CN215887043U - Nucleic acid extraction amplification device - Google Patents

Abstract

The utility model particularly discloses a nucleic acid extraction and amplification device, which comprises a support frame and a sending-out module which is arranged on the support frame and slides relative to the support frame; the supporting frame comprises a bottom plate and supporting plates arranged on two sides of the bottom plate; the delivery module comprises a drawer frame and a driving module, wherein the drawer frame is formed by enclosing a sliding bottom plate and supports arranged on two sides of the sliding bottom plate; the drawer frame is sequentially provided with an incubation module, an extraction carrier for loading consumables, a magnetic absorption module and a fluorescence detection module along the length direction; the driving module is matched with the supporting frame and used for sending out or withdrawing the drawer frame to the supporting frame. The structure is simple and clear, the operation is convenient, and the cross contamination is effectively avoided.

Description

Nucleic acid extraction amplification device

Technical Field

The utility model relates to the field of biological sample detection and analysis, in particular to a nucleic acid extraction and amplification device.

Background

Nucleic acid (including deoxyribonucleic acid DNR and ribonucleic acid RNA) exists in various organisms, a plurality of processes such as cracking, combining, shaking, magnetic attraction, washing, elution, amplification, detection analysis and the like are required for the extraction, detection and analysis of the nucleic acid in a biological sample, a specific device is required for each process, the number of the processes is large, the number of devices corresponding to each process is large, and the conventional nucleic acid detection instrument is inconvenient to operate, particularly to place and replace test consumables. In addition, the disordered stacking of the plurality of devices causes the devices in one process to enter the space in another process, and the disordered operation easily causes cross contamination between samples.

SUMMERY OF THE UTILITY MODEL

In view of the above, there is a need for a nucleic acid extracting and amplifying device that is simple and clear in structure, convenient to operate, and capable of effectively avoiding cross contamination.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a nucleic acid extraction amplification device comprising: the device comprises a support frame and a sending-out module which is arranged on the support frame and slides relative to the support frame; the supporting frame comprises a bottom plate and supporting plates arranged on two sides of the bottom plate; the delivery module comprises a drawer frame and a driving module, wherein the drawer frame is formed by enclosing a sliding bottom plate and supports arranged on two sides of the sliding bottom plate; the drawer frame is sequentially provided with an incubation module, an extraction carrier for loading consumables, a magnetic absorption module and a fluorescence detection module along the length direction; the driving module is matched with the supporting frame and used for sending out or withdrawing the drawer frame to the supporting frame.

Further, the drawer frame is provided with a linear guide rail along the length direction, the support frame is provided with a sliding block, and the linear guide rail is in sliding fit with the sliding block.

Furthermore, the linear guide rail is arranged on the outer side surface of the support, and the sliding block is arranged on the inner side surface of the support plate.

Furthermore, the support frame is also provided with a pressing plate, one end of the pressing plate is rotatably connected with the support plate, and the other end of the pressing plate is rotatably opened or closed relative to the support plate.

Furthermore, a rebound part is arranged between the pressing plate and the supporting plate, the rebound direction of the rebound part is the direction for opening the pressing plate, the pressing plate is provided with a first connecting rod, the supporting plate is provided with a second connecting rod, the first end of the first connecting rod is hinged with the pressing plate, the first end of the second connecting rod is hinged with the supporting plate, and the second end of the first connecting rod is hinged with the second end of the second connecting rod; the outer side surface of the support is provided with a collision wheel used for limiting the first connecting rod or the second connecting rod; when the drawer frame is retracted into the support frame, the first connecting rod or the second connecting rod is pressed by the impact wheel, the first connecting rod and the second connecting rod are bent relatively, and the pressing plate is closed relative to the support plate; when the drawer frame is sent out of the support frame, the first connecting rod or the second connecting rod is loosened by the collision wheel, and the other end of the pressing plate is opened relative to the top of the supporting plate under the effect of resilience force of the resilience piece.

