CN214668592U - Novel fluorescence detection device with oscillation - Google Patents
Novel fluorescence detection device with oscillation Download PDFInfo
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- CN214668592U CN214668592U CN202120723178.4U CN202120723178U CN214668592U CN 214668592 U CN214668592 U CN 214668592U CN 202120723178 U CN202120723178 U CN 202120723178U CN 214668592 U CN214668592 U CN 214668592U
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Abstract
The utility model particularly discloses a novel fluorescence detection device with oscillation, which comprises a fluorescence detection module, an oscillation mechanism arranged above the fluorescence detection module, a consumable material carrier arranged on the oscillation mechanism and a driving mechanism; the fluorescence detection module comprises at least one excitation light source group, at least one fluorescence condensation group and at least one light detection piece; the oscillation mechanism comprises a first supporting plate and a second supporting plate which are oppositely arranged at two sides of the fluorescence detection module, a first guide rod and a second guide rod which are respectively fixed between two end parts of the first supporting plate and the second supporting plate, a first sliding seat which is slidably sleeved on the first guide rod and the second guide rod, a third guide rod and a fourth guide rod which are vertical to the horizontal direction of the first guide rod and the second guide rod are arranged on the first sliding seat, and a second sliding seat which is slidably sleeved on the third guide rod and the fourth guide rod; the consumable carrier is arranged on the second sliding seat; the driving mechanism comprises a connecting rod, an eccentric shaft and a driving motor. The utility model discloses can be effectively with reagent vortex misce bene, improve amplification efficiency, and then guarantee the testing result accuracy.
Description
Technical Field
The utility model relates to a biological sample detection analysis field, concretely relates to novel take fluorescence detection of oscillation device.
Background
Nucleic acids (including deoxyribonucleic acid DNR and ribonucleic acid RNA) exist in various organisms, and nucleic acid detection extracts nucleic acid from various biological samples, and then detection analysis is performed through detection test paper or a detection instrument and the like. Fluorescence detection is widely used for nucleic acid detection due to its high sensitivity and convenient operation. The existing fluorescence detection device adopts a circularly heating fluorescence detection mode, so that the amplification efficiency is low, and the reagents are not uniformly mixed, thereby influencing the accuracy of a detection result.
In addition, in the system for integrated nucleic acid extraction and amplification detection of chinese patent document CN106916743A, a vibration module is disclosed, which comprises a vibration pool, buffer cotton, a vibration motor fixing ring, a vibration motor, a chip groove, a spring, a limit pin, and a buckle, and the vibration module drives the extraction cavity to vibrate, so that the lysate in the extraction cavity is mixed with the sample, the magnetic beads, and the liquid reagent, and the magnetic beads are promoted to adsorb nucleic acid. That is, vibration is set during the extraction of nucleic acid. And secondly, starting the amplification reaction by starting the heating membrane to raise the temperature to 95 ℃, and detecting the product in the amplification tube in real time by a fluorescence detection module in the amplification reaction process. Therefore, the method is also a traditional heating amplification fluorescence detection mode.
SUMMERY OF THE UTILITY MODEL
In view of the above, there is a need for a novel fluorescence detection device with oscillation that can mix reagents uniformly by eddy current, improve amplification efficiency, and ensure the accuracy of detection results.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a novel fluorescence detection device with oscillation comprises a fluorescence detection module, an oscillation mechanism arranged above the fluorescence detection module, a consumable material carrier arranged on the oscillation mechanism, and a driving mechanism;
the fluorescence detection module comprises at least one excitation light source group, at least one fluorescence light-gathering group and at least one light detection element, wherein the excitation light source group comprises an LED light source, an excitation light source light-gathering lens group and a light filter, and the fluorescence light-gathering group comprises a fluorescence light-gathering lens group and a second light filter;
the oscillation mechanism comprises a first support plate and a second support plate which are oppositely arranged at two sides of the fluorescence detection module, a first guide rod and a second guide rod which are respectively fixed between two end parts of the first support plate and the second support plate, a first sliding seat which is sleeved on the first guide rod and the second guide rod in a sliding way, a third guide rod and a fourth guide rod which are vertical to the first guide rod and the second guide rod in the horizontal direction are arranged on the first sliding seat, and a second sliding seat which is sleeved on the third guide rod and the fourth guide rod in a sliding way;
the consumable carrier is arranged on the second sliding seat;
the driving mechanism comprises a connecting rod, an eccentric shaft and a driving motor, one end of the connecting rod is fixedly connected with one side of the consumable carrier, the other end of the connecting rod is sleeved on one end of the eccentric shaft, and the other end of the eccentric shaft is connected with the driving motor.
