CN110161007A - A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence - Google Patents
A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence Download PDFInfo
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- CN110161007A CN110161007A CN201910476955.7A CN201910476955A CN110161007A CN 110161007 A CN110161007 A CN 110161007A CN 201910476955 A CN201910476955 A CN 201910476955A CN 110161007 A CN110161007 A CN 110161007A
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- 238000001514 detection method Methods 0.000 title claims abstract description 156
- 230000001360 synchronised effect Effects 0.000 title claims abstract description 20
- 230000003287 optical effect Effects 0.000 title claims abstract description 18
- 230000005284 excitation Effects 0.000 claims abstract description 30
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 20
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000013307 optical fiber Substances 0.000 claims abstract description 9
- 239000000835 fiber Substances 0.000 claims abstract description 8
- 229910000831 Steel Inorganic materials 0.000 claims description 14
- 239000010959 steel Substances 0.000 claims description 14
- 230000006698 induction Effects 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229910052736 halogen Inorganic materials 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence, including detection pedestal and the detection module for being placed with reagent, it is provided with excitation circle in the detection pedestal and receives circle, the excitation circle is connect by excitation fiber with detection module, and the reception circle is connect by reception optical fiber with detection module；Stepper motor is installed in the detection pedestal, the detection turntable that rotation is driven by it is installed on output shaft of stepper motor, the one-to-one detection components of the multiple groups rotated with it and light source assembly are installed on the detection turntable, the light source assembly is located at the underface of excitation circle, and the detection components are located at the underface for receiving circle.Detection turntable of the invention, which rotates a circle, can be realized multiple Channel Synchronous detection of different reagents, greatly improve detection efficiency.
Description
Technical field
The invention belongs to biology and technical field of medical detection, are related to a kind of light for the synchronous detection of multi-wavelength fluorescence
Learn detection device.
Background technique
Real-Time Fluorescent Quantitative PCR Technique is able to achieve the quantitative analysis to DNA profiling, grinds to molecular biology research and medicine
Study carefully etc. and to be of great significance.The detection of major part quantitative PCR apparatus uses substantially at present takes pictures or is detected by optical fiber to reagent,
Wherein using shooting style since light source generally uses halogen lamp, halogen lamp, which is generally used for a long time to decay, leads to measurement result not
Standard, and to have edge effect to also result in measurement result inaccurate for photo of taking pictures, and another using optical fiber and cooperate PMT light
Electric multiplier tube is detected, and the mechanism is since PMT photomultiplier tube is more expensive and volume is bigger, when detecting to reagent only
Being able to cooperate linear guide or screw rod, scanning mode detects one by one to reagent progress, if reagent is multichannel reagent, detection
Need to wait an Air conduct measurement is complete could switch next channel and detected when the channel Shi Genghuan, such detection rates compare
Slowly, it is not suitable for if reagent requires this detection mode to detection rates.
Summary of the invention
The present invention is directed to the fact that current, provides a kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence,
It will first need detection reagent to guide in a detection plate by optical fiber, and in use PD and with slip ring mode, it carries out while examining
It surveys, greatly improves detection efficiency.
The technical solution adopted by the present invention is that:
A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence, including detection pedestal and the inspection for being placed with reagent
Survey module, it is characterised in that: be provided with excitation circle in the detection pedestal and receive circle, excitation circle by excitation fiber and
Detection module connection, the reception circle are connect by reception optical fiber with detection module；Stepping electricity is installed in the detection pedestal
Machine is equipped with the detection turntable that rotation is driven by it on the output shaft of the stepper motor, be equipped on the detection turntable with
Its one-to-one detection components of multiple groups rotated and light source assembly, the light source assembly is located at the underface of excitation circle, described
Detection components are located at the underface for receiving circle.Detection turntable of the invention, which rotates a circle, can be realized multiple channels of different reagents
Synchronous detection, greatly improves detection efficiency.
Further, the light source control line of the light source assembly and the signal wire of the detection components pass through on detection turntable
Slip ring is connect with external control panel, line can be intertwined to avoid when rotating.
