CN108646015A - Integrated apparatus based on multiple check bits and analysis method - Google Patents
Integrated apparatus based on multiple check bits and analysis method Download PDFInfo
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- CN108646015A CN108646015A CN201810782047.6A CN201810782047A CN108646015A CN 108646015 A CN108646015 A CN 108646015A CN 201810782047 A CN201810782047 A CN 201810782047A CN 108646015 A CN108646015 A CN 108646015A
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- 238000004458 analytical method Methods 0.000 title claims abstract description 26
- 238000003860 storage Methods 0.000 claims abstract description 50
- 238000010790 dilution Methods 0.000 claims abstract description 47
- 239000012895 dilution Substances 0.000 claims abstract description 47
- 238000002156 mixing Methods 0.000 claims abstract description 38
- 238000012360 testing method Methods 0.000 claims abstract description 32
- 238000009826 distribution Methods 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims description 34
- 230000036039 immunity Effects 0.000 claims description 20
- 239000003153 chemical reaction reagent Substances 0.000 claims description 16
- 238000005452 bending Methods 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 8
- 238000004737 colorimetric analysis Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 230000001133 acceleration Effects 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 2
- 230000003287 optical effect Effects 0.000 claims description 2
- 230000011664 signaling Effects 0.000 claims description 2
- 238000002834 transmittance Methods 0.000 claims 1
- 238000007865 diluting Methods 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 7
- 230000010354 integration Effects 0.000 description 7
- 238000005259 measurement Methods 0.000 description 5
- 238000004879 turbidimetry Methods 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 239000011246 composite particle Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002906 medical waste Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 238000012123 point-of-care testing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
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- Food Science & Technology (AREA)
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- Physics & Mathematics (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The present invention provides integrated apparatus and analysis method based on multiple check bits, integrated apparatus includes ontology；The outer rim for diluting sap cavity around the fixed part is arranged；First quantitative chamber is arranged in the periphery of dilution sap cavity；Sample cavity is arranged in the periphery of dilution sap cavity；Second quantitative chamber is arranged in the periphery of sample cavity, and is connected to；First storage chamber is arranged in the periphery of the second quantitative chamber, and is connected to the described second quantitative chamber；Second storage chamber is connected to second overflow port；Third storage chamber is connected to first overflow port；First is respectively provided with reducing-flow structure to third channel；Mixing chamber is arranged in the periphery of the first quantitative chamber, and is connected to distribution cavity by third channel；Distribution cavity is arranged in the periphery of the mixing chamber, and is connected to multiple test chambers by channel；Multiple test chambers are arranged in the periphery of the distribution cavity.The present invention has many advantages, such as that simple in structure, function is complete, easy to operate.
Description
Technical field
The present invention relates to biochemical analysis, more particularly to a kind of integrated apparatus and analysis method based on multiple check bits.
Background technology
Traditional Biochemical Analyzer analyzes the content of material of blood sample, colorimetric method using colorimetric method and turbidimetry
Most of blood biochemical, which can be detected, examines project, turbidimetry energy detection part haematogenic immunity to examine project.The method of inspection
It is that serum sample and reagent are added in cuvette to react and detect at 37 DEG C, list of the liquid after reaction to different wave length
Coloured light generates absorption and dispersion effect, transmitted light die down, and the content of detection substance can be obtained by the degree to die down.
The mode that traditional specific protein analyzer mostly uses scattering turbidimetry is detected, in cuvette be added sample and
Reaction reagent detects the antigen being suspended in liquid and reacts with corresponding antibodies, under the premise of antibody excess, is formed immune
Composite particles.The light beam that light source is sent out is received by scattering light caused by suspended particulate by detector.
Currently, large hospital is usually using automatic clinical chemistry analyzer and specific protein analyzer, include volume Pang
Big mechanical sample arm, complicated, expensive, sample consumption is big, and professional is needed to carry out reagent calibration, no
It is suitble to basic medical unit to use.