The eccentric shaft comprises a main shaft part and an eccentric shaft part which is arranged at one end of the main shaft part and is offset relative to the axis of the main shaft part, the eccentric shaft part is sleeved at the other end of the connecting rod, the main shaft part is connected with the driving motor, and the motion track of the eccentric shaft part along the axis of the main shaft part is circular under the driving of the driving motor.
Furthermore, a speed regulating mechanism is arranged between the driving motor and the main shaft part of the eccentric shaft.
Further, the speed regulating mechanism comprises a driving large wheel sleeved at the output end of the driving motor, a driven small wheel driven by the driving large wheel through a synchronous belt, and a driven large wheel coaxially arranged with the driven small wheel; the driven bull wheel drives the main shaft part of the eccentric shaft through a synchronous belt.
Further, the driving mechanism is provided with a photoelectric sensor at a position close to the eccentric shaft.
Furthermore, a heating film, a thermistor and a temperature control switch which are electrically connected with the heating film are arranged in the consumable carrier.
The detection module driving mechanism comprises a pair of supports arranged at intervals, a fifth guide rod and a sixth guide rod which are arranged between the pair of supports in parallel, a third sliding seat which is sleeved on the fifth guide rod and the sixth guide rod in a sliding manner, and a driving device which drives the third sliding seat to slide along the fifth guide rod and the sixth guide rod; the fluorescence detection module is arranged on the third sliding seat.
Furthermore, a linear rail perpendicular to the horizontal direction of the fifth guide rod and the sixth guide rod is further arranged on the third sliding seat, and the fluorescence detection module is movably arranged on the linear rail.
Furthermore, the fluorescence detection module further comprises a support frame, at least one excitation light source hole and at least one fluorescence detection hole are adjacently arranged on the support frame, the excitation light source group is arranged below the excitation light source hole, and the fluorescence light gathering group is arranged below the fluorescence detection hole.
Further, be provided with the light trap on the consumptive material carrier.
The utility model has the advantages that: the relative slip of consumptive material carrier through oscillating mechanism's first slide and second slide can realize the all around removal in the coplanar, simultaneously under driving motor's drive, the circular motion orbit of consumptive material carrier following the eccentric shaft moves on oscillating mechanism, reagent in the consumptive material carries out rotary motion along the consumptive material inside pipe wall on the consumptive material carrier, realizes the vortex misce bene, improves amplification efficiency, and then has guaranteed the accuracy of testing result.
Drawings
FIG. 1 is a schematic structural view of a novel fluorescence detection device with oscillation according to the present invention;
FIG. 2 is an exploded view of the novel fluorescence detection device with oscillation according to the present invention;
FIG. 3 is a schematic structural diagram of an oscillating mechanism;
FIG. 4 is a schematic view of the driving mechanism;
FIG. 5 is a schematic view of the eccentric shaft structure;
FIG. 6 is a schematic view of a driving mechanism of the detection module;
FIG. 7 is a schematic view of a consumable carrier structure.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, embodiments of the present invention are combined below to describe the technical solutions of the present invention clearly and completely. It should be noted that the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
It is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", and the like, are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the device or element so indicated must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention.
