CN205280586U - Micro - imaging system of spectrum that fluorescence microscope and fiber optic spectrometer unite - Google Patents
Micro - imaging system of spectrum that fluorescence microscope and fiber optic spectrometer unite Download PDFInfo
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- CN205280586U CN205280586U CN201521030674.2U CN201521030674U CN205280586U CN 205280586 U CN205280586 U CN 205280586U CN 201521030674 U CN201521030674 U CN 201521030674U CN 205280586 U CN205280586 U CN 205280586U
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- optic catheter
- fluorescent microscope
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Abstract
The utility model discloses a micro - imaging system of spectrum that fluorescence microscope and fiber optic spectrometer unite. This system includes fluorescence microscope, fiber optic spectrometer, fibre -optic catheter and one is used for assembling the fiber coupler in this fibre -optic catheter with input light, wherein, fluorescence microscope's C -mount interface through this fiber coupler with fibre -optic catheter one end is connected, this fibre -optic catheter other end with fiber optic spectrometer's signal input part connects. This system has improved the collection ability that fluorescence got into the fibre -optic catheter greatly to collection signal intensity has been improved, simultaneously through addding aperture stop for carry out the election district and arouse under the prerequisite that does not influence sharp luminous intensity, realized surveing the microdomain fluorescence of sample, improved the fluorescence signal and collected intensity.
Description
Technical field
The utility model relates to instrument and equipment field, specifically, relates to the spectrum micro imaging system that a kind of fluorescent microscope is combined with fiber spectrometer.
Background technology
Fluorescence is done again " firefly light ", is a kind of photo-induced cold luminescence phenomenon. When the incident light (normally ultraviolet or X-ray) of certain normal temperature material through certain wavelength irradiates, absorb luminous energy laggard enter excited state, and move back immediately excite and send emergent light (usual wavelength ratio incident light wavelength long, at visible light wave range); And once stopping incident light, luminescence phenomenon also disappears immediately thereupon. The emergent light with this kind of character is just referred to as fluorescence.
Fluorescent microscope take ultraviolet as light source, in order to irradiate tested object, makes it to send fluorescence, then observes shape and the position thereof of object under the microscope. Fluorescent microscope is for studying the distribution and location etc. of the absorption of intracellular matter, transport, chemical substance. Some material in cell, such as chlorophyll etc., by sending out fluorescence after uviolizing; Though separately there being some materials itself can not send out fluorescence, if but with, after fluorescence dye or fluorescent antibody staining, also sending out fluorescence through uviolizing, fluorescent microscope is exactly one of instrument carrying out quantitative and qualitative analysis research to this kind of material.
Fluorescence spectrophotometer, also known as spectrophotofluorometer, is the instrument of a kind of qualitative and quantitative analysis. By the detection of fluorescence spectrophotometer, it is possible to obtain the excitation spectrum of material, emmission spectrum, quantum yield, fluorescence intensity, fluorescence lifetime, Stokes shift, fluorescence polarization and characteristic of depolarizing, and the information of the cancellation aspect of fluorescence. The development of optical fiber technology, makes determinand depart from the restriction in fixed sample pond. Micro fiber spectrometer have internal structure compact, without moving-member, wavelength region width, measuring speed is fast, price is low feature.
At present in microscopic applications, not only there is no and directly observation sample is gone out the method observed image but also can directly provide substance spectra information, but need first to carry out sample observation under the microscope, again sample is transferred to and independent spectrum analyzer carries out the analysis such as composition, content, such asynchronous operation, can cause the change of sample in transfer process and the uncertainty brought. Meanwhile, at cell biological, biophysics and material science, important change often can occur in the miniature scale of Time and place, and simple fluorescence spectrophotometer cannot realize the home position observation in micro-district.
Practical novel content
The purpose of this utility model is to overcome the shortcoming of prior art, it is provided that the spectrum micro imaging system that a kind of fluorescent microscope is combined with fiber spectrometer.
The utility model discloses a kind of spectrum micro imaging system being applied to cell biological, biophysics and material science, feature is building of fluorescent microscope Real Time Image System and optical fiber spectrum imaging system, utilize the C-mount interface of fluorescent microscope to be connected by fiber coupler with fibre-optic catheter, carry out multispectral collection what realize high resolution fluorescence imaging simultaneously. Another feature of the present utility model is the exciting light district at fluorescent microscope, and configuration aperture diaphragm, regulates optical signal, it is possible to realize in the ground imaging of micro-district high resolution. The utility model can realize high speed imaging and catch fluorescent signal in real time, the graphic information caught is carried out multispectral analysis, advantage fluorescent microscope is quick, real-time, original position combines with spectrograph high precision, advantage quantitative, multi-functional, and this system is applicable to the research fields such as cell, gene or protein chip, molecular pathology section, plant section. In addition, this system is to materialogy, and chemistry and physics etc. need the research work of micro-district fluorometric investigation analysis also to have important application prospect.