Furthermore, the magnetic attracting module is a translation magnetic attracting device, the translation magnetic attracting device comprises a guide rail assembly, a magnetic attracting assembly and a driving assembly, the guide rail assembly comprises a rail and a sliding block in sliding fit with the rail, the magnetic attracting assembly comprises a magnet, and the driving assembly is in butt joint with the magnetic attracting assembly or the driving assembly is in butt joint with the sliding block; the magnetic attraction component is arranged on the sliding block, and the driving component is used for driving the magnetic attraction component to slide on the track.

Further, inhale the magnetism module and inhale the magnetism device for the rotation type, the rotation type is inhaled the magnetism device and is included: the magnetic iron comprises a rotatable rotating shaft, a magnet frame arranged on the rotating shaft, and a first magnet and a second magnet which are arranged at two end parts of the magnet frame, wherein the first magnet and the second magnet respectively extend out of two sides of the rotating shaft; when the rotating shaft rotates to a first position, the first magnet is positioned at a first magnetic suction station; when the rotating shaft rotates to the second position, the second magnet is located at the second magnetic suction station.

Furthermore, a handle is arranged at the front end of the drawer frame.

Furthermore, the drawer frame is provided with an upper cover which covers the driving module.

Further, drive module including set up in the motor of drawer frame, first from driving wheel and second follow the driving wheel, the output shaft of motor is equipped with the action wheel, drive module still includes the hold-in range, the both ends of hold-in range are fixed in the support frame, the motor is located one side of hold-in range first from the driving wheel with the second is located from the driving wheel the opposite side of hold-in range, first from the driving wheel with the second will from the driving wheel the hold-in range compress tightly in the action wheel.

The utility model has the beneficial effects that:

the parts of the nucleic acid extraction and amplification device are arranged in a modularized manner, the modularized fluorescence detection module, the magnetic absorption module, the incubation module and the driving module are arranged on the drawer frame, the whole drawer frame (the whole drawer frame together with the fluorescence detection module, the magnetic absorption module, the incubation module and the driving module) can be sent out from the supporting frame through the sliding connection of the drawer frame and the supporting frame, and the whole drawer frame can be retracted into the supporting frame. All operation actions in the detection process are concentrated on the position of the drawer frame, the operation is simple, and if the nucleic acid detector adopts the nucleic acid extraction and amplification device, the drawer frame can also extend out of the shell of the whole nucleic acid detector, so that consumable parts can be conveniently placed and replaced, and the operation is convenient. Simultaneously, be equipped with in proper order along length direction on the drawer frame and hatch the module, load the extraction carrier of consumptive material, arrange in and draw the magnetism module of carrier below, and fluorescence detection module, hatch the module, the extraction carrier of loading the consumptive material, arrange in and draw the magnetism module of carrier below, and fluorescence detection module all sets up a plurality of detection channels side by side, the drawer frame stretch out/retrieve the direction and hatch, magnetism is inhaled, fluorescence detection's array orientation is unanimous, to the sample of same passageway, reagent, the suction head of drawing sample or reagent, their motion trail only covers and hatches in this passageway, magnetism is inhaled, fluorescence detection's position, and can not span to other passageways on, cross contamination between the different passageways has been avoided.

Drawings

FIG. 1 is a schematic diagram of a first embodiment of a nucleic acid extraction and amplification apparatus;

FIG. 2 is an exploded view of a nucleic acid amplification apparatus according to an embodiment;

FIG. 3 is a schematic view of a partial structure of a nucleic acid extraction amplification apparatus according to an embodiment;

FIG. 4 is a schematic diagram showing a half-sending state of the nucleic acid isolation and amplification apparatus according to the embodiment;

FIG. 5 is a partially disassembled schematic view of FIG. 4;

FIG. 6 is an enlarged view of a portion of FIG. 5;

FIG. 7 is a schematic diagram illustrating a driving principle of a driving module and a supporting frame according to an embodiment;

FIG. 8 is a schematic diagram showing a configuration of a nucleic acid extracting/amplifying apparatus according to an embodiment in a feeding state;

FIG. 9 is a schematic view illustrating the opening and closing of a pressing plate according to an embodiment;

FIG. 10 is a schematic diagram illustrating the opening and closing of a pressing plate according to the first embodiment;

