CN209778861U - Efficient detection device for MicroRNA - Google Patents
Efficient detection device for MicroRNA Download PDFInfo
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- CN209778861U CN209778861U CN201920399622.4U CN201920399622U CN209778861U CN 209778861 U CN209778861 U CN 209778861U CN 201920399622 U CN201920399622 U CN 201920399622U CN 209778861 U CN209778861 U CN 209778861U
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- fiber bundle
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- 238000001514 detection method Methods 0.000 title claims abstract description 16
- 108700011259 MicroRNAs Proteins 0.000 title claims description 20
- 238000003384 imaging method Methods 0.000 claims abstract description 13
- 239000013307 optical fiber Substances 0.000 claims description 56
- 230000003287 optical effect Effects 0.000 claims description 12
- 238000003556 assay Methods 0.000 claims 4
- 239000000835 fiber Substances 0.000 abstract description 31
- 238000002189 fluorescence spectrum Methods 0.000 abstract description 6
- 108091070501 miRNA Proteins 0.000 abstract description 6
- 239000002679 microRNA Substances 0.000 abstract description 6
- 238000001228 spectrum Methods 0.000 abstract description 5
- 230000015572 biosynthetic process Effects 0.000 abstract description 4
- 230000005284 excitation Effects 0.000 abstract description 4
- 238000005259 measurement Methods 0.000 abstract description 4
- 230000006872 improvement Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000004791 biological behavior Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses an efficient is used for micro RNA's detection device, including tunable laser, leading-in fiber bundle, sample, focusing lens, output fiber, light filter, formation of image spectrum appearance, EMCCD detector and computer, tunable laser one side is equipped with leading-in fiber bundle, the sample is connected to leading-in fiber bundle one end, the sample top is equipped with focusing lens, be equipped with output fiber on the focusing lens, output fiber is equipped with the multiunit, the multiunit output fiber constitutes output fiber bundle; the utility model discloses an utilize tunable laser to carry out monochromatic laser excitation, the narrowband filter of receipt department filters, utilizes imaging spectrometer to gather, received signal simultaneously, can measure (being greater than 100) a plurality of samples simultaneously on the one hand, still can acquire fluorescence spectrum curve in addition, can distinguish interference light, improves the micro RNA measurement accuracy.
Description
Technical Field
The utility model relates to the technical field of medical equipment, especially, relate to an efficient is used for micro RNA's detection device.
Background
MicroRNA is an endogenous single-stranded RNA of about 20-24 nucleotides in length. MicroRNA participates in a series of complex biological behaviors such as cell growth, development, differentiation and death, is closely related to the occurrence and development of diseases in the life process, and has larger challenge for detecting different MicroRNAs with only one base difference due to the short length of mature MicroRNA and the sequence homology of the mature MicroRNA.
At present, the detection method of MicroRNA mainly has real-time fluorescence quantitative PCR, Northern blot analysis and microarray chip, however, such detection not only needs expensive instrument and equipment and strict laboratory conditions, the detection process is complicated to operate, the detection time is long, the cost is high, and need the relevant personnel through professional training, it is difficult to develop in the mechanism of general condition, some fluorescent materials that are used for detecting the device of MicroRNA are SYBRGreen II fluorescent dyes and can appear that the exciting light is overlapped with the fluorescence wavelength by a small part, influence the detection precision, and some detection devices can only measure a sample once, and the efficiency is low, therefore, the utility model provides an efficient detection device for MicroRNA, in order to solve the weak point among the prior art.
SUMMERY OF THE UTILITY MODEL
To the above problem, the utility model discloses an utilize tunable laser to carry out monochromatic laser excitation, the narrowband optical filter of receipt department filters, utilizes imaging spectrometer to gather, received signal simultaneously, can measure (being greater than 100) a plurality of samples simultaneously on the one hand, still can acquire fluorescence spectrum curve in addition, can distinguish interference light, improves the micro RNA measurement accuracy.
