CN109142705B - Biochemical immunity analyzer and working method thereof - Google Patents
Biochemical immunity analyzer and working method thereof Download PDFInfo
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- CN109142705B CN109142705B CN201810782894.2A CN201810782894A CN109142705B CN 109142705 B CN109142705 B CN 109142705B CN 201810782894 A CN201810782894 A CN 201810782894A CN 109142705 B CN109142705 B CN 109142705B
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000036039 immunity Effects 0.000 title claims abstract description 17
- 238000001514 detection method Methods 0.000 claims abstract description 43
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 238000005259 measurement Methods 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 239000003085 diluting agent Substances 0.000 claims description 35
- 238000002156 mixing Methods 0.000 claims description 25
- 238000003860 storage Methods 0.000 claims description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims description 17
- 238000009826 distribution Methods 0.000 claims description 8
- 238000003018 immunoassay Methods 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 230000000903 blocking effect Effects 0.000 claims description 6
- 230000001133 acceleration Effects 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 2
- 230000001678 irradiating effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000008280 blood Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 238000004879 turbidimetry Methods 0.000 description 4
- 238000005452 bending Methods 0.000 description 3
- 238000004737 colorimetric analysis Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004848 nephelometry Methods 0.000 description 1
- 230000036316 preload Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention provides a biochemical immunity analyzer and a working method thereof, wherein the biochemical immunity analyzer comprises a disc; the cavity is provided with an opening, and a guide rail is arranged in the cavity; the bracket is provided with a rotating shaft, a tray, a bracket and a shielding piece, the bracket is arranged on the guide rail, the rotating shaft is arranged on the bracket, the tray is fixed on the rotating shaft, the rotating shaft penetrates through the through hole of the disc, the tray bears the disc, and the shielding piece is arranged on the bracket and is used for sealing the opening when the bracket enters the cavity; the first driving unit is used for driving the bracket to move on the guide rail; the second driving unit is used for driving the rotating shaft to rotate; the first measuring light emitted by the first light source is suitable for passing through the detection cavity on the disc; the second measuring light emitted by the second light source is suitable for irradiating the detection cavity on the disc; the first detector is used for receiving first measuring light after passing through the detection cavity; the second detector receives scattered light of the second measurement light on the liquid in the detection chamber. The invention has the advantages of simple structure, complete functions, convenient operation and the like.
Description
Technical Field
The invention relates to biochemical immunity analysis, in particular to a biochemical immunity analyzer based on a plurality of detection positions and a working method thereof.
Background
The traditional biochemical analyzer adopts a colorimetry and a turbidimetry to analyze the substance content of the blood sample, the colorimetry can detect most blood biochemical test items, and the turbidimetry can detect part of blood immunological test items. The test method is that the serum sample and the reagent are added into a cuvette for reaction at 37 ℃ and detection, the reacted liquid has absorption and scattering effects on monochromatic light with different wavelengths, the transmitted light is weakened, and the content of the detection substance can be obtained through the weakened degree.
The traditional specific protein analyzer mostly adopts a scattering turbidimetry mode to detect, a sample and a reaction reagent are added into a cuvette, the antigen suspended in the liquid is detected to react with the corresponding antibody, and immune complex particles are formed on the premise of excessive antibodies. Scattered light generated by the light beam emitted by the light source passing through the suspended particles is received by the detector.
At present, a full-automatic biochemical analyzer and a specific protein analyzer are commonly used in large hospitals, and the full-automatic biochemical analyzer and the specific protein analyzer both comprise large-size mechanical sample adding arms, are complex in structure, high in price and large in sample consumption, and are not suitable for basic medical institutions because of reagent calibration by professionals.
The semi-automatic biochemical analyzer and the specific protein analyzer are usually used in hospitals below the second level, but the semi-automatic biochemical analyzer and the specific protein analyzer are required to be manually participated, the non-human interference is difficult to ensure, and the user is required to finish the very specialized operations such as reagent calibration and the like, so that the operation is troublesome.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides the biochemical immunity analyzer which has the advantages of simple structure, complete functions, small sample consumption, convenient operation, portability and suitability for being used together by various detection methods.