FIG. 11 is a schematic diagram of a sample carrier of an embodiment in preparation for loading sample tubes;

FIG. 12 is a schematic diagram of a consumable part of an extraction carrier preparation apparatus according to an embodiment;

FIG. 13 is a schematic view of a magnet of an exemplary embodiment of a magnet attracting module at a rear end;

FIG. 14 is a schematic view of a magnet attracting module according to an embodiment of the present invention;

FIG. 15 is a schematic structural view of a second rotary magnetic attraction module according to an embodiment;

FIG. 16 is a schematic view of a first magnet positioned at a first magnetic attraction station according to an embodiment;

fig. 17 is a schematic structural view illustrating a second magnet positioned at a second magnetic attraction station according to an embodiment.

Reference numerals:

1. the device comprises a support frame, 11, a bottom plate, 12, a support plate, 14, a pressure plate, 141, a first connecting rod, 142, a second connecting rod, 143, a resilient member, 2, a drawer frame, 21, a driving module, 211, a control circuit, 212, a motor, 213, a driving wheel, 214, a synchronous belt, 215, a first driven wheel, 216, a second driven wheel, 22, an upper cover, 23, a fluorescence detection module, 24, a magnetism absorption module, 25, an incubation module, 26, a test tube rack, 27, an extraction carrier, 28, a support, 281, a linear guide rail, 282, a collision wheel, 284, a handle, 29, a sliding bottom plate, 3, a sample test tube, 4, a consumable, 51, a guide rail assembly, 511, a rail, 512, a sliding block, 52, a magnetic absorption assembly, 521, a magnet, 53, a translation type driving assembly, 61, a rotating shaft, 62, a magnet rack, 63, a first magnet, 64, a second magnet, 65 and a rotation type driving assembly.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be further clearly and completely described below with reference to the embodiments of the present invention. It should be noted that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", and the like, are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus are not to be construed as limiting the present invention.

The terms "first," "second," "third," "fourth," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, the definitions of "first", "second", "third", "fourth" features may explicitly or implicitly include one or more of such features.

The first embodiment is as follows:

as shown in FIGS. 1 to 14, the nucleic acid isolation and amplification apparatus of the present invention is shown. As shown in fig. 1-2, comprising: the device comprises a drawer frame 2 and a support frame 1, wherein the drawer frame 2 is provided with a fluorescence detection module 23, a magnetism absorption module 24, an incubation module 25 and a driving module 21; wherein, drive module 21 and support frame 1 dock, and drawer frame 2 and support frame 1 sliding fit, drive module 21 are used for driving drawer frame 2 to send out support frame 1, or drive drawer frame 2 and withdraw in support frame 1. The drawer frame 2 is slidably mounted in the support frame 1 to form a drawer-like structure.

As shown in fig. 1, 2, 11 and 12, the drawer frame 2 is also provided with an extraction carriage 27 for loading the consumables 4 and with a sample carriage for loading sample tubes. The drawer frame 2 is sequentially provided with the sample carrier, the incubation module 25, the magnetism absorption module 24, the fluorescence detection module 23 and the driving module 21 from the front end to the tail end, and the extraction carrier 27 is arranged above the incubation module 25.

As shown in fig. 3, the drawer frame 2 includes a sliding bottom plate 29 and two brackets 28 respectively disposed on two sides of the sliding bottom plate 29, the fluorescence detection module 23, the magnetic absorption module 24, the incubation module 25, and the driving module 21 are disposed between the two brackets 28, the support frame 1 includes a bottom plate 11 and two support plates 12 respectively disposed on two sides of the bottom plate 11, and the drawer frame 2 is disposed between the two support plates 12. Drawer frame 2 constitutes the structure of a similar drawer tray, with fluorescence detection module 23, magnetism module 24, hatch module 25, and drive module 21 install in this drawer tray of drawer frame 2, install drawer frame 2 integral erection in support frame 1 again, adopt the mode of getting of similar drawer, conveniently load consumptive material 4 and sample test tube when drawer frame 2 sees off, then detect reagent when drawer frame 2 withdraws, then drawer frame 2 sees off once more, take off and draw consumptive material 4.