Hospital below two level needs too many ginseng manually usually using semi-automatic biochemical analyzer and specific protein analyzer
With, it is difficult to ensure that not by human interference, and user is needed to complete the very professional operations such as reagent calibration, it is troublesome in poeration.
Invention content
In order to solve the deficiency in above-mentioned prior art, the present invention provides a kind of simple in structure, functions entirely, sample
Small, easy to operate, suitable for a variety of detection methods the integrated apparatus based on multiple check bits of consumption.
The purpose of the present invention is what is be achieved through the following technical solutions：
A kind of integrated apparatus based on multiple check bits, the integrated apparatus include ontology, and the ontology has suitable
In being connected with the rotating shaft fixed part；The integrated apparatus further comprises being formed on the body：
Sap cavity is diluted, the dilution sap cavity is arranged around the outer rim of the fixed part；The dilution sap cavity has outlet；
First quantitative chamber, the described first quantitative chamber are arranged in the periphery of the dilution sap cavity, and connect with the dilution sap cavity
It is logical；One end for facing body central of described first quantitative chamber has the first overflow port；Described first quantitative chamber is logical by first
Road is connected to mixing chamber；
Sample cavity, the sample cavity are arranged in the periphery of the dilution sap cavity；
Second quantitative chamber, the described second quantitative chamber is arranged in the periphery of the sample cavity, and is connected to the sample cavity；Institute
The one end for facing body central for stating the second quantitative chamber has the second overflow port, and the described second quantitative chamber is by second channel and mixes
Close chamber connection；
First storage chamber, first storage chamber are arranged in the periphery of the described second quantitative chamber, and are quantified with described second
Chamber is connected to；
Second storage chamber, second storage chamber are connected to second overflow port；
Third storage chamber, the third storage chamber are connected to first overflow port；
First passage, the first passage have reducing-flow structure；
Second channel, the second channel have reducing-flow structure；
Mixing chamber, the mixing chamber is arranged in the periphery of the described first quantitative chamber, and is connected to distribution cavity by third channel；
Third channel, the third channel have reducing-flow structure；
Distribution cavity, the distribution cavity are arranged in the periphery of the mixing chamber, and are connected to multiple test chambers by channel；
Multiple test chambers, the test chamber are arranged in the periphery of the distribution cavity.
According to above-mentioned integrated apparatus, optionally, the ontology includes：
First part, the first part and second part are setting up and down；
Second part, the dilution sap cavity, sample cavity, the first quantitative chamber, the second quantitative chamber, mixing chamber, the first storage chamber,
Second storage chamber, third storage chamber, first passage, second channel, third channel, distribution cavity and test chamber are formed in described second
On part.
According to above-mentioned integrated apparatus, optionally, the outlet has sealer.
According to above-mentioned integrated apparatus, optionally, the dilution intracavitary pre-installs dilution.
According to above-mentioned integrated apparatus, it is preferable that the first passage uses the capillary with bending structure, described
The rotation center that the minimum range of the rotation center of ontology to the first passage is less than the ontology quantifies chamber to described first
Minimum range；Alternatively,
The second channel uses the capillary with bending structure, the rotation center of the ontology to the second channel
Minimum range be less than the ontology rotation center to described second quantitatively chamber minimum range；Alternatively,
The third channel uses the capillary with bending structure, the rotation center of the ontology to the third channel
Minimum range be less than the ontology rotation center to the mixing chamber minimum range.
According to above-mentioned integrated apparatus, it is preferable that reducing-flow structure uses microfluidic valve.
The present invention also aims to provide the biochemical immunity analyzer based on above-mentioned integrated apparatus, the goal of the invention
Technical scheme is that：
A kind of biochemical immunity analyzer, the biochemical immunity analyzer include light source, detector；The biochemical immunity analysis
Instrument further comprises：
Integrated apparatus, the integrated apparatus use above-mentioned integrated apparatus.
