CN210394386U

CN210394386U - Portable PCR device

Info

Publication number: CN210394386U
Application number: CN201921067824.5U
Authority: CN
Inventors: 张志峰; 王晓飞; 胡文闯
Original assignee: Chengdu Wanzhong One Core Biotechnology Co ltd
Current assignee: Chengdu Wanzhong One Core Biotechnology Co ltd
Priority date: 2019-07-09
Filing date: 2019-07-09
Publication date: 2020-04-24
Anticipated expiration: 2029-07-09

Abstract

The utility model relates to a portable PCR device, be in including power module, casing, the setting that is used for the power supply operation and display module, setting on the casing are in just have the reaction module and the setting of micropore array PCR reaction chip in the casing and be used for right in the casing PCR reaction on the PCR reaction chip provides reaction temperature's control by temperature change execution module. The utility model discloses a PCR device adopts micropore array PCR reaction chip, do not need external liquid drop generater, can be directly with the single mode board sample dispersion by the dilution to the micropore that sets up on the PCR reaction chip, and give this PCR device power supply through power module, realize the PCR reaction of sample and provide reaction temperature to the PCR reaction through PCR reaction chip and control by temperature change execution module, realize the reaction time to this PCR device through operation and display module, the setting and the demonstration of temperature curve and cycle number isoparametric, moreover, the steam generator is simple in structure, easy operation, and low detection cost.

Description

Portable PCR device

Technical Field

The utility model belongs to the technical field of molecular biology, concretely relates to portable PCR device.

Background

Polymerase chain reaction, PCR for short, is a molecular biology technique used to amplify specific DNA fragments, and is one of the most commonly used molecular biology techniques.

A droplet PCR device, one of the common PCR devices at present, is required to perform a micro-droplet treatment on a large amount of diluted nucleic acid sample solution before amplification, so as to disperse a single template sample into a micro-reactor or a droplet of a chip; the micro-titration of the sample needs to use a droplet generator, and the conventional droplet generator is generally complex in structure and large in size, so that the PCR device matched with the droplet generator is not easy to carry and is high in price.

At present, the semiconductor biochip technology with the micropore array is developed rapidly, and in biological and chemical analysis, the consumption of a detection sample and a reagent is very low, so the semiconductor biochip technology with the micropore array has good development prospect in molecular biology, chemistry or clinical diagnosis. For example, chinese patent CN107083326A discloses a microchamber array type digital PCR chip based on PDMS material, which discloses the chip structure, comprising a sample inlet, a microchamber array and a sample outlet, wherein the sample inlet comprises a sample inlet and a water inlet, and the sample outlet comprises a sample outlet and a water outlet; the water sample inlet and the water sample outlet are communicated with a water microchamber array positioned at the periphery of the chip; the sample inlet and the sample outlet are communicated with each reaction chamber in the microchamber array through a microchannel. The chip can be used for PCR reaction.

However, no literature has been reported on the specific structural design of a PCR device for applying a semiconductor biochip having a microwell array to a PCR reaction.

Disclosure of Invention

The present invention is directed to overcoming the disadvantages of the prior art and to providing a portable PCR device.

In order to achieve the above purpose, the utility model adopts the technical scheme that:

the utility model provides a portable PCR device, the PCR device is in including power module, the casing that is used for the power supply, setting operation and display module on the casing, setting are in just have the reaction module of micropore array PCR reaction chip in the casing and set up in the casing and be used for to PCR reaction on the PCR reaction chip provides the control by temperature change execution module of reaction temperature.

Further, the reaction module comprises a hinge assembly for installing the PCR reaction chip, a circuit board arranged in the shell and a reaction control unit arranged on the circuit board, wherein the hinge assembly is arranged above the circuit board, and the reaction control unit is electrically connected with the temperature control execution module to control the reaction temperature provided by the temperature control execution module.

Furthermore, the PCR device also comprises a sealing element used for sealing the micropores on the PCR reaction chip and isolating the micropores from each other, and the hinge assembly comprises a hinge base arranged on the circuit board and used for mounting the PCR reaction chip and a hinge cover, wherein one end of the hinge cover is rotationally connected with one end of the hinge base and used for pressing the sealing element on the PCR reaction chip.

