CN108642158A - A kind of PCR real-time fluorescence detection systems of multichannel point detection - Google Patents
A kind of PCR real-time fluorescence detection systems of multichannel point detection Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 26
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- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
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- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
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- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
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- 238000003752 polymerase chain reaction Methods 0.000 description 1
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- C12Q1/686—Polymerase chain reaction [PCR]
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- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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Abstract
The invention discloses a kind of PCR real-time fluorescence detection systems of multichannel point detection, including thermocirculator, sample changeover module includes that motor fixes disk, stepper motor, and motor turntable, stepper motor is fixed motor and is fixed on disk, motor turntable is sequentially connected with stepper motor, motor fixes disk and is equipped with the exciting light optical fiber fixed hole position consistent with example reaction pool count and transmitting light fiber-optic fixing-hole position, exciting light optical fiber fixed hole position is corresponding with transmitting light fiber-optic fixing-hole position and fixes disk circumferentially along motor respectively, each example reaction pond side wall is equipped with excitation optical delivery fiber and transmitting optical delivery fiber, excitation optical delivery fiber and transmitting optical delivery fiber are connected to mutual corresponding exciting light optical fiber fixed hole position and transmitting light fiber-optic fixing-hole position；Multiple excitation light source components are arranged on motor turntable；Multiple fluoroscopic examination components are arranged on motor turntable；And control device.The PCR real-time fluorescence detection systems of multichannel point detection provided by the invention, compact-sized, accuracy of detection is high, reproducible.
Description
Technical field
The present invention relates to technical field of biological, more particularly to a kind of PCR real-time fluorescences detection of multichannel point detection
System.
Background technology
PCR (polymerase chain reaction, PCR) is a kind of specific for amplifying amplification
The Protocols in Molecular Biology of DNA fragmentation, it is considered as the special DNA cloning of in vitro, by high-temperature denatured, process annealing and
In several steps reaction one temperature cycles period of composition such as thermophilic extension, so that purpose is expanded rapidly, there is high specificity, sensitivity
The features such as high, easy to operate, time saving is a significant innovation of gene amplification.Round pcr can be special by the target of denier
Ground expands up to a million times, to greatly improve analysis and the detectability to DNA molecular.Extinct plants and animal either in fossil is gone through
The remains of history personage, hair, skin or the blood that still assailant is left in murder case decades ago, as long as can isolate micro
DNA, can be amplified with round pcr, be compared.This is also the place of the power of " micro evidence ".
Fluorescent quantitative PCR technique is that corresponding fluorescent dye or fluorescence labeling probe are added on the basis of Standard PCR,
Changed by fluorescence signal in PCR reaction process, entire PCR processes are measured in real time, surveyed with fluorescent chemical each
The method of product total amount after PCR (PCR) cycle, by internal reference or outer ginseng method to the specific DNA in sample to be tested
The method that sequence carries out quantitative analysis.
In general, fluorescence quantitative PCR instrument is the instrument of real-time detection reaction, and there are two function modules to ensure PCR reactions
The thermal cycler and fluorescence real-time detecting system of progress.This instrument is mainly examined by gene magnification thermal cycle component, particle fluorescence
Photometry system, microcircuit control system, computer and application software composition.Wherein gene magnification thermal cycle component operation principle
It is essentially identical with traditional gene-amplificative instrament, the working methods such as air heating, compressor cooling, semiconductor heating refrigeration can be used.
Fluorescence detecting system is mainly formed by by fluorescence excitation component, optical signal carrying components, fluoroscopic examination component, control system.
Motor is used to drive the fluoroscopic examination fiber spinning in connection example reaction pond in the prior art, to realize to difference
The detection of sample, this instrument it is complicated, multiple fluoroscopic examination optical fiber are easy to happen confusion.
Invention content
It is compact-sized it is an object of the present invention to provide a kind of PCR real-time fluorescence detection systems of multichannel point detection, detection essence
Degree is high.
