CN111830002A - Multi-channel detection system for real-time nucleic acid fluorescence detection - Google Patents
Multi-channel detection system for real-time nucleic acid fluorescence detection Download PDFInfo
- Publication number
- CN111830002A CN111830002A CN202010794059.8A CN202010794059A CN111830002A CN 111830002 A CN111830002 A CN 111830002A CN 202010794059 A CN202010794059 A CN 202010794059A CN 111830002 A CN111830002 A CN 111830002A
- Authority
- CN
- China
- Prior art keywords
- detection
- filter
- nucleic acid
- real
- detection system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6471—Special filters, filter wheel
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6478—Special lenses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
Abstract
The invention discloses a multi-channel detection system for real-time nucleic acid fluorescence detection, which comprises a temperature control module, a hot cover, a filter wheel, a Y-shaped fluorescence detection optical fiber, a motion scanning module and a photoelectric detection module, wherein the temperature control module is connected with the hot cover; the exciting light LED emits exciting light, the exciting light is collimated by the collimating lens, the exciting light is filtered to light waves with specific wavelengths by the exciting light filter, the light waves pass through the Y-shaped fluorescent detection optical fiber, are focused by the lens and irradiate a sample in the test tube, and a fluorescent signal is excited; the fluorescent signal is focused on the Y-shaped fluorescent detection optical fiber through the lens, an invalid signal is filtered out through the emission optical filter, and the fluorescent signal is converted into an electric signal through the collimating lens focusing and photoelectric detection module; the filter wheel is driven by the motor to rotate by a specific angle so as to switch the optical filter component with a specific wavelength to realize a multiband detection function; the detection of the hole sites of the multiple test tubes is realized by driving the Y-shaped fluorescence detection optical fiber to move through the motion scanning module.
Description
Technical Field
The invention belongs to the field of molecular diagnosis, and relates to a multi-channel detection system for real-time nucleic acid fluorescence detection.
Background
Multiplex PCR (multiplex PCR), also called multiplex PCR or multiplex PCR, is a PCR reaction in which two or more pairs of primers are added to the same PCR reaction system to simultaneously amplify multiple nucleic acid fragments, and the reaction principle, reaction reagents and operation process are the same as those of ordinary PCR. Multiplex PCR (multiplex PCR), in which a plurality of pairs of primers and a plurality of fluorescent probes are added to a reaction system for a plurality of target DNAs by using the specificity of the combination of the primers and the fluorescent probes with the target DNAs, and the plurality of target DNAs are detected by one-time real-time fluorescent quantitative PCR. The method overcomes the defects of complicated operation, difficult quantification and easy pollution of the common PCR, improves the detection flux and reliability, and makes the real-time fluorescence quantitative PCR become practical. In multiplex PCR, in order to avoid the mutual mixing of fluorescence of different fluorescent probes, fluorescent reporter groups with different excitation wavelengths and detection wavelengths are selected to synthesize the fluorescent probes. This also puts the demand of multiple fluorescence channels on the fluorescence detection system of the real-time fluorescence quantitative PCR instrument.
At present, the domestic multi-fluorescence channel nucleic acid amplification detection technology mainly adopts an optical detection element to scan an amplification sample one by one at the bottom of a test tube module to obtain fluorescence data, the method adopts the movement of the bottom of the test tube in two directions of an X axis and a Y axis to collect fluorescence signals in the test tube, and fluorescence channels with different spectrums are switched by a channel switching device, and the scheme has the following defects:
the bottom scanning detection scheme requires corresponding detection hole sites to be processed on the refrigeration heating part, and the customization cost is high.
Because the fluorescent signal belongs to a weak light signal and needs to reduce the detection distance to the maximum extent to enhance the signal, the bottom scanning scheme has special thickness to the radiator, needs to adopt a special heat pipe radiator, and has high process difficulty and high cost.
Due to the fact that the detection holes are formed in the refrigerating and heating component, the thickness of the radiator is insufficient, and the like, the temperature performance of the whole amplification module is reduced, and therefore a PCR amplification experiment is influenced.
Due to the thickness of the heat sink, the bottom scanning scheme requires optical light guiding measures, which greatly increase the cost of the detection system.
By adopting the bottom scanning scheme, the optical detection head is easily polluted by environmental dust, so that the optical detection performance of the detection equipment is reduced.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides a multi-channel detection system for real-time nucleic acid fluorescence detection, which does not need to customize a heating and refrigerating part, has no special requirement on the thickness of a radiator, can greatly improve the temperature performance of a nucleic acid amplification module, simultaneously cancels unnecessary light guide measures and reduces the overall cost of equipment. Because the optical detection head is arranged upside down at the top of the test tube and is not easy to be polluted by environmental dust, the long-term stability of the optical detection performance is ensured.
