CN219314942U - Biological sample detection device - Google Patents

Abstract

The application discloses biological sample check out test set relates to biological detection technical field. Biological sample check out test set up including carrying detection device and extracting the transfer device, carry detection device with business turn over storehouse module, PCR detection module and magnetism inhale ultrasonic module and integrate in first frame, extract the transfer device and draw the module with lift rotatory module and sample and integrate in the second frame, first frame and second frame can dismantle the connection. The biological sample detection equipment disclosed by the application can be detached from the conveying detection device and the extracting and transferring device during transportation, and can be used after being assembled with the conveying detection device and the extracting and transferring device during use. And the layout of the internal structure of the biological sample detection equipment is simplified through the modularized integrated layout, so that the structure is more compact, the volume is small, and the transportation, the use and the assembly are more convenient. And the workload of disassembly and assembly is reduced, and the operation efficiency is improved.

Description

Biological sample detection device

Technical Field

The present application relates to the field of biological detection technology, and in particular, to a biological sample detection device.

Background

In vitro diagnosis is an important component of modern detection medicine, and clinical application of in vitro diagnosis extends through the whole process of disease prevention, preliminary diagnosis, treatment scheme selection and curative effect evaluation. Molecular diagnosis (such as nucleic acid detection) is a technical basis for accurate medical treatment and accurate detection, and is also the most rapid sub-industry in-vitro diagnosis. Among them, for example, nucleic acid extraction and detection of a sample using a nucleic acid detecting apparatus is an important task for molecular diagnosis, and thus nucleic acid extraction and detection are required for a nucleic acid detecting apparatus such as a molecular POCT integrated machine.

Nucleic acid detection devices typically employ PCR (Polymerase Chain Reaction ) technology. In the case of nucleic acid detection by PCR, pretreatment such as extraction, transfer, and homogenization of a sample is required. At present, most of nucleic acid detection devices are automated, so that different processing actions are required to be completed through different functional components in pretreatment of detection.

However, the existing nucleic acid detecting equipment integrates different functional components into a whole chassis, the functional components are mixed together and are not reasonably planned and laid out, so that the existing nucleic acid detecting equipment is complex in internal structure, not compact in structure and large in size. When leaving the factory, the whole machine is assembled to be transported, so that a large amount of transportation space is occupied, and a large number of other peripheral functional components are required to be removed in the later period of overhauling and maintaining one functional component, so that a large amount of time is consumed in the process of disassembly, the workload is increased, and the operation efficiency is reduced.

Disclosure of Invention

The purpose of the present application is to provide a biological sample detection device and a biological sample detection method, which are used for solving the defects existing in the prior art.

To achieve the above object, in a first aspect, the present application provides a biological sample detection apparatus for detection of a sample, the biological sample detection apparatus comprising:

The conveying detection device comprises a first rack, a bin inlet and outlet module, a PCR detection module and a magnetic suction ultrasonic module, wherein the bin inlet and outlet module, the PCR detection module and the magnetic suction ultrasonic module are arranged on the first rack, the first rack is also provided with a detection station, the bin inlet and outlet module is used for conveying a kit loaded with samples and reagents to the detection station, the magnetic suction ultrasonic module and the PCR detection module are both arranged corresponding to the detection station, the magnetic suction ultrasonic module is used for conducting magnetic suction and ultrasonic treatment on the samples or the reagents in the kit, and the PCR detection module is used for detecting the samples processed in the kit; and

the sample extracting and transferring device comprises a second rack, a lifting and rotating module and a sample extracting module, wherein the second rack is detachably arranged on the first rack, the lifting and rotating module is arranged on the second rack, the sample extracting module is arranged on the lifting and rotating module and is positioned above the detection station, and the lifting and rotating module is used for driving the sample extracting module to lift and rotate so that the sample extracting module can absorb samples or reagents in the reagent kit for transferring.

As a further improvement of the above technical scheme:

With reference to the first aspect, in one possible implementation manner, two positioning protrusions are arranged at the bottom of the second rack, two ends of each positioning protrusion extend along the length direction of the second rack, and the widths of the two positioning protrusions are matched with the widths between the inner walls of the two side plates of the first rack;

the first rack is a first positioning reference surface along the length direction, one end surface of the first rack, which is close to the detection station, is a second positioning reference surface along the length direction, one end surface of the second rack, which is close to the detection station, and when the first rack and the second rack are assembled, the positioning protrusions are abutted against the inner walls of the side plates, and the first positioning reference surface and the second positioning reference surface are aligned.

With reference to the first aspect, in one possible implementation manner, a connecting piece is disposed between the first rack and the second rack, the connecting piece includes a first connecting portion and a second connecting portion that are integrally connected, planes where the first connecting portion and the second connecting portion are located are perpendicular to each other, where the first connecting portion is detachably connected with a side plate of the first rack, and the second connecting portion is detachably connected with a bottom of the second rack.

With reference to the first aspect, in one possible implementation manner, the in-out bin module is provided with a clamping portion for clamping the kit, and a plurality of liquid storage bins for storing samples or reagents are arranged in the kit, wherein the in-out bin module is used for driving the clamping portion to transfer between the detection station and a feeding station outside the first rack.

With reference to the first aspect, in one possible implementation manner, the in-out bin module includes a lifting frame and a conveying driving mechanism;

the lifting frame is slidably arranged on the first rack, and a clamping part for clamping the reagent box is arranged on the lifting frame;

the conveying driving mechanism is arranged on the first frame and is in transmission connection with the lifting frame, and the conveying driving mechanism is used for driving the lifting frame to drive the clamping part to be close to or far away from the detection station along the length direction of the first frame.

With reference to the first aspect, in one possible implementation manner, the clamping part is provided with a cartridge device, and the cartridge device comprises a cartridge seat, a pressing mechanism, a clamping mechanism and an in-place detection mechanism;

the cartridge seat is arranged on the warehouse-in and warehouse-out module, and is provided with a clamping groove for accommodating the reagent cartridge;

The pressing mechanism can be close to or far away from the clamping groove and is used for clamping or loosening the kit;

the clamping mechanism protrudes from the side wall of the clamping groove into the clamping groove and is used for positioning the kit;

the in-place detection mechanism is used for detecting the kit in the clamping groove and sending an in-place signal of the kit to the in-out bin module.

With reference to the first aspect, in one possible implementation manner, the clamping part is provided with a cartridge device, and the cartridge device comprises a cartridge seat, and a clamping groove is formed in the cartridge seat;

wherein, the draw-in groove orientation one side of PCR detection module is equipped with and is used for supplying detect in the kit liquid storage storehouse of usefulness stretches out detect the breach.

With reference to the first aspect, in one possible implementation manner, the magnetic attraction ultrasonic module includes a mounting seat, a jacking driving mechanism, an ultrasonic mechanism and a magnetic attraction mechanism;

the mounting seat is arranged in the first rack;

the ultrasonic mechanism and the magnetic attraction mechanism are movably arranged on the mounting seat through a lifting base, and an ultrasonic transduction head of the ultrasonic mechanism is upwards arranged;

the jacking driving mechanism is arranged on the mounting seat and is in transmission connection with the lifting base, and the jacking driving mechanism is used for driving the lifting base to drive the ultrasonic mechanism and the magnetic attraction mechanism to lift along the vertical direction, so that the ultrasonic transduction head and the magnetic attraction mechanism are in butt joint with the bottom of the kit.

