CN110699243B - Multifunctional nucleic acid detection device - Google Patents
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- CN110699243B CN110699243B CN201911092524.7A CN201911092524A CN110699243B CN 110699243 B CN110699243 B CN 110699243B CN 201911092524 A CN201911092524 A CN 201911092524A CN 110699243 B CN110699243 B CN 110699243B
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- C12Q1/6844—Nucleic acid amplification reactions
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Abstract
The invention provides a multifunctional nucleic acid detection device which comprises two accommodating cylinders, a driving device, a reaction cylinder, a linkage frame, a rack, a detection box, a detection instrument mounting frame and a whole machine support, wherein the two accommodating cylinders are symmetrically connected to two ends of the whole machine support; the driving device is connected to the whole machine bracket; the driving device is in transmission connection with the linkage frame and the two accommodating cylinders; the linkage frame is movably connected to the whole machine bracket; the reaction cylinder is connected to the linkage frame; the reaction cylinder is in meshed transmission connection with the two racks, and the two racks are symmetrically fixed at two ends of the whole machine bracket; the multifunctional nucleic acid detection device integrates preservation, shaking-up pretreatment, mixing reaction and sampling detection of a nucleic acid sample and an amplification reaction solution, has rich functions, and can effectively improve the detection and treatment efficiency of nucleic acid.
Description
Technical Field
The invention relates to the technical field of detection equipment, in particular to a multifunctional nucleic acid detection device.
Background
Nucleic acids are biomacromolecules synthesized by the polymerization of many nucleotides, and are one of the most basic substances of life. Nucleic acid is widely present in all animal and plant cells and microorganisms, and nucleic acid in organisms is often combined with protein to form nucleoprotein. Different nucleic acids differ in their chemical composition, nucleotide arrangement order, and the like. Nucleic acids are classified into ribonucleic acids (abbreviated as RNA) and deoxyribonucleic acids (abbreviated as DNA) according to their chemical compositions. DNA is the primary material basis for storing, replicating, and transmitting genetic information.
With the development of science and technology, nucleic acid detection has been increasingly applied to the fields of medical diagnosis, food safety detection and the like. However, the nucleic acid detecting apparatus of the prior art is inconvenient for mixing and processing the nucleic acid sample and the amplification reaction solution.
Disclosure of Invention
The invention aims to provide a multifunctional nucleic acid detection device which integrates preservation, shaking-up pretreatment, mixing reaction and sampling detection of a nucleic acid sample and an amplification reaction solution, has rich functions and can effectively improve the detection and treatment efficiency of nucleic acid.
The invention provides a multifunctional nucleic acid detection device which comprises two accommodating cylinders, a driving device, reaction cylinders, a linkage frame, a rack, a detection box, a detection instrument mounting frame and a complete machine support, wherein the two accommodating cylinders are symmetrically connected to two ends of the complete machine support; the driving device is connected to the whole machine bracket; the driving device is in transmission connection with the linkage frame and the two accommodating cylinders; the linkage frame is movably connected to the whole machine bracket; the reaction cylinder is connected to the linkage frame; the reaction cylinder is in meshed transmission connection with the two racks, and the two racks are symmetrically fixed at two ends of the whole machine bracket; two ends of the reaction cylinder are matched with the two accommodating cylinders and drive and control the two accommodating cylinders; the detection box is connected to the whole machine bracket; the detection box is positioned at the lower end of the middle part of the reaction cylinder; the front end of the complete machine support is connected with the detection instrument mounting rack, and the detection instrument mounting rack is arranged opposite to the detection box; and a nucleic acid detector is arranged on the detection instrument mounting frame.
The driving device comprises a driving motor, a lead screw and a transmission chain wheel fixed on the upper part of the lead screw; the driving motor is arranged on the whole machine bracket; an output shaft of the driving motor is fixedly connected with the lead screw; the transmission chain wheel is connected with the two accommodating cylinders in a transmission manner through a chain.
The accommodating cylinder comprises a cylindrical cylinder body, a cylinder cover, a flow controller, an outer gear ring, a transmission gear, a wheel shaft, a driven chain wheel, an outflow pipe and a side frame; the middle part of the cylindrical barrel is rotatably connected to the side frame, and the side frame is fixed on the whole machine bracket; the upper end of the cylindrical barrel is connected with a barrel cover through threads; a liquid inlet pipe with a pipe cover is arranged in the middle of the cylinder cover; the lower end of the cylindrical barrel is fixedly connected with the outflow pipe; the upper part of the flow controller is connected to the cylinder cover; the lower part of the flow controller is in sealing sliding fit in the outflow pipe; the cylindrical barrel is fixedly connected with the outer gear ring meshed with the transmission gear; the transmission gear and the driven chain wheel are fixedly connected to the wheel shaft which is rotationally matched with the side frame; the transmission chain wheel is connected with two driven chain wheels of the two accommodating cylinders through chain transmission.
