CN219799239U

CN219799239U - Liquid phase chip detection equipment

Info

Publication number: CN219799239U
Application number: CN202222831229.2U
Authority: CN
Inventors: 王东洋
Original assignee: Shenzhen Yexin Technology Co ltd
Current assignee: Shenzhen Yexin Technology Co ltd
Priority date: 2022-10-26
Filing date: 2022-10-26
Publication date: 2023-10-03
Anticipated expiration: 2032-10-26

Abstract

The application discloses liquid phase chip detection equipment which comprises a machine base, a loading mechanism, a detection position, a pipetting mechanism and a detection device. When the liquid chip detection work is carried out, various reagents are dispatched to a reaction position through a liquid transfer mechanism to react, a solution containing particles to be analyzed is obtained, then the solution containing the particles to be analyzed is transferred to a detection position through the liquid transfer mechanism, or the reaction position is directly used as the detection position, and the detection device directly detects the particles to be analyzed in the detection position. Compared with the prior art, the device does not depend on the acquisition mechanism, saves the time for the acquisition mechanism to acquire the reacted particles to be analyzed into a special detection chamber for detection, and then outputs the particles from the detection chamber and cleans the detection chamber after detection, thereby being beneficial to improving the detection efficiency of the liquid-phase chip detection device, also being beneficial to avoiding the loss of the particles to be analyzed in the acquisition process, and being beneficial to improving the reliability of the detection result.

Description

Liquid phase chip detection equipment

Technical Field

The application relates to the technical field of biochip detection, in particular to liquid phase chip detection equipment.

Background

The liquid phase suspension chip technology is a tip molecule detection technology integrating a current collector technology, a particle synthesis technology, a molecular hybridization technology and a high-efficiency digital signal processing technology, and the principle is that known molecules (DNA, RNA, polypeptide, protein, small molecules and the like) are integrated on the surface of one or more particles to form a probe array, one or more substances to be detected in a sample are captured, one or more substances to be detected are coupled with one or more substances to be detected (fluorescent dye, fluorescent groups or fluorescent particles, raman spectrum characteristic molecules and the like) to be identified, and then the detection is carried out by an optical method. The liquid-phase suspension chip technology is used for detecting the biomolecules, has the remarkable advantages of high precision, high flux, high speed, low cost and the like, and is a novel biomolecule detection technology.

The liquid-phase suspension chip detector used in the current market mainly realizes detection based on the principles of flow cytometry and fluorescence imaging. The existing liquid-phase suspension chip detector generally comprises a liquid-transferring mechanism and a collecting mechanism, and various reagents are scheduled to react through the liquid-transferring mechanism to obtain particles to be analyzed. The reacted particles to be analyzed are collected into a special detection chamber through a collection mechanism for detection, and the particles are output from the detection chamber and cleaned after detection. This process lengthens the detection time and reduces the detection efficiency.

Disclosure of Invention

The utility model mainly solves the technical problems that: the detection efficiency of the existing liquid phase chip detection equipment is low.

In a first aspect, an embodiment provides a liquid phase chip detection apparatus, including:

the machine base is used for bearing;

the loading mechanism is arranged on the base and is used for detachably loading a reagent card, and the reagent card comprises a reagent position which is used for containing a reaction reagent;

the detection position is positioned on the machine base, the loading mechanism or the reagent card;

a pipetting mechanism for transferring a sample from a sample position for accommodating the sample to a reaction position for providing a reaction site, transferring a reagent from a reagent position to the reaction position, and/or transferring a reaction solution obtained by reacting the sample and the reagent from the reaction position to a detection position, the reaction solution containing particles to be analyzed therein, the particles being arranged in a single layer at the detection position;

And the detection device is used for receiving the optical signal emitted by the particles to be analyzed in the detection position, converting the optical signal into optical signal data, and the optical signal data are data of the analyte and/or the content of the analyte indicated by the optical signal obtained through processing.

In one embodiment, the detection device includes a detection lens, where the detection lens is used for aligning with the detection bits to receive the optical signal sent by each particle located in the detection bits, and the detection lens is set in a one-to-one correspondence with the detection bits.

In one embodiment, the detection device comprises a detection lens for aligning with a detection bit to receive an optical signal emitted by each particle located at the detection bit, at least one of the detection device and the loading mechanism has a drive assembly for driving the detection device and/or the loading mechanism in motion to align the detection lens with the detection bit, and/or to adjust the distance between the detection lens and the detection bit aligned with it.

In one embodiment, the detection device comprises a detection driving component, wherein the detection driving component is used for driving the detection lens to move along the arrangement path of the detection positions so as to enable the detection lens to move to a position opposite to the detection positions, and/or driving the detection lens to move close to and far from the aligned detection positions so as to adjust the distance between the detection lens and the aligned detection positions.