With reference to the first aspect, in one possible implementation manner, the lifting rotation module includes a movable mounting plate, a rotation driving mechanism and a lifting driving mechanism;

the movable mounting plate is slidably arranged in the second rack along the vertical direction, and the extraction and transfer device is rotatably arranged on the movable mounting plate;

the rotary driving mechanism is arranged on the movable mounting plate and is in transmission connection with the extraction and transfer device, and the rotary driving mechanism is used for driving the extraction and transfer device to rotate; and

the lifting driving mechanism is arranged on the second frame and is in transmission connection with the movable mounting plate, and the lifting driving mechanism is used for driving the movable mounting plate to drive the extraction and transfer device to lift along the vertical direction.

With reference to the first aspect, in a possible implementation manner, the lifting driving mechanism is interposed between the rotation driving mechanism and the extraction transferring device, wherein the extraction transferring device, the lifting driving mechanism and the rotation driving mechanism are arranged along a same straight line.

With reference to the first aspect, in one possible implementation manner, the sample extraction module includes a rotating base, a pipetting mechanism and a rotational positioning mechanism;

The rotating seat is rotatably arranged on the lifting rotating module;

the pipetting mechanism is arranged on the rotating seat and is used for sucking samples or reagents for transferring;

the rotary positioning mechanism is arranged on the rotary seat and used for positioning the rotation angle of the rotary seat.

With reference to the first aspect, in one possible implementation manner, the rotary positioning mechanism includes a code wheel, an origin wheel, a first position detector, and a second position detector;

the code disc and the origin disc are both arranged on the rotating seat, and the code disc and the origin disc are coaxial with the rotating seat;

the first position detector and the second position detector are arranged on the lifting rotation module through a fixed support;

wherein the code disc is provided with a preset number of position marks, each position mark corresponds to the position of the pipetting mechanism when rotating at different angles, the origin disc is provided with an origin mark, the origin mark corresponds to the working origin position of the rotary seat, the first position detector is used for detecting the position mark, and the second position detector is used for detecting the origin mark.

In a second aspect, the present application further provides a biological sample detection method, to which the biological sample detection apparatus provided according to the first aspect is applied, the biological sample detection method including:

Placing the reagent kit loaded with the sample and the reagent on the in-out bin module;

conveying the kit to the detection station through the warehouse-in and warehouse-out module;

transferring the sample or the reagent in different liquid storage bins of the kit through the extraction and transfer device, and performing magnetic attraction and ultrasonic treatment on the sample or the reagent through the magnetic attraction ultrasonic module;

detecting the samples processed in the kit through the PCR detection module;

and sending out the kit after detection through the warehouse-in and warehouse-out module, and finishing detection.

Compared with the prior art, the beneficial effect of this application:

the application provides a biological sample check out test set up two transport detection device that make up and draw transfer device that use, carry detection device through with business turn over storehouse module, PCR detection module and magnetism to inhale ultrasonic module integration in first frame, draw transfer device through with rotatory module of lift and sample extraction module integration in the second frame. From this, biological sample check out test set will pass in and out storehouse module, PCR detection module, magnetism inhale the ultrasonic module, go up and down rotatory module and sample and draw each functional module modularization of module to with each module respectively integrate in corresponding first frame and second frame again, can carry detection device and draw the transfer device to split during the transportation, will carry detection device's first frame and draw the second frame of transfer device to assemble during the use and can use. The biological sample detection equipment provided by the application simplifies the layout of the internal structure, is simpler and more compact in structure, reduces the volume, and is more convenient to leave the factory, transport, use and assemble. And the corresponding module can be integrally disassembled and assembled during subsequent overhaul and maintenance, so that the disassembly and assembly are simpler, the workload is reduced, and the operation efficiency is greatly improved. If one module fails, the whole failed module is replaced or the whole conveying detection device and the extraction and transfer device are replaced during replacement, so that the development of detection work is not delayed.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate only some embodiments of the application and are therefore not to be considered limiting of its scope, for the purpose of providing additional related drawings from which the invention may be practiced by those of ordinary skill in the art without the exercise of inventive faculty. In the drawings:

fig. 1 is a schematic perspective view showing the structure of a biological sample detecting apparatus equipped with a kit according to the present embodiment;

FIG. 2 shows an exploded schematic view of a transport detection device and an extraction transfer device in the biological sample testing apparatus of FIG. 1;

FIG. 3 shows an exploded view of a transport detection device in the biological sample detection apparatus of FIG. 2;

FIG. 4 is a schematic perspective view showing an extraction transfer apparatus of the biological sample testing device of FIG. 2;

fig. 5 is a schematic perspective view showing a cartridge device in which a reagent cartridge is mounted in the biological sample detecting apparatus provided in the present embodiment;

FIG. 6 is a schematic view showing the structure of the cartridge device shown in FIG. 5 after the upper and lower housings are disassembled;

FIG. 7 is a schematic perspective view of a magnetic suction ultrasonic module in the conveying and detecting device shown in FIG. 3;

FIG. 8 is a schematic view showing another perspective structure of the extraction transfer apparatus shown in FIG. 4;

FIG. 9 shows an exploded view of the extraction transfer apparatus of FIG. 8;

FIG. 10 is a partial schematic view showing a three-dimensional structure of the lifting and rotating module and the sample extraction module in the extraction and transfer apparatus shown in FIG. 9;

fig. 11 is a schematic diagram showing the exploded positions of the driven pulley in the lifting rotation module and the rotary table, origin plate and code plate in the sample extraction module according to the present embodiment.

Reference numerals illustrate:

10. a transport detection device; 20. an extraction transfer device; 30. a connecting piece; 40. a kit; 41. an upper cover;

100. a first frame; 110. detecting a station; 120. a feeding station; 130. a first positioning reference surface; 140. a roller seat; 141. a roller member;

200. a bin inlet and outlet module; 210. a lifting frame; 211. a lifting arm; 212. a first slide rail and block assembly; 220. a conveying driving mechanism; 221. a first driving motor; 222. a first transmission screw; 223. a first drive nut;

300. a PCR detection module; 310. a detection port;

400. a magnetic suction ultrasonic module; 410. a mounting base; 420. a jacking driving mechanism; 421. a second driving motor; 422. a second transmission screw rod; 430. an ultrasonic mechanism; 431. an ultrasonic transducing head; 432. a fixing seat; 440. a magnetic attraction mechanism; 450. lifting a base; 460. a buffer assembly; 461. adjusting the stud; 462. a buffer spring;

500. A second frame; 510. positioning the bulge; 520. a second positioning reference surface; 530. avoidance holes;

600. a lifting rotation module; 610. a movable mounting plate; 620. a rotary driving mechanism; 621. a rotary drive assembly; 6210. a third driving motor; 6211. a motor base; 622. a mechanical transmission assembly; 6220. a driving pulley; 6221. a driven pulley; 6222. a drive belt; 630. a lifting driving mechanism; 631. a fourth driving motor; 632. a third transmission screw rod; 633. a third drive nut; 640. a guide mechanism; 641. a guide seat; 642. a second slide rail and block assembly; 650. a limit detector; 660. an induction piece;

