CN113092433B - Fluorescent detection device with oscillation - Google Patents
Fluorescent detection device with oscillation Download PDFInfo
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- CN113092433B CN113092433B CN202110382895.XA CN202110382895A CN113092433B CN 113092433 B CN113092433 B CN 113092433B CN 202110382895 A CN202110382895 A CN 202110382895A CN 113092433 B CN113092433 B CN 113092433B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- General Physics & Mathematics (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention specifically discloses a fluorescence detection device with oscillation, which comprises a substrate, a fluorescence detection module arranged on the substrate, an oscillation mechanism arranged above the fluorescence detection module, a consumable carrier arranged on the oscillation mechanism and a driving mechanism; the fluorescence detection module comprises at least one excitation light source group, at least one fluorescence condensing group and at least one light detection piece; the oscillating mechanism comprises a first supporting plate, a second supporting plate, a first guide rod and a second guide rod, wherein the first supporting plate and the second supporting plate are oppositely arranged at two sides of the fluorescence detection module, the first guide rod and the second guide rod are respectively fixed between two end parts of the first supporting plate and the second supporting plate, a first sliding seat is slidably sleeved on the first guide rod and the second guide rod, a third guide rod and a fourth guide rod which are perpendicular to the horizontal directions of the first guide rod and the second guide rod are arranged on the first sliding seat, and a second sliding seat is slidably sleeved on the third guide rod and the fourth guide rod; the consumable carrier is arranged on the second sliding seat; the driving mechanism comprises a connecting rod, an eccentric shaft and a driving motor. The invention can effectively mix the reagent vortex uniformly, improve the amplification efficiency and further ensure the accuracy of the detection result.
Description
Technical Field
The invention relates to the field of biological sample detection and analysis, in particular to a fluorescent detection device with oscillation.
Background
Nucleic acids (including deoxyribonucleic acid (DNR) and ribonucleic acid (RNA)) are present in various organisms, and nucleic acid detection analysis is performed by extracting nucleic acids from various biological samples and then performing detection analysis by test strips, detection instruments, or the like. And fluorescence detection is widely applied to nucleic acid detection due to high sensitivity and convenient operation. The existing fluorescence detection device adopts a fluorescence detection mode of cyclic heating, has low amplification efficiency and uneven reagent mixing, and further influences the accuracy of detection results.
In addition, in the integrated nucleic acid extraction and amplification detection system of the Chinese patent document CN106916743A, a vibration module is disclosed, wherein the vibration module comprises a vibration pool, buffer cotton, a vibration motor fixing ring, a vibration motor, a chip groove, a spring, a limiting pin and a buckle, and the vibration module drives an extraction cavity to vibrate, so that a lysate in the extraction cavity is mixed with a sample, magnetic beads and a liquid reagent, and the magnetic beads are promoted to adsorb nucleic acid. That is, vibration is set during the extraction of nucleic acid. Secondly, starting the amplification reaction by starting the heating film to raise the temperature to 95 ℃, and detecting the products in the amplification tube in real time through the fluorescent detection module in the amplification reaction process. It is still a traditional way of thermal amplification fluorescence detection.
Disclosure of Invention
In view of the above, it is necessary to provide a fluorescence detection device with oscillation that can effectively mix the reagent vortex uniformly, improve the amplification efficiency, and further ensure the accuracy of the detection result.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a fluorescence detection device with oscillation comprises a substrate, a fluorescence detection module arranged on the substrate, an oscillation mechanism arranged above the fluorescence detection module, a consumable carrier arranged on the oscillation mechanism and a driving mechanism;
the fluorescence detection module comprises at least one excitation light source group, at least one fluorescence condensing group and at least one light detection piece, wherein the excitation light source group comprises an LED light source, an excitation light source condensing lens group and a light filter, and the fluorescence condensing group comprises a fluorescence condensing lens group and a second light filter;
the oscillating mechanism comprises a first supporting plate, a second supporting plate, a first guide rod, a second guide rod, a first sliding seat and a second sliding seat, wherein the first supporting plate and the second supporting plate are oppositely arranged at two sides of the fluorescence detection module, the first guide rod and the second guide rod are respectively fixed between two end parts of the first supporting plate and the second supporting plate, the first sliding seat is slidably sleeved on the first guide rod and the second guide rod, the first sliding seat is provided with a third guide rod and a fourth guide rod which are perpendicular to the horizontal direction of the first guide rod and the second guide rod, and the second sliding seat is slidably sleeved on the third guide rod and the fourth guide rod;
the consumable carrier is arranged on the second sliding seat;
the driving mechanism comprises a connecting rod, an eccentric shaft and a driving motor, one end of the connecting rod is fixedly connected with one side of the consumable carrier, the other end of the connecting rod is sleeved at one end of the eccentric shaft, and the other end of the eccentric shaft is connected with the driving motor.