As shown in fig. 1-5, the supporting frame 1 is provided with a sliding block, the drawer frame 2 is provided with a linear guide 281, and the sliding block is in sliding fit with the linear guide 281. Relative motion between the drawer frame 2 and the support frame 1 is realized through the mode of sliding fit of the sliding block and the guide rail, the sliding of the drawer frame 2 is stable, the moving direction is controllable, the position of the drawer frame 2 which is sent out and retracted is controllable, and the detection of reagents is facilitated. The linear guide 281 is arranged on the outer side of the support 28 and the slide is arranged on the inner side of the support plate 12. The outer side surfaces of the two supports 28 of the drawer frame 2 are provided with linear guide rails 281, and correspondingly, the inner side surfaces of the two support plates 12 are provided with slide blocks, so that the movement of the drawer frame 2 is more stable, and the deviation of the movement direction is avoided.

As shown in fig. 1 to 7, the driving module 21 includes a motor 212, a first driven wheel 215, and a second driven wheel 216 disposed on the drawer frame 2, an output shaft of the motor 212 is provided with a driving wheel 213, the driving module 21 further includes a timing belt 214, two ends of the timing belt 214 are fixed on the support frame 1, the motor 212 is disposed on one side of the timing belt 214, the first driven wheel 215 and the second driven wheel 216 are disposed on the other side of the timing belt 214, and the first driven wheel 215 and the second driven wheel 216 press the timing belt 214 against the driving wheel 213. The two ends of the synchronous belt 214 are fixed to the support frame 1, and when the driving wheel 213 rotates, the first driven wheel 215 and the second driven wheel 215 press the synchronous belt 214 against the driving wheel 213, and the friction force between the synchronous belt 214 and the driving wheel 213 causes the driving wheel 213 to roll relative to the synchronous belt 214, thereby driving the drawer frame 2 to slide relative to the support frame 1. The synchronous belt 214 is adopted to convert the rotation of the driving wheel 213 into translation to drive the drawer frame 1 to slide, so that the drawer frame 2 can be stably moved, the position of the motor 212 can be flexibly designed, the motor 212 is compactly arranged at the tail end of the drawer frame 2, and the whole device occupies a smaller space. In this embodiment, as shown in fig. 7, the first driven wheel 215 and the second driven wheel 216 are respectively located at two sides of the driving wheel 213, but not limited to this embodiment, the first driven wheel 215 and the second driven wheel 216 may be disposed at the same side of the driving wheel 213, as long as the timing belt 214 can be pressed on the driving wheel 213, so that the driving wheel 213 can drive the drawer frame 2 to move when rotating. When sending out drawer frame 2 is whole from support frame 1, it is convenient to load consumptive material 4 and sample test tube 3, when drawer frame 2 is whole to be withdrawed in support frame 1, drawer frame 2 is withdrawed in support frame 1 together with consumptive material 4 and sample test tube 3, the detection operation to the sample in the sample test tube 3 is concentrated in the position at drawer frame 2, each operation action in the testing process is all concentrated in the position of drawer frame 2, the position of operation action is concentrated, avoid each group of nucleic acid to draw and amplify cross contamination between the device. In addition, the whole drawer frame 2 (the whole drawer frame 2 together with the fluorescence detection module 23, the magnetism absorption module 24, the incubation module 25 and the drive module 21) and the whole support frame 1 are a group of complete nucleic acid extraction and amplification devices, and a plurality of groups of such nucleic acid extraction and amplification devices can be adopted in parallel according to needs, and the nucleic acid extraction and amplification devices are not interfered with each other and are not polluted by each other; the maintenance and installation of each group of test modules are independent, so that the production and the maintenance are convenient.

