CN112345503A - Multiple fluorescence detection device - Google Patents
Multiple fluorescence detection device Download PDFInfo
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- CN112345503A CN112345503A CN202011188679.3A CN202011188679A CN112345503A CN 112345503 A CN112345503 A CN 112345503A CN 202011188679 A CN202011188679 A CN 202011188679A CN 112345503 A CN112345503 A CN 112345503A
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- lens
- laser light
- sample
- annular
- detection device
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6471—Special filters, filter wheel
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
Abstract
The invention discloses a multiple fluorescence detection device, aiming at a sample, comprising: a lens positioned directly above the sample and aligned with the sample; the annular lighting device is sleeved on the periphery of the lens and is annularly provided with a plurality of luminous points; a plurality of laser light sources that emit laser light, respectively; optical fibers connecting the light emitting points of the annular lighting device and the laser light sources; a camera positioned right above the lens and aligned with the lens for collecting fluorescent signals; a filter wheel horizontally arranged between the camera and the lens and carrying a plurality of filters corresponding to different fluorescent substance emission wavelengths in an annular array; and the motor drives the filter wheel to rotate so as to enable one filter to be positioned right above the lens. The cross among the fluorescence is reduced, and the detection sensitivity and accuracy of the fluorescence are effectively improved.
Description
Technical Field
The invention relates to the field of fluorescence detection, in particular to a multiple fluorescence detection device.
Background
At present, in most nucleic acid detection fields such as judicial identification, DNA identification of criminals in forensic science, clinical genetic variation detection, identification of viruses and bacteria, most methods label nucleic acid with a fluorophore, and carry out result judgment by combining the fluorophore with target nucleic acid.
Disclosure of Invention
The invention aims to provide a multiple fluorescence detection device, which reduces the cross among fluorescence and effectively improves the detection sensitivity and accuracy of the fluorescence.
The technical scheme for realizing the purpose is as follows:
a multiplex fluorescence detection device, aligned to a sample, comprising:
a lens positioned directly above the sample and aligned with the sample;
the annular lighting device is sleeved on the periphery of the lens or is uniformly embedded in the lens, and a plurality of light-emitting points are annularly distributed on the annular lighting device;
a plurality of laser light sources that emit laser light, respectively;
optical fibers connecting the light emitting points of the annular lighting device and the laser light sources;
a camera positioned right above the lens and aligned with the lens for collecting fluorescent signals;
a filter wheel horizontally arranged between the camera and the lens and carrying a plurality of filters corresponding to different fluorescent substance emission wavelengths in an annular array; and
and the motor drives the filter wheel to rotate so as to enable one filter to be positioned right above the lens.
Preferably, the number of the laser light sources is 4, and the number of the filters in the filter wheel is 6.
Preferably, the ring-shaped lighting device includes: the annular sleeve is sleeved on the periphery of the lens, and the luminous points are annularly fixed on the annular sleeve;
one end of the optical fiber is respectively connected with each laser light source, the other end of the optical fiber is evenly divided into a plurality of ends, and the ends are fixedly connected with the light-emitting points one by one.
Preferably, the method further comprises the following steps: and the output of the motor is controlled according to the type of the fluorescent substances on the sample, so that the optical filter corresponding to the emission wavelength of the fluorescent substances is automatically switched to the singlechip at the position opposite to the lens.
The invention has the beneficial effects that: according to the invention, the optical filters are switched through the rotation of the optical filter wheel, so that the cross among the fluorescent lights is reduced, and the detection sensitivity and accuracy of the fluorescent lights are improved to a great extent. By using the annular lighting device with a plurality of annularly distributed luminous points, the space material is saved, and simultaneously, the light can be uniformly irradiated on the sample.
Drawings
FIG. 1 is a structural view of a multiple fluorescence detecting apparatus of the present invention;
FIG. 2 is a schematic view of a filter wheel according to the present invention;
fig. 3 is a structural view of the ring lighting device of the present invention.