According to an aspect of the utility model discloses an implement, hinge subassembly is still including setting up the other end of hinge lid and be used for with hinge base joint or the couple of separation.

According to the utility model discloses an embodiment, the hinge base includes base body and sets up just be used for the installation on the base body the chip installation piece of PCR reaction chip, the hinge lid with base body rotates to be connected, the couple with base body joint or separation.

According to the utility model discloses an embodiment, hinge lid include one end with hinge base's one end is rotated the hinge lid body of connection and is set up on the hinge lid body and be used for compressing tightly the chip briquetting of PCR reaction chip, the couple sets up the other end of hinge lid body.

Furthermore, the temperature control execution module comprises a heating unit, a heat dissipation unit and a temperature sensor, wherein the heating unit is arranged below the circuit board and in contact with the PCR reaction chip for heat conduction, the heat dissipation unit is arranged below the heating unit and in contact with the heating unit for heat conduction, and the temperature sensor is used for detecting the reaction temperature, and the heating unit, the heat dissipation unit and the temperature sensor are respectively electrically connected with the reaction control unit.

Still further preferably, the heating unit includes a heat conduction block, a heater disposed below the heat conduction block and in contact with the heat conduction block for heat conduction, and a heat conduction block pressing plate disposed above the heater, wherein the heat conduction block is located between the heat conduction block pressing plate and the heater, and the upper end portion of the heat conduction block sequentially penetrates through the heat conduction block pressing plate and the circuit board to be in contact with the PCR reaction chip.

In some embodiments, the upper end surface of the heat conducting block is provided with a groove, and the temperature sensor is arranged in the groove. Preferably, the temperature sensor is a thin film temperature sensor.

Still more preferably, a carbon film is provided between contact surfaces of the PCR reaction chip and the heating unit.

Still further preferably, the heat dissipation unit includes a heat dissipation plate disposed below the heating unit and in contact with the heating unit for heat conduction, a heat conduction cover disposed below the heat dissipation plate and in contact with the heat dissipation plate for heat conduction, and a fan disposed in the heat conduction cover.

According to some implementation aspects of the utility model, the PCR device is still including setting up detection and processing unit on the circuit board, the micropore bottom of PCR reaction chip is provided with biochemical sensor, detection and processing unit is used for gathering biochemical sensor's signal carries out the processing analysis to the signal, then feeds back the analysis result operation and display module.

According to certain embodiments of the present invention, the surface of the biochemical sensor is coated with a hydrogen ion sensitive material, a phosphate ion or a pyrophosphate ion sensitive material.

According to some other embodiments of the present invention, the biochemical sensor is one of an ion sensitive field effect transistor, a nanowire, graphene or molybdenum disulfide transistor sensor, or a miniature electrochemical sensor.

According to some embodiments of the utility model, be equipped with on the hinge base with biochemical sensor pin pad forms electrical connection's probe, the probe with the detection and processing unit is connected in order to gather biochemical sensor's the signal of telecommunication.

Furthermore, the detection processing unit comprises a data acquisition circuit, an ADC chip and a processor, wherein the data acquisition circuit acquires signals of the biochemical sensor at the bottom of the micropore, the ADC chip performs analog-to-digital conversion on the signals, the signals are transmitted to the processor for digital signal processing and analysis, and then analysis results are fed back to the operation and display module.

Furthermore, the micropore array of the PCR reaction chip contains 0.1-1000 ten thousand micropores, and the volume of each micropore is 1 fL-10 pL.

Further, the casing include the upper end have open-ended casing body and with casing body rotates to be connected and is used for the lid to close open-ended casing upper cover, operation and display module set up on the casing upper cover, the PCR device is still including setting up this internal and with the backup pad that casing body upper end is connected of casing, be provided with in the backup pad and be used for the installation reaction module's installation position, reaction module sets up on the installation position, control by temperature change execution module sets up the reaction module below.

Furthermore, the PCR device further comprises tweezers and a vacuum pen, and the support plate is provided with a tweezers placing position and a vacuum pen placing position which are respectively used for placing the tweezers and the vacuum pen.