And the cylindrical barrel is fixedly connected with an electric heating ring.
The flow controller comprises an inverted cone-shaped flow control head, a flow control column, a transverse plate, a round rod, a tension spring, a round seat and a top pressure plate; the inverted conical flow control head, the flow control column and the transverse plate are fixedly connected from bottom to top in sequence; the flow control column is in sealing sliding fit with a pipe orifice at the upper end inside the outflow pipe; the transverse plate is blocked on the bottom surface inside the cylindrical barrel; one ends of the two round rods are fixedly connected to the transverse plate, and the middle parts of the two round rods are in sliding fit with the cylinder cover; the upper ends of the two round rods are respectively fixed with one round seat; the two round seats are fixedly connected with the cylinder cover through one tension spring respectively; the lower ends of the two inverted cone-shaped flow control heads are respectively fixedly connected with the jacking disc which is in sliding fit with the outflow pipe; and a plurality of through-flow holes are uniformly arranged on the top pressing plate in a surrounding manner.
The linkage frame comprises a lifting transverse frame, a top plate, a rectangular sleeve, a rectangular connecting rod, an L-shaped frame, a bidirectional screw, an adjusting turntable and a tensioning spring; the two lifting cross frames are symmetrically matched at two ends of the whole machine bracket in a sliding manner; two ends of the bidirectional screw are respectively and rotatably connected to the two lifting cross frames; the middle part of the bidirectional screw is symmetrically connected with the cross rods of the two L-shaped frames through threads; the vertical rods of the two L-shaped frames are in sliding fit with the whole machine bracket; two ends of the bidirectional screw are respectively and fixedly connected with the adjusting turnplate; the bidirectional screw is sleeved with the tensioning spring; two ends of the tensioning spring are respectively fixedly connected with the cross rods of the two L-shaped frames; the upper ends of the vertical rods of the two L-shaped frames are respectively fixedly connected with the outer end of one rectangular connecting rod, the inner ends of the two rectangular connecting rods are respectively matched in the rectangular sleeve in a sliding manner, and the two rectangular sleeves are respectively fixed at the two ends of the top plate; the top plate is matched on the lead screw through threads.
The outer side of the reaction cylinder is provided with a semi-cylinder body provided with a conical cylinder, a transverse straight pipe, a bent pipe joint, a rotating gear, a vertical straight pipe and a pipe seat plate; the two half cylinders are provided, the inner sides of the two half cylinders are respectively provided with a rubber sealing ring, and the tops of the two rubber sealing rings are in press fit; the outer sides of the two half cylinders are respectively fixedly connected with the inner end of one transverse straight pipe; the outer ends of the two transverse straight pipes are respectively in rotating fit with the lower ends of the two elbow joints through mechanical seals; the upper ends of the two elbow pipe joints are respectively fixedly connected with one vertical straight pipe, and the two vertical straight pipes are respectively fixed on the two lifting cross frames through one pipe seat plate; the two transverse straight pipes are respectively matched on the two lifting transverse frames in a sliding manner; the two transverse straight pipes are respectively and fixedly connected with one rotating gear, and the two rotating gears are in meshed transmission connection with the two racks on the two sides; the two transverse straight pipes are in sealing sliding fit with the two outflow pipes; the upper ends of the two transverse straight pipes are connected with the two pressing plates in a pressing transmission mode.
The detection box comprises a box body with an opening on the top surface, a handle and a T-shaped sliding block; the middle of the bottom end of the box body is in sliding fit in a T-shaped sliding groove of the complete machine bracket through the T-shaped sliding block; the front end of the box body is provided with the handle; the box body is positioned right below the connection position of the inner sides of the two half cylinder bodies; the box body and the detecting instrument mounting frame are arranged oppositely.
The inner side of the box body is provided with a plurality of sample grooves.
The lower ends of the plurality of sample grooves are communicated through a drainage groove; the inner side of the drainage groove is connected with a plugging plate in a sealing sliding fit manner; the top surface of the plugging plate is provided with a test strip placing groove.
The embodiment of the invention has the following beneficial effects: the invention aims at providing a multifunctional nucleic acid detection device which integrates preservation, shaking-up pretreatment, mixed reaction and sampling detection of a nucleic acid sample and an amplification reaction solution, has rich functions and can effectively improve the detection treatment efficiency of nucleic acid; the internal driving device is adopted for driving, the two containing cylinders can be driven to shake up the nucleic acid sample or the amplification reaction solution or other reaction solutions in the containing cylinders, the uniformity of all substances in the nucleic acid sample or the amplification reaction solution or other reaction solutions is improved when the nucleic acid sample or the amplification reaction solution or other reaction solutions in the containing cylinders are sampled, and the detection accuracy after mixed reaction is improved; the driving device in the reaction barrel can drive the reaction barrel to extract a sample from a nucleic acid sample or amplification reaction liquid or other reaction solution in the two accommodating barrels, the mixing can be realized when the sample is extracted, the sample extraction speed can be adjusted according to the requirement, different requirements can be met, the nucleic acid sample and the amplification reaction liquid or other reaction solution are fully and uniformly mixed, the amplification reaction efficiency is improved, and the detection accuracy is improved; the inside detection box that can be convenient for install or dismantle nucleic acid test paper that is equipped with, the nucleic acid detection appearance of being convenient for the cooperation installation on the detecting instrument mounting bracket carries out dual detection, improves and detects the accuracy.