The present invention also aims to provide the analysis method based on above-mentioned biochemical immunity analyzer, which is
It is achieved by the following technical programs：
According to the analysis method of above-mentioned biochemical immunity analyzer, the analysis method includes the following steps：
(A1) it is fixed on the integrated apparatus in shaft to rotate with the first rotating speed, it is quantitative that the sample in feeding chamber enters second
In chamber and the first storage chamber, the larger substance of density enters in the first storage chamber in sample, and the smaller liquid of density enters second
Quantitative chamber；Extra sample is flowed out from the second overflow port, and is entered in the second storage chamber；
Meanwhile the dilution of dilution intracavitary prepackage is thrown away from the outlet, into the first quantitative intracavitary, extra is dilute
It releases liquid to flow out by the first overflow port, and enters in third storage chamber；
(A2) integrated apparatus stops rotating, hydraulically full in the first passage and second channel；
(A3) integrated apparatus is rotated with the second rotating speed, and the first quantitative intracavitary dilution enters mixing by first passage
Chamber, the liquid that the density of the described second quantitative intracavitary is smaller enter the mixing chamber；
(A4) integrated apparatus enters the cycle stage of acceleration and deceleration, is converted by multiple high speed low speed so that in sample
The smaller liquid of density and dilution mix in mixing chamber；
(A5) integrated apparatus stops rotating, hydraulically full in the third channel；
(A6) integrated apparatus is rotated with third rotating speed, and the liquid in mixing chamber enters by third channel in distribution cavity,
Enter in each test chamber later, liquid dissolves the reagent pre-installed in test chamber；
(A7) integrated apparatus is rotated with the 4th rotating speed, and the 4th rotating speed is less than the first rotating speed, the second rotating speed and third rotating speed,
Liquid in test chamber is reacted in isoperibol, and is detected by optical signalling.
According to above-mentioned analysis method, it is preferable that the volume of the first storage chamber is more than the body of the larger substance of density in sample
Product.
According to above-mentioned analysis method, optionally, in step (A2), the sealer of the opening is torn.
According to above-mentioned analysis method, it is preferable that in step (A7), utilize colorimetric method, turbidimetry and scattering ratio
Turbid method detection.
Compared with prior art, the device have the advantages that being：
1. the reagent after dilution and freeze-drying is mounted in integrated apparatus by the present invention in advance, operator only needs to add
0.1 milliliter or so of trace sample, other automations carry out, and eliminate human interference, easy to operate, sample usage amount
It is few, it is typical care diagnostic (POCT) product, is suitable for basic medical unit use；
2. the integrated apparatus of the present invention realizes sample separation, mixing, reaction and multi-method detection, simple in structure, work(
Can be comprehensively easy to operate, it is artificial to participate in less, being not necessarily to the intervention of professional operator；
3. reaction reagent is pre-installed in the form of freeze-drying in integrated apparatus, user is without carrying out reagent calibration behaviour
Make；
4. medical disposable material of the integrated apparatus as low cost, dilution and reagent are pre-installed in inside, whole operation
It does not leak outside in the process, greatly reduces the infection risk of operating personnel, and discharge without medical waste water, it is safe and environment-friendly；
5. the biochemical immunity analyzer of the present invention can be achieved at the same time two kinds of equipment (Biochemical Analyzer and specific protein analysis
Instrument) function, and can realize machine testing on whole blood；
6. the analyzer of the present invention is small, can be portable, particularly suitable private doctor makes house calls.
Description of the drawings
With reference to attached drawing, the disclosure will be easier to understand.Skilled addressee readily understands that be：This
A little attached drawings are used only for the technical solution illustrated the present invention, and are not intended to and are construed as limiting to protection scope of the present invention.
In figure：
Fig. 1 is the structural schematic diagram of the biochemical immunity analyzer of the embodiment of the present invention；
Fig. 2 is the structural schematic diagram of the integrated apparatus based on multiple check bits of the embodiment of the present invention；
Fig. 3 be the rotation axis of the embodiment of the present invention, pallet structural schematic diagram；
Fig. 4 is the structural schematic diagram of the rotation axis of the embodiment of the present invention；
Fig. 5 is the structural schematic diagram of the pallet of the embodiment of the present invention.