The terms "first," "second," "third," "fourth," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, the definitions of "first", "second", "third", "fourth" features may explicitly or implicitly include one or more of such features.
As shown in FIG. 1, the utility model relates to a novel take fluorescence detection of oscillation device, including setting up fluorescence detection module 2 on base plate 1, locating the oscillation mechanism 3 of fluorescence detection module 2 top, arranging consumptive material carrier 4 and actuating mechanism 5 on the oscillation mechanism 3 in.
As shown in fig. 2, the oscillating mechanism 3 includes a first support plate 31 and a second support plate 32 disposed at two sides of the fluorescence detection module 2, the first support plate 31 and the second support plate 32 are vertically fixed on the base 1, a first guide rod 33 and a second guide rod 34 are fixed between two ends of an upper end of the first support plate 31 and the second support plate 32, a first slide 35 is slidably sleeved on the first guide rod 33 and the second guide rod 34, a third guide rod 36 and a fourth guide rod 37 are fixed on the first slide 35 perpendicular to a horizontal direction of the first guide rod 33 and the second guide rod 34, and a second slide 38 is slidably sleeved on the third guide rod 36 and the fourth guide rod 37.
The consumable carrier 4 is placed on the second carriage 38 and the consumables containing the nucleic acids and amplification reaction reagents are placed in the wells of the consumable carrier.
As shown in fig. 4 and 5, the driving mechanism 5 includes a connecting rod 51, an eccentric shaft 52 and a driving motor 53, the eccentric shaft 52 includes a main shaft portion 521 and an eccentric shaft portion 522 which is disposed at one end of the main shaft portion 521 and is offset with respect to the axis of the main shaft portion 521, the main shaft portion 521 of the eccentric shaft 52 is vertically and rotatably mounted on the shaft bracket through a bearing, the driving motor 53 is vertically mounted on the base 1 through a motor bracket, the output end of the driving motor 53 is connected with the lower end of the main shaft portion 521 of the eccentric shaft 52 through a timing belt, one end of the connecting rod 51 is sleeved on the eccentric shaft portion 522 at the upper end of the eccentric shaft 52, and the other end of the connecting rod 51 is fixedly connected with one side of the consumable carrier 4.
The driving motor 53 drives the eccentric shaft 52 to rotate, the eccentric shaft part 522 at the upper end of the eccentric shaft 52 rotates around the axis of the main shaft part 521, a circular motion track taking the axis of the main shaft part as a circle center is formed in an overlooking manner, the connecting rod 51 drives the consumable carrier 4 and the consumable 6 to move on the oscillating mechanism along with the motion track of the eccentric shaft part 522, and the reagent in the consumable can rotate along the inner wall of the consumable tube.
The fluorescence detection module 2 comprises at least one excitation light source group (not shown), at least one fluorescence light-gathering group (not shown) and at least one photodetection element (not shown), wherein the excitation light source group comprises an LED light source, an excitation light source light-gathering lens group and a filter, the fluorescence light-gathering group comprises a fluorescence light-gathering lens group and a second filter, and the photodetection element is a photodiode or a photomultiplier tube. Preferably, a support frame 27 of the fluorescence detection module is further provided, the support frame 27 is provided with an excitation light source hole 28 and a fluorescence detection hole 29, the excitation light source group is disposed below the excitation light source hole, and the fluorescence light-gathering group is disposed below the fluorescence detection hole.
As shown in fig. 7, the consumable carrier 4 is provided with light transmission holes 41 at both sides of the consumable.
