CN110631964B - Magnetic bead method detection method and magnetic bead method detection device - Google Patents
Magnetic bead method detection method and magnetic bead method detection device Download PDFInfo
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- 238000012360 testing method Methods 0.000 claims abstract description 65
- 108010049003 Fibrinogen Proteins 0.000 claims abstract description 17
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- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 108010094028 Prothrombin Proteins 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
- G01N11/10—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by moving a body within the material
- G01N11/16—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by moving a body within the material by measuring damping effect upon oscillatory body
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Abstract
The embodiment of the application provides a magnetic bead method detection method and a magnetic bead detection device, which are used for improving the performance of magnetic bead method test. The method comprises the following steps: obtaining a test item of a reaction sample; determining a sample ratio according to the test item, wherein the sample ratio is used for representing the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; determining a driving parameter according to the sample duty ratio; and driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
Description
Technical Field
The application relates to the field of medical equipment, in particular to a magnetic bead method detection method and a magnetic bead detection device.
Background
A coagulation analyzer is an instrument that is used clinically to perform coagulation and anticoagulation, fibrinolysis and antifibrinolytic functional analysis of a patient's blood. Among other things, coagulation methods can be used to determine the coagulation properties or fibrinolytic properties of a blood sample. Magnetic bead methods are currently used to test coagulation characteristics of blood samples. The magnetic bead method comprises a double-magnetic-circuit magnetic bead method, a photo-electromagnetic bead method and the like, and the reaction end point of the coagulation process is judged according to the viscosity characteristic change in the reaction system. The principle is as follows: a group of driving coils are arranged on two sides of the test cup and used for generating a constant alternating electromagnetic field so that the special demagnetizing small steel balls in the test cup keep equal-amplitude oscillating motion; and the other group of measuring coils or photoelectric sensors of the instrument can record the movement change of the small steel balls, and when the movement amplitude is attenuated to 50%, the solidification end point is judged.
The measurement process of the magnetic bead method belongs to a dynamic measurement process, so that the magnetic bead method is more easily influenced by dynamic interference factors, and the magnetic bead can swing on one side and cannot be tested when serious.
Disclosure of Invention
The embodiment of the application provides a magnetic bead method detection method and a magnetic bead detection device, which are used for improving the performance of magnetic bead method test.
In a first aspect, an embodiment of the present application provides a magnetic bead detection method, specifically including: obtaining a test item of a reaction sample; determining a sample ratio according to the test item, wherein the sample ratio is used for representing the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; determining a driving parameter according to the sample duty ratio; and driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
In a second aspect, an embodiment of the present application provides a magnetic bead detection method, specifically including: acquiring a gravity component and a buoyancy component of the magnetic beads in the horizontal direction; determining a driving parameter adjustment amount according to the gravity component and the buoyancy component; determining a driving parameter according to the driving parameter adjustment amount; and driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
In a third aspect, an embodiment of the present application provides a magnetic bead method detection apparatus, specifically including: input device, processor, output device; the input device is used for acquiring test items of a reaction sample; the processor is used for determining a sample ratio according to the test item, wherein the sample ratio is used for representing the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; determining a driving parameter according to the sample duty ratio; driving the magnetic beads according to the driving parameters, and detecting a reaction end point of the reaction sample; the output device is used for outputting the reaction end point.
In a fourth aspect, an embodiment of the present application provides a magnetic bead method detection apparatus, specifically including: an input device, a processor, and an output device; the input device is used for acquiring a gravity component and a buoyancy component of the magnetic beads; the processor is used for determining a driving parameter adjustment amount according to the gravity component and the buoyancy component; determining a driving parameter according to the driving parameter adjustment amount; driving the magnetic beads according to the driving parameters, and detecting a reaction end point of the reaction sample; the output device is used for outputting the reaction end point.
From the above technical solutions, the embodiments of the present application have the following advantages: when the magnetic bead method detects a reaction sample, the driving voltage of the magnetic bead method detection device is determined according to the sample ratio of fibrinogen in the reaction sample, so that different reaction samples are guaranteed to have corresponding driving voltages, the abnormal frequency of a measurement waveform is effectively reduced, the measurement range of the magnetic bead method detection device is widened, and the testing performance of the magnetic bead method test is improved.
Drawings
FIG. 1 is a schematic block diagram of an exemplary bead detection apparatus 100 according to an embodiment of the present application;
FIG. 2 is a schematic diagram of the principle of testing the magnetic bead method in a dual magnetic circuit magnetic bead method sensor measurement model;
FIG. 3 is a schematic diagram of a force analysis of a magnetic bead in a test cup;
FIG. 4 is a schematic diagram showing an embodiment of the detection method by the magnetic bead method in the embodiment of the present application;
FIG. 5 is a schematic diagram of another embodiment of the detection method according to the magnetic bead method in the embodiment of the present application;
FIG. 6 is a schematic diagram of an embodiment of a magnetic bead detection apparatus according to an embodiment of the present application;
FIG. 7 is a schematic diagram of another embodiment of a magnetic bead detection device according to an embodiment of the present application;
FIG. 8 is a schematic diagram of another embodiment of a magnetic bead detection device according to an embodiment of the present application;
Fig. 9 is a schematic diagram of another embodiment of a magnetic bead detection device according to an embodiment of the present application.
Detailed Description
The embodiment of the application provides a magnetic bead method detection method and a magnetic bead method detection device, which are used for improving the performance of magnetic bead method test.