Further, 6 groups of detection components and light source assembly, detection components described in every group are uniformly installed on the detection turntable
It is staggered 30 ° and is arranged with light source assembly.
Further, the inside for receiving circle is arranged in the excitation circle, and the excitation circle is respectively provided with reception circle
There are 96 holes.
Further, the test tube hole of 96 placement reagents is provided in the detection module.
Further, the detection components include detection set, are sequentially installed with the first convex lens in the detection set, receive filter
Mating plate, insulated column, PD detection plate, the end cover that the detection set is provided with PD detection plate are covered equipped with PD, first convex lens
Towards detection circle setting.
Further, the light source assembly includes light source set, is sequentially installed with the second convex lens, convex lens in the light source set
Seat, exciter filter, plastic column, LED lamp panel, the LED lamp panel be set to light source cover end, second convex lens to swash
Headband setting.
Further, origin detection Hall Plate is installed in the detection pedestal, origin magnetic is installed on the detection turntable
Steel seat is equipped with the magnet steel of induction origin detection Hall Plate operation in the origin magnet steel seat.Stepper motor operation of the invention
One week is to incude origin Hall Plate by the magnet steel in origin magnet steel seat to run to origin position to realize, guarantees the reliable of detection
Property and accuracy.
The present invention have the advantages that it is following prominent with the utility model has the advantages that being provided with the detection components and light source in the different channels of multiple groups
Component, and realize that multiple Channel Synchronous of different reagents detect by one circle of rotation, greatly improve detection efficiency.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention.
Fig. 2 is the schematic top plan view of part-structure of the invention.
Fig. 3 is that part-structure of the invention looks up stereoscopic schematic diagram.
Fig. 4 is the schematic top plan view after present invention removal detection pedestal.
Fig. 5 is the configuration schematic diagram of detection components of the invention.
Fig. 6 is the configuration schematic diagram of light source assembly of the invention.
In figure: 1- stepper motor, 2- detection pedestal, 3- detection components, 4- light source assembly, 5- slip ring, 6- detect turntable, 7-
Excitation fiber, 8- reception optical fiber, 9- origin magnet steel seat, 10- origin detection Hall Plate, 11- detection set, the first convex lens of 12-,
13- receives optical filter, 14- insulated column, 15-PD detection plate, 16-PD lid, 17- light source set, 18- convex lens microscope base, 19- excitation optical filtering
Piece, 20- plastic column, 21-LED lamp plate, 22- module, the second convex lens of 23-, 24- receive circle, 25- excitation circle
Specific embodiment
Next combined with specific embodiments below invention is further explained, but does not limit the invention to these tools
Body embodiment.One skilled in the art would recognize that present invention encompasses may include in Claims scope
All alternatives, improvement project and equivalent scheme.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", " clockwise ", " inverse
The orientation or positional relationship of the instructions such as hour hands " is to be based on the orientation or positional relationship shown in the drawings, and is merely for convenience of description originally
Invention and simplified description, rather than the device or element of indication or suggestion meaning must have a particular orientation, with specific side
Position construction and operation, therefore be not considered as limiting the invention.In addition, term " first ", " second " are only used for description mesh
, it is not understood to indicate or imply relative importance or implicitly indicates the quantity of indicated technical characteristic.It limits as a result,
There is the feature of " first ", " second " to can explicitly or implicitly include one or more of the features surely.Of the invention
In description, unless otherwise indicated, the meaning of " plurality " is two or more, unless otherwise restricted clearly.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " fixation " etc.
Term shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can be machine
Tool connection, is also possible to be electrically connected；It can be directly connected, two members can also be can be indirectly connected through an intermediary
Connection inside part.For the ordinary skill in the art, above-mentioned term can be understood in this hair as the case may be
Concrete meaning in bright.
