CN102262090B - Method and equipment for measuring platelet aggregation and blood coagulation factor - Google Patents
Method and equipment for measuring platelet aggregation and blood coagulation factor Download PDFInfo
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- CN102262090B CN102262090B CN2010101808065A CN201010180806A CN102262090B CN 102262090 B CN102262090 B CN 102262090B CN 2010101808065 A CN2010101808065 A CN 2010101808065A CN 201010180806 A CN201010180806 A CN 201010180806A CN 102262090 B CN102262090 B CN 102262090B
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Abstract
The invention relates to a method for measuring the platelet aggregation and a blood coagulation factor. In the method, a full-automatic coagulate blood analytical instrument is used, the platelet aggregation is measured by using photoelectric turbidimetry and the blood coagulation factor is measured by using a double magnetic circuit and bead method. The method comprises the following measuring steps of: preparing a platelet aggregation inductive agent, platelet rich plasma and platelet poor plasma; respectively reading light transmittance data of a platelet poor plasma sample and light transmittance data of a platelet rich plasma sample as well as light transmittance data of the platelet rich plasma during the forming process of the platelet aggregation within the specified time; calculating the platelet aggregation rate of the measured blood sample; absorbing the platelet poor plasma and injecting the platelet poor plasma in a hemagglutination cup; absorbing the selected reagent according to a test program and injecting the selected reagent into the hemagglutination cup; automatically monitoring the amplitude of a steel bead; and generating a test report by using measuring results of the platelet aggregation and the blood coagulation factor according to input information of patients.
Description
Technical field
The present invention relates to the assay method and the equipment of a kind of platelet aggregation property and clotting factor; This method is used a kind of automatic coagulation analyzer; Platelet aggregation property is measured according to photoelectric turbidimetry; Clotting factor is measured according to the double magnetic circuit paramagnetic particle method; Said automatic coagulation analyzer comprises conveyer, blood gathering sample needle and needle cleaning device, blood coagulation blood sample pin and the clotting reagent needle device of platelet aggregation proving installation, blood coagulation proving installation, sample disc device, reagent bottle device, cleaning disc device, blood clotting cup; Said blood is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin and is connected with automatic application of sample pipeline, and said cleaning needle is connected with automatic detergent line.
Background technology
Chinese patent 90207370.2 discloses intelligent platelet aggregation instrument, comprises that complete machine is made up of power supply, temperature-adjusting circuit, photoelectric switching circuit, amplifying circuit, programmable amplifying circuit, analog to digital conversion circuit, data converting circuit, imput output circuit, display, keyboard, printer, stirring motor and basic calculating system.Make central processing unit by 8-bit microprocessor, the control each several part has functions such as the automatic zero set (AZS) of test blood plasma, stirring, reporting printing and demonstration time.Adopt the micro computer special circuit that various corresponding control programs are solidificated in the integrated circuit, solve automatic zero set (AZS), different specimen blood plasma is carried out automatic zero set (AZS); Automatically drive stirring motor, and gathering curve and the laboratory report that can will test are printed automatically, are duplicated and show the time at that time automatically.But there is big defective in the method for testing of this patent; Once only can measure a blood sample; The category that belongs to hand dipping not only can cause the prolongation of test period, also can be because of the negligence of operation of manual work; Influence precision of test result, operating personnel are because of directly contacting contaminated with patient's blood sample.Therefore, need to propose the assay method and the equipment of a kind of platelet aggregation property and clotting factor.
Summary of the invention
The object of the present invention is to provide the assay method and the equipment of a kind of platelet aggregation property and clotting factor; This method is used a kind of automatic coagulation analyzer; Platelet aggregation property is measured according to photoelectric turbidimetry; Clotting factor is measured according to the double magnetic circuit paramagnetic particle method, and said automatic coagulation analyzer comprises conveyer, blood gathering sample needle and needle cleaning device, blood coagulation blood sample pin and the clotting reagent needle device of platelet aggregation proving installation, blood coagulation proving installation, sample disc device, reagent bottle device, cleaning disc device, blood clotting cup; Full-automatic detection mode of the present invention; Operating personnel have been avoided because of directly contacting contaminated with patient's blood sample; Protected greatly that the operator's is healthy; All application of sample program, test procedure, cleaning procedure are accomplished by automatic coagulation analyzer automatically, have improved the test speed and the accuracy of clinical examination greatly.
The objective of the invention is to realize: the assay method of a kind of platelet aggregation property and clotting factor by following technical proposals; Use a kind of automatic coagulation analyzer; Said automatic coagulation analyzer comprises conveyer, blood gathering sample needle and needle cleaning device, blood coagulation blood sample pin and the clotting reagent needle device of platelet aggregation proving installation, blood coagulation proving installation, sample disc device, reagent bottle device, cleaning disc device, blood clotting cup; Said blood is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin and is connected with automatic application of sample pipeline, and said cleaning needle is connected with automatic detergent line;
Platelet aggregation property is measured according to photoelectric turbidimetry, and clotting factor is measured according to the double magnetic circuit paramagnetic particle method, and determination step is:
A, collection blood samples of patients sample carry out anti-freezing to this blood sample and handle, and generate anticoagulated whole blood, with the anticoagulated whole blood centrifugal treating, and preparation platelet rich plasma and platelet poor plasma;
B, platelet rich plasma for preparing and platelet poor plasma are placed in the sample disc device, sample number is set;
C, with the reagent bottle device that the platelet aggregation derivant for preparing and clotting reagent are put into automatic coagulation analyzer, cryopreservation is subsequent use;
D, order blood are assembled sample needle and from said sample disc device, are pressed examination criteria consumption absorption platelet poor plasma; Inject the test cup of platelet aggregation proving installation, the platelet aggregation proving installation reads platelet poor plasma sample transmission data and storage automatically; Automatically platelet poor plasma is discharged from test cup bottom then, clean the test cup automatically;
E, order blood are assembled sample needle and from said sample disc device, are pressed examination criteria consumption absorption platelet rich plasma; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads platelet rich plasma sample transmission data and storage automatically, cleans blood then automatically and assembles sample needle;
F, order blood are assembled sample needle and from the reagent bottle device, are pressed examination criteria consumption absorption platelet aggregation derivant; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads the platelet rich plasma sample transmission data in the platelet aggregation forming process in the stipulated time automatically; Describe to assemble curve and storage, automatically the platelet rich plasma sample is discharged from test cup bottom then, clean blood then automatically and assemble sample needle and test cup;
The blood clotting cup that the conveyer of G, blood clotting cup will contain steel ball is delivered on the preheating position in storehouse of blood coagulation proving installation, and application software is discerned sample number;
H, order blood coagulation blood sample pin are pressed the platelet poor plasma that the examination criteria consumption is drawn same patient from said sample disc device, inject the blood clotting cup, automatically the preheating platelet poor plasma;
The conveyer of I, blood clotting cup is delivered to the blood clotting cup on the detection position in storehouse of blood coagulation proving installation; Drive steel ball continuous vibration at the bottom of the blood clotting cup; From the reagent bottle device, press the examination criteria consumption according to the mensuration project order clotting reagent pin of selecting and draw the reagent of selecting, inject selected blood clotting cup;
J, blood coagulation proving installation read the amplitude variations of steel ball automatically, confirm solidifying point, and the clotting factor data of measuring are stored, and the conveyer of blood clotting cup abandons used blood clotting cup;
K, calculate the platelet aggregation rate of tested blood sample, with the mensuration result of platelet aggregation property and clotting factor according to the patient information generation test report of being imported.