The terms "first," "second," "third," "fourth" and the like in the description and in the claims of this application and in the above-described figures, if any, are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that the embodiments described herein may be implemented in other sequences than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
Fig. 1 is a schematic block diagram illustrating an exemplary magnetic bead detection apparatus 100 according to an embodiment of the present application. The bead method detection apparatus 100 includes an input device 101, a processor 102, and an output device 103. The input device 101 may be used to obtain test items of a reaction sample, i.e. a user may directly input test items of the reaction sample through the input device 101; the processor 102 determines a sample ratio of fibrinogen in the reaction sample from the test item, wherein the sample ratio is indicative of a percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; the processor 102 then determines a driving parameter based on the sample duty cycle; driving the magnetic beads according to the driving parameters, and detecting a reaction end point of the reaction sample; finally, the reaction end point is outputted through the output device 103.
In this embodiment of the present application, the output device 103 of the magnetic bead detection apparatus 100 may be a touch display screen, a liquid crystal display screen, or the like, or may be an independent display device such as a liquid crystal display, a television, or the like, which is independent of the magnetic bead detection apparatus 100, or may be a display screen on an electronic device such as a mobile phone, a tablet computer, or the like.
In an exemplary dual magnetic circuit magnetic bead method sensor measurement model as shown in fig. 2, the test principle of the magnetic bead method in the embodiment of the present application is as follows: a group of driving coils are arranged on two sides of the test cup and used for generating a constant alternating electromagnetic field so that the special demagnetizing small steel balls in the test cup keep equal-amplitude oscillating motion. After the coagulation activator is added, the viscosity of a reaction system is continuously increased along with the conversion of fibrinogen into crosslinked fibrin, the movement amplitude of the small steel balls is gradually weakened, the movement change of the small steel balls can be recorded by the other group of measuring coils of the instrument, and when the movement amplitude is attenuated to fifty percent, the reaction end point is judged. And the force analysis of the magnetic beads in the test cup is shown in fig. 3. In an exemplary scenario, the force profile may be as shown in table 1. It is understood that the information in table 1 for the mass, density, sphere radius, volume, density of the reaction system, etc. of the magnetic beads is only one exemplary scheme.
TABLE 1
According to the stress analysis, when the magnetic beads are arranged on two sides of the reaction cup, and when the installation levelness of the magnetic bead sensor is not satisfied with the working condition of the magnetic bead detection device, the component of the magnetic bead gravity in the direction of the magnetic bead electromagnetic force easily causes the waveform of the detection result to be asymmetric and even swing on one side, thereby affecting the detection performance of the magnetic beads. Meanwhile, in the detection process of the magnetic bead method, parameters of a driving coil are required to be matched with the viscosity of a reaction sample. If the initial viscosity of the reaction sample is larger, but the driving force of the driving coil is smaller, the amplitude of the magnetic beads is quickly attenuated to about 0 value after the mixing stage is finished, the viscosity characteristic change of the reaction system cannot be monitored at all, and the information such as the reaction end point and the detection process fail to be acquired; if the initial viscosity of the reaction sample is smaller, but the driving force of the driving coil is larger, the magnetic beads frequently collide with the reaction cup in the detection process, so that the amplitude obtained by the measuring coil is unstable, and the problem that the magnetic beads are self-locked at a certain side of the test cup and cannot move is possibly caused. While the percentage of fibrinogen in the reaction sample (i.e., the sample ratio) in the total volume of the reaction sample can represent the initial viscosity of the reaction sample, in one exemplary embodiment, the relationship between the sample ratio and the initial viscosity of the reaction sample can be as shown in table 2.
Therefore, a user can determine the relation between the sample duty ratio and the driving voltage according to the relation between the sample duty ratio and the initial viscosity, and then adjust the driving parameters of the magnetic bead method detection device.
To solve this problem, the embodiment of the present application may optimize the magnetic bead method detection method in terms of the sample duty ratio and the mounting levelness of the magnetic bead method detection device.
For convenience of understanding, in the embodiments of the present application, related technical terms are explained as follows:
driving voltage: and a voltage value loaded on the driving coil, wherein the driving force generated by the driving coil and used for driving the magnetic beads to move is in a quadratic positive correlation with the driving voltage. In the embodiment of the application, the driving coils positioned on two sides of the test cup in the magnetic bead method detection device can be respectively and independently loaded with driving voltage, and the driving voltage can be independently adjusted.
Drive cycle: the magnetic bead method detection device drives the magnetic beads to swing normally in the test cup.
Duty cycle: the electromagnet on time is proportional to the whole driving period.
Sample ratio: fibrinogen in the reaction sample is a percentage of the total volume of the reaction sample.
The following describes the magnetic bead method detection method in detail with an application scenario of adjusting driving parameters according to the sample ratio of the reaction sample, please refer to fig. 4, and the method is applied to the magnetic bead method detection apparatus 100. The embodiment of the magnetic bead method detection method comprises the following steps:
401. test items of the reaction samples are obtained.
When a user detects a reaction sample, the user firstly obtains test items of the reaction sample. The test items in this embodiment include, but are not limited to, blood coagulation function analysis, blood anticoagulation function analysis.
402. A sample ratio is determined from the test item, the sample ratio being indicative of the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample.
In this embodiment, the manner in which the magnetic bead detection device obtains the sample duty ratio according to the test item may be as follows:
in one possible implementation manner, there is a one-to-one mapping relationship between the test item and the reaction sample, that is, the magnetic bead method detection device may obtain the sample duty ratio according to the mapping relationship between the test item and the reaction sample. Table 3 is a mapping relationship between test items and reaction samples in an exemplary scenario. As shown in table 3, when the test item is activated partial prothrombin time, the sample ratio (i.e., the percentage of fibrinogen in the reaction sample) is thirty-three percent; and at the time of thrombin for the test item, the sample was fifty percent.