Based on the above issues, technical solution provided by the invention is：
A kind of PCR real-time fluorescence detection systems of multichannel point detection, including thermocirculator, on the thermocirculator
Equipped with several example reaction ponds；
Further include：
Sample changeover module, including motor fix disk, stepper motor and motor turntable, the stepper motor and are fixed on institute
It states motor fixation disk one end and output shaft extends to the motor and fixes the disk other end, the motor turntable and the stepper motor
It is sequentially connected and is rotated under motor drive, the motor is fixed disk and swashed equipped with consistent with the example reaction pool count
Emitting optical fiber fixed hole position and transmitting light fiber-optic fixing-hole position, the exciting light optical fiber fixed hole position and transmitting light fiber-optic fixing-hole
Position is corresponding and fixes disk circumferentially along the motor respectively, each example reaction pond side wall is equipped with excitation optical transport
Optical fiber and transmitting optical delivery fiber, the excitation optical delivery fiber and transmitting optical delivery fiber are connected to mutual corresponding described sharp
Emitting optical fiber fixed hole position and transmitting light fiber-optic fixing-hole position；
Multiple excitation light source components are arranged on the motor turntable, after the motor turntable rotates, the excitation
Light source assembly is corresponding with the exciting light optical fiber fixed hole position to transmit exciting light into example reaction pond；
Multiple fluoroscopic examination components are arranged on the motor turntable, after the motor turntable rotates, the fluorescence
Detection components and the transmitting light fiber-optic fixing-hole position are corresponding to acquire the transmitting light in example reaction pond；
And control device, the thermocirculator, motor, laser light source module, fluoroscopic examination component and the control fill
Set electrical connection.
In some embodiments wherein, the excitation light source component includes the first lens barrel, is arranged in first mirror
Fluorescence excitation light source in cylinder and the first collimation lens set gradually along light direction, the first optical filter and first focus saturating
Mirror, first lens barrel one end are equipped with light hole, and the motor turntable is equipped with and the matched laser light source aperture of the light hole.
In some embodiments wherein, first lens barrel includes the first lens barrel ontology and the first chassis, and described the
One lens barrel ontology includes the first cylindrical body portion and the first discshaped body portion of connection, and the end in first cylindrical body portion is set
The light hole is set, the fluorescence excitation light source, the first collimation lens, the first optical filter, the first condenser lens are arranged described
In first lens barrel ontology, first chassis is fixed in first discshaped body portion.
In some embodiments wherein, the fluorescence excitation light source is LED light source.
In some embodiments wherein, the fluoroscopic examination component includes the second lens barrel, is arranged in second mirror
In cylinder and edge enters the second collimation lens, the second optical filter, photo-electric conversion element that light direction is set gradually, second lens barrel
One end is equipped with incidence hole, and the motor turntable is equipped with enters hole with the matched transmitting light of the incidence hole.
In some embodiments wherein, second lens barrel includes the second lens barrel ontology and the second chassis, and described the
Two lens barrel ontologies include the second cylindrical body portion and the second discshaped body portion of connection, and the end in second cylindrical body portion is set
The incidence hole is set, the photo-electric conversion element, the second collimation lens, the second optical filter are arranged in the second lens barrel ontology
Interior, second chassis is fixed in second discshaped body portion.
In some embodiments wherein, the photo-electric conversion element is photodiode, photomultiplier or photoelectricity
Avalanche diode.
In some embodiments wherein, the control device includes fluorescence control circuit board and motor driver, institute
It states fluorescence control circuit board and the stepper motor work, the thermocirculator, laser light is controlled by the motor driver
Source component, fluoroscopic examination component are respectively connected to the fluorescence control circuit board.
In some embodiments wherein, the motor fixes disk and is equipped with alignment sensor, for the motor
Positioning when reset, the alignment sensor are connect with the control device signal.
In some embodiments wherein, the motor fixes disk and is equipped with optical fiber fixed part, the optical fiber fixed part
In the form of annular discs, the excitation optical delivery fiber, transmitting optical delivery fiber extend to the excitation light through the optical fiber fixed part
Fine fixed hole position, transmitting light fiber-optic fixing-hole position.