Detailed Description
The invention will be further explained with reference to the drawings.
Referring to fig. 1-3, the multiple fluorescence detection device of the present invention, aligned with a sample 100, includes: the device comprises a lens 1, a ring-shaped lighting device 2, a plurality of laser light sources 3, an optical fiber 4, a camera 5, a filter wheel 6 and a motor 7.
Lens 1 is positioned directly above sample 100 and aligned with sample 100. The annular lighting device 2 is sleeved on the periphery of the lens 100 or is uniformly embedded in the lens, and a plurality of luminous points 21 are annularly distributed. The laser light sources 3 emit laser light, respectively. The optical fiber 4 connects each light emitting point 21 of the ring-shaped illumination device 2 and each laser light source 3. The ring-shaped lighting device 2 includes: an annular sleeve 22 which is sleeved on the periphery of the lens 1, and a luminous point 21 which is annularly fixed on the annular sleeve 22. One end of the optical fiber 4 is respectively connected with each laser light source 3, the other end is evenly divided into a plurality of end heads, and each end head is fixedly connected with each luminous point 21 one by one.
A camera 5 is positioned directly above the lens 1 and aimed at the lens 1 for collecting the fluorescence signal.
The filter wheel 6 is horizontally disposed between the camera 5 and the lens 1, and a plurality of filters 61 corresponding to different emission wavelengths of the fluorescent dye are mounted in a circular array. The motor 7 drives the filter wheel 6 to rotate so that one of the filters is positioned right above the lens 1. The mode that the motor 7 drives the filter wheel 6 to rotate can be directly driven, and can also be driven through a gear connection structure. The number of the laser light sources 3 and the number of the filters 61 are designed according to actual conditions, and in the embodiment, the number of the laser light sources 3 is 4, and the number of the filters 61 in the filter wheel 6 is 6.
When a certain laser light source 3 is turned on, laser is transmitted to each light-emitting point 21 through an optical fiber, emitted light is irradiated on a sample 100, a fluorescent substance on the sample 100 is excited by the laser to emit specific fluorescent light, the fluorescent light is reflected upwards through the lens 1, the optical filter 61 corresponding to the emission wavelength of the fluorescent substance is rotated to a position opposite to the lens 1, and after the fluorescent light passes through the optical filter 61, a fluorescent signal is collected by the camera 5.
In order to improve the automatic control performance of the invention, the single chip microcomputer is used for controlling the output of the motor 7, so that the filter wheel 6 rotates by the same fixed angle each time, and the single chip microcomputer controls the motor 7 to output for multiple times according to the type of the fluorescent substances on the sample 100, so that the filter 61 corresponding to the fluorescent substances automatically rotates to the position opposite to the lens 1.
The above embodiments are provided only for illustrating the present invention and not for limiting the present invention, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention, and therefore all equivalent technical solutions should also fall within the scope of the present invention, and should be defined by the claims.
Claims (4)
1. A multiplex fluorescence detection device for registering a sample, comprising:
a lens positioned directly above the sample and aligned with the sample;
the annular lighting device is sleeved on the periphery of the lens or is uniformly embedded in the lens, and a plurality of light-emitting points are annularly distributed on the annular lighting device;
a plurality of laser light sources that emit laser light, respectively;
optical fibers connecting the light emitting points of the annular lighting device and the laser light sources;
a camera positioned right above the lens and aligned with the lens for collecting fluorescent signals;
a filter wheel horizontally arranged between the camera and the lens and carrying a plurality of filters corresponding to different fluorescent substance emission wavelengths in an annular array; and
and the motor drives the filter wheel to rotate so as to enable one filter to be positioned right above the lens.
2. The multiple fluorescence detection device of claim 1, wherein the number of laser light sources is 4 and the number of filters in the filter wheel is 6.