In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present invention or technical solutions in related arts, the drawings used in the description of the embodiments or related arts will be briefly described below, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a first general schematic diagram provided in accordance with an embodiment of the present invention;
FIG. 2 is a second overall view provided in accordance with an embodiment of the present invention;
FIG. 3 is a schematic view of a receiving canister according to an embodiment of the present invention;
fig. 4 is a schematic diagram of a flow controller according to an embodiment of the present invention;
fig. 5 is a schematic diagram of a driving device according to an embodiment of the present invention.
FIG. 6 is a schematic view of a reaction cartridge provided in an embodiment of the present invention;
FIG. 7 is a schematic view of a linkage mount provided in accordance with an embodiment of the present invention;
FIG. 8 is a schematic view of a rack provided by an embodiment of the present invention;
FIG. 9 is a first schematic view of a cartridge according to an embodiment of the present invention;
FIG. 10 is a schematic view of a test instrument mounting block provided in accordance with an embodiment of the present invention;
FIG. 11 is a schematic view of a complete machine support according to an embodiment of the present invention;
FIG. 12 is a second schematic view of a detecting box according to an embodiment of the present invention.
Icon: an accommodating cylinder 1; a cylindrical barrel 101; a cartridge cover 102; a flow controller 103; an inverted cone-shaped flow control head 103A; a flow control column 103B; a transverse plate 103C; a round bar 103D; a tension spring 103E; a circular seat 103F; a pressing plate 103G; an outer ring gear 104; a transmission gear 105; an axle 106; a driven sprocket 107; an outflow tube 108; a side frame 109; a drive device 2; a drive motor 201; a lead screw 202; a drive sprocket 203; a reaction cylinder 3; a half cylinder 301; a transverse straight tube 302; an elbow fitting 303; a rotary gear 304; a vertical straight pipe 305; a tube seat plate 306; a linkage frame 4; a lifting cross frame 401; a top plate 402; a rectangular sleeve 403; a rectangular link 404; an L-shaped frame 405; a bi-directional screw 406; an adjustment dial 407; a tension spring 408; a rack 5; a detection cassette 6; a case 601; a handle 602; a T-shaped slider 603; a sample tank 604; a plugging plate 605; a detection instrument mounting frame 7; and a complete machine bracket 8.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are only some embodiments, but not all embodiments, of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in a specific case to those of ordinary skill in the art.
The present invention is described in further detail below with reference to figures 1-12.
The first embodiment is as follows:
as shown in fig. 1-12, a multifunctional nucleic acid detecting device comprises two accommodating cylinders 1, a driving device 2, a reaction cylinder 3, a linkage frame 4, a rack 5, a detecting box 6, a detecting instrument mounting frame 7 and a complete machine support 8, wherein the two accommodating cylinders 1 are symmetrically connected to two ends of the complete machine support 8; the driving device 2 is connected to the whole machine bracket 8; the driving device 2 is in transmission connection with the linkage frame 4 and the two accommodating cylinders 1; the linkage frame 4 is movably connected to the whole machine bracket 8; the reaction cylinder 3 is connected to the linkage frame 4; the reaction cylinder 3 is in meshed transmission connection with the two racks 5, and the two racks 5 are symmetrically fixed at two ends of the whole machine bracket 8; two ends of the reaction cylinder 3 are matched with the two accommodating cylinders 1, and the two accommodating cylinders 1 are driven and controlled; the detection box 6 is connected to the whole machine bracket 8; the detection box 6 is positioned at the lower end of the middle part of the reaction cylinder 3; the front end of the whole machine support 8 is connected with the detection instrument mounting rack 7, and the detection instrument mounting rack 7 is arranged opposite to the detection box 6; and a nucleic acid detector is arranged on the detection instrument mounting frame 7.