Further, the eccentric shaft comprises a main shaft part and an eccentric shaft part which is arranged at one end of the main shaft part and is offset relative to the axis of the main shaft part, the eccentric shaft part is sleeved at the other end of the connecting rod, the main shaft part is connected with the driving motor, and the movement track of the eccentric shaft part along the axis of the main shaft part is circular under the driving of the driving motor.
Further, a speed regulating mechanism is arranged between the driving motor and the main shaft part of the eccentric shaft.
Further, the speed regulating mechanism comprises a driving large wheel sleeved at the output end of the driving motor, a driven small wheel driven by the driving large wheel through a synchronous belt, and a driven large wheel coaxially arranged with the driven small wheel; the driven large wheel drives the main shaft part of the eccentric shaft through a synchronous belt.
Further, the driving mechanism is provided with a photoelectric sensor at a position close to the eccentric shaft.
Further, a heating film, a thermistor electrically connected with the heating film and a temperature control switch are arranged in the consumable carrier.
Further, the device also comprises a detection module driving mechanism, wherein the detection module driving mechanism comprises a pair of brackets fixed on the base plate at intervals, a fifth guide rod and a sixth guide rod which are arranged between the brackets in parallel, a third sliding seat which is sleeved on the fifth guide rod and the sixth guide rod in a sliding way, and a third driving mechanism for driving the third sliding seat
The driving device slides the sliding seat along the fifth guide rod and the sixth guide rod; the fluorescence detection module is arranged on the third sliding seat.
Further, the third sliding seat is further provided with a line rail perpendicular to the horizontal directions of the fifth guide rod and the sixth guide rod, and the fluorescence detection module is movably arranged on the line rail.
Further, the fluorescence detection module further comprises a support frame, at least one excitation light source hole and at least one fluorescence detection hole are adjacently arranged on the support frame, the excitation light source group is arranged below the excitation light source hole, and the fluorescence light gathering group is arranged below the fluorescence detection hole.
Further, the consumable carrier is provided with a light hole.
The beneficial effects of the invention are as follows: the consumable carrier can move around in the coplanar through the relative slip of the first slide and the second slide of oscillating mechanism, simultaneously under driving motor's drive, the consumable carrier moves on oscillating mechanism along the circular motion orbit of eccentric shaft, and reagent in the consumable carries out rotary motion along the consumable pipe inner wall on the consumable carrier, realizes vortex mixing, improves amplification efficiency, and then has guaranteed the accuracy of testing result.
Drawings
FIG. 1 is a schematic diagram of a fluorescence detection device with oscillation according to the present invention;
FIG. 2 is a schematic diagram showing a fluorescent detection device with oscillation in a disassembled state;
FIG. 3 is a schematic diagram of an oscillating mechanism;
FIG. 4 is a schematic diagram of a driving mechanism;
FIG. 5 is a schematic diagram of the eccentric shaft structure;
FIG. 6 is a schematic diagram of a detection module driving mechanism;
fig. 7 is a schematic view of a consumable carrier structure.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be further clearly and completely described in the following in conjunction with the embodiments of the present invention. It should be noted that the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It is to be understood that the terms "upper," "lower," "front," "rear," "left," "right," and the like indicate an orientation or positional relationship based on that shown in the drawings, and are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or element in question must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.
The terms "first," "second," "third," "fourth," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a definition of "a first", "a second", "a third" or a fourth "feature may explicitly or implicitly include one or more of such features.
As shown in fig. 1, the fluorescence detection device with oscillation of the present invention includes a substrate 1, a fluorescence detection module 2 disposed on the substrate 1, an oscillation mechanism 3 disposed above the fluorescence detection module 2, a consumable carrier 4 disposed on the oscillation mechanism 3, and a driving mechanism 5.