Specific implementation mode
Fig. 1-5 and following description describe the present invention optional embodiment to instruct how those skilled in the art implement
It is of the invention with reproducing.In order to instruct technical solution of the present invention, some conventional aspects are simplified or have been omitted.Those skilled in the art
It should be appreciated that modification or replacement from these embodiments will within the scope of the invention.Those skilled in the art should understand that
Following characteristics can be combined in various ways to form multiple modifications of the present invention.As a result, the invention is not limited in it is following can
Embodiment is selected, and is only limited by the claims and their equivalents.
Embodiment 1：
Fig. 1 schematically illustrates a kind of structure diagram of biochemical immunity analyzer of the embodiment of the present invention, such as Fig. 1 institutes
Show, the biochemical immunity analyzer includes：
Integrated biochemical device, Fig. 2 schematically illustrate the integration based on multiple check bits of the embodiment of the present invention
The structure diagram of biochemical device, as shown in Fig. 2, the integration biochemical device includes：
Ontology 1, the ontology have the fixed part 2 for being suitable for connecting with the shaft of motor etc., for extraneous dynamic in motor etc.
It is rotated under power drive；It is formed on the ontology：
Sap cavity 3 is diluted, the dilution sap cavity 3 is arranged around the outer rim of the fixed part 2；The dilution sap cavity 3 is pre-installed
Dilution, and with outlet 7；The leakage when being not used, the outlet can also install sealer additional in order to prevent, use dilution
It is torn in liquid；
First quantitative chamber 6, the described first quantitative chamber 6 are arranged in the periphery of the dilution sap cavity 3, and with the dilution sap cavity
3 connections；One end for facing body central of described first quantitative chamber 6 has the first overflow port 15；Described first quantitative chamber 6 passes through
First passage 12 is connected to mixing chamber 8；
Sample cavity 10, the sample cavity 10 are arranged in the periphery of the dilution sap cavity 3；
Second quantitative chamber 4, the described second quantitative chamber 4 are arranged in the periphery of the sample cavity 10, and with the sample cavity 10
Connection；There is the second overflow port 14, the described second quantitative chamber 4 to pass through for one end for facing body central of described second quantitative chamber 4
Second channel 11 is connected to mixing chamber 8；
First storage chamber 5, first storage chamber 5 are arranged in the periphery of the described second quantitative chamber 4, and determine with described second
Chamber 4 is measured to be connected to；
Second storage chamber, second storage chamber are connected to second overflow port；
Third storage chamber, the third storage chamber are connected to first overflow port；
First passage 12, the first passage 12 have reducing-flow structure, microfluidic valve are such as arranged on a passage, or use tool
There is the capillary of bending structure；
Second channel 11, the second channel 11 have reducing-flow structure, microfluidic valve are such as arranged on a passage, or use tool
There is the capillary of bending structure；
Mixing chamber 8, the mixing chamber 8 is arranged in the periphery of the described first quantitative chamber 6, and passes through the connection point of third channel 13
With chamber；
Third channel 13, the third channel 13 have reducing-flow structure, microfluidic valve are such as arranged on a passage, or use tool
There is the capillary of bending structure；
Distribution cavity, the distribution cavity are arranged in the periphery of the mixing chamber 8, and are connected to multiple test chambers 9 by channel；
Multiple test chambers 9, the test chamber are arranged in the periphery of the distribution cavity；
There is opening, inside cavity guide rail is arranged for cavity 81, the cavity, the left and right sides setting in cavity as described below
Linear guide；
There is rotation axis, pallet, holder and block piece, the holder to be arranged on the guide rail for bracket 82, the bracket,
On the bracket, the pallet is fixed in the rotation axis, and the rotation axis passes through the one for the rotation axis setting
Change the through-hole of biochemical device, the pallet carrying integrated biochemical device, the block piece setting on the bracket, is used for
Bracket blocks the opening when entering in the cavity；
First driving unit, first driving unit is for driving the holder to be moved on the guide rail；
Second driving unit, second driving unit is for driving the rotation axis to rotate；
First light source 83, the first measurement light that the first light source is sent out are adapted to pass through on the integrated biochemical device
Test chamber；Based on this, the first light source and the first detector are arranged on the cavity, and are respectively at one in the cavity
The upper side and lower side of body biochemical device；
Second light source 85, the second measurement light that the second light source is sent out are suitable for irradiating on the integrated biochemical device
Test chamber；Based on this, the second light source and the second detector are arranged on the side wall of the cavity；
First detector 84, first detector are used to receive the first measurement light after the test chamber；
Second detector 86, second detector receive second and measure scattering of the light on the detection intracavity liquid
Light.
