CN109613287A - A kind of quantitative biochip reaction apparatus - Google Patents
A kind of quantitative biochip reaction apparatus Download PDFInfo
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- CN109613287A CN109613287A CN201811607087.3A CN201811607087A CN109613287A CN 109613287 A CN109613287 A CN 109613287A CN 201811607087 A CN201811607087 A CN 201811607087A CN 109613287 A CN109613287 A CN 109613287A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00158—Elements containing microarrays, i.e. "biochip"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00277—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)
- G01N2035/00287—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material) movable lid/cover for sample or reaction tubes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
- G01N2035/00376—Conductive heating, e.g. heated plates
Abstract
The invention discloses a kind of quantitative biochip reaction apparatus, feature be include reaction instrument ontology and biochip, reacting instrument ontology includes reaction cabinet, a reaction box is provided in reaction chamber body, reaction chamber is provided in reaction box, it is provided with the lid for covering reaction chamber on reaction box turnablely, the useful heating mechanism heated to reaction chamber of setting in reaction box is provided in reaction box and shelves mechanism for shelve biochip.Advantage is by shelving mechanism for shelving biochip, biochip is stably placed in reaction box, is heated by heating mechanism to reaction chamber, so that the reaction to biology to be measured on biochip provides a suitable reaction temperature, structure is simple, good portability.
Description
Technical field
The present invention relates to biochip test fields, more particularly, to a kind of quantitative biochip reaction apparatus.
Background technique
Biochip (biochip or bioarray) be according to the principle specifically to interact between biomolecule, will be biochemical
Analytic process is integrated in chip surface, to realize the high throughput to DNA, RNA, polypeptide, protein and other biological ingredient
Quickly detection.The biochip concept of narrow sense refers to biomolecule (oligonucleotides, cDNA, genomic through distinct methods
DNA, polypeptide, antibody, antigen etc.) it is bonded in the solid phase mediators such as silicon wafer, sheet glass (pearl), plastic sheet (pearl), gel, nylon membrane
The biomolecule dot matrix of formation.The concept of chip takes from integrated concept, and biochip is the integrated of biomaterial.As experiment
Room detection is the same, and blood glucose, albumen, enzymatic activity etc. are checked on biochip, is based on same biological respinse principle.So raw
Object chip is exactly a carrier platform, is a carrier, is that analytic process discontinuous in life science is integrated in base
The miniature biochemical analysis system on piece surface, to realize to the accurate, fast of cell, protein, gene and other biological components
The detection of speed, large information capacity.The technical advantage that biochip has keeps detection quicker, sensitive and accurate.
At present when reacting biological sample, it usually needs use nucleic acid augmentative instrument, chip hybridization instrument, chip clear
The equipment such as washing machine, and above-mentioned a whole set of related equipment currently on the market is not only expensive, and volume is big and heavy, it is unsuitable
It is often mobile and mobile difficult larger.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of structures simply, the quantitative biochip reaction of good portability
Instrument.
The technical scheme of the invention to solve the technical problem is:
A kind of quantitative biochip reaction apparatus, including reaction instrument ontology and biochip, the reaction instrument ontology include reaction
Cabinet is provided at least one reaction box in the reaction chamber body, is provided with reaction chamber in each reaction box, described
Reaction box on be provided with lid for covering the reaction chamber turnablely, setting is useful in the reaction box
It is provided in the heating mechanism to the reaction chamber heating, the reaction box for shelving putting for the biochip
Set mechanism.