As shown in fig. 2, the oscillating mechanism 3 includes a first support plate 31 and a second support plate 32 which are oppositely disposed at two sides of the fluorescent detection module 2, the first support plate 31 and the second support plate 32 are respectively vertically fixed on the base 1, a first guide rod 33 and a second guide rod 34 are respectively fixed between two ends of upper ends of the first support plate 31 and the second support plate 32, a first slide 35 is slidably sleeved on the first guide rod 33 and the second guide rod 34, a third guide rod 36 and a fourth guide rod 37 are vertically fixed on the first slide 35 in a horizontal direction with the first guide rod 33 and the second guide rod 34, and a second slide 38 is slidably sleeved on the third guide rod 36 and the fourth guide rod 37.
The consumable carrier 4 is placed on the second slide 38 and consumables containing nucleic acids and amplification reaction reagents are placed in the wells of the consumable carrier.
As shown in fig. 4 and 5, the driving mechanism 5 includes a connecting rod 51, an eccentric shaft 52 and a driving motor 53, the eccentric shaft 52 includes a main shaft 521 and an eccentric shaft 522 provided at one end of the main shaft 521 and offset with respect to the axis of the main shaft 521, the main shaft 521 of the eccentric shaft 52 is vertically rotatably mounted on a shaft bracket through a bearing, the driving motor 53 is vertically mounted on the base 1 through a motor bracket, an output end of the driving motor 53 is connected with a lower end of the main shaft 521 of the eccentric shaft 52 through a timing belt, one end of the connecting rod 51 is sleeved on the eccentric shaft 522 at an upper end of the eccentric shaft 52, and the other end of the connecting rod 51 is fixedly connected with one side of the consumable carrier 4.
The driving motor 53 drives the eccentric shaft 52 to rotate, the eccentric shaft 522 at the upper end of the eccentric shaft 52 moves circularly around the axis of the main shaft 521, a circular movement track taking the axis of the main shaft as the center of a circle is formed in overlooking mode, the connecting rod 51 drives the consumable carrier 4 and the consumable 6 to move on the oscillating mechanism along the movement track of the eccentric shaft 522, and the reagent in the consumable can perform eddy current rotation along the inner wall of the consumable pipe.
As shown in fig. 6, the fluorescence detection module 2 includes at least one excitation light source group (not shown), at least one fluorescence condensing group (not shown), at least one light detecting member (not shown) and a support frame 27, the support frame 27 is provided with an excitation light source hole 28 and a fluorescence detecting hole 29, the excitation light source group is disposed below the excitation light source hole, the excitation light source group includes an LED light source, an excitation light source condensing lens group and an optical filter, the fluorescence condensing group is disposed below the fluorescence detecting hole, the fluorescence condensing group includes a fluorescence condensing lens group and a second optical filter, and the light detecting member is a photodiode or a photomultiplier.
As shown in fig. 7, both sides of the consumable carrier 4 are provided with light holes 41.
The working principle of the invention is as follows: the driving motor drives the eccentric shaft to rotate, the eccentric shaft moves along the axis of the main shaft part in a circular motion track, the connecting rod drives the consumable carrier and the consumable to move on the oscillating mechanism along the motion track of the eccentric shaft, so that reagents in the consumable rotate in a vortex manner along the inner wall of the consumable pipe, and uniform mixing of reagent vortex is realized; meanwhile, after the LED light source emits light, the light is condensed by the excitation light source condensing lens group, excitation light with a specified wavelength is obtained by the optical filter, the excitation light passes through the excitation light source hole and irradiates on the reagent in the consumable, the reagent emits fluorescence with another wavelength to the periphery, part of fluorescence with another wavelength is injected into the fluorescence detection hole, fluorescence with the specified wavelength is obtained by the fluorescence condensing lens group and the second optical filter, the fluorescence with the specified wavelength is collected on the light detection piece, and the conventional nucleic acid amplification detection can be completed by analyzing the intensity of the light.
According to the fluorescence detection device, the consumable carrier can move back and forth and left and right in the same plane through the relative sliding of the first sliding seat and the second sliding seat of the oscillation mechanism, meanwhile, under the driving of the driving motor, the consumable carrier moves on the oscillation mechanism along the circular movement track of the eccentric shaft, and reagents in consumables on the consumable carrier perform rotary movement along the inner wall of the consumable pipe, so that vortex mixing is uniform, amplification efficiency is improved, and accuracy of detection results is further guaranteed.
As shown in fig. 6, the detection module driving mechanism includes a pair of brackets 21 fixed on the base plate 1 at intervals, a fifth guide bar 22 and a sixth guide bar 23 arranged in parallel between the pair of brackets 21, a third slider 24 slidably sleeved on the fifth guide bar and the sixth guide bar, and a driving device 25 driving the third slider, a wire rail 26 is arranged on the third slider 24 perpendicularly to the horizontal direction of the fifth guide bar and the sixth guide bar, and a support frame 27 of the detection module 2 is movably arranged on the wire rail 26 by the driving device. Preferably, the driving means 25 is a stepper motor, which can determine the exact position within the range of travel of the detection module. The detection module is enabled to move in the horizontal direction and the vertical direction in the horizontal plane through the detection module driving mechanism, and detection of a plurality of consumable materials on the consumable material carrier can be achieved.
In addition, the number of the excitation light source group, the fluorescence condensing group and the photodiodes is two or more respectively, so that a plurality of consumable materials can be detected simultaneously, the detection speed is increased, and the detection time is shortened.
A speed regulating mechanism is arranged between the driving motor and the main shaft part of the eccentric shaft, and the rotation of the eccentric shaft at different speeds can be realized through the speed regulating mechanism. As shown in fig. 4, the speed regulating mechanism comprises a driving large wheel 54 sleeved at the output end of a driving motor 52, a driven small wheel 55 driven by the driving large wheel 54 through a synchronous belt, and a driven large wheel 56 coaxially arranged with the driven small wheel 55; the driven large wheel 56 drives the main shaft portion 521 of the eccentric shaft via the timing belt. The speed regulating mechanism may also be a gear speed regulating mechanism. The driving motor can be a servo motor, a direct current brushless motor and the like, preferably a stepping motor, and the accurate positioning of the eccentric shaft is realized by controlling the pulse of the stepping motor.
Further, a photoelectric sensor 57 is provided at a position close to the eccentric shaft 52, and a light blocking piece 58 is provided on the eccentric shaft 52, and the start point of the eccentric shaft rotation is determined by the light blocking piece and the photoelectric sensor.
Further, be equipped with heating film, with heating film electric connection's thermistor and temperature detect switch in the consumptive material carrier 4, can carry out temperature control to reagent in consumptive material carrier, consumptive material and the consumptive material.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (8)
1. The fluorescent detection device with oscillation is characterized by comprising a substrate, a fluorescent detection module arranged on the substrate, an oscillation mechanism arranged above the fluorescent detection module, a consumable carrier arranged on the oscillation mechanism and a driving mechanism;
the fluorescence detection module comprises at least one excitation light source group, at least one fluorescence condensing group and at least one light detection piece, wherein the excitation light source group comprises an LED light source, an excitation light source condensing lens group and a light filter, and the fluorescence condensing group comprises a fluorescence condensing lens group and a second light filter;
the oscillating mechanism comprises a first supporting plate, a second supporting plate, a first guide rod, a second guide rod, a first sliding seat and a second sliding seat, wherein the first supporting plate and the second supporting plate are oppositely arranged at two sides of the fluorescence detection module, the first guide rod and the second guide rod are respectively fixed between two end parts of the first supporting plate and the second supporting plate, the first sliding seat is slidably sleeved on the first guide rod and the second guide rod, the first sliding seat is provided with a third guide rod and a fourth guide rod which are perpendicular to the horizontal direction of the first guide rod and the second guide rod, and the second sliding seat is slidably sleeved on the third guide rod and the fourth guide rod;
the consumable carrier is arranged on the second sliding seat;
the driving mechanism comprises a connecting rod, an eccentric shaft and a driving motor, one end of the connecting rod is fixedly connected with one side of the consumable carrier, the other end of the connecting rod is sleeved at one end of the eccentric shaft, the other end of the eccentric shaft is connected with the driving motor, the eccentric shaft comprises a main shaft part and an eccentric shaft part which is arranged at one end of the main shaft part and is offset relative to the axis of the main shaft part, the main shaft part of the eccentric shaft is vertically and rotatably arranged on the shaft bracket through a bearing, the driving motor is vertically arranged on a base through a motor bracket, the output end of the driving motor is connected with the lower end of the main shaft part of the eccentric shaft through a synchronous belt, the eccentric shaft part is driven by the driving motor to move along the movement track of the axis of the main shaft part in a circular shape, the connecting rod drives the consumable carrier and the consumable to move on the oscillating mechanism along the movement track of the eccentric shaft, and reagents in the consumable rotate in a vortex manner along the inner wall of the consumable pipe;
and a speed regulating mechanism is arranged between the driving motor and the main shaft part of the eccentric shaft.