In another possible implementation, before performing a test item, a user needs to label a reaction sample label corresponding to the test item, where the reaction sample label is used to indicate a component of the reaction sample. Namely, the magnetic bead method detection device can directly obtain the percentage of the fibrinogen in the reaction sample from the reaction sample label.
403. A driving parameter is determined from the sample duty cycle.
After the magnetic bead method detection device obtains the sample duty ratio, determining a target driving voltage according to the mapping relation between the sample duty ratio and the driving voltage of the driving coil, and using the target driving voltage as the driving parameter.
In this embodiment, the mapping relationship between the sample duty ratio and the driving voltage of the driving coil is:the method comprises the steps of carrying out a first treatment on the surface of the Wherein, the->For the driving voltage, the ∈>For the sample duty cycle, the k and b are fitting coefficients. Where k and b are determined primarily by the bottom arc of the test cup, and in this embodiment k=0.50 and b=2.55 in volts (V) can be set by a number of experiments. In this embodiment, according to the mapping relationship, when the sample occupation of the reaction sample is relatively low, the driving voltage needs to be correspondingly reduced; when the sample occupancy of the reaction sample is relatively high, the driving voltage also needs to be increased correspondingly. Therefore, the frequency of waveform abnormality can be effectively reduced, the measuring range of the magnetic bead method detection device is widened, and the detection performance of magnetic bead method detection is improved.
404. Driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
After the magnetic bead method detection device acquires the driving parameter, driving the magnetic beads in the test cup according to the driving parameter, so as to detect the reaction end point of the reaction sample.
In the embodiment, when the reaction sample is detected by the magnetic bead method, the driving voltage of the magnetic bead method detection device is determined according to the sample ratio of fibrinogen in the reaction sample, so that different reaction samples are ensured to have corresponding driving voltages, the abnormal frequency of the measurement waveform is effectively reduced, the measurement range of the magnetic bead method detection device is widened, and the testing performance of the magnetic bead method test is improved.
The following describes the magnetic bead detection method in detail with an application scenario of adjusting driving parameters according to the installation levelness of the magnetic bead detection device, please refer to fig. 5, and the method is applied to the magnetic bead detection device 100. The embodiment of the magnetic bead method detection method comprises the following steps:
501. and acquiring a gravity component and a buoyancy component of the magnetic beads in the horizontal direction.
In this embodiment, the magnetic bead detection device may acquire the gravity component and the buoyancy component of the magnetic bead in the horizontal direction in the following ways:
In one possible implementation manner, the magnetic bead method detection device obtains an included angle between magnetic force born by the magnetic beads and the horizontal direction according to the levelness sensor; and then determining the gravity component and the buoyancy component of the magnetic bead in the horizontal direction according to the included angle. In this embodiment, after the magnetic force applied to the magnetic bead and the included angle of the horizontal direction are obtained, the magnetic bead detection device performs a force analysis on the magnetic bead with the horizontal direction as a reference, and obtains a gravity component and a buoyancy component of the magnetic bead in the horizontal direction according to the force analysis.
In another possible implementation manner, the magnetic bead method detection device obtains an included angle between the magnetic force applied to the magnetic bead and the horizontal direction according to at least two position sensors; and then determining the gravity component and the buoyancy component of the magnetic bead in the horizontal direction according to the included angle. In this embodiment, the number of the position sensors may be two or more, and the position sensors are mainly used for determining the included angle between the magnetic force applied to the magnetic beads and the horizontal direction according to the levelness of different parts of the magnetic bead detection device.
502. And determining the driving parameter adjustment amount according to the gravity component and the buoyancy component.
After the gravity component and the buoyancy component are acquired, the magnetic bead method detection device determines the driving parameter adjustment quantity according to the corresponding mapping relation. In this embodiment, the driving parameter adjustment amount includes at least one of a driving voltage adjustment amount and a driving duty adjustment amount. In the implementation process, the adjustment of the driving voltage may be a main means and the adjustment of the driving duty ratio may be an auxiliary means. The magnetic force born by the magnetic beads can be adjusted in a larger range by adjusting the driving voltage, and then the driving duty ratio is increased to adjust, so that the result of parameter adjustment can be optimized, and the testing performance of the magnetic bead method is ensured.
When the driving parameter adjustment amount is a driving voltage adjustment amount, the specific technical scheme may be as follows: the magnetic bead method detectsThe device determines the magnetic force adjustment quantity by utilizing a first formula according to the gravity component and the buoyancy component; the first formula is:the method comprises the steps of carrying out a first treatment on the surface of the Wherein said->For the magnetic force adjustment amount, G is the gravity force exerted by the magnetic beads, and +.>For the buoyancy force exerted by said magnetic beads, said +.>An included angle between the magnetic force applied to the magnetic beads and the horizontal direction; and then determining the driving voltage adjustment amount according to the secondary positive correlation between the magnetic force adjustment amount and the driving voltage.
When the driving parameter adjustment amount is a driving duty ratio adjustment amount, the specific technical scheme may be as follows: the magnetic bead method detection device determines the driving duty ratio adjustment quantity by utilizing a second formula according to the gravity component and the buoyancy component; the second formula is:the method comprises the steps of carrying out a first treatment on the surface of the Wherein said->For the driving duty adjustment amount, G is the gravity force to which the magnetic beads are subjected, the +.>For the buoyancy force exerted by said magnetic beads, said +.>For the magnetic force to which the magnetic beads are subjected, said +.>The included angle between the magnetic force applied to the magnetic beads and the horizontal direction.