The invention aims at realizing the following technical scheme:
A biochemical immunoassay analyzer, the biochemical immunoassay analyzer comprising a disc; the biochemical immunity analyzer further comprises:
The cavity is provided with an opening, and a guide rail is arranged in the cavity;
The bracket is provided with a rotating shaft, a tray, a bracket and a shielding piece, the bracket is arranged on the guide rail, the rotating shaft is arranged on the bracket, the tray is fixed on the rotating shaft, the rotating shaft penetrates through the through hole of the disc, the tray bears the disc, and the shielding piece is arranged on the bracket and is used for sealing the opening when the bracket enters the cavity;
the first driving unit is used for driving the bracket to move on the guide rail;
A second driving unit for driving the rotation shaft to rotate;
A first light source adapted to emit a first measurement light through a detection cavity on the disc;
a second light source adapted to illuminate a detection cavity on the disc with a second measurement light emitted by the second light source;
A first detector for receiving a first measurement light after passing through the detection cavity;
and the second detector is used for receiving scattered light of second measuring light on the liquid in the detection cavity.
The invention also aims to provide a working method based on the biochemical immunity analyzer, and the invention aims to be realized through the following technical scheme:
The working method of the biochemical immunity analyzer comprises the following steps:
(A1) The disc with the added sample is placed on the rotating shaft and is carried by the tray;
(A2) Under the action of a first driving unit, the disc moves on the guide rail along with the bracket and enters the cavity, and the shielding piece seals the outlet of the cavity;
(A3) Under the action of a second driving unit, the disc rotates at different rotating speeds, so that a sample in the disc and a pre-filled diluent in the disc are mixed, and then react with the pre-filled reagent in the detection cavity;
(A4) The first measuring light and the second measuring light irradiate the liquid in the detection cavity and are received; the parameters of the sample are known by analyzing the output signals of the first detector and the second detector.
Compared with the prior art, the invention has the following beneficial effects:
1. the biochemical immunity analyzer can realize the functions of two devices (a biochemical analyzer and a specific protein analyzer) at the same time, and can realize the on-machine detection of whole blood;
2. the analyzer of the invention has small volume and portability, and is particularly suitable for private doctors to go to the gate for service;
3. the invention pre-loads the diluent and the freeze-dried reagent in the disc, an operator only needs to add about 0.1 milliliter of trace sample, other samples are all automatically carried out, the artificial interference is eliminated, the operation is simple, the sample usage amount is small, and the invention is a typical point of care testing (POCT) product and is suitable for basic medical institutions;
4. The disc realizes sample separation, mixing, reaction and multi-method detection, has the advantages of simple structure, comprehensive functions, convenient operation and less human participation, and does not need the intervention of professional operators;
5. The reaction reagent is pre-loaded in the disc in a freeze-dried mode, so that a user does not need to perform reagent calibration operation, the specialization is realized, and the method is suitable for detecting basic medical institutions with low specialization degree;
6. the disc is used as a low-cost disposable consumable, diluent and reagent are preloaded inside, and the whole operation process is free from leakage, so that the infection risk of operators is greatly reduced, and medical wastewater discharge is avoided, and the device is safe and environment-friendly.
Drawings
The present disclosure will become more readily understood with reference to the accompanying drawings. As will be readily appreciated by those skilled in the art: the drawings are only for illustrating the technical scheme of the present invention and are not intended to limit the scope of the present invention. In the figure:
FIG. 1 is a schematic diagram of a biochemical immunoassay device according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a disc according to an embodiment of the present invention;
FIG. 3 is a schematic view of a rotating shaft and a tray according to an embodiment of the present invention;
FIG. 4 is a schematic view of a rotating shaft according to an embodiment of the present invention;
fig. 5 is a schematic structural view of a tray according to an embodiment of the present invention.
