CN105759066B - Full automatic blood-coagulation detection analysis instrument, method - Google Patents
Full automatic blood-coagulation detection analysis instrument, method Download PDFInfo
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- CN105759066B CN105759066B CN201610268795.3A CN201610268795A CN105759066B CN 105759066 B CN105759066 B CN 105759066B CN 201610268795 A CN201610268795 A CN 201610268795A CN 105759066 B CN105759066 B CN 105759066B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
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- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The present invention provides a kind of full automatic blood-coagulation detection analysis instrument, method, and the analyzer includes babinet, sample-adding module, cleaning module, detection module and analysis module；The detection module includes：Micro-fluidic disc, the micro-fluidic disc have inlet, reactive tank and test chamber；Motor, the motor are used to that the disc to be driven to rotate；Light source, the light source are used to send measurement light；Detector, the detector is used to the measurement light after being absorbed by sample in the test chamber being converted to electric signal, and send the analysis module；Heating member, the heating member are used to heat the micro-fluidic disc；Bracket, the bracket are equipped with guide rail, and the micro-fluidic disc, motor are mounted on the bracket；Slip lid, the slip lid are arranged on the guide rail, and the micro-fluidic disc is blocked when the slip lid moves.The present invention has many advantages, such as simple in structure, full-automatic.
Description
Technical field
The present invention relates to the analysis of photo-electric hemostasis examination, methods, also can be extended to the biochemistry detection analysis of other photo-electrics.
Background technology
Blood coagulation measuring and analysing meter is the detection instrument that hospital is used for detecting patient's clotting time and coagulation function.At present, exist
In blood coagulation tester device field, there is full automatic blood coagulation tester, also there is automanual blood coagulation tester, full automatic blood-coagulation test
The implementation of instrument is using fixed partition, then reinjects blood sample and test agent, since subregion is more, to complete entirely certainly
Dynamic detection process causes multiple XYZ kinematic systems intersections frequently to run on each separation fixed interval, so also makes entire instrument
Test speed is slow, and whole device is huge cumbersome, and measuring accuracy is not high, but also is not easily controlled, and high failure rate repair is difficult
Degree is big, and cost is higher；Semi-automatic blood coagulation tester then needs to expend larger human cost, and it is more not adapt to test sample amount
It is required with testing time short wait, full automatic blood-coagulation tester is suitable for the test of great amount of samples, although semi-automatic blood coagulation tester
It is suitble to the test of a small amount of sample, but expends more manpower and time.
The content of the invention
In order to solve the deficiency in above-mentioned prior art, the present invention provides a kind of detection and analysis of full automatic blood-coagulation
Instrument, have detection is accurate, sample-adding is accurate, save reagent, reagent holding time length, detection process heated at constant temperature, detection efficiency height,
Simple operation and other advantages.
The purpose of the present invention is what is be achieved through the following technical solutions：
A kind of full automatic blood-coagulation detection analysis instrument, the full automatic blood-coagulation detection analysis instrument include babinet, are loaded module, are clear
Mold cleaning block, detection module and analysis module；The detection module includes：
Micro-fluidic disc, the micro-fluidic disc have inlet, reactive tank and test chamber；
Motor, the motor are used to that the disc to be driven to rotate；
Light source, the light source are used to send measurement light；
Detector, the detector are used to the measurement light after being absorbed by sample in the test chamber being converted to electric signal,
And send the analysis module；
Heating member, the heating member are used to heat the micro-fluidic disc；
Bracket, the bracket are equipped with guide rail, and the micro-fluidic disc, motor are mounted on the bracket；
Slip lid, the slip lid are arranged on the guide rail, and the micro-fluidic disc is blocked when the slip lid moves.
According to above-mentioned full automatic blood-coagulation detection analysis instrument, optionally, the detection module further comprises：
At least two optical fiber, the light input end of at least two optical fiber connect an only light source, and light output end is in
It is separately fixed at the upside or downside of the disc arc；
The quantity of the detector and the quantity Matching of the light output end.
According to above-mentioned full automatic blood-coagulation detection analysis instrument, it is preferable that
The babinet is provided with working panel, and sample position, reagent position, cleaning position are set on the working panel；
The sample-adding module includes three-dimensional moving arm, sample needle.