The object of the invention can also be realized by following technical proposals: the assay method of a kind of platelet aggregation property and clotting factor; Use a kind of automatic coagulation analyzer; Said automatic coagulation analyzer comprises conveyer, blood gathering sample needle and needle cleaning device, blood coagulation blood sample pin and the clotting reagent needle device of platelet aggregation proving installation, blood coagulation proving installation, sample disc device, reagent bottle device, cleaning disc device, blood clotting cup; Said blood is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin and is connected with automatic application of sample pipeline, and said cleaning needle is connected with automatic detergent line;
Clotting factor is measured according to the double magnetic circuit paramagnetic particle method, and platelet aggregation property is measured according to photoelectric turbidimetry, and determination step is:
A, collection blood samples of patients sample carry out anti-freezing to this blood sample and handle, and generate anticoagulated whole blood, with the anticoagulated whole blood centrifugal treating, and preparation platelet rich plasma and platelet poor plasma;
B, platelet rich plasma for preparing and platelet poor plasma are placed in the sample disc device, sample number is set;
C, with the reagent bottle device that the platelet aggregation derivant for preparing and clotting reagent are put into automatic coagulation analyzer, cryopreservation is subsequent use;
The blood clotting cup that the conveyer of D, blood clotting cup will contain steel ball is delivered on the preheating position in storehouse of blood coagulation proving installation; Order blood coagulation blood sample pin is pressed the examination criteria consumption and is drawn platelet poor plasma from said sample disc device; Inject the blood clotting cup, automatically the preheating platelet poor plasma;
The conveyer of E, blood clotting cup is delivered to the blood clotting cup on the detection position in storehouse of blood coagulation proving installation; Drive steel ball continuous vibration at the bottom of the blood clotting cup; From the reagent bottle device, press the examination criteria consumption according to the mensuration project order clotting reagent pin of selecting and draw the reagent of selecting, inject selected blood clotting cup;
F, blood coagulation proving installation read the amplitude variations of steel ball automatically, confirm solidifying point, and the clotting factor data of measuring are stored, and the conveyer of blood clotting cup abandons used blood clotting cup;
G, application software are discerned sample number; Order blood is assembled sample needle and from said sample disc device, is pressed the platelet poor plasma that the examination criteria consumption is drawn same patient; Inject the test cup of platelet aggregation proving installation, the platelet aggregation proving installation reads platelet poor plasma sample transmission data and storage automatically; Automatically platelet poor plasma is discharged from test cup bottom then, clean the test cup automatically;
H, order blood are assembled sample needle and from said sample disc device, are pressed the platelet rich plasma that the examination criteria consumption is drawn same patient; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads platelet rich plasma sample transmission data and storage automatically, cleans blood then automatically and assembles sample needle;
I, order blood are assembled sample needle and from the reagent bottle device, are pressed examination criteria consumption absorption platelet aggregation derivant; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads the platelet rich plasma sample transmission data in the platelet aggregation forming process in the stipulated time automatically; Describe to assemble curve and storage, automatically the platelet rich plasma sample is discharged from test cup bottom then, clean blood then automatically and assemble sample needle and test cup;
J, calculate the platelet aggregation rate of tested blood sample, with the mensuration result of platelet aggregation property and clotting factor according to the patient information generation test report of being imported.
Another object of the present invention is realized by following technical proposals: a kind of automatic coagulation analyzer; A support is arranged; Support is provided with automatic control circuit and matcher, and platelet aggregation proving installation, sample disc device, cleaning disc device, reagent bottle device, blood coagulation proving installation are set on the testing stand of said support; Be provided with a plurality of test cups in the said platelet aggregation proving installation, the detection light path system be set, comprise the lighting transistor circuit and the photoelectric switching circuit of complete configuration in this detection light path system around this test cup; Below the said test cup magnetic stirring apparatus is set; On the said sample disc device a plurality of sample cups are set; Comprise cleaning fluid bottle and waste collection bottle in the said cleaning disc device; On the said reagent bottle device low temperature holding device is set; Said blood coagulation proving installation middle part is provided with a plurality of tactic preheating positions in storehouse and detects position in storehouse, and said detection position in storehouse symmetria bilateralis is provided with two groups of drive coils, under the said detection position in storehouse measurement coil is set; Said testing stand top is provided with blood and assembles sample needle device, needle cleaning device, blood coagulation blood sample needle device, clotting reagent needle device; Said blood is assembled sample needle device, needle cleaning device, blood coagulation blood sample needle device, clotting reagent needle device and is installed on the running gear, this running gear comprise vertical travel mechanism, laterally travel mechanism, vertically to travel mechanism; Blood on the said running gear is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin and is connected with automatic application of sample pipeline, and the cleaning needle on the said running gear is connected with automatic detergent line; Said support top is provided with the conveyer of blood clotting cup, the conveyer of said blood clotting cup comprise be positioned at blood coagulation proving installation top grab cup device and the winding transport that is positioned at blood coagulation proving installation one side; The said cup device of grabbing is installed on the running gear, and this running gear comprises horizontal running mechanism, vertical travel mechanism; Said winding transport is provided with blood clotting cup band, and this winding transport below is provided with delivery chute, and the output terminal of said delivery chute extends to and the corresponding position of preheating position in storehouse, transport is housed below the said delivery chute.
Compared with present technology the present invention has following advantage:
1, adopts emission type test cup down in the platelet aggregation proving installation of the present invention, make application of sample, detection, cleaning, removal waste fluid, dry entire exercise form the integral body of a robotization control.