When the driving parameter adjustment amount is the driving voltage adjustment amount and the driving duty ratio adjustment amount, the specific technical scheme is as follows:
The magnetic bead method detection device determines the magnetic force adjustment quantity by utilizing a first formula according to the gravity component and the buoyancy component; the first formula is:the method comprises the steps of carrying out a first treatment on the surface of the Wherein said->For the magnetic force adjustment amount, G is the gravity force exerted by the magnetic beads, and +.>For the buoyancy force exerted by said magnetic beads, said +.>An included angle between the magnetic force applied to the magnetic beads and the horizontal direction; then determining the driving voltage adjustment amount according to the secondary positive correlation between the magnetic force adjustment amount and the driving voltage; the magnetic bead method detection device determines the driving duty ratio adjustment quantity by utilizing a second formula according to the gravity component and the buoyancy component; the second formula is: />The method comprises the steps of carrying out a first treatment on the surface of the Wherein said->For the driving duty adjustment amount, G is the gravity force to which the magnetic beads are subjected, the +.>For the buoyancy force exerted by said magnetic beads, said +.>For the magnetic force to which the magnetic beads are subjected, said +.>The included angle between the magnetic force applied to the magnetic beads and the horizontal direction.
In this embodiment, the second formula has the same sign as the first formula. That is, when the magnetic force adjustment amount is positive (magnetic force increases), the driving duty adjustment amount is also positive (duty increases).
503. And determining the driving parameters according to the driving parameter adjustment quantity.
After the magnetic bead method detection device obtains the driving parameter adjustment quantity, the driving parameter of the original driving coil is adjusted to obtain the driving parameter. In this embodiment, when the driving parameter adjustment amount is a driving voltage adjustment amount, the driving voltage applied to the driving coil may be adjusted; when the driving parameter adjustment amount is a driving duty ratio adjustment amount, the proportion of the electrifying time of the electromagnet in the driving coil to the whole driving period can be adjusted; when the driving parameter adjustment amount is a driving voltage adjustment amount and a driving duty ratio adjustment amount, the driving voltage loaded on the driving coil can be adjusted, and the proportion of the electrifying time of the electromagnet in the driving coil to the whole driving period is adjusted on the basis of the driving voltage adjustment. 504. And driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
After the magnetic bead method detection device acquires the driving parameter, driving the magnetic beads in the test cup according to the driving parameter, so as to detect the reaction end point of the reaction sample.
In this embodiment, the magnetic bead method detection device determines the magnetic force adjustment amount to be adjusted according to the gravity component and the buoyancy component of the magnetic bead in the horizontal direction, so as to adjust the driving voltage loaded on the driving coil or the driving duty ratio of the driving coil, so that the gravity component and the buoyancy component can be ensured to be equivalent to the magnetic force, thereby enabling the magnetic bead to swing normally and improving the detection performance of the magnetic bead method detection.
It will be appreciated that the magnetic bead method shown in fig. 4 and the magnetic bead method shown in fig. 5 may be used separately or in combination, and the specific manner is not limited herein.
The method for detecting the magnetic bead method in the embodiment of the present application is described above, and the device for detecting the magnetic bead method in the embodiment of the present application is described below.
Referring specifically to fig. 6, in an embodiment of the present application, the magnetic bead detection apparatus 600 includes: an input module 601, a processing module 602, and an output module 603. The device 600 may be a magnetic bead detection device in the above method embodiments, or may be one or more chips in a magnetic bead detection device. The apparatus 600 may be used to perform some or all of the functions of the magnetic bead assay detection apparatus in the method embodiments described above.
For example, the input module 601 may be used to perform step 401 in the method embodiment described above, or to perform step 501 in the method embodiment described above. For example, the input module 601 obtains test items of a reaction sample.
The processing module 602 may be configured to perform steps 402 to 404 in the above-described method embodiments, or may be configured to perform steps 502 to 504. For example, the processing module 602 determines a sample ratio from the test item, the sample ratio representing a percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; determining a driving parameter according to the sample duty ratio; driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
The output module 603 may be configured to output the reaction endpoint or output a test waveform diagram during the test.
Optionally, the apparatus 600 further includes a storage module coupled to the processing module, so that the processing module can execute the computer-executable instructions stored in the storage module to implement the functions of the magnetic bead method detection apparatus in the above method embodiment. In one example, the memory module optionally included in the apparatus 600 may be a memory unit within a chip, such as a register, a cache, etc., and may also be a memory unit located outside the chip, such as a read-only memory (ROM) or other type of static storage device that may store static information and instructions, a random access memory (random access memory, RAM), etc.
It should be understood that the flow executed between the modules of the magnetic bead detection apparatus in the corresponding embodiment of fig. 6 is similar to the flow executed by the magnetic bead detection apparatus in the corresponding method embodiment of fig. 4 to 5, and detailed descriptions thereof are omitted herein.
Fig. 7 shows a schematic diagram of a possible structure of a magnetic bead detection device 700 according to the above embodiment, and the device 700 may be configured as the magnetic bead detection device. The apparatus 700 may include: a processor 702, computer-readable storage media/memory 703, a transceiver 704, input devices 705 and output devices 706, and a bus 701. Wherein the processor, transceiver, computer readable storage medium, etc. are connected by a bus. The embodiments of the present application are not limited to the specific connection media between the components described above.
In one example, the input device 705 obtains test items for a reaction sample;
the processor 702 determines a sample ratio from the test item, the sample ratio representing a percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; determining a driving parameter according to the sample duty ratio; driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
In one example, the processor 702 may include baseband circuitry, e.g., may generate drive parameters.
In yet another example, the processor 702 may run an operating system that controls functions between various devices and means. The transceiver 704 may include baseband circuitry and radio frequency circuitry.
The input device 705, the output device 706 and the processor 702 may implement the corresponding steps in any of the embodiments of fig. 4 to 5, and detailed descriptions thereof are omitted herein.