According to above-mentioned full automatic blood-coagulation detection analysis instrument, it is preferable that at least two light output ends are fixed on the bracket
On, the detector is fixed on the slip lid.
According to above-mentioned full automatic blood-coagulation detection analysis instrument, optionally, the sample-adding module includes：
For X to stent, the X has X direction guiding rails, the first motor, X to conveyer belt and the first rotating wheel, the X on stent
It is wound on to conveyer belt on first motor and the first rotating wheel；
Y-direction stent, the Y-direction stent connect the X and horizontally slip to conveyer belt and on the X direction guiding rails；The Y-direction
There is Y-direction guide rail, the second motor, Y-direction conveyer belt and the second rotating wheel, the Y-direction conveyer belt is wound on second horse on stent
Up to on the second rotating wheel；
Z-direction stent, the Z-direction stent connect the Y-direction conveyer belt and are slid back and forth in the Y-direction guide rail；The Z-direction
There is Z-direction guide rail, the 3rd motor, Z-direction conveyer belt and the 3rd rotating wheel, the Z-direction conveyer belt is wound on the 3rd horse on stent
Up to on the 3rd rotating wheel；
Sample needle, the sample needle connect the Z-direction conveyer belt by connector and are slided up and down on Z-direction guide rail.
According to above-mentioned full automatic blood-coagulation detection analysis instrument, optionally, the slip lid includes：
Cover board, support plate, the cover board are fixed in the support plate, and with through hole；The both sides of the support plate are set
There is the sliding block with the guide rail；
The detector is arranged between cover board and support plate.
According to above-mentioned full automatic blood-coagulation detection analysis instrument, optionally, the full automatic blood-coagulation detection analysis instrument is further
Including：
Pre- thermal potential, the pre- thermal potential are used to preheat the micro-fluidic disc.
According to above-mentioned full automatic blood-coagulation detection analysis instrument, optionally, the reagent position includes：
Mark, the mark is arranged on the working panel, for labelled reagent；
Reagent chamber, the reagent chamber are used to accommodate reagent；
Refrigerator, the refrigerator are used for the reagent to freeze in the reagent chamber；
Radiator, the radiator are thermally connected with the refrigerator；
Air passage, the air passage are thermally connected with the radiator；
Fan, the fan are arranged on the inlet in the air passage.
The present invention also aims to provide a kind of full automatic blood-coagulation determination method namely above-mentioned full automatic blood-coagulation
The method of work of detection analysis instrument.The goal of the invention is achieved by the following technical programs：
According to the method for work of any of the above-described full automatic blood-coagulation detection analysis instrument, the method for work comprises the following steps：
(A1) sample, reagent are put into sample position, reagent position respectively；
(A2) push slip lid open, preheated micro-fluidic disc is put into detection module；
(A3) the slip lid return；Sample-adding module after cleaning extracts quantitative sample, reagent respectively, and injects micro-fluidic
Disc；
(A4) the motor driving micro-fluidic disc rotation, sample and reagent mixing；
Heating member heats the micro-fluidic disc；
(A5) the measurement light that light source is sent send analysis mould through the mixture in test chamber, the electric signal of detector output
Block；
(A6) analysis module handles the electric signal, so as to know the parameter of sample.
According to above-mentioned method of work, optionally, the method for work is further comprising the steps of：
(B1) reagent in refrigerator refrigeration reagent chamber；
(B2) heat that the refrigerator generates is distributed with the hot linked radiator of the refrigerator；
(B3) air of fan conveying flows through air passage, will be taken away in the heat of the hot linked radiator in the air passage.
Compared with prior art, the device have the advantages that being：
Mixing, constant temperature, detection between sample and reagent are completed in detection module, simple in structure, compact；And it can set
Multiple sense channels are put, considerably improve detection efficiency；
High degree of automation, using the mobile sample-adding module of three-dimensional, automatically accurate quantification extracts sample, reagent and injects
In disc, the consumption of sample, reagent is reduced, and can automatically clean sample-adding module, is automatically completed correction；
The reagent holding time is long, goes to control the temperature of the reagent using the mode that refrigeration is strengthened radiating so that low temperature shape
Agent effective under state is more preferable.