2, the present invention has overcome the defective of prior art, and automatic application of sample, detection automatically, the automatic cleaning are incorporated on the analyser, has realized application of sample, detection, cleaning, removal waste fluid, dry full-automatic, has improved the test speed of clinical examination greatly.
3, full-automatic detection mode of the present invention makes operating personnel avoid having protected the healthy of operator greatly because of directly contacting contaminated risk with patient's blood sample, has simultaneously also avoided the operator pollution of blood sample to be influenced the accuracy of detection.
Below in conjunction with accompanying drawing and embodiment the present invention is described further.
Description of drawings
Fig. 1 is the FB(flow block) of embodiments of the invention one
Fig. 2 is the FB(flow block) of embodiments of the invention two
Fig. 3 is the structural representation (front view) of automatic coagulation analyzer of the present invention
Fig. 4 is the vertical view of automatic coagulation analyzer of the present invention
Fig. 5 is the left view of automatic coagulation analyzer of the present invention
Fig. 6 is that blood is assembled sample needle and needle cleaning device structural representation (the A-A cut-open view of Fig. 5)
Fig. 7 is test cup structure figure of the present invention
Fig. 8 is the structural representation of winding transport of the present invention
Fig. 9 is the piping diagram of automatic coagulation analyzer of the present invention
Embodiment
Embodiment one:
Referring to Fig. 1; The assay method of platelet aggregation property of the present invention and clotting factor; Use a kind of automatic coagulation analyzer; Said automatic coagulation analyzer comprises conveyer, blood gathering sample needle and needle cleaning device, blood coagulation blood sample pin and the clotting reagent needle device of platelet aggregation proving installation, blood coagulation proving installation, sample disc device, reagent bottle device, cleaning disc device, blood clotting cup; Said blood is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin and is connected with automatic application of sample pipeline, and said cleaning needle is connected with automatic detergent line.The technology contents of automatic coagulation analyzer is referring to the description of embodiment three, and platelet aggregation property is measured according to photoelectric turbidimetry, and clotting factor is measured according to the double magnetic circuit paramagnetic particle method, and determination step is:
A, collection blood samples of patients sample (pressing the collection of examination criteria consumption, normally 5ml) carry out anti-freezing to this blood sample and handle, and generate anticoagulated whole blood, with the anticoagulated whole blood centrifugal treating, and preparation platelet rich plasma and platelet poor plasma; In the present embodiment, the blood samples of patients sample carries out anti-freezing with blood anticoagulant 3.8% (concentration) sodium citrate, and the ratio of anti-coagulants and blood is 1: 9; Anticoagulated whole blood is injected sample hose, and to be moved into hydro-extractor then centrifugal, earlier with low-speed centrifugal (500～800 rev/mins, centrifugal 5 minutes); Separate preparation PRP (being platelet rich plasma); With high speed centrifugation (3000 rev/mins, centrifugal 10 minutes), separate preparation PPP (being platelet poor plasma) again; In the present embodiment, also can adopt the index of control centrifugal force to realize separating, the present invention also can adopt LDI4-0.8 horizontal hydro-extractor (Beijing Medical Centrifugal Machine Factory).
B, platelet rich plasma for preparing and platelet poor plasma are placed in the sample disc device, sample number is set; Be provided with corresponding calculated machine operation program in the automatic coagulation analyzer of the present invention in advance, accomplish the setting of sample number by operating personnel's operational computations machine; Said sample number can be an electronic marker.
C, with the reagent bottle device that the platelet aggregation derivant for preparing and clotting reagent are put into automatic coagulation analyzer, cryopreservation is subsequent use; ADP more (adenosine diphosphate (ADP)) is generally used in the preparation of platelet aggregation derivant, and ADP generally is made into 3000umol/l (concentration) with physiological saline, and in packing in a small amount below-20 ℃, low temperature is frozen.Face with preceding and redissolve at room temperature, and be diluted to 300umol/l with physiological saline, put into the reagent bottle device of full-automatic platelet aggregation instrument then, it is subsequent use to give stored refrigerated, refrigerated storage temperature 5-20 ℃; Derivant also can use a kind of among ADR (adrenal gland), COL (collagen), ARA (arachidonic acid), the RIS (ristomycin).Clotting reagent has PT (prothrombin time) reagent, APTT (activated partial thromboplastin time) reagent, TT (thrombin time) reagent, FIB (fibrinogen assay) reagent; Refrigerated storage temperature 2-8 ℃ storage stability 7 days.
D, order blood are assembled sample needle and from said sample disc device, are pressed examination criteria consumption absorption platelet poor plasma; Inject the test cup of platelet aggregation testing arrangement, the platelet aggregation testing arrangement reads platelet poor plasma sample transmission data and storage automatically; Automatically platelet poor plasma is discharged from test cup bottom then, clean blood automatically and assemble sample needle and test cup; In the present embodiment; The platelet poor plasma consumption is 300ul, and said automatic cleaning blood is assembled sample needle and test cup, is based on pre-set program; Use the clear water in the washer bottle to clean blood gathering sample needle and test cup; Blood is assembled sample needle after each absorption sample is accomplished an application of sample action, and all the clear water that can introduce in the washer bottle cleans automatically, cleans the waste liquid that produces and is injected in the waste collection bottle of cleaning disc device by blood gathering sample needle; After test action was accomplished, cleaning needle injected test glass cleaning with the clear water in the washer bottle, and the discarded blood plasma in the test cup is discharged in the waste collection bottle with the vent valves of cleaning waste liquid bottom it; Start heater then to the oven dry of test cup.
E, order blood are assembled sample needle and from said sample disc device, are pressed examination criteria consumption absorption platelet rich plasma; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads platelet rich plasma sample transmission data and storage automatically, cleans blood then automatically and assembles sample needle; In the present embodiment; The platelet rich plasma consumption is 300ul, and said automatic cleaning blood is assembled sample needle, is according to pre-set program; Use the clear water in the washer bottle to clean blood gathering sample needle; Blood is assembled sample needle after each absorption sample is accomplished an application of sample action, and the clear water that all can introduce in the washer bottle cleans automatically, cleans the waste liquid that produces and is injected in the waste collection bottle of cleaning disc device by blood gathering sample needle.