Compared with prior art, it is an advantage of the invention that：
1, technical solution using the present invention drives excitation light source component and fluoroscopic examination component to rotate by motor, if
It is fixed to set excitation optical delivery fiber and transmitting optical delivery fiber on the side wall of example reaction pond, by by exciting light
Source component and fluoroscopic examination component rotate to the hole position of Transmission Fibers corresponding with example reaction pond the acquisition and inspection for completing fluorescence
It surveys, avoids in the prior art due to the problem of Transmission Fibers, which rotate, leads to fluoroscopic examination poor repeatability；
2, multiple excitation light source components and fluoroscopic examination group is arranged in technical solution using the present invention on motor turntable
Part can realize multi-channel detection by the way that different wavelength is arranged, compared with the prior art in realized by switching optical filter
The structure of multi-channel detection saves big quantity space, the miniaturization convenient for system and portability.
Description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, required use in being described below to embodiment
Attached drawing be briefly described, drawings in the following description are only some embodiments of the invention, for the common skill in this field
For art personnel, without creative efforts, other drawings may also be obtained based on these drawings.
Fig. 1 is a kind of structural schematic diagram of the PCR real-time fluorescence detection system embodiments of multichannel point detection of the present invention；
Fig. 2 is the structural schematic diagram of sample changeover module in the embodiment of the present invention；
Fig. 3 is the structural schematic diagram of excitation light source component in the embodiment of the present invention；
Fig. 4 is the structural schematic diagram of fluoroscopic examination component in the embodiment of the present invention
Wherein：
1, thermocirculator；1-1, example reaction pond；
2, sample changeover module；2-1, motor fix disk；2-2, stepper motor；2-3, motor turntable；2-4, optical fiber are fixed
Portion；
3, excitation light source component；3-1, the first lens barrel ontology；3-1a, the first cylindrical body portion；3-1b, the first discshaped body
Portion；3-1c, light hole；3-2, the first chassis；3-3, fluorescence excitation light source；3-4, the first collimation lens；3-5, the first optical filter；
3-6, the first condenser lens；
4, fluoroscopic examination component；4-1, the second lens barrel ontology；4-1a, the second cylindrical body portion；4-1b, the second discshaped body
Portion；4-1c, incidence hole；4-2, the second chassis；4-3, the second collimation lens；4-4, the second optical filter；4-5, photo-electric conversion element；
5, control device；5-1, fluorescence control circuit board；5-2, motor driver；
6, optical delivery fiber is excited；
7, transmitting optical delivery fiber；
8, alignment sensor.
Specific implementation mode
Said program is described further below in conjunction with specific embodiment.It should be understood that these embodiments are for illustrating
The present invention and be not limited to limit the scope of the invention.The implementation condition used in embodiment can be done according to the condition of specific producer
Further adjustment, the implementation condition being not specified is usually the condition in routine experiment.
It is the structural schematic diagram of the embodiment of the present invention referring to Fig. 1-4, it is glimmering in real time provides a kind of PCR of multichannel point detection
Optical detection system, including thermocirculator 1, thermocirculator 1 are equipped with several example reaction ponds 1-1, and thermocirculator 1 is
The prior art, the present invention repeat no more.
Further include sample changeover module 2, multiple laser light source modules 3, multiple fluoroscopic examination components 4 and control device 5, heat
Circulator 1, sample changeover module 2, laser light source module 3, fluoroscopic examination component 4 are electrically connected with control device 5.Control device
5 include fluorescence control circuit board 5-1 and motor driver 5-2, and fluorescence control circuit board 5-1 is controlled by motor driver 5-2
Stepper motor 2-2 work, thermocirculator 1, laser light source module 3, fluoroscopic examination component 4 are respectively connected to fluorescence control circuit
Plate 5-1.
Sample changeover module 2 includes motor fixation disk 2-1, stepper motor 2-2 and motor turntable 2-3, stepper motor 2-2 solid
It is scheduled on motor fixation one end disk 2-1 and output shaft extends to motor and fixes the disk 2-1 other ends, motor turntable 2-3 and stepper motor
2-2 is sequentially connected and is rotated in the case where stepper motor 2-2 drives, and motor fixes disk 2-1 equipped with consistent with example reaction pond 1-1 numbers
Exciting light optical fiber fixed hole position and transmitting light fiber-optic fixing-hole position, exciting light optical fiber fixed hole position and transmitting light fiber-optic fixing-hole
Position is corresponding and fixes disk 2-1 circumferentially along motor respectively, each example reaction pond 1-1 side wall is equipped with excitation optical transport light
Fibre 6 and transmitting optical delivery fiber 7 excite optical delivery fiber 6 and transmitting optical delivery fiber 7 to be connected to mutual corresponding excitation light
Fine fixed hole position and transmitting light fiber-optic fixing-hole position.