Description of the drawings
Referring to the drawings, the disclosure will be easier to understand.Skilled addressee readily understands that be：This
A little attached drawings are used only for the technical solution illustrated the present invention, and are not intended to and protection scope of the present invention is construed as limiting.
In figure：
Fig. 1 is the structure diagram of the full automatic blood-coagulation detection analysis instrument of the present invention；
Fig. 2 is the structure diagram of the working panel of the present invention；
Fig. 3 is another structure diagram of working panel in Fig. 2；
Fig. 4 is the structure diagram of the sample-adding module of the present invention；
Fig. 5 is another structure diagram of the sample-adding module of the present invention；
Fig. 6 is the structure diagram of the detection module of the present invention；
Fig. 7 is another structure diagram of detection module in Fig. 6.
Specific embodiment
Fig. 1-7 and following description describe the present invention optional embodiment to instruct how those skilled in the art implement
It is of the invention with reproducing.In order to instruct technical solution of the present invention, simplified or some conventional aspects be omitted.Those skilled in the art
It should be appreciated that modification or replacement from these embodiments will within the scope of the invention.Those skilled in the art should understand that
Following characteristics can combine to form multiple modifications of the present invention in various ways.As a result, the invention is not limited in it is following can
Embodiment is selected, and is only limited by the claims and their equivalents.
Embodiment 1：
Fig. 1 schematically illustrates the basic block diagram of full automatic blood-coagulation detection analysis instrument of the embodiment of the present invention, such as Fig. 1 institutes
Show, the full automatic blood-coagulation detection analysis instrument includes：
Babinet is provided with working panel, pre- thermal potential 7, touch-screen 9 on the babinet；The pre- thermal potential 7 is arranged on the work
Make on the babinet above panel, for preheating micro-fluidic disc；
Fig. 2-3 schematically illustrates the basic block diagram of the working panel of the embodiment of the present invention, as Figure 2-3, institute
Stating working panel includes：
Cleaning position 1, sample position 2, Quality Control grade 3, reagent position 4 are set gradually from left to right；The cleaning position includes front and rear
Chamber 12, the service sink 11 of cleaning solution are strengthened in the receiving being arranged in order；The sample position 2 can accommodate multiple samples, it is front and rear successively
Arrangement, for a row, two row or multiple row；Quality Control grade accommodates a variety of quality-control products, front and rear to be arranged in order；Mark is set gradually from top to bottom
Know 21 (for indicating different position), circuit board 22 and stent 23；Wherein still further comprised in reagent position：
Refrigerating chamber 41, it is front and rear to be arranged in order for accommodate plurality of reagents, for a row, two row or multiple row；
Refrigerator 42, such as TEC, the refrigerator is used for the reagent to freeze in the reagent chamber；
Radiator 45, such as has the radiator of multiple radiation tooths, and the radiator is thermally connected with the refrigerator；
Air passage 43, the air passage are thermally connected with the radiator；
Fan 44, the fan are arranged on the inlet in the air passage；
QR codes identification module 8, the QR codes identification module 8 are arranged on the lower part of the cleaning position, and the side in babinet
In portion；
Fig. 4-5 schematically illustrates the basic block diagram of the sample-adding module of the embodiment of the present invention, as illustrated in figures 4-5, institute
Stating sample-adding module includes：
X is fixed on to stent on the babinet to stent, the X, has X direction guiding rails 511, the first motor 512, X to biography
Send band 514 and first rotating wheel, the X is wound on to conveyer belt on first motor and the first rotating wheel；
Y-direction stent, the position of the Y-direction stent to stent, connect the X to conveyer belt 514 simultaneously higher or lower than the X
511 horizontally slip on the X direction guiding rails；There is Y-direction guide rail 521, the second motor 522, Y-direction conveyer belt on the Y-direction stent
524 and second rotating wheel 523, the Y-direction conveyer belt be wound on second motor and the second rotating wheel；
Z-direction stent, the Z-direction stent connect the Y-direction conveyer belt 524 and are slid back and forth in the Y-direction guide rail 521；
There is Z-direction guide rail 531, the 3rd motor 532,534 and the 3rd rotating wheel 533 of Z-direction conveyer belt, the Z-direction passes on the Z-direction stent
Band is sent to be wound on the 3rd motor and the 3rd rotating wheel；
Sample needle 51, the sample needle connect the Z-direction conveyer belt by connector and are slided up and down on Z-direction guide rail；
By X under, the drive of Y-direction, Z-direction conveyer belt, the sample needle realizes left and right, front and rear and move up and down, so as to cleaning plus
Sample pin, sample needle extract quantitative sample, reagent and are injected into micro-fluidic disc respectively；