The utility model provides an efficient is used for micro RNA's detection device, including tunable laser, leading-in fiber bundle, sample, focusing lens, output fiber, light filter, formation of image spectrum appearance, EMCCD detector and computer, tunable laser one side is equipped with leading-in fiber bundle, the sample is connected to leading-in fiber bundle one end, the sample top is equipped with focusing lens, be equipped with output fiber on the focusing lens, output fiber is equipped with the multiunit, the multiunit output fiber bundle is constituteed to output fiber, output fiber bundle one side is equipped with the light filter, light filter one side is equipped with the formation of image spectrum appearance, formation of image spectrum appearance one side is equipped with the EMCCD detector, the computer is connected to the EMCCD detector.
The further improvement lies in that: the leading-in optical fiber bundle is composed of a plurality of groups of leading-in optical fibers, and one ends of the groups of leading-in optical fibers are connected with samples.
The further improvement lies in that: the leading-in optical fiber, the output optical fiber, the sample and the focusing lens are the same in number.
The further improvement lies in that: and one end of the leading-in optical fiber bundle, which is close to the tunable laser, and one end of the output optical fiber bundle, which is close to the optical filter, are both provided with optical fiber fixing sleeves.
The further improvement lies in that: the optical fiber bundle fixing sleeve is used for fixing the output optical fiber and the leading-in optical fiber.
The utility model has the advantages that: the utility model provides a through utilizing tunable laser to carry out monochromatic laser excitation, the narrowband filter of receipt department filters, utilize the imaging spectrometer to gather simultaneously, received signal, can measure (being greater than 100) a plurality of samples simultaneously on the one hand, still can acquire fluorescence spectrum curve in addition, can distinguish the interference light, improve MicroRNA measurement accuracy, through setting up the intensity that leading-in optic fibre and output optic fibre bundle one end can protect leading-in optic fibre and output optical fibre at leading-in optic fibre bundle and output optic fibre bundle one end, avoid appearing the rupture condition, can improve MicroRNA's detection stability.
Drawings
Fig. 1 is a schematic view of the structure of the present invention.
Wherein: the device comprises a tunable laser 1, a sample 2, a focusing lens 3, an output optical fiber 4, an optical filter 5, an imaging spectrometer 6, an EMCCD detector 7, a computer 8, a lead-in optical fiber 9 and an optical fiber fixing sleeve 10.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
According to fig. 1, this embodiment provides an efficient detection device for micro rna, including tunable laser 1, leading-in fiber bundle, sample 2, focusing lens 3, output fiber 4, optical filter 5, imaging spectrometer 6, EMCCD detector 7 and computer 8, tunable laser 1 one side is equipped with leading-in fiber bundle, sample 2 is connected to leading-in fiber bundle one end, sample 2 top is equipped with focusing lens 3, be equipped with output fiber 4 on focusing lens 3, output fiber 4 is equipped with the multiunit, the multiunit output fiber 4 constitutes the output fiber bundle, output fiber bundle one side is equipped with optical filter 5, optical filter 5 one side is equipped with imaging spectrometer 6, imaging spectrometer 6 one side is equipped with EMCCD detector 7, EMCCD detector 7 connects computer 8.