In one embodiment, the detection driving assembly comprises a first detection motor, a second detection motor, a first detection screw rod, a second detection screw rod, a first detection sliding rail, a second detection sliding rail, a first detection sliding block, a second detection sliding block and a detection connecting plate;

the first detection sliding rail is arranged on the detection connecting plate, the first detection sliding rail extends along the direction of the detection lens, which is close to and far away from the detection position, the first detection screw rod is parallel to the first detection sliding rail, the first detection sliding block is in sliding connection with the first detection sliding rail, the detection lens is connected with the first detection sliding block, the output end of the first detection motor is connected with the first detection screw rod, and the first detection motor is used for driving the first detection screw rod to rotate so as to drive the detection lens to move along the extending direction of the first detection sliding rail;

the second detects the slide rail and is on a parallel with the route of arranging of detection position, the second detects the lead screw and detects the slide rail parallel with the second, second detects slider and second and detects slide rail sliding connection, it is connected with the second to detect the connecting plate, the output of second detection motor is connected with the second and detects the lead screw, the second detects the motor and is used for driving the second and detects the lead screw rotation to drive the detection lens and detect the extending direction motion of slide rail along the second.

In one embodiment, the particle detector further comprises a light source for illuminating the particles of the detection bit such that the particles, upon illumination, emit a light signal related to the characteristics of the particles themselves.

In one embodiment, the pipetting mechanism has a sampling structure, at least one of the pipetting mechanism and the loading mechanism has a drive assembly that can drive the pipetting mechanism and/or the loading mechanism in motion to align and/or extend the sampling structure into a reagent station, a sample station, a reaction station, a detection station, a wash station, a sample dilution station, and/or a waste liquid station.

In one embodiment, the loading mechanism includes a loading plate for detachable connection with the reagent card and a loading drive assembly capable of driving the loading plate to move along a first path to move the reagent bit, the sample bit, the reaction bit, the detection bit, the wash liquid level, the wash bit, the sample dilution liquid level, the sample dilution bit, and/or the waste liquid bit to a position opposite the sampling structure.

In one embodiment, the pipetting mechanism includes a sampling needle, a pipetting drive assembly capable of driving the sampling needle along a second path to extend the sampling needle into a reagent, sample, reaction, detection, wash, sample dilution, and/or waste containment cavity opposite the sampling needle position.

In one embodiment, the pipetting mechanism further comprises a pipetting needle frame, the sampling needle is arranged on the pipetting needle frame, and the pipetting drive assembly comprises a pipetting motor, a pipetting slide rail, a pipetting screw and a pipetting slide block;

the liquid-transferring slide rail extends along a first direction, the liquid-transferring screw rod rotates to be arranged and is parallel to the liquid-transferring slide rail, the liquid-transferring slide block is movably sleeved on the liquid-transferring screw rod, the liquid-transferring slide block is in sliding connection with the liquid-transferring slide rail, the liquid-transferring slide block is connected with the liquid-transferring needle frame, the output end of the liquid-transferring motor is connected with the liquid-transferring screw rod, and the liquid-transferring motor is used for driving the liquid-transferring screw rod to rotate so as to drive the liquid-transferring slide block and the liquid-transferring needle frame to move along the first direction.

In one embodiment, the loading drive assembly comprises a loading motor, a loading slide rail, a loading screw and a loading slide block;

the loading plate is in sliding connection with the loading slide rail, the loading slide rail extends along the second direction, the loading screw rod is rotationally arranged and parallel to the loading slide rail, the loading slide block is movably sleeved on the loading screw rod, the loading slide block is connected with the loading plate, the output end of the loading motor is connected with the loading screw rod, and the loading motor is used for driving the loading screw rod to rotate so as to drive the loading slide block and the loading plate to move along the second direction.

In one embodiment, the device further comprises a sample position, a reaction position, a washing position, a sample dilution position and/or a waste liquid position, wherein the sample position, the reaction position, the washing position, the sample dilution position and/or the waste liquid position are arranged on the machine base, the reagent card and/or the loading mechanism.

In one embodiment, the loading mechanism further comprises a heating plate for providing a heated biochemical reaction environment for the reaction sites.

In an embodiment, the loading mechanism further comprises a magnetic attraction device arranged towards the reaction site, the particles comprising magnetic particles, the magnetic attraction device being arranged to generate a magnetic field to attract the magnetic particles at the side or bottom of the reaction site.

In one embodiment, the tip head position is used for bearing the tip head, the tip head position is arranged on the reagent card base and/or on the loading mechanism, the pipetting mechanism comprises a sampling needle, and the sampling needle is provided with a connecting part which is used for being detachably connected with the tip head.

In an embodiment, still include tip head separation structure, tip head separation structure includes separator and separation drive assembly, the separator activity sets up, separation drive assembly is used for driving the separator and moves between first position and second position, when the separator moves to first position, be used for promoting tip head and sampling needle's connecting portion separation, when the separator moves to the second position, do not hinder tip head and connecting portion detachable connection.

The liquid phase chip detection equipment according to the embodiment comprises a machine base, a loading mechanism, a detection position, a pipetting mechanism and a detection device. When the liquid chip detection work is carried out, various reagents are dispatched to a reaction position through a liquid transfer mechanism to react, a solution containing particles to be analyzed is obtained, then the solution containing the particles to be analyzed is transferred to a detection position through the liquid transfer mechanism, or the reaction position is directly used as the detection position, and the detection device directly detects the particles to be analyzed in the detection position. Compared with the prior art, the device does not depend on the acquisition mechanism, saves the time for the acquisition mechanism to acquire the reacted particles to be analyzed into a special detection chamber for detection, and then outputs the particles from the detection chamber and cleans the detection chamber after detection, thereby being beneficial to improving the detection efficiency of the liquid-phase chip detection device, also being beneficial to avoiding the loss of the particles to be analyzed in the acquisition process, and being beneficial to improving the reliability of the detection result.