The invention relates to a multifunctional nucleic acid detection device, which is provided with two containing cylinders 1, wherein a nucleic acid sample is filled in one containing cylinder 1 for preservation or pretreatment, an amplification reaction liquid or other liquid for carrying out amplification reaction on the nucleic acid sample is arranged in the other containing cylinder 1, a driving device 2 is switched on and started on the start-up detection, and the driving device 2 can drive the two containing cylinders 1 to shake up the nucleic acid sample or the amplification reaction liquid or other reaction solution in the two containing cylinders after being started, so that the uniformity of each substance in the nucleic acid sample or the amplification reaction liquid or other reaction solution is improved when the nucleic acid sample or the amplification reaction liquid or other reaction solution in the containing cylinder 1 is sampled, and the detection accuracy after mixed reaction is improved; the driving device 2 in the reaction barrel can drive the reaction barrel 3 to be matched with the rack 5 to extract a sample from a nucleic acid sample, an amplification reaction solution or other reaction solutions in the two accommodating barrels 1, the mixing can be realized during the sample extraction, the sample extraction speed can be adjusted according to the requirement, different requirements are met, the nucleic acid sample and the amplification reaction solution or other reaction solutions are fully and uniformly mixed, the amplification reaction efficiency is improved, and the detection accuracy is improved; the nucleic acid sample after the mixed reaction can fall into the detection box 6 for detection by opening the reaction cylinder 3, the position of the detection box 6 can be adjusted and can slide to the lower end of the nucleic acid detector on the detection instrument mounting frame 7 for detection, the detection box 6 which can facilitate the installation or the disassembly of the nucleic acid detection test paper is arranged in the detection box, the double detection is facilitated by matching with the nucleic acid detector installed on the detection instrument mounting frame 7, and the detection accuracy is improved; the nucleic acid detector can be an externally purchased conventional nucleic acid detector.
The second embodiment is as follows:
as shown in fig. 1 to 12, the driving device 2 includes a driving motor 201, a lead screw 202, and a driving sprocket 203 fixed on the upper portion of the lead screw 202; the driving motor 201 is installed on the whole machine bracket 8; an output shaft of the driving motor 201 is fixedly connected with a lead screw 202; the transmission chain wheel 203 is connected with the two accommodating cylinders 1 through chain transmission. The driving motor 201 can drive the screw rod 202 to rotate forward or reversely after being started, the screw rod 202 can drive the linkage frame 4 to move up and down on the whole machine support 8 when rotating forward or reversely, the linkage frame 4 drives the reaction cylinder 3 to extract a sample from a nucleic acid sample or amplification reaction liquid or other reaction solution in the two accommodating cylinders 1 when moving up, the mixing can be realized when the sample is extracted, the sample extraction speed can be adjusted according to the requirement, different requirements can be met, the nucleic acid sample and the amplification reaction liquid or other reaction solution can be fully and uniformly mixed, the amplification reaction efficiency is improved, and the detection accuracy is improved; otherwise, the sampling operation is stopped and the mixed reaction treatment is carried out.
The third concrete implementation mode:
as shown in fig. 1-12, the accommodating cylinder 1 comprises a cylindrical cylinder body 101, a cylinder cover 102, a flow controller 103, an outer gear ring 104, a transmission gear 105, a wheel axle 106, a driven sprocket 107, an outlet pipe 108 and a side frame 109; the middle part of the cylindrical barrel 101 is rotatably connected to the side frame 109, and the side frame 109 is fixed on the whole machine bracket 8; the upper end of the cylindrical barrel 101 is connected with a barrel cover 102 through threads; a liquid inlet pipe with a pipe cover is arranged in the middle of the cylinder cover 102; the lower end of the cylindrical barrel 101 is fixedly connected with the outflow pipe 108; the upper part of the flow controller 103 is connected to the cylinder cover 102; the lower portion of the flow controller 103 is a sealed sliding fit within the outlet tube 108; the outer gear ring 104 meshed with the transmission gear 105 is fixedly connected to the cylindrical barrel 101; the transmission gear 105 and the driven sprocket 107 are fixedly connected to the wheel shaft 106 which is rotatably matched on the side frame 109; the driving chain wheel 203 is connected with the two driven chain wheels 107 of the two accommodating cylinders 1 through chain transmission. When the transmission chain wheel 203 rotates, the driven chain wheels 107 of the two containing cylinders 1 can be driven to rotate through chain transmission, the driven chain wheels 107 can drive the wheel shafts 106 and the transmission gears 105 to rotate, the transmission gears 105 can drive the outer gear rings 104 to rotate, the outer gear rings 104 can drive the cylindrical cylinder body 101 to rotate, and the solution in the cylindrical cylinder body 101 can be rotated and shaken uniformly in the rotating process without being filled with the solution; flow control device 103 and outflow tube 108 cooperate with reaction cylinder 3 to control the flow during sampling, reaction cylinder 3 pushes up flow control device 103 upwards to make the inside nucleic acid sample of cylinder barrel 101 or other solutions accessible outflow tube 108 enter into reaction cylinder 3 in to can adjust the speed of sample, use when satisfying different demands.
The fourth concrete implementation mode:
as shown in fig. 1 to 12, an electric heating ring is fixedly connected to the cylindrical barrel 101. The electric heating ring heats the interior of the cylindrical barrel body 101, so that preservation pretreatment of nucleic acid samples or other solutions at different temperatures can be conveniently carried out during pretreatment.