The leading-in optical fiber bundle is composed of a plurality of groups of leading-in optical fibers 9, and one ends of the groups of leading-in optical fibers 9 are connected with the sample 2. The leading-in optical fiber 9, the output optical fiber 4, the sample 2 and the focusing lens 3 are the same in quantity, the tunable laser 1 emits laser with the wavelength of 497nm, the laser enters the leading-in optical fiber bundle, after beam splitting, a plurality of groups of leading-in optical fibers 9 are arranged, the leading-in optical fibers 9 respectively irradiate a plurality of groups of samples 2, fluorescence excited by different samples 2 is focused to an output optical fiber 4 through respective focusing lenses 3, then the fluorescence is combined into an output optical fiber bundle, and after the fluorescence passes through an optical filter 5, entering an imaging spectrometer 6, becoming a spectrum dimension and a space dimension (corresponding to different samples 2) after light splitting by the imaging spectrometer 6, receiving by an EMCCD detector 7, transmitting to a computer 8 for processing after photoelectric conversion and analog-to-digital conversion, obtaining fluorescence spectra of different samples, analyzing fluorescence spectrum signals to obtain the MicroRNA concentration of the sample to be detected, and an optical fiber fixing sleeve 10 is arranged at one end of the leading-in optical fiber bundle close to the tunable laser 1 and one end of the output optical fiber bundle close to the optical filter 5. The optical fiber bundle fixing sleeve 10 is used for fixing the output optical fiber 4 and the input optical fiber 9, so as to achieve the purpose of simultaneously measuring a plurality of samples 2.
Monochromatic laser excitation is carried out by utilizing the tunable laser 1, the narrow-band filter 5 at the receiving part filters light, the imaging spectrometer 6 is utilized to collect and receive signals, on one hand, a plurality of samples can be measured simultaneously (more than 100), in addition, a fluorescence spectrum curve can be obtained, interference light can be distinguished, the measurement precision of MicroRNA is improved, the intensity of the leading-in optical fiber 9 and the output optical fiber 4 can be protected by arranging the optical fiber fixing sleeve 10 at one end of the leading-in optical fiber bundle and one end of the output optical fiber bundle, the breaking condition is avoided, and the detection stability of MicroRNA can be improved.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. A high-efficiency detection device for MicroRNA is characterized in that: comprises a tunable laser (1), a leading-in optical fiber bundle, a sample (2), a focusing lens (3), an output optical fiber (4), an optical filter (5), an imaging spectrometer (6), an EMCCD detector (7) and a computer (8), one side of the tunable laser (1) is provided with a lead-in optical fiber bundle, one end of the lead-in optical fiber bundle is connected with a sample (2), a focusing lens (3) is arranged above the sample (2), an output optical fiber (4) is arranged on the focusing lens (3), the output optical fibers (4) are provided with a plurality of groups, the output optical fibers (4) form an output optical fiber bundle, one side of the output optical fiber bundle is provided with an optical filter (5), one side of the optical filter (5) is provided with an imaging spectrometer (6), an EMCCD detector (7) is arranged on one side of the imaging spectrometer (6), and the EMCCD detector (7) is connected with a computer (8).
2. The high efficiency assay device for MicroRNA according to claim 1, wherein: the leading-in optical fiber bundle is composed of a plurality of groups of leading-in optical fibers (9), and one ends of the groups of leading-in optical fibers (9) are connected with the sample (2).
3. The high efficiency assay device for MicroRNA according to claim 1, wherein: the number of the leading-in optical fibers (9), the number of the output optical fibers (4), the number of the samples (2) and the number of the focusing lenses (3) are the same.
4. The high efficiency assay device for MicroRNA according to claim 1, wherein: and an optical fiber fixing sleeve (10) is arranged at one end of the leading-in optical fiber bundle close to the tunable laser (1) and one end of the output optical fiber bundle close to the optical filter (5).
5. The high efficiency assay device for MicroRNA according to claim 4, wherein: the optical fiber fixing sleeve (10) is used for fixing the output optical fiber (4) and the lead-in optical fiber (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920399622.4U CN209778861U (en) | 2019-03-27 | 2019-03-27 | Efficient detection device for MicroRNA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920399622.4U CN209778861U (en) | 2019-03-27 | 2019-03-27 | Efficient detection device for MicroRNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209778861U true CN209778861U (en) | 2019-12-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920399622.4U Expired - Fee Related CN209778861U (en) | 2019-03-27 | 2019-03-27 | Efficient detection device for MicroRNA |
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| Country | Link |
|---|---|
| CN (1) | CN209778861U (en) |
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2019
- 2019-03-27 CN CN201920399622.4U patent/CN209778861U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191213 |