Furthermore, the power module comprises a power panel arranged in the shell, and a power line interface and a power switch which are respectively arranged on the shell. And a USB interface is also arranged on the shell.

Further, the operation and display module comprises a touch display screen arranged on the shell and a screen control panel arranged between the touch display screen and the shell.

Because of above-mentioned technical scheme's application, compared with the prior art, the utility model have the following advantage:

the utility model discloses a PCR device adopts micropore array PCR reaction chip, do not need external liquid drop generater, can be directly with containing the reaction liquid dispersion of the single mode board sample diluted to the micropore that sets up on the PCR reaction chip, and give this PCR device power supply through power module, realize the PCR reaction of sample and provide reaction temperature to the PCR reaction through PCR reaction chip and control by temperature change execution module, realize the reaction time to this PCR device through operation and display module, the setting and the demonstration of temperature curve and cycle number isoparametric, easy operation, detect low cost.

The PCR device of the utility model does not need an external liquid drop generator, and greatly reduces the reaction system, thereby realizing the miniaturization of the instrument. And the structure is simple, the weight is light, the carrying is convenient, and the price of the instrument is low.

Drawings

FIG. 1 is a schematic perspective view of a portable PCR device according to an embodiment of the present invention;

FIG. 2 is a schematic rear view of a portable PCR device according to an embodiment of the present invention;

FIG. 3 is a schematic bottom view of a portable PCR device according to an embodiment of the present invention;

FIG. 4 is a schematic cross-sectional view of a portable PCR device according to an embodiment of the present invention;

FIG. 5 is an exploded view of a portable PCR device according to an embodiment of the present invention;

FIG. 6 is an exploded view of a hinge assembly and a PCR reaction chip of a portable PCR device according to an embodiment of the present invention;

FIG. 7 is a digital two-dimensional thermodynamic diagram illustrating the PCR device according to an embodiment of the present invention used for detecting the PCR result of a specific PCR chip;

FIG. 8 is a schematic histogram of sensor signals when a PCR device according to an embodiment of the present invention is used for detecting the result of PCR reaction of a specific PCR reaction chip;

in the figure: 1. a PCR reaction chip; 2. a hinge assembly; 201. a base body; 202. a hinge cover body; 203. a chip mounting block; 204. a chip mounting groove; 205. pressing the chip into a block; 206. hooking; 3. a circuit board; 4. a heat conducting block; 5. a heater; 6. a heat conducting block pressing plate; 7. a temperature sensor; 8. a heat sink; 9. a fan; 10. a heat conducting cover; 11. a housing body; 1101. a housing side plate; 1101a, a housing side plate main body portion; 1101b, an interface connection portion; 1102. a housing base; 12. a housing upper cover; 13. a support plate; 14. a power switch; 15. a power panel; 16. touching a display screen; 17. a screen control panel; 18. tweezers; 19. a vacuum pen.

Detailed Description

The invention is further described with reference to the drawings and the specific embodiments.

Referring to fig. 1-6, the portable PCR device of an embodiment of the present invention comprises a power module for supplying power, a housing, an operation and display module disposed on the housing, a reaction module disposed in the housing and having a micro-porous array PCR reaction chip 1, and a temperature control execution module disposed in the housing and used for providing a reaction temperature for the PCR reaction on the PCR reaction chip 1.

The casing includes that the upper end has open-ended casing body 11 and rotates with casing body 11 and be used for covering this open-ended casing upper cover 13, and this PCR device still includes setting up in casing body 11 and the backup pad 13 of being connected with casing body 11 upper end, is provided with the installation position that is used for installing reaction module on the backup pad 13, and control by temperature change execution module sets up in reaction module below.

The reaction module comprises a hinge component 2 for installing the PCR reaction chip 1, a circuit board 3 arranged on the installation position and a reaction control unit arranged on the circuit board 3, the hinge component 2 is arranged above the circuit board 3, and the reaction control unit is electrically connected with the temperature control execution module to control the reaction temperature provided by the temperature control execution module. When casing upper cover 13 lid closed casing body 11, casing body 11 and casing upper cover 13 enclose jointly and enclose to hold the chamber, and backup pad 13 and circuit board 3 will hold the chamber and separate into first chamber and the second of holding and hold the chamber, and temperature control execution module is located the second and holds the chamber, and hinge subassembly 2 is located first chamber that holds.

The PCR device also comprises a sealing member for sealing the micro-wells on the PCR reaction chip and isolating the micro-wells from each other.

Referring to fig. 6, the hinge assembly 2 includes a hinge base disposed on the circuit board 3 and used for mounting the PCR reaction chip, a hinge cover having one end rotatably connected to one end of the hinge base and used for pressing the sealing member onto the PCR reaction chip 1, and a hook 206 disposed at the other end of the hinge cover and used for being engaged with or separated from the hinge base. The hinge base comprises a base body 201 arranged on a circuit board 3, and a chip mounting block 203 arranged on the base body 201 and used for mounting a PCR reaction chip 1, wherein the hinge cover comprises a hinge cover body 202 and a chip pressing block 205, one end of the hinge cover body 202 is rotatably connected with one end of the base body 201, the chip pressing block 205 is arranged on the hinge cover body 202 and used for pressing a sealing element on the PCR reaction chip 1, and a hook 206 is arranged at the other end of the hinge body 202; the chip mounting block 203 is provided with a chip mounting groove 204, and the PCR reaction chip 1 is mounted in the chip mounting groove 204.

The temperature control execution module comprises a heating unit, a heat dissipation unit and a temperature sensor 7, wherein the heating unit is arranged below the circuit board 3 and is in contact with the PCR reaction chip 1 for heat conduction, the heat dissipation unit is arranged below the heating unit and is in contact with the heating unit for heat conduction, the temperature sensor 7 is used for detecting the reaction temperature, and the heating unit, the heat dissipation unit and the temperature sensor 7 are respectively and electrically connected with the reaction control unit. The reaction control unit coordinates the heat dissipation unit and the heating unit to alternately and cooperatively work to provide corresponding temperature for the amplification reaction.

Referring to fig. 5, the heating unit includes a heat conduction block 4, a heater 5 disposed below the heat conduction block 4 and contacting the heat conduction block 4 for heat conduction, and a heat conduction block pressing plate 6 disposed above the heater 5, wherein the heat conduction block 4 is disposed between the heat conduction block pressing plate 6 and the heater 5, and the upper end portion of the heat conduction block 4 sequentially penetrates through the heat conduction block pressing plate 6 and the circuit board 3 to contact the lower surface of the PCR reaction chip 1. The heater 5 can be a semiconductor heater; the upper end surface of the heat conducting block 4 is provided with a groove, and the temperature sensor 7 is arranged in the groove; a carbon film is provided between the contact surfaces of the PCR reaction chip 1 and the heat conductive block 6.

In this example, the heat conduction block 4 includes a plate-like portion located between the heat conduction block holder 6 and the heater 5 and a columnar portion formed on the upper surface of the plate-like portion, and the columnar portion passes through the heat conduction block holder 6, the circuit board 3 and the chip mounting block 203 in this order so that the upper end portion of the columnar portion is in contact with the lower surface of the PCR reaction chip 1. The groove is formed in the upper end face of the columnar portion; the temperature sensor 7 is a thin film temperature sensor; the carbon film is provided between the contact surfaces of the upper end surface of the columnar section and the lower surface of the PCR reaction chip 1.

In this example, the heat dissipating unit includes a heat dissipating fin 8 disposed below the heater 5 and in contact with the heater 5 to conduct heat, a heat conducting cover 10 disposed below the heat dissipating fin 8 and in contact with the heat dissipating fin 8 to conduct heat, and a fan 9 disposed in the heat conducting cover 10.

Referring to fig. 5, the power module includes a power board 15 disposed in the housing, and a power line interface, a power switch 14 and a USB interface respectively disposed on the housing. The power line interface, the power switch 14 and the USB interface are disposed on the housing body 11. In this example, the power board 15 is located in the second accommodation cavity, the case body 11 is composed of a case side plate 1101 and a case chassis 1102 connected to a lower end portion of the case side plate 1101, the case side plate 1101 includes a case side plate body 1101a and an interface connection portion 1101b provided on the case side plate body, and the power line interface, the power switch 14, and the USB interface are provided on the interface connection plate 1101b, respectively.

The operation and display module of the PCR device includes a touch display screen 16 provided on the housing upper cover 12 and a screen control board 17 provided between the touch display screen 16 and the housing upper cover 12.

The PCR device also comprises a pair of tweezers 18 and a vacuum pen 19, and the support plate 13 is provided with a tweezers placing position and a vacuum pen placing position which are respectively used for placing the tweezers 18 and the vacuum pen 19. The tweezers 18 are used for clamping the PCR reaction chip 1, the vacuum pen 19 is used for enabling the sample and the reaction liquid to enter the micropores of the PCR reaction chip 1, and then the micropores are sealed by a sealing member, so that the liquids in the different micropores are isolated from each other, and each micropore has one target calculation molecule, so that the single-molecule PCR amplification of the sample is realized.

In this example, the PCR reaction chip is a microwell array type PCR reaction chip having a microwell array containing 0.1 to 1000 ten thousand microwells, and the volume of each microwell is 1fL to 10 pL. The bottom of each micropore of the PCR reaction chip is also provided with a biochemical sensor.

The biochemical sensor may be the following ones:

such as a biochemical sensor, is coated with a hydrogen ion sensitive material, such as SiO₂、Al₂O₃、HfO₂、TiO₂、Ta₂O₅Or (3-aminopropyl) triethoxysilane, or phosphate ion or pyrophosphate ion sensitive materials, such as phosphate ion organic film ISM or C4- [3 ]]Molecular probes such as rotaxane, cyanostar, and the like. Specifically, the biochemical sensor is an ISFET (ion sensitive field effect transistor) produced by a standard CMOS (complementary metal oxide semiconductor) semiconductor process. A hydrogen ion sensitive material is deposited on a metal floating gate of the ISFET, and the hydrogen ion sensitive material can detect hydrogen ions in the PCR reaction solution or the pH value in the reaction solution.

The biochemical sensor can also adopt a field effect transistor nanowire FET with a new nanowire as a channel or an FET with graphene, molybdenum disulfide and other two-dimensional semiconductor materials as the channel, and compared with an ISFET, the nano-transistor sensors can provide higher sensitivity, thereby providing a smaller reaction cavity and higher integration level and accuracy.

The biochemical sensor can also use conventional electrodes to detect hydrogen ions or phosphate ions, i.e. a miniature electrochemical sensor.

The PCR device also comprises a detection processing unit arranged on the circuit board 3, wherein the detection processing unit is used for collecting signals of the biochemical sensor, processing and analyzing the signals and then feeding back an analysis result to the operation and display module.

The hinge base is provided with a probe which is electrically connected with the biochemical sensor pin pad, and the probe is connected with the detection processing unit to collect an electric signal of the biochemical sensor. Specifically, the probe is provided on the chip mounting block 203.

The detection processing unit comprises a data acquisition circuit, an ADC chip and a processor, wherein the data acquisition circuit acquires an electric signal of the biochemical sensor at the bottom of the micropore, the ADC chip performs digital-to-analog conversion, and then the electric signal is transmitted to the processor for digital signal processing and analysis, and then an analysis result is fed back to the operation and display module. Specifically, the processor is an FPGA, a DSP, or an ARM.

In this example, the operation and display module is also used for performing test operation and result display for detecting the reaction solution in the PCR reaction chip.

The PCR device not only can realize PCR reaction, but also can realize the detection of the reaction result of the PCR reaction.

The working principle of the PCR reaction of the PCR device is as follows: in the process of using the PCR device, the power supply module supplies power to the PCR device, the PCR reaction of a sample and the reaction temperature for the PCR reaction are realized through the PCR reaction chip and the temperature control execution module, and the setting and the display of parameters such as the reaction time, the temperature curve, the cycle number and the like of the PCR device are realized through the operation and display module.

The specific working process of the PCR reaction is as follows: the upper cover 12 and the hinge cover of the shell are opened in sequence, reaction liquid containing diluted single-template samples is directly dripped to the upper surface of the PCR reaction chip by a liquid transfer device, the samples and the reaction liquid enter the micropores of the PCR reaction chip, then the micropores on the PCR reaction chip 1 are sealed by a sealing piece so as to seal a PCR reaction area, the hinge cover is closed, a hook 206 is clamped with the base body 201, a chip pressing block 205 tightly presses the sealing piece on the PCR reaction chip 1, amplification reaction can be started after parameters such as reaction time, a temperature curve and cycle times are set through a touch display screen, the reaction control unit controls the heating unit and the heat dissipation unit to work alternately to provide reaction temperature for reaction in the reaction process, and the reaction is completed after the cycle times reach a set value.

The working principle of the detection of the reaction result of the PCR reaction of the PCR device is as follows: the reaction chip is subjected to pH value test of the reaction solution before the PCR reaction, and then the reaction chip after the PCR reaction is finished is subjected to pH value test of the reaction solution, and data analysis is performed according to the change situation of the secondary pH value, so that the PCR reaction result is obtained.

To better explain the operation principle of the PCR device, a specific PCR reaction chip is used for PCR reaction and detection of the PCR reaction result.

The PCR reaction chip is a micropore array reaction chip taking CMOS ISFET as a biochemical sensor, and the specific indexes are as shown in table 1:

TABLE 1 indices of the microwell array reaction chip

Kind of sensor	ISFET
		Number of microwells and sensors	26 ten thousand
Micropore size (Length, Width, depth)	2 micron, 1.5 micron
		Kind of sensing material on sensor surface	Ta₂O₅
Thickness of sensing material on sensor surface	20nm
		Detection of signal types in PCR	Change in hydrogen ion concentration, or pH

The specific operation steps are as follows:

1. sample loading

The target DNA solution with the concentration of 5pM is evenly mixed with PCR amplification solution containing DNA polymerase, dNTPs and primers. The amount and ratio of the solution are shown in Table 2. And (2) dripping the mixed solution onto a micropore array PCR reaction chip with a biochemical sensor to ensure that each micropore contains the mixed solution, sealing by using a sealing element, and properly diluting the solution to be detected in the sample loading process to ensure that the number of DNA fragments in the DNA solution to be detected is less than or equal to the number of micropores (26 ten thousand) of the chip, so that only one DNA fragment is contained in the micropore as far as possible.

Table 2: preparation of PCR solution

DNA Template	5pM
		PCR MIX	5ul
Primer	Proper amount of
		Deionization of H₂O	To 10ul

2. PCR amplification reaction

Placing the chip obtained in the step 1 into a PCR device for PCR amplification, wherein the amplification conditions are shown in Table 3:

table 3: PCR amplification conditions

3. Signal reading

Before the step 2, the operation and display module selects a program to detect the pH value of the reaction solution in the reaction chip which is not subjected to the PCR reaction. And then, carrying out pH detection on the reaction solution after the PCR reaction, carrying out data analysis processing according to the pH change condition, and feeding back the data to an operation display screen.

In the process of data driving of the chip, 26 thousands of data are generated at each time point and are used as a frame and respectively correspond to 26 thousands of biochemical sensors. As shown in fig. 7 (fig. 7 is an example), the data are arranged in 512 rows × 512 columns to form a two-dimensional thermodynamic diagram, and the color of each pixel corresponds to the magnitude of the output signal of the corresponding sensor, i.e., the pH value in the corresponding microwell. Multiple time points may generate a multi-frame two-dimensional thermodynamic diagram.

The method comprises the following specific steps:

in the detection process, a plurality of frames of two-dimensional thermodynamic diagram data are obtained, a frame of average value thermodynamic diagram is obtained for the obtained data, Histogram analysis is performed on the average value thermodynamic diagram, and as shown in fig. 8, a plurality of distribution peaks are obtained: if the pH value in the micropore without PCR is not changed and the output of the sensor is 0, the corresponding micropore is a negative reaction unit, namely a negative sensor; secondly, if the pH value of the micropores of a single DNA sample subjected to PCR reaction changes, the corresponding micropores are positive reaction units, namely positive sensors; there are also small peaks, the pH changes are larger (the sensor signal is larger), i.e. there are many DNA samples in the microwell that are PCR reacted, and if the solution dilution ratio is appropriate, the number of interfering units is small.