Multiple settings of excitation light source component 3 are arranged circumferentially on motor turntable 2-3, and along motor turntable 2-3, work as electricity
After the 2-3 rotations of machine turntable, excitation light source component 3 is corresponding with exciting light optical fiber fixed hole position to make laser light source and excitation optical transport light
6 coupling of fibre into example reaction pond 1-1 to transmit exciting light.
Multiple settings of fluoroscopic examination component 4 are arranged circumferentially on motor turntable 2-3, and along motor turntable 2-3, work as electricity
After the 2-3 rotations of machine turntable, fluoroscopic examination group 4 is corresponding with transmitting light fiber-optic fixing-hole position to make fluoroscopic examination component 4 and transmitting light
Transmission Fibers 7 are coupled to acquire the transmitting light in the 1-1 of example reaction pond, to complete to detect.
Wherein, excitation light source component 3 includes the first lens barrel, the fluorescence excitation light source 3-3 being arranged in the first lens barrel and edge
The first collimation lens 3-4, the first optical filter 3-5 and the first condenser lens 3-6 that light direction is set gradually, in the first lens barrel one
End is equipped with light hole 3-1c, is equipped with and the matched laser light source aperture of light hole 3-1c on motor turntable 2-3.Preferably, fluorescence
It is accurate spike filter that excitation light source 3-3, which uses LED light source, the first optical filter 3-5,.The light that LED light source is sent out is through the first standard
Emergent light is directional light after straight lens 3-4, and directional light reaches required wave-length coverage after the first optical filter 3-5, then through the
One condenser lens 3-6 is coupled to excitation optical delivery fiber 6, eventually arrives at example reaction pond 1-1, completes excitation process.
In this example, the first lens barrel includes the first lens barrel ontology 3-1 and the first chassis 3-2, and the first lens barrel ontology 3-1 includes connecting
The the first cylindrical body portion 3-1a and the second discshaped body portion 3-1b connect, the end set light hole of the first cylindrical body portion 3-1a
3-1c, fluorescence excitation light source 3-3, the first collimation lens 3-4, the first optical filter 3-5, the first condenser lens 3-6 are arranged first
In lens barrel ontology 3-1, the first chassis 3-2 is fixed on the first discshaped body portion 3-1b, fluorescence excitation light source 3-3, the first collimation
Lens 3-4, the first optical filter 3-5, the first condenser lens 3-6 are coaxially arranged.
Fluoroscopic examination component 4 includes the second lens barrel, is arranged in the second lens barrel and edge enters light direction is set gradually second
Collimation lens 4-3, the second optical filter 4-4, photo-electric conversion element 4-5, the second collimation lens 4-3, the second optical filter 4-4, photoelectricity
Conversion element 4-5 is coaxially arranged, and second lens barrel one end is equipped with incidence hole 4-1c, and motor turntable 2-3 is equipped with and incidence hole 4-1c
Matched transmitting light enters hole.Preferably, photo-electric conversion element 4-5 is two pole of photodiode, photomultiplier or photoelectricity snowslide
Pipe.Sample in the 1-1 of example reaction pond is when carrying out PCR reactions, meeting when reagent is irradiated by the exciting light of particular range of wavelengths
Launch the transmitting light of another particular range of wavelengths of more long-wave band, the transmitting light of this specific band is fixed on example reaction
After the transmitting optical delivery fibers 7 of pond 1-1 side walls receives, be transmitted to fluoroscopic examination component 4, fluoroscopic examination component 4 receive it is glimmering
After optical signal, after the second collimation lens 4-3 collimations, photo-electric conversion element is arrived after the second optical filter 4-4 filters out veiling glare signal
Realize that the conversion of photosignal, the ultra-weak electronic signal converted out are transmitted on circuit board by preliminary amplification, complete glimmering on 4.5
The acquisition of light.
