CN110631964A - Magnetic bead method detection method and magnetic bead method detection device - Google Patents
Magnetic bead method detection method and magnetic bead method detection device Download PDFInfo
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- G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
- G01N11/10—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by moving a body within the material
- G01N11/16—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by moving a body within the material by measuring damping effect upon oscillatory body
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Abstract
The embodiment of the application provides a magnetic bead method detection method and a magnetic bead detection device, which are used for improving the performance of a magnetic bead method test. The method in the embodiment of the application comprises the following steps: obtaining a test item of a reaction sample; determining a sample proportion according to the test item, wherein the sample proportion is used for representing the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; determining a driving parameter according to the sample ratio; and driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
Description
Technical Field
The application relates to the field of medical instruments, in particular to a magnetic bead method detection method and a magnetic bead detection device.
Background
The blood coagulation analyzer is an apparatus which is clinically used for analyzing the functions of blood coagulation and anticoagulation, fibrinolysis and anti-fibrinolysis of blood of patients. Among them, the coagulation method can be used for measuring coagulation properties or fibrinolysis properties of blood samples. The magnetic bead method is currently used to test the coagulation characteristics of blood samples. The magnetic bead method includes a double magnetic circuit magnetic bead method, a photoelectric magnetic bead method and the like, and the reaction end point of the blood coagulation process is judged according to the viscosity characteristic change in a reaction system. The principle is as follows: a group of driving coils are arranged on two sides of the test cup and used for generating a constant alternating electromagnetic field so as to keep the special demagnetizing small steel balls in the test cup in equal-amplitude oscillating motion; and the other group of measuring coils or photoelectric sensors of the instrument can record the motion change of the small steel ball, and when the motion amplitude is attenuated to 50%, the solidification end point is judged.
The measurement process of the magnetic bead method belongs to a dynamic measurement process, so that the magnetic bead method is easily influenced by dynamic interference factors, and can cause the magnetic beads to swing on one side and cannot carry out testing in severe cases.
Disclosure of Invention
The embodiment of the application provides a magnetic bead method detection method and a magnetic bead detection device, which are used for improving the performance of a magnetic bead method test.
In a first aspect, an embodiment of the present application provides a magnetic bead method detection method, which specifically includes: obtaining a test item of a reaction sample; determining a sample proportion according to the test item, wherein the sample proportion is used for representing the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; determining a driving parameter according to the sample ratio; and driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
In a second aspect, an embodiment of the present application provides a magnetic bead method detection method, which specifically includes: acquiring a gravity component and a buoyancy component of the magnetic beads in the horizontal direction; determining a driving parameter adjustment amount according to the gravity component and the buoyancy component; determining a driving parameter according to the driving parameter adjustment amount; and driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
In a third aspect, an embodiment of the present application provides a detection device using a magnetic bead method, which specifically includes: an input device, a processor, an output device; the input equipment is used for acquiring a test item of a reaction sample; the processor is used for determining a sample proportion according to the test item, and the sample proportion is used for representing the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; determining a driving parameter according to the sample ratio; driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample; the output device is used for outputting the reaction end point.
In a fourth aspect, an embodiment of the present application provides a magnetic bead method detection device, which specifically includes: an input device, a processor, and an output device; the input device is used for acquiring a gravity component and a buoyancy component of the magnetic beads; the processor is used for determining a driving parameter adjustment amount according to the gravity component and the buoyancy component; determining a driving parameter according to the driving parameter adjustment amount; driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample; the output device is used for outputting the reaction end point.
According to the technical scheme, the embodiment of the application has the following advantages: when a reaction sample is detected by a paramagnetic particle method, the driving voltage of the detection device by the paramagnetic particle method is determined according to the sample proportion of fibrinogen in the reaction sample, so that different reaction samples are ensured to have corresponding driving voltages, the frequency of measurement waveform abnormity is effectively reduced, the measurement range of the detection device by the paramagnetic particle method is widened, and the test performance of the test by the paramagnetic particle method is improved.
Drawings
FIG. 1 is a block diagram illustrating an exemplary structure of a magnetic bead assay detection device 100 according to an embodiment of the present disclosure;
FIG. 2 is a schematic diagram of a magnetic bead method test principle in a sensor measurement model of a dual magnetic circuit magnetic bead method;
FIG. 3 is a schematic diagram of the force analysis of the magnetic beads in the test cup;
FIG. 4 is a schematic view of an embodiment of a magnetic bead method detection method in the embodiment of the present application;
FIG. 5 is a schematic view of another embodiment of a magnetic bead method detection method in the embodiment of the present application;
FIG. 6 is a schematic view of an embodiment of a magnetic bead assay detection device in an embodiment of the present application;
FIG. 7 is a schematic view of another embodiment of a magnetic bead assay detection device in an embodiment of the present application;
FIG. 8 is a schematic view of another embodiment of a magnetic bead assay detection device in an embodiment of the present application;
FIG. 9 is a schematic view of another embodiment of a magnetic bead assay detection device in an embodiment of the present application.
Detailed Description
The embodiment of the application provides a magnetic bead method detection method and a magnetic bead method detection device, which are used for improving the performance of a magnetic bead method test.
The terms "first," "second," "third," "fourth," and the like in the description and in the claims of the present application and in the drawings described above, if any, are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It will be appreciated that the data so used may be interchanged under appropriate circumstances such that the embodiments described herein may be practiced otherwise than as specifically illustrated or described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
Fig. 1 is a schematic diagram illustrating an exemplary structure of a magnetic bead assay detection device 100 in an embodiment of the present application. The magnetic bead method detection apparatus 100 includes an input device 101, a processor 102, and an output device 103. The input device 101 may be used to obtain the test items of the reaction samples, that is, the user may directly input the test items of the reaction samples through the input device 101; the processor 102 determines a sample proportion of fibrinogen in the reaction sample according to the test item, wherein the sample proportion is used for representing a percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; the processor 102 then determines the drive parameters based on the sample ratios; driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample; finally, the reaction end point is outputted through the output device 103.