The biochip includes a transparent reaction disk, is circumferentially radially arranged in the reaction disk
There are multiple conversion zones, each conversion zone includes the liquid cell and reaction tank set gradually from inside to outside, described
It is connected between liquid cell and reaction tank by a conduit, in the center and each conversion zone of the reaction disk
The center of the liquid cell, the line between the center of the reaction tank it is mutually be overlapped with the central axis of the conduit
Close, be provided with multiple wells on the reaction disk, be provided with and communicated with one on each liquid cell described in plus
Sample hole is provided with multiple gas vents on the reaction disk, and the side of each reaction tank is provided with and communicated with an institute
The gas vent stated.By the multiple conversion zones being arranged on reaction disk, so that the biochip can be carried out in the same time
Multiple groups reaction, improves operating efficiency, and each conversion zone includes the liquid cell and reaction tank set gradually from inside to outside, liquid
It is connected between body pond and reaction tank by a conduit, well is provided on liquid cell, biological sample and reaction reagent passes through
Well is added in liquid cell, then applies a rotatory force to reaction disk, biological sample and reaction examination in liquid cell
Agent can enter reaction tank by conduit under the action of the rotatory force and be reacted, and add other reagents in the process,
The reagent is equally then added to liquid cell by well, then by applying rotatory force to reaction disk, so that the reagent
It being entered in reaction tank by conduit, structure is simple, and it is easy to operate, effectively prevent biological sample quilt during reagent dropwise
Pollution, to obtain more accurately experimental data, the setting of gas vent enable biological sample and reaction reagent smoothly into
Enter to reaction tank.
It is provided between the liquid cell and the reaction tank for preventing liquid from flowing back to stopping for the liquid cell
Hui Chi, the conduit are divided into the first catheter segment and the second catheter segment, pass through between the liquid cell and the non-return pond
First catheter segment is connected, and is connected between the non-return pond and the reaction tank by second catheter segment
It is logical, the center of the reaction disk with each described in conversion zone in the liquid cell center, described react
The central axis of line and the conduit between the center in pond and the center in the non-return pond coincides, the non-return
The width in pond is greater than the width of the conduit.The liquid that the setting in the non-return pond of above structure can be effectively prevent in reaction tank returns
It flows in liquid cell, so that it is guaranteed that reaction is normally carried out, structure is simple, uses stabilization.
It is described shelve mechanism include one it is vertically arranged wear spindle, a motor, institute are provided in the reaction chamber body
The lower section of the reaction box is arranged in the motor stated, and the output shaft of the motor is vertically arranged, and described wears under spindle
The output shaft that the reaction box and the motor are stretched out in end is fixedly and coaxially connected, and the spindle of wearing connects column certainly by multiple wear
It is connected in sequence under above, the side outwardly convex that described wearing connects column is provided with anti-rotation convex block, the reaction disk
Central coaxial, which is provided with, connects the mounting hole that column is coupled with described wearing, and is provided in the mounting hole and the anti-rotation
The anti-rotation groove that convex block matches, the top and bottom that described wearing connects column are respectively arranged with coaxial threaded connection section, up and down
It wears to connect described in adjacent two and be connected between column by a limited post, is provided on the limited post and the screw thread
The internal thread hole that linkage section matches, the outer diameter of the limited post are greater than the outer diameter that described wearing connects column.Above-mentioned design so that
Reaction box disposably can shelve multiple reaction disks simultaneously, so that reaction efficiency is greatly improved；Pass through motor
Rotation provide a centrifugal force to reaction disk, by the effect of centrifugal force so that biological sample and reaction in liquid cell
Reagent quickly and steadily can be entered in reaction tank and be reacted, easy to operate and quick.
The heating mechanism includes heating plate, and the heating plate is circumferentially positioned on the inner wall of the reaction box.
Heating plate is used to provide environment temperature to the reaction of biological sample, to promote to react relatively stable and carry out well.
Be provided with control circuit in the reaction chamber body, the motor and the heating plate with the control
Circuit is electrically connected, and is additionally provided with temperature sensor in the reaction chamber, the temperature probe of the temperature sensor, which protrudes into, to be set
It sets in the reaction box, the temperature sensor is electrically connected with the control circuit.Control circuit is for controlling electricity
Rotation, heating of heating plate of machine etc.；Temperature sensor is for detecting the intracorporal temperature of reaction chamber, to drive control circuit control
Heating sheet processed provides more suitable reaction temperature.
It is provided with multiple heat release holes on the reaction cabinet, is provided with radiator fan in the reaction chamber body, it is described
Radiator fan be electrically connected with the control circuit.It being used to give reaction cabinet cooling by above-mentioned radiator structure, structure is simple,
Good heat dissipation effect.