700. a sample extraction module; 710. a rotating seat; 711. a toggle rod; 720. a pipetting mechanism; 721. a transfer pump; 722. a liquid suction nozzle; 730. a rotary positioning mechanism; 731. a code wheel; 7310. a position mark; 732. an origin plate; 7320. an origin mark; 733. a first position detector; 734. a second position detector; 740. a fixed bracket; 750. a collecting ring;

800. a cartridge device; 810. a cartridge holder; 811. a clamping groove; 8110. detecting the notch; 8111. a through hole; 812. an upper housing; 813. a lower housing; 820. a compressing mechanism; 821. a pressing plate; 8210. a wedge surface; 822. an elastic reset piece; 830. a clamping mechanism; 840. an in-place detection mechanism; 850. heating the positioning seat;

X, width direction; y, length direction; z, vertical direction.

Detailed Description

The following describes in detail the implementation of the embodiments of the present application with reference to the accompanying drawings. It should be understood that the detailed description is presented herein by way of illustration and explanation of the present application examples, and is not intended to limit the present application examples.

It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be combined with each other.

In the embodiments of the present application, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "axial," "radial," "circumferential," and the like indicate an orientation or positional relationship based on that shown in the drawings, merely for convenience of description and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

In this application, unless specifically stated and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art as the case may be.

The present application will be described in detail below with reference to the attached drawings in conjunction with exemplary embodiments.

Examples

Referring to fig. 1 and 2, the present embodiment provides a biological sample detection apparatus for detecting a sample, specifically, detecting a nucleic acid by using PCR (Polymerase Chain Reaction ) technology.

In the present embodiment, the biological sample detecting apparatus includes the conveyance detecting device 10 and the extraction transferring device 20, the conveyance detecting device 10 and the extraction transferring device 20 are disposed in order in the vertical direction Z, and detachable connection is made between the conveyance detecting device 10 and the extraction transferring device 20. That is, the conveying detection device 10 and the extraction and transfer device 20 can be disassembled and individually packaged for transportation, and when the conveying detection device 10 and the extraction and transfer device 20 are required to be assembled for use, the conveying detection device can be used.

Referring to fig. 3 and fig. 4, the conveying and detecting device 10 includes a first frame 100, and a bin-in/out module 200, a PCR detecting module 300, and a magnetic-attraction ultrasonic module 400 disposed on the first frame 100. The first frame 100 is further provided with a detection station 110, and a feeding station 120 is arranged outside the first frame 100. The in-out bin module 200 is provided with a clamping part for clamping the reagent kit 40, and the in-out bin module 200 is used for driving the clamping part to transfer between the detection station 110 and the feeding station 120 outside the first frame 100, so that the reagent kit 40 installed on the clamping part is transferred from the feeding station 120 to the detection station 110 for extraction, transfer, uniform mixing and other pretreatment detection procedures.

Wherein the kit 40 is provided with a plurality of reservoirs (not shown) for storing samples or reagents, each reservoir being adapted to store a different sample or reagent. Optionally, a plurality of reservoirs are distributed about the axis of the kit 40. In some embodiments, the plurality of reservoirs are evenly distributed about the axis of the kit 40, or may be spaced apart by a predetermined angle.

The magnetic suction ultrasonic module 400 and the PCR detection module 300 are both arranged corresponding to the detection station 110, wherein the magnetic suction ultrasonic module 400 is used for performing magnetic suction and ultrasonic treatment on samples in corresponding liquid storage bins, and the PCR detection module 300 is used for detecting the samples in the corresponding liquid storage bins. In this embodiment, the PCR detection module 300 is used for optical detection (e.g. fluorescence detection) of a sample.

The extraction and transfer apparatus 20 includes a second frame 500, a lifting and rotating module 600, and a sample extraction module 700. The second rack 500 is stacked above the first rack 100, and the second rack 500 is detachably connected to the first rack 100. The lifting rotary module 600 is disposed in the second rack 500, the sample extraction module 700 is disposed above the detection station 110 and above the lifting rotary module 600, and the lifting rotary module 600 is used for driving the sample extraction module 700 to lift and rotate, so that the sample extraction module 700 can absorb sample transfer in a corresponding liquid storage bin, specifically, transfer from one liquid storage bin to another liquid storage bin.

Referring to fig. 2, 3 and 4, two positioning protrusions 510 are disposed at the bottom of the second frame 500, two ends of the positioning protrusions 510 extend along the length direction Y of the second frame 500, and the width of the two positioning protrusions 510 is adapted to the width between the inner walls of the two side plates of the first frame 100. That is, when the second chassis 500 is assembled with the first chassis 100, the positioning protrusion 510 abuts against the inner wall of the side plate, thereby realizing the mounting and positioning of the second chassis 500 in the width direction X.

Further, when the first frame 100 is assembled with the second frame 500, the positioning protrusion 510 is abutted against the inner wall of the side plate, and then the first positioning reference surface 130 is aligned with the second positioning reference surface 520, so that the second frame 500 is installed and positioned along the length direction Y. Thus, by positioning the width direction X and the length direction Y of the second frame 500, the second frame 500 can be accurately mounted on the first frame 100, ensuring that the conveyance detecting device 10 and the extraction transfer device 20 operate normally.

A connecting piece 30 is further arranged between the first frame 100 and the second frame 500, the connecting piece 30 comprises a first connecting portion and a second connecting portion which are integrally connected, planes of the first connecting portion and the second connecting portion are perpendicular to each other, and optionally, the connecting piece 30 is an inverted L-shaped right-angle block. Wherein the first connection part is detachably connected with the side plate of the first frame 100, and the second connection part is detachably connected with the bottom of the second frame 500 by a fastener (e.g., a bolt or a screw). It will also be appreciated that the second connection can also provide support for the second housing 500.

In this embodiment, two connecting members 30 are provided, and the two connecting members 30 are distributed along the width direction X of the second frame 500, so that the connection between the first frame 100 and the second frame 500 can be more stable by adopting two connecting members 30.

Compared with the prior art, the biological sample detection device provided in this embodiment is provided with two conveying detection devices 10 and an extraction transfer device 20, wherein the conveying detection devices 10 are integrated on the first rack 100 through the in-out bin module 200, the PCR detection module 300 and the magnetic suction ultrasonic module 400, and the extraction transfer device 20 is integrated on the second rack 500 through the lifting rotation module 600 and the sample extraction module 700. Therefore, the biological sample detection device modularizes the functional components of the warehouse entry module 200, the PCR detection module 300, the magnetic suction ultrasonic module 400, the lifting rotation module 600 and the sample extraction module 700, integrates the modules in the corresponding first rack 100 and second rack 500 respectively, can split the conveying detection device 10 and the extraction transfer device 20 during transportation, and can be used by assembling the first rack 100 of the conveying detection device 10 and the second rack 500 of the extraction transfer device 20 during use. Therefore, the biological sample detection equipment provided by the embodiment simplifies the layout of the internal structure, is simpler and more compact in structure, reduces the volume, and is more convenient to leave the factory, transport, use and assemble. And the corresponding module can be integrally disassembled and assembled during subsequent overhaul and maintenance, so that the disassembly and assembly are simpler, the workload is reduced, and the operation efficiency is greatly improved.