In this embodiment, the output device 103 of the magnetic bead method detection apparatus 100 may be a touch display screen, a liquid crystal display, or an independent display device such as a liquid crystal display and a television set, which is independent of the magnetic bead method detection apparatus 100, or a display screen on an electronic device such as a mobile phone and a tablet computer.
In an exemplary measurement model of a sensor using a dual magnetic circuit magnetic bead method as shown in fig. 2, the magnetic bead method in the embodiment of the present application is as follows: a group of driving coils are arranged on two sides of the test cup and used for generating a constant alternating electromagnetic field so that the special demagnetizing steel balls in the test cup keep equal-amplitude oscillation motion. After the blood coagulation activator is added, the viscosity of the reaction system is continuously increased along with the conversion of fibrinogen into cross-linked fibrin, the motion amplitude of the small steel ball is gradually weakened, the motion change of the small steel ball can be recorded by another group of measuring coils of the instrument, and when the motion amplitude is attenuated to fifty percent, the reaction end point is judged. And the force analysis of the magnetic beads in the test cup is shown in figure 3. In an exemplary embodiment, the specific analysis of the stress can be as shown in table 1. It is understood that the information on the mass, density, spherical radius, volume, density of the reaction system, etc. of the magnetic beads in table 1 is only an exemplary scheme.
TABLE 1
According to the stress analysis, when the magnetic beads are arranged on two sides of the reaction cup, the component of the gravity of the magnetic beads on the electromagnetic force direction of the magnetic beads is arranged on the sensor installation levelness of the magnetic bead method when the working condition of the detection device of the magnetic bead method is not met, the detection result waveform is easy to be asymmetric, even the magnetic bead detection performance is influenced. Meanwhile, in the process of detection by the magnetic bead method, parameters of a driving coil are generally matched with the viscosity of a reaction sample. If the initial viscosity of the reaction sample is larger, but the driving force of the driving coil is smaller, the amplitude of the magnetic beads is quickly attenuated to about 0 value after the mixing stage is finished, the viscosity characteristic change of the reaction system cannot be monitored at all, and information such as a reaction end point and the like cannot be obtained, and the detection process fails; if the initial viscosity of the reaction sample is small, but the driving force of the driving coil is large, the magnetic beads frequently collide with the reaction cup in the detection process, so that the amplitude obtained by the measuring coil is unstable, and the problems that the magnetic beads are self-locked at one side of the test cup and cannot move are possibly caused. While the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample (i.e., the sample ratio) can represent the initial viscosity of the reaction sample, an exemplary embodiment of the relationship between the sample ratio and the initial viscosity of the reaction sample can be shown in table 2.
TABLE 2
Therefore, a user can determine the relation between the sample ratio and the driving voltage according to the relation between the sample ratio and the initial viscosity, and further adjust the driving parameters of the magnetic bead method detection device.
In order to solve the problem, the embodiment of the present application may optimize the magnetic bead method detection method in terms of the sample ratio and the installation levelness of the magnetic bead method detection device.
For convenience of understanding, the related terms are explained in the embodiments of the present application, and are specifically as follows:
driving voltage: and the voltage value is loaded on the driving coil, wherein the driving force generated by the driving coil and used for driving the magnetic beads to move is in a secondary positive correlation with the driving voltage. In the embodiment of the application, the driving coils on two sides of the test cup in the magnetic bead method detection device can be respectively and independently loaded with the driving voltage, and the size of the driving voltage can be independently adjusted.
Driving period: and the magnetic bead method detection device drives the magnetic beads to normally swing in the test cup.
Duty ratio: the electromagnet conduction time accounts for the proportion of the whole drive period.
Sample ratio: fibrinogen in the reaction sample is a percentage of the total volume of the reaction sample.
Referring to fig. 4, a detailed description is given below of a magnetic bead method detection method according to an application scenario in which a sample proportion of a reaction sample is adjusted to a drive parameter, and the method provided in the embodiment of the present application is applied to a magnetic bead method detection device 100. The embodiment of the detection method by the magnetic bead method in the application comprises the following steps:
401. and obtaining the test items of the reaction samples.
When a user detects a reaction sample, the user firstly obtains a test item of the reaction sample. The test items in this embodiment include, but are not limited to, blood coagulation function analysis, blood anticoagulation
402. Determining a sample fraction representing a percentage of fibrinogen in the reaction sample to a total volume of the reaction sample based on the test item.
In this embodiment, the mode of obtaining the sample proportion by the magnetic bead method detection device according to the test item may be as follows:
in one possible implementation manner, there is a one-to-one mapping relationship between the test item and the reaction sample, that is, the magnetic bead method detection device may obtain the sample proportion according to the mapping relationship between the test item and the reaction sample. Table 3 is a mapping between test items and reaction samples in an exemplary scenario. As shown in table 3, when the test item is the activated partial prothrombin time, the sample percentage (i.e. fibrinogen percentage of the reaction sample) is thirty-three percent; and when the test item is thrombin time, the sample proportion is fifty percent.
TABLE 3
In another possible implementation manner, before a test item is performed, a user needs to label a reaction sample corresponding to the test item with a reaction sample label, where the reaction sample label is used to indicate a component of the reaction sample. That is, the magnetic bead method detection device can directly obtain the percentage of the fibrinogen in the reaction sample from the reaction sample label.