Quantitative drawing mechanism, by the combination of peristaltic pump, pipeline, quantitative drawing mechanism is the state of the art,
This is repeated no more；
Fig. 6-7 schematically illustrates the basic block diagram of the detection module of the embodiment of the present invention, as shown in fig. 6-7, institute
Stating detection module includes：
Micro-fluidic disc 611, the micro-fluidic disc have inlet, reactive tank and test chamber；
Bracket 621, the micro-fluidic disc 611 are arranged on the bracket 621, and the both sides of the bracket 621, which have, leads
Rail 624；
Motor 631, such as servomotor, the motor is fixed on 621 downside of bracket, for the disc to be driven to revolve
Turn；
Light source, such as photodiode, tungsten lamp, the light source is used to send measurement light；The optical coupling that measures is at least two
A optical fiber；
At least two optical fiber 642, the light input end of at least two optical fiber connect an only light source, light output end
641 are curvedly separately fixed on the bracket on the downside of the disc；
Slip lid blocks the micro-fluidic disc when the slip lid moves；The slip lid includes：
Cover board 671, support plate 622, the both sides of the support plate 622 are equipped with and the 624 matched sliding block 623 of guide rail；
There is through hole on the cover board, convenient for after slip lid playbacks sample needle below the slip lid and blocked by slip lid micro-fluidic
Reagent, sample are added in disc；
Detector 661, the detector 661 and circuit 662 are arranged between cover board and support plate, for will be by the inspection
It surveys the measurement light after intracavitary sample absorbs and is converted to electric signal, and send the analysis module；
Heating member 651,652, the heating member are used to heat the micro-fluidic disc, and disc is made to maintain design temperature,
It is separately positioned on bracket and slip lid；Wherein heating member 651 is arranged on the downside of bracket 621, and heating member 652 is arranged on detector
Between support plate.
The full automatic blood-coagulation determination method of the embodiment of the present invention, the method for work comprise the following steps：
(A1) QR codes identification module identifies sample and stores, and sample, reagent are respectively put into sample position, reagent position；
(A2) push slip lid open, will be put into the preheated micro-fluidic disc of pre- thermal potential in detection module；
(A3) the slip lid return, the sample needle after service sink cleaning extracts quantitative sample, reagent respectively, and injects
Micro-fluidic disc；
(A4) the motor driving micro-fluidic disc rotation, sample and reagent mixing；
Heating member heats the micro-fluidic disc, and disc is made to maintain certain temperature；
(A5) the measurement light that light source is sent is transferred to by multiple beams of optical fiber below disc, through the mixture in test chamber,
The electric signal of multiple detector outputs send analysis module；
(A6) analysis module handles the electric signal, so as to know the parameter of sample.
Claims (6)
1. a kind of full automatic blood-coagulation detection analysis instrument, the full automatic blood-coagulation detection analysis instrument includes babinet, sample-adding module, cleaning
Module, detection module and analysis module；It is characterized in that：The detection module includes：
Micro-fluidic disc, the micro-fluidic disc have inlet, reactive tank and test chamber；
Motor, the motor are used to that the disc to be driven to rotate；
Light source, the light source are used to send measurement light；
Detector, the detector is used to the measurement light after being absorbed by sample in the test chamber being converted to electric signal, and send
The analysis module；
Heating member, the heating member are used to heat the micro-fluidic disc；
Bracket, the bracket are equipped with guide rail, and the micro-fluidic disc, motor are mounted on the bracket；
Slip lid, the slip lid are arranged on the guide rail, and the micro-fluidic disc is blocked when the slip lid moves；The slip lid
Including：
Cover board, support plate, the cover board are fixed in the support plate, and with through hole, suitable for the sample needle after slip lid playbacks
Reagent, sample are added in micro-fluidic disc that is below the slip lid and being blocked by slip lid；The both sides of the support plate are equipped with
With the sliding block of the guide rail；
The detector is arranged between cover board and support plate；
The babinet is provided with working panel, and sample position, reagent position, cleaning position are set on the working panel；
The sample-adding module includes three-dimensional moving arm, sample needle；
The reagent position includes：
Mark, the mark is arranged on the working panel, for labelled reagent；
Reagent chamber, the reagent chamber are used to accommodate reagent；
Refrigerator, the refrigerator are used for the reagent to freeze in the reagent chamber；
Radiator, the radiator are thermally connected with the refrigerator；
Air passage, the air passage are thermally connected with the radiator；
Fan, the fan are arranged on the inlet in the air passage；The mark, reagent chamber, refrigerator, radiator and air passage are certainly
It is set gradually under above.