Detailed Description
Figures 1-5 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and reproduce the invention. In order to teach the technical solution of the present invention, some conventional aspects have been simplified or omitted. Those skilled in the art will appreciate variations or alternatives derived from these embodiments that fall within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Thus, the invention is not limited to the following alternative embodiments, but only by the claims and their equivalents.
Example 1:
Fig. 1 schematically shows a schematic structure of a biochemical immunoassay analyzer according to an embodiment of the present invention, as shown in fig. 1, comprising:
a disc, fig. 2 schematically shows a schematic structure of a disc according to an embodiment of the present invention, as shown in fig. 2, the disc includes:
a body 1 having a fixing portion 2 adapted to be connected to a rotating shaft of a motor or the like for rotation under external power drive of the motor or the like; the body is formed with:
a diluent chamber 3, the diluent chamber 3 being disposed around an outer edge of the fixed portion 2; the diluent cavity 3 is preloaded with diluent and is provided with an outlet 7; in order to prevent leakage when not in use, the outlet can be additionally provided with a sealing film which is torn off in the diluent for use;
the first quantitative cavity 6 is arranged at the periphery of the diluent cavity 3 and is communicated with the diluent cavity 3; the first overflow port 15 is formed at one end of the first metering cavity 6, which is close to the center of the body; the first metering chamber 6 is communicated with the mixing chamber 8 through a first channel 12;
a sample chamber 10, wherein the sample chamber 10 is arranged at the periphery of the diluent chamber 3;
A second quantitative chamber 4, the second quantitative chamber 4 being disposed at the periphery of the sample chamber 10 and communicating with the sample chamber 10; the end of the second quantifying cavity 4, which is close to the center of the body, is provided with a second overflow port 14, and the second quantifying cavity 4 is communicated with the mixing cavity 8 through a second channel 11;
A first storage chamber 5, wherein the first storage chamber 5 is arranged at the periphery of the second quantifying chamber 4 and is communicated with the second quantifying chamber 4;
the second storage cavity is communicated with the second overflow port;
the third storage cavity is communicated with the first overflow port;
A first channel 12, the first channel 12 having a flow blocking structure, such as a microfluidic valve provided on the channel, or a capillary tube having a bent structure;
A second channel 11, the second channel 11 having a flow blocking structure, such as a microfluidic valve provided on the channel, or a capillary tube having a bent structure;
A mixing chamber 8, wherein the mixing chamber 8 is arranged at the periphery of the first quantifying chamber 6 and is communicated with a distributing chamber through a third channel 13;
A third channel 13, the third channel 13 having a choke structure, such as a microfluidic valve provided on the channel, or a capillary tube having a bent structure;
A distribution chamber which is arranged at the periphery of the mixing chamber 8 and is communicated with a plurality of detection chambers 9 through channels;
A plurality of detection chambers 9 disposed at the periphery of the distribution chamber;
A cavity 81 having an opening, a guide rail being provided inside the cavity, and linear guide rails being provided on both left and right sides of the cavity as described below;
A bracket 82 having a rotation shaft, a tray, a support, and a shutter, the support being provided on the guide rail, the rotation shaft being provided on the support, the tray being fixed on the rotation shaft, the rotation shaft passing through the through hole of the disk, the tray carrying the disk, the shutter being provided on the support for closing the opening when the bracket enters the cavity;
the first driving unit is used for driving the bracket to move on the guide rail;
A second driving unit for driving the rotation shaft to rotate;
A first light source 83 adapted to emit a first measuring light through a detection cavity on the disc; based on the above, the first light source and the first detector are arranged on the cavity and are respectively positioned on the upper side and the lower side of the disc in the cavity;
A second light source 85 adapted to illuminate a detection cavity on the disc with a second measurement light emitted by the second light source; based on this, the second light source and the second detector are disposed on a sidewall of the cavity;
a first detector 84 for receiving a first measurement light after passing through the detection cavity;
a second detector 86 which receives scattered light of the second measuring light on the liquid in the detection chamber.
In order to enhance the fixing reliability between the disc and the rotating shaft and prevent the disc from shaking on the rotating shaft, further, the rotating shaft is a flower-shaped shaft, and the inner wall of the through hole is matched with the outer edge of the rotating shaft.
In order to enhance the reliability of the fixation between the tray and the rotating shaft and prevent the tray from shaking on the rotating shaft, further, as shown in fig. 3-5, the tray 23 is sleeved on the rotating shaft 22, and the tray 23 is provided with a through hole matched with the outer edge of the rotating shaft 22; the tray is integrated with the rotating shaft or is arranged independently.
In order to prevent the disk from being thrown out during rotation, further, as shown in fig. 3 to 5, the biochemical immunoassay analyzer further comprises:
an elastic member 25 provided inside the rotation shaft;
The movable member 26, the part of the movable member connected with the elastic member is located outside the outer edge of the rotating shaft, and the exposed part has an arc-shaped surface.
In order to improve manufacturing efficiency of the disk, the body includes:
A first portion, the first portion and the second portion being disposed one above the other; the first part is provided with a through hole, and the position of the through hole corresponds to the position of the sample cavity of the second part, so that a sample is added into the sample cavity;
And a second part, wherein the diluent cavity, the sample cavity, the first quantifying cavity, the second quantifying cavity, the mixing cavity, the first storage cavity, the second storage cavity, the third storage cavity, the first channel, the second channel, the third channel, the distribution cavity and the detection cavity are formed on the second part, such as a groove is engraved on the second part, and then are in sealing connection with the first part, so that each cavity and each channel are formed.
The working method of the biochemical immunity analyzer provided by the embodiment of the invention comprises the following steps:
(A1) The disc with the added sample is placed on the rotating shaft and is carried by the tray; the disc is placed in the following manner:
(B1) Placing a disc, wherein a through hole of the disc faces the rotating shaft;
(B2) Pressing the disk downward, wherein a part of the movable piece in the rotating shaft protruding out of the outer edge of the rotating shaft is extruded by the inner wall of the through hole of the disk and retreats towards the inside of the rotating shaft;
When the disc moves downwards below the movable piece, the movable piece rebounds under the action of the elastic piece, and part of the movable piece protrudes out of the outer edge of the rotating shaft;
(B3) The disc moves downwards along the rotating shaft, and the inner wall of the through hole of the disc is matched with the outer wall of the rotating shaft;
(A2) Under the action of a first driving unit, the disc moves on the guide rail along with the bracket and enters the cavity, and the shielding piece seals the outlet of the cavity;
(A3) Under the action of a second driving unit, the disc rotates at different rotating speeds, so that a sample in the disc and a pre-filled diluent in the disc are mixed, and then react with the pre-filled reagent in the detection cavity; the specific method is as follows:
(C1) The disc rotates at a first rotation speed, a sample in the sample adding cavity enters the second quantifying cavity and the first storing cavity, substances with higher density in the sample enter the first storing cavity, and liquid with lower density enters the second quantifying cavity; the redundant sample flows out of the second overflow port and enters the second storage cavity; the volume of the first storage cavity is larger than the volume of the substance with higher density in the sample;
Meanwhile, pre-filled diluent in the diluent cavity is thrown out from the outlet and enters the first quantitative cavity, and the redundant diluent flows out through the first overflow port and enters the third storage cavity; if the sealing film is arranged at the outlet, the sealing film needs to be torn off;
(C2) Stopping the rotation of the disc, wherein the first channel and the second channel are filled with liquid;
(C3) The disc rotates at a second rotating speed, the diluent in the first quantitative cavity enters the mixing cavity through the first channel, and the liquid with smaller density in the second quantitative cavity enters the mixing cavity;
(C4) The disc enters an acceleration and deceleration circulation stage, and liquid with smaller density in the sample and diluent are mixed in a mixing cavity through multiple high-speed and low-speed conversion;
(C5) Stopping the rotation of the disc, wherein the third channel is filled with liquid;
(C6) The disc rotates at a third rotating speed, liquid in the mixing cavity enters the distribution cavity through a third channel and then enters each detection cavity, and the liquid dissolves the pre-filled reagent in the detection cavity, such as the reagent after freeze drying;
(C7) The disc rotates at a fourth rotating speed, the fourth rotating speed is smaller than the first rotating speed, the second rotating speed and the third rotating speed, and the liquid in the plurality of detection cavities reacts in a constant temperature environment;
(A4) The first measuring light and the second measuring light irradiate the liquid in the detection cavity and are received; the parameters of the sample are known by analyzing the output signals of the first detector and the second detector, such as by colorimetry, turbidimetry and nephelometry.
Example 2:
the biochemical immunity analyzer and the working method thereof according to the embodiment 1 of the invention are applied to biochemical projects.
In this application example, as shown in fig. 1 to 5, the body is made of resin plastic, and is composed of a first part and a second part which are arranged up and down, and the quantitative cavity, the mixing cavity, the detection cavity and the like are all formed on the second part through grooving; the diluent and the reagent are preloaded in the disc; the first channel adopts a capillary tube with a bending structure, and the minimum distance from the rotation center of the body to the first channel is smaller than the minimum distance from the rotation center of the body to the first metering cavity; the second channel adopts a capillary tube with a bending structure, and the minimum distance from the rotation center of the body to the second channel is smaller than the minimum distance from the rotation center of the body to the second quantifying cavity; the third channel adopts a capillary tube with a bending structure, and the minimum distance from the rotation center of the body to the third channel is smaller than the minimum distance from the rotation center of the body to the first mixing cavity; the outer edge of the rotating shaft 22 is provided with arc-shaped bulges 27 which are uniformly and alternately distributed and extend along the direction parallel to the axis of the rotating shaft, such as 5 bulges, and the upper end and the lower end of the bulges are provided with chamfers 28, so that the disc can be conveniently clamped in smoothly; corresponding to the protrusions 27, the inner wall of the through hole of the body is provided with evenly distributed grooves matched with the protrusions; 2 movable pieces 26 are spherical, the rotating shaft 22 is provided with a through hole 29, and the movable pieces are propped against the through hole of the rotating shaft; the diameter of the through hole is smaller than that of the elastic piece; the elastic piece adopts a spring; the projection of the movable piece on a plane perpendicular to the central axis of the rotating shaft is positioned between the projections of adjacent bulges on the plane; the inner wall of the through hole of the tray 23 is provided with a groove 31 matched with the bulge 27 at the outer edge of the rotating shaft 22, so that the tray 23 is clamped on the rotating shaft 22; the screw 24 sequentially passes through the through hole 32 of the tray 23 and the through hole 20 of the rotating shaft and abuts against the rotating shaft of the motor 21, thereby firmly fixing the rotating shaft 22 and the tray 23 to the motor rotating shaft.
According to the working method of the biochemical immunity analyzer, the step (A3) of the working method specifically comprises the following steps:
(C1) The fixed part is connected and fixed with the motor rotating shaft, and a trace amount of sample to be tested, such as 0.1ml of whole blood, is added into the sample adding cavity; tearing off the sealing film;
The disc rotates at a first rotation speed, a sample in the sample adding cavity enters the second quantifying cavity and the first storing cavity, substances with higher density in the sample enter the first storing cavity, and liquid with lower density enters the second quantifying cavity; the redundant sample flows out of the second overflow port and enters the second storage cavity; the volume of the first storage cavity is larger than the volume of the substance with higher density in the sample;
Meanwhile, pre-filled diluent in the diluent cavity is thrown out from the outlet and enters the first quantitative cavity, and the redundant diluent flows out through the first overflow port and enters the third storage cavity;
(C2) The disc stops rotating, and the first channel and the second channel are filled with liquid due to capillary action;
(C3) The disc rotates at a second rotating speed, the diluent in the first quantitative cavity enters the mixing cavity through the first channel, and the liquid with smaller density in the second quantitative cavity enters the mixing cavity;
(C4) The disc enters an acceleration and deceleration circulation stage, and liquid with smaller density in the sample and diluent are mixed in a mixing cavity through multiple high-speed and low-speed conversion;
(A5) The disc stops rotating, and the third channel is filled with liquid due to capillary action;
(C6) The disc rotates at a third rotating speed, liquid in the mixing cavity enters the distribution cavity through a third channel due to siphoning effect and then enters each detection cavity of one circle, and the liquid dissolves the pre-filled reagent in the detection cavity, such as the reagent after freeze drying;
(C7) The disc rotates at a fourth rotating speed, the fourth rotating speed is smaller than the first rotating speed, the second rotating speed and the third rotating speed, and the liquid in the plurality of detection cavities reacts in a constant temperature environment.
Example 3:
the biochemical immunity analyzer and the working method thereof according to the embodiment 1 of the invention are applied to the immunity project.
Unlike example 2, the following is: the flow blocking structure adopts a micro-flow valve, and when the rotating speed reaches a certain value, the liquid breaks through the micro-flow valve and enters the next cavity.
Claims (4)
1. A biochemical immunoassay analyzer, the biochemical immunoassay analyzer comprising a disc; the method is characterized in that: the biochemical immunity analyzer further comprises:
The cavity is provided with an opening, and a guide rail is arranged in the cavity;
The bracket is provided with a rotating shaft, a tray, a bracket and a shielding piece, the bracket is arranged on the guide rail, the rotating shaft is arranged on the bracket, the tray is fixed on the rotating shaft, the rotating shaft penetrates through the through hole of the disc, the tray bears the disc, and the shielding piece is arranged on the bracket and is used for sealing the opening when the bracket enters the cavity;
the first driving unit is used for driving the bracket to move on the guide rail;
A second driving unit for driving the rotation shaft to rotate;
A first light source adapted to emit a first measurement light through a detection cavity on the disc;
a second light source adapted to illuminate a detection cavity on the disc with a second measurement light emitted by the second light source;
a first detector for receiving a first measurement light after passing through the detection cavity; the first light source and the first detector are arranged on the cavity and are respectively positioned on the upper side and the lower side of the disc in the cavity; the second light source and the second detector are arranged on the side wall of the cavity;
A second detector that receives scattered light of a second measurement light on the liquid in the detection cavity;
The rotating shaft is a flower-shaped shaft, and the inner wall of the through hole is matched with the outer edge of the rotating shaft; the outer edge of the rotating shaft is provided with arc-shaped bulges which are distributed at intervals and extend along the direction parallel to the axis of the rotating shaft;
an elastic member disposed inside the rotation shaft;
the part of the movable piece connected with the elastic piece is positioned outside the outer edge of the rotating shaft, the exposed part is provided with an arc-shaped surface, and the projection of the movable piece on a plane perpendicular to the central axis of the rotating shaft is positioned between the projections of adjacent bulges on the plane; the movable piece is spherical, the rotating shaft is provided with another through hole, and the movable piece is propped against the through hole of the rotating shaft; the diameter of the through hole of the rotating shaft is smaller than that of the elastic piece;
The disk includes:
The body is provided with a through hole;
the diluent cavity is arranged around the outer edge of the through hole of the body; the diluent chamber has an outlet;
The first quantitative cavity is arranged at the periphery of the diluent cavity and is communicated with the diluent cavity; one end of the first quantifying cavity, which is close to the center of the body, is provided with a first overflow port; the first quantitative cavity is communicated with the mixing cavity through a first channel;
The sample cavity is arranged at the periphery of the diluent cavity;
the second quantitative cavity is arranged at the periphery of the sample cavity and is communicated with the sample cavity; one end of the second quantifying cavity, which is close to the center of the body, is provided with a second overflow port, and the second quantifying cavity is communicated with the mixing cavity through a second channel;
The first storage cavity is arranged at the periphery of the second quantifying cavity and is communicated with the second quantifying cavity;
the second storage cavity is communicated with the second overflow port;
the third storage cavity is communicated with the first overflow port;
a first passage having a flow blocking structure;
a second channel having a flow blocking structure;
The mixing cavity is arranged at the periphery of the first quantifying cavity and the second quantifying cavity and is communicated with the distributing cavity through a third channel;
a third passage having a flow blocking structure;
the distribution cavity is arranged at the periphery of the mixing cavity and is communicated with the plurality of detection cavities through channels;
The detection cavities are arranged at the periphery of the distribution cavity.
