CN209745988U

CN209745988U - Qualitative/quantitative biochip detection device

Info

Publication number: CN209745988U
Application number: CN201822213631.8U
Authority: CN
Inventors: 周君; 苏秀榕; 芦晨阳; 李妍妍
Original assignee: Ningbo University
Current assignee: Zhejiang Zhenghegu Biotechnology Co.,Ltd.
Priority date: 2018-12-27
Filing date: 2018-12-27
Publication date: 2019-12-06
Anticipated expiration: 2028-12-27

Abstract

The utility model discloses a qualitative/quantitative biochip detection device, characteristics are including the supporting seat, are provided with testing platform on the supporting seat, and testing platform is last to be provided with biochip reaction mechanism and image display mechanism, and biochip reaction mechanism is provided with light source and image capture mechanism in, and image capture mechanism is connected with image display mechanism electricity. The detection device has the advantages that the detection device integrates the biochip reaction mechanism, the image display mechanism, the light source and the image capturing mechanism on one detection platform, wherein the biochip reaction mechanism is used for reacting the biochip, the light source provides the light source for the biochip during detection, then the image capturing mechanism captures image information on the biochip and transmits the captured image information to the image display mechanism, the structure is simple, the portability is good, and a relatively accurate observation result can be obtained; in addition, the supporting seat is used for supporting the detection platform.

Description

Qualitative/quantitative biochip detection device

Technical Field

The utility model relates to a biochip detection area especially relates to a qualitative/quantitative biochip detection device.

Background

The biochip (biochip or bioarray) integrates biochemical analysis process on the chip surface according to the principle of specific interaction between biological molecules, thereby realizing high-flux rapid detection of DNA, RNA, polypeptide, protein and other biological components. The narrow sense of biochip concept refers to a biomolecule lattice formed by immobilizing biomolecules (oligonucleotides, cDNAs, genomic DNAs, polypeptides, antibodies, antigens, etc.) on a solid phase transmitter such as a silicon wafer, a glass plate (beads), a plastic plate (beads), a gel, a nylon membrane, etc. by various methods. The concept of a chip is taken from the concept of integration, a biochip being the integration of biological material. Like laboratory tests, the examination of blood glucose, proteins, enzyme activities, etc. on biochips is based on the same biological reaction principles. Therefore, the biochip is a carrier platform, is a carrier, and is a micro biochemical analysis system which integrates discontinuous analysis processes in the life science field on the surface of a substrate so as to realize accurate, rapid and large-information-quantity detection on cells, proteins, genes and other biological components. The biochip has the technical advantages that the detection is faster, more sensitive and more accurate.

the conventional biochip detection firstly needs to react with a biological sample, and after the reaction is finished, the specific detection and research aiming at the biochip are mainly carried out by two modes, one mode is that the biochip is observed by naked eyes of a human body by means of a microscope, and then the analysis and the research are carried out according to the observed result; the other method is to scan the biochip with a scanner, input the scanning result into a computer, observe the scanning result with the computer, and conduct in-depth research according to the observation result. The two methods mainly have the following problems: in the former, the biochip is observed by naked eyes of a human body by means of a microscope, but the observation result is difficult to record, and a sketch of the observation result can be drawn only by observing while the observation is carried out, which has high requirements on the drawing capability of an observer, so that the detection and research mode is inconvenient to record the observation result, and the accuracy of the observation result is difficult to ensure, thereby causing certain influence on subsequent research; although the latter can obtain a clear observation result by combining the scanner and the computer, the scanner and the computer must coexist in the detection mode, and the scanner and the computer are not necessary, if only can scan the biochip, the scanning result cannot be displayed, if only can the computer can not scan the biochip, the observation result cannot be obtained, and the scanner and the computer are large in size, poor in portability and inconvenient to use. Meanwhile, when a biological sample is reacted, equipment such as a nucleic acid amplification instrument, a chip hybridization instrument, a chip cleaning machine and the like is required, and the whole set of related equipment in the current market is expensive, large and heavy in size, not suitable for frequent movement and difficult to move.

Disclosure of Invention

The utility model aims to solve the technical problem that a qualitative/quantitative biochip detecting system that is small, is convenient for remove and can obtain comparatively accurate observation result is provided.

The utility model provides a technical scheme that above-mentioned technical problem adopted does:

A qualitative/quantitative biochip detection device is characterized by comprising a supporting seat, wherein a detection platform is arranged on the supporting seat, a biochip reaction mechanism and an image display mechanism are arranged on the detection platform, a light source and an image capturing mechanism are arranged in the biochip reaction mechanism, and the image capturing mechanism is electrically connected with the image display mechanism.

The biochip reaction mechanism comprises a reaction cavity arranged on the detection platform, a transparent cavity cover is arranged on the reaction cavity in a turnover way, the reaction cavity is internally provided with a horizontal clapboard which divides the reaction cavity into an upper cavity and a lower cavity, a heating plate is arranged in the upper chamber, a placing seat for placing the biochip is arranged on the horizontal clapboard, the image capturing mechanism is a camera, the horizontal clapboard is provided with a shooting hole which is communicated up and down, the camera is right opposite to the shooting hole and is used for shooting the biological information on the biological chip, the image display mechanism is a display screen arranged on the detection platform, the camera is electrically connected with the display screen, and the light source is arranged on the inner end face of the cavity cover and used for illuminating the upper cavity. The biochip reaction mechanism is simple in structure, the reaction cavity is divided into an upper cavity and a lower cavity by the horizontal partition plate, the upper cavity is used for reaction of the biochip, the lower cavity is used for installation of parts such as a camera and a motor, and the structure is neat; the placing seat is used for placing the biochip, so that the biochip can be stably placed in the upper chamber, and the upper chamber is heated through the heating plate, so that a proper reaction temperature is provided for the reaction of the organisms to be detected on the biochip; the camera is used for shooting the information of the biological sample on the biochip after the reaction is finished, and the image information of the biological sample captured by the camera can be displayed through the display screen; simple structure and convenient operation.

Biochip include a transparent reaction disc, the reaction disc in be radial along the circumference and be provided with a plurality of reaction zones, every reaction zone include from inside to outside liquid pool and the reaction tank that sets gradually, liquid pool and reaction tank between be linked together through a pipe, reaction disc's center and every reaction zone in the center of liquid pool line between the center of reaction tank with the central axis of pipe coincide mutually, reaction disc on be provided with a plurality of sampling holes, every liquid pool on the intercommunication be provided with one the sampling hole, reaction disc on be provided with a plurality of exhaust holes, every reaction tank's lateral part intercommunication be provided with one the exhaust hole. Through a plurality of reaction areas arranged on the reaction disc, the biochip can carry out a plurality of groups of reactions at the same time, the operation efficiency is improved, each reaction area comprises a liquid pool and a reaction pool which are sequentially arranged from inside to outside, the liquid pool is communicated with the reaction pool through a conduit, a sample adding hole is arranged on the liquid pool, a biological sample and a reaction reagent are added into the liquid pool through the sample adding hole, then a rotating force is applied to the reaction disc, the biological sample and the reaction reagent in the liquid pool can enter the reaction pool through the conduit to react under the action of the rotating force, other reagents are added in the process, the reagent is also added into the liquid pool through the sample adding hole, then the reagent enters the reaction pool through the conduit by applying the rotating force to the reaction disc, the structure is simple, the operation is convenient, the pollution of the biological sample in the reagent dripping process is effectively avoided, thereby obtain comparatively accurate experimental data, the setting up of exhaust hole makes biological sample and reaction reagent can enter into the reaction tank smoothly.

a non-return pool for preventing liquid from flowing back to the liquid pool is arranged between the liquid pool and the reaction pool, the guide pipe is divided into a first guide pipe section and a second guide pipe section, the liquid pool and the non-return pool are communicated through the first guide pipe section, the non-return pool and the reaction pool are communicated through the second guide pipe section, the connecting line between the center of the reaction disc and the center of the liquid pool, the center of the reaction pool and the center of the non-return pool in each reaction area is coincided with the central axis of the guide pipe, and the width of the non-return pool is larger than that of the guide pipe. The non-return pool with the structure can effectively prevent liquid in the reaction pool from flowing back to the liquid pool, thereby ensuring normal reaction, and having simple structure and stable use.

The reaction disc is characterized in that a motor is arranged in the placing seat, an output shaft of the motor vertically extends out of the placing seat, an installation column is fixedly connected onto the output shaft, an anti-rotation lug is arranged on the side portion of the installation column in an outward protruding mode, an installation hole matched with the installation column in installation is coaxially arranged in the center of the reaction disc, an anti-rotation groove matched with the anti-rotation lug is arranged in the installation hole, and a placing disc is coaxially arranged at the bottom of the installation column. The motor is used for providing centrifugal force for the reaction disc during reaction and is used for driving the reaction disc to rotate during detection, so that the information of each biological sample on the biochip can be shot by the camera; prevent changeing the lug and preventing the cooperation of changeing the recess, ensure that the reaction disc can follow motor output shaft synchronous rotation, shelve the disc and be used for playing the supporting role to the reaction disc.

The reaction cavity is a cylindrical cavity body matched with the reaction disc, and the inner diameter of the reaction cavity is slightly larger than the outer diameter of the reaction disc.

The lower chamber in be provided with radiator fan, the bottom of lower chamber be provided with a plurality of heat dissipation logical grooves. The heat dissipation through groove is matched with the heat dissipation fan, so that after the reaction is finished, the temperature in the reaction cavity can quickly fall back, and the electric elements arranged in the reaction cavity are protected.

The detection platform is internally provided with a circuit board, the circuit board is provided with a control circuit, the heating plate, the light source, the camera and the display screen are respectively electrically connected with the control circuit, the detection platform is also provided with a USB interface, and the USB interface is electrically connected with the control circuit. The control circuit is used for controlling the rotation of the motor, the heating of the heating plate, the display operation of the display screen, the biological sample information and the like; the setting of USB interface for the image information that shoots through the camera can copy out, carries out further analysis again, also can be used to charge for whole device simultaneously.

The reaction cavity is internally provided with a temperature sensor, a temperature measuring probe of the temperature sensor extends into the upper cavity, and the temperature sensor is electrically connected with the control circuit. The temperature sensor is used for detecting the temperature of the upper chamber, so that the control circuit is driven to control the heating plate to provide a proper reaction temperature.

the detection platform is provided with a rechargeable battery, and the rechargeable battery is electrically connected with the control circuit. The whole device is powered by the rechargeable battery, so that the device is good in portability.

The detection platform is provided with an electric connection interface electrically connected with the control circuit, and the rechargeable battery is provided with an electric connection plug matched with the electric connection interface. The structure enables the rechargeable battery to be electrically connected with the control circuit.

Compared with the prior art, the utility model has the advantages of: the detection device integrates the biochip reaction mechanism, the image display mechanism, the light source and the image capturing mechanism on one detection platform, wherein the biochip reaction mechanism is used for reacting the biochip, the light source provides the light source for the biochip during detection, then the image capturing mechanism captures image information on the biochip and transmits the captured image information to the image display mechanism, the structure is simple, the portability is good, and a relatively accurate observation result can be obtained; in addition, the supporting seat is used for supporting the detection platform.

Drawings

FIG. 1 is a schematic view of the structure of the biochip of the present invention;

FIG. 2 is a schematic view of the overall structure of the present invention;

Fig. 3 is a schematic structural view of the present invention in a state where the cavity cover is opened;

FIG. 4 is a schematic structural view of the biochip of the present invention with the cover open;

Fig. 5 is a schematic cross-sectional view of the present invention in a state where the chamber cover is opened;

Fig. 6 is a schematic sectional view of the detection platform of the present invention in a state where the cavity cover is closed;

Fig. 7 is a schematic structural view of the present invention with the rechargeable battery removed.

Detailed Description

The present invention will be described in further detail with reference to the following embodiments.

As shown in FIGS. 1 to 7, a qualitative/quantitative biochip measuring apparatus comprises a supporting base B1, a measuring platform B2 is disposed on the supporting base B1, a biochip reaction mechanism and an image display mechanism B3 are disposed on the measuring platform B2, a light source B4 and an image capture mechanism B5 are disposed in the biochip reaction mechanism, and the image capture mechanism B5 is electrically connected with the image display mechanism B3.