Operating display is provided on the reaction cabinet, the operating display is electrically connected with the control circuit
It connects.Operating display is convenient for operation of the user to entire reaction controlling, such as the rotation of control motor, heating plate to begin to warm up
Operation.
The lower end for wearing spindle is coaxially connected with a connecting shaft, and the connecting shaft stretches out the reaction box and institute
The output shaft for the motor stated is fixedly connected, and the top of the connecting shaft is provided with the outer spiral shell matched with the internal thread hole
Line.Being fixedly connected between spindle and motor output shaft is worn by connecting shaft realization, structure is simple, easy for installation.
Compared with the prior art, the advantages of the present invention are as follows: by shelving mechanism for shelving biochip, so that biological
Chip can be stably placed in reaction box, be heated by heating mechanism to reaction chamber, thus to biology to be measured on biochip
Reaction provides a suitable reaction temperature, and structure is simple, good portability.
Detailed description of the invention
Fig. 1 is the overall structure diagram of the embodiment of the present invention one；
Fig. 2 is the first schematic cross-sectional view of the embodiment of the present invention one；
Fig. 3 is the second schematic cross-sectional view of the embodiment of the present invention one；
Fig. 4 is to wear the schematic perspective view for connecing column in the embodiment of the present invention one；
Fig. 5 is the structural schematic diagram of biochip in the embodiment of the present invention one；
Fig. 6 is the overall structure diagram of the embodiment of the present invention two.
Specific embodiment
The present invention will be described in further detail below with reference to the embodiments of the drawings.
Embodiment one: as shown in Figures 1 to 5, a kind of quantitative biochip reaction apparatus, including reaction instrument ontology B and biology
Chip A, reaction instrument ontology B include reaction cabinet B1, react in cabinet B1 and are provided with a reaction box B5, are provided in reaction box B5
The lid B52 for covering reaction chamber B51 is provided on reaction chamber B51, reaction box B5 turnablely, is set in reaction box B5
It is equipped with the heating mechanism for heating to reaction chamber B51, is provided in reaction box B5 and shelves machine for shelve biochip A
Structure.
In this particular embodiment, biochip A includes a transparent reaction disk A1, is circumferentially in reaction disk A1
Radial to be provided with multiple conversion zone A2, each conversion zone A2 includes the liquid cell A21 set gradually from inside to outside and anti-
Pond A22 is answered, is connected between liquid cell A21 and reaction tank A22 by a conduit, the center of reaction disk A1 and each reaction zone
The central axis of line and conduit between the center of liquid cell A21 in the A2 of domain, the center of reaction tank A22 coincides, reaction
It is provided with multiple well A11 on disk A1, a well A11 is provided with and communicated on each liquid cell A21, reacts disk A1
On be provided with multiple gas vent A12, the side of each reaction tank A22 is provided with and communicated with a gas vent A12.By justifying in reaction
The multiple conversion zone A2 being arranged on disk A1 improve operation so that biochip A can carry out multiple groups reaction in the same time
Efficiency, and each conversion zone A2 includes the liquid cell A21 and reaction tank A22 set gradually from inside to outside, liquid cell A21 and anti-
It answers and is connected between the A22 of pond by a conduit, well A11 is provided on liquid cell A21, biological sample and reaction reagent pass through
Well A11 is added in liquid cell A21, then applies a rotatory force to reaction disk A1, the biological sample in liquid cell A21
This and reaction reagent can enter reaction tank A22 by conduit under the action of the rotatory force and be reacted, and in the process
Other reagents are added, then the reagent are equally added to liquid cell A21 by well A11, then by giving reaction disk A1
Apply rotatory force, so that the reagent is entered in reaction tank A22 by conduit, structure is simple, it is easy to operate, it effectively prevents giving birth to
Object sample is contaminated during reagent dropwise, to obtain more accurately experimental data, the setting of gas vent A12 is so that raw
Object sample and reaction reagent can smoothly enter into reaction tank.