403. The drive parameters are determined based on the sample ratio.
After the magnetic bead method detection device acquires the sample proportion, determining a target driving voltage according to the mapping relation between the sample proportion and the driving voltage of the driving coil, and using the target driving voltage as the driving parameter.
In this embodiment, the mapping relationship between the sample ratio and the driving voltage of the driving coil is as follows: vmag＝klg(Rsample) + b; wherein, the VmagFor the driving voltage, the RsampleFor the sample fraction, k and b are fitting coefficients. Where k and b are determined primarily by the bottom arc of the test cup, and in this embodiment, k is 0.50 and b is 2.55 volts (V) through a number of experiments. In this embodiment, according to the mapping relationship, when the sample proportion of the reaction sample is low, the driving voltage also needs to be correspondingly reduced; when the sample proportion of the reaction sample is high, the driving voltage also needs to be increased correspondingly. Therefore, the frequency of waveform abnormity can be effectively reduced, the measurement range of the magnetic bead method detection device is widened, and the detection performance of the magnetic bead method detection is improved.
404. And driving the magnetic beads according to the driving parameters, and detecting the reaction endpoint of the reaction sample.
After the magnetic bead method detection device obtains the driving parameter, the magnetic beads in the test cup are driven according to the driving parameter, so that the reaction end point of the reaction sample is detected.
In this embodiment, when a reaction sample is detected by a paramagnetic particle method, the driving voltage of the paramagnetic particle method detection device is determined according to the sample ratio of fibrinogen in the reaction sample, so as to ensure that different reaction samples have corresponding driving voltages, effectively reduce the frequency of abnormal measurement waveforms, widen the measurement range of the paramagnetic particle method detection device, and improve the test performance of the paramagnetic particle method test.
The magnetic bead method detection method in the present application is described in detail in an application scenario in which the driving parameter is adjusted according to the installation levelness of the magnetic bead method detection device, and please refer to fig. 5, the magnetic bead method detection method provided in the embodiment of the present application is applied to the magnetic bead method detection device 100. The embodiment of the detection method by the magnetic bead method in the application comprises the following steps:
501. and acquiring the gravity component and the buoyancy component of the magnetic beads in the horizontal direction.
In this embodiment, the magnetic bead method detection device may obtain the gravity component and the buoyancy component of the magnetic bead in the horizontal direction by using the following several methods:
in one possible implementation mode, the magnetic bead method detection device obtains an included angle between the magnetic force applied to the magnetic bead and the horizontal direction according to the levelness sensor; and then determining the gravity component and the buoyancy component of the magnetic bead in the horizontal direction according to the included angle. In this embodiment, after the magnetic bead detection device obtains the included angle between the magnetic force applied to the magnetic bead and the horizontal direction, the horizontal direction is used as a reference to perform stress analysis on the magnetic bead, and the gravity component and the buoyancy component of the magnetic bead in the horizontal direction are obtained according to the stress analysis.
In another possible implementation manner, the magnetic bead method detection device obtains an included angle between the magnetic force borne by the magnetic bead and the horizontal direction according to at least two position sensors; and then determining the gravity component and the buoyancy component of the magnetic bead in the horizontal direction according to the included angle. In this embodiment, the number of the position sensors may be two or more, and the position sensors are mainly used for determining an included angle between the magnetic force applied to the magnetic bead and the horizontal direction according to levelness of different parts of the magnetic bead method detection device.
502. And determining the adjustment amount of the driving parameter according to the gravity component and the buoyancy component.
After the gravity component and the buoyancy component are obtained, the magnetic bead method detection device determines the adjustment amount of the driving parameters according to the corresponding mapping relation. In this embodiment, the driving parameter adjustment amount includes at least one of a driving voltage adjustment amount and a driving duty adjustment amount. In a specific implementation process, the driving voltage can be adjusted as a main means, and the driving duty ratio can be adjusted as an auxiliary means. The magnetic force borne by the magnetic beads can be adjusted within a large range by adjusting the driving voltage, and then the adjustment of the driving duty ratio is increased, so that the result of parameter adjustment can be optimized, and the testing performance of the magnetic bead method is ensured.
When the driving parameter adjustment amount is a driving voltage adjustment amount, the specific technical scheme may be as follows: the magnetic bead method detection device determines the magnetic force adjustment quantity by utilizing a first formula according to the gravity component and the buoyancy component; the first formula is: Δ FM＝±(G-FB) sin beta; wherein, the Δ FMThe G is the gravity borne by the magnetic beads, and the F is the magnetic force adjustment amountBThe beta is the included angle between the magnetic force applied to the magnetic beads and the horizontal direction; and then determining the driving voltage adjustment amount according to the secondary positive correlation between the magnetic force adjustment amount and the driving voltage.
When the driving parameter adjustment amount is a driving duty ratio adjustment amount, the specific technical scheme may be as follows: the magnetic bead method detection device determines a driving duty ratio adjustment quantity by utilizing a second formula according to the gravity component and the buoyancy component; the second formula is:
wherein Δ t is the driving duty ratio adjustment amount, G is the gravity borne by the magnetic bead, and F is the weightBIs the buoyancy to which the magnetic beads are subjected, FMThe beta is an included angle between the magnetic force applied to the magnetic bead and the horizontal direction.
