CN103969188A - Enzyme-labeling measuring instrument detection system based on CCD or CMOS image sensor - Google Patents
Enzyme-labeling measuring instrument detection system based on CCD or CMOS image sensor Download PDFInfo
- Publication number
- CN103969188A CN103969188A CN201310039107.2A CN201310039107A CN103969188A CN 103969188 A CN103969188 A CN 103969188A CN 201310039107 A CN201310039107 A CN 201310039107A CN 103969188 A CN103969188 A CN 103969188A
- Authority
- CN
- China
- Prior art keywords
- optical fiber
- detection system
- light
- microplate reader
- light optical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The present invention relates to an enzyme-labeling measuring instrument detection system, which comprises a light source, an optical filter arranged in front of the light source, import optical fibers, an enzyme label plate, receiving optical fibers, an optical filter arranged in front of a detector, a lens group, a CCD camera or CMOS camera, and a computer. In the system, each cells of the 96-well enzyme label plate is respectively corresponding to the one import optical fibers and the one receiving optical fiber, and the CCD camera or CMOS camera is adopted to image and acquire signals. The system has characteristics of rapid detection, high sensitivity, no requirement of mechanical moving equipment, manufacturing cost reducing and good application value.
Description
Technical field
Patent of the present invention relates to biology and medicine detector device, specifically refers to the microplate reader detection system based on CCD or cmos image sensor.
Technical background
Microplate reader is that enzyme-linked immunosorbent assay instrument (ELISA Reader) is the instrumentation of enzyme linked immunosorbent assay, and it is widely used in biomedical research and clinical diagnosis, is used for detecting and quantitative test immune response.Microplate reader principle of work is exactly that mating plate or monochromator become the light of a branch of specific wavelength to the light wave that sends of illuminator after filtration, entering the sample to be measured in the micropore of ELISA Plate. a light part for this specific wavelength is absorbed by sample, another part sees through sample and is irradiated on photoelectric detector, photoelectric detector is by this sample to be measured is different and strong and weak different light signal converts corresponding electric signal to. and the premenstrual storing of electric signal is large, logarithmic amplification, the signals such as analog to digital conversion are sent into microprocessor after processing and are carried out data processing and calculating, finally show result by display or printer.
Photoelectric detector in existing microplate reader is with photoelectric cell substantially, and photronic advantage is: stable output signal, and noise is little, receiver wavelength range wide (can to more than near infrared 1000nm), shortcoming: sensitivity is low, responds slower.In a general microplate reader, only have and install one, and an ELISA Plate has 96 skies, like this, run through whole ELISA Plate, must want Mechanical Moving mechanism, so just have several shortcomings: 1. complex structure, 2. mechanical mobile device is likely out of order, 3. an ELISA Plate has 96 holes, and existing microplate reader will read respectively 96 ability and all run through, and has extended like this detection time.
Summary of the invention
The object of the invention is to propose a kind of detection sensitivity high, speed is fast, does not need the microplate reader detection system of mechanical mobile device.
The present invention adopts following technical scheme, the detection system of microplate reader is by light source, be placed in the light optical filter before light source, the lead-in light optical fiber of 96,96 hole ELISA Plate, the reception light optical fiber corresponding with each root lead-in light optical fiber, be placed in optical filter, optical lens group, area array CCD camera or CMOS camera before detecting device, computing machine composition.Light source, lead-in light optical fiber, reception light optical fiber are positioned on same axial line, each hole in the ELISA Plate in 96 holes is a corresponding lead-in light optical fiber and reception light optical fiber respectively, the light that can simultaneously send like this specific wavelength arrives in 96 holes of ELISA Plate, also accept the light through 96 holes simultaneously, so just save the mechanical mobile device of existing microplate reader, avoid so on the one hand the out of order possibility of mobile device, also saved detection time on the other hand.
Brief description of the drawings
Fig. 1 is existing microplate reader detection system, once can only detect a hole
Fig. 2 is microplate reader detection system of the present invention, once can detect 96 holes, and lead-in light optical fiber, at the top of ELISA Plate, receives the bottom of light optical fiber in ELISA Plate.
Fig. 3 is microplate reader detection system of the present invention, once can detect 96 holes, and lead-in light optical fiber, in the bottom of ELISA Plate, receives the top of light optical fiber in ELISA Plate.
Fig. 4 is microplate reader detection system light path principle figure of the present invention
Specific implementation method
Existing microplate reader is by detection system, data handling system, and mechanical mobile device forms, the detection system of existing microplate reader as shown in Figure 1, wherein 1-1 has shown in 96 hole ELISA Plate (8 × 12), and 1-2 is the light that light source sends, and 1-3 is the light through ELISA Plate.Existing microplate reader is generally hole of one-time detection, and so just mobile ELISA Plate or detection system repeatedly, to complete the detection in 96 holes.
Microplate reader of the present invention is by detection system, data handling system composition, detection system by light source, be placed in light optical filter before light source, 96 lead-in light optical fiber, 96 hole ELISA Plate, 96 piece optical filter, optical lens group, area array CCD camera or the CMOS camera, the computing machines that receive light optical fiber, be placed in detecting device before corresponding with each root light-conductive optic fibre form, the light that can simultaneously send like this specific wavelength arrives in 96 holes of ELISA Plate, also accept the light through 96 holes simultaneously, so just saved the mechanical mobile device of existing microplate reader.
Shown in Fig. 2, wherein 2-1 has shown in 96 hole ELISA Plate (8 × 12), and 2-2 is many lead-in light optical fiber, and 2-3 is many and receives light optical fiber
Certainly, lead-in light optical fiber can (be shown in Fig. 2) above ELISA Plate, also can below ELISA Plate, (see Fig. 3).
In Fig. 4, each sequence number represents respectively: light source 4-1, be placed in optical filter 4-2 before light source, lead-in light optical fiber 4-3, ELISA Plate 4-4, receive light optical fiber 4-5, be placed in optical filter 4-6, lens combination 4-7, CCD camera or CMOS camera 4-8, computing machine 4-9 before detecting device.
The light that light source 4-1 sends is selected after specific wavelength by the light optical filter 4-2 being placed in before light source, through lead-in light optical fiber 4-3, enter ELISA Plate hole 4-4, see through the light in each hole, through receiving light optical fiber 4-5, be placed in optical filter 4-6 before detecting device and select the light of specific wavelength, lens 4-7 enters CCD camera or CMOS camera 4-8 collection signal after focusing on, and computing machine 4-9 carries out signal processing.
Patent of the present invention has replaced traditional photoelectric cell with CCD or cmos imaging sensor, compared with present conventional microplate reader, detects detection speed fast, highly sensitive, does not need mechanical mobile device, has reduced manufacturing cost, has good using value.
Claims (3)
1. the microplate reader detection system based on ccd image sensor, by light source (4-1), be placed in optical filter (4-2) before light source, lead-in light optical fiber (4-3), ELISA Plate (4-4), receive light optical fiber (4-5), optical filter (4-6), lens combination (4-7), CCD camera or the CMOS camera (4-8), the computing machine (4-9) that are placed in before detecting device form;
It is characterized in that: in the ELISA Plate of described microplate reader detection system, have 96 holes, the corresponding described lead-in light optical fiber of each hole difference wherein and reception light optical fiber, the light source of described light source Halogen lamp LED and lead-in light optical fiber and reception light optical fiber are on same axial line.
2. microplate reader detection system according to claim 1, is characterized in that, described CCD camera or CMOS camera (3-8) are the photoelectric detector of described microplate reader detection system.
3. microplate reader detection system according to claim 1, is characterized in that, the respectively corresponding described lead-in light optical fiber in each hole (3-3) in the ELISA Plate in described 96 holes and reception light optical fiber (3-5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310039107.2A CN103969188A (en) | 2013-02-01 | 2013-02-01 | Enzyme-labeling measuring instrument detection system based on CCD or CMOS image sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310039107.2A CN103969188A (en) | 2013-02-01 | 2013-02-01 | Enzyme-labeling measuring instrument detection system based on CCD or CMOS image sensor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103969188A true CN103969188A (en) | 2014-08-06 |
Family
ID=51238956
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310039107.2A Pending CN103969188A (en) | 2013-02-01 | 2013-02-01 | Enzyme-labeling measuring instrument detection system based on CCD or CMOS image sensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103969188A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198742A (en) * | 2014-08-20 | 2014-12-10 | 曲阜裕隆生物科技有限公司 | Standard machine CCD setting method-enzyme hole marking method |
| CN107102129A (en) * | 2017-05-31 | 2017-08-29 | 上海理工大学 | A kind of ELIASA |
| CN110133245A (en) * | 2019-05-24 | 2019-08-16 | 京东方科技集团股份有限公司 | A kind of detection device and detection method, relevant apparatus of enzyme linked immunological test board |
| WO2019245005A1 (en) * | 2018-06-20 | 2019-12-26 | 株式会社 東芝 | Testing device, production method for said testing device, cell detection method using said testing device, chamber for said testing device, production method for chamber for said testing device, and testing method |
| US11016028B2 (en) | 2017-01-19 | 2021-05-25 | Indevr, Inc. | Parallel imaging system |
| CN113358650A (en) * | 2021-06-01 | 2021-09-07 | 浙江大学 | 96-hole microporous plate reader |
| CN113552337A (en) * | 2021-07-19 | 2021-10-26 | 中山大学 | Full-spectrum high-efficiency microplate reader system |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000058715A2 (en) * | 1999-03-26 | 2000-10-05 | The University Court Of The University Of Glasgow | Assay system |
| JP2004117299A (en) * | 2002-09-27 | 2004-04-15 | Fuji Photo Film Co Ltd | Assay method using enzyme labeled antibody |
| CN1912586A (en) * | 2005-08-09 | 2007-02-14 | 上海枫岭生物技术有限公司 | Detection system of real-time fluorescence quantitative gene extender |
| CN101140224A (en) * | 2006-09-08 | 2008-03-12 | 深圳迈瑞生物医疗电子股份有限公司 | Enzyme mark instrument spectrophotometric detecting optical system, and light diaphragm and optical filter wheel thereof |
| CN101368907A (en) * | 2008-07-14 | 2009-02-18 | 马义才 | Quantum dot marking test strip quantitative detection system based on CMOS image sensor |
| CN201281708Y (en) * | 2008-09-18 | 2009-07-29 | 安图实验仪器(郑州)有限公司 | Enzyme mark instrument optical system |
| CN202166608U (en) * | 2011-06-30 | 2012-03-14 | 深圳市汇松科技发展有限公司 | Detection body group of multi-channel micro-plate reader |
| CN202562843U (en) * | 2012-04-28 | 2012-11-28 | 嘉兴凯实生物科技有限公司 | Microplate reader suitable for open environment |
| CN102854307A (en) * | 2012-09-17 | 2013-01-02 | 北京信息科技大学 | Optical splitting system and optical splitting method for enzyme-linked immunosorbent assay |
-
2013
- 2013-02-01 CN CN201310039107.2A patent/CN103969188A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000058715A2 (en) * | 1999-03-26 | 2000-10-05 | The University Court Of The University Of Glasgow | Assay system |
| JP2004117299A (en) * | 2002-09-27 | 2004-04-15 | Fuji Photo Film Co Ltd | Assay method using enzyme labeled antibody |
| CN1912586A (en) * | 2005-08-09 | 2007-02-14 | 上海枫岭生物技术有限公司 | Detection system of real-time fluorescence quantitative gene extender |
| CN101140224A (en) * | 2006-09-08 | 2008-03-12 | 深圳迈瑞生物医疗电子股份有限公司 | Enzyme mark instrument spectrophotometric detecting optical system, and light diaphragm and optical filter wheel thereof |
| CN101368907A (en) * | 2008-07-14 | 2009-02-18 | 马义才 | Quantum dot marking test strip quantitative detection system based on CMOS image sensor |
| CN201281708Y (en) * | 2008-09-18 | 2009-07-29 | 安图实验仪器(郑州)有限公司 | Enzyme mark instrument optical system |
| CN202166608U (en) * | 2011-06-30 | 2012-03-14 | 深圳市汇松科技发展有限公司 | Detection body group of multi-channel micro-plate reader |
| CN202562843U (en) * | 2012-04-28 | 2012-11-28 | 嘉兴凯实生物科技有限公司 | Microplate reader suitable for open environment |
| CN102854307A (en) * | 2012-09-17 | 2013-01-02 | 北京信息科技大学 | Optical splitting system and optical splitting method for enzyme-linked immunosorbent assay |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198742A (en) * | 2014-08-20 | 2014-12-10 | 曲阜裕隆生物科技有限公司 | Standard machine CCD setting method-enzyme hole marking method |
| US11016028B2 (en) | 2017-01-19 | 2021-05-25 | Indevr, Inc. | Parallel imaging system |
| CN107102129A (en) * | 2017-05-31 | 2017-08-29 | 上海理工大学 | A kind of ELIASA |
| WO2019245005A1 (en) * | 2018-06-20 | 2019-12-26 | 株式会社 東芝 | Testing device, production method for said testing device, cell detection method using said testing device, chamber for said testing device, production method for chamber for said testing device, and testing method |
| JPWO2019245005A1 (en) * | 2018-06-20 | 2021-05-13 | 株式会社東芝 | Testing device, manufacturing method of this testing device, cell detection method using this testing device, cell for this testing device, manufacturing method of cell for this testing device, and testing method |
| JP7030977B2 (en) | 2018-06-20 | 2022-03-07 | 株式会社東芝 | Inspection device and inspection method |
| CN110133245A (en) * | 2019-05-24 | 2019-08-16 | 京东方科技集团股份有限公司 | A kind of detection device and detection method, relevant apparatus of enzyme linked immunological test board |
| CN113358650A (en) * | 2021-06-01 | 2021-09-07 | 浙江大学 | 96-hole microporous plate reader |
| CN113358650B (en) * | 2021-06-01 | 2024-04-16 | 浙江大学 | 96 hole micropore board reader |
| CN113552337A (en) * | 2021-07-19 | 2021-10-26 | 中山大学 | Full-spectrum high-efficiency microplate reader system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103969188A (en) | Enzyme-labeling measuring instrument detection system based on CCD or CMOS image sensor | |
| CN102519908B (en) | Imaging optical microfluid sensing device and method thereof | |
| AU2016301228B2 (en) | Multi excitation-multi emission fluorometer for multiparameter water quality monitoring | |
| CN201852788U (en) | Portable fluorescence detector | |
| CN102305778A (en) | Micro-multispectral fluorescence reception and treatment system | |
| US20200080941A1 (en) | Multi excitation-multi emission fluorometer for multiparameter water quality monitoring | |
| CN202794037U (en) | Device for deducting light source fluctuation of atomic fluorescence photometer | |
| CN101308093A (en) | Parallel multichannel optical detector | |
| CN202057599U (en) | Micro multispectral fluorescent light receiving and processing system | |
| CN202033293U (en) | Chloramphenicol concentration quantitative detection device based on test strip | |
| CN201229294Y (en) | Paralleling multichannel optical detector | |
| CN106404680A (en) | Device and method for field quick on-site detection of citrus huanglongbing | |
| CN1546987A (en) | Minitype biochemical spectrum analyzer | |
| CN203561580U (en) | Optical system for medical examination microplate reader | |
| CN204924927U (en) | Portable full gloss register for easy reference mummification food security rapid analysis appearance | |
| CN203981586U (en) | A kind of bifurcation fiber device for fluoroscopic examination | |
| CN204177810U (en) | Plasma proteins quick analysis system | |
| CN102236039A (en) | Dark current detection method | |
| CN202393698U (en) | Optical system for full-automatic biochemical analyzer | |
| TWI245116B (en) | Detection array luminometer | |
| CN216621465U (en) | Color recognition system | |
| CN201731943U (en) | Wavelength identification device | |
| CN201126424Y (en) | Miniature biochemical analysis instrument | |
| CN201569627U (en) | Special fiber optic device for quantitative PCR detection | |
| CN216082487U (en) | Low-cost fluorescence detection sensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DD01 | Delivery of document by public notice |
Addressee: Suzhou Ying Minji Bioisystech Co., Ltd Document name: Notification of Passing Preliminary Examination of the Application for Invention |
|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140806 |
|
| RJ01 | Rejection of invention patent application after publication |