2. full automatic blood-coagulation detection analysis instrument according to claim 1, it is characterised in that：The detection module further wraps
It includes：
At least two optical fiber, the light input end of at least two optical fiber connect an only light source, and light output end is arc-shaped
Ground is separately fixed at the upside or downside of the disc；
The quantity of the detector and the quantity Matching of the light output end.
3. full automatic blood-coagulation detection analysis instrument according to claim 2, it is characterised in that：At least two light output ends are fixed
On the bracket, the detector is fixed on the slip lid.
4. full automatic blood-coagulation detection analysis instrument according to claim 1, it is characterised in that：The sample-adding module includes：
For X to stent, the X has X direction guiding rails, the first motor, X to conveyer belt and the first rotating wheel on stent, and the X is to biography
Band is sent to be wound on first motor and the first rotating wheel；
Y-direction stent, the Y-direction stent connect the X and horizontally slip to conveyer belt and on the X direction guiding rails；The Y-direction stent
Upper have Y-direction guide rail, the second motor, Y-direction conveyer belt and the second rotating wheel, the Y-direction conveyer belt be wound on second motor and
On second rotating wheel；
Z-direction stent, the Z-direction stent connect the Y-direction conveyer belt and are slid back and forth in the Y-direction guide rail；The Z-direction stent
Upper have Z-direction guide rail, the 3rd motor, Z-direction conveyer belt and the 3rd rotating wheel, the Z-direction conveyer belt be wound on the 3rd motor and
On 3rd rotating wheel；
Sample needle, the sample needle connect the Z-direction conveyer belt by connector and are slided up and down on Z-direction guide rail.
5. full automatic blood-coagulation detection analysis instrument according to claim 1, it is characterised in that：The full automatic blood-coagulation detection point
Analyzer further comprises：
Pre- thermal potential, the pre- thermal potential are used to preheat the micro-fluidic disc.
6. according to the method for work of any full automatic blood-coagulation detection analysis instruments of claim 1-5, the method for work bag
Include following steps：
（A1）Sample, reagent are put into sample position, reagent position respectively；
（A2）It pushes slip lid open, preheated micro-fluidic disc is put into detection module；
（A3）Sample-adding module after cleaning extracts quantitative sample, reagent respectively, and injects micro-fluidic disc, and the slip lid returns
Position；
（A4）The motor driving micro-fluidic disc rotation, sample and reagent mixing；
Heating member heats the micro-fluidic disc；
（A5）The measurement light that light source is sent send analysis module through the mixture in test chamber, the electric signal of detector output；
（A6）The analysis module processing electric signal, so as to know the parameter of sample；
The method of work is further comprising the steps of：
（B1）Reagent in refrigerator refrigeration reagent chamber；
（B2）The heat of the refrigerator generation is distributed with the hot linked radiator of the refrigerator；
（B3）The air of fan conveying flows through air passage, will be taken away with the heat of the hot linked radiator in the air passage.
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| CN201610268795.3A CN105759066B (en) | 2016-04-26 | 2016-04-26 | Full automatic blood-coagulation detection analysis instrument, method |
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| CN201610268795.3A CN105759066B (en) | 2016-04-26 | 2016-04-26 | Full automatic blood-coagulation detection analysis instrument, method |
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| CN205620422U (en) * | 2016-04-26 | 2016-10-05 | 绍兴普施康生物科技有限公司 | Full -automatic blood coagulation detecting analyzer |
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