It will be appreciated that fig. 7 only shows a simplified design of the magnetic bead detection device, and in practical applications, the magnetic bead detection device may include any number of transceivers, processors, memories, etc., and all magnetic bead detection devices that can implement the present application are within the scope of protection of the present application.
The processor 702 in the apparatus 700 may be a general-purpose processor, such as a general-purpose Central Processing Unit (CPU), a network processor (network processor, NP), a microprocessor, etc., or may be an application-specific integrated circuit (ASIC), or one or more integrated circuits for controlling the execution of programs in the present application. But also digital signal processors (digital signal processor, DSP), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components. The controller/processor may also be a combination that performs the function of a computation, e.g., a combination comprising one or more microprocessors, a combination of a DSP and a microprocessor, etc. Processors typically perform logical and arithmetic operations based on program instructions stored in memory.
The bus 701 referred to above may be a peripheral component interconnect standard (peripheral component interconnect, PCI) bus, or an extended industry standard architecture (extended industry standard architecture, EISA) bus, or the like. The bus may be classified as an address bus, a data bus, a control bus, etc. For ease of illustration, only one thick line is shown in fig. 7, but not only one bus or one type of bus.
The computer-readable storage media/memory 703 referred to above may also hold an operating system and other application programs. In particular, the program may include program code including computer-operating instructions. More specifically, the memory may be a read-only memory (ROM), other types of static storage devices that can store static information and instructions, random access memory (random access memory, RAM), other types of dynamic storage devices that can store information and instructions, disk storage, and the like. The memory 703 may be a combination of the above memory types. And the computer readable storage medium/memory described above may be in the processor, or may be external to the processor, or distributed across multiple entities including the processor or processing circuitry. The above-described computer-readable storage medium/memory may be embodied in a computer program product. For example, the computer program product may include a computer readable medium in a packaging material.
Alternatively, embodiments of the present application also provide a general purpose processing system, such as a so-called chip, comprising one or more microprocessors that provide processor functionality, and an external memory that provides at least a portion of the storage medium, all of which are coupled to other support circuits via an external bus architecture. The instructions stored by the memory, when executed by the processor, cause the processor to perform some or all of the steps of the bead detection apparatus in the bead detection method in the embodiments described in fig. 4-5, such as steps 402-404 in fig. 4, steps 502-504 in fig. 5, and/or other processes for the techniques described herein.
The steps of a method or algorithm described in connection with the disclosure herein may be embodied in hardware, or may be embodied in software instructions executed by a processor. The software instructions may be comprised of corresponding software modules that may be stored in RAM memory, flash memory, ROM memory, EPROM memory, EEPROM memory, registers, hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art. An exemplary storage medium is coupled to the processor such the processor can read information from, and write information to, the storage medium. In the alternative, the storage medium may be integral to the processor. The processor and the storage medium may reside in an ASIC. In addition, the ASIC may reside in a user device. The processor and the storage medium may reside as discrete components in a user device.
Referring specifically to fig. 8, in an embodiment of the present application, the magnetic bead detection apparatus 800 includes: an input module 801, a processing module 802, and an output module 803. The device 800 may be a magnetic bead detection device in the above method embodiment, or may be one or more chips in a magnetic bead detection device. The apparatus 800 may be used to perform some or all of the functions of the magnetic bead assay detection apparatus in the method embodiments described above.
For example, the input module 801 may be used to perform step 501 in the method embodiments described above. For example, the input module 801 obtains a gravitational component and a buoyancy component of the magnetic beads in the horizontal direction.
The processing module 802 may be configured to perform steps 502 to 504 in the above-described method embodiments. For example, the processing module 802 determines a driving parameter adjustment based on the gravitational component and the buoyancy component; determining a driving parameter according to the driving parameter adjustment amount; driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
The output module 803 may be configured to output the reaction endpoint or output a test waveform diagram during the test.
Optionally, the apparatus 800 further includes a memory module coupled to the processing module, such that the processing module can execute the computer-executable instructions stored in the memory module to implement the functions of the magnetic bead method detection apparatus in the above method embodiment. In one example, the memory module optionally included in apparatus 800 may be an on-chip memory unit, such as a register, a cache, etc., and may also be an off-chip memory unit, such as a read-only memory (ROM) or other type of static storage device that may store static information and instructions, a random access memory (random access memory, RAM), etc.
It should be understood that the flow executed between the modules of the magnetic bead detection apparatus in the corresponding embodiment of fig. 8 is similar to the flow executed by the magnetic bead detection apparatus in the corresponding embodiment of fig. 5, and detailed descriptions thereof are omitted herein.
Fig. 9 shows a schematic diagram of a possible structure of a magnetic bead detection apparatus 900 according to the above embodiment, and the apparatus 900 may be configured as the magnetic bead detection apparatus described above. The apparatus 900 may include: a processor 902, a computer-readable storage medium/memory 903, a transceiver 904, input devices 905 and output devices 908, and a bus 901. Wherein the processor, transceiver, computer readable storage medium, etc. are connected by a bus. The embodiments of the present application are not limited to the specific connection media between the components described above.
In one example, the input device 905 acquires a gravity component and a buoyancy component of the magnetic beads in a horizontal direction;
the processor 902 determines a driving parameter adjustment based on the gravity component and the buoyancy component; determining a driving parameter according to the driving parameter adjustment amount; driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
In one example, the processor 902 may include baseband circuitry, e.g., may generate drive parameters.
In yet another example, the processor 902 may run an operating system that controls functions between various devices and means. The transceiver 904 may include baseband circuitry and radio frequency circuitry.
The input device 905, the output device 908 and the processor 902 may implement the corresponding steps in any of the embodiments of fig. 5, which are not described herein.