Drawings

FIG. 1 is a schematic diagram showing a perspective view of a liquid phase chip inspection apparatus according to an embodiment of the present application;

FIG. 2 is a schematic diagram illustrating a structure of a liquid phase chip inspection apparatus according to a side view of an embodiment of the present application;

FIG. 3 is a schematic diagram illustrating a schematic structure of a liquid-phase chip testing apparatus according to an embodiment of the present application in a top view;

FIG. 4 is a schematic view showing a structure of a pipetting mechanism and tip head separation structure in accordance with an embodiment of the application;

FIG. 5 is a schematic diagram of a detecting device according to an embodiment of the present application;

FIG. 6 is a schematic view of a loading mechanism according to an embodiment of the present application;

reference numerals: 100. a base; 200. a loading mechanism; 210. a loading plate; 220. loading a drive assembly; 221. loading a motor; 222. loading a sliding rail; 223. loading a screw rod; 224. loading a sliding block; 300. a pipetting mechanism; 310. a sampling needle; 320. a pipetting drive assembly; 321. a pipetting motor; 322. a pipetting slide rail; 323. a pipetting screw; 324. a pipetting slide; 330. a pipetting needle holder; 400. a detection device; 410. detecting a lens; 420. detecting a driving assembly; 421. a first detection motor; 422. a second detection motor; 423. a first detection screw rod; 424. a second detection screw rod; 425. a first detection slide rail; 426. the second detection slide rail; 427. a first detection slider; 428. a second detection slider; 429. detecting a connecting plate; 500. a magnetic attraction device; 600. tip head separation structure; 610. a separating member; 620. separating the drive assembly; 621. separating the slide rail; 622. separating the motor; 623. separating a screw rod; 624. separating the sliding block; 700. a light source; 800. a reagent card.

Detailed Description

The utility model will be described in further detail below with reference to the drawings by means of specific embodiments. Wherein like elements in different embodiments are numbered alike in association. In the following embodiments, numerous specific details are set forth in order to provide a better understanding of the present utility model. However, one skilled in the art will readily recognize that some of the features may be omitted, or replaced by other elements, materials, or methods in different situations. In some instances, related operations of the present utility model have not been shown or described in the specification in order to avoid obscuring the core portions of the present utility model, and may be unnecessary to persons skilled in the art from a detailed description of the related operations, which may be presented in the description and general knowledge of one skilled in the art.

Furthermore, the described features, operations, or characteristics of the description may be combined in any suitable manner in various embodiments. Also, various steps or acts in the method descriptions may be interchanged or modified in a manner apparent to those of ordinary skill in the art. Thus, the various orders in the description and drawings are for clarity of description of only certain embodiments, and are not meant to be required orders unless otherwise indicated.

The numbering of the components itself, e.g. "first", "second", etc., is used herein merely to distinguish between the described objects and does not have any sequential or technical meaning. The term "coupled" as used herein includes both direct and indirect coupling (coupling), unless otherwise indicated.

The embodiment provides liquid phase chip detection equipment.

Referring to fig. 1 to 6, the liquid chip testing apparatus includes a base 100, a loading mechanism 200, a testing position, a pipetting mechanism 300 and a testing device 400.

Referring to fig. 1-6, the stand 100 is for carrying, and specifically, the stand 100 may be a base plate, a bracket, a housing, or any other support structure.

The loading mechanism 200 is disposed on the base 100, and the loading mechanism 200 is used for detachably loading the reagent card 800, and the reagent card 800 includes a reagent site for accommodating a reaction reagent. Multiple reagent cards 800 may be mounted on the loading mechanism 200 to allow for simultaneous reaction of multiple sets of samples with reagents. The reagent dose contained in the reagent card 800 may be a single-time detected dose or a multi-time detected dose, and the number of reagent sites in the reagent card 800 is at least one, and the type of reagent contained in the reagent card 800 is at least one.

In particular, the reagents stored by the reagent card 800 include particle-coated reagents and/or luminescent labeling reagents. The state of the particle-coated reagent stored in the reagent card 800 may be a dry state, a gel state, and/or a liquid state. The state of the luminescent marking agent stored within the agent card 800 may be a dry state, a gel state, and/or a liquid state.

Reagents may also include antibody reagents, buffer reagents (e.g., reagents to adjust PH or ion concentration, etc.), and the like, and the present utility model is not limited.

Some tests require the use of a diluent, and thus the reagent card 800 may also have a diluent stored therein, although the diluent may be stored in other locations of the device instead of in the reagent card 800.

Sample sites are provided on the reagent card 800 or on the loading mechanism 200, and reaction sites are provided on the reagent card 800 or on the loading mechanism 200. The reagent site, the sample site and the reaction site may be three separate accommodating cavities, or the reagent site and the reaction site may be the same accommodating cavity, or the sample site and the reaction site may be the same accommodating cavity, i.e. the reaction may be performed in the accommodating cavity of the reagent site or the sample site.