F, order blood are assembled sample needle and from the reagent bottle device, are pressed examination criteria consumption absorption platelet aggregation derivant; Inject the test cup of platelet aggregation testing arrangement; The platelet aggregation testing arrangement reads the platelet rich plasma sample transmission data in the platelet aggregation forming process in the stipulated time automatically; Describe aggregation curve and storage, automatically the platelet rich plasma sample is discharged from test cup bottom then, next clean blood automatically and assemble sample needle and test cup; In the present embodiment; Platelet aggregation derivant consumption is 5ul, and said automatic cleaning blood is assembled sample needle and test cup, is based on pre-set program; The cleaning fluid that blood is assembled in the sample needle cleaning liquid suction bottle cleans automatically; Blood is assembled sample needle after each absorption sample is accomplished an application of sample action, all can cleaning liquid suction clean automatically, cleans the waste liquid that produces and is injected in the waste collection bottle of cleaning disc device by blood gathering sample needle; After test action was accomplished, cleaning needle injected the test cup with the cleaning fluid in the cleaning liquid suction bottle and cleans, and the waste liquid in the test cup is discharged in the waste collection bottle through the vent valves of its bottom; Start heater then to the oven dry of test cup.
The blood clotting cup that the conveyer of G, blood clotting cup will contain steel ball is delivered on the preheating position in storehouse of blood coagulation proving installation, and application software is discerned sample number, confirms the position of same patient's blood sample in the sample disc device.
H, order blood coagulation blood sample pin are pressed the platelet poor plasma that the examination criteria consumption is drawn same patient from said sample disc device, inject the blood clotting cup, automatically the preheating platelet poor plasma; In the present embodiment, carry out prothrombin time (PT) and APTT (APTT), the platelet poor plasma consumption is 100ul; Carry out thrombin time test (TT) and fibrinogen assay (FIB), the platelet poor plasma consumption is 200ul.Below the preheating position in storehouse, be provided with heating arrangement, be no less than 3 minutes preheating time, temperature is controlled at 37 ℃.
The conveyer of I, blood clotting cup is delivered to the blood clotting cup on the detection position in storehouse of blood coagulation proving installation; Drive steel ball continuous vibration at the bottom of the blood clotting cup; From the reagent bottle device, press the examination criteria consumption according to the mensuration project order clotting reagent pin of selecting and draw the reagent of selecting, inject selected blood clotting cup; In blood clotting cup both sides one group of drive coil is set respectively, produces constant alternately electromagnetic field, make the little steel ball of degaussing special in the blood clotting cup keep continuous vibration, steel ball is along the bottom to-and-fro movement of blood clotting cup, and reagent is as long as cover the steel ball of vibration.In the present embodiment, the consumption of PT (prothrombin time) reagent is 200ul, and the consumption of APTT (APTT) reagent, TT (thrombin time test) reagent, FIB (fibrinogen assay) reagent is 100ul.If set different patients' blood sample carried out the test of identical items simultaneously; Then behind the EOT of this project, just clean the clotting reagent pin automatically; If set the blood sample of same patient carried out the test of disparity items simultaneously; After then each completion once adds the reagent action, all can clean the clotting reagent pin automatically.
J, blood coagulation proving installation read the amplitude variations of steel ball automatically, confirm solidifying point, and the clotting factor data of measuring are stored, and clean blood coagulation blood sample pin and clotting reagent pin then automatically, and the conveyer of blood clotting cup abandons used blood clotting cup; In the present embodiment, along with fibrinous generation increases, the viscosity of platelet poor plasma increases, and the amplitude of steel ball weakens gradually.When amplitude fading is confirmed solidifying point to 50% the time.Said automatic cleaning blood coagulation blood sample pin and clotting reagent pin; Be according to pre-set program; Cleaning fluid in blood coagulation blood sample pin and the clotting reagent pin cleaning liquid suction bottle cleans automatically; Blood coagulation blood sample pin and clotting reagent pin all can cleaning liquid suction clean after each absorption sample is accomplished an application of sample action automatically, clean in the waste collection bottle of waste liquid by blood coagulation blood sample pin and clotting reagent pin injection cleaning disc device that produces.
The platelet aggregation rate of K, the tested blood sample of calculating, the mensuration result with platelet aggregation property and clotting factor generates test report according to the patient information of being imported simultaneously; Said blood platelet MA account form is by four groups of transmittance-time-variable datas of platelet aggregation proving installation output, goes out the blood platelet MA according to these four groups of data computation then.Computer program among the present invention can be according to setting; Calculate the platelet aggregation rate that whole piece is assembled any time point on the curve according to a computing formula, 3-4 time point selected in general clinical meeting, then; Assemble on the curve from whole piece again; Find a platelet aggregation rate, like this, clinical experiment report can be provided the platelet aggregation rate and the MA of 3-4 time point.
The present invention calculates platelet aggregation rate according to the following equation:
In the formula:
PRP
BeforePenetrability for the platelet rich plasma of platelet aggregation before forming is directly proportional with turbidity, is inversely proportional to luminous flux.
PRP
AfterPenetrability for the platelet rich plasma of platelet aggregation after forming.
PPP is the penetrability of disperse phase platelet poor plasma.
The platelet aggregation derivant of this employing is like ADP, and automatic coagulation analyzer of the present invention is observed the gathering time that ADP induces, simultaneously; Real-time depict a time dependent gathering curve of PAR; Then, can be according to setting, the platelet aggregation rate when providing certain several time:
60 seconds platelet aggregation rates that the ADP derivant is induced;
120 seconds platelet aggregation rates that the ADP derivant is induced;
180 seconds platelet aggregation rates that the ADP derivant is induced;
240 seconds platelet aggregation rates that the ADP derivant is induced;
300 seconds platelet aggregation rates that the ADP derivant is induced;
The maximum platelet aggregation rate that the ADP derivant is induced;
The blood platelet MA is exactly the data of maximum that of platelet aggregation rate in the whole gathering curve.
In the present embodiment; Four test cup positions (constituting four TCH test channels) of automatic coagulation analyzer can be measured the curve of platelet aggregation of certain a blood sample separately according to above-mentioned setting; And can set according to the user and provide in the curve arbitrarily a bit or the platelet aggregation rate of multiple spot; Then, provide the blood platelet MA again.
In the present embodiment, described four TCH test channels can be measured 4 parts of blood samples (4 patients' blood samples) simultaneously.
In the present embodiment, the data of being measured are handled all belong to the proven technique content, be not described in detail at this.
In the present embodiment, the action of the data test of platelet aggregation property and clotting factor is all undertaken by computer program control sequence.In the present embodiment, said testing sequence can be adjusted as required at any time.
Embodiment two:
Referring to Fig. 2; In the present embodiment; The assay method of platelet aggregation property and clotting factor; Use a kind of automatic coagulation analyzer; Said automatic coagulation analyzer comprises conveyer, blood gathering sample needle and needle cleaning device, blood coagulation blood sample pin and the clotting reagent needle device of platelet aggregation proving installation, blood coagulation proving installation, sample disc device, reagent bottle device, cleaning disc device, blood clotting cup, and said blood is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin and is connected with automatic application of sample pipeline, and said cleaning needle is connected with automatic detergent line.The technology contents of automatic coagulation analyzer is referring to the description of embodiment three, and clotting factor is measured according to the double magnetic circuit paramagnetic particle method, and platelet aggregation property is measured according to photoelectric turbidimetry.