In order to enhance integrated biochemical device and the rotation fixed reliability of between centers, prevent integrated biochemical device from rotating
It is shaken on axis, further, the rotation axis is flower pattern axis, and the inner wall of the through-hole is matched with the rotation axis outer rim.
In order to enhance pallet and the rotation fixed reliability of between centers, prevent pallet from shaking on the rotating shaft, further, such as
Shown in Fig. 3-5, the pallet 23 is sleeved in the rotation axis 22, and pallet 23 has the matched through-hole of outer rim with rotation axis 22；
The pallet is integrated with rotation axis or is separately provided.
Integrated biochemical device is thrown out of in rotary course in order to prevent, further, as in Figure 3-5, the biochemistry
Immunity analysis instrument further comprises：
Elastic component 25, the elastic component are arranged in the inside of the rotation axis；
Movable part 26 connects being partially in outside the rotation axis outer rim for the movable part of the elastic component, exposed parts tool
There are curved surfaces.
In order to improve the manufacture efficiency of integrated biochemical device, the ontology includes：
First part, the first part and second part are setting up and down；There is through-hole, the position of the through-hole in first part
Set it is corresponding with the sample cavity position of second part, to by sample be added sample cavity in；
Second part, the dilution sap cavity, sample cavity, the first quantitative chamber, the second quantitative chamber, mixing chamber, the first storage chamber,
Second storage chamber, third storage chamber, first passage, second channel, third channel, distribution cavity and test chamber are formed in described second
On part, then such as cutting on the second portion is tightly connected with first part, to form each chamber, channel.
The analysis method of the above-mentioned biochemical immunity analyzer of the embodiment of the present invention, the analysis method include the following steps：
(C1) it places on the rotating shaft added with the integrated biochemical device of sample, and is carried by pallet；The one metaplasia
Disguising the modes of emplacement set is：
(B1) integrated biochemical device is placed, the through-hole of the integration biochemical device is against the rotation axis；
(B2) the integrated biochemical device is pressed down on, the protrusion rotation axis outer rim of the movable part in the rotation axis
Part is squeezed and retreated towards the inside of rotation axis by the inner wall of the through-hole of the integrated biochemical device；
When the integration biochemical device is moved down under the movable part, the movable part is under elastic component effect
Rebound, the part of movable part protrudes from rotation axis outer rim；
(B3) the integrated biochemical device is moved down along the rotation axis, the through-hole of the integration biochemical device
Inner wall matched with the outer wall of the rotation axis；
(C2) under the effect of the first driving unit, the integration biochemical device moves simultaneously with the holder on guide rail
Into in the cavity, the block piece blocks the outlet of the cavity；
(C3) under the effect of the second driving unit, the integration biochemical device is rotated with different rotating speeds so that described one
The dilution mixing pre-installed in sample in body biochemical device and integrated biochemical device, later in test chamber and prepackage
Reagent reacts；Concrete mode is：
(A1) integrated biochemical device is rotated with the first rotating speed, and the sample in feeding chamber enters the second quantitative chamber and first and deposits
In storage chamber, the larger substance of density enters in the first storage chamber in sample, and the smaller liquid of density enters the second quantitative chamber；It is extra
Sample flowed out from the second overflow port, and enter the second storage chamber in；It is larger that the volume of first storage chamber is more than density in sample
The volume of substance；
Meanwhile the dilution of dilution intracavitary prepackage is thrown away from the outlet, into the first quantitative intracavitary, extra is dilute