The utility model discloses a theory of operation does: the driving motor drives the eccentric shaft to rotate, the eccentric shaft part moves along the axis circumference of the main shaft part to form a circular motion track, and the connecting rod drives the consumable carrier and the consumable to move on the oscillating mechanism along with the motion track of the eccentric shaft part, so that the reagent in the consumable rotates along the inner wall of the consumable pipe in a vortex mode, and the uniform mixing of the reagent vortex is realized; meanwhile, after the LED light source emits light, the light is condensed by the excitation light source condensing lens group, the light with the specified wavelength is obtained by the filter plate and then is irradiated on a reagent in the consumable, the reagent emits fluorescence with another wavelength to the periphery, part of the fluorescence with the other wavelength is emitted into the detection module, the fluorescence with the specified wavelength is obtained by the fluorescence condensing lens group and the second filter, the fluorescence with the specified wavelength is collected on the light detection element, and the intensity of the analysis light can finish the conventional nucleic acid amplification detection.
The utility model discloses fluorescence detection device's consumptive material carrier can realize all around the removal in the coplanar through the relative slip of oscillating mechanism's first slide and second slide, simultaneously under driving motor's drive, the circular motion orbit of consumptive material carrier following the eccentric shaft moves on oscillating mechanism, reagent in the consumptive material carrier goes up the consumptive material inside pipe wall along the consumptive material and carries out rotary motion, realize the vortex misce bene, the efficiency of augmentation is improved, and then the accuracy of testing result has been guaranteed.
As shown in fig. 6, the detection module driving mechanism includes a pair of brackets 21 fixed on the substrate 1 at intervals, a fifth guide rod 22 and a sixth guide rod 23 arranged in parallel between the pair of brackets 21, a third slide 24 slidably sleeved on the fifth guide rod and the sixth guide rod, and a driving device 25 for driving the third slide, a wire rail 26 is arranged on the third slide 24 perpendicular to the horizontal direction of the fifth guide rod and the sixth guide rod, and the fluorescence detection module 2 is movably arranged on the wire rail 26 by the driving device. Preferably, the drive means 25 is a stepper motor, and the exact position within the range of travel of the detection module can be determined. Make the removal of fluorescence detection module horizontal and vertical two directions in the horizontal plane through detection module actuating mechanism, can realize the detection to a plurality of consumptive materials on the consumptive material carrier.
In addition, the number of the excitation light source group, the number of the fluorescence light-gathering group and the number of the photodiodes are respectively two or more, so that a plurality of consumables can be detected simultaneously, the detection speed is increased, and the detection time is shortened.
A speed regulating mechanism is arranged between the driving motor and the main shaft of the eccentric shaft, and the rotation of the eccentric shaft at different speeds can be realized through the speed regulating mechanism. As shown in fig. 4, the speed adjusting mechanism includes a driving large wheel 54 sleeved at the output end of the driving motor 52, a driven small wheel 55 driven by the driving large wheel 54 through a synchronous belt, and a driven large wheel 56 coaxially arranged with the driven small wheel 55; the driven sheave 56 drives the main shaft 521 of the eccentric shaft through a timing belt. The speed regulating mechanism can also be a gear speed regulating mechanism. The driving motor can be a servo motor, a direct current brushless motor and the like, and is preferably a stepping motor, and the precise positioning of the eccentric shaft is realized by controlling the pulse of the stepping motor.
Further, a photoelectric sensor 57 is provided at a position close to the eccentric shaft 52, and a light blocking plate 58 is provided on the eccentric shaft 52, and the start point of the rotation of the eccentric shaft is determined by the light blocking plate and the photoelectric sensor.