It will be appreciated that fig. 9 only shows a simplified design of the magnetic bead detection device, and in practical applications, the magnetic bead detection device may include any number of transceivers, processors, memories, etc., and all magnetic bead detection devices that can implement the present application are within the scope of protection of the present application.
The processor 902 referred to in the above-mentioned apparatus 900 may be a general-purpose processor, such as a general-purpose Central Processing Unit (CPU), a network processor (network processor, NP), a microprocessor, etc., or may be an application-specific integrated circuit (ASIC), or one or more integrated circuits for controlling the execution of programs in the present application. But also digital signal processors (digital signal processor, DSP), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components. The controller/processor may also be a combination that performs the function of a computation, e.g., a combination comprising one or more microprocessors, a combination of a DSP and a microprocessor, etc. Processors typically perform logical and arithmetic operations based on program instructions stored in memory.
The bus 901 referred to above may be a peripheral component interconnect standard (peripheral component interconnect, PCI) bus, or an extended industry standard architecture (extended industry standard architecture, EISA) bus, or the like. The bus may be classified as an address bus, a data bus, a control bus, etc. For ease of illustration, only one thick line is shown in fig. 9, but not only one bus or one type of bus.
The computer-readable storage media/memory 903 referred to above may also hold an operating system and other application programs. In particular, the program may include program code including computer-operating instructions. More specifically, the memory may be a read-only memory (ROM), other types of static storage devices that can store static information and instructions, random access memory (random access memory, RAM), other types of dynamic storage devices that can store information and instructions, disk storage, and the like. The memory 903 may be a combination of the above storage types. And the computer readable storage medium/memory described above may be in the processor, or may be external to the processor, or distributed across multiple entities including the processor or processing circuitry. The above-described computer-readable storage medium/memory may be embodied in a computer program product. For example, the computer program product may include a computer readable medium in a packaging material.
Alternatively, embodiments of the present application also provide a general purpose processing system, such as a so-called chip, comprising one or more microprocessors that provide processor functionality, and an external memory that provides at least a portion of the storage medium, all of which are coupled to other support circuits via an external bus architecture. The instructions stored by the memory, when executed by the processor, cause the processor to perform some or all of the steps of the bead detection apparatus in the bead detection method of the embodiment described in fig. 5, such as steps 502-504 in fig. 5, and/or other processes for the techniques described herein.
The steps of a method or algorithm described in connection with the disclosure herein may be embodied in hardware, or may be embodied in software instructions executed by a processor. The software instructions may be comprised of corresponding software modules that may be stored in RAM memory, flash memory, ROM memory, EPROM memory, EEPROM memory, registers, hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art. An exemplary storage medium is coupled to the processor such the processor can read information from, and write information to, the storage medium. In the alternative, the storage medium may be integral to the processor. The processor and the storage medium may reside in an ASIC. In addition, the ASIC may reside in a user device. The processor and the storage medium may reside as discrete components in a user device.
It will be clear to those skilled in the art that, for convenience and brevity of description, specific working procedures of the above-described systems, apparatuses and units may refer to corresponding procedures in the foregoing method embodiments, which are not repeated herein.
In the several embodiments provided in this application, it should be understood that the disclosed systems, apparatuses, and methods may be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative, e.g., the division of the units is merely a logical function division, and there may be additional divisions when actually implemented, e.g., multiple units or components may be combined or integrated into another system, or some features may be omitted or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with each other may be an indirect coupling or communication connection via some interfaces, devices or units, which may be in electrical, mechanical or other form.
The units described as separate units may or may not be physically separate, and units shown as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
In addition, each functional unit in each embodiment of the present application may be integrated in one processing unit, or each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units may be implemented in hardware or in software functional units.
The integrated units, if implemented in the form of software functional units and sold or used as stand-alone products, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present application may be embodied in essence or a part contributing to the prior art or all or part of the technical solution in the form of a software product stored in a storage medium, including several instructions to cause a computer device (which may be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the methods described in the embodiments of the present application. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a random access Memory (RAM, random Access Memory), a magnetic disk, or an optical disk, or other various media capable of storing program codes.
The above embodiments are merely for illustrating the technical solution of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (16)
1. A method for detecting a magnetic bead, comprising:
obtaining a test item of a reaction sample, the test item comprising: coagulation function analysis or anticoagulation function analysis;
determining a sample ratio according to the test item, wherein the sample ratio is used for representing the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample;
determining a driving parameter according to the sample duty ratio;
driving the magnetic beads according to the driving parameters, and detecting a reaction end point of the reaction sample;
acquiring a gravity component and a buoyancy component of the magnetic beads in the horizontal direction;
determining the driving parameter adjustment amount according to the gravity component and the buoyancy component comprises: determining a magnetic force adjustment amount according to the gravity component and the buoyancy component; determining the driving voltage adjustment amount according to the secondary positive correlation between the magnetic force adjustment amount and the driving voltage;
And determining the driving parameters according to the driving parameter adjustment quantity.
2. The method of claim 1, wherein determining a drive parameter from the sample duty cycle comprises:
and determining a target driving voltage according to the mapping relation between the sample duty ratio and the driving voltage, wherein the target driving voltage is used as the driving parameter.
3. The method of claim 2, wherein the mapping of the sample duty cycle to the drive voltage is:；
wherein the saidFor the driving voltage, the +.>For the sample duty cycle, said +.>And->Is the fitting coefficient.
4. A method according to any one of claims 1 to 3, wherein said determining a sample duty cycle from said test item comprises:
the sample ratio is obtained from a reaction sample label corresponding to the test item, wherein the reaction sample label is used for indicating the composition of the reaction sample;
or alternatively, the first and second heat exchangers may be,
obtaining a mapping relation between the test item and the sample duty ratio;
and determining the sample duty ratio according to the mapping relation between the test item and the sample duty ratio.