2. The method of operating a biochemical immunoassay according to claim 1, comprising the steps of:
(A1) The disc with the added sample is placed on the rotating shaft and is carried by the tray;
(A2) Under the action of a first driving unit, the disc moves on the guide rail along with the bracket and enters the cavity, and the shielding piece seals the outlet of the cavity;
(A3) Under the action of a second driving unit, the disc rotates at different rotating speeds, so that a sample in the disc and a pre-filled diluent in the disc are mixed, and then react with the pre-filled reagent in the detection cavity;
(A4) The first measuring light and the second measuring light irradiate the liquid in the detection cavity and are received; the parameters of the sample are known by analyzing the output signals of the first detector and the second detector.
3. The method of operation of claim 2, wherein: in the step (A1), the disc is placed in the following manner:
(B1) Placing a disc;
(B2) Pressing the disk downward, wherein a part of the movable piece in the rotating shaft protruding out of the outer edge of the rotating shaft is extruded by the inner wall of the through hole of the disk and retreats towards the inside of the rotating shaft;
When the disc moves downwards below the movable piece, the movable piece rebounds under the action of the elastic piece, and part of the movable piece protrudes out of the outer edge of the rotating shaft;
(B3) The disc moves downwards along the rotating shaft, and the inner wall of the through hole of the disc is matched with the outer wall of the rotating shaft.
4. The method of operation of claim 2, wherein: step (A3) further comprises the steps of:
(C1) The disc rotates at a first rotation speed, a sample in the sample adding cavity enters the second quantifying cavity and the first storing cavity, substances with higher density in the sample enter the first storing cavity, and liquid with lower density enters the second quantifying cavity; the redundant sample flows out of the second overflow port and enters the second storage cavity;
Meanwhile, pre-filled diluent in the diluent cavity is thrown out from the outlet and enters the first quantitative cavity, and the redundant diluent flows out through the first overflow port and enters the third storage cavity;
(C2) Stopping the rotation of the disc, wherein the first channel and the second channel are filled with liquid;
(C3) The disc rotates at a second rotating speed, the diluent in the first quantitative cavity enters the mixing cavity through the first channel, and the liquid with smaller density in the second quantitative cavity enters the mixing cavity;
(C4) The disc enters an acceleration and deceleration circulation stage, and liquid with smaller density in the sample and diluent are mixed in a mixing cavity through multiple high-speed and low-speed conversion;
(C5) Stopping the rotation of the disc, wherein the third channel is filled with liquid;
(C6) The disc rotates at a third rotating speed, liquid in the mixing cavity enters the distribution cavity through a third channel and then enters each detection cavity, and the liquid dissolves the pre-filled reagent in the detection cavity;
(C7) The disc rotates at a fourth rotating speed, the fourth rotating speed is smaller than the first rotating speed, the second rotating speed and the third rotating speed, and the liquid in the detection cavity reacts in a constant temperature environment.
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| CN110308096A (en) * | 2019-04-09 | 2019-10-08 | 宁波普芯生物科技有限公司 | Combined centrifugal disc, analytical equipment and method |
| CN110286119B (en) * | 2019-06-28 | 2024-04-19 | 武汉明德生物科技股份有限公司 | TIP head detection frame, sample type detection module assembly and chemiluminescence immunoassay analyzer |
| CN111474151B (en) * | 2020-04-18 | 2023-03-21 | 杭州准芯生物技术有限公司 | Liquid detection method |
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