In the present invention unless specifically defined or limited otherwise, fisrt feature second feature "upper" or "lower"
It may include that the first and second features directly contact, also may include that the first and second features are not direct contacts but pass through it
Between other characterisation contact.Moreover, fisrt feature includes the first spy above the second feature " above ", " above " and " above "
Sign is right above second feature and oblique upper, or is merely representative of first feature horizontal height higher than second feature.Fisrt feature exists
Second feature " under ", " lower section " and " following " include that fisrt feature is directly below and diagonally below the second feature, or is merely representative of
First feature horizontal height is less than second feature.
Referring to Fig.1-4, a kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence is present embodiments provided, including
Detection pedestal 2 and the detection module 22 for being placed with reagent are provided with excitation circle 25 in the detection pedestal 2 and receive circle 24, institute
It states excitation circle 25 and the inside for receiving circle 24 is set, the excitation circle 25 is provided with 96 holes with reception circle 24,
Correspondingly, be provided with the test tube holes of 96 placement reagents in the detection module 22, excitation circle 25 by excitation fiber 8 and
Detection module 22 connects, and the reception circle 24 is connect by reception optical fiber 7 with detection module 22.
2 are equipped with stepper motor 1 in detection pedestal described in the present embodiment, are equipped on the output shaft of the stepper motor 1
The detection turntable 6 of rotation is driven by it, and six groups rotated with it are uniformly installed on the detection turntable 6 and are detected correspondingly
Component 3 and light source assembly 4, detection components 3 are equipped with different reception optical filters, and light source assembly 4 is equipped with different excitation filters
Mating plate, to form different sense channel and excitation channel, detection components 3 and light source assembly 4 described in every group are staggered 30 ° and set
It sets；The underface that the light source assembly 4 is located at excitation circle 25 is corresponding with corresponding optic fibre hole, and the detection components 3, which are located at, receives circle
24 underface is corresponding with corresponding optic fibre hole；The light source control line of the light source assembly 4 and the signal wire of the detection components 3 are logical
The slip ring 5 crossed on detection turntable 6 is connect with external control panel carries out data processing by control panel, can to avoid when rotating by line
It is intertwined.Origin detection Hall Plate 10 is installed in the detection pedestal 2, origin magnetic is installed on the detection turntable 6
Steel seat 9 is equipped with the magnet steel that induction origin detection Hall Plate 10 is run in the origin magnet steel seat 9.Stepper motor of the invention
Running one week is to incude origin Hall Plate by the magnet steel in origin magnet steel seat to run to origin position to realize, guarantees detection
Reliability and accuracy.Detection turntable of the invention, which rotates a circle, can be realized multiple Channel Synchronous detection of different reagents, greatly
It is big to improve detection efficiency.
Detection components 3 described in the present embodiment include detection set 11, are sequentially installed with the first convex lens in the detection set 11
12, optical filter 13, insulated column 14, PD detection plate 15 are received, the end cover that the detection set 11 is provided with PD detection plate 15 is equipped with
PD lid 16, first convex lens 12 is towards 24 setting of detection circle, referring to Fig. 5.The light source assembly 4 includes light source set 17, institute
It states in light source set 17 and is sequentially installed with the second convex lens 23, convex lens microscope base 18, exciter filter 19, plastic column 20, LED lamp panel
21, the LED lamp panel 21 is set to light source and covers 17 ends, and second convex lens 23 is towards 25 setting of excitation circle, referring to Fig. 6.
The detection components 3 in different channels of the invention and the light source assembly 4 in different channels be fixed on detection turntable 6 on and
The signal wire of control line and detection components 4 that light source group is 3 is welded on transit line plate, and by being fixed on detection turntable 6
On slip ring 5 on conducting wire be drawn out on control panel and carry out data processing, the conducting wire sparetime transit line plate connection on slip ring 5,
More excitation fibers and reception optical fiber one end are fixed in detection pedestal 2, and the other end is fixed on detection module 22, detection module
Reagent is put on 22, stepper motor 1 is fixed in detection pedestal 2, and detection turntable 6 is fixed by screws in the defeated of stepper motor 1
On shaft, one circle of the rotation of stepper motor 1,6 groups of detection components 3 and light source assembly 4 detect simultaneously in actual moving process, operation one
Circle just completes the detection to 96 holes, can be realized and realizes multiple Channel Synchronous detections to different reagents, substantially increases detection
Efficiency.