2. The fluorescence detection device with oscillation according to claim 1, wherein the speed regulating mechanism comprises a driving large wheel sleeved at the output end of the driving motor, a driven small wheel driven by the driving large wheel through a synchronous belt, and a driven large wheel coaxially arranged with the driven small wheel; the driven large wheel drives the main shaft part of the eccentric shaft through a synchronous belt.
3. A fluorescent detection apparatus with oscillation according to any one of claims 1-2, wherein the driving mechanism is provided with a photosensor near the eccentric axis.
4. The fluorescence detection device with oscillation according to claim 1, wherein a heating film, a thermistor electrically connected with the heating film and a temperature control switch are arranged in the consumable carrier.
5. The fluorescence detection device with oscillation according to claim 1, further comprising a detection module driving mechanism including a pair of brackets fixed to the substrate at intervals, a fifth guide bar and a sixth guide bar disposed in parallel between the pair of brackets, a third slider slidably sleeved on the fifth guide bar and the sixth guide bar, and a driving device driving the third slider to slide along the fifth guide bar and the sixth guide bar; the fluorescence detection module is arranged on the third sliding seat.
6. The fluorescence detection device with oscillation according to claim 5, wherein the third carriage is further provided with a wire rail perpendicular to the horizontal direction of the fifth guide bar and the sixth guide bar, and the detection module is movably disposed on the wire rail.
7. The fluorescence detection device with oscillation according to claim 1, wherein the fluorescence detection module further comprises a support frame, at least one excitation light source hole and at least one fluorescence detection hole are adjacently arranged on the support frame, the one excitation light source group is arranged below the one excitation light source hole, and the one fluorescence light gathering group is arranged below the one fluorescence detection hole.
8. The fluorescence detection device of claim 1 or 7, wherein the consumable carrier is provided with light holes.
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| CN202110382895.XA CN113092433B (en) | 2021-04-09 | 2021-04-09 | Fluorescent detection device with oscillation |
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| CN113088445B (en) * | 2021-04-09 | 2024-07-19 | 广州新成生物科技有限公司 | Full-automatic nucleic acid amplification detection analyzer |
| WO2023178668A1 (en) * | 2022-03-25 | 2023-09-28 | 深圳先进技术研究院 | Microbial growth curve determination apparatus |
| CN115400816A (en) * | 2022-09-09 | 2022-11-29 | 深圳鸣基医疗科技有限公司 | Consumable support and liquid detection device |
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| CN101086478A (en) * | 2006-06-06 | 2007-12-12 | 同济大学 | Fluorescent quantitative detection device for PCR |
| CN200974578Y (en) * | 2006-11-27 | 2007-11-14 | 宋云清 | Bicycle having gear box |
| CN103937896B (en) * | 2014-04-25 | 2018-02-13 | 深圳华因康基因科技有限公司 | A kind of SNP classifying methods and kit |
| CN110699243B (en) * | 2019-11-11 | 2022-09-23 | 西南医科大学附属医院 | Multifunctional nucleic acid detection device |
| CN111398614A (en) * | 2020-04-17 | 2020-07-10 | 天津博硕科技有限公司 | Mechanism for chemiluminescence incubation oscillation |
| CN111518872A (en) * | 2020-04-28 | 2020-08-11 | 上海勇求实业发展有限公司 | Method for instant detection of nucleic acid |
| CN112442444A (en) * | 2020-05-30 | 2021-03-05 | 杭州天微基因科技有限公司 | Novel nucleic acid amplification optical detection system |
| CN112175809A (en) * | 2020-10-27 | 2021-01-05 | 广州和实生物技术有限公司 | Integrated nucleic acid detection workstation |
| CN214668592U (en) * | 2021-04-09 | 2021-11-09 | 广州新成生物科技有限公司 | Novel fluorescence detection device with oscillation |
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