5. The method of claim 1, wherein said determining a magnetic force adjustment from the gravitational component and the buoyancy component comprises:
Determining the magnetic force adjustment amount by using a first formula according to the gravity component and the buoyancy component;
the first formula is:
；
wherein the saidFor the magnetic force adjustment, the +.>For the weight force to which the magnetic beads are subjected, said +.>For the buoyancy force exerted by said magnetic beads, said +.>The included angle between the magnetic force applied to the magnetic beads and the horizontal direction.
6. The method of claim 1, wherein determining a drive parameter adjustment from the gravitational component and the buoyancy component comprises:
determining a magnetic force adjustment amount according to the gravity component and the buoyancy component, and determining the driving voltage adjustment amount according to a secondary positive correlation between the magnetic force adjustment amount and the driving voltage;
and determining a driving duty ratio adjustment amount according to the gravity component and the buoyancy component, wherein the driving voltage adjustment amount and the driving duty ratio adjustment amount serve as the driving parameter adjustment amount.
7. The method of claim 6, wherein determining a drive duty cycle adjustment from the gravitational component and the buoyancy component comprises:
determining a driving duty ratio adjustment amount by using a second formula according to the gravity component and the buoyancy component;
the second formula is:
；
Wherein the saidFor the driving duty adjustment amount, said +.>For the weight force to which the magnetic beads are subjected, said +.>For the buoyancy force exerted by said magnetic beads, said +.>For the magnetic force to which the magnetic beads are subjected, said +.>For the magnetic beadsThe included angle between the magnetic force and the horizontal direction.
8. The method of any one of claims 5 to 7, wherein the acquiring the gravitational and buoyancy components of the magnetic beads comprises:
acquiring an included angle between the magnetic force born by the magnetic beads and the horizontal direction according to a levelness sensor;
determining a gravity component and a buoyancy component of the magnetic beads in the horizontal direction according to the included angle;
or alternatively, the first and second heat exchangers may be,
acquiring an included angle between the magnetic force born by the magnetic beads and the horizontal direction according to at least two position sensors;
and determining a gravity component and a buoyancy component of the magnetic beads in the horizontal direction according to the included angle.
9. A magnetic bead method detection apparatus, comprising: input device, processor, output device;
the input device is used for acquiring test items of a reaction sample, and the test items comprise: coagulation function analysis or anticoagulation function analysis;
the processor is used for determining a sample ratio according to the test item, wherein the sample ratio is used for representing the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; determining a driving parameter according to the sample duty ratio; driving the magnetic beads according to the driving parameters, and detecting a reaction end point of the reaction sample;
The input device is also used for acquiring a gravity component and a buoyancy component of the magnetic beads;
the processor is also used for determining a driving parameter adjustment amount according to the gravity component and the buoyancy component; determining the driving parameters according to the driving parameter adjustment quantity;
the processor is specifically used for determining a magnetic force adjustment amount according to the gravity component and the buoyancy component; determining the driving voltage adjustment amount according to the secondary positive correlation between the magnetic force adjustment amount and the driving voltage;
the output device is used for outputting the reaction end point.
10. The apparatus according to claim 9, wherein the processor is configured to determine a target driving voltage as the driving parameter according to a mapping between the sample duty ratio and the driving voltage.
11. The apparatus of claim 10, wherein the sample duty cycle versus drive voltage mapping is:；
wherein the saidFor the driving voltage, the +.>For the sample duty cycle, said +.>And->Is the fitting coefficient.
12. The device according to any one of claims 9 to 11, wherein the processor is configured to obtain the sample duty cycle from a reaction sample label corresponding to the test item, the reaction sample label being configured to indicate a composition of the reaction sample;
Or alternatively, the first and second heat exchangers may be,
obtaining a mapping relation between the test item and the sample duty ratio;
and determining the sample duty ratio according to the mapping relation between the test item and the sample duty ratio.
13. The apparatus of claim 9, wherein the processor is configured to determine the magnetic force adjustment amount using a first formula based on the gravitational component and the buoyancy component;
the first formula is:
；
wherein the saidFor the magnetic force adjustment, the +.>For the weight force to which the magnetic beads are subjected, said +.>For the buoyancy force exerted by said magnetic beads, said +.>The included angle between the magnetic force applied to the magnetic beads and the horizontal direction.
14. The device according to claim 9, wherein the processor is specifically configured to determine a magnetic force adjustment amount according to the gravity component and the buoyancy component, and determine the driving voltage adjustment amount according to a quadratic positive correlation between the magnetic force adjustment amount and the driving voltage;
and determining a driving duty ratio adjustment amount according to the gravity component and the buoyancy component, wherein the driving voltage adjustment amount and the driving duty ratio adjustment amount serve as the driving parameter adjustment amount.
15. The apparatus of claim 14, wherein the processor is configured to determine a driving duty cycle adjustment from the gravitational component and the buoyancy component using a second formula;
The second formula is:
；
wherein the saidFor the driving duty adjustment amount, said +.>For the weight force to which the magnetic beads are subjected, said +.>For the buoyancy force exerted by said magnetic beads, said +.>For the magnetic force to which the magnetic beads are subjected, said +.>The included angle between the magnetic force applied to the magnetic beads and the horizontal direction.