Claims (8)
1. a kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence, including detection pedestal and the detection for being placed with reagent
Module, it is characterised in that: be provided with excitation circle in the detection pedestal and receive circle, the excitation circle passes through excitation fiber and inspection
Module connection is surveyed, the reception circle is connect by reception optical fiber with detection module；Stepper motor is installed in the detection pedestal,
The detection turntable for driving rotation by it is installed on output shaft of stepper motor, is equipped on the detection turntable with its turn
The one-to-one detection components of dynamic multiple groups and light source assembly, the light source assembly are located at the underface of excitation circle, the detection
Component is located at the underface for receiving circle.
2. a kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence according to claim 1, it is characterised in that:
The control that the light source control line of the light source assembly and the signal wire of the detection components pass through slip ring and outside on detection turntable
Making sheet connection.
3. a kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence according to claim 1, it is characterised in that:
6 groups of detection components and light source assembly are uniformly installed, detection components described in every group and light source assembly are staggered on the detection turntable
30 ° of settings.
4. a kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence according to claim 1, it is characterised in that:
The inside for receiving circle is arranged in the excitation circle, and the excitation circle is provided with 96 holes with reception circle.
5. a kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence according to claim 4, it is characterised in that:
The test tube hole of 96 placement reagents is provided in the detection module.
6. the optical detection apparatus detected is synchronized for multi-wavelength fluorescence to a kind of described in 5 any one according to claim 1,
It is characterized by: the detection components include detection set, it is sequentially installed with the first convex lens in the detection set, receives and filters
Piece, insulated column, PD detection plate, the end cover that the detection set is provided with PD detection plate are covered equipped with PD, first convex lens
To detection circle setting.
7. the optical detection apparatus detected is synchronized for multi-wavelength fluorescence to a kind of described in 5 any one according to claim 1,
It is characterized by: the light source assembly includes light source set, be sequentially installed in the light source set the second convex lens, convex lens microscope base,
Exciter filter, plastic column, LED lamp panel, the LED lamp panel are set to light source and cover end, and second convex lens are to excitation
Circle setting.
8. the optical detection apparatus detected is synchronized for multi-wavelength fluorescence to a kind of described in 5 any one according to claim 1,
It is characterized by: being equipped with origin detection Hall Plate in the detection pedestal, origin magnet steel seat is installed on the detection turntable,
The magnet steel of induction origin detection Hall Plate operation is installed in the origin magnet steel seat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910476955.7A CN110161007A (en) | 2019-06-03 | 2019-06-03 | A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910476955.7A CN110161007A (en) | 2019-06-03 | 2019-06-03 | A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence |
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| CN110161007A true CN110161007A (en) | 2019-08-23 |
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| CN201910476955.7A Pending CN110161007A (en) | 2019-06-03 | 2019-06-03 | A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109085148A (en) * | 2018-10-11 | 2018-12-25 | 滨江华康（北京）生物科技有限公司 | A kind of multichannel fluorescence detection optical system |
| CN111474151A (en) * | 2020-04-18 | 2020-07-31 | 杭州准芯生物技术有限公司 | Liquid detection method |
| WO2022156824A1 (en) * | 2021-01-23 | 2022-07-28 | 利多(香港)有限公司 | Analyzer and method for reading detection result of test apparatus |
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| CN210514085U (en) * | 2019-06-03 | 2020-05-12 | 杭州安誉科技有限公司 | Optical detection device for multi-wavelength fluorescence synchronous detection |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109085148A (en) * | 2018-10-11 | 2018-12-25 | 滨江华康（北京）生物科技有限公司 | A kind of multichannel fluorescence detection optical system |
| CN111474151A (en) * | 2020-04-18 | 2020-07-31 | 杭州准芯生物技术有限公司 | Liquid detection method |
| WO2022156824A1 (en) * | 2021-01-23 | 2022-07-28 | 利多(香港)有限公司 | Analyzer and method for reading detection result of test apparatus |
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