This example ultimately yields a number of positive sensors of 13672. Indicating that PCR reaction of a single DNA sample occurred in 13672 microwells.

With the arrangement, the PCR device has the following advantages:

1) the PCR device is convenient and simple to operate, and can operate the reaction chip and the sample after the upper cover of the shell and the hinge cover are opened in sequence, for example, the reaction liquid containing the sample is dripped on the reaction chip, and the reaction chip is installed or taken out.

2) The PCR device has no special requirements on the shape of the sample when the sample is injected into the reaction chip, such as microdroplet requirements, and a common pipettor is used for injecting the sample.

3) The PCR device has low use cost, and can effectively reduce the consumption of reaction liquid and samples required by detection based on the technology of the micropore array chip matched with the PCR device.

4) The PCR device does not need to be provided with a liquid drop generator, so that the cost of the instrument is reduced, and the PCR device has small structure, lighter weight and convenient carrying.

5) The heat conducting block of the PCR device and the reaction chip are both hard, and when the heat conducting block and the reaction chip are in contact, the problem of untight attachment of the contact surface is inevitable. The carbon film has soft hardness and good heat conduction performance relative to the heat conduction block and the reaction chip, and the carbon film with a certain thickness is covered between the heat conduction block and the reaction chip, so that the joint surface between the heat conduction block and the reaction chip can be in close contact, the heat conduction process is reliable, and the reaction temperature is more controllable; on the other hand, the problem of untight attaching is avoided, so that the heat loss is reduced, and the energy consumption is reduced.

6) In the PCR device, the temperature sensor is arranged on the joint surface of the heat-conducting block and the reaction chip, so that the accuracy of reaction temperature detection is improved.

7) The heat dissipation unit of the PCR device comprises a heat dissipation sheet, a fan and a heat conduction cover, and can effectively improve the cooling speed, thereby shortening the reaction time.

8) The PCR device not only can realize PCR reaction, but also can realize the detection of the PCR reaction result.

9) Compared with the existing detection method adopting fluorescence imaging, the PCR device has the advantages that the steps of photographing and the like are not needed, the operation flow is greatly simplified, the experiment operation time is shortened, and the experiment cost is reduced. Meanwhile, the problem of low sensitivity of the existing detection method is also solved.

The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and to implement the present invention, so as not to limit the protection scope of the present invention, and all equivalent changes or modifications made according to the spirit of the present invention should be covered by the protection scope of the present invention.

Claims

1. A portable PCR device, characterized in that: the PCR device comprises a power module for supplying power, a shell, an operation and display module arranged on the shell, a reaction module arranged in the shell and provided with a micropore array type PCR reaction chip, and a temperature control execution module arranged in the shell and used for providing reaction temperature for PCR reaction on the PCR reaction chip.

2. The portable PCR device of claim 1, wherein: the reaction module comprises a hinge component for installing the PCR reaction chip, a circuit board arranged in the shell and a reaction control unit arranged on the circuit board, wherein the hinge component is arranged above the circuit board, and the reaction control unit is electrically connected with the temperature control execution module to control the reaction temperature provided by the temperature control execution module.

3. The portable PCR device of claim 2, wherein: the PCR device is characterized by further comprising sealing elements used for sealing the micropores on the PCR reaction chip and isolating the micropores from one another, wherein the hinge assembly comprises a hinge base and one end, the hinge base is arranged on the circuit board and used for mounting the PCR reaction chip, the hinge base is rotatably connected with one end of the hinge base, and the hinge cover is used for pressing the sealing elements onto the PCR reaction chip.