It releases liquid to flow out by the first overflow port, and enters in third storage chamber；If exit has sealer, then need to tear sealer；
(A2) integrated biochemical device stops rotating, hydraulically full in the first passage and second channel；
(A3) integrated biochemical device is rotated with the second rotating speed, and the first quantitative intracavitary dilution is entered mixed by first passage
Chamber is closed, the liquid that the density of the described second quantitative intracavitary is smaller enters the mixing chamber；
(A4) integrated biochemical device enters the cycle stage of acceleration and deceleration, is converted by multiple high speed low speed so that sample
Density smaller liquid and dilution mix in mixing chamber in this；
(A5) integrated biochemical device stops rotating, hydraulically full in the third channel；
(A6) integrated biochemical device is rotated with third rotating speed, and the liquid in mixing chamber enters distribution cavity by third channel
It is interior, enter in each test chamber later, liquid dissolves the reagent pre-installed in test chamber, such as the reagent after being freeze-dried；
(A7) integrated biochemical device is rotated with the 4th rotating speed, and the 4th rotating speed is less than the first rotating speed, the second rotating speed and third
Rotating speed, the liquid in multiple test chambers are reacted in isoperibol；
(C4) the first measurement light and the second measurement illumination are mapped to the liquid in test chamber, and are received；Pass through analysis first
The output signal of detector and the second detector is such as detected using colorimetric method, turbidimetry and scattered light urbidmetry, to know
The parameter of sample.
Embodiment 2：
Application examples of the according to embodiments of the present invention 1 biochemical immunity analyzer and analysis method in biochemical project.
In the application examples, as shown in Figs. 1-5, ontology uses resin and plastic, by first part setting up and down and second
Part forms, and quantitative chamber, mixing chamber, test chamber etc. are formed on the second portion by cutting；Dilution and reagent are pre-installed
In integrated biochemical device；First passage uses the capillary with bending structure, the rotation center of ontology to described first
The minimum range in channel is less than the rotation center of the ontology to the minimum range of the described first quantitative chamber；Second channel is using tool
There are the capillary of bending structure, the rotation center of the ontology to be less than the rotation of the ontology to the minimum range of the second channel
Turn the minimum range that center quantifies chamber to described second；Third channel uses the capillary with bending structure, the ontology
The minimum range of rotation center to the third channel is less than the rotation center of the ontology to the most narrow spacing of the mixing chamber
From；There is the outer rim of the rotation axis 22 direction along the axis for being parallel to the rotation axis being evenly spaced on to extend
Arc convex 27, such as 5 protrusions, raised top and bottom have chamfering 28, are smoothly caught in convenient for integrated biochemical device；With
Accordingly, the inner wall of the through-hole of the ontology has and protrusion matched equally distributed groove protrusion 27；Movable part 26 is 2
It is a, it is spherical shape, there is rotation axis 22 through-hole 29, the movable part to resist the through-hole of rotation axis；The diameter of the through-hole is less than
The diameter of the elastic component；Elastic component uses spring；Throwing of the movable part in the plane perpendicular to rotation axis central axis
Shadow is between the projection of adjacent protrusion on the plane；Matchingly with the protrusion 27 of 22 outer rim of rotation axis, pallet 23
The inner wall of through-hole has groove 31 so that pallet 23 is stuck in the rotation axis 22；Screw 24 sequentially passes through logical on pallet 23
Through-hole 20 on hole 32 and rotation axis, and the shaft of motor 21 is headed on, to which rotation axis 22 and pallet 23 are firmly secured to
On machine shaft.