3. The multiplex fluorescence detection device of claim 1, wherein the annular illumination device comprises: the annular sleeve is sleeved on the periphery of the lens, and the luminous points are annularly fixed on the annular sleeve;
one end of the optical fiber is respectively connected with each laser light source, the other end of the optical fiber is evenly divided into a plurality of ends, and the ends are fixedly connected with the light-emitting points one by one.
4. The multiplex fluorescence detection device of claim 1, further comprising: and the output of the motor is controlled according to the type of the fluorescent substances on the sample, so that the optical filter corresponding to the emission wavelength of the fluorescent substances is automatically switched to the singlechip at the position opposite to the lens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011188679.3A CN112345503A (en) | 2020-10-30 | 2020-10-30 | Multiple fluorescence detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011188679.3A CN112345503A (en) | 2020-10-30 | 2020-10-30 | Multiple fluorescence detection device |
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| CN112345503A true CN112345503A (en) | 2021-02-09 |
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| CN202011188679.3A Pending CN112345503A (en) | 2020-10-30 | 2020-10-30 | Multiple fluorescence detection device |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106723A (en) * | 2011-03-17 | 2011-06-29 | 中国科学院自动化研究所 | Fluorescence molecule imaging device |
| CN104121541A (en) * | 2014-07-18 | 2014-10-29 | 合肥流明新能源科技有限公司 | LED street lamp capable of automatically removing dust |
| CN104677870A (en) * | 2015-02-06 | 2015-06-03 | 余家昌 | Superminiaturization multi-channel real-time fluorescent spectrum detector |
| CN207438478U (en) * | 2017-11-30 | 2018-06-01 | 安徽西马新能源技术有限公司 | A kind of high-efficiency heat radiating LED car light |
| CN108642158A (en) * | 2018-06-19 | 2018-10-12 | 苏州雅睿生物技术有限公司 | A kind of PCR real-time fluorescence detection systems of multichannel point detection |
| CN110966566A (en) * | 2019-12-06 | 2020-04-07 | 常州市丰祐照明科技有限公司 | LED car light that radiating effect is good |
| CN211263187U (en) * | 2019-11-22 | 2020-08-14 | 苏州丹宝医疗科技有限公司 | Optical fiber-free multi-channel CCD fluorescence detection system |
| CN111830002A (en) * | 2020-08-10 | 2020-10-27 | 杭州天微基因科技有限公司 | Multi-channel detection system for real-time nucleic acid fluorescence detection |
-
2020
- 2020-10-30 CN CN202011188679.3A patent/CN112345503A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106723A (en) * | 2011-03-17 | 2011-06-29 | 中国科学院自动化研究所 | Fluorescence molecule imaging device |
| CN104121541A (en) * | 2014-07-18 | 2014-10-29 | 合肥流明新能源科技有限公司 | LED street lamp capable of automatically removing dust |
| CN104677870A (en) * | 2015-02-06 | 2015-06-03 | 余家昌 | Superminiaturization multi-channel real-time fluorescent spectrum detector |
| CN207438478U (en) * | 2017-11-30 | 2018-06-01 | 安徽西马新能源技术有限公司 | A kind of high-efficiency heat radiating LED car light |
| CN108642158A (en) * | 2018-06-19 | 2018-10-12 | 苏州雅睿生物技术有限公司 | A kind of PCR real-time fluorescence detection systems of multichannel point detection |
| CN211263187U (en) * | 2019-11-22 | 2020-08-14 | 苏州丹宝医疗科技有限公司 | Optical fiber-free multi-channel CCD fluorescence detection system |
| CN110966566A (en) * | 2019-12-06 | 2020-04-07 | 常州市丰祐照明科技有限公司 | LED car light that radiating effect is good |
| CN111830002A (en) * | 2020-08-10 | 2020-10-27 | 杭州天微基因科技有限公司 | Multi-channel detection system for real-time nucleic acid fluorescence detection |
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Application publication date: 20210209 |
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