In addition, if one of the modules fails, the entire failed module is replaced or the entire transport inspection apparatus 10 and the pick-up transfer apparatus 20 are replaced during replacement, so that the inspection work is not delayed.

Referring to fig. 2 and 3, in this embodiment, the in-out bin module 200 includes a lifting frame 210 and a conveying driving mechanism 220. The lifting frame 210 is slidably disposed on the first frame 100, and the clamping portion is disposed on the lifting frame 210. The conveying driving mechanism 220 is disposed on the first frame 100 and is in transmission connection with the lifting frame 210, and the conveying driving mechanism 220 is used for driving the lifting frame 210 to drive the clamping portion to transfer between the detecting station 110 and the loading station 120.

The lifting frame 210 has two lifting arms 211 arranged in parallel, the two lifting arms 211 are respectively arranged at two sides of the first frame 100, and the two lifting arms 211 are slidably matched with the first frame 100 through a first sliding rail and sliding block assembly 212. The first sliding rail and sliding block assembly 212 includes a first guiding sliding rail disposed on the lifting arm 211 and a first guiding sliding block disposed on an outer wall of the side plate of the first rack 100, where the first guiding sliding rail extends along the length direction Y of the lifting arm 211, and the first guiding sliding block is in sliding fit with the first guiding sliding rail.

The transport driving mechanism 220 may output a linear reciprocating motion, and thus, the transport driving mechanism 220 may be selected as a cylinder, an oil cylinder, an electric push rod, a linear motor, a screw motor, or a linear module. It is to be understood that the foregoing is illustrative only and is not to be construed as limiting the scope of the present application.

In this embodiment, as shown in fig. 2, the conveying driving mechanism 220 is selected to be a screw motor, and includes a first driving motor 221, a first transmission screw 222 and a first transmission nut 223, where the first driving motor 221 is disposed at one end of the first frame 100 far away from the detection station 110 along the length direction Y of the first frame 100, the first transmission screw 222 is rotatably disposed on the first frame 100, and two ends of the first transmission screw 222 extend toward the length direction Y of the first frame 100, where the first transmission screw 222 is connected with a motor shaft of the first driving motor 221. The first transmission nut 223 is sleeved on the first transmission screw rod 222 and is matched with the transmission screw rod in a threaded pair, and the first transmission nut 223 is connected with the lifting arm 211 positioned on the same side of the first frame 100. Therefore, the first driving screw rod 222 is driven to rotate by the first driving motor 221, and then the first driving screw rod 222 drives the first driving nut 223 to drive the lifting arm 211 to move between the feeding station 120 and the detecting station 110.

When the lifting arm 211 moves to the feeding station 120, the lifting arm 211 is in a cantilever structure, and the reagent kit 40 is mounted on the clamping portion of the lifting arm 211 far away from the first rack 100, so that the lifting arm 211 bears a large bending moment, and the whole biological sample detection device is at a tilting risk. To solve this problem, in this embodiment, the first driving motor 221 is disposed at an end of the first frame 100 away from the detection station 110, that is, the first driving motor 221 with a heavy mass is disposed behind the first frame, so that the center of gravity of the whole conveying driving mechanism 220 can be transferred to an end of the first frame 100 away from the detection station 110, so as to offset the bending moment received by the lifting arm 211, thereby improving the stability of the motion of the whole conveying driving mechanism 220 and ensuring the reliability of the biological sample detection device. Alternatively, the first driving motor 221 may be a stepping motor or a servo motor, which is more accurate in control.

Referring to fig. 2, 3, 5 and 6, further, the clamping portion is provided with a cartridge device 800, and the cartridge device 800 includes a cartridge holder 810, a pressing mechanism 820, a clamping mechanism 830 and an in-place detecting mechanism 840. The cartridge holder 810 is disposed at one end of the lifting frame 210 of the in-out module 200 away from the first rack 100, and the cartridge holder 810 is provided with a clamping groove 811 for accommodating the reagent cartridge 40. The hold down mechanism 820 can be moved toward or away from the slot 811 to clamp or unclamp the cartridge 40. The positioning mechanism 830 protrudes from the side wall of the clamping groove 811 into the clamping groove 811 to position the reagent cartridge 40. The in-place detection mechanism 840 is used for detecting the reagent box 40 in the clamping groove 811 and sending an in-place signal of the reagent box 40 to the in-out bin module 200, and when the in-out bin module 200 receives the in-place signal, the conveying driving mechanism 220 drives the lifting frame 210 to drive the card box device 800 to move from the feeding station 120 to the detection station 110.

Referring to fig. 3, 5 and 6, a detection notch 8110 is provided on a side of the card slot 811 facing the PCR detection module 300, where the liquid storage bin for detection extends. The PCR detection module 300 is provided with a detection port 310 for inserting the liquid storage bin corresponding to the detection notch 8110.

The cartridge holder 810 includes an upper housing 812 and a lower housing 813, the upper housing 812 and the lower housing 813 are combined together by a snap-fit or screw connection, and a mounting cavity (not shown) for mounting the pressing mechanism 820 is formed between the upper housing 812 and the lower housing 813. The cartridge seat 810 is composed of an upper shell 812 and a lower shell 813, so that on one hand, structural components in the installation cavity can be protected, and on the other hand, a packaging effect is achieved, so that the whole cartridge seat 810 is more attractive.

The pressing mechanisms 820 are provided in two, the two pressing mechanisms 820 are distributed along the width direction X of the first frame 100, and the two pressing mechanisms 820 are movable in the radial direction of the clamping groove 811. Each pressing mechanism 820 comprises a pressing plate 821 and an elastic reset piece 822, wherein the pressing plate 821 is slidably arranged in the mounting cavity, the elastic reset piece 822 is arranged in the mounting cavity, and the elastic reset piece 822 is used for driving the pressing plate 821 to move in a direction away from the clamping groove 811. Alternatively, the resilient return 822 is selected to be a compression spring.

Referring to fig. 3, 5 and 6, further, the pressing plate 821 at least partially extends out of the cartridge holder 810, and an end surface of the pressing plate 821 away from the cartridge holder 810 is a wedge-shaped surface 8210, two sides of the corresponding first frame 100 are further provided with roller holders 140 in press fit with the pressing plate 821, the roller holders 140 are arranged corresponding to the detection stations 110, and a plurality of roller members 141 are disposed on the roller holders 140 along a moving direction of the cartridge holder 810. Thus, when the lifting arm 211 drives the cartridge holder 810 to move to the detecting station 110, the roller member 141 presses the pressing plate 821 to move the pressing plate 821 toward the clamping groove 811 so as to clamp the reagent cartridge 40 in the clamping groove 811.