A multi-channel detection system for real-time nucleic acid fluorescence detection, comprising:
a temperature control module having one or more test tube wells;
a thermal cover provided with one or more detection holes;
the light source emits light which is filtered into exciting light with corresponding wavelength through the exciting light filter, the exciting light enters the test tube through the optical fiber, a sample in the test tube is excited to emit fluorescence, the fluorescence is incident to the emitting light filter through the optical fiber, and the filter wheel rotates by a specific angle to detect fluorescence signals of different wave bands;
the optical detection head is a Y-shaped light guide optical fiber, one end of the optical detection head is positioned at the top of the test tube, the other end of the optical detection head is divided into two ends, one end of the optical detection head is fixed above the exciting light filter of the filter wheel, and the other end of the optical detection head is fixed above the emission light filter of the filter wheel;
the scanning motion module is used for driving one end of the Y-shaped fluorescence detection optical fiber, which is positioned at the top of the test tube, to perform motion scanning, and can sequentially detect a plurality of test tube hole sites;
a photoelectric detection module for detecting the fluorescent signal incident through the emission filter.
Preferably, the excitation light and the excitation light filters of the filter wheel are coaxially mounted in a one-to-one correspondence manner.
Preferably, the specific waveband excitation light filter and the specific waveband emission light filter of the filter wheel are arranged in a 180-degree manner and are arranged in a matched mode.
Preferably, a focusing lens is arranged in the detection hole of the thermal cover.
Preferably, a collimating lens is arranged between the excitation light of the filter wheel and the excitation light filter.
Preferably, a collimating lens is arranged between the emission filter of the filter wheel and the photoelectric detection module.
Preferably, a fiber head fixing seat for fixing the Y-shaped fluorescence detection fiber is arranged above the filter wheel.
Preferably, a photoelectric module fixing seat is arranged below the filter wheel.
Preferably, the multispectral detection system comprises a motor drive assembly connected to the filter wheel.
Preferably, the scanning motion module comprises a linear motion mode and a rotation mode.
Compared with the prior art, the gain effect of the invention is as follows:
the heating and refrigerating module is not required to be processed and customized with the detection hole, so that the equipment cost is greatly reduced.
And a special heat pipe radiator is not needed, so that the cost is saved, and the difficulty of the product processing technology is reduced.
Light guide measures are not needed, and the equipment cost is greatly reduced.
The temperature performance of the equipment is effectively improved.
The optical detection performance of the device is more stable.
Drawings
FIG. 1 is a perspective view of the overall structure of a multi-channel detection system for real-time nucleic acid fluorescence detection.
FIG. 2 is a top view of a multi-channel detection system for real-time nucleic acid fluorescence detection.
FIG. 3 is a cross-sectional view of a multi-channel detection system for real-time nucleic acid fluorescence detection.
Fig. 4 is a perspective view of the filter wheel.
FIG. 5 is a top view of the filter wheel.
FIG. 6 is a cross-sectional view of the filter wheel.
Fig. 7 is a perspective view of a temperature control module.
FIG. 8 is a top view of the thermal cap.
Fig. 9 is a perspective view of a motion scanning module.
Detailed Description
The technical embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention.
Referring to FIGS. 1 and 3, the present invention discloses a multi-channel detection system for real-time fluorescence detection of nucleic acids, comprising: the device comprises a temperature control module (1), a hot cover (2), a filter wheel (3), a Y-shaped fluorescence detection optical fiber (4), a scanning motion module (5) and a photoelectric detection module (6).
The temperature control module (1) comprises a plurality of test tube wells as shown in FIG. 7.
The thermal cover (2) is provided with a plurality of detection holes as shown in FIG. 8.
A filter wheel (3) comprising six excitation light filters (7), six excitation light LEDs (9), and six emission light filters (10) as shown in fig. 5 and 6; a collimating lens (8) is arranged between the exciting light and the exciting light filter; the exciting light LEDs (9) correspond to the exciting light filters (7) one by one; the specific wavelength excitation light filter (7) and the specific wavelength emission light filter (10) are installed at an angle of 180 degrees.
As shown in fig. 3, one end of the Y-shaped fluorescence detection fiber (4) is located at the top of the test tube and connected to the motion module (5), and the other end is fixed to the optical fiber fixing base (12) of the filter wheel.