The fifth concrete implementation mode:
as shown in fig. 1-12, the flow controller 103 comprises an inverted cone-shaped flow control head 103A, a flow control column 103B, a horizontal plate 103C, a round bar 103D, a tension spring 103E, a round seat 103F and a pressing plate 103G; the inverted conical flow control head 103A, the flow control column 103B and the transverse plate 103C are fixedly connected from bottom to top in sequence; the flow control column 103B is in sealing sliding fit at the upper end pipe orifice inside the outflow pipe 108; the transverse plate 103C is blocked on the bottom surface inside the cylindrical barrel 101; one ends of the two round rods 103D are fixedly connected to the transverse plate 103C, and the middle parts of the two round rods 103D are in sliding fit with the cylinder cover 102; the upper ends of the two round rods 103D are respectively fixed with one round seat 103F; the two round seats 103F are respectively fixedly connected with the cylinder cover 102 through one tension spring 103E; the lower ends of the two inverted cone-shaped flow control heads 103A are respectively fixedly connected with the top pressing disc 103G which is in sliding fit in the outflow pipe 108; a plurality of through holes are uniformly arranged on the top pressing plate 103G in a surrounding manner.
When the reaction cylinder 3 pushes the top pressing plate 103G upwards, the top pressing plate 103G drives the inverted cone-shaped flow control head 103A and the flow control column 103B to move upwards, when the inverted cone-shaped flow control head 103A and the flow control column 103B move upwards until the flow control column 103B is separated from the upper end orifice of the outflow tube 108, a nucleic acid sample or other reaction solution in the cylindrical cylinder 101 can flow out through a gap between the inverted cone-shaped flow control head 103A and the outflow tube 108 and enter the reaction cylinder 3 through the outflow tube 108, the gap between the inverted cone-shaped flow control head 103A and the outflow tube 108 is increased along with the upward movement of the inverted cone-shaped flow control head 103A to realize flow rate adjustment, at this time, the tension spring 103E is in a stretching state, when the reaction cylinder 3 is gradually separated from the top pressing plate 103G downwards, the inverted cone-shaped flow control head 103A and the flow control column 103B return to original positions under the effect of elasticity and are blocked in the outflow tube 108 to prevent the nucleic acid sample or other reaction solution in the cylindrical cylinder 101 from flowing out, the function of flow control is played.
The sixth specific implementation mode:
as shown in fig. 1 to 12, the linkage frame 4 comprises a lifting cross frame 401, a top plate 402, a rectangular sleeve 403, a rectangular connecting rod 404, an L-shaped frame 405, a bidirectional screw 406, an adjusting turntable 407 and a tension spring 408; two lifting cross frames 401 are arranged, and the two lifting cross frames 401 are symmetrically matched with two ends of the whole machine support 8 in a sliding manner; two ends of the bidirectional screw 406 are respectively and rotatably connected to the two lifting cross frames 401; the middle part of the bidirectional screw rod 406 is symmetrically connected with the cross rods of the two L-shaped frames 405 through threads; the vertical rods of the two L-shaped frames 405 are in sliding fit with the whole machine bracket 8; two ends of the bidirectional screw 406 are respectively and fixedly connected with one adjusting turntable 407; the tension spring 408 is sleeved on the bidirectional screw rod 406; two ends of the tension spring 408 are respectively and fixedly connected with the cross bars of the two L-shaped frames 405; the upper ends of the vertical rods of the two L-shaped frames 405 are respectively and fixedly connected with the outer ends of one rectangular connecting rod 404, the inner ends of the two rectangular connecting rods 404 are respectively and slidably matched in one rectangular sleeve 403, and the two rectangular sleeves 403 are respectively fixed at the two ends of the top plate 402; the top plate 402 is threadedly engaged on the lead screw 202. The top plate 402 in the linkage frame 4 moves up and down under the driving of the lead screw 202, the top plate 402 drives the two L-shaped frames 405 to move up and down through the two rectangular sleeves 403 and the two rectangular connecting rods 404, the two L-shaped frames 405 drive the two-way screw 406, the adjusting turntable 407, the tension spring 408 and the lifting cross frame 401 to move up and down, and the two lifting cross frames 401 slide on the whole machine support 8 to play a role in guiding and limiting; the reaction cylinder 3 is driven to move up and down by the two L-shaped frames 405 and the two lifting cross frames 401; the rotation of the adjusting turntable 407 can drive the bidirectional screw 406 to rotate, the rotation of the bidirectional screw 406 can drive the two rectangular connecting rods 404 to perform relative motion or deviation motion, and the two rectangular connecting rods 404 drive the two half cylinders 301 in the reaction cylinder 3 to perform contact closing or separation opening work; the two rectangular connecting rods 404 slide in the two rectangular sleeves 403, so that the normal operation is not influenced; the provision of the tension spring 408 improves the stability of the two half-cylinders 301 when brought into contact closure or separation.