When the driving parameter adjustment amount is the driving voltage adjustment amount and the driving duty ratio adjustment amount, the specific technical scheme is as follows:
the magnetic bead method detection device determines the magnetic force adjustment quantity by utilizing a first formula according to the gravity component and the buoyancy component; the first formula is: Δ FM＝±(G-FB) sin beta; wherein, the Δ FMThe G is the gravity borne by the magnetic beads, and the F is the magnetic force adjustment amountBThe beta is the included angle between the magnetic force applied to the magnetic beads and the horizontal direction; then adjusting the amount according to the magnetic forceDetermining the driving voltage adjustment amount according to the secondary positive correlation relation with the driving voltage; the magnetic bead method detection device determines a driving duty ratio adjustment quantity by utilizing a second formula according to the gravity component and the buoyancy component; the second formula is:
wherein Δ t is the driving duty ratio adjustment amount, G is the gravity borne by the magnetic bead, and F is the weightBIs the buoyancy to which the magnetic beads are subjected, FMThe beta is an included angle between the magnetic force applied to the magnetic bead and the horizontal direction.
In this embodiment, the second formula has the same sign as the first formula. That is, when the magnetic force adjustment amount is positive (magnetic force increases), the drive duty adjustment amount is also positive (duty increases).
503. And determining the driving parameter according to the driving parameter adjustment amount.
After the magnetic bead method detection device obtains the adjustment quantity of the driving parameter, the driving parameter on the original driving coil is adjusted to obtain the driving parameter. In this embodiment, when the driving parameter adjustment amount is a driving voltage adjustment amount, the driving voltage loaded on the driving coil may be adjusted; when the drive parameter adjustment amount is the drive duty ratio adjustment amount, the proportion of the electrifying time of the electromagnet in the drive coil in the whole drive period can be adjusted; when the driving parameter adjustment amount is the driving voltage adjustment amount and the driving duty ratio adjustment amount, the driving voltage loaded on the driving coil can be adjusted, and the proportion of the electromagnet electrifying time in the driving coil to the whole driving period is adjusted on the basis of the driving voltage adjustment. 504. And driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
After the magnetic bead method detection device obtains the driving parameter, the magnetic beads in the test cup are driven according to the driving parameter, so that the reaction end point of the reaction sample is detected.
In this embodiment, this magnetic bead method detection device confirms the magnetic force adjustment volume that needs the adjustment according to this magnetic bead gravity component and buoyancy component on the horizontal direction, and then adjusts the drive voltage of loading on the drive coil or the drive duty cycle of this drive coil, can guarantee like this that this gravity component and buoyancy component can be equivalent with magnetic force to make the magnetic bead can normally realize the swing, improve the detection performance that the magnetic bead method detected.
It is understood that the magnetic bead method detection method shown in fig. 4 and the magnetic bead method detection method shown in fig. 5 may be used separately or in combination, and the specific manner is not limited herein.
The magnetic bead method detection method in the embodiment of the present application is described above, and the magnetic bead method detection device in the embodiment of the present application is described below.
Specifically, referring to fig. 6, the magnetic bead method detecting device 600 in the embodiment of the present application includes: an input module 601, a processing module 602, and an output module 603. The device 600 may be the magnetic bead assay device in the above-described method embodiments, or may be one or more chips in the magnetic bead assay device. The device 600 may be used to perform some or all of the functions of the magnetic bead assay detection device in the above-described method embodiments.
For example, the input module 601 may be configured to execute step 401 in the above-described method embodiment, or configured to execute step 501 in the foregoing method embodiment. For example, the input module 601 acquires a test item that reflects a sample.
The processing module 602 may be configured to perform steps 402 to 404 in the above method embodiment, or to perform steps 502 to 504. For example, the processing module 602 determines a sample fraction indicating a percentage of fibrinogen in the reaction sample to the total volume of the reaction sample according to the test item; determining a driving parameter according to the sample ratio; and driving the magnetic beads according to the driving parameters, and detecting the reaction endpoint of the reaction sample.
The output module 603 may be configured to output the reaction endpoint or output a test waveform during the test.
Optionally, the apparatus 600 further includes a storage module, which is coupled to the processing module, so that the processing module can execute computer-executable instructions stored in the storage module to implement the functions of the magnetic bead method detection apparatus in the above-described method embodiments. In one example, the memory module optionally included in the apparatus 600 may be a memory unit inside the chip, such as a register, a cache, or the like, and the memory module may also be a memory unit located outside the chip, such as a read-only memory (ROM) or another type of static storage device that can store static information and instructions, a Random Access Memory (RAM), or the like.
It should be understood that the flow executed between the modules of the magnetic bead method detection device in the embodiment corresponding to fig. 6 is similar to the flow executed by the magnetic bead method detection device in the embodiment corresponding to fig. 4 to fig. 5, and detailed description thereof is omitted here.
Fig. 7 is a schematic diagram illustrating a possible structure of a magnetic bead assay detection device 700 in the above embodiment, where the device 700 may be configured as the magnetic bead assay detection device. The apparatus 700 may include: a processor 702, a computer-readable storage medium/memory 703, a transceiver 704, an input device 705 and an output device 706, and a bus 701. Wherein the processor, transceiver, computer readable storage medium, etc. are connected by a bus. The embodiments of the present application do not limit the specific connection medium between the above components.
In one example, the input device 705 obtains a test item reflecting a sample;
the processor 702 determines a sample fraction representing a percentage of fibrinogen in the reaction sample to a total volume of the reaction sample based on the test item; determining a driving parameter according to the sample ratio; and driving the magnetic beads according to the driving parameters, and detecting the reaction endpoint of the reaction sample.
In one example, the processor 702 may include baseband circuitry, e.g., may generate drive parameters.
In yet another example, the processor 702 may run an operating system that controls functions between various devices and appliances. The transceiver 704 may include baseband circuitry and radio frequency circuitry.
The input device 705, the output device 706 and the processor 702 may implement corresponding steps in any one of the embodiments of fig. 4 to fig. 5, which are not described herein in detail.
It is understood that fig. 7 only shows a simplified design of the magnetic bead assay device, and in practical applications, the magnetic bead assay device may include any number of transceivers, processors, memories, etc., and all magnetic bead assay devices that can implement the present application are within the scope of the present application.