A scanning motion module (5) composed of a synchronous belt (24), a linear guide rail (25), a slide block (18) and a motor (26) in the X-axis direction as shown in FIG. 9; consists of a screw rod slide block (17), a screw rod (22) and a motor (23) in the Y-axis direction
The photoelectric detection module (6) is fixed on the photoelectric module fixing seat (13) as shown in fig. 3, and the photoelectric detection module (6) and the emission light filter (10) are provided with collimating lenses (11).
Now, the optical signal transduction process of the information nucleic acid amplification optical detection system will be described in further detail with reference to FIG. 3.
The multichannel detection system is started, exciting light is emitted by an exciting light LED (9), the exciting light is collimated through a collimating lens (8), the exciting light is filtered to light waves with specific wavelengths through an exciting light filter (7), the light waves pass through a Y-shaped fluorescence detection optical fiber (4), a sample in a test tube (14) is focused and irradiated through a lens (15), and a fluorescence signal is excited. The fluorescent signal is focused on the Y-shaped fluorescent detection optical fiber (4) through the lens (15), invalid signals are filtered out through the emission optical filter (10), and the fluorescent signal is converted into an electric signal through the collimating lens (11) for focusing and the photoelectric detection module (6). As shown in FIG. 5, the multichannel detection system uses a motor (16) to drive a synchronous belt (20) to drive a synchronous belt wheel (19) to drive a filter wheel (3) to rotate by a specific angle, so that a filter component with a specific wavelength is switched to realize a multiband detection function. As shown in figures 1 and 2, the multi-channel detection system drives a Y-shaped fluorescence detection optical fiber (4) to move through a motion scanning module (5) to realize the detection of the hole sites of the multiple test tubes.
It is to be understood that: although the present invention has been described in detail, the present invention is only for the purpose of illustration and not limitation, and any invention that does not depart from the spirit of the present invention falls within the scope of the present invention.
Claims (10)
1. A multi-channel detection system for real-time nucleic acid fluorescence detection, comprising:
a temperature control module having one or more test tube wells;
a thermal cover provided with one or more detection holes;
the light source emits light which is filtered into exciting light with corresponding wavelength through the exciting light filter, the exciting light enters the test tube through the optical fiber, a sample in the test tube is excited to emit fluorescence, the fluorescence is incident to the emitting light filter through the optical fiber, and the filter wheel rotates by a specific angle to detect fluorescence signals of different wave bands;
the optical detection head is a Y-shaped light guide optical fiber, one end of the optical detection head is positioned at the top of the test tube, the other end of the optical detection head is divided into two ends, one end of the optical detection head is fixed above the exciting light filter of the filter wheel, and the other end of the optical detection head is fixed above the emission light filter of the filter wheel;
the scanning motion module is used for driving one end of the Y-shaped fluorescence detection optical fiber, which is positioned at the top of the test tube, to perform motion scanning, and can sequentially detect a plurality of test tube hole sites;
a photoelectric detection module for detecting the fluorescent signal incident through the emission filter.
2. The multi-channel detection system for real-time nucleic acid fluorescence detection of claim 1, wherein the excitation light and excitation light filters of the filter wheel are coaxially mounted in a one-to-one correspondence.
3. The multi-channel detection system for real-time nucleic acid fluorescence detection of claim 1, wherein the specific wavelength band excitation light filter and the specific wavelength band emission light filter of the filter wheel are mounted at 180 ° in a paired arrangement.
4. The multi-channel detection system for real-time nucleic acid fluorescence detection of claim 1, wherein a focusing lens is disposed within the detection well of the thermal cap.
5. The multi-channel detection system for real-time nucleic acid fluorescence detection of claim 1, wherein a collimating lens is disposed between the excitation light and the excitation light filter of the filter wheel.
6. The multi-channel detection system for real-time nucleic acid fluorescence detection according to claim 1, wherein a collimating lens is disposed between the emission filter of the filter wheel and the photodetection module.
7. The multi-channel detection system for real-time nucleic acid fluorescence detection according to claim 1, wherein a fiber tip holder for holding the Y-shaped fluorescence detection fiber is disposed above the filter wheel.
8. The multi-channel detection system for real-time nucleic acid fluorescence detection according to claim 1, wherein a photo module holder is disposed below the filter wheel.
9. The multi-channel detection system for real-time nucleic acid fluorescence detection of claim 1, wherein the multi-spectral detection system comprises a motor drive assembly coupled to a filter wheel.
