CN209784378U

CN209784378U - Qualitative/quantitative biochip reaction instrument

Info

Publication number: CN209784378U
Application number: CN201822213416.8U
Authority: CN
Inventors: 周君; 苏秀榕; 芦晨阳; 李妍妍
Original assignee: Ningbo University
Current assignee: Zhejiang Zhenghegu Biotechnology Co.,Ltd.
Priority date: 2018-12-27
Filing date: 2018-12-27
Publication date: 2019-12-13
Anticipated expiration: 2028-12-27

Abstract

The utility model discloses a qualitative/quantitative biochip reactor, characteristics are including reactor body and biochip, and the reactor body includes the reaction box body and can set up the lid on the reaction box body with overturning, are provided with a reaction chamber in the reaction box body, and the reaction intracavity is provided with a horizontal baffle, and horizontal baffle separates the reaction chamber for last cavity and cavity down, and the lid closes to set up on last cavity, goes up the indoor heating mechanism that is used for heating for the reaction chamber that is provided with, is provided with the seat of shelving that is used for shelving biochip on the horizontal baffle. The device has the advantages that the placing seat is used for placing the biochip, so that the biochip can be stably placed in the upper chamber, the heating mechanism heats the upper chamber, and a proper reaction temperature is provided for the reaction of the organisms to be detected on the biochip, and the device is simple in structure and good in portability.

Description

qualitative/quantitative biochip reaction instrument

Technical Field

The utility model relates to a biochip detection area especially relates to a qualitative/quantitative biochip reactor.

Background

The biochip (biochip or bioarray) integrates biochemical analysis process on the chip surface according to the principle of specific interaction between biological molecules, thereby realizing high-flux rapid detection of DNA, RNA, polypeptide, protein and other biological components. The narrow sense of biochip concept refers to a biomolecule lattice formed by immobilizing biomolecules (oligonucleotides, cDNAs, genomicDNAs, polypeptides, antibodies, antigens, etc.) on a solid phase transmitter such as a silicon wafer, a glass plate (beads), a plastic plate (beads), a gel, a nylon membrane, etc. by various methods. The concept of a chip is taken from the concept of integration, a biochip being the integration of biological material. Like laboratory tests, the examination of blood glucose, proteins, enzyme activities, etc. on biochips is based on the same biological reaction principles. Therefore, the biochip is a carrier platform, is a carrier, and is a micro biochemical analysis system which integrates discontinuous analysis processes in the life science field on the surface of a substrate so as to realize accurate, rapid and large-information-quantity detection on cells, proteins, genes and other biological components. The biochip has the technical advantages that the detection is faster, more sensitive and more accurate.

At present, when a biological sample is reacted, equipment such as a nucleic acid amplification instrument, a chip hybridization instrument, a chip cleaning machine and the like is generally required to be used, and the whole set of relevant equipment on the market is expensive, large in size, heavy, not suitable for frequent movement and difficult to move.

Disclosure of Invention

The utility model aims to solve the technical problem of providing a qualitative/quantitative biochip reaction instrument with simple structure and good portability.

the utility model provides a technical scheme that above-mentioned technical problem adopted does:

The utility model provides a qualitative/quantitative biochip reaction appearance, includes reaction appearance body and biochip, the reaction appearance body including the reaction box body with can overturn and set up the reaction box body on the lid, the reaction box body in be provided with a reaction chamber, the reaction intracavity be provided with a horizontal baffle, horizontal baffle will the reaction chamber separate for last cavity and cavity down, the lid close the setting and be in last cavity on, last cavity in be provided with be used for the heating mechanism of reaction chamber heating, horizontal baffle on be provided with and be used for shelving the seat of shelving of biochip.

biochip include a transparent reaction disc, the reaction disc in be radial along the circumference and be provided with a plurality of reaction zones, every reaction zone include from inside to outside liquid pool and the reaction tank that sets gradually, liquid pool and reaction tank between be linked together through a pipe, reaction disc's center and every reaction zone in the center of liquid pool line between the center of reaction tank with the central axis of pipe coincide mutually, reaction disc on be provided with a plurality of sampling holes, every liquid pool on the intercommunication be provided with one the sampling hole, reaction disc on be provided with a plurality of exhaust holes, every reaction tank's lateral part intercommunication be provided with one the exhaust hole. Through a plurality of reaction areas arranged on the reaction disc, the biochip can carry out a plurality of groups of reactions at the same time, the operation efficiency is improved, each reaction area comprises a liquid pool and a reaction pool which are sequentially arranged from inside to outside, the liquid pool is communicated with the reaction pool through a conduit, a sample adding hole is arranged on the liquid pool, a biological sample and a reaction reagent are added into the liquid pool through the sample adding hole, then a rotating force is applied to the reaction disc, the biological sample and the reaction reagent in the liquid pool can enter the reaction pool through the conduit to react under the action of the rotating force, other reagents are added in the process, the reagent is also added into the liquid pool through the sample adding hole, then the reagent enters the reaction pool through the conduit by applying the rotating force to the reaction disc, the structure is simple, the operation is convenient, the pollution of the biological sample in the reagent dripping process is effectively avoided, thereby obtain comparatively accurate experimental data, the setting up of exhaust hole makes biological sample and reaction reagent can enter into the reaction tank smoothly.

A non-return pool for preventing liquid from flowing back to the liquid pool is arranged between the liquid pool and the reaction pool, the guide pipe is divided into a first guide pipe section and a second guide pipe section, the liquid pool and the non-return pool are communicated through the first guide pipe section, the non-return pool and the reaction pool are communicated through the second guide pipe section, the connecting line between the center of the reaction disc and the center of the liquid pool, the center of the reaction pool and the center of the non-return pool in each reaction area is coincided with the central axis of the guide pipe, and the width of the non-return pool is larger than that of the guide pipe. The non-return pool with the structure can effectively prevent liquid in the reaction pool from flowing back to the liquid pool, thereby ensuring normal reaction, and having simple structure and stable use.

The reaction disc is characterized in that a motor is arranged in the placing seat, an output shaft of the motor vertically extends out of the placing seat, an installation column is fixedly connected onto the output shaft, an anti-rotation lug is arranged on the side portion of the installation column in an outward protruding mode, an installation hole matched with the installation column in installation is coaxially arranged in the center of the reaction disc, an anti-rotation groove matched with the anti-rotation lug is arranged in the installation hole, and a placing disc is coaxially arranged at the bottom of the installation column. The motor is used for providing centrifugal force for the reaction disc, prevents changeing the lug and preventing the cooperation of changeing the recess, ensures that the reaction disc can follow motor output shaft synchronous rotation, shelves the disc and is used for playing the supporting role to the reaction disc.

The reaction cavity is a cylindrical cavity body matched with the reaction disc, and the inner diameter of the reaction cavity is slightly larger than the outer diameter of the reaction disc.

The heating mechanism comprises a heating plate, and the heating plate is arranged in the upper chamber. The heating mechanism adopts a heating plate, has simple structure and convenient installation and is used for providing the ambient temperature for reaction.

The heating plate is arranged on the horizontal partition plate and is arranged outside the placing seat in a surrounding manner. Arranged in this position better provides the reaction disc with the temperature required for the reaction.

The device comprises a lower cavity, a heating plate, a reaction cavity, a control circuit board, a motor, a heating plate and a temperature sensor, wherein the lower cavity is internally provided with the control circuit board, the control circuit board is provided with the control circuit, the motor and the heating plate are electrically connected with the control circuit, the reaction cavity is internally provided with the temperature sensor, the temperature measuring probe of the temperature sensor extends into the upper cavity, and the temperature sensor is electrically connected with the control circuit. The control circuit is used for controlling the rotation of the motor, the heating of the heating plate and the like; the temperature sensor is used for detecting the temperature of the upper chamber, so that the control circuit is driven to control the heating plate to provide a proper reaction temperature.

The lower end closing cap of lid be provided with the heating panel that has a plurality of heat dissipation logical grooves, the heating panel with the lid between enclose and establish into a heat dissipation chamber, heat dissipation intracavity be provided with radiator fan, radiator fan with control circuit electricity connect, the lid on be provided with a plurality of first louvres that link up from top to bottom, the bottom of cavity down be provided with a plurality of second louvres that link up from top to bottom. The heat dissipation through groove and the first heat dissipation hole form a heat dissipation channel, and the arrangement of the heat dissipation fan is matched, so that the temperature in the reaction cavity can quickly fall back after the reaction is finished, and the electric elements arranged in the reaction cavity are protected.

The reaction box body is provided with an operation display screen which is electrically connected with the control circuit. The operation display screen is convenient for sending heating or rotating instruction operation to the control circuit.

The one end of lid rotationally set up the reaction box body on, the other end of lid be provided with the locking groove, the corresponding position of reaction box body on be provided with locking groove matched with locking and detain. Through the cooperation of locking groove and locking knot for the lid can be stably closed on reaction box body.

Compared with the prior art, the utility model has the advantages of: the placing seat is used for placing the biochip, so that the biochip can be stably placed in the upper chamber, the heating mechanism heats the upper chamber, a proper reaction temperature is provided for the reaction of the organisms to be detected on the biochip, and the placing seat is simple in structure and good in portability.