The clamping mechanism 830 is a limiting boss, and a corresponding kit 40 is provided with a limiting groove matched with the limiting boss in a concave-convex manner. Alternatively, the number of the limiting bosses may be multiple, and the limiting bosses are distributed at intervals along the inner wall surface of the clamping groove 811.

The in-place detection mechanism 840 is a photoelectric sensor, and a light shielding block matched with the photoelectric sensor is arranged on the kit 40. Alternatively, in some embodiments, the in-place detection mechanism 840 is a proximity switch or travel switch, and the kit 40 is provided with a mating sensing element.

The cartridge device 800 further comprises a heating plate (not shown) and a heating positioning seat 850, the heating plate is disposed at the bottom of the slot 811, the heating positioning seat 850 is covered on the heating plate, the heating positioning seat 850 is a bump, and a heating positioning groove (not shown) matched with the heating positioning seat 850 in a concave-convex manner is disposed at the bottom of the corresponding kit 40. Wherein, the heating positioning seat 850 is made of a heat conductive material.

Referring to fig. 2, 3 and 7, the magnetic ultrasonic module 400 is disposed directly below the detection station 110, and the magnetic ultrasonic module 400 includes a mounting base 410, a lifting driving mechanism 420, an ultrasonic mechanism 430 and a magnetic mechanism 440. Wherein, the mounting seat 410 is disposed in the first frame 100, the ultrasonic mechanism 430 and the magnetic attraction mechanism 440 are movably disposed on the mounting seat 410 through the lifting base 450, and the ultrasonic transducing head 431 of the ultrasonic mechanism 430 is disposed upwards.

The lifting driving mechanism 420 is disposed on the mounting base 410 and is in transmission connection with the lifting base 450, and a through hole 8111 (refer to fig. 6) for the ultrasonic transducer 431 to pass through is disposed at the bottom of the clamping groove 811 of the clamping box base 810. When the cartridge seat 810 drives the reagent cartridge 40 to move to the detection station 110, the lifting driving mechanism 420 drives the lifting base 450 to drive the ultrasonic mechanism 430 and the magnetic attraction mechanism 440 to lift along the vertical direction Z, so that the ultrasonic transduction head 431 and the magnetic attraction mechanism 440 are abutted to the bottom of the reagent cartridge 40, and the ultrasonic transduction head 431 and the magnetic attraction mechanism 440 respectively correspond to respective liquid storage bins, and the ultrasonic transduction head 431 and the magnetic attraction mechanism 440 are used for carrying out ultrasonic and magnetic attraction treatment on the respective liquid storage bins. Thus, the ultrasonic mechanism 430 and the magnetic attraction mechanism 440 are assembled on the lifting base 450 and driven by the same lifting driving mechanism 420, so as to avoid the energy dissipation of the ultrasonic mechanism 430.

Further, the ultrasonic mechanism 430 is disposed on the lifting base 450 through a buffer assembly 460, wherein the buffer assembly 460 can absorb a reaction force generated by the ultrasonic transducer 431 abutting against the bottom of the kit 40, so as to protect the ultrasonic transducer 431; on the other hand, the buffer assembly 460 may also maintain the preset abutment force between the ultrasonic transducer 431 and the kit 40, thereby ensuring the effect of ultrasonic treatment, and preventing the abutment force from being too large, so that the kit 40 is damaged or is separated from the clamping groove 811.

In this embodiment, a plurality of buffer assemblies 460 are provided, the plurality of buffer assemblies 460 are disposed around the ultrasound mechanism 430, the buffer assemblies 460 include an adjusting stud 461 and a buffer spring 462 sleeved on the adjusting stud 461, the adjusting stud 461 penetrates through the ultrasound fixing seat 432 on which the ultrasound mechanism 430 is mounted and is in threaded connection with the lifting base 450, one end of the buffer spring 462 abuts against the ultrasound fixing seat 432, and the other end abuts against the lifting base 450. Thus, the adjusting stud 461 is provided with a guiding function, and the amount of compression of the buffer spring 462 can be adjusted by screwing the adjusting stud 461, so that the amount of abutment force generated when the ultrasonic transducer 431 abuts against the reagent cartridge 40 can be adjusted.

Optionally, the jacking driving mechanism 420 is selected to be a penetrating screw motor, where the jacking driving mechanism 420 includes a second driving motor 421 and a second transmission screw 422, the second transmission screw 422 is fixedly installed on the installation seat 410, the second driving motor 421 is internally provided with a second transmission nut (not shown) matched with the second transmission screw 422, the second transmission screw 422 penetrates the second driving motor 421, and the second driving motor 421 is connected with the lifting base 450. Therefore, the second driving motor 421 can be started to lift along the vertical direction Z, so as to drive the lifting base 450 to lift together, thereby obtaining a larger lifting stroke in a limited space and being more compact in structure. Of course, in some embodiments, the lifting driving mechanism 420 may be a cylinder, an oil cylinder, a linear motor, an electric push rod, or the like.

Referring to fig. 2, 8, 9 and 10, the lifting/rotating module 600 includes a movable mounting plate 610, a rotation driving mechanism 620 and a lifting driving mechanism 630. The movable mounting plate 610 is slidably disposed in the second frame 500 along the vertical direction Z, and the extraction transfer apparatus 20 is rotatably disposed on the movable mounting plate 610. The rotary driving mechanism 620 is disposed on the movable mounting plate 610 and is in transmission connection with the extraction and transfer device 20, and the rotary driving mechanism 620 is used for driving the extraction and transfer device 20 to rotate. The lifting driving mechanism 630 is disposed on the second frame 500 and is in transmission connection with the movable mounting plate 610, and the lifting driving mechanism 630 is used for driving the movable mounting plate 610 to drive the extraction and transfer device 20 to lift along the vertical direction Z. Since the rotary driving mechanism 620 is disposed on the movable mounting plate 610, the rotary driving mechanism 620 can also be lifted and lowered synchronously with the movable mounting plate 610.

In the present embodiment, the lift drive mechanism 630 is interposed between the rotation drive mechanism 620 and the extraction transfer apparatus 20, wherein the extraction transfer apparatus 20, the lift drive mechanism 630, and the rotation drive mechanism 620 are arranged along the same straight line. Thereby making reasonable use of the space between the extraction transfer device 20 and the rotation driving mechanism 620, making the lifting rotation module 600 more compact in overall structure.

Further, the lifting rotation module 600 further includes two guiding mechanisms 640, the two guiding mechanisms 640 are respectively disposed on two inner sidewalls of the second frame 500, and the two guiding mechanisms 640 are symmetrically disposed. The movable mounting plate 610 is disposed on two guide mechanisms 640, and the guide mechanisms 640 are used for providing guide for lifting of the movable mounting plate 610 so as to enable the movable mounting plate 610 to lift more stably.

Each guide mechanism 640 includes a guide holder 641 and a second sliding rail assembly 642, and the guide holder 641 is slidably disposed on the second frame 500 through the second sliding rail assembly 642. Wherein, the movable mounting plate 610 and the guide holder 641 are detachably connected through fasteners. Optionally, the fastener is a screw or bolt.