In this example, the second lens barrel includes the second lens barrel ontology 4-1 and the second chassis 4-2, and the second lens barrel ontology 4-1 includes connecting
The the second cylindrical body portion 4-1a and the second discshaped body portion 4-1b connect, the end set incidence hole of the second cylindrical body portion 4-1a
4-1c, photo-electric conversion element 4-5, the second collimation lens 4-3, the second optical filter 4-4 are arranged in the second lens barrel ontology 4-1, the
Two chassis 4-2 are fixed on the second discshaped body portion 4-1b.
In order to advanced optimize the implementation result of the present invention, fixed in motor and be equipped with alignment sensor 8 on disk 2-1, with
Positioning when stepper motor 2-2 resets, the alignment sensor 8 are connect with 5 signal of control device.
It is fixed in motor and is equipped with optical fiber fixed part 2-4 on disk 2-1, optical fiber fixed part 2-4 is in the form of annular discs, excites optical transport light
Fibre 6, transmitting optical delivery fiber 7 extend to exciting light optical fiber fixed hole position, transmitting light fiber-optic fixing-hole through optical fiber fixed part 2-4
Position.
The foregoing examples are merely illustrative of the technical concept and features of the invention, its object is to allow the person skilled in the art to be
It cans understand the content of the present invention and implement it accordingly, it is not intended to limit the scope of the present invention.It is all smart according to the present invention
The equivalent transformation or modification that refreshing essence is done, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of PCR real-time fluorescence detection systems of multichannel point detection, including thermocirculator (1), the thermocirculator
(1) several example reaction ponds (1-1) are equipped with；
It is characterized in that, further including：
Sample changeover module (2), including motor fix disk (2-1), stepper motor (2-2) and motor turntable (2-3), the step
Stepper motor (2-2) is fixed on that the motor fixes disk one end (2-1) and output shaft extends to the motor to fix disk (2-1) another
End, the motor turntable (2-3) are sequentially connected with the stepper motor (2-2) and drive backspin in the stepper motor (2-2)
Turn, the motor fix disk (2-1) be equipped with the exciting light optical fiber fixed hole position consistent with example reaction pond (1-1) number and
Emit light fiber-optic fixing-hole position, the exciting light optical fiber fixed hole position is corresponding with transmitting light fiber-optic fixing-hole position and respectively along institute
It states motor and fixes disk (2-1) circumferentially, each described example reaction pond (1-1) side wall is equipped with excitation optical delivery fiber (6)
With transmitting optical delivery fiber (7), the excitation optical delivery fiber (6), transmitting optical delivery fiber (7) are connected to mutual corresponding institute
State exciting light optical fiber fixed hole position, transmitting light fiber-optic fixing-hole position；
Multiple excitation light source components (3) are arranged on the motor turntable (2-3), when the motor turntable (2-3) rotates
Afterwards, the excitation light source component (3) is corresponding with the exciting light optical fiber fixed hole position is swashed with being transmitted into example reaction pond (1-1)
It shines；
Multiple fluoroscopic examination components (4) are arranged on the motor turntable (2-3), when the motor turntable (2-3) rotates
Afterwards, the fluoroscopic examination component (4) and the transmitting light fiber-optic fixing-hole position are corresponding to acquire the hair in example reaction pond (1-1)
Penetrate light；
And control device (5), the thermocirculator (1), sample changeover module (2), laser light source module (3), fluoroscopic examination
Component (4) is electrically connected with the control device (5).
2. the PCR real-time fluorescence detection systems of multichannel point detection according to claim 1, it is characterised in that：It is described to swash
The source component (3) that shines includes the first lens barrel, the fluorescence excitation light source (3-3) that is arranged in first lens barrel and along light extraction side
To the first collimation lens (3-4), the first optical filter (3-5) and the first condenser lens (3-6) set gradually, first lens barrel
One end is equipped with light hole (3-1c), and the motor turntable (2-3) is equipped with and the light hole (3-1c) matched laser light source
Hole.