In this particular embodiment, it is provided between liquid cell A21 and reaction tank A22 for preventing liquid from flowing back to liquid
The non-return pond A23 of pond A21, conduit are divided into the first catheter segment A24 and the second catheter segment A25, liquid cell A21 and non-return pond A23 it
Between be connected by the first catheter segment A24, be connected between non-return pond A23 and reaction tank A22 by the second catheter segment A25, instead
The center for answering disk A1 and the center of liquid cell A21, the center of reaction tank A22 and non-return pond A23 in each conversion zone A2
Center between line and the central axis of conduit coincide, the width of non-return pond A23 is greater than the width of conduit.Above structure
The setting of non-return pond A23 the liquid reflux in reaction tank A22 can be effectively prevent into liquid cell A21, so that it is guaranteed that reaction
It is normally carried out, structure is simple, uses stabilization.
In this particular embodiment, shelve mechanism include one it is vertically arranged wear spindle B2, react and be provided in cabinet B1
The lower section of reaction box B5 is arranged in one motor B3, motor B3, and the output shaft B31 of motor B3 is vertically arranged, wears the lower end of spindle B2
The output shaft B31 for stretching out reaction box B5 and motor B3 is fixedly and coaxially connected, and is worn spindle B2 by identical wear of multiple structures and is met column B21
It is connected in sequence from top to bottom, wears and connect the side outwardly convex of column B21 and be provided with anti-rotation convex block B211, react in disk A1
The heart, which is coaxially arranged with and wears, meets the mounting hole A13 that column B21 is coupled, and is provided in mounting hole A13 and anti-rotation convex block B211 phase
The anti-rotation groove A131 of cooperation wears and connects the upper end of column B21 and be coaxially arranged with externally threaded connecting column B212, wears and meet column B21
Upper end be coaxially arranged with limited post B213, the internal thread hole matched with connecting column B212 is provided on limited post B213, limit
The outer diameter of position column B213, which is greater than, wears the outer diameter for meeting column B21.
In this particular embodiment, heating mechanism includes heating plate (not shown), and heating plate is circumferentially positioned at reaction
On the inner wall of box B5.
In this particular embodiment, it reacts and is provided with control circuit (not shown) in cabinet B1, motor B3 and heating
Plate is electrically connected with control circuit, and temperature sensor B4 is additionally provided in reaction chamber B51, and the temperature probe of temperature sensor B4 is stretched
Enter to be arranged in reaction box B5, temperature sensor B4 is electrically connected with control circuit.
In this particular embodiment, it reacts and is provided with multiple heat release hole B11 on cabinet B1, be provided in reaction cabinet B1 scattered
Hot-air fan B12, radiator fan B12 are electrically connected with control circuit.
In this particular embodiment, it reacts and is provided with operating display B13 on cabinet B1, operating display B13 and control
Circuit electrical connection.
In this particular embodiment, the lower end for wearing spindle B2 is coaxially connected with a connecting shaft B6, and connecting shaft B6 stretches out reaction
Box B5 is fixedly connected with the output shaft B31 of motor B3, and the top of connecting shaft B6 is provided with the external screw thread matched with internal thread hole.
Embodiment two: as shown in fig. 6, other parts are the same as example 1, the difference is that in reaction cabinet B1
Reaction box B5 there are three being set side by side.Multiple reaction box B5 are provided in reaction cabinet B1, reaction efficiency can be effectively improved.
In this particular embodiment, lid B52 is transparent cover body, convenient for the reaction of biochip A in observing response box B5
Situation.
Claims (9)
1. a kind of quantitative biochip reaction apparatus, it is characterised in that including reaction instrument ontology and biochip, the reaction instrument
Ontology includes reaction cabinet, is provided at least one reaction box in the reaction chamber body, setting in each reaction box
There is reaction chamber, is provided with the lid for covering the reaction chamber on each reaction box turnablely, it is described
Reaction box in be provided with heating mechanism for heating to the reaction chamber, be provided in the reaction box for shelving
The biochip shelves mechanism.