As shown in fig. 1 to 4, the drawer frame 2 is further provided with an upper cover 22, and the upper cover 22 covers the driving module 21. The upper cover 22 can protect the driving module 21 from colliding with the driving module 21 on the one hand and from liquid dripping to corrode the driving module 21 on the other hand. In addition, the drive module 21 is separated from the fluorescence detection module 23, the magnetism absorption module 24 and the incubation module 25, so that the fluorescence detection module 23, the magnetism absorption module 24 and the incubation module 25 can be replaced to continuously reserve the drive module 21, and the maintenance cost is reduced. Fluorescence detection module 23, magnetic attraction module 24, and incubation module 25 are relatively easily worn components relative to the durability of drive module 21, while drive module 21 is relatively durable, and providing drive module 21 with upper cover 22 may better extend the useful life of drive module 21.

As shown in fig. 1-8, the supporting frame 1 is further provided with a pressing plate 14, and one end of the pressing plate 14 is rotatably engaged with the supporting plate 12. After drawer frame 2 loads consumptive material 4, withdraw drawer frame 2 in support frame 1, clamp plate 14 is the closed condition this moment, and clamp plate 14 compresses tightly consumptive material 4 on drawing carrier 27, does benefit to the heating and incubate and can prevent that consumptive material 4 from shifting.

As shown in fig. 9 and 10, a resilient member 143 is disposed between the pressing plate 14 and the supporting plate 12, a resilient direction of the resilient member 143 is a direction to open the pressing plate 14, the pressing plate 14 is provided with a first connecting rod 141, the supporting plate 12 is provided with a second connecting rod 142, a first end of the first connecting rod 141 is hinged to the pressing plate 14, a first end of the second connecting rod 142 is hinged to the supporting plate 12, and a second end of the first connecting rod 141 is hinged to a second end of the second connecting rod 142. A bump wheel 282 for limiting the first link 141 or the second link 142 is arranged on the outer side surface of the bracket 28; when the drawer frame 2 is retracted into the supporting frame 1, the striking wheel 282 presses the first connecting rod 141 or the second connecting rod 142, the first connecting rod 141 and the second connecting rod 142 are bent relatively, the distance between the first end of the first connecting rod 141 and the first end of the second connecting rod 142 is reduced, the pressing plate 14 is pulled down, and the pressing plate 14 is closed relative to the supporting plate 12, as shown in fig. 10; when the drawer frame 2 is taken out of the support frame 1, the striking wheel 282 releases the first link 141 or the second link 142, the first link 141 and the second link 142 are relatively spread, the distance between the first end of the first link 141 and the first end of the second link 142 is increased, and the pressing plate 14 is opened relative to the top of the support plate 12 by the resilient force of the resilient member 143, as shown in fig. 9. This allows the pressure plate 14 to be automatically opened and closed in accordance with the movement of the drawer frame 2. The "springing" of the resilient member 143 means that the pressing plate 14 can be sprung open, and the resilient member 143 is a coil spring in this embodiment, but is not limited to this embodiment, and any material (including elastic material and non-elastic material) or any part or assembly that can achieve this function can be used for the resilient member 143.

As shown in fig. 5, the front ends of the two brackets 28 are provided with handles 284, and the handles 284 are used for manually pulling out or pushing in the drawer frame 2 from the support frame 1.

In the specific operation:

the motor 212 rotates to drive the drawer frame 2 to be sent out of the support frame 1, the collision wheel 282 releases the first connecting rod 141 or the second connecting rod 142, the pressing plate 14 is opened along with the movement of the drawer frame 2, the consumable 4 is released, and the drawer frame 2 is sent out of the support frame 1, as shown in fig. 8 and 14;

(II) unloading the original consumable items 4 if the consumable items 4 are loaded on the pick-up carriage 27; if the test tube rack 26 is loaded with the sample test tubes 3, the original sample test tubes 3 are unloaded, and if no consumable 4 is available, no sample test tube 3 is available, the step is omitted;

(III) loading the consumable 4 to the extraction carrier 27, and loading the sample test tube containing the sample to the test tube rack 26;

(IV) the motor 212 rotates reversely to drive the drawer frame 2 to retract into the box body 5, the pressing plate 14 is closed when the collision wheel 282 presses the first connecting rod 141 or the second connecting rod 142, and the consumable 4 is pressed by the pressing plate 14, as shown in FIG. 1;

fifthly, carrying out detection such as incubation, magnetism absorption, oscillation, detection and the like; in this embodiment, as shown in fig. 13 and 14, the magnetic attraction module 24 is a translational magnetic attraction structure, the magnetic attraction module 24 includes a guide rail assembly 51, a magnetic attraction assembly 52 and a translational driving assembly 53, the guide rail assembly 51 includes a rail 511 and a slider 512 slidably engaged with the rail 511, the magnetic attraction assembly 52 includes a magnet 521, and the translational driving assembly 53 is in butt joint with the magnetic attraction assembly 52 or the translational driving assembly 53 is in butt joint with the slider 512; wherein, the magnetic attraction component 52 is installed on the sliding block 512, and the translational driving component 53 is used for driving the magnetic attraction component 52 to slide on the track. Realize the translation of magnetism subassembly 52 of inhaling through rail set 51, when needing to inhale magnetism, inhale magnetism subassembly 52 and remove to the front end, as shown in FIG. 14, let magnet 521 be close to the test tube and inhale magnetism, when stopping inhaling magnetism, inhale magnetism subassembly 52 and return to the rear end with magnetism, as shown in FIG. 13, let magnet 521 keep away from the test tube. The guide rail component 51 adopts the sliding fit of the sliding block 512 and the track 511, the whole device is stable in movement, and the magnetic absorption position is favorably controlled, so that the adsorption state of magnetic beads is accurately controlled, and the extraction purity is improved. And the structure is small and the occupied space is small.

And (VI) after the detection is finished, repeating the step one.

As shown in fig. 1, in this embodiment, the incubation module 25, the extraction carrier 27 for loading consumables, the magnetic absorption module 24 disposed below the extraction carrier 27, and the fluorescence detection module 23 are disposed in parallel along the width direction of the drawer frame 2, and the drawer frame 2 is sequentially disposed with the incubation module 25, the extraction carrier 27 for loading consumables, the magnetic absorption module 24 disposed below the extraction carrier, and the fluorescence detection module 23 along the length direction, where the extending/retrieving direction of the drawer frame 2 is also the length direction of the drawer frame 2, the extending/retrieving direction of the drawer frame 2 is the same as the direction in which the sample undergoes incubation, magnetic absorption, and fluorescence detection, and for the sample, reagent, the suction head for extracting the sample or reagent in the same channel, their motion trajectories only cover the positions of incubation, magnetic absorption, and fluorescence detection in the channel, and do not cross over other channels, cross-contamination between different channels is avoided.

Example two

The difference between the second embodiment and the first embodiment is that:

as shown in fig. 15-17, the magnetic attraction module 24 is a rotary magnetic attraction structure, and the magnetic attraction module 24 includes: the magnetic driving device comprises a rotatable rotating shaft 61, a rotary driving component 65 connected with one end of the rotating shaft 61, a magnet frame 62 arranged on the rotating shaft 61, and a first magnet 63 and a second magnet 64 arranged at two ends of the magnet frame 62, wherein the first magnet 63 and the second magnet 64 respectively extend out of two sides of the rotating shaft 61; the rotary driving assembly 65 is used for driving the rotating shaft 61 to rotate: when the rotating shaft 61 rotates to the first position, the first magnet 63 is located at the first magnetic attraction station, as shown in fig. 16; when the rotating shaft 61 rotates to the second position, the second magnet 64 is located at the second magnetic attraction station, as shown in fig. 17. First magnet 63 and second magnet 64 set up in the both ends of magnet frame 62, magnet frame 62 middle part is equipped with pivot 61, magnet frame 62 and pivot 61 form the structure of similar seesaw, realize first magnet 63 or second magnet 64 "being close to and draw the consumptive material" or "keeping away from and drawing the consumptive material" through rotating pivot 61, not only can realize drawing the heating incubation function of consumptive material, and can freely switch over the different magnetism position of inhaling, effectual control draws the magnetic bead in the reagent and concentrates, satisfy the reagent volume transfer of not equidimension volume.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. A nucleic acid extraction and amplification device, comprising:

the device comprises a support frame and a sending-out module which is arranged on the support frame and slides relative to the support frame;

the supporting frame comprises a bottom plate and supporting plates arranged on two sides of the bottom plate;

the delivery module comprises a drawer frame and a driving module, wherein the drawer frame is formed by enclosing a sliding bottom plate and supports arranged on two sides of the sliding bottom plate; the drawer frame is sequentially provided with an incubation module, an extraction carrier for loading consumables, a magnetic absorption module and a fluorescence detection module along the length direction;

the driving module is matched with the supporting frame and used for sending out or withdrawing the drawer frame to the supporting frame.

2. The nucleic acid extraction and amplification device according to claim 1, wherein the drawer frame is provided with a linear guide rail along a length direction, the support frame is provided with a slide block, and the linear guide rail is in sliding fit with the slide block.

3. The nucleic acid isolation and amplification device of claim 2, wherein the linear guide is disposed on an outer side surface of the holder, and the slider is disposed on an inner side surface of the support plate.

4. The nucleic acid extraction and amplification device of claim 1, wherein the support frame is further provided with a pressing plate, one end of the pressing plate is rotatably connected to the support plate, and the other end of the pressing plate is rotatably opened or closed relative to the support plate.

5. The nucleic acid extraction and amplification device according to claim 4, wherein a resilient member is provided between the pressing plate and the support plate, the resilient member has a resilient direction in which the pressing plate is opened, the pressing plate is provided with a first link, the support plate is provided with a second link, a first end of the first link is hinged to the pressing plate, a first end of the second link is hinged to the support plate, and a second end of the first link is hinged to a second end of the second link;

the outer side surface of the support is provided with a collision wheel used for limiting the first connecting rod or the second connecting rod;

when the drawer frame is retracted into the support frame, the first connecting rod or the second connecting rod is pressed by the impact wheel, the first connecting rod and the second connecting rod are bent relatively, and the pressing plate is closed relative to the support plate; when the drawer frame is sent out of the support frame, the first connecting rod or the second connecting rod is loosened by the collision wheel, and the other end of the pressing plate is opened relative to the top of the supporting plate under the effect of resilience force of the resilience piece.

6. The nucleic acid extraction and amplification device according to claim 1, wherein the magnetic attraction module is a translational magnetic attraction device, the translational magnetic attraction device comprises a guide rail assembly, a magnetic attraction assembly and a driving assembly, the guide rail assembly comprises a rail and a slider in sliding fit with the rail, the magnetic attraction assembly comprises a magnet, and the driving assembly is in butt joint with the magnetic attraction assembly or the driving assembly is in butt joint with the slider;

the magnetic attraction component is arranged on the sliding block, and the driving component is used for driving the magnetic attraction component to slide on the track.

7. The nucleic acid extraction and amplification device of claim 1, wherein the magnetic attraction module is a rotary magnetic attraction device, and the rotary magnetic attraction device comprises: the magnetic iron comprises a rotatable rotating shaft, a magnet frame arranged on the rotating shaft, and a first magnet and a second magnet which are arranged at two end parts of the magnet frame, wherein the first magnet and the second magnet respectively extend out of two sides of the rotating shaft;

when the rotating shaft rotates to a first position, the first magnet is positioned at a first magnetic suction station; when the rotating shaft rotates to the second position, the second magnet is located at the second magnetic suction station.

8. The nucleic acid extraction and amplification device according to claim 1, wherein a handle is further provided at the front end of the drawer frame.

9. The nucleic acid extraction and amplification device according to claim 1, wherein the drawer rack is provided with an upper cover which covers the drive module.

10. The nucleic acid extraction and amplification device according to any one of claims 1 to 9, wherein the driving module comprises a motor, a first driven wheel, and a second driven wheel, the motor is disposed on the drawer frame, a driving wheel is disposed on an output shaft of the motor, the driving module further comprises a synchronous belt, two ends of the synchronous belt are fixed to the support frame, the motor is disposed on one side of the synchronous belt, the first driven wheel and the second driven wheel are disposed on the other side of the synchronous belt, and the first driven wheel and the second driven wheel press the synchronous belt against the driving wheel.

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