4. The portable PCR device of claim 3, wherein: the temperature control execution module comprises a heating unit, a heat dissipation unit and a temperature sensor, wherein the heating unit is arranged below the circuit board and in contact with the PCR reaction chip for heat conduction, the heat dissipation unit is arranged below the heating unit and in contact with the heating unit for heat conduction, the temperature sensor is used for detecting the reaction temperature, and the heating unit, the heat dissipation unit and the temperature sensor are respectively and electrically connected with the reaction control unit.

5. The portable PCR device of claim 4, wherein: the heating unit comprises a heat conduction block, a heater and a heat conduction block pressing plate, wherein the heater is arranged below the heat conduction block and is in contact with the heat conduction block for heat conduction, the heat conduction block pressing plate is arranged above the heater, the heat conduction block is positioned between the heat conduction block pressing plate and the heater, and the upper end part of the heat conduction block sequentially penetrates through the heat conduction block pressing plate and the circuit board to be in contact with the PCR reaction chip.

6. The portable PCR device of claim 4, wherein: and a carbon film is arranged between the contact surfaces of the PCR reaction chip and the heating unit.

7. The portable PCR device of claim 5, wherein: the upper end face of the heat conduction block is provided with a groove, and the temperature sensor is arranged in the groove.

8. The portable PCR device of claim 4, wherein: the heat dissipation unit comprises a heat dissipation sheet arranged below the heating unit and in contact with the heating unit for heat conduction, a heat conduction cover arranged below the heat dissipation sheet and in contact with the heat dissipation sheet for heat conduction, and a fan arranged in the heat conduction cover.

9. The portable PCR device of claim 3, wherein: the PCR device also comprises a detection processing unit arranged on the circuit board, the bottom of the micropore of the PCR reaction chip is provided with a biochemical sensor, the detection processing unit is used for collecting the signal of the biochemical sensor, processing and analyzing the signal, and then feeding back the analysis result to the operation and display module.

10. The portable PCR device of claim 9, wherein: the surface of the biochemical sensor is coated with a hydrogen ion sensitive material and a phosphate ion or pyrophosphate ion sensitive material.

11. The portable PCR device of claim 9, wherein: the biochemical sensor is an ion sensitive field effect transistor, one of nanowire, graphene or molybdenum disulfide transistor sensors, or a miniature electrochemical sensor.

12. The portable PCR device of claim 9, wherein: the hinge base is provided with a probe which is electrically connected with the biochemical sensor pin pad, and the probe is connected with the detection processing unit to collect the electric signal of the biochemical sensor.

13. The portable PCR device of claim 12, wherein: the detection processing unit comprises a data acquisition circuit, an ADC chip and a processor, wherein the data acquisition circuit acquires signals of the biochemical sensor at the bottom of the micropore, the ADC chip performs analog-to-digital conversion on the signals, the signals are transmitted to the processor for digital signal processing and analysis, and then analysis results are fed back to the operation and display module.

14. The portable PCR device according to any one of claims 1 to 13, wherein: the micropore array of the PCR reaction chip contains 0.1-1000 ten thousand micropores, and the volume of each micropore is 1 fL-10 pL.

15. The portable PCR device according to any one of claims 1 to 13, wherein: the casing include the upper end have open-ended casing body and with casing body rotates to be connected and is used for the lid to close open-ended casing upper cover, operation and display module set up cover on the casing, the PCR device is still including setting up this internal and with the backup pad that casing body upper end is connected of casing, be provided with in the backup pad and be used for the installation reaction module's installation position, reaction module sets up on the installation position, control by temperature change execution module sets up the reaction module below.

16. The portable PCR device of claim 15, wherein: the PCR device further comprises tweezers and a vacuum pen, and the supporting plate is provided with a tweezers placing position and a vacuum pen placing position, wherein the tweezers placing position and the vacuum pen placing position are respectively used for placing the tweezers and the vacuum pen.

17. The portable PCR device according to any one of claims 1 to 13, wherein: the power module comprises a power panel arranged in the shell, and a power line interface and a power switch which are respectively arranged on the shell.

18. The portable PCR device according to any one of claims 1 to 13, wherein: the operation and display module comprises a touch display screen arranged on the shell and a screen control panel arranged between the touch display screen and the shell.