2. a kind of quantitative biochip reaction apparatus as described in claim 1, it is characterised in that the biochip includes one
Transparent reaction disk is circumferentially radially provided with multiple conversion zones in the reaction disk, each described anti-
Answering region includes the liquid cell and reaction tank set gradually from inside to outside, passes through a conduit between the liquid cell and reaction tank
It is connected, it is the center of the center of the described reaction disk and the liquid cell in each conversion zone, described
The central axis of line and the conduit between the center of reaction tank coincides, and is provided on the reaction disk multiple
Well, be provided with and communicated with one on the liquid cell described in well, be provided on the reaction disk more
A gas vent, the side of the reaction tank be provided with and communicated with one described in gas vent.
3. a kind of quantitative biochip reaction apparatus as claimed in claim 2, it is characterised in that the liquid cell and described
The non-return pond for preventing liquid from flowing back to the liquid cell is provided between reaction tank, the conduit is divided into the first catheter segment
With the second catheter segment, it is connected between the liquid cell and the non-return pond by first catheter segment, it is described
It is connected between non-return pond and the reaction tank by second catheter segment, the center of the described reaction disk and each
The center at the center of the liquid cell in the conversion zone, the center of the reaction tank and the non-return pond it
Between the central axis of line and the conduit coincide, the width in the non-return pond is greater than the width of the conduit.
4. a kind of quantitative biochip reaction apparatus as claimed in claim 2, it is characterised in that the mechanism of shelving includes one
Vertically arranged wears spindle, a motor is provided in the reaction chamber body, the reaction box is arranged in the motor
The output shaft of lower section, the motor is vertically arranged, and the reaction box and the electricity are stretched out in the lower end for wearing spindle
The output shaft of machine is fixedly and coaxially connected, it is described wear spindle by multiple structures it is identical wear connect column be sequentially connected from top to bottom and
, the side outwardly convex that described wearing connects column is provided with anti-rotation convex block, the central coaxial of the reaction disk be provided with
Described wearing connects the mounting hole that column is coupled, and the anti-rotation matched with the anti-rotation convex block is provided in the mounting hole
Groove, the upper end that described wearing connects column are coaxially arranged with externally threaded connecting column, and the upper end that described wearing connects column is coaxially set
It is equipped with limited post, the internal thread hole matched with the connecting column is provided on the limited post, the limited post
Outer diameter is greater than the outer diameter that described wearing connects column.
5. a kind of quantitative biochip reaction apparatus as described in claim 1, it is characterised in that the heating mechanism includes adding
Hot plate, the heating plate are circumferentially positioned on the inner wall of the reaction box.
6. a kind of quantitative biochip reaction apparatus as claimed in claim 5, it is characterised in that setting in the reaction chamber body
There is control circuit, the motor is electrically connected with the control circuit with the heating plate, in the reaction chamber also
It is provided with temperature sensor, the temperature probe of the temperature sensor, which protrudes into, to be arranged in the reaction box, the temperature
Degree sensor is electrically connected with the control circuit.
7. a kind of quantitative biochip reaction apparatus as claimed in claim 6, it is characterised in that be arranged on the reaction cabinet
There are multiple heat release holes, radiator fan, the radiator fan and control circuit electricity are provided in the reaction chamber body
Connection.
8. a kind of quantitative biochip reaction apparatus as claimed in claim 6, it is characterised in that be arranged on the reaction cabinet
There is operating display, the operating display is electrically connected with the control circuit.
9. a kind of quantitative biochip reaction apparatus as claimed in claim 4, it is characterised in that the lower end for wearing spindle is same
Axis connection has a connecting shaft, and the connecting shaft is stretched out the reaction box and is fixedly connected with the output shaft of the motor, institute
The top for the connecting shaft stated is provided with the external screw thread matched with the internal thread hole.
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Effective date of registration: 20210125 Address after: 315040 Ningbo New Materials Innovation Center East District, Ningbo High-tech Zone, Zhejiang Province, No. 1 11-2-1 Applicant after: Zhejiang Zhenghegu Biotechnology Co.,Ltd. Address before: 315211, Fenghua Road, Jiangbei District, Zhejiang, Ningbo 818 Applicant before: Ningbo University |