The seventh embodiment:
as shown in fig. 1-12, a half cylinder 301 provided with a conical cylinder, a horizontal straight pipe 302, an elbow joint 303, a rotary gear 304, a vertical straight pipe 305 and a pipe seat plate 306 are arranged outside the reaction cylinder 3; the number of the half cylinder bodies 301 is two, the inner sides of the two half cylinder bodies 301 are respectively provided with a rubber sealing ring, and the tops of the two rubber sealing rings are in press fit; the outer sides of the two half cylinders 301 are respectively fixedly connected with the inner end of one transverse straight pipe 302; the outer ends of the two transverse straight pipes 302 are respectively in rotating fit with the lower ends of the two elbow joints 303 through mechanical sealing; the upper ends of the two elbow joints 303 are respectively and fixedly connected with one vertical straight pipe 305, and the two vertical straight pipes 305 are respectively fixed on the two lifting crossbearers 401 through one pipe seat plate 306; the two transverse straight pipes 302 are respectively matched on the two lifting transverse frames 401 in a sliding manner; the two transverse straight pipes 302 are respectively and fixedly connected with one rotating gear 304, and the two rotating gears 304 are in meshed transmission connection with the two racks 5 on the two sides; the two transverse straight pipes 302 are in sealing sliding fit with the two outflow pipes 108; the upper ends of the two transverse straight pipes 302 are connected with the two pressing discs 103G in a pressing transmission mode. The two transverse straight pipes 302 move up and down under the drive of the two L-shaped frames 405 and the two lifting transverse frames 401; the two transverse straight pipes 302 drive the reaction cylinder 3 to move up and down integrally, when the two vertical straight pipes 305 move up, the two vertical straight pipes 305 can slide in the two outflow pipes 108 in a sealing manner and can be used for jacking the jacking plate 103G, so that sampling work is realized, and a nucleic acid sample or amplification reaction liquid enters the half cylinder body 301 through the vertical straight pipes 305, the elbow joint 303 and the transverse straight pipes 302 to be mixed; when the two rotary gears 304 move up and down to contact with the two racks 5, the two rotary gears 304 can be driven to rotate around the axes of the two rotary gears 304, when the two rotary gears 304 rotate, the two half cylinders 301 can be driven to rotate through the two transverse straight pipes 302, and then the nucleic acid sample and the amplification reaction liquid or other reaction liquid entering the two rotary half cylinders 301 are mixed; the rotation of the adjusting turntable 407 can drive the bidirectional screw 406 to rotate, the rotation of the bidirectional screw 406 can drive the two rectangular connecting rods 404 to perform relative motion or deviation motion, and the two rectangular connecting rods 404 drive the two half cylinders 301 in the reaction cylinder 3 to perform contact closing or separation opening work; when the detection kit is opened, the sample solution after the internal reaction can enter the detection kit 6 for subsequent detection.
The specific implementation mode is eight:
as shown in FIGS. 1 to 12, the cartridge 6 includes a case body 601 with an open top surface, a handle 602, and a T-shaped slider 603; the middle of the bottom end of the box body 601 is in sliding fit in a T-shaped sliding groove of the whole machine bracket 8 through the T-shaped sliding block 603; the front end of the box body 601 is provided with the handle 602; the box body 601 is positioned right below the connection position of the inner sides of the two half cylinder bodies 301; the box body 601 is arranged opposite to the detection instrument mounting frame 7.
The inner side of the box body 601 is provided with a plurality of sample grooves 604.
The lower ends of the plurality of sample grooves 604 are communicated through a drainage groove; the inner side of the drainage groove is connected with a plugging plate 605 in a sealing and sliding fit manner; the top surface of the plugging plate 605 is provided with a test strip placing groove.
The nucleic acid sample after the mixed reaction can fall into the detection box 6 for detection by opening the reaction cylinder 3, the position of the detection box 6 can be adjusted and can slide to the lower end of the nucleic acid detector on the detection instrument mounting frame 7 for detection, the detection box 6 which can facilitate the installation or the disassembly of the nucleic acid detection test paper is arranged in the detection box, the double detection is facilitated by matching with the nucleic acid detector installed on the detection instrument mounting frame 7, and the detection accuracy is improved; the arrangement of the blocking plate 605 is convenient for taking and placing the test strip, discharging a plurality of sample solutions in the sample tank 604, and cleaning the inner side of the box body 601 by matching with a cleaning solution.