According to the analysis method of above-mentioned biochemical immunity analyzer, (C3) is specially the step of the analysis method：
(A1) fixed part connect and fixes with machine shaft, micro sample to be tested is added in the feeding chamber, such as
0.1ml whole bloods；Tear sealer；
Integrated biochemical device is rotated with the first rotating speed, and the sample in feeding chamber enters the second quantitative chamber and the first storage chamber
Interior, the larger substance of density enters in the first storage chamber in sample, and the smaller liquid of density enters the second quantitative chamber；Extra sample
This is flowed out from the second overflow port, and is entered in the second storage chamber；The volume of first storage chamber is more than the larger substance of density in sample
Volume；
Meanwhile the dilution of dilution intracavitary prepackage is thrown away from the outlet, into the first quantitative intracavitary, extra is dilute
It releases liquid to flow out by the first overflow port, and enters in third storage chamber；
(A2) integrated biochemical device stops rotating, and is full of due to capillarity in the first passage and second channel
Liquid；
(A3) integrated biochemical device is rotated with the second rotating speed, and the first quantitative intracavitary dilution is entered mixed by first passage
Chamber is closed, the liquid that the density of the described second quantitative intracavitary is smaller enters the mixing chamber；
(A4) integrated biochemical device enters the cycle stage of acceleration and deceleration, is converted by multiple high speed low speed so that sample
Density smaller liquid and dilution mix in mixing chamber in this；
(A5) integrated biochemical device stops rotating, hydraulically full due to capillarity in the third channel；
(A6) integrated biochemical device is rotated with third rotating speed, and the liquid in mixing chamber is logical by third due to siphonage
Road enters in distribution cavity, enters later in each test chamber of a circle, and liquid dissolves the reagent pre-installed in test chamber, such as freezes dry
Reagent after dry；
(A7) integrated biochemical device is rotated with the 4th rotating speed, and the 4th rotating speed is less than the first rotating speed, the second rotating speed and third
Rotating speed, the liquid in multiple test chambers are reacted in isoperibol.
Embodiment 3：
Application examples of the according to embodiments of the present invention 1 biochemical immunity analyzer and analysis method in immunization program.
As different from Example 2：Reducing-flow structure uses microfluidic valve, and after the rotating speed of rotation reaches certain value, liquid is
Microfluidic valve is broken through to enter in next cavity.
Claims (10)
1. a kind of integrated apparatus based on multiple check bits, the integrated apparatus include ontology, the ontology, which has, to be suitable for
It is connected with the rotating shaft fixed part；It is characterized in that：The integrated apparatus further comprises being formed on the body：
Sap cavity is diluted, the dilution sap cavity is arranged around the outer rim of the fixed part；The dilution sap cavity has outlet；
First quantitative chamber, the described first quantitative chamber is arranged in the periphery of the dilution sap cavity, and is connected to the dilution sap cavity；Institute
The one end for facing body central for stating the first quantitative chamber has the first overflow port；Described first quantitative chamber is by first passage and mixes
Close chamber connection；
Sample cavity, the sample cavity are arranged in the periphery of the dilution sap cavity；
Second quantitative chamber, the described second quantitative chamber is arranged in the periphery of the sample cavity, and is connected to the sample cavity；Described
There is the second overflow port, the described second quantitative chamber to pass through second channel and mixing chamber for one end for facing body central of two quantitative chambers
Connection；
First storage chamber, first storage chamber are arranged in the periphery of the described second quantitative chamber, and connect with the described second quantitative chamber
It is logical；
Second storage chamber, second storage chamber are connected to second overflow port；
Third storage chamber, the third storage chamber are connected to first overflow port；
First passage, the first passage have reducing-flow structure；
Second channel, the second channel have reducing-flow structure；
Mixing chamber, the mixing chamber is arranged in the periphery of the described first quantitative chamber, and is connected to distribution cavity by third channel；
Third channel, the third channel have reducing-flow structure；
Distribution cavity, the distribution cavity are arranged in the periphery of the mixing chamber, and are connected to multiple test chambers by channel；
Multiple test chambers, the test chamber are arranged in the periphery of the distribution cavity.