3. the PCR real-time fluorescence detection systems of multichannel point detection according to claim 2, it is characterised in that：Described
One lens barrel includes the first lens barrel ontology (3-1) and the first chassis (3-2), and the first lens barrel ontology (3-1) includes the connected
One cylindrical body portion (3-1a) and the first discshaped body portion (3-1b), the end set institute in first cylindrical body portion (3-1a)
Light hole (3-1c) is stated, the fluorescence excitation light source (3-3), the first collimation lens (3-4), the first optical filter (3-5), first gather
In the first lens barrel ontology (3-1), first chassis (3-2) is fixed on first plate-like for focus lens (3-6) setting
On body part (3-1b).
4. the PCR real-time fluorescence detection systems of multichannel point detection according to claim 2, it is characterised in that：It is described glimmering
Phot-luminescence source (3-3) is LED light source.
5. the PCR real-time fluorescence detection systems of multichannel point detection according to claim 1, it is characterised in that：It is described glimmering
Photodetection assembly (4) includes the second lens barrel, be arranged in second lens barrel and it is accurate along enter that light direction sets gradually second
Straight lens (4-3), the second optical filter (4-4), photo-electric conversion element (4-5), second lens barrel one end are equipped with incidence hole (4-
1c), the motor turntable (2-3) is equipped with enters hole with the matched transmitting light of the incidence hole (4-1c).
6. the PCR real-time fluorescence detection systems of multichannel point detection according to claim 5, it is characterised in that：Described
Two lens barrels include the second lens barrel ontology (4-1) and the second chassis (4-2), and the second lens barrel ontology (4-1) includes the connected
Two cylindrical body portions (4-1a) and the second discshaped body portion (4-1b), the end set institute in second cylindrical body portion (4-1a)
Incidence hole (4-1c) is stated, second collimation lens (4-3), the second optical filter (4-4), photo-electric conversion element (4-5) are sequentially set
It sets in the second lens barrel ontology (4-1), second chassis (4-2) is fixed on second discshaped body portion (4-1b)
On.
7. the PCR real-time fluorescence detection systems of multichannel point detection according to claim 5, it is characterised in that：The light
Electric transition element (4-5) is photodiode, photomultiplier or photoelectricity avalanche diode.
8. the PCR real-time fluorescence detection systems of multichannel point detection according to claim 1, it is characterised in that：The control
Device (5) processed includes fluorescence control circuit board (5-1) and motor driver (5-2), and the fluorescence control circuit board (5-1) passes through
The motor driver (5-2) controls stepper motor (2-2) work, the thermocirculator (1), laser light source module
(3), fluoroscopic examination component (4) is respectively connected to the fluorescence control circuit board (5-1).
9. the PCR real-time fluorescence detection systems of multichannel point detection according to claim 1, it is characterised in that：The electricity
Machine fixes disk (2-1) and is equipped with alignment sensor (8), positioning when being resetted for the stepper motor (2-2), the positioning
Sensor (8) is connect with the control device (5) signal.
10. the PCR real-time fluorescence detection systems of multichannel point detection according to claim 1, it is characterised in that：The electricity
Machine fixes disk (2-1) and is equipped with optical fiber fixed part (2-4), and the optical fiber fixed part (2-4) is in the form of annular discs, the excitation optical transport
Optical fiber (6), transmitting optical delivery fiber (7) extend to the exciting light optical fiber fixed hole position, hair through the optical fiber fixed part (2-4)
Penetrate light fiber-optic fixing-hole position.
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| CN113670873A (en) * | 2021-08-18 | 2021-11-19 | 中国科学院合肥物质科学研究院 | Fluorescence detection system |
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| CN112345503A (en) * | 2020-10-30 | 2021-02-09 | 上海世艾生物科技有限公司 | Multiple fluorescence detection device |
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| CN113789261A (en) * | 2021-11-18 | 2021-12-14 | 深圳市刚竹智造科技有限公司 | Rotary wheel type optical assembly and rotary wheel type PCR optical system |
| CN114113025A (en) * | 2021-12-21 | 2022-03-01 | 中元汇吉生物技术股份有限公司 | Multi-channel fluorescence scanning device |
| CN114480111A (en) * | 2022-02-15 | 2022-05-13 | 深圳阿斯克医疗有限公司 | Real-time fluorescence quantitative PCR instrument |
| CN116008580A (en) * | 2023-02-20 | 2023-04-25 | 广州科方生物技术股份有限公司 | Automatic fluorescence detection analyzer and fluorescence detection method |
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