The principle of the invention is as follows: the invention relates to a multifunctional nucleic acid detection device, which is provided with two containing cylinders 1, wherein a nucleic acid sample is filled in one containing cylinder 1 for preservation or pretreatment, an amplification reaction liquid or other liquid for carrying out amplification reaction on the nucleic acid sample is arranged in the other containing cylinder 1, a driving device 2 is switched on and started on the start-up detection, and the driving device 2 can drive the two containing cylinders 1 to shake up the nucleic acid sample or the amplification reaction liquid or other reaction solution in the two containing cylinders after being started, so that the uniformity of each substance in the nucleic acid sample or the amplification reaction liquid or other reaction solution is improved when the nucleic acid sample or the amplification reaction liquid or other reaction solution in the containing cylinder 1 is sampled, and the detection accuracy after mixed reaction is improved; the driving device 2 in the reaction barrel can drive the reaction barrel 3 to be matched with the rack 5 to extract a sample from a nucleic acid sample, an amplification reaction solution or other reaction solutions in the two accommodating barrels 1, the mixing can be realized during the sample extraction, the sample extraction speed can be adjusted according to the requirement, different requirements are met, the nucleic acid sample and the amplification reaction solution or other reaction solutions are fully and uniformly mixed, the amplification reaction efficiency is improved, and the detection accuracy is improved; the nucleic acid sample after the mixed reaction can fall into the detection box 6 for detection by opening the reaction cylinder 3, the position of the detection box 6 can be adjusted and can slide to the lower end of the nucleic acid detector on the detection instrument mounting frame 7 for detection, the detection box 6 which can facilitate the installation or the disassembly of the nucleic acid detection test paper is arranged in the detection box, the double detection is facilitated by matching with the nucleic acid detector installed on the detection instrument mounting frame 7, and the detection accuracy is improved; the nucleic acid detector can be an externally purchased conventional nucleic acid detector.
Finally, it should be noted that: the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and these modifications or substitutions do not depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (8)
1. The utility model provides a multi-functional nucleic acid detection device, includes a holding section of thick bamboo (1), drive arrangement (2), reaction cylinder (3), linkage frame (4), rack (5), detects box (6), detecting instrument mounting bracket (7) and complete machine support (8), its characterized in that: the number of the containing cylinders (1) is two, and the two containing cylinders (1) are symmetrically connected to two ends of the whole machine support (8); the driving device (2) is connected to the whole machine bracket (8); the driving device (2) is in transmission connection with the linkage frame (4) and the two accommodating cylinders (1); the linkage frame (4) is movably connected to the whole machine bracket (8); the reaction cylinder (3) is connected to the linkage frame (4); the reaction cylinder (3) is in meshed transmission connection with the two racks (5), and the two racks (5) are symmetrically fixed at two ends of the whole machine bracket (8); two ends of the reaction cylinder (3) are matched with the two accommodating cylinders (1) and drive and control the two accommodating cylinders (1); the detection box (6) is connected to the whole machine bracket (8); the detection box (6) is positioned at the lower end of the middle part of the reaction cylinder (3); the front end of the whole machine support (8) is connected with the detection instrument mounting rack (7), and the detection instrument mounting rack (7) is arranged opposite to the detection box (6); a nucleic acid detector is arranged on the detection instrument mounting frame (7);
the driving device (2) comprises a driving motor (201), a lead screw (202) and a transmission chain wheel (203) fixed on the upper part of the lead screw (202); the driving motor (201) is arranged on the whole machine bracket (8); an output shaft of the driving motor (201) is fixedly connected with a lead screw (202); the transmission chain wheel (203) is in transmission connection with the two accommodating cylinders (1) through a chain;
the accommodating cylinder (1) comprises a cylindrical cylinder body (101), a cylinder cover (102), a flow controller (103), an outer gear ring (104), a transmission gear (105), a wheel shaft (106), a driven chain wheel (107), an outflow pipe (108) and a side frame (109); the middle part of the cylindrical barrel (101) is rotatably connected to the side frame (109), and the side frame (109) is fixed on the whole machine bracket (8); the upper end of the cylindrical barrel body (101) is connected with a barrel cover (102) through threads; a liquid inlet pipe with a pipe cover is arranged in the middle of the cylinder cover (102); the lower end of the cylindrical barrel (101) is fixedly connected with the outflow pipe (108); the upper part of the flow controller (103) is connected to the cylinder cover (102); the lower part of the flow controller (103) is in sealed sliding fit in the outflow pipe (108); the cylindrical barrel (101) is fixedly connected with the outer gear ring (104) meshed with the transmission gear (105); the transmission gear (105) and the driven chain wheel (107) are fixedly connected to the wheel shaft (106) which is rotationally matched on the side frame (109); the transmission chain wheel (203) is connected with two driven chain wheels (107) of the two accommodating cylinders (1) through chain transmission.
2. The multifunctional nucleic acid detecting apparatus according to claim 1, wherein: the cylindrical barrel (101) is fixedly connected with an electric heating ring.