Drawings

Fig. 1 is a schematic overall structure diagram of a first embodiment of the present invention;

FIG. 2 is a schematic view of the biochip of the embodiment of the present invention with the cover removed;

FIG. 3 is a schematic view of the structure of the biochip of the present invention;

FIG. 4 is a schematic cross-sectional view of a biochip according to an embodiment of the present invention with the cover removed;

FIG. 5 is a schematic view of a structure of the present invention with a biochip placed thereon and a cover opened;

Fig. 6 is a schematic overall structure diagram of a second embodiment of the present invention;

fig. 7 is a schematic structural view of the second cover in an opened state according to an embodiment of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the following embodiments.

The first embodiment is as follows: as shown in fig. 1 to 5, a qualitative/quantitative biochip reactor comprises a reactor body B and a biochip a, wherein the reactor body B comprises a reaction cartridge B1 and a cover B2 which is reversibly disposed on a reaction cartridge B1, a reaction chamber is disposed in the reaction cartridge B1, a horizontal partition B11 is disposed in the reaction chamber, the reaction chamber is divided into an upper chamber B111 and a lower chamber B112 by the horizontal partition B11, the cover B2 is covered and disposed on the upper chamber B111, a heating mechanism for heating the reaction chamber is disposed in the upper chamber B111, and a holder B12 for holding the biochip a is disposed on the horizontal partition B11.

In this embodiment, the biochip a includes a transparent reaction disk a1, a plurality of reaction areas a2 are radially arranged in the reaction disk a1 along the circumference, each reaction area a2 includes a liquid pool a21 and a reaction pool a22 which are sequentially arranged from inside to outside, the liquid pool a21 and the reaction pool a22 are communicated through a conduit, a connecting line between the center of the reaction disk a1 and the center of the liquid pool a21 and the center of the reaction pool a22 in each reaction area a2 coincides with the central axis of the conduit, a plurality of sample adding holes a11 are arranged on the reaction disk a1, one sample adding hole a11 is arranged on each liquid pool a21 in a communication, a plurality of vent holes a12 are arranged on the reaction disk a1, and a vent hole 12 is arranged on the side of each reaction pool a22 in communication.

In this particular embodiment, a check reservoir a23 for preventing liquid from flowing back into liquid reservoir a21 is provided between liquid reservoir a21 and reaction reservoir a22, the conduit is divided into a first conduit segment a24 and a second conduit segment a25, liquid reservoir a21 and check reservoir a23 communicate with each other through first conduit segment a24, check reservoir a23 and reaction reservoir a22 communicate with each other through second conduit segment a25, the line connecting the center of reaction disk a1 with the center of liquid reservoir a21, the center of reaction reservoir a22 and the center of check reservoir a23 in each reaction region a2 coincides with the central axis of the conduit, and the width of check reservoir a23 is greater than the width of the conduit.

In this embodiment, a motor B13 is disposed in the resting seat B12, an output shaft B131 of the motor B13 vertically extends out of the resting seat B12, an installation column B132 is fixedly connected to the output shaft B131, an anti-rotation protrusion B1321 is disposed on an outward protrusion of a side portion of the installation column B132, an installation hole a13 which is installed in cooperation with the installation column B132 is coaxially disposed in the center of the reaction disk A1, an anti-rotation groove a131 which is matched with the anti-rotation protrusion B1321 is disposed in the installation hole a13, and a resting disk B133 is coaxially disposed at the bottom of the installation column B132.

In this embodiment, the reaction chamber is a cylindrical chamber body which is matched with the reaction disc A1, and the inner diameter of the reaction chamber is slightly larger than the outer diameter of the reaction disc A1.

In this embodiment, the heating mechanism includes a heating plate B14, and a heating plate B14 is disposed within the upper chamber B111.

in this embodiment, the heating plate 4B is disposed on the horizontal partition B11 and is circumferentially disposed outside the resting seat B12.

In this embodiment, a control circuit board B1121 is disposed in the lower chamber B112, a control circuit (not shown) is disposed on the control circuit board B1121, the motor B13 and the heating plate B14 are electrically connected to the control circuit, a temperature sensor B15 is further disposed in the reaction chamber, a temperature probe B151 of the temperature sensor B15 extends into the upper chamber B111, and the temperature sensor B15 is electrically connected to the control circuit.

In this embodiment, the cover B2 has a heat dissipating plate B21 with a plurality of heat dissipating through slots on its bottom cover, a heat dissipating chamber B22 is enclosed between the heat dissipating plate B21 and the cover B2, a heat dissipating fan B23 is disposed in the heat dissipating chamber B22, the heat dissipating fan B23 is electrically connected to the control circuit, a plurality of first heat dissipating holes B24 are disposed on the cover B2, and a plurality of second heat dissipating holes B1122 are disposed on the bottom of the lower chamber B112.

In this embodiment, one end of the cover B2 is rotatably disposed on the reaction cassette B1, the other end of the cover B2 is disposed with a locking groove B25, and a locking button B3 is disposed at a position corresponding to the reaction cassette B1 and engaged with the locking groove B25.

Example two: as shown in fig. 6 and 7, the other parts are the same as those of the first embodiment, except that an operation display panel B101 is disposed on the reaction box body, and the operation display panel B101 is electrically connected to the control circuit.

Claims

1. The utility model provides a qualitative/quantitative biochip reaction appearance, its characterized in that includes reactor body and biochip, the reactor body including the reaction box body with can overturn and set up the reaction box body on the lid, the reaction box body in be provided with a reaction chamber, the reaction intracavity be provided with a horizontal baffle, horizontal baffle will the reaction chamber separate for last cavity and cavity down, the lid close the setting and be in last cavity on, the last cavity in be provided with be used for the heating mechanism of reaction chamber heating, horizontal baffle on be provided with and be used for shelving the seat of shelving of biochip.

2. The qualitative/quantitative biochip according to claim 1, wherein the biochip comprises a transparent reaction disk, a plurality of reaction areas are radially arranged in the reaction disc along the circumference, each reaction area comprises a liquid pool and a reaction pool which are sequentially arranged from inside to outside, the liquid pool and the reaction pool are communicated through a conduit, the connecting line between the center of the reaction disc and the center of the liquid pool and the center of the reaction pool in each reaction area is coincident with the central axis of the conduit, the reaction disc is provided with a plurality of sample adding holes, each liquid pool is communicated with one sample adding hole, the reaction disc is provided with a plurality of exhaust holes, and the side part of each reaction tank is communicated with one exhaust hole.

3. A qualitative/quantitative biochip reactor as defined in claim 2, wherein a check tank for preventing liquid from flowing back to said liquid tank is provided between said liquid tank and said reaction tank, said conduit is divided into a first conduit section and a second conduit section, said liquid tank and said check tank are communicated with each other through said first conduit section, said check tank and said reaction tank are communicated with each other through said second conduit section, a line connecting a center of said reaction disk with a center of said liquid tank, a center of said reaction tank and a center of said check tank in each of said reaction regions coincides with a central axis of said conduit, and a width of said check tank is larger than a width of said conduit.

4. The qualitative/quantitative biochip reaction apparatus according to claim 2, wherein the resting base is provided with a motor therein, an output shaft of the motor vertically extends out of the resting base, the output shaft is fixedly connected with a mounting post, a lateral portion of the mounting post is provided with an anti-rotation protrusion in a protruding manner, a mounting hole is coaxially formed in the center of the reaction disk and is matched with the mounting post, an anti-rotation groove is formed in the mounting hole and is matched with the anti-rotation protrusion, and a resting disk is coaxially formed at the bottom of the mounting post.

5. The qualitative/quantitative biochip reactor according to claim 2, wherein the reaction chamber is a cylindrical chamber body fitted with the reaction disk, and the inner diameter of the reaction chamber is slightly larger than the outer diameter of the reaction disk.

6. The qualitative/quantitative biochip reactor according to claim 4, wherein the heating mechanism comprises a heating plate disposed on the horizontal partition and surrounding the outside of the resting base.

7. The qualitative/quantitative biochip reactor according to claim 6, wherein the lower chamber is provided with a control circuit board, the control circuit board is provided with a control circuit, the motor and the heating plate are both electrically connected to the control circuit, the reaction chamber is further provided with a temperature sensor, a temperature probe of the temperature sensor is inserted into the upper chamber, and the temperature sensor is electrically connected to the control circuit.

8. the qualitative/quantitative biochip reactor according to claim 7, wherein the cover has a heat sink with a plurality of heat dissipating through slots on its bottom cover, a heat dissipating cavity is defined between the heat sink and the cover, a heat dissipating fan is disposed in the heat dissipating cavity and electrically connected to the control circuit, the cover has a plurality of first heat dissipating holes penetrating therethrough, and the bottom of the lower chamber has a plurality of second heat dissipating holes penetrating therethrough.

9. The qualitative/quantitative biochip reactor according to claim 8, wherein the reaction cassette is provided with an operation display screen, and the operation display screen is electrically connected to the control circuit.

10. The qualitative/quantitative biochip reactor according to claim 1, wherein one end of the cover is rotatably disposed on the reaction cassette, the other end of the cover is provided with a locking groove, and a locking buckle is disposed at a position corresponding to the reaction cassette and engaged with the locking groove.