In this embodiment, the biochip reaction mechanism includes a reaction chamber disposed on the detection platform B2, a transparent chamber cover B7 is rotatably disposed on the reaction chamber, a horizontal partition B61 is disposed in the reaction chamber, the reaction chamber is divided into an upper chamber B62 and a lower chamber B63 by the horizontal partition B61, a heating plate B621 is disposed in the upper chamber B62, a holder B611 for holding the biochip a is disposed on the horizontal partition B61, the image capturing mechanism B5 is a camera, a shooting hole B612 is disposed on the horizontal partition B61, the camera faces the shooting hole B612 for shooting the biological information on the biochip a, the image display mechanism B3 is a display screen disposed on the detection platform B2, the camera is electrically connected to the display screen, and the light source B4 is disposed on the inner end surface of the chamber cover B7 for illuminating the upper chamber B62.

In this embodiment, the biochip a includes a transparent reaction disk a1, a plurality of reaction areas a2 are radially arranged in the reaction disk a1 along the circumference, each reaction area a2 includes a liquid pool a21 and a reaction pool a22 which are sequentially arranged from inside to outside, the liquid pool a21 and the reaction pool a22 are communicated through a conduit, a connecting line between the center of the reaction disk a1 and the center of the liquid pool a21 and the center of the reaction pool a22 in each reaction area a2 coincides with the central axis of the conduit, a plurality of sample adding holes a11 are arranged on the reaction disk a1, one sample adding hole a11 is arranged on each liquid pool a21 in a communication, a plurality of vent holes a12 are arranged on the reaction disk a1, and a vent hole 12 is arranged on the side of each reaction pool a22 in communication.

In this particular embodiment, a check reservoir a23 for preventing liquid from flowing back into liquid reservoir a21 is provided between liquid reservoir a21 and reaction reservoir a22, the conduit is divided into a first conduit segment a24 and a second conduit segment a25, liquid reservoir a21 and check reservoir a23 communicate with each other through first conduit segment a24, check reservoir a23 and reaction reservoir a22 communicate with each other through second conduit segment a25, the line connecting the center of reaction disk a1 with the center of liquid reservoir a21, the center of reaction reservoir a22 and the center of check reservoir a23 in each reaction region a2 coincides with the central axis of the conduit, and the width of check reservoir a23 is greater than the width of the conduit.

in this embodiment, a motor B8 is disposed in the resting seat B611, an output shaft B81 of the motor B8 vertically extends out of the resting seat B611, an installation column B811 is fixedly connected to the output shaft B81, an anti-rotation protrusion B8111 is disposed on an outward protruding side of the installation column B811, an installation hole a13 which is cooperatively installed with the installation column B811 is coaxially disposed at the center of the reaction disk A1, an anti-rotation groove a131 which is engaged with the anti-rotation protrusion B8111 is disposed in the installation hole a13, and a resting disk B812 is coaxially disposed at the bottom of the installation column B811.

In this embodiment, the reaction chamber is a cylindrical chamber body which is matched with the reaction disc A1, and the inner diameter of the reaction chamber is slightly larger than the outer diameter of the reaction disc A1.

In this embodiment, a heat dissipation fan B9 is disposed in the lower chamber B63, and a plurality of heat dissipation through slots B631 are disposed at the bottom of the lower chamber B63.

In this embodiment, a circuit board (not shown) is disposed in the testing platform B2, a control circuit is disposed on the circuit board, the heating plate B621, the light source B4, the camera and the display screen are electrically connected to the control circuit, respectively, a USB interface B201 is further disposed on the testing platform B2, and the USB interface B201 is electrically connected to the control circuit.

In this embodiment, a temperature sensor B601 is disposed in the reaction chamber, a temperature probe of the temperature sensor B601 extends into the upper chamber B62, and the temperature sensor B601 is electrically connected to the control circuit.

In this embodiment, the testing platform B2 is provided with a rechargeable battery B10, and the rechargeable battery B10 is electrically connected to the control circuit.

In this embodiment, the detection platform B2 is provided with an electrical connection interface B202 electrically connected to the control circuit, and the rechargeable battery B10 is provided with an electrical connection plug B101 matching with the electrical connection interface B202.

Claims

1. a qualitative/quantitative biochip detection device is characterized by comprising a supporting seat, wherein a detection platform is arranged on the supporting seat, a biochip reaction mechanism and an image display mechanism are arranged on the detection platform, a light source and an image capturing mechanism are arranged in the biochip reaction mechanism, and the image capturing mechanism is electrically connected with the image display mechanism.

2. the qualitative/quantitative biochip measuring apparatus according to claim 1, wherein the biochip reaction mechanism comprises a reaction chamber disposed on the measuring platform, a transparent cover is rotatably disposed on the reaction chamber, a horizontal partition is disposed in the reaction chamber, the horizontal partition divides the reaction chamber into an upper chamber and a lower chamber, a heating plate is disposed in the upper chamber, a holder for holding the biochip is disposed on the horizontal partition, the image capturing mechanism is a camera, a shooting hole is disposed on the horizontal partition, the camera faces the shooting hole for shooting the biological information on the biochip, the image displaying mechanism is a display screen disposed on the measuring platform, and the camera is electrically connected to the display screen, the light source is arranged on the inner end face of the cavity cover and used for illuminating the upper cavity.

3. The qualitative/quantitative biochip measuring apparatus according to claim 2, wherein the biochip comprises a transparent reaction disk, a plurality of reaction areas are radially arranged in the reaction disc along the circumference, each reaction area comprises a liquid pool and a reaction pool which are sequentially arranged from inside to outside, the liquid pool and the reaction pool are communicated through a conduit, the connecting line between the center of the reaction disc and the center of the liquid pool and the center of the reaction pool in each reaction area is coincident with the central axis of the conduit, the reaction disc is provided with a plurality of sample adding holes, each liquid pool is communicated with one sample adding hole, the reaction disc is provided with a plurality of exhaust holes, and the side part of each reaction tank is communicated with one exhaust hole.

4. the qualitative/quantitative biochip detecting apparatus according to claim 3, wherein a check reservoir for preventing liquid from flowing back to the liquid reservoir is disposed between the liquid reservoir and the reaction reservoir, the conduit is divided into a first conduit segment and a second conduit segment, the liquid reservoir and the check reservoir are communicated through the first conduit segment, the check reservoir and the reaction reservoir are communicated through the second conduit segment, a connecting line between a center of the reaction disk and a center of the liquid reservoir, a connecting line between a center of the reaction reservoir and a center of the check reservoir in each of the reaction regions coincides with a central axis of the conduit, and a width of the check reservoir is greater than a width of the conduit.

5. The device for qualitative/quantitative biochip detection according to claim 3, wherein the resting base is provided with a motor therein, an output shaft of the motor vertically extends out of the resting base, the output shaft is fixedly connected with a mounting post, a lateral portion of the mounting post is provided with an anti-rotation protrusion in a protruding manner, the reaction disc is coaxially provided with a mounting hole at the center thereof, the mounting hole is provided with an anti-rotation groove therein, the anti-rotation groove is matched with the anti-rotation protrusion, and the bottom of the mounting post is coaxially provided with a resting disc.

6. The qualitative/quantitative biochip measuring apparatus according to claim 5, wherein the lower chamber has a heat dissipating fan therein, and the bottom of the lower chamber has a plurality of heat dissipating through-slots.

7. The qualitative/quantitative biochip measuring device according to claim 6, wherein the measuring platform has a circuit board disposed therein, the circuit board has a control circuit disposed thereon, the heating plate, the light source, the camera and the display are electrically connected to the control circuit, the measuring platform further has a USB interface, and the USB interface is electrically connected to the control circuit.

8. The qualitative/quantitative biochip measuring device according to claim 7, wherein a temperature sensor is disposed in the reaction chamber, a temperature probe of the temperature sensor extends into the upper chamber, and the temperature sensor is electrically connected to the control circuit.

9. The qualitative/quantitative biochip detection apparatus of claim 7, wherein the detection platform is provided with a rechargeable battery, and the rechargeable battery is electrically connected to the control circuit.

10. The qualitative/quantitative biochip measuring apparatus according to claim 9, wherein the testing platform has an electrical connection interface electrically connected to the control circuit, and the rechargeable battery has an electrical connection plug engaged with the electrical connection interface.