The processor 702 involved in the apparatus 700 may be a general-purpose processor, such as a general-purpose Central Processing Unit (CPU), a Network Processor (NP), a microprocessor, etc., or an application-specific integrated circuit (ASIC), or one or more integrated circuits for controlling the execution of the program according to the present application. But also a Digital Signal Processor (DSP), a field-programmable gate array (FPGA) or other programmable logic device, discrete gate or transistor logic device, discrete hardware components. The controller/processor can also be a combination of computing functions, e.g., comprising one or more microprocessors, DSPs, and microprocessors, among others. Processors typically perform logical and arithmetic operations based on program instructions stored within memory.
The bus 701 may be a Peripheral Component Interconnect (PCI) bus, an Extended Industry Standard Architecture (EISA) bus, or the like. The bus may be divided into an address bus, a data bus, a control bus, etc. For ease of illustration, only one thick line is shown in FIG. 7, but this is not intended to represent only one bus or type of bus.
The computer-readable storage medium/memory 703 referred to above may also hold an operating system and other application programs. In particular, the program may include program code including computer operating instructions. More specifically, the memory may be a read-only memory (ROM), other types of static storage devices that may store static information and instructions, a Random Access Memory (RAM), other types of dynamic storage devices that may store information and instructions, a disk memory, and so forth. The memory 703 may be a combination of the above memory types. And the computer-readable storage medium/memory described above may be in the processor, may be external to the processor, or distributed across multiple entities including the processor or processing circuitry. The computer-readable storage medium/memory described above may be embodied in a computer program product. By way of example, a computer program product may include a computer-readable medium in packaging material.
Alternatively, embodiments of the present application also provide a general-purpose processing system, such as that commonly referred to as a chip, including one or more microprocessors that provide processor functionality; and an external memory providing at least a portion of the storage medium, all connected together with other supporting circuitry through an external bus architecture. The memory stored instructions, when executed by the processor, cause the processor to perform some or all of the steps of the magnetic bead assay detection apparatus in the embodiments of fig. 4-5, such as steps 402-404 of fig. 4, steps 502-504 of fig. 5, and/or other processes for the techniques described herein.
The steps of a method or algorithm described in connection with the disclosure herein may be embodied in hardware or in software instructions executed by a processor. The software instructions may consist of corresponding software modules that may be stored in RAM memory, flash memory, ROM memory, EPROM memory, EEPROM memory, registers, a hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art. An exemplary storage medium is coupled to the processor such the processor can read information from, and write information to, the storage medium. Of course, the storage medium may also be integral to the processor. The processor and the storage medium may reside in an ASIC. Additionally, the ASIC may reside in user equipment. Of course, the processor and the storage medium may reside as discrete components in user equipment.
Specifically, referring to fig. 8, the magnetic bead assay detection device 800 of the present embodiment includes: an input module 801, a processing module 802 and an output module 803. The device 800 may be the magnetic bead assay device in the above-described method embodiments, or may be one or more chips in the magnetic bead assay device. The device 800 may be used to perform some or all of the functions of the magnetic bead assay detection device in the above-described method embodiments.
For example, the input module 801 may be used to perform the step 501 in the above method embodiments. For example, the input module 801 obtains a gravity component and a buoyancy component of the magnetic bead in the horizontal direction.
The processing module 802 may be configured to perform steps 502 to 504 in the above method embodiments. For example, the processing module 802 determines a driving parameter adjustment amount according to the gravity component and the buoyancy component; determining a driving parameter according to the driving parameter adjustment amount; and driving the magnetic beads according to the driving parameters, and detecting the reaction endpoint of the reaction sample.
The output module 803 may be used to output the reaction endpoint or output a test waveform during the test.
Optionally, the apparatus 800 further comprises a storage module coupled to the processing module, so that the processing module can execute computer-executable instructions stored in the storage module to implement the functions of the magnetic bead method detection apparatus in the above-described method embodiments. In one example, the memory module optionally included in the apparatus 800 may be a memory unit inside the chip, such as a register, a cache, or the like, and the memory module may also be a memory unit located outside the chip, such as a read-only memory (ROM) or another type of static memory device that can store static information and instructions, a Random Access Memory (RAM), or the like.
It should be understood that the flow executed between the modules of the magnetic bead method detection device in the corresponding embodiment of fig. 8 is similar to the flow executed by the magnetic bead method detection device in the corresponding embodiment of fig. 5, and details thereof are not repeated here.
Fig. 9 shows a schematic diagram of a possible structure of the magnetic bead assay detection device 900 in the above embodiment, and the device 900 may be configured as the magnetic bead assay detection device. The apparatus 900 may include: a processor 902, a computer-readable storage medium/memory 903, a transceiver 904, an input device 905, and an output device 908, and a bus 901. Wherein the processor, transceiver, computer readable storage medium, etc. are connected by a bus. The embodiments of the present application do not limit the specific connection medium between the above components.
In one example, the input device 905 acquires a gravity component and a buoyancy component of the magnetic bead in a horizontal direction;
the processor 902 determines a driving parameter adjustment amount according to the gravity component and the buoyancy component; determining a driving parameter according to the driving parameter adjustment amount; and driving the magnetic beads according to the driving parameters, and detecting the reaction endpoint of the reaction sample.
In one example, the processor 902 may include baseband circuitry, e.g., may generate drive parameters.
In yet another example, the processor 902 may run an operating system that controls functions between various devices and appliances. The transceiver 904 may include baseband circuitry and radio frequency circuitry.
The input device 905, the output device 908, and the processor 902 may implement the steps corresponding to any embodiment in fig. 5, which is not described herein again.
It is understood that fig. 9 only shows a simplified design of the magnetic bead assay detection device, and in practical applications, the magnetic bead assay detection device may include any number of transceivers, processors, memories, etc., and all magnetic bead assay detection devices that can implement the present application are within the scope of the present application.
The processor 902 involved in the apparatus 900 may be a general-purpose processor, such as a general-purpose Central Processing Unit (CPU), a Network Processor (NP), a microprocessor, etc., or an application-specific integrated circuit (ASIC), or one or more integrated circuits for controlling the execution of the program according to the present application. But also a Digital Signal Processor (DSP), a field-programmable gate array (FPGA) or other programmable logic device, discrete gate or transistor logic device, discrete hardware components. The controller/processor can also be a combination of computing functions, e.g., comprising one or more microprocessors, DSPs, and microprocessors, among others. Processors typically perform logical and arithmetic operations based on program instructions stored within memory.
The bus 901 may be a Peripheral Component Interconnect (PCI) bus, an Extended Industry Standard Architecture (EISA) bus, or the like. The bus may be divided into an address bus, a data bus, a control bus, etc. For ease of illustration, only one thick line is shown in FIG. 9, but this does not indicate only one bus or one type of bus.
The computer-readable storage medium/memory 903 referred to above may also hold an operating system and other application programs. In particular, the program may include program code including computer operating instructions. More specifically, the memory may be a read-only memory (ROM), other types of static storage devices that may store static information and instructions, a Random Access Memory (RAM), other types of dynamic storage devices that may store information and instructions, a disk memory, and so forth. The memory 903 may be a combination of the above memory types. And the computer-readable storage medium/memory described above may be in the processor, may be external to the processor, or distributed across multiple entities including the processor or processing circuitry. The computer-readable storage medium/memory described above may be embodied in a computer program product. By way of example, a computer program product may include a computer-readable medium in packaging material.
Alternatively, embodiments of the present application also provide a general-purpose processing system, such as that commonly referred to as a chip, including one or more microprocessors that provide processor functionality; and an external memory providing at least a portion of the storage medium, all connected together with other supporting circuitry through an external bus architecture. The memory stored instructions, when executed by the processor, cause the processor to perform some or all of the steps of the magnetic bead assay detection apparatus in the embodiment of fig. 5, such as steps 502-504 of fig. 5, and/or other processes for the techniques described herein.
The steps of a method or algorithm described in connection with the disclosure herein may be embodied in hardware or in software instructions executed by a processor. The software instructions may consist of corresponding software modules that may be stored in RAM memory, flash memory, ROM memory, EPROM memory, EEPROM memory, registers, a hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art. An exemplary storage medium is coupled to the processor such the processor can read information from, and write information to, the storage medium. Of course, the storage medium may also be integral to the processor. The processor and the storage medium may reside in an ASIC. Additionally, the ASIC may reside in user equipment. Of course, the processor and the storage medium may reside as discrete components in user equipment.
It is clear to those skilled in the art that, for convenience and brevity of description, the specific working processes of the above-described systems, apparatuses and units may refer to the corresponding processes in the foregoing method embodiments, and are not described herein again.
In the several embodiments provided in the present application, it should be understood that the disclosed system, apparatus and method may be implemented in other manners. For example, the above-described apparatus embodiments are merely illustrative, and for example, the division of the units is only one logical division, and other divisions may be realized in practice, for example, a plurality of units or components may be combined or integrated into another system, or some features may be omitted, or not executed. In addition, the shown or discussed mutual coupling or direct coupling or communication connection may be an indirect coupling or communication connection through some interfaces, devices or units, and may be in an electrical, mechanical or other form.
The units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of the embodiment.
In addition, functional units in the embodiments of the present application may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit. The integrated unit can be realized in a form of hardware, and can also be realized in a form of a software functional unit.
The integrated unit, if implemented in the form of a software functional unit and sold or used as a stand-alone product, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present application may be substantially implemented or contributed to by the prior art, or all or part of the technical solution may be embodied in a software product, which is stored in a storage medium and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the steps of the method according to the embodiments of the present application. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-only Memory (ROM), a Random Access Memory (RAM), a magnetic disk or an optical disk, and other various media capable of storing program codes.
The above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions in the embodiments of the present application.
Claims (32)
1. A detection method by a magnetic bead method is characterized by comprising the following steps:
obtaining a test item of a reaction sample;
determining a sample proportion according to the test item, wherein the sample proportion is used for representing the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample;
determining a driving parameter according to the sample ratio;
and driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
2. The method of claim 1, wherein determining drive parameters based on the sample ratios comprises:
and determining a target driving voltage according to the mapping relation between the sample ratio and the driving voltage, wherein the target driving voltage is used as the driving parameter.
3. The method of claim 2, wherein the sample ratio is mapped to the driving voltage by: vmag＝klg(Rsample)+b；
Wherein, the VmagFor the driving voltage, the RsampleFor the sample fraction, k and b are fitting coefficients.
4. The method of any one of claims 1 to 3, wherein said determining a sample fraction from said test items comprises:
obtaining the sample proportion from a reaction sample label corresponding to the test item, wherein the reaction sample label is used for indicating the composition of the reaction sample;
or the like, or, alternatively,
acquiring a mapping relation between the test item and the sample ratio;
and determining the sample proportion according to the mapping relation between the test item and the sample proportion.
5. The method of claim 1, further comprising:
acquiring a gravity component and a buoyancy component of the magnetic beads in the horizontal direction;
determining a driving parameter adjustment amount according to the gravity component and the buoyancy component;
and determining the driving parameters according to the driving parameter adjustment amount.
6. The method of claim 5, wherein determining a drive parameter adjustment based on the gravity component and the buoyancy component comprises:
determining a magnetic force adjustment amount according to the gravity component and the buoyancy component;
and determining the driving voltage adjustment amount according to the secondary positive correlation of the magnetic force adjustment amount and the driving voltage.
7. The method of claim 6, wherein determining a magnetic force adjustment from the gravitational component and the buoyancy component comprises:
determining the magnetic force adjustment amount by utilizing a first formula according to the gravity component and the buoyancy component;
the first formula is:
ΔFM＝±(G-FB)sinβ；
wherein, the Δ FMThe G is the gravity borne by the magnetic beads, and the F is the magnetic force adjustment amountBThe beta is an included angle between the magnetic force applied to the magnetic bead and the horizontal direction.
8. The method of claim 5, wherein determining a drive parameter adjustment from the gravity component and the buoyancy component comprises:
determining a magnetic force adjustment amount according to the gravity component and the buoyancy component, and determining the driving voltage adjustment amount according to the secondary positive correlation between the magnetic force adjustment amount and the driving voltage;
and determining a driving duty ratio adjustment amount according to the gravity component and the buoyancy component, wherein the driving voltage adjustment amount and the driving duty ratio adjustment amount are used as the driving parameter adjustment amount.
9. The method of claim 8, wherein determining a drive duty cycle adjustment from the gravitational component and the buoyancy component comprises:
determining a driving duty ratio adjustment amount by using a second formula according to the gravity component and the buoyancy component;
the second formula is:
wherein Δ t is the driving duty ratio adjustment amount, G is the gravity borne by the magnetic bead, and F is the weightBIs the buoyancy to which the magnetic beads are subjected, FMThe beta is an included angle between the magnetic force applied to the magnetic bead and the horizontal direction.
10. The method of any one of claims 5 to 9, wherein the obtaining the gravitational component and the buoyancy component of the magnetic beads comprises:
acquiring an included angle between the magnetic force borne by the magnetic bead and the horizontal direction according to the levelness sensor;
determining the gravity component and the buoyancy component of the magnetic beads in the horizontal direction according to the included angle;
or the like, or, alternatively,
acquiring an included angle between the magnetic force borne by the magnetic bead and the horizontal direction according to at least two position sensors;
and determining the gravity component and the buoyancy component of the magnetic beads in the horizontal direction according to the included angle.
11. A detection method by a magnetic bead method is characterized by comprising the following steps:
acquiring a gravity component and a buoyancy component of the magnetic beads in the horizontal direction;
determining a driving parameter adjustment amount according to the gravity component and the buoyancy component;
determining a driving parameter according to the driving parameter adjustment amount;
and driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample.
12. The method of claim 11, wherein determining a drive parameter adjustment based on the gravity component and the buoyancy component comprises:
determining a magnetic force adjustment amount according to the gravity component and the buoyancy component;
and determining the driving voltage adjustment amount according to the secondary positive correlation of the magnetic force adjustment amount and the driving voltage.
13. The method of claim 12, wherein determining a magnetic force adjustment from the gravitational component and the buoyancy component comprises:
determining the magnetic force adjustment amount by utilizing a first formula according to the gravity component and the buoyancy component;
the first formula is:
ΔFM＝±(G-FB)sinβ；
wherein, the Δ FMThe G is the gravity borne by the magnetic beads, and the F is the magnetic force adjustment amountBThe beta is an included angle between the magnetic force applied to the magnetic bead and the horizontal direction.
14. The method of claim 11, wherein determining a drive parameter adjustment based on the gravity component and the buoyancy component comprises:
determining a magnetic force adjustment amount according to the gravity component and the buoyancy component, and determining the driving voltage adjustment amount according to the secondary positive correlation between the magnetic force adjustment amount and the driving voltage;
and determining a driving duty ratio adjustment amount according to the gravity component and the buoyancy component, wherein the driving voltage adjustment amount and the driving duty ratio adjustment amount are used as the driving parameter adjustment amount.
15. The method of claim 14, wherein determining a drive duty cycle adjustment from the gravitational component and the buoyancy component comprises:
determining a driving duty ratio adjustment amount by using a second formula according to the gravity component and the buoyancy component;
the second formula is:
wherein Δ t is the driving duty ratio adjustment amount, G is the gravity borne by the magnetic bead, and F is the weightBIs the buoyancy to which the magnetic beads are subjected, FMThe beta is an included angle between the magnetic force applied to the magnetic bead and the horizontal direction.
16. The method of any one of claims 11 to 15, wherein the obtaining the gravitational component and the buoyancy component of the magnetic beads comprises:
acquiring an included angle between the magnetic force borne by the magnetic bead and the horizontal direction according to the levelness sensor;
determining the gravity component and the buoyancy component of the magnetic beads in the horizontal direction according to the included angle;
or the like, or, alternatively,
acquiring an included angle between the magnetic force borne by the magnetic bead and the horizontal direction according to at least two position sensors;
and determining the gravity component and the buoyancy component of the magnetic beads in the horizontal direction according to the included angle.
17. A detection device by a magnetic bead method is characterized by comprising: an input device, a processor, an output device;
the input equipment is used for acquiring a test item of a reaction sample;
the processor is used for determining a sample proportion according to the test item, and the sample proportion is used for representing the percentage of fibrinogen in the reaction sample to the total volume of the reaction sample; determining a driving parameter according to the sample ratio; driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample;
the output device is used for outputting the reaction end point.
18. The apparatus according to claim 17, wherein the processor is specifically configured to determine a target driving voltage according to a mapping relationship between the sample ratio and the driving voltage, and the target driving voltage is used as the driving parameter.
19. The apparatus of claim 18, wherein the sample ratio is mapped to the driving voltage by: vmag＝klg(Rsample)+b；
Wherein, the VmagFor the driving voltage, the RsampleFor the sample fraction, k and b are fitting coefficients.
20. The apparatus according to any one of claims 17 to 19, wherein the processor is specifically configured to obtain the sample fraction from a reaction sample tag corresponding to the test item, the reaction sample tag being indicative of a composition of the reaction sample;
or the like, or, alternatively,
acquiring a mapping relation between the test item and the sample ratio;
and determining the sample proportion according to the mapping relation between the test item and the sample proportion.
21. The apparatus of claim 17, wherein the input device is further configured to obtain a gravitational component and a buoyancy component of the magnetic bead;
the processor is further used for determining a driving parameter adjustment amount according to the gravity component and the buoyancy component; and determining the driving parameters according to the driving parameter adjustment amount.
22. The apparatus of claim 21, wherein the processor is configured to determine a magnetic force adjustment based on the gravitational component and the buoyancy component;
and determining the driving voltage adjustment amount according to the secondary positive correlation of the magnetic force adjustment amount and the driving voltage.
23. The apparatus of claim 22, wherein the processor is configured to determine the magnetic force adjustment using a first formula based on the gravitational component and the buoyancy component;
the first formula is:
ΔFM＝±(G-FB)sinβ；
wherein, the Δ FMThe G is the gravity borne by the magnetic beads, and the F is the magnetic force adjustment amountBThe beta is an included angle between the magnetic force applied to the magnetic bead and the horizontal direction.
24. The apparatus according to claim 21, wherein the processor is specifically configured to determine a magnetic force adjustment amount according to the gravity component and the buoyancy component, and determine the driving voltage adjustment amount according to a quadratic positive correlation between the magnetic force adjustment amount and the driving voltage;
and determining a driving duty ratio adjustment amount according to the gravity component and the buoyancy component, wherein the driving voltage adjustment amount and the driving duty ratio adjustment amount are used as the driving parameter adjustment amount.
25. The apparatus of claim 24, wherein the processor is specifically configured to determine a drive duty cycle adjustment based on the gravity component and the buoyancy component using a second formula;
the second formula is:
wherein Δ t is the driving duty ratio adjustment amount, G is the gravity borne by the magnetic bead, and F is the weightBIs the buoyancy to which the magnetic beads are subjected, FMThe beta is an included angle between the magnetic force applied to the magnetic bead and the horizontal direction.
26. The apparatus according to any one of claims 21 to 25, wherein the input device is specifically configured to obtain an included angle between a magnetic force applied to the magnetic bead and a horizontal direction according to a levelness sensor;
determining the gravity component and the buoyancy component of the magnetic beads in the horizontal direction according to the included angle;
or the like, or, alternatively,
acquiring an included angle between the magnetic force borne by the magnetic bead and the horizontal direction according to at least two position sensors;
and determining the gravity component and the buoyancy component of the magnetic beads in the horizontal direction according to the included angle.
27. A detection device by a magnetic bead method is characterized by comprising: an input device, a processor, and an output device;
the input device is used for acquiring a gravity component and a buoyancy component of the magnetic beads;
the processor is used for determining a driving parameter adjustment amount according to the gravity component and the buoyancy component;
determining a driving parameter according to the driving parameter adjustment amount;
driving the magnetic beads according to the driving parameters, and detecting the reaction end point of the reaction sample;
the output device is used for outputting the reaction end point.
28. The apparatus of claim 27, wherein the processor is configured to determine a magnetic force adjustment based on the gravitational component and the buoyancy component; and determining the driving voltage adjustment amount according to the secondary positive correlation of the magnetic force adjustment amount and the driving voltage.
29. The apparatus according to claim 28, wherein the processor is configured to determine the magnetic force adjustment using a first formula based on the gravitational component and the buoyancy component; the first formula is:
ΔFM＝±(G-FB)sinβ；
wherein, the Δ FMThe G is the gravity borne by the magnetic beads, and the F is the magnetic force adjustment amountBThe beta is an included angle between the magnetic force applied to the magnetic bead and the horizontal direction.
30. The apparatus according to claim 27, wherein the processor is specifically configured to determine a magnetic force adjustment amount according to the gravity component and the buoyancy component, and determine the driving voltage adjustment amount according to a quadratic positive correlation between the magnetic force adjustment amount and the driving voltage;
and determining a driving duty ratio adjustment amount according to the gravity component and the buoyancy component, wherein the driving voltage adjustment amount and the driving duty ratio adjustment amount are used as the driving parameter adjustment amount.
31. The apparatus of claim 30, wherein the processor is specifically configured to determine a drive duty cycle adjustment based on the gravity component and the buoyancy component using a second formula;
the second formula is:
wherein Δ t is the driving duty ratio adjustment amount, G is the gravity borne by the magnetic bead, andFBis the buoyancy to which the magnetic beads are subjected, FMThe beta is an included angle between the magnetic force applied to the magnetic bead and the horizontal direction.
32. The apparatus according to any one of claims 27 to 31, wherein the input device is specifically configured to obtain an included angle between a magnetic force applied to the magnetic bead and a horizontal direction according to a levelness sensor; determining the gravity component and the buoyancy component of the magnetic beads in the horizontal direction according to the included angle;
or the like, or, alternatively,
acquiring an included angle between the magnetic force borne by the magnetic bead and the horizontal direction according to at least two position sensors;
and determining the gravity component and the buoyancy component of the magnetic beads in the horizontal direction according to the included angle.
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| CN112433058B (en) * | 2020-11-20 | 2023-09-15 | 迈克医疗电子有限公司 | Method, device, terminal equipment and medium for detecting and controlling magnetic beads |
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