3. The multifunctional nucleic acid detecting apparatus according to claim 2, wherein: the flow controller (103) comprises an inverted cone-shaped flow control head (103A), a flow control column (103B), a transverse plate (103C), a round rod (103D), a tension spring (103E), a round seat (103F) and a top pressing plate (103G); the inverted-cone-shaped flow control head (103A), the flow control column (103B) and the transverse plate (103C) are fixedly connected from bottom to top in sequence; the flow control column (103B) is in sealing sliding fit with an upper end pipe orifice inside the outflow pipe (108); the transverse plate (103C) is blocked on the bottom surface inside the cylindrical barrel (101); one ends of the two round rods (103D) are fixedly connected to the transverse plate (103C), and the middle parts of the two round rods (103D) are in sliding fit with the cylinder cover (102); the upper ends of the two round rods (103D) are respectively fixed with one round seat (103F); the two round seats (103F) are respectively fixedly connected with the cylinder cover (102) through one tension spring (103E); the lower ends of the two inverted cone-shaped flow control heads (103A) are respectively fixedly connected with the top pressing disc (103G) which is in sliding fit with the outflow pipe (108); a plurality of through-flow holes are uniformly arranged on the top pressing plate (103G) in a surrounding manner.
4. The multifunctional nucleic acid detecting apparatus according to claim 3, wherein: the linkage frame (4) comprises a lifting transverse frame (401), a top plate (402), a rectangular sleeve (403), a rectangular connecting rod (404), an L-shaped frame (405), a bidirectional screw (406), an adjusting turntable (407) and a tensioning spring (408); two lifting cross frames (401) are arranged, and the two lifting cross frames (401) are symmetrically matched with two ends of the whole machine support (8) in a sliding manner; two ends of the bidirectional screw (406) are respectively and rotatably connected to the two lifting cross frames (401); the middle part of the bidirectional screw (406) is symmetrically connected with the cross bars of the two L-shaped frames (405) through threads; the vertical rods of the two L-shaped frames (405) are in sliding fit with the whole machine bracket (8); two ends of the bidirectional screw (406) are respectively and fixedly connected with the adjusting turnplate (407); the bidirectional screw (406) is sleeved with the tension spring (408); two ends of the tensioning spring (408) are respectively and fixedly connected with the cross bars of the two L-shaped frames (405); the upper ends of the vertical rods of the two L-shaped frames (405) are respectively fixedly connected with the outer end of one rectangular connecting rod (404), the inner ends of the two rectangular connecting rods (404) are respectively in sliding fit in one rectangular sleeve (403), and the two rectangular sleeves (403) are respectively fixed at the two ends of the top plate (402); the top plate (402) is screwed on the lead screw (202).
5. The multifunctional nucleic acid detecting apparatus according to claim 4, wherein: the outer side of the reaction cylinder (3) is provided with a semi-cylinder body (301) provided with a conical cylinder, a transverse straight pipe (302), an elbow joint (303), a rotating gear (304), a vertical straight pipe (305) and a pipe seat plate (306); the number of the half cylinders (301) is two, the inner sides of the two half cylinders (301) are respectively provided with a rubber sealing ring, and the tops of the two rubber sealing rings are in press fit; the outer sides of the two half cylinders (301) are respectively fixedly connected with the inner end of one transverse straight pipe (302); the outer ends of the two transverse straight pipes (302) are respectively in rotating fit with the lower ends of the two elbow joints (303) through mechanical sealing; the upper ends of the two elbow joints (303) are respectively fixedly connected with one vertical straight pipe (305), and the two vertical straight pipes (305) are respectively fixed on the two lifting cross frames (401) through one pipe seat plate (306); the two transverse straight pipes (302) are respectively in sliding fit with the two lifting transverse frames (401); the two transverse straight pipes (302) are respectively and fixedly connected with one rotating gear (304), and the two rotating gears (304) are in meshed transmission connection with the two racks (5) on the two sides; the two transverse straight pipes (302) are in sealing sliding fit with the two outflow pipes (108); the upper ends of the two transverse straight pipes (302) are connected with the two pressing plates (103G) in a pressing transmission mode.
6. The multifunctional nucleic acid detecting apparatus according to claim 5, wherein: the detection box (6) comprises a box body (601) with an opening on the top surface, a handle (602) and a T-shaped sliding block (603); the middle of the bottom end of the box body (601) is in sliding fit in a T-shaped sliding groove of the whole machine bracket (8) through the T-shaped sliding block (603); the front end of the box body (601) is provided with the handle (602); the box body (601) is positioned right below the connection position of the inner sides of the two half cylinders (301); the box body (601) is arranged opposite to the detection instrument mounting frame (7).
7. The multifunctional nucleic acid detecting apparatus according to claim 6, wherein: the inner side of the box body (601) is provided with a plurality of sample grooves (604).
8. The multifunctional nucleic acid detecting apparatus according to claim 7, wherein: the lower ends of a plurality of sample grooves (604) are communicated through a drainage groove; the inner side of the drainage groove is connected with a plugging plate (605) in a sealing and sliding fit manner; the top surface of the blocking plate (605) is provided with a test paper strip placing groove.
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