The technical solution of the utility model is:
The spectrum micro imaging system that a kind of fluorescent microscope is combined with fiber spectrometer, it is characterised in that, comprise fluorescent microscope, fiber spectrometer, fibre-optic catheter and for input light is converged to the fiber coupler in this fibre-optic catheter; Wherein, the C-mount interface of described fluorescent microscope is connected with described fibre-optic catheter one end by this fiber coupler, and this fibre-optic catheter the other end is connected with the signal input terminus of described fiber spectrometer.
Further, the exciting light district configuration one of described fluorescent microscope is for regulating the aperture diaphragm of excitation light irradiation scope.
Further, described fiber coupler comprises a lens combination and a fibre-optic catheter interface; This fibre-optic catheter interface is positioned at described fiber coupler rear end, and this fibre-optic catheter interface is for accessing this fibre-optic catheter; Described lens combination is positioned at the front end of described fiber coupler, for input light converges to the fibre-optic catheter of this fibre-optic catheter interface of access.
Further, described fiber coupler is cylinder shape fiber coupler.
Further, described lens combination comprises along inputting convex lens and the concavees lens that light incident direction is arranged in order.
Further, described fluorescent microscope is inverted fluorescence microscope.
Compared with prior art, the beneficial effects of the utility model:
Native system substantially increases, by fiber coupler, the capacity gauge that fluorescence enters fibre-optic catheter, thus improves collection strength of signal; Simultaneously by setting up aperture diaphragm so that carry out constituency under the prerequisite not affecting excitating light strength and excite, it is achieved that to micro-district fluorescence observation of sample, it is to increase fluorescence signal collection intensity; Native system can at the high resolution fluoroscopic image of fluorescence microscope sample with while it being taken pictures, derive fluorescent signal and send into fiber spectrometer in real time by fibre-optic catheter and carry out fluorescent spectroscopy, be experimental study and other inspection bringing great convenience property of work.
Accompanying drawing explanation
Fig. 1 is one-piece construction figure of the present utility model;
Fig. 2 is side-view of the present utility model;
Fig. 3 is fiber coupler structure iron;
Fig. 4 is aperture diaphragm structure iron;
Fig. 5 is blue light fluorescence nano grain micro-imaging and In situ spectroscopic;
The blue light nanoparticle fluoroscopic image in (a) big visual field; B () constituency excites single nanoparticle fluoroscopic image; (c) blue light fluorescent powder fluorescence nano grain spectrogram;
Fig. 6 is ruddiness fluorescence nano grain micro-imaging and In situ spectroscopic;
(a) big visual field red light Nano grain fluoroscopic image; B () constituency excites single nanoparticle fluoroscopic image; (c) red light fluorescent powder fluorescence nano grain spectrogram;
Wherein, 1-fluorescent microscope, 2-fiber spectrometer, 3-fibre-optic catheter, 4-fiber coupler, 5-aperture diaphragm.
Embodiment
We are by spectroscopic techniques and microscopic imaging fluorescence technology coupling, while utilizing fluorescent microscope and fiber spectrometer that same sample is carried out imaging, original position obtains real time spectrum data, for the research of life science provides a kind of quick, quantitative image analysis tool. Utilize spectroscopic techniques can study the fluorescence kinetics unrelated with the concentration of fluorophore, by distinguishing the micro-point more information about molecular motion, size, residing environment and interaction of spectrum information acquisition in the sample to which. Simultaneously by means of microscopical strength, it is possible to obtain imaging clearly, measure the dynamic process of the local action inside cell and cellularstructure.
As shown in Figure 1, structure of the present utility model: comprise a fluorescent microscope, a micro fiber spectrometer and fibre-optic catheter thereof, a cylinder shape fiber coupler. Configure an aperture diaphragm in the exciting light district of fluorescent microscope, regulate excitation light irradiation scope.