16. The apparatus according to any one of claims 13 to 15, wherein the input device is specifically configured to obtain, according to a levelness sensor, an angle between a magnetic force applied to the magnetic bead and a horizontal direction;
determining a gravity component and a buoyancy component of the magnetic beads in the horizontal direction according to the included angle;
or alternatively, the first and second heat exchangers may be,
acquiring an included angle between the magnetic force born by the magnetic beads and the horizontal direction according to at least two position sensors;
and determining a gravity component and a buoyancy component of the magnetic beads in the horizontal direction according to the included angle.
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Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4317363A (en) * | 1980-06-16 | 1982-03-02 | Abbott Laboratories | Elastomer instrument |
| US4918984A (en) * | 1987-12-30 | 1990-04-24 | Serbio | Device for measuring the modification time of the physical state of a fluid medium |
| DE20014891U1 (en) * | 2000-08-29 | 2001-03-01 | Beiersdorf Ag, 20253 Hamburg | Measuring device for determining the viscoelastic properties of PSAs, especially in thin layers |
| CN102262090A (en) * | 2010-05-24 | 2011-11-30 | 北京普利生仪器有限公司 | Method and equipment for measuring platelet aggregation and blood coagulation factor |
| CN102565317A (en) * | 2010-12-24 | 2012-07-11 | 北京普利生仪器有限公司 | Blood coagulation factor and fibrinolysis measuring method |
| CN202974866U (en) * | 2012-10-26 | 2013-06-05 | 北京众驰伟业科技发展有限公司 | Blood coagulation tester core adopting double methodologies |
| CN103140291A (en) * | 2010-07-09 | 2013-06-05 | 超微生物控股有限公司 | Low volume assay apparatus |
| CN104458503A (en) * | 2014-12-12 | 2015-03-25 | 广州阳普医疗科技股份有限公司 | Blood coagulation detection instrument |
| CN105548582A (en) * | 2014-10-31 | 2016-05-04 | 陕西盛迈石油有限公司 | Blood coagulation analyzer |
| WO2017004368A1 (en) * | 2015-07-02 | 2017-01-05 | U.S. Government As Represented By The Secretary Of The Army | Thromboelastograph-based decision support system |
| CN107003297A (en) * | 2014-12-15 | 2017-08-01 | 斯塔戈诊断公司 | For the method and apparatus for the setting time for determining blood sample, and reaction vessel |
| JP2017198594A (en) * | 2016-04-28 | 2017-11-02 | シスメックス株式会社 | Blood specimen analytic method, reagent and reagent kit for blood specimen analysis, and blood specimen analyzer |
| CN108709906A (en) * | 2018-06-28 | 2018-10-26 | 西安交通大学 | A kind of magnetic control shape memory alloy material drive characteristic test platform and test method |
| CN108872619A (en) * | 2017-04-24 | 2018-11-23 | 希森美康株式会社 | The analysis method and analytical equipment of blood sample |
| CN109395625A (en) * | 2017-08-16 | 2019-03-01 | 北京普利生仪器有限公司 | A kind of stirring means and mechanism, vitro detection equipment of reaction solution |
-
2019
- 2019-06-28 CN CN201910577939.7A patent/CN110631964B/en active Active
Patent Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4317363A (en) * | 1980-06-16 | 1982-03-02 | Abbott Laboratories | Elastomer instrument |
| US4918984A (en) * | 1987-12-30 | 1990-04-24 | Serbio | Device for measuring the modification time of the physical state of a fluid medium |
| DE20014891U1 (en) * | 2000-08-29 | 2001-03-01 | Beiersdorf Ag, 20253 Hamburg | Measuring device for determining the viscoelastic properties of PSAs, especially in thin layers |
| CN102262090A (en) * | 2010-05-24 | 2011-11-30 | 北京普利生仪器有限公司 | Method and equipment for measuring platelet aggregation and blood coagulation factor |
| CN103140291A (en) * | 2010-07-09 | 2013-06-05 | 超微生物控股有限公司 | Low volume assay apparatus |
| CN102565317A (en) * | 2010-12-24 | 2012-07-11 | 北京普利生仪器有限公司 | Blood coagulation factor and fibrinolysis measuring method |
| CN202974866U (en) * | 2012-10-26 | 2013-06-05 | 北京众驰伟业科技发展有限公司 | Blood coagulation tester core adopting double methodologies |
| CN105548582A (en) * | 2014-10-31 | 2016-05-04 | 陕西盛迈石油有限公司 | Blood coagulation analyzer |
| CN104458503A (en) * | 2014-12-12 | 2015-03-25 | 广州阳普医疗科技股份有限公司 | Blood coagulation detection instrument |
| CN107003297A (en) * | 2014-12-15 | 2017-08-01 | 斯塔戈诊断公司 | For the method and apparatus for the setting time for determining blood sample, and reaction vessel |
| WO2017004368A1 (en) * | 2015-07-02 | 2017-01-05 | U.S. Government As Represented By The Secretary Of The Army | Thromboelastograph-based decision support system |
| JP2017198594A (en) * | 2016-04-28 | 2017-11-02 | シスメックス株式会社 | Blood specimen analytic method, reagent and reagent kit for blood specimen analysis, and blood specimen analyzer |
| CN107340272A (en) * | 2016-04-28 | 2017-11-10 | 希森美康株式会社 | For analyzing method, reagent and the kit and device of blood subject |
| CN108872619A (en) * | 2017-04-24 | 2018-11-23 | 希森美康株式会社 | The analysis method and analytical equipment of blood sample |
| CN109395625A (en) * | 2017-08-16 | 2019-03-01 | 北京普利生仪器有限公司 | A kind of stirring means and mechanism, vitro detection equipment of reaction solution |
| CN108709906A (en) * | 2018-06-28 | 2018-10-26 | 西安交通大学 | A kind of magnetic control shape memory alloy material drive characteristic test platform and test method |
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| Publication number | Publication date |
|---|---|
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