2. integrated apparatus according to claim 1, it is characterised in that：The ontology includes：
First part, the first part and second part are setting up and down；
Second part, the dilution sap cavity, sample cavity, the first quantitative chamber, the second quantitative chamber, mixing chamber, the first storage chamber, second
Storage chamber, third storage chamber, first passage, second channel, third channel, distribution cavity and test chamber are formed in the second part
On.
3. integrated apparatus according to claim 1, it is characterised in that：The outlet has sealer.
4. integrated apparatus according to claim 1, it is characterised in that：The dilution intracavitary envelope has dilution.
5. integrated apparatus according to claim 1, it is characterised in that：
The first passage uses the capillary with bending structure, the rotation center of the ontology to the first passage most
Small distance is less than the rotation center of the ontology to the minimum range of the described first quantitative chamber；Alternatively,
The second channel uses the capillary with bending structure, the rotation center of the ontology to the second channel most
Small distance is less than the rotation center of the ontology to the minimum range of the described second quantitative chamber；Alternatively,
The third channel uses the capillary with bending structure, the rotation center of the ontology to the third channel most
Small distance is less than the rotation center of the ontology to the minimum range of the mixing chamber.
6. a kind of biochemical immunity analyzer, the biochemical immunity analyzer includes light source, detector；It is characterized in that：The life
Change immunity analysis instrument to further comprise：
Integrated apparatus, the integrated apparatus is using any integrated apparatus of claim 1-5.
7. the analysis method of biochemical immunity analyzer according to claim 6, the analysis method include the following steps：
(A1) integrated apparatus in shaft is fixed on to rotate with the first rotating speed, the sample in feeding chamber enter the second quantitative chamber and
In first storage chamber, the larger substance of density enters in the first storage chamber in sample, and it is quantitative that the smaller liquid of density enters second
Chamber；Extra sample is flowed out from the second overflow port, and is entered in the second storage chamber；
Meanwhile the dilution of dilution intracavitary prepackage is thrown away from the outlet, into the first quantitative intracavitary, extra dilution
It is flowed out, and entered in third storage chamber by the first overflow port；
(A2) integrated apparatus stops rotating, hydraulically full in the first passage and second channel；
(A3) integrated apparatus is rotated with the second rotating speed, and the first quantitative intracavitary dilution enters mixing chamber by first passage, institute
The smaller liquid of density for stating the second quantitative intracavitary enters the mixing chamber；
(A4) integrated apparatus enters the cycle stage of acceleration and deceleration, is converted by multiple high speed low speed so that density in sample
Smaller liquid and dilution mixes in mixing chamber；
(A5) integrated apparatus stops rotating, hydraulically full in the third channel；
(A6) integrated apparatus is rotated with third rotating speed, and the liquid in mixing chamber enters by third channel in distribution cavity, later
Into in each test chamber, liquid dissolves the reagent pre-installed in test chamber；
(A7) integrated apparatus is rotated with the 4th rotating speed, and the 4th rotating speed is less than the first rotating speed, the second rotating speed and third rotating speed, detection
The liquid of intracavitary is reacted in isoperibol, and is detected by optical signalling.
8. analysis method according to claim 7, it is characterised in that：The volume of first storage chamber be more than sample in density compared with
The volume of big substance.
9. analysis method according to claim 7, it is characterised in that：In step (A2), the envelope in the exit is torn
Film.
10. analysis method according to claim 7, it is characterised in that：In step (A7), colorimetric method, transmittance are utilized
Turbid method and scattered light urbidmetry detection.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810423060 | 2018-05-05 | ||
| CN2018104230602 | 2018-05-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810782047.6A Pending CN108646015A (en) | 2018-05-05 | 2018-07-17 | Integrated apparatus based on multiple check bits and analysis method |
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Application publication date: 20181012 |