10. The multi-channel detection system for real-time nucleic acid fluorescence detection of claim 1, wherein the scanning motion module comprises linear motion and rotational motion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010794059.8A CN111830002A (en) | 2020-08-10 | 2020-08-10 | Multi-channel detection system for real-time nucleic acid fluorescence detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010794059.8A CN111830002A (en) | 2020-08-10 | 2020-08-10 | Multi-channel detection system for real-time nucleic acid fluorescence detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111830002A true CN111830002A (en) | 2020-10-27 |
Family
ID=72919946
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010794059.8A Pending CN111830002A (en) | 2020-08-10 | 2020-08-10 | Multi-channel detection system for real-time nucleic acid fluorescence detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111830002A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112345503A (en) * | 2020-10-30 | 2021-02-09 | 上海世艾生物科技有限公司 | Multiple fluorescence detection device |
| CN113670873A (en) * | 2021-08-18 | 2021-11-19 | 中国科学院合肥物质科学研究院 | Fluorescence detection system |
| CN114181823A (en) * | 2022-02-16 | 2022-03-15 | 苏州雅睿生物技术股份有限公司 | PCR instrument with multiple temperature control modules and asynchronous optional channels and detection method thereof |
| CN114460056A (en) * | 2022-02-16 | 2022-05-10 | 苏州雅睿生物技术股份有限公司 | Linear scanning type fluorescence detection system based on no optical fiber and PCR instrument |
| WO2022160726A1 (en) * | 2021-01-29 | 2022-08-04 | 广东润鹏生物技术有限公司 | Multi-channel fluorescence quantitative detection device and molecular diagnosis platform |
| CN115747035A (en) * | 2023-01-05 | 2023-03-07 | 苏州和迈精密仪器有限公司 | Continuous scanning monitoring analysis system based on discrete three-dimensional fluorescence technology |
-
2020
- 2020-08-10 CN CN202010794059.8A patent/CN111830002A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112345503A (en) * | 2020-10-30 | 2021-02-09 | 上海世艾生物科技有限公司 | Multiple fluorescence detection device |
| WO2022160726A1 (en) * | 2021-01-29 | 2022-08-04 | 广东润鹏生物技术有限公司 | Multi-channel fluorescence quantitative detection device and molecular diagnosis platform |
| CN113670873A (en) * | 2021-08-18 | 2021-11-19 | 中国科学院合肥物质科学研究院 | Fluorescence detection system |
| CN114181823A (en) * | 2022-02-16 | 2022-03-15 | 苏州雅睿生物技术股份有限公司 | PCR instrument with multiple temperature control modules and asynchronous optional channels and detection method thereof |
| CN114460056A (en) * | 2022-02-16 | 2022-05-10 | 苏州雅睿生物技术股份有限公司 | Linear scanning type fluorescence detection system based on no optical fiber and PCR instrument |
| CN115747035A (en) * | 2023-01-05 | 2023-03-07 | 苏州和迈精密仪器有限公司 | Continuous scanning monitoring analysis system based on discrete three-dimensional fluorescence technology |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111830002A (en) | Multi-channel detection system for real-time nucleic acid fluorescence detection | |
| CN107746806B (en) | Real-time fluorescence quantitative PCR instrument | |
| US5538613A (en) | Electrophoresis analyzer | |
| KR101334183B1 (en) | Fluorescence detecting module for microreaction and fluorescence detecting system having the same | |
| EP1228357B1 (en) | Fluorometer with low heat-generating light source | |
| JP3626956B2 (en) | Polycapillary fluorescence detection system | |
| US7109495B2 (en) | Fluorometer with low heat-generating light source | |
| US8309940B2 (en) | Lighting design of high quality biomedical devices | |
| US20100277725A1 (en) | Universal multidetection system for microplates | |
| CN108060068B (en) | Multi-channel fluorescence detection device | |
| CN104568875A (en) | Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system | |
| AU2002336771B2 (en) | Imaging of microarrays using fiber optic exciter | |
| CN110967324A (en) | Optical detection device of multi-channel real-time fluorescence detector | |
| CN212483359U (en) | Multi-channel detection system for real-time nucleic acid fluorescence detection | |
| US20070194247A1 (en) | Compact optical module for fluorescence excitation and detection | |
| CN212748736U (en) | Multi-channel switching device for fluorescence detection based on Photodiode (PD) | |
| CN110018139B (en) | Multicolor fluorescence detection device | |
| CN110935498A (en) | Fluorescence scanning system for PCR instrument | |
| CN210269617U (en) | Optical detection device and capillary electrophoresis apparatus | |
| CN212483358U (en) | Nucleic acid optical detection system based on top scanning detection | |
| CN219099173U (en) | Real-time fluorescence PCR optical detection system | |
| CN112161927A (en) | In-vitro diagnosis and analysis system and optical detection device | |
| CN114062326A (en) | Nucleic acid optical detection system based on top scanning detection | |
| CN215218549U (en) | Fluorescence detection device | |
| CN113092424B (en) | High-flux biochip analyzer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |