CN206740634U - Device for the detection of Citrus Huanglongbing pathogen field rapid field - Google Patents

Device for the detection of Citrus Huanglongbing pathogen field rapid field Download PDF

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Publication number
CN206740634U
CN206740634U CN201621210745.1U CN201621210745U CN206740634U CN 206740634 U CN206740634 U CN 206740634U CN 201621210745 U CN201621210745 U CN 201621210745U CN 206740634 U CN206740634 U CN 206740634U
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China
Prior art keywords
module
light
field
citrus
detection
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Expired - Fee Related
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CN201621210745.1U
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Chinese (zh)
Inventor
桂家祥
饶敏
郝勇
卢占军
乔宁
刘韬
张岑
温钦华
黄文华
钟延文
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Ganzhou Academy Of Science And Technology For Inspection And Quarantine
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Ganzhou Academy Of Science And Technology For Inspection And Quarantine
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Priority to CN201621210745.1U priority Critical patent/CN206740634U/en
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Abstract

The utility model discloses the device detected for Citrus Huanglongbing pathogen field rapid field, it is characterised in that including:GPS module, detector array, control circuit module, USB2.0 interface modules, beam splitting system, battery module, optical focusing lens module, light source module, sample collection window, touch-screen, ring washer and reflection cap;The utility model realizes the detection identification of the blade of citrus healthy leaves, the infection blade of yellow twig, the blade of nitrogen stress, the blade of magnesium deficiency and zinc-deficiency, detects quick, real-time, lossless, accurate and In vivo detection, testing cost is low, non-environmental-pollution.

Description

Device for the detection of Citrus Huanglongbing pathogen field rapid field
Technical field
Citrus field of planting is the utility model is related to, more particularly to a kind of Citrus Huanglongbing pathogen field based on spectral technique is fast The device of fast Site Detection.
Background technology
The technology both at home and abroad for Citrus Huanglongbing pathogen diagnosis mainly includes at present:Field symptom identification, Indexing by indicator plants, Electron microscopic observation, Serologic detection, molecular Biological Detection and spectral detection etc..Using most common also mainly with field symptom Based on identification, the most typical symptom of yellow twig is the mottled yellow of blade and " red nose fruit ", can be used as the sick field shape mirror Fixed Main Basiss.But the sick symptom is affected by environment larger, in country variant and area, disease it is different infect the stage, Symptom shown by plant has difference.And specificity is not strong, is easily mixed with similar symptoms caused by other reasonses such as nutritional deficiency, longicorns Confuse.Therefore need further to make a definite diagnosis with reference to other identification of means.
Indexing by indicator plants typically uses Citrus sinensis Osbeck or Orlando tangerine shaddock in South Africa, and China mainly uses Ponkan conduct Indicator plant carries out the identification of disease.With indicator plant after pathogen infects, it can show to be reflected with characteristic symptom Not.This method is reliable compared with field diagnosis, but qualification cycle is longer, is unfavorable for quick detection.And electron microscopic observation is suitable due to needing Expensive equipment and do not possess it is specific be seldom used for carrying out yellow twig detection, and Serologic detection is because not yet screening obtains at present Obtaining specific preferably serum causes the development progress of Related product slow.
The most various molecular Biological Detection means for being also limited to PCR-based technology of application at present, such as nido (half Nido) PCR, immunocapture PCR, competitive quantitative PCR, real-time fluorescence quantitative PCR, multiplex PCR and the constant-temperature amplification of ring mediation The detection methods such as technology (LAMP).Wherein with Standard PCR, quantitative PCR and nest-type PRC yellow twig detection in it is the most commonly used, lead to Distinctive DNA fragmentation is crossed in detection Candidatus liberibacter to be identified, such method is contained in Citrus Huanglongbing pathogen detection at present Most sensitive most reliable method.But these technologies are required for greatly a set of specific expensive device, and operate the certain basis of needs And proficiency, it is difficult to carry out the popularization of Fields detection, normally only limit the use of in test in laboratory.
Utility model content
The utility model provides the apparatus and method for the detection of Citrus Huanglongbing pathogen field rapid field, is ensureing blade While complete, citrus nursery stock health, early infection yellow twig, nitrogen stress nutrition, zinc-deficiency can be realized by gathering Spectra of The Leaves Nutrition and effective identification of magnesium deficiency nutrition, the application of Voting Model ensure 100% identification of nursery stock health status and nutrition condition, It is simple to operate, the plantation of scientific guidance citrus, reduce risk.
The utility model is achieved through the following technical solutions above-mentioned purpose:It is quickly existing that one kind is used for Citrus Huanglongbing pathogen field The device of field detection, including:
GPS module, detector array, control circuit module, USB2.0 interface modules, beam splitting system, battery module, optics Condenser lens module, light source module, sample collection window, touch-screen, ring washer and reflection cap;
Wherein, the citrus INFORMATION for infecting yellow twig and gps coordinate are sent to center-side by the GPS module simultaneously; The detector array is used to receive the reflective information reflected through reflection cap from the light that lighting module is launched;The control Circuit module is used to regulate and control circuit and temperature-compensating;The USB2.0 interface modules are used for data transfer and equipment is filled Electricity;The beam splitting system is used to be disperseed the light that light source is sent according to different wavelength;The battery module is the device Power supply is provided;The optical focusing lens module is used to reflected light becoming directional light;The sample collection window is used to place Sample carries out spectra collection;The touch-screen is used for the display and preservation of measurement result and location information;The ring washer is used Enter detection means in suppressing veiling glare;The reflection cap is used to the light for carrying sample message being reflected back detector;
The light source module is included in light path arranges low-power vacuum Halogen lamp LED according to specific angle, covers wavelength model Enclose for:950-1650nm, two lamps are arranged symmetrically in detector both sides.
Preferably, axis at 45 ° angle of the optical axis of every halogen light source along optical path direction and the annular.
Preferably, the spectra collection module includes receiving the optical transform module of sample reflected light and receives the conversion Module transmits the detector array of photon.
Preferably, the output optics conversion module, realizes focusing and the parallel launch of photon, photon information is sent to Light sensor, module are respectively positioned on inside detection means.
The utility model realizes normal citrus nursery stock, early infection yellow twig, nitrogen stress nutrition, zinc-deficiency nutrition and magnesium deficiency battalion Effective identification of the health status such as foster and multinomial nutrition condition, quick and precisely, testing cost is low, non-environmental-pollution for detection.
Brief description of the drawings
Fig. 1 is the schematic device that the utility model is used for the detection of Citrus Huanglongbing pathogen field rapid field;
Fig. 2 is the device lighting module schematic diagram that the utility model is used for the detection of Citrus Huanglongbing pathogen field rapid field;
Fig. 3 is to be illustrated using the utility model device spectra collection annular soft darkening ring and polytetrafluoroethylene (PTFE) reflection cap Figure;
Embodiment
Below in conjunction with the accompanying drawings, the utility model is done for the device of Citrus Huanglongbing pathogen field rapid field detection and retouched in detail State.
As shown in Figure 1:For determining the device of Citrus Huanglongbing pathogen field rapid field detection, including:
GPS module 1:For being sent to center-side simultaneously to the citrus INFORMATION and gps coordinate that infect yellow twig;
Detector array 2 is used to receive the reflective information reflected through reflection cap 12 from the light that lighting module 8 is launched; Control circuit module 3 is used to regulate and control circuit and temperature-compensating;
USB2.0 interface modules 4 are used for data transfer and equipment charge, maximum speed (480Mb/s);Beam splitting system 5 is used for The light that light source is sent is disperseed according to different wavelength.Battery module 6 provides power supply for equipment, and it is small that endurance is more than 6 When;Optical focusing lens module 7, for reflected light to be become into directional light;
As shown in Figure 2:Light source module 8 is used to launch the light of near infrared band scope, using double integrated vacuum tungsten lamps, two Lamp is symmetrically distributed in device top in 45 °;
Sample collection window 9 is used to place sample progress spectra collection, plays a part of protecting light source and fixed light path;
As shown in Figure 3:Touch-screen 10 is used for the display and preservation of measurement result and location information;Ring washer 11 is used to press down Veiling glare processed enters detection means;Polytetrafluoroethylene (PTFE) reflection cap 12 is used to the light for carrying sample message being reflected back detector.
Lighting module 8, spectra collection module and control unit 3 described in the utility model are consuming parts, in order to Ensure the normal work of lighting module 8, spectra collection module and control unit 3, should on the premise of no specified otherwise It is interpreted as lighting module 8, spectra collection module and control unit 3 and is respectively connected with the power supply to match, lighting module 8, spectrum Acquisition module and control unit 3 can use same portable electric source module 6, can also be separately provided electricity according to being actually needed Source, or the transmission by the circuit realiration power supply signal between lighting module 8, spectra collection module and control unit 3. Spatial relation between each consuming parts does not limit strictly, but for the ease of processing and installation as far as possible using integrated The higher circuit arrangement of degree.

Claims (3)

1. the device for the detection of Citrus Huanglongbing pathogen field rapid field, it is characterised in that including:
GPS module, detector array, control circuit module, USB2.0 interface modules, beam splitting system, battery module, optical focus Lens module, light source module, sample collection window, touch-screen, ring washer and reflection cap;
Wherein, the citrus INFORMATION for infecting yellow twig and gps coordinate are sent to center-side by the GPS module simultaneously;It is described Detector array is used to receive the reflective information reflected through reflection cap from the light that lighting module is launched;The control circuit Module is used to regulate and control circuit and temperature-compensating;The USB2.0 interface modules are used for data transfer and equipment charge;Institute Beam splitting system is stated to be used to be disperseed the light that light source is sent according to different wavelength;The battery module provides electricity for the device Source;The optical focusing lens module is used to reflected light becoming directional light;The sample collection window enters for placing sample Row spectra collection;The touch-screen is used for the display and preservation of measurement result and location information;The ring washer is used to suppress Veiling glare enters detection means;The reflection cap is used to the light for carrying sample message being reflected back detector;
The light source module is included in light path arranges low-power vacuum Halogen lamp LED according to specific angle, covers wave-length coverage For:950-1650nm, two lamps are arranged symmetrically in detector both sides.
2. Citrus Huanglongbing pathogen field rapid field detection means as claimed in claim 1, it is characterised in that the spectra collection Module includes receiving the optical transform module of sample reflected light and receives the detector array that the conversion module transmits photon.
3. Citrus Huanglongbing pathogen field rapid field detection means as claimed in claim 2, it is characterised in that the optical transform Module, realizes focusing and the parallel launch of photon, photon information is sent into light sensor, module is respectively positioned in detection means Portion.
CN201621210745.1U 2016-11-10 2016-11-10 Device for the detection of Citrus Huanglongbing pathogen field rapid field Expired - Fee Related CN206740634U (en)

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Application Number Priority Date Filing Date Title
CN201621210745.1U CN206740634U (en) 2016-11-10 2016-11-10 Device for the detection of Citrus Huanglongbing pathogen field rapid field

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201621210745.1U CN206740634U (en) 2016-11-10 2016-11-10 Device for the detection of Citrus Huanglongbing pathogen field rapid field

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106404680A (en) * 2016-11-10 2017-02-15 赣州市检验检疫科学技术研究院 Device and method for field quick on-site detection of citrus huanglongbing

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106404680A (en) * 2016-11-10 2017-02-15 赣州市检验检疫科学技术研究院 Device and method for field quick on-site detection of citrus huanglongbing

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Granted publication date: 20171212

Termination date: 20191110