Further, be equipped with in the consumptive material carrier 4 and add the hotting mask, with the thermistor and the temperature detect switch of hotting mask electric connection, can carry out temperature control to the reagent in consumptive material carrier, consumptive material and the consumptive material.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (9)
1. A novel fluorescence detection device with oscillation is characterized by comprising a fluorescence detection module, an oscillation mechanism arranged above the fluorescence detection module, a consumable carrier arranged on the oscillation mechanism and a driving mechanism;
the fluorescence detection module comprises at least one excitation light source group, at least one fluorescence light-gathering group and at least one light detection element, wherein the excitation light source group comprises an LED light source, an excitation light source light-gathering lens group and a light filter, and the fluorescence light-gathering group comprises a fluorescence light-gathering lens group and a second light filter;
the oscillation mechanism comprises a first support plate and a second support plate which are oppositely arranged at two sides of the fluorescence detection module, a first guide rod and a second guide rod which are respectively fixed between two end parts of the first support plate and the second support plate, a first sliding seat which is sleeved on the first guide rod and the second guide rod in a sliding way, a third guide rod and a fourth guide rod which are vertical to the first guide rod and the second guide rod in the horizontal direction are arranged on the first sliding seat, and a second sliding seat which is sleeved on the third guide rod and the fourth guide rod in a sliding way;
the consumable carrier is arranged on the second sliding seat;
the driving mechanism comprises a connecting rod, an eccentric shaft and a driving motor, one end of the connecting rod is fixedly connected with one side of the consumable carrier, the other end of the connecting rod is sleeved on one end of the eccentric shaft, and the other end of the eccentric shaft is connected with the driving motor.
2. The novel fluorescence detection device with oscillation according to claim 1, wherein the eccentric shaft comprises a main shaft portion and an eccentric shaft portion disposed at one end of the main shaft portion and offset with respect to the axis of the main shaft portion, the eccentric shaft portion is sleeved at the other end of the connecting rod, the main shaft portion is connected with the driving motor, and the movement track of the eccentric shaft portion along the axis of the main shaft portion driven by the driving motor is circular.
3. The fluorescence detecting device with oscillation as claimed in claim 2, wherein a speed adjusting mechanism is provided between the driving motor and the main shaft portion of the eccentric shaft.
4. The novel fluorescence detection device with oscillation as claimed in claim 3, wherein the speed-adjusting mechanism comprises a driving large wheel sleeved on the output end of the driving motor, a driven small wheel driven by the driving large wheel through a synchronous belt, and a driven large wheel coaxially arranged with the driven small wheel; the driven bull wheel drives the main shaft part of the eccentric shaft through a synchronous belt.
5. The novel fluorescence detection device with oscillation as claimed in any one of claims 1 to 4, wherein said driving mechanism is provided with a photoelectric sensor at a position close to said eccentric shaft.
6. The novel fluorescence detection device with oscillation as claimed in claim 1, wherein a heating film, a thermistor and a temperature control switch are arranged in the consumable carrier, and the thermistor and the temperature control switch are electrically connected with the heating film.
7. The novel fluorescence detection device with oscillation according to claim 1, further comprising a detection module driving mechanism, wherein the detection module driving mechanism comprises a pair of spaced brackets, a fifth guide rod and a sixth guide rod arranged in parallel between the pair of brackets, a third slide slidably sleeved on the fifth guide rod and the sixth guide rod, and a driving device for driving the third slide to slide along the fifth guide rod and the sixth guide rod; the fluorescence detection module is arranged on the third sliding seat.
8. The novel fluorescence detection device with oscillation according to claim 7, wherein the third slide base is further provided with a linear rail perpendicular to the horizontal direction of the fifth guide rod and the sixth guide rod, and the detection module is movably arranged on the linear rail.
9. The novel fluorescence detection device with oscillation as claimed in claim 1, wherein the consumable carrier is provided with an optically transparent hole.
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| CN202120723178.4U CN214668592U (en) | 2021-04-09 | 2021-04-09 | Novel fluorescence detection device with oscillation |
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| CN202120723178.4U CN214668592U (en) | 2021-04-09 | 2021-04-09 | Novel fluorescence detection device with oscillation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113092433A (en) * | 2021-04-09 | 2021-07-09 | 广州新成生物科技有限公司 | Fluorescent detection device with oscillation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113092433A (en) * | 2021-04-09 | 2021-07-09 | 广州新成生物科技有限公司 | Fluorescent detection device with oscillation |
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