The test site is located on the housing 100, the loading mechanism 200 or the reagent card 800. In particular, when the test site is located on the reagent card 800, the test site may be a receiving cavity on the reagent card 800. When the detection site is located on the housing 100 or the loading mechanism 200, the housing 100 or the loading mechanism 200 may have a housing chamber for housing the particles to be analyzed, or the housing 100 or the loading mechanism 200 may have a structure for carrying a container, and the particles to be analyzed may be housed by the container mounted on the housing 100 or the loading mechanism 200.

The pipetting mechanism 300 is used for transferring a sample from a sample position for accommodating the sample to a reaction position for providing a reaction site, transferring a reagent from a reagent position to the reaction position, and/or transferring a reaction solution obtained by reacting the sample and the reagent from the reaction position to a detection position, wherein the reaction solution contains particles to be analyzed, and the particles are arranged in a single layer at the detection position.

Specifically, when the sample site, the reaction site, the reagent site, and the detection site are four separate accommodation chambers, the pipetting mechanism 300 needs to transfer the sample from the sample site to the reaction site, and transfer the reagent from the reagent site to the reaction site, and finally transfer the particles to be analyzed in the reaction site to the detection site. When the sample site and the reaction site are the same accommodating chamber, the pipetting mechanism 300 needs to transfer the reagent from the reagent site to the sample site. When the reagent site and the reaction site are the same accommodating chamber, the pipetting mechanism 300 needs to transfer the sample from the sample site to the reagent site. When the detection site and the reaction site are the same accommodating chamber, the pipetting mechanism 300 needs to transfer the sample from the sample site to the reaction site and transfer the reagent from the reagent site to the reaction site, and the detection device 400 directly detects the particles to be detected in the reaction site. The sample site, the reaction site, the reagent site and the detection site may be the same accommodating cavity, for example, when the reagent site and the reaction site are the same accommodating cavity and manual sample addition is adopted to the reaction site, the sample and the reagent do not need to be transferred through the pipetting mechanism 300, and the detection device 400 directly detects the particles to be detected in the reaction site.

It should be noted that although the present embodiment describes the structure of the reagent card 800, this is to help describe and understand the structure of the liquid phase chip detection apparatus, and the liquid phase chip detection apparatus may not include the reagent card 800. That is, in one embodiment, the liquid phase chip detection apparatus includes a reagent card 800, the reagent card 800 being an integral part of the liquid phase chip detection apparatus. In another embodiment, the liquid phase chip detection apparatus does not include the reagent card 800, and the reagent card 800 is a use object of the liquid phase chip detection apparatus, not an integral part of the liquid phase chip detection apparatus. It is understood that when the liquid chip detection apparatus does not include the reagent card 800 and the detection site is located on the reagent card 800, the liquid chip detection apparatus may not include the detection site.

Based on this, the liquid phase chip detection apparatus may include a sample site, a reaction site, a washing site, a sample dilution site, and/or a waste liquid site, which are provided on the base 100, the reagent card 800, and/or the loading mechanism 200.

Referring to fig. 5, in one embodiment, the liquid chip detection apparatus further includes a light source 700, where the light source 700 is used to irradiate the particles in the detection position, so that the particles emit light signals related to the characteristics of the particles after being irradiated. Specifically, the particles and/or luminescent substances near the particles can be provided, and the luminescent substances can emit characteristic spectra under the excitation of light. The luminescent substance can be fluorescent dye, fluorescent gene, fluorescent particles, quantum dots, time-resolved luminescent material, photo-excited chemiluminescent material, raman spectrum characteristic molecule, etc., and the utility model is not limited.

In other embodiments, the liquid phase chip detection device may also not include the light source 700, but may employ methods that do not require the light source 700 in chemiluminescence and/or electrochemiluminescence.

In one embodiment, the particles to be analyzed are contacted with a substrate comprising a chemiluminescent reaction-related reagent to cause the particles and/or the substrate contacted with the particles to undergo a chemiluminescent reaction to emit a light signal related to the properties of the particles themselves.

In another embodiment, the liquid chip detection device further comprises an electrode, wherein the electrode is used for applying an electric field to the particles to be analyzed at the detection position, so that the particles to be analyzed and/or the buffer solution contacted with the particles generate electrochemiluminescence reaction, and light signals related to the characteristics of the particles to be analyzed are emitted. In some embodiments of methods using electrochemiluminescence, the detection site may also be used to house a buffer solution that is used to effect and/or promote the electrochemiluminescence reaction.

The detection device 400 is configured to receive an optical signal emitted by a particle to be analyzed in a detection position, and convert the optical signal into optical signal data, where the optical signal data is processed to obtain an analyte and/or an analyte content indicated by the optical signal.

Referring to fig. 4-6, in one embodiment, the inspection apparatus 400 includes an inspection lens 410, the inspection lens 410 is configured to align with the inspection bits to receive the optical signal emitted by each particle located at the inspection bits, at least one of the inspection apparatus 400 and the loading mechanism 200 has a driving component configured to drive the inspection apparatus 400 and/or the loading mechanism 200 to move so as to align the inspection lens 410 with the inspection bits, and/or adjust the distance between the inspection lens 410 and the aligned inspection bits.

When detecting particles to be detected in the detection position, at least one of the detection device 400 and the loading mechanism 200 is driven to move by the driving component, so that the detection lens 410 is aligned with the detection position, and the distance between the detection lens 410 and the detection position is adjusted to be a proper detection distance.

Specifically, the driving component may drive the detection device 400 to move independently, or the driving component may drive the loading mechanism 200 to move independently, or the driving component may drive the detection device 400 and the loading mechanism 200 to move together, so that the detection lens 410 is aligned with the detection position, and the distance between the detection lens 410 and the detection position is adjusted to a suitable detection distance.

Referring to fig. 4-6, in one embodiment, the inspection apparatus 400 includes an inspection driving component 420, where the inspection driving component 420 is configured to drive the inspection lens 410 to move along an arrangement path of inspection bits to move the inspection lens 410 to a position opposite to the inspection bits, and/or to drive the inspection lens 410 to move closer to and farther from the inspection bits aligned therewith to adjust a distance between the inspection lens 410 and the inspection bits aligned therewith.

When the particles to be detected in one detection position need to be detected, the detection driving assembly 420 drives the lens to move along the arrangement path of the detection position, so that the detection lens 410 moves to a position opposite to the detection position, and drives the detection lens 410 to move close to and far from the aligned detection position, so that the distance between the detection lens 410 and the detection position is adjusted to a proper detection distance, and the accuracy of detection work is ensured.

Alternatively, in other embodiments, when the inspection lens 410 has been configured to align with the inspection bit, for example, the inspection lens 410 is aligned with the inspection bit in a one-to-one correspondence, the inspection drive assembly 420 may simply drive the inspection lens 410 toward and away from the inspection bit in which it is aligned to adjust the distance between the inspection lens 410 and the inspection bit to the appropriate inspection distance. Similarly, when the distance between the detection lens 410 and the detection bit has been configured to be a suitable detection distance, the detection driving component 420 may simply drive the detection lens 410 to align with the detection bit.

Referring to fig. 4-6, in one embodiment, the detection drive assembly 420 includes a first detection motor 421, a second detection motor 422, a first detection screw 423, a second detection screw 424, a first detection slide 425, a second detection slide 426, a first detection slide 427, a second detection slide 428, and a detection connection plate 429.

The first detection slide rail 425 is arranged on the detection connecting plate 429, the first detection slide rail 425 extends along the direction that the detection lens 410 is close to and far away from the detection position, the first detection screw rod 423 is parallel to the first detection slide rail 425, the first detection slide block 427 is in sliding connection with the first detection slide rail 425, the detection lens 410 is connected with the first detection slide block 427, the output end of the first detection motor 421 is connected with the first detection screw rod 423, and the first detection motor 421 is used for driving the first detection screw rod 423 to rotate so as to drive the detection lens 410 to move along the extending direction of the first detection slide rail 425.

The second detection slide rail 426 is parallel to the arrangement path of the detection position, the second detection screw rod 424 is parallel to the second detection slide rail 426, the second detection slide block 428 is in sliding connection with the second detection slide rail 426, the detection connecting plate 429 is connected with the second detection slide block 428, the output end of the second detection motor 422 is connected with the second detection screw rod 424, and the second detection motor 422 is used for driving the second detection screw rod 424 to rotate so as to drive the detection lens 410 to move along the extending direction of the second detection slide rail 426.

When the particles to be detected in one detection position need to be detected, the second detection motor 422 can drive the second detection screw 424 to rotate, so as to drive the detection lens 410 to move along the extending direction of the second detection slide rail 426, so that the detection lens 410 moves to a position aligned with the detection position. And the first detection motor 421 drives the first detection screw 423 to rotate, so as to drive the detection lens 410 to move along the extending direction of the first detection sliding rail 425, so as to adjust the distance between the detection lens 410 and the detection position to a proper detection distance. Specifically, in the present embodiment, the second detection slide rail 426 extends along the first direction, and the first detection slide rail 425 extends along the third direction. In other embodiments, the extending directions of the first detecting sliding rail 425 and the second detecting sliding rail 426 may be flexibly set according to actual requirements.

Of course, in other embodiments, the detection driving assembly 420 may alternatively use a driving cylinder, a belt structure driven by a motor, or other suitable linear driving module to drive the lens of the detection member to move.

Referring to fig. 4-6, in one embodiment, the detecting device 400 includes a detecting lens 410, the detecting lens 410 is used for aligning with the detecting bits to receive the optical signal emitted by each particle located in the detecting bits, and the detecting lens 410 is disposed in a one-to-one correspondence with the detecting bits.

Since the detection lenses 410 are disposed in one-to-one correspondence with the detection bits, the operations of aligning and adjusting the distance between the detection lenses 410 and the detection bits can be reduced, thereby facilitating the improvement of the detection efficiency.

Referring to fig. 4-6, in one embodiment, pipetting mechanism 300 has a sampling configuration and at least one of pipetting mechanism 300 and loading mechanism 200 has a drive assembly capable of driving movement of pipetting mechanism 300 and/or loading mechanism 200 to align and/or extend the sampling configuration into a reagent station, sample station, reaction station, detection station, wash station, sample dilution station, and/or waste station.

Specifically, in one embodiment, the pipetting mechanism 300 may be driven in motion only by the drive assembly to enable the sampling structure to align and/or extend into the reagent, sample, reaction, detection, wash, sample dilution, and/or waste sites.

In another embodiment, the loading mechanism 200 may be driven solely by the drive assembly to enable the sampling structure to be aligned with and/or extend into the reagent, sample, reaction, test, wash, sample dilution, and/or waste sites.

In another embodiment, the pipetting mechanism 300 and the loading mechanism 200 may also be driven in a common motion by a drive assembly to enable the sampling structure to be aligned and/or extended into a reagent site, a sample site, a reaction site, a detection site, a wash level, a wash site, a sample dilution level, a sample dilution site, and/or a waste site.

Alternatively, the sampling structure may be directly aligned with and extend into the receiving cavity corresponding to the reagent site, sample site, reaction site, detection site, wash level, wash site, sample dilution level, sample dilution site, and/or waste level by the sampling needle 310 itself to aspirate or output the liquid. The sampling structure can also select a structure capable of installing the tip, at the moment, the sampling structure does not need to stretch into a containing cavity for containing corresponding liquid, and only the tip installed on the sampling structure stretches into the containing cavity.

Referring to fig. 4-6, in one embodiment, the pipetting mechanism 300 further includes a pipette channel having one end in communication with the sampling needle 310 and the other end connected to a fluid drive. The fluid drive may drive the liquid through the sampling needle 310 into the pipette channel for temporary storage or further transfer. Of course, in other embodiments, the pipetting mechanism 300 may not include a pipette channel when the sampling needle 310 itself has satisfied the need for temporary storage and further transfer.

Referring to fig. 4-6, in one embodiment, the loading mechanism 200 includes a loading plate 210 and a loading drive assembly 220, the loading plate 210 is configured to be removably coupled to the reagent card 800, and the loading drive assembly 220 is capable of driving the loading plate 210 along a first path to move a reagent site, a sample site, and/or a reaction site to a position opposite the sampling needle 310.

When it is desired to transfer the liquid from the reagent level, sample level, reaction level, detection level, wash level, sample dilution level, and/or waste level, the loading mechanism 200 is driven along the first path by the loading drive assembly 220 such that the sampling structure is aligned with the holding cavity of the reagent level, sample level, reaction level, detection level, wash level, sample dilution level, and/or waste level.

Specifically, in one embodiment, referring to fig. 1-6, a "first path" refers to a path of movement of the loading plate 210 in a horizontal direction, and a "second path" refers to a path of movement of the sampling needle 310 in a vertical direction. In other embodiments, the "first path" and the "second path" may be a movement path in which a horizontal direction, a vertical direction, or a plurality of directions are sequentially combined, and the "first path" is not limited to a straight path, but may be a circular, circular arc, or other suitable shape.

The power source of the loading drive assembly 220 may be a motor, a cylinder, a hydraulic pump, and/or other suitable driving components, and the transmission structure of the loading drive assembly 220 may be a screw slider, a chain transmission structure, a belt transmission structure, a gear transmission structure, and/or other suitable transmission structure.

Referring to fig. 4-6, in one embodiment, pipetting mechanism 300 comprises a sampling needle 310, a pipetting drive assembly 320 and a fluid drive, where pipetting drive assembly 320 is capable of driving sampling needle 310 along a second path to extend sampling needle 310 into a reagent site, a sample site, a reaction site, a detection site, a wash site, a sample dilution site and/or a waste site containing cavity opposite sampling needle 310.

When it is desired to transfer the liquid of the reagent site, sample site, reaction site, detection site, wash site, sample dilution site, and/or waste site, the sampling needle 310 is driven by the pipetting drive assembly 320 along a second path such that the sampling needle 310 protrudes into the holding cavity of the reagent site, sample site, reaction site, detection site, wash site, sample dilution site, and/or waste site opposite the sampling needle 310 for aspirating and/or evacuating the liquid through the sampling needle 310.

Referring to fig. 4-6, in one embodiment, the pipetting mechanism 300 further includes a pipetting needle holder 330, the sampling needle 310 is disposed on the pipetting needle holder 330, and the pipetting drive assembly 320 includes a pipetting motor 321, a pipetting slide 322, a pipetting screw 323, and a pipetting slide 324.

The pipetting slide rail 322 extends along the first direction, the pipetting screw 323 rotates and sets up, and is parallel with pipetting slide rail 322, and pipetting slide 324 movable sleeve is established on pipetting screw 323, pipetting slide 324 and pipetting slide rail 322 sliding connection, pipetting slide 324 are connected with pipetting needle frame 330, and pipetting motor 321's output is connected with pipetting screw 323, and pipetting motor 321 is used for driving pipetting screw 323 to rotate to drive pipetting slide 324 and pipetting needle frame 330 along the first direction motion.

When the sampling needle 310 needs to be driven to move, the pipetting screw 323 is driven to rotate by the pipetting motor 321, so that the pipetting slider 324 and the pipetting needle holder 330 are driven to move along the first direction, and further the sampling needle 310 is driven.

Referring to fig. 4-6, in one embodiment, the load drive assembly 220 includes a load motor 221, a load slide rail 222, a load screw 223, and a load slide 224.

The loading plate 210 is slidably connected with the loading slide rail 222, the loading slide rail 222 extends along the second direction, the loading screw rod 223 is rotatably arranged and parallel to the loading slide rail 222, the loading slide block 224 is movably sleeved on the loading screw rod 223, the loading slide block 224 is connected with the loading plate 210, the output end of the loading motor 221 is connected with the loading screw rod 223, and the loading motor 221 is used for driving the loading screw rod 223 to rotate so as to drive the loading slide block 224 and the loading plate 210 to move along the second direction.

When the loading plate 210 needs to be driven to move, the loading screw 223 is driven to rotate by the loading motor 221, so that the loading slide block 224 and the loading plate 210 are driven to move along the second direction.

It should be noted that, referring to fig. 1, in one embodiment, the "first direction" refers to a vertical direction, the "second direction" and the "third direction" refer to two directions perpendicular to each other in a horizontal plane, that is, the "first direction" refers to a direction indicated by an arrow a, the "second direction" refers to a direction indicated by an arrow b, and the "third direction" refers to a direction indicated by an arrow c. In other embodiments, "first direction", "second direction" and "third direction" may be defined as other directions (e.g., the "first direction" may not be a vertical direction but an oblique direction at an acute angle to the horizontal plane), as long as the "first direction", "second direction" and "third direction" are perpendicular to each other.

Referring to FIG. 6, in one embodiment, the loading mechanism 200 further includes a heating plate for providing a heating environment for the reaction sites. Specifically, a heating plate may be disposed at the bottom of the loading plate 210, and the reaction solution is incubated by providing a heated biochemical reaction environment for the reaction of the reagent and the sample through the heating plate.

Referring to fig. 6, in one embodiment, the loading mechanism 200 further includes a magnetic attraction device 500, the magnetic attraction device 500 is disposed towards the reaction site, the particles include magnetic particles, and the magnetic attraction device 500 is used for generating a magnetic field to attract the magnetic particles at the side or bottom of the reaction site. Specifically, the magnetic attraction device 500 may be disposed at a side of the reaction site.

Referring to fig. 4-6, in one embodiment, the liquid chip detection apparatus further includes a tip head position for carrying a tip head, where the tip head position is disposed on the base 100, the reagent card 800, and/or the loading mechanism 200, and the pipetting mechanism 300 includes a sampling needle 310, where the sampling needle 310 has a connection portion for detachably connecting with the tip head.

The tip head is carried by the tip head position, the sampling needle 310 can be detachably connected with the tip head through the connecting part, so that the tip head is extracted through the movement of the sampling needle 310, and corresponding liquids such as reagents, samples or cleaning liquid are extracted through the extension of the tip head into the corresponding accommodating cavity.

Specifically, when the tip head is disposed on the reagent card 800, the tip head may be disposed on the tip head of the reagent card 800, when the tip head is required to be used by the liquid chip detection device, the tip head is extracted from the tip head, after the detection is completed, the waste tip head is replaced by the liquid chip detection device, and when the used reagent card 800 is discarded by a worker, the waste tip head is discarded. On the one hand, the garbage collection container used for containing the waste tip head is not required to be arranged in the biological detection equipment, the occupied space of the garbage collection container is reduced, the size of the biological detection equipment is reduced, on the other hand, the operation of discarding the waste tip head in the garbage collection container is not required to be carried out by staff, and the operation of the staff is simplified.

Referring to fig. 4-6, in an embodiment, the liquid-phase chip detection apparatus further includes a tip head separation structure 600, where the tip head separation structure 600 includes a separation member 610 and a separation driving assembly 620, the separation member 610 is movably disposed, the separation driving assembly 620 is configured to drive the separation member 610 to move between a first position and a second position, and when the separation member 610 moves to the first position, the separation member 610 is configured to push the tip head to separate from the connection portion of the sampling needle 310, and when the separation member 610 moves to the second position, the tip head is not blocked from being detachably connected to the connection portion.

When the used tip head needs to be separated from the connecting portion of the sampling needle 310, the separation member 610 is driven by the separation driving assembly 620 to move to the first position, the separation member 610 pushes the tip head to be separated from the connecting portion of the sampling needle 310, and then the separation member 610 is driven by the separation driving assembly 620 to move to the second position, so that the separation member 610 does not obstruct the detachable connection of the connecting portion and the new tip head.

Specifically, the power source of the separation driving assembly 620 may be a motor, a cylinder, a hydraulic pump, and/or other suitable driving components, and the transmission structure of the separation driving assembly 620 may be a screw slider, a chain transmission structure, a belt transmission structure, a gear transmission structure, and/or other suitable transmission structure.

Referring to fig. 4-6, in one embodiment, the separation driving assembly 620 includes a separation slide rail 621, a separation motor 622, a separation screw 623, and a separation slide block 624, wherein the separation slide rail 621 extends along a first direction, the separation screw 623 is rotatably disposed and parallel to the separation slide rail 621, the separation slide block 624 is movably sleeved on the separation screw 623, the separation member 610 is connected with the separation slide block 624, an output end of the separation motor 622 is connected with the separation screw 623, and the separation motor 622 is used for driving the separation screw 623 to rotate, thereby driving the separation slide block 624 and the separation member 610 to move along the first direction. The separating member 610 has a separating through hole, the sampling needle 310 passes through the separating through hole, the tip cap is sleeved on the connecting portion of the sampling needle 310 and is clamped with the connecting portion, and the diameter of the separating through hole is smaller than the maximum diameter of the outer wall of the tip cap, so that the tip cap can be pushed to be separated from the connecting portion of the sampling needle 310 when the separating member 610 moves to the first position.

The foregoing description of the utility model has been presented for purposes of illustration and description, and is not intended to be limiting. Several simple deductions, modifications or substitutions may also be made by a person skilled in the art to which the utility model pertains, based on the idea of the utility model.

Claims

1. A liquid phase chip detection apparatus, comprising:

the machine base plays a bearing role;

2. The liquid-phase chip inspection apparatus according to claim 1, wherein the inspection device includes an inspection lens for aligning with the inspection bits to receive the optical signal emitted from each particle located at the inspection bits, and the inspection lens is disposed in one-to-one correspondence with the inspection bits.

3. The liquid phase chip inspection apparatus according to claim 1, wherein the inspection device includes an inspection lens for aligning with the inspection site to receive the optical signal emitted from each particle located at the inspection site, and at least one of the inspection device and the loading mechanism has a driving assembly for driving the inspection device and/or the loading mechanism to move so as to align the inspection lens with the inspection site, and/or adjusting the distance between the inspection lens and the inspection site aligned with the inspection lens.

4. A liquid phase chip inspection apparatus according to claim 3, wherein the inspection device includes an inspection drive assembly for driving the inspection lens along the inspection position alignment path to move the inspection lens to a position opposite the inspection position and/or to move the inspection lens toward and away from the inspection position in which it is aligned to adjust the distance between the inspection lens and the inspection position in which it is aligned.

5. The liquid phase chip inspection apparatus according to claim 4, wherein the inspection driving assembly comprises a first inspection motor, a second inspection motor, a first inspection screw, a second inspection screw, a first inspection slide rail, a second inspection slide rail, a first inspection slide block, a second inspection slide block, and an inspection connection plate;

6. The liquid phase chip test apparatus according to claim 1, further comprising a light source for irradiating the particles of the test site such that the particles emit light signals related to the characteristics of the particles themselves after being irradiated.

7. The liquid phase chip testing apparatus of claim 1, wherein the pipetting mechanism has a sampling structure, and at least one of the pipetting mechanism and the loading mechanism has a drive assembly capable of driving the pipetting mechanism and/or the loading mechanism to move so that the sampling structure can be aligned with and/or extended into a reagent station, a sample station, a reaction station, a test station, a wash level, a wash station, a sample dilution level, a sample dilution station, and/or a waste liquid station.

8. The liquid phase chip testing apparatus according to claim 7, wherein the loading mechanism includes a loading plate for detachable connection with the reagent card and a loading drive assembly capable of driving the loading plate along a first path to move the reagent level, the sample level, the reaction level, the test level, the wash level, the sample dilution level, and/or the waste liquid level to a position opposite the sampling structure.

9. The liquid phase chip testing apparatus of claim 8, wherein the pipetting mechanism includes a sampling needle, a pipetting drive assembly and a fluid drive, the pipetting drive assembly being capable of driving the sampling needle along a second path to extend the sampling needle into a reagent site, a sample site, a reaction site, a test site, a wash site, a sample dilution site and/or a waste site containing cavity opposite the sampling needle site.

10. The liquid phase chip detection apparatus of claim 9, wherein the pipetting mechanism further comprises a pipetting needle holder on which the sampling needle is disposed, the pipetting drive assembly comprising a pipetting motor, a pipetting slide, a pipetting screw, and a pipetting slide;

11. The liquid phase chip inspection apparatus according to claim 9, wherein the loading driving assembly comprises a loading motor, a loading slide rail, a loading screw, and a loading slider;

12. The liquid phase chip detection apparatus according to any one of claims 1 to 11, further comprising a sample site, a reaction site, a wash site, a sample dilution site, and/or a waste liquid site, wherein the sample site, the reaction site, the wash site, the sample dilution site, and/or the waste liquid site are provided on the housing, the reagent card, and/or the loading mechanism.

13. The liquid phase chip testing apparatus according to any one of claims 1 to 11, wherein the loading mechanism further comprises a heating plate for providing a heated biochemical reaction environment for the reaction sites.

14. The liquid phase chip detection apparatus according to any one of claims 1 to 11, wherein the loading mechanism further comprises a magnetic attraction device disposed toward the reaction site, the particles including magnetic particles, the magnetic attraction device being configured to generate a magnetic field to attract the magnetic particles to a side or bottom of the reaction site.

15. The liquid phase chip testing apparatus according to any one of claims 1-11, further comprising a tip head position for carrying a tip head, the tip head position being provided on the housing, the reagent card and/or on the loading mechanism, the pipetting mechanism comprising a sampling needle having a connection for detachable connection with the tip head.

16. The liquid phase chip testing apparatus of claim 15, further comprising a tip head separation structure, the tip head separation structure comprising a separation member and a separation driving assembly, the separation member being movably disposed, the separation driving assembly being configured to drive the separation member between a first position and a second position, the separation member being configured to push the tip head away from the connection portion of the sampling needle when moved to the first position, the separation member being configured to not hinder the tip head from being detachably connected to the connection portion when moved to the second position.