The present embodiment device is by the OLYMPUS inverted fluorescence microscope 1 that a model is IX73, a model is AvaSpec-HS1024x58/122TEC ultra-high sensitive degree type fiber spectrometer 2, and fibre-optic catheter 3, cylinder shape fiber coupler 4, as shown in Figure 3, fiber coupler comprises a lens combination and a fibre-optic catheter interface to its structure; This fibre-optic catheter interface is positioned at fiber coupler rear end, and this fibre-optic catheter interface is for accessing this fibre-optic catheter; Lens combination is positioned at the front end of fiber coupler, for input light converges to the fibre-optic catheter of this fibre-optic catheter interface of access.
As shown in Figure 2, fluorescent microscope tail end is equipped with aperture diaphragm 5; The structure of aperture diaphragm is as shown in Figure 4.
Fig. 5 is blue light fluorescence nano grain micro-imaging and In situ spectroscopic, wherein the blue light nanoparticle fluoroscopic image in (a) big visual field; B () constituency excites single nanoparticle fluoroscopic image; (c) blue light fluorescent powder fluorescence nano grain spectrogram.
Fig. 6 is ruddiness fluorescence nano grain micro-imaging and In situ spectroscopic; Wherein (a) big visual field red light Nano grain fluoroscopic image; B () constituency excites single nanoparticle fluoroscopic image; (c) red light fluorescent powder fluorescence nano grain spectrogram.
Above embodiment is only in order to be further described the utility model, and scope of the present utility model is not by the limitation of illustrated embodiment.
Claims (6)
1. the spectrum micro imaging system that a fluorescent microscope is combined with fiber spectrometer, it is characterised in that, comprise fluorescent microscope, fiber spectrometer, fibre-optic catheter and for input light is converged to the fiber coupler in this fibre-optic catheter; Wherein, the C-mount interface of described fluorescent microscope is connected with described fibre-optic catheter one end by this fiber coupler, and this fibre-optic catheter the other end is connected with the signal input terminus of described fiber spectrometer.
2. the spectrum micro imaging system that fluorescent microscope as claimed in claim 1 is combined with fiber spectrometer, it is characterised in that, the exciting light district configuration one of described fluorescent microscope is for regulating the aperture diaphragm of excitation light irradiation scope.
3. the spectrum micro imaging system that fluorescent microscope as claimed in claim 1 or 2 is combined with fiber spectrometer, it is characterised in that, described fiber coupler comprises a lens combination and a fibre-optic catheter interface; This fibre-optic catheter interface is positioned at described fiber coupler rear end, and this fibre-optic catheter interface is for accessing this fibre-optic catheter; Described lens combination is positioned at the front end of described fiber coupler, for input light converges to the fibre-optic catheter of this fibre-optic catheter interface of access.
4. the spectrum micro imaging system that fluorescent microscope as claimed in claim 3 is combined with fiber spectrometer, it is characterised in that, described fiber coupler is cylinder shape fiber coupler.
5. the spectrum micro imaging system that fluorescent microscope as claimed in claim 3 is combined with fiber spectrometer, it is characterised in that, described lens combination comprises along inputting convex lens and the concavees lens that light incident direction is arranged in order.
6. the spectrum micro imaging system that fluorescent microscope as claimed in claim 1 is combined with fiber spectrometer, it is characterised in that, described fluorescent microscope is inverted fluorescence microscope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521030674.2U CN205280586U (en) | 2015-12-11 | 2015-12-11 | Micro - imaging system of spectrum that fluorescence microscope and fiber optic spectrometer unite |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201521030674.2U CN205280586U (en) | 2015-12-11 | 2015-12-11 | Micro - imaging system of spectrum that fluorescence microscope and fiber optic spectrometer unite |
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| CN205280586U true CN205280586U (en) | 2016-06-01 |
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| CN201521030674.2U Expired - Fee Related CN205280586U (en) | 2015-12-11 | 2015-12-11 | Micro - imaging system of spectrum that fluorescence microscope and fiber optic spectrometer unite |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112859318A (en) * | 2021-01-27 | 2021-05-28 | 锘海生物科学仪器(上海)股份有限公司 | Groove connector for microscope |
| CN113866192A (en) * | 2021-09-27 | 2021-12-31 | 中国科学院高能物理研究所 | Microscopic imaging method and system based on transparent scintillator film |
-
2015
- 2015-12-11 CN CN201521030674.2U patent/CN205280586U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112859318A (en) * | 2021-01-27 | 2021-05-28 | 锘海生物科学仪器(上海)股份有限公司 | Groove connector for microscope |
| CN113866192A (en) * | 2021-09-27 | 2021-12-31 | 中国科学院高能物理研究所 | Microscopic imaging method and system based on transparent scintillator film |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160601 Termination date: 20201211 |