In this embodiment, two second sliding rail assemblies 642 are disposed in each guiding mechanism 640 to ensure the sliding stability. Further, the second sliding rail and sliding block assembly 642 includes a second guiding sliding rail and a second guiding sliding block arranged along the vertical direction Z, the second guiding sliding rail is disposed on the second frame 500 and extends along the vertical direction Z, and the second guiding sliding block is slidably disposed on the second guiding sliding rail and connected with the guiding seat 641.

Further, the at least one guiding mechanism 640 further includes at least two limit detectors 650, the two limit detectors 650 are disposed on the second frame 500 at intervals along the vertical direction Z, and the guiding seat 641 of the guiding mechanism 640 is provided with a sensing piece 660 in sensing fit with the limit detectors 650. Thus, the maximum travel of the movable mounting plate 610 moving in the vertical direction Z can be effectively limited by the two limit detectors 650.

In some embodiments, limit detector 650 is selected to be a photosensor and sensing piece 660 is selected to be a light blocking piece, thereby blocking the impinging light beam in the photosensor by the light blocking piece, thereby triggering the photosensor.

In other embodiments, limit detector 650 is selected to be a pair of travel switches and sensing piece 660 is selected to be a touch pad, whereby the travel switches are bumped by the touch pad, triggering the travel switches.

In still other embodiments, limit detector 650 may be selected as a proximity sensor and sensing piece 660 may be selected as a metallic piece, thereby triggering the proximity sensor by the metallic piece approaching the proximity sensor.

It is to be understood that the foregoing is illustrative only and is not to be construed as limiting the scope of the present application.

Referring to fig. 2, 8, 9 and 10, the rotary driving mechanism 620 includes a rotary driving assembly 621 and a mechanical transmission assembly 622, the rotary driving assembly 621 is disposed on the movable mounting plate 610, and the rotary driving assembly 621 is in transmission connection with the extraction and transfer device 20 through the mechanical transmission assembly 622 for driving the extraction and transfer device 20 to rotate around its own axis.

The rotary driving unit 621 is a third driving motor 6210, and the third driving motor 6210 is mounted on the movable mounting plate 610 upside down by a motor mount 6211. The mechanical drive assembly 622 may alternatively be a gear drive assembly, a sprocket drive assembly, or a pulley drive assembly. Alternatively, the third drive motor 6210 may be selected as a servo motor or a stepper motor, which is more accurate in control.

In the present embodiment, the mechanical transmission assembly 622 is a belt transmission assembly, which includes a driving pulley 6220, a driven pulley 6221, and a transmission belt 6222, the driving pulley 6220 is connected with a motor shaft of the third driving motor 6210, the driven pulley 6221 is disposed on the extraction transfer device 20, and the transmission belt 6222 is sleeved between the driving pulley 6220 and the driven pulley 6221. Thus, the third driving motor 6210 drives the driving pulley 6220 to rotate, and the driven pulley 6221 is driven by the driving belt 6222 to rotate the extraction transfer apparatus 20. Wherein the lifting drive mechanism 630 is interposed between the driving pulley 6220 and the driven pulley 6221. Alternatively, the driving pulley 6220 and the driven pulley 6221 may be selected as timing pulleys and the drive belt 6222 may be selected as timing belt. Further, an avoidance hole 530 for avoiding the third driving motor 6210 is formed at the top of the second frame 500, so as to avoid interference with the second frame 500 when the third driving motor 6210 is lifted.

The lifting driving mechanism 630 may output a linear reciprocating motion, wherein the lifting driving mechanism 630 may be selected from a cylinder, an oil cylinder, an electric push rod, a linear motor, a screw motor, or a linear module.

In the present embodiment, the elevation driving mechanism 630 is selected as a screw motor, wherein the elevation driving mechanism 630 includes a fourth driving motor 631, a third transmission screw 632, and a third transmission nut 633, the fourth driving motor 631 is disposed at the top of the second housing 500, and a motor shaft of the fourth driving motor 631 is directed downward in the vertical direction Z. One end of the third transmission screw 632 is connected with a motor shaft of the fourth driving motor 631 through a coupling (not shown), and the other end of the third transmission screw 632 extends in the vertical direction Z and is rotatably engaged with the bottom of the second frame 500. The third transmission nut 633 is sleeved on the third transmission screw 632 and is matched with the thread pair of the third transmission screw 632, and the third transmission nut 633 is connected with the movable mounting plate 610 through a fastener (such as a bolt and the like). Thereby, the third transmission screw 632 is driven to rotate in the forward and reverse directions by the fourth driving motor 631, so that the third transmission nut 633 is driven to drive the movable mounting plate 610 to perform lifting movement in the vertical direction Z. Alternatively, the fourth driving motor 631 may be selected as a servo motor or a stepping motor, which is more accurate in control.

Referring to fig. 2, 8, 9 and 10, the sample extraction module 700 includes a rotating base 710, a pipetting mechanism 720 and a rotational positioning mechanism 730. The rotating base 710 is rotatably disposed on the movable mounting plate 610 of the lifting rotating module 600, and the pipetting mechanism 720 is disposed on the rotating base 710, where the pipetting mechanism 720 is used for sucking samples or reagents into corresponding reservoirs for transferring. The rotation positioning mechanism 730 is disposed on the rotating base 710, and is used for positioning a rotation angle of the rotating base 710, thereby improving rotation accuracy of the rotating base 710.

Further, the rotating seat 710 is provided with the driven pulley 6221, and the driven pulley 6221 is provided coaxially with the rotating seat 710. Thus, rotation of the driven pulley 6221 drives rotation of the rotary seat 710 about its own axis.

In some embodiments, the driven pulley 6221 is integrally formed with the rotary seat 710, i.e., the driven pulley 6221 is machined into the rotary seat 710.

In other embodiments, the driven pulley 6221 and the rotating seat 710 are manufactured separately, and the driven pulley 6221 and the rotating seat 710 are coupled together by fasteners (e.g., bolts, etc.) or welding.

Referring to fig. 11, the rotary positioning mechanism 730 includes a code wheel 731, an origin wheel 732, a first position detector 733, and a second position detector 734. Wherein, code wheel 731 and origin wheel 732 are both disposed on rotary base 710, and code wheel 731 and origin wheel 732 are coaxial with rotary base 710. In the present embodiment, the rotary seat 710, the driven pulley 6221, the origin disc 732, and the code disc 731 are sequentially stacked in the vertical direction Z. Of course, in some embodiments, the rotary seat 710, the driven pulley 6221, the origin disc 732, and the code disc 731 may be arranged in other sequences. It is to be understood that the foregoing is illustrative only and is not to be construed as limiting the scope of the present application.

The first position detector 733 and the second position detector 734 are arranged on the lifting rotary module 600 through the fixing support 740, and the collecting ring 750 is further arranged on the fixing support 740, and the collecting ring 750 is used for supplying power to the pipetting mechanism 720, so that wire interference when the pipetting mechanism 720 rotates along with the rotary seat 710 is avoided.

The code disc 731 is provided with a preset number of position identifiers 7310, each position identifier 7310 corresponds to a position of the pipetting mechanism 720 when rotated by different angles, the origin disc 732 is provided with an origin identifier 7320, the origin identifier 7320 corresponds to a working origin position of the rotating seat 710, the first position detector 733 is used for detecting the position identifier 7310, and the second position detector 734 is used for detecting the origin identifier 7320.

Thereby, the position mark 7310 is detected by the first position detector 733, thereby accurately controlling the rotation angle of the rotating base 710. After the rotary driving mechanism 620 drives the rotary base 710 to drive the pipetting mechanism 720 to perform the complete set of procedures, the rotary driving mechanism 620 drives the origin disc 732 to rotate (either reversely or positively) again, so that the second position detector 734 detects the origin mark 7320, and thus the rotary base 710 is judged to return to the initial position, and the rotary base 710 is stopped at the initial position for preparing for the next action, thereby eliminating errors generated in the rotation process and improving the precision of each rotation of the rotary base 710.

Alternatively, the first position detector 733 and the second position detector 734 may each be selected as a photoelectric sensor, the corresponding position mark 7310 and origin mark 7320 may be designed as a light-transmitting notch or through hole 8111, or the corresponding position mark 7310 and origin mark 7320 may be designed as light-shielding teeth.

Alternatively, a predetermined number of the position indicators 7310 may be distributed about the axis of the code wheel 731 and in a one-to-one correspondence with the reservoirs in the kit 40.

In some embodiments, code wheel 731 and origin wheel 732 are a unitary structure. In other embodiments, the code wheel 731 and the origin wheel 732 are separate pieces, and the code wheel 731 and the origin wheel 732 are coupled together by fasteners (e.g., bolts, etc.) or welding.

The pipetting mechanism 720 can be lifted and rotated along with the rotating seat 710, the pipetting mechanism 720 comprises a pipetting pump 721 and a pipetting nozzle 722 arranged on the pipetting pump 721, and the pipetting nozzle 722 faces downwards along the vertical direction Z and is used for extending into or withdrawing from the liquid storage bin to aspirate a sample or a reagent for transferring.

Further, in some embodiments, a toggle lever 711 is disposed below the rotating base 710, and the toggle lever 711 is eccentrically mounted on a side of the rotating base 710 facing the detecting station 110, and can rotate and lift with the rotating base 710. Thereby, the toggle lever 711 is moved along the vertical direction Z with the rotation base 710 to a position to be inserted into or withdrawn from the toggle hole on the upper cover 41 of the kit 40. When the toggle lever 711 is inserted into the toggle hole, the toggle lever 711 can drive the upper cover 41 to rotate along with the rotating seat 710 (the body of the kit 40 is limited by the pressing plate 821 and the clamping mechanism 830 and does not rotate along with the upper cover 41). Therefore, the upper cover 41 is driven to rotate along with the rotating seat 710 by the toggle rod 711, so that the liquid suction nozzle 722 can be aligned with any liquid storage bin in the vertical direction Z, and the sucking and transferring of samples or reagents can be realized.

Referring to fig. 1 to 11, the present embodiment further provides a biological sample detection method, to which the biological sample detection apparatus provided above is applied, the biological sample detection method includes the following steps:

s100: placing a reagent kit 40 loaded with a sample and a reagent on the in-out bin module 200;

s200: the reagent box 40 is conveyed to the detection station 110 through the warehouse-in and warehouse-out module 200;

s300: transferring the sample or the reagent in different liquid storage bins of the kit 40 through the extraction and transfer device 20, and performing magnetic attraction and ultrasonic treatment on the sample or the reagent through the magnetic attraction ultrasonic module 400; further, in the present embodiment, the sample or the reagent may be heat-treated by the heat patch.

S400: detecting the sample processed in the kit 40 by the PCR detection module 300;

s500: the kit 40 with the detection is sent out through the in-out bin module 200, and the detection is completed.

Referring to the drawings, the working principle of the biological sample detection device provided in the specific embodiment is as follows:

(1) The in-out bin module 200 drives the cartridge device 800 to move to the feeding station 120, consumables of the special reagent cartridge 40 filled with samples and reagents to be detected are placed into a clamping groove 811 of the cartridge device 800, a position detection mechanism at the bottom of the cartridge device 800 detects that the reagent cartridge 40 is placed into the clamping groove 811, and then the in-out bin module 200 drives the cartridge device 800 to move to the detection station 110 to wait for the next process.

(2) The lifting rotary module 600 drives the sample extraction module 700 to take a pipette tip from the reagent kit 40 (a special pipette tip is arranged in the reagent kit 40), the rotary driving mechanism 620 in the lifting rotary module 600 can drive the pipette tip 722 in the sample extraction module 700 to rotate until any liquid storage bin of the reagent kit 40 is aligned in the vertical direction Z, the lifting driving mechanism 630 is matched to drive the sample extraction module 700 to lift, and then the pipette tip 721 is matched to absorb and discharge liquid to sequentially transfer samples or reagents in the reagent kit 40 and mix the samples or reagents uniformly; the magnetic suction ultrasonic module 400 can respectively perform magnetic suction and ultrasonic treatment on the liquid in the corresponding liquid storage bin, and the liquid-transferring pump 721 takes the liquid-transferring gun head back to the original position of the kit 40 after the steps before each detection are completed; the nucleic acid sample is then pressed into a reservoir for detection at the plunger hole site of the kit 40. Finally, the cartridge 40 is driven by the cartridge in-out module 200 to insert the liquid cartridge for detection into the detection port 310 of the PCR detection module 300 for amplification, optical detection and analysis of the result.

(3) After the detection is completed, the warehouse entry and exit module 200 drives the kit 40 to move to the feeding station 120, and an experimenter takes away the kit 40 for innocent treatment, so that the whole detection flow is completed.

The foregoing details of the optional implementation manner of the embodiment of the present application have been described in detail with reference to the accompanying drawings, but the embodiment of the present application is not limited to the specific details of the foregoing implementation manner, and various simple modifications may be made to the technical solution of the embodiment of the present application within the scope of the technical concept of the embodiment of the present application, and these simple modifications all belong to the protection scope of the embodiment of the present application.

Moreover, any combination of the various embodiments of the present application may be made, so long as it does not deviate from the idea of the embodiment of the present application, and it should also be regarded as the disclosure of the embodiment of the present application.

Claims (12)

1. A biological sample testing device for testing a sample, the biological sample testing device comprising:

the conveying detection device (10) comprises a first rack (100), a warehouse-in and warehouse-out module (200), a PCR detection module (300) and a magnetic suction ultrasonic module (400) which are arranged on the first rack (100), wherein a detection station (110) is further arranged on the first rack (100), the warehouse-in and warehouse-out module (200) is used for conveying a kit (40) loaded with samples and reagents to the detection station (110), the magnetic suction ultrasonic module (400) and the PCR detection module (300) are respectively arranged corresponding to the detection station (110), the magnetic suction ultrasonic module (400) is used for carrying out magnetic suction and ultrasonic treatment on the samples or the reagents in the kit (40), and the PCR detection module (300) is used for detecting the samples processed in the kit (40); and

Draw transfer device (20), including second frame (500), lift rotatory module (600) and sample extraction module (700), second frame (500) detachably set up in first frame (100), lift rotatory module (600) set up in second frame (500), sample extraction module (700) set up in lift rotatory module (600) and be located the top of detecting station (110), lift rotatory module (600) are used for the drive sample extraction module (700) go up and down and rotate, so that sample extraction module (700) absorb sample or reagent in kit (40) shifts.

2. The biological sample detection device according to claim 1, wherein the bottom of the second rack (500) is provided with two positioning protrusions (510), both ends of the positioning protrusions (510) extend along the length direction (Y) of the second rack (500), and the width of the two positioning protrusions (510) is adapted to the width between the inner walls of the two side plates of the first rack (100);

wherein, first frame (100) is close to along length direction (Y) an end face of detecting station (110) is first location reference surface (130), second frame (500) is close to along length direction (Y) an end face of detecting station (110) is second location reference surface (520), when first frame (100) with second frame (500) assembly, location protruding (510) with the inner wall butt of curb plate, and first location reference surface (130) with second location reference surface (520) are aligned.

3. The biological sample detection device according to claim 1, wherein a connecting piece (30) is arranged between the first rack (100) and the second rack (500), the connecting piece (30) comprises a first connecting portion and a second connecting portion which are integrally connected, planes of the first connecting portion and the second connecting portion are perpendicular to each other, the first connecting portion is detachably connected with a side plate of the first rack (100), and the second connecting portion is detachably connected with a bottom of the second rack (500).

4. The biological sample detection device according to claim 1, wherein the in-out bin module (200) is provided with a clamping portion for clamping the reagent kit (40), the reagent kit (40) is provided with a plurality of liquid storage bins for storing samples or reagents, and the in-out bin module (200) is used for driving the clamping portion to transfer between the detection station (110) and a feeding station (120) outside the first rack (100).

5. The biological sample testing device of claim 1, wherein the in-out cartridge module (200) comprises a lifting frame (210) and a transport drive mechanism (220);

the lifting frame (210) is slidably arranged on the first rack (100), and a clamping part for clamping the reagent box (40) is arranged on the lifting frame (210);

The conveying driving mechanism (220) is arranged on the first frame (100) and is in transmission connection with the lifting frame (210), and the conveying driving mechanism (220) is used for driving the lifting frame (210) to drive the clamping part to approach or separate from the detection station (110) along the length direction (Y) of the first frame (100).

6. The biological sample detection apparatus according to claim 4 or 5, wherein the clamping portion is provided with a cartridge device (800), the cartridge device (800) comprising a cartridge holder (810), a pressing mechanism (820), a detent mechanism (830) and an in-place detection mechanism (840);

the cartridge seat (810) is arranged on the warehouse-in and warehouse-out module (200), and the cartridge seat (810) is provided with a clamping groove (811) for accommodating the reagent cartridge (40);

the pressing mechanism (820) can be close to or far away from the clamping groove (811) for clamping or loosening the kit (40);

the clamping mechanism (830) protrudes from the side wall of the clamping groove (811) into the clamping groove (811) and is used for positioning the kit (40);

the in-place detection mechanism (840) is used for detecting the kit (40) in the clamping groove (811) and sending an in-place signal of the kit (40) to the in-out bin module (200).

7. The biological sample detection apparatus according to claim 4 or 5, wherein the clamping portion is provided with a cartridge device (800), the cartridge device (800) comprises a cartridge seat (810), and a clamping groove (811) is formed in the cartridge seat (810);

Wherein, a detection notch (8110) used for extending out of a liquid storage bin for detection in the kit (40) is arranged on one side of the clamping groove (811) facing the PCR detection module (300).

8. The biological sample detection device of claim 1, wherein the magnetically-attractable ultrasonic module (400) comprises a mount (410), a jacking drive mechanism (420), an ultrasonic mechanism (430), and a magnetically-attractable mechanism (440);

the mounting seat (410) is arranged in the first rack (100);

the ultrasonic mechanism (430) and the magnetic attraction mechanism (440) are movably arranged on the mounting seat (410) through a lifting base (450), and an ultrasonic transduction head (431) of the ultrasonic mechanism (430) is arranged upwards;

the jacking driving mechanism (420) is arranged on the mounting seat (410) and is in transmission connection with the lifting base (450), the jacking driving mechanism (420) is used for driving the lifting base (450) to drive the ultrasonic mechanism (430) and the magnetic attraction mechanism (440) to lift along the vertical direction (Z), so that the ultrasonic transduction head (431) and the magnetic attraction mechanism (440) are in butt joint with the bottom of the kit (40).

9. The biological sample testing device of claim 1, wherein the lifting rotation module (600) comprises a movable mounting plate (610), a rotation drive mechanism (620) and a lifting drive mechanism (630);

The movable mounting plate (610) is slidably arranged in the second rack (500) along the vertical direction (Z), and the extraction and transfer device (20) is rotatably arranged on the movable mounting plate (610);

the rotary driving mechanism (620) is arranged on the movable mounting plate (610) and is in transmission connection with the extraction and transfer device (20), and the rotary driving mechanism (620) is used for driving the extraction and transfer device (20) to rotate; and

the lifting driving mechanism (630) is arranged on the second frame (500) and is in transmission connection with the movable mounting plate (610), and the lifting driving mechanism (630) is used for driving the movable mounting plate (610) to drive the extraction and transfer device (20) to lift along the vertical direction (Z).

10. The biological sample detection apparatus according to claim 9, wherein the elevation drive mechanism (630) is interposed between the rotation drive mechanism (620) and the extraction transfer device (20), wherein the extraction transfer device (20), the elevation drive mechanism (630) and the rotation drive mechanism (620) are arranged along the same straight line.

11. The biological sample detection device of claim 1, wherein the sample extraction module (700) comprises a swivel (710), a pipetting mechanism (720), and a rotational positioning mechanism (730);

The rotating seat (710) is rotatably arranged on the lifting rotating module (600);

the pipetting mechanism (720) is arranged on the rotating seat (710), and the pipetting mechanism (720) is used for sucking a sample or a reagent for transferring;

the rotary positioning mechanism (730) is disposed on the rotary seat (710) and is used for positioning the rotation angle of the rotary seat (710).

12. The biological sample detection device of claim 11, wherein the rotational positioning mechanism (730) comprises a code wheel (731), an origin wheel (732), a first position detector (733), and a second position detector (734);

the code wheel (731) and the origin wheel (732) are both arranged on the rotary seat (710), and the code wheel (731) and the origin wheel (732) are coaxial with the rotary seat (710);

the first position detector (733) and the second position detector (734) are arranged on the lifting rotation module (600) through a fixed bracket (740);

the automatic detection device comprises a plurality of code plates (731), wherein a preset number of position marks (7310) are arranged on each code plate (731), each position mark (7310) corresponds to a position when the pipetting mechanism (720) rotates at different angles, an origin mark (7320) is arranged on each origin plate (732), each origin mark (7320) corresponds to a working origin position of the rotary seat (710), a first position detector (733) is used for detecting the corresponding position mark (7310), and a second position detector (734) is used for detecting the corresponding origin mark (7320).

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