head board actuating mechanism, it separates with the tube cap to take off suction head board actuating mechanism and drive, it upwards moves to take off suction head board to take off suction head.
Further, the air filtering module 6 is also included, and the air filtering module 6 includes a lower cover 61 mounted on the top plate, an upper cover 62 buckled on the lower cover, filter cotton 63 disposed in the lower cover 61, an air outlet 64 disposed on the upper cover 62, and an exhaust fan 65 mounted at the lower end of the air outlet. The air containing the aerosol in the detector enters the air filtering module from the groove below the lower cover under the action of the exhaust fan, pollutants such as the aerosol are adsorbed on the filtering surface through the filtering of the filter cotton, and the filtered air meeting the emission standard is discharged from the air outlet, so that the pollution to indoor or outdoor environment can be effectively reduced.
Furthermore, a sample scanning module 7 is also provided, the sample scanning module 7 comprises a scanner 71, the scanner is used for identifying a bar code on a sample or a reagent, and the scanner 71 is fixed on a cross beam through a bracket and is arranged on the rack 11 between the cabin door and the industrial control module through the cross beam. Preferably, an ultraviolet sterilizing lamp 72 is provided on the side of the beam facing the inside of the housing to sterilize the inside of the detector with ultraviolet light. Preferably, a status indicator lamp 73 is further provided, and the status indicator lamp is mounted below the side of the cross beam and can display the status of the detector in real time.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A full-automatic nucleic acid amplification detection analyzer is characterized by comprising a machine shell with an opening on one side, a cabin door arranged at the opening of the machine shell, a nucleic acid extraction and amplification device horizontally and vertically arranged at the bottom of the machine shell towards the cabin door, a multi-channel sample adding device arranged above the nucleic acid extraction and amplification device and connected to the upper end of the machine shell in a sliding manner, and an industrial control module;
the nucleic acid extraction and amplification device comprises a sliding frame which can be sent out or retracted along the hatch door and a sending-out driving device; a sample carrier, an incubation module, an extraction carrier for loading extraction consumables, a magnetic attraction device arranged below the extraction carrier and a fluorescence detection device with oscillation are sequentially arranged on the sliding frame from the cabin door inwards;
the multichannel sample adding device comprises an extraction head for extracting a suction head, an injection assembly, an extraction driving mechanism, a suction head removing plate and a suction head removing driving mechanism, wherein the injection assembly is connected with the extraction head and is communicated with the extraction head, the extraction driving mechanism is used for driving the extraction head to move up and down, and the suction head removing driving mechanism is used for driving the suction head removing plate to move up and down.
2. The automatic nucleic acid amplification detection analyzer of claim 1, wherein the housing comprises a frame, a top plate and a bottom plate respectively fixed to the upper and lower ends of the frame, and side plates surrounding three sides of the frame; the lower end part of the cabin door is rotatably pivoted on the bottom plate at the opening of the shell, and a torsional spring is sleeved at the pivoting part of the cabin door and the bottom plate.
3. The automatic nucleic acid amplification detection analyzer of claim 2, wherein the sliding rack is provided with a wheel for pushing the hatch to turn outwards and open when the sliding rack is delivered.
4. The automatic nucleic acid amplification detection analyzer of claim 2, wherein a slide rail is disposed on a top plate of the housing, a driving wheel driven by a motor is disposed at one end of the slide rail, a driven wheel and a synchronous belt tensioned between the driving wheel and the driven wheel are disposed at the other end of the slide rail, and a slide seat is disposed at the top of the multi-channel sample adding device and driven by the synchronous belt to slide back and forth on the slide rail.
5. The automatic nucleic acid amplification detection analyzer of claim 2, wherein the nucleic acid extraction and amplification device further comprises a support frame, the bottom plate is provided with a stopper, the support frame is mounted on the bottom plate through the stopper, the carriage is disposed on the support frame, and the support frame cooperates with the feeding driving device to feed or retract the carriage to the chamber door.
6. The automatic nucleic acid amplification detection analyzer of claim 5, wherein the feeding driving device comprises a feeding motor, a feeding driving wheel, a feeding synchronous belt and two feeding driven wheels, the feeding driving wheel is disposed on an output shaft of the feeding motor, the two feeding driven wheels are disposed below the driving wheel, two ends of the feeding synchronous belt are fixed to the supporting frame, and the two feeding driven wheels press the feeding synchronous belt against the feeding driving wheel.
7. The automatic nucleic acid amplification detecting analyzer according to claim 2, wherein the fluorescence detecting device with oscillation comprises a fluorescence detecting module, an oscillating mechanism disposed above the fluorescence detecting module, a detecting consumable carrier disposed on the oscillating mechanism, and an oscillation driving mechanism for driving the detecting consumable carrier to move back and forth and left and right on the oscillating mechanism along a horizontal direction.
8. The automatic nucleic acid amplification detection analyzer of claim 2, further comprising an air filtration module, wherein the air filtration module comprises a lower cover and an upper cover, filter cotton disposed in the lower cover, an air outlet disposed on the upper cover, and an exhaust fan mounted at the lower end of the air outlet; the lower cover is installed above the top plate.
9. The automatic nucleic acid amplification detection analyzer of claim 2, wherein the industrial control module comprises a touch screen industrial personal computer and a mounting beam, the touch screen industrial personal computer is mounted on the beam, and the beam is mounted on the rack above the hatch.
10. The automated nucleic acid amplification detection analyzer of claim 9, further comprising a sample scanning module mounted on the rack between the hatch and the industrial control module.
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