Present embodiment is set determination step through application software according to the needs of practical measurement as follows, and concrete assay method is identical with the assay method of use among the embodiment one, and with reference to disclosed content among the embodiment one, concrete determination step is:
A, collection blood samples of patients sample carry out anti-freezing to this blood sample and handle, and generate anticoagulated whole blood, with the anticoagulated whole blood centrifugal treating, and preparation platelet rich plasma and platelet poor plasma;
B, platelet rich plasma for preparing and platelet poor plasma are placed in the sample disc device, sample number is set;
C, with the reagent bottle device that the platelet aggregation derivant for preparing and clotting reagent are put into automatic coagulation analyzer, cryopreservation is subsequent use;
The blood clotting cup that the conveyer of D, blood clotting cup will contain steel ball is delivered on the preheating position in storehouse of blood coagulation proving installation; Application software is discerned sample number; Order blood coagulation blood sample pin is pressed the examination criteria consumption and is drawn platelet poor plasma from said sample disc device; Inject the blood clotting cup, automatically the preheating platelet poor plasma;
The conveyer of E, blood clotting cup is delivered to the blood clotting cup on the detection position in storehouse of blood coagulation proving installation; Drive steel ball continuous vibration at the bottom of the blood clotting cup; From the reagent bottle device, press the examination criteria consumption according to the mensuration project order clotting reagent pin of selecting and draw the reagent of selecting, inject selected blood clotting cup;
F, blood coagulation proving installation read the amplitude variations of steel ball automatically, confirm solidifying point, and the clotting factor data of measuring are stored, and the conveyer of blood clotting cup abandons used blood clotting cup;
G, application software are discerned sample number; Order blood is assembled sample needle and from said sample disc device, is pressed the platelet poor plasma that the examination criteria consumption is drawn same patient; Inject the test cup of platelet aggregation proving installation, the platelet aggregation proving installation reads platelet poor plasma sample transmission data and storage automatically; Automatically platelet poor plasma is discharged from test cup bottom then, clean the test cup automatically;
H, order blood are assembled sample needle and from said sample disc device, are pressed the platelet rich plasma that the examination criteria consumption is drawn same patient; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads platelet rich plasma sample transmission data and storage automatically, cleans blood then automatically and assembles sample needle;
I, order blood are assembled sample needle and from the reagent bottle device, are pressed examination criteria consumption absorption platelet aggregation derivant; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads the platelet rich plasma sample transmission data in the platelet aggregation forming process in the stipulated time automatically; Describe to assemble curve and storage, automatically the platelet rich plasma sample is discharged from test cup bottom then, clean blood then automatically and assemble sample needle and test cup;
J, calculate the platelet aggregation rate of tested blood sample, with the mensuration result of platelet aggregation property and clotting factor according to the patient information generation test report of being imported.
Embodiment three:
Referring to Fig. 3, Fig. 4, Fig. 5, Fig. 7, Fig. 9; (Fig. 3 is the situation of taking off the analyser front panel), (Fig. 4 is the layout situation of analyser testing stand), (Fig. 5 is the situation of taking off the analyser side panel); Automatic coagulation analyzer of the present invention; A support is arranged; Support is provided with photoelectric turbidimetry test circuit, double magnetic circuit paramagnetic particle method test circuit, automatic control circuit and matcher, and platelet aggregation proving installation 1, sample disc device 2, cleaning disc device 3, reagent bottle device 4, blood coagulation proving installation 5 are set on the testing stand 13 of said support; Be provided with a plurality of test cups 11 in the said platelet aggregation proving installation, the detection light path system be set, comprise the lighting transistor circuit and the photoelectric switching circuit of complete configuration in this detection light path system around this test cup; Below the said test cup magnetic stirring apparatus is set.A plurality of sample cups (being used to place blood plasma to be tested) are set on the said sample disc device; Comprise cleaning fluid bottle 31 and waste collection bottle 32 in the said cleaning disc device; On the said reagent bottle device low temperature holding device is set; Said blood coagulation proving installation middle part is provided with a plurality of tactic preheating positions in storehouse 51 and detects position in storehouse 52, and said detection position in storehouse symmetria bilateralis is provided with two groups of drive coils, is provided with one group under the said detection position in storehouse and measures coil; Said testing stand top is provided with blood and assembles sample needle device, needle cleaning device 6, blood coagulation blood sample needle device, clotting reagent needle device 7; Said blood is assembled sample needle device, needle cleaning device, blood coagulation blood sample needle device, clotting reagent needle device and is installed on the running gear 10, this running gear comprise vertical travel mechanism, laterally travel mechanism, vertically to travel mechanism; Be provided with blood in the blood gathering sample needle device on the said running gear and assemble sample needle, be provided with cleaning needle in the needle cleaning device, be provided with blood coagulation blood sample pin in the blood coagulation blood sample needle device, be provided with the clotting reagent pin in the clotting reagent needle device; Said blood is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin and is connected with automatic application of sample pipeline, and the cleaning needle on the said running gear is connected with automatic detergent line; Said support top is provided with the conveyer of blood clotting cup, the conveyer of said blood clotting cup comprise be positioned at blood coagulation proving installation top grab cup device 81 and the winding transport 82 that is positioned at blood coagulation proving installation one side; The said cup device of grabbing is installed on the running gear, and this running gear comprises horizontal running mechanism, vertical travel mechanism; Said winding transport is provided with blood clotting cup band; This winding transport below is provided with delivery chute 83; The output terminal of said delivery chute extends to and the corresponding position of preheating position in storehouse, transport is housed below the said delivery chute, is used for blood clotting cup 84 is transported to grabbing the cup device below.
In the present embodiment, the photoelectric turbidimetry test circuit adopts prior art, is not described in detail.Double magnetic circuit paramagnetic particle method test circuit adopts prior art, is not described in detail.Computer Control Technology is adopted in control of the present invention, and its automatic control circuit belongs to the routine techniques means, is not described in detail.
Referring to Fig. 4, Fig. 7, in the present embodiment, be provided with four test cup positions 12 in the platelet aggregation proving installation 1; Test cup 11 is plugged on the test cup position, can take out easily and insert, and magnetic stirring apparatus is set below the test cup; In the test cup, add a little bar magnet; Magnetic agitation motor-driven disk rotates, and little bar magnet rotates thereupon, realizes stirring the effect of plasma sample in the test cup; Belong to prior art, be not described in detail.Platelet aggregation property of the present invention is measured according to photoelectric turbidimetry; The lighting transistor circuit and the photoelectric switching circuit that comprise complete configuration in the used detection light path system; Comprise an infraluminescence diode in the lighting transistor circuit; Comprise an infrared receiving crystal pipe in the photoelectric switching circuit, cup one side of test cup is provided with infrarede emitting diode, and a corresponding side is provided with infrared receiving crystal pipe (owing to being not show among the prior art figure).In order in time to dry to cleaning later test cup; Below the test cup, a heating arrangement is set; This heating arrangement is that wiring board is used in a heating; Through the heating of the lead on this wiring board, be used to reach the effect that makes test zone keep 37 ℃ of constant temperature, and the test cup after cleaning is in time dried.
Referring to Fig. 7, the test cup among the present invention is different with the employed square test cup of platelet aggregation instrument of the prior art, and the cup end of test cup of the prior art is sealed, and the present invention is open.In the present embodiment, test cup 11 comprises the transparent cup 111 of a pipe shape, and bottom of cup is provided with bottom outlet 112, liquid back pipe is installed on the bottom outlet is connect mouth 113, and this liquid back pipe connects mouth and is connected with the liquid back pipe road.The present invention adopts pipe shape cup to be convenient to automatic cleaning, has eliminated the dead angle in the cup, improves cleaning degree; Adopt bottom outlet and liquid back pipe to connect mouth structure; Can through control system realize the test cup externally by with the conversion of emissions status; Make automatic blood coagulation analyzer have the function of automatic washing; Can after having accomplished blood sample test, raffinate be discharged in the waste collection bottle automatically, need not manual work and will test glass to extract and clean.
Referring to Fig. 4; In the present embodiment, position, a plurality of sample cups hole 21 is set on the sample disc device 2, position, sample cup hole is by the lineament layout; Plug-in mounting has the sample cup of PRP, PPP in the sample cup hole, and blood gathering sample needle device can be drawn to detect from sample cup and use plasma sample.After detecting end, sample cup is discarded.
In the present embodiment, cleaning disc device 3 is provided with two independently service sinks, and a service sink is used for the cleaning that blood is assembled sample needle and cleaning needle, and another service sink is used for the cleaning of blood coagulation blood sample pin and clotting reagent pin.Two pin hole shapes of service sink set inside rinse bath 33 and waste collection mouth 34, the bottom is provided with the waste collection bottle, and the waste collection mouth is communicated with the waste collection bottle.Cleaning waste liquid and discarded blood plasma that blood is assembled sample needle device, needle cleaning device, blood coagulation blood sample needle device, clotting reagent needle device and the generation of test cup can be discharged in the waste collection bottle unified the collection.
In the present embodiment, reagent bottle device 4 tops are provided with reagent bottle hole 41, and plug-in mounting reagent bottle in it is preserved platelet aggregation derivant and multiple clotting reagent in reagent bottle.In order to satisfy the temperature requirement of platelet aggregation derivant and clotting reagent, reagent bottle device bottom is provided with semiconductor cooling device.This refrigerating plant is made up of refrigeration circuit, heat radiator and fan, and refrigeration circuit belongs to prior art, is not described in detail.
In the present embodiment; Five to 12 preheating positions in storehouse of the test platform of blood coagulation proving installation 5 middle part series arrangement 51, four detect position in storehouse 52; Detect the position in storehouse symmetria bilateralis two groups of drive coils are set, there is electromagnet drive coil inside, can produce magnetic field after the electromagnet energising.Under detecting position in storehouse one group of measurement coil is set.
Referring to Fig. 3, Fig. 4, Fig. 5, Fig. 6; In the present embodiment; Blood is assembled the sample needle device and is installed on the running gear 10 with needle cleaning device 6, blood coagulation blood sample needle device and clotting reagent needle device 7, and this running gear comprises vertical travel mechanism 1001, horizontal travel mechanism 1002, vertically to travel mechanism 1003; Vertically (X-direction) travel mechanism comprises longitudinal rail, stepper motor, synchronous cog belt, the vertical carriage 1011 of walking, carriage and the synchronous cog belt fixed installation of vertically walking; Laterally (Y direction) travel mechanism comprises cross slide way, stepper motor, synchronous cog belt, the horizontal carriage 1012 of walking, carriage and the synchronous cog belt fixed installation of laterally walking; Vertically comprise four driving key axles 1031, four stepper motors, four synchronous cog belts, eight synchronous pulleys to (Z-direction) travel mechanism; Wherein four synchronous pulleys are set in four driving key axles outsides, and blood is assembled sample needle 61, cleaning needle 62, blood coagulation blood sample pin and clotting reagent pin and fixedly mounted with four synchronous cog belts respectively; Laterally travel mechanism is installed on vertical walking carriage; Vertically be installed on the horizontal walking carriage to travel mechanism; Blood is assembled sample needle, cleaning needle, blood coagulation blood sample pin and clotting reagent pin and is installed in vertically on the walking carriage.Blood is assembled the sample needle device and is comprised blood gathering sample needle, flexible pipe, solenoid valve, suction pumps, measuring element; Needle cleaning device comprises cleaning needle, flexible pipe, solenoid valve, suction pumps, measuring element; Blood coagulation blood sample needle device comprises blood coagulation blood sample pin, flexible pipe, solenoid valve, suction pumps, measuring element; The clotting reagent needle device comprises clotting reagent pin, flexible pipe, solenoid valve, suction pumps, measuring element; This device belongs to the prior art scope, is not described in detail.
Referring to Fig. 9, the pipeline 9 of present embodiment comprises automatic application of sample pipeline and detergent line automatically.Blood is assembled sample needle 61, blood coagulation blood sample pin 71 and clotting reagent pin 72 and is connected with automatic application of sample pipeline, and this pipeline comprises solenoid valve, glass tube syringe pump 91, is used for drawing quantitative blood samples from sample cup, injects test cup or blood clotting cup.The glass tube syringe pump is provided with three, is connected through pipeline with the clotting reagent pin with blood gathering sample needle, blood coagulation blood sample pin respectively.Present embodiment can also adopt the structure of 3 pins, and promptly platelet aggregation property testing and clotting factor are tested a shared sample needle, a shared reagent needles of while, and other one is cleaning needle.The automatic detergent line that blood is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin comprises washer bottle 31, five-way valve 92, glass tube syringe pump; Deposit has clear water in the washer bottle; (washer bottle is the special-purpose cleaning fluid of deposit usually, so that effectively clean protein matter, also can put clear water to be used for cleaning blood gathering sample needle, blood coagulation blood sample pin and clotting reagent pin; But can lower the effect of cleaning).Cleaning needle 62 devices are connected with automatic detergent line, and the automatic detergent line of cleaning needle comprises washer bottle, scavenging pump 95; Be used for cleaning the test cup.The liquid back pipe of test cup connects mouth and is connected with the liquid back pipe road, and the liquid back pipe road comprises waste collection bottle 32, negative pressure pump 94, six-way valve 93, liquid level detection device, is used for the raffinate of testing cup, discarded blood plasma, cleans waste collection.
Referring to Fig. 3, Fig. 4, Fig. 8, in the present embodiment, the conveyer of blood clotting cup comprise be positioned at blood coagulation proving installation top grab cup device 81 and the winding transport 82 that is positioned at blood coagulation proving installation one side; The said cup of grabbing is equipped with a pedestal, and pedestal top is provided with an electromagnetic driver, and said pedestal bottom is provided with a pair of body that grasps, and said two grasp on the body to be provided with and grasp spring; Said concrete structure of grabbing cup device can be with reference to disclosed content in the Chinese patent 200920107535.3.The said cup device of grabbing is installed on the running gear, and this running gear comprises horizontal running mechanism, vertical travel mechanism; Said horizontal running mechanism comprises horizontally disposed slide rail, stepper motor, synchronous cog belt, walking in a horizontal state carriage; Said vertical travel mechanism comprises the slide rail, stepper motor, synchronous cog belt of vertical setting, the vertical carriage of walking; Said winding transport is provided with the blood clotting cup and is with 821, and described blood clotting cup band is by pre-installing one by one on a long transparent base band.The shape of blood clotting cup can be referring to Chinese patent: 200930126003.X, and the blood clotting cup is up big and down small prismatoid, base seal, and rectangular opening is opened on top, places a measuring steel ball in each blood clotting cup.This winding transport below is provided with delivery chute, has in this delivery chute to press from both sides along the cup that pushes away that groove moves, and pushes away the cup folder and is provided with a U-shaped mouth, and the width of U-shaped mouth is slightly larger than the bottom width of blood clotting cup.The output terminal of said delivery chute extends to and the corresponding position of preheating position in storehouse, belt conveyor is housed below the said delivery chute.Whole transportation process is sent unified instruction directs by programming controller and is accomplished, and all provide the motor of power to be stepper motor.
Claims (7)
1. the assay method of platelet aggregation property and clotting factor; It is characterized in that: use a kind of automatic coagulation analyzer; Said automatic coagulation analyzer comprises conveyer, blood gathering sample needle device, needle cleaning device, blood coagulation blood sample needle device and the clotting reagent needle device of platelet aggregation proving installation, blood coagulation proving installation, sample disc device, reagent bottle device, cleaning disc device, blood clotting cup; Be provided with blood in the described blood gathering sample needle device and assemble sample needle; Be provided with cleaning needle in the described needle cleaning device, be provided with blood coagulation blood sample pin in the described blood coagulation blood sample needle device, be provided with the clotting reagent pin in the described clotting reagent needle device; Said blood is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin and is connected with automatic application of sample pipeline, and said cleaning needle is connected with automatic detergent line;
Platelet aggregation property is measured according to photoelectric turbidimetry, and clotting factor is measured according to the double magnetic circuit paramagnetic particle method, and determination step is:
A, blood sample is carried out anti-freezing handle, generate anticoagulated whole blood, with the anticoagulated whole blood centrifugal treating, preparation platelet rich plasma and platelet poor plasma;
B, platelet rich plasma for preparing and platelet poor plasma are placed in the sample disc device, sample number is set;
C, with the reagent bottle device that the platelet aggregation derivant for preparing and clotting reagent are put into automatic coagulation analyzer, cryopreservation is subsequent use;
D, order blood are assembled sample needle and from said sample disc device, are pressed examination criteria consumption absorption platelet poor plasma; Inject the test cup of platelet aggregation proving installation, the platelet aggregation proving installation reads platelet poor plasma sample transmission data and storage automatically; Automatically platelet poor plasma is discharged from test cup bottom then, clean the test cup automatically;
E, order blood are assembled sample needle and from said sample disc device, are pressed examination criteria consumption absorption platelet rich plasma; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads platelet rich plasma sample transmission data and storage automatically, cleans blood then automatically and assembles sample needle;
F, order blood are assembled sample needle and from the reagent bottle device, are pressed examination criteria consumption absorption platelet aggregation derivant; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads the platelet rich plasma sample transmission data in the platelet aggregation forming process in the stipulated time automatically; Describe to assemble curve and storage, automatically the platelet rich plasma sample is discharged from test cup bottom then, clean blood then automatically and assemble sample needle and test cup;
The blood clotting cup that the conveyer of G, blood clotting cup will contain steel ball is delivered on the preheating position in storehouse of blood coagulation proving installation, and application software is discerned sample number;
H, order blood coagulation blood sample pin are pressed the platelet poor plasma that the examination criteria consumption is drawn same patient from said sample disc device, inject the blood clotting cup, automatically the preheating platelet poor plasma;
The conveyer of I, blood clotting cup is delivered to the blood clotting cup on the detection position in storehouse of blood coagulation proving installation; Drive steel ball continuous vibration at the bottom of the blood clotting cup; From the reagent bottle device, press the examination criteria consumption according to the mensuration project order clotting reagent pin of selecting and draw the reagent of selecting, inject selected blood clotting cup;
J, blood coagulation proving installation read the amplitude variations of steel ball automatically, confirm solidifying point, and the clotting factor data of measuring are stored, and the conveyer of blood clotting cup abandons used blood clotting cup;
K, calculate the platelet aggregation rate of tested blood sample, with the mensuration result of platelet aggregation property and clotting factor according to the patient information generation test report of being imported.
2. the assay method of platelet aggregation property and clotting factor; It is characterized in that: use a kind of automatic coagulation analyzer; Said automatic coagulation analyzer comprises conveyer, blood gathering sample needle device, needle cleaning device, blood coagulation blood sample needle device and the clotting reagent needle device of platelet aggregation proving installation, blood coagulation proving installation, sample disc device, reagent bottle device, cleaning disc device, blood clotting cup; Be provided with blood in the described blood gathering sample needle device and assemble sample needle; Be provided with cleaning needle in the described needle cleaning device, be provided with blood coagulation blood sample pin in the described blood coagulation blood sample needle device, be provided with the clotting reagent pin in the described clotting reagent needle device; Said blood is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin and is connected with automatic application of sample pipeline, and said cleaning needle is connected with automatic detergent line;
Clotting factor is measured according to the double magnetic circuit paramagnetic particle method, and platelet aggregation property is measured according to photoelectric turbidimetry, and determination step is:
A, blood sample is carried out anti-freezing handle, generate anticoagulated whole blood, with the anticoagulated whole blood centrifugal treating, preparation platelet rich plasma and platelet poor plasma;
B, platelet rich plasma for preparing and platelet poor plasma are placed in the sample disc device, sample number is set;
C, with the reagent bottle device that the platelet aggregation derivant for preparing and clotting reagent are put into automatic coagulation analyzer, cryopreservation is subsequent use;
The blood clotting cup that the conveyer of D, blood clotting cup will contain steel ball is delivered on the preheating position in storehouse of blood coagulation proving installation; Order blood coagulation blood sample pin is pressed the examination criteria consumption and is drawn platelet poor plasma from said sample disc device; Inject the blood clotting cup, automatically the preheating platelet poor plasma;
The conveyer of E, blood clotting cup is delivered to the blood clotting cup on the detection position in storehouse of blood coagulation proving installation; Drive steel ball continuous vibration at the bottom of the blood clotting cup; From the reagent bottle device, press the examination criteria consumption according to the mensuration project order clotting reagent pin of selecting and draw the reagent of selecting, inject selected blood clotting cup;
F, blood coagulation proving installation read the amplitude variations of steel ball automatically, confirm solidifying point, and the clotting factor data of measuring are stored, and the conveyer of blood clotting cup abandons used blood clotting cup;
G, application software are discerned sample number; Order blood is assembled sample needle and from said sample disc device, is pressed the platelet poor plasma that the examination criteria consumption is drawn same patient; Inject the test cup of platelet aggregation proving installation, the platelet aggregation proving installation reads platelet poor plasma sample transmission data and storage automatically; Automatically platelet poor plasma is discharged from test cup bottom then, clean the test cup automatically;
H, order blood are assembled sample needle and from said sample disc device, are pressed the platelet rich plasma that the examination criteria consumption is drawn same patient; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads platelet rich plasma sample transmission data and storage automatically, cleans blood then automatically and assembles sample needle;
I, order blood are assembled sample needle and from the reagent bottle device, are pressed examination criteria consumption absorption platelet aggregation derivant; Inject the test cup of platelet aggregation proving installation; The platelet aggregation proving installation reads the platelet rich plasma sample transmission data in the platelet aggregation forming process in the stipulated time automatically; Describe to assemble curve and storage, automatically the platelet rich plasma sample is discharged from test cup bottom then, clean blood then automatically and assemble sample needle and test cup;
J, calculate the platelet aggregation rate of tested blood sample, with the mensuration result of platelet aggregation property and clotting factor according to the patient information generation test report of being imported.
3. automatic coagulation analyzer; A support is arranged; Support is provided with photoelectric turbidimetry test circuit, double magnetic circuit paramagnetic particle method test circuit, automatic control circuit and matcher, it is characterized in that: platelet aggregation proving installation, sample disc device, cleaning disc device, reagent bottle device, blood coagulation proving installation are set on the testing stand of said support; Be provided with a plurality of test cups in the said platelet aggregation proving installation, the detection light path system be set, comprise the lighting transistor circuit and the photoelectric switching circuit of complete configuration in this detection light path system around this test cup; Below the said test cup magnetic stirring apparatus is set; On the said sample disc device a plurality of sample cups are set; Comprise cleaning fluid bottle and waste collection bottle in the said cleaning disc device; On the said reagent bottle device low temperature holding device is set; Said blood coagulation proving installation middle part is provided with a plurality of tactic preheating positions in storehouse and detects position in storehouse, and said detection position in storehouse symmetria bilateralis is provided with two groups of drive coils, under the said detection position in storehouse measurement coil is set; Said testing stand top is provided with blood and assembles sample needle device, needle cleaning device, blood coagulation blood sample needle device, clotting reagent needle device; Said blood is assembled sample needle device, needle cleaning device, blood coagulation blood sample needle device, clotting reagent needle device and is installed on the running gear, this running gear comprise vertical travel mechanism, laterally travel mechanism, vertically to travel mechanism; Be provided with blood in the said blood gathering sample needle device and assemble sample needle, be provided with cleaning needle in the needle cleaning device, be provided with blood coagulation blood sample pin in the blood coagulation blood sample needle device, be provided with the clotting reagent pin in the clotting reagent needle device; Blood on the said running gear is assembled sample needle, blood coagulation blood sample pin and clotting reagent pin and is connected with automatic application of sample pipeline, and the cleaning needle on the said running gear is connected with automatic detergent line; Said support top is provided with the conveyer of blood clotting cup, the conveyer of said blood clotting cup comprise be positioned at blood coagulation proving installation top grab cup device and the winding transport that is positioned at blood coagulation proving installation one side; The said cup device of grabbing is installed on another running gear, and this another running gear comprises horizontal running mechanism, vertical travel mechanism; Said winding transport is provided with blood clotting cup band, and this winding transport below is provided with delivery chute, and the output terminal of said delivery chute extends to and the corresponding position of preheating position in storehouse, transport is housed below the said delivery chute.
4. automatic coagulation analyzer according to claim 3 is characterized in that: described test cup comprises the transparent body of a pipe shape, and bottom of cup is provided with bottom outlet, liquid back pipe is installed on the bottom outlet is connect mouth, and this liquid back pipe connects mouth and is connected with the liquid back pipe road.
5. automatic coagulation analyzer according to claim 3 is characterized in that: below the described test cup heating arrangement is set.
6. automatic coagulation analyzer according to claim 3 is characterized in that: described reagent bottle device bottom is provided with semiconductor cooling device.
7. automatic coagulation analyzer according to claim 3 is characterized in that: below said preheating position in storehouse and the detection position in storehouse heating arrangement is set.
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| CN104731153A (en) * | 2015-01-27 | 2015-06-24 | 北京利德曼生化股份有限公司 | Closed-loop regulation drive circuit for high-power semiconductor chilling plate |
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Effective date of registration: 20121231 Address after: 100085 Beijing city Haidian District Qunying Science Park Building 5, two floor East Patentee after: Pulisheng Instruments Co., Ltd., Beijing Patentee after: Chen Sheng Address before: 100085 Beijing city Haidian District Qunying Science Park Building 5, two floor East Patentee before: Pulisheng Instruments Co., Ltd., Beijing |