CN113358650A - 96-hole microporous plate reader - Google Patents
96-hole microporous plate reader Download PDFInfo
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- CN113358650A CN113358650A CN202110610917.3A CN202110610917A CN113358650A CN 113358650 A CN113358650 A CN 113358650A CN 202110610917 A CN202110610917 A CN 202110610917A CN 113358650 A CN113358650 A CN 113358650A
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- optical fiber
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- conducting liquid
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- 239000013307 optical fiber Substances 0.000 claims abstract description 51
- 230000003287 optical effect Effects 0.000 claims abstract description 22
- 238000012545 processing Methods 0.000 claims abstract description 17
- 230000005540 biological transmission Effects 0.000 claims abstract description 12
- 230000003993 interaction Effects 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims description 31
- 238000001514 detection method Methods 0.000 claims description 21
- 238000010438 heat treatment Methods 0.000 claims description 10
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 4
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 4
- 229910052782 aluminium Inorganic materials 0.000 claims description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 4
- 229910052802 copper Inorganic materials 0.000 claims description 4
- 239000010949 copper Substances 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 4
- 229910052709 silver Inorganic materials 0.000 claims description 4
- 239000004332 silver Substances 0.000 claims description 4
- 238000004321 preservation Methods 0.000 abstract description 11
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 238000012423 maintenance Methods 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 3
- 238000007405 data analysis Methods 0.000 abstract 1
- 238000002965 ELISA Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 6
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 6
- 238000012742 biochemical analysis Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 210000002700 urine Anatomy 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 229940109239 creatinine Drugs 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
Abstract
The invention provides a 96-hole micropore plate reader, which comprises an interaction and central processing module, a light source module, a temperature control system, an image transmission system and an image acquisition system, wherein light rays emitted by the light source module according to set parameters pass through a micropore plate arranged on a micropore plate heat preservation frame, are focused on an optical fiber bundle by a condensing lens in 96 through holes, are converged at an optical fiber buncher by an optical fiber bundle adjusting light path, are converted into image signals by a camera through an optical filter selected on an optical filter selector and the image acquisition lens, and are transmitted to the interaction and central processing module for data analysis or sharing, and the temperature control system is designed and can simultaneously emit heat generated by the light source module and maintain a constant temperature environment for the micropore plate. The reader provided by the invention can quickly, accurately and conveniently obtain the concentration of the substance to be measured in the solution; the reaction process of each hole of the microporous plate can be synchronously observed in real time; the cost is low, the operation is simple, the instrument purchase and maintenance cost can be saved, and the popularization and the use in the basic unit are facilitated.
Description
Technical Field
The invention belongs to the field of medical inspection or chemical analysis, and relates to a microplate reader, in particular to a 96-well microplate reader.
Background
With the development of biochemical analysis technologies such as ELISA (enzyme-linked immunosorbent assay) measurement technology and the like, the analysis technology has the characteristics and trends of high flux, high sensitivity, small dose consumption, multiple labeling and the like, so that a microporous plate becomes a standard device in the field of high-flux biochemical analysis, and a detection device represented by an enzyme-labeled instrument also becomes a basic instrument for biochemical analysis. Further development and diversification of biochemical analysis technology also put forward more complex requirements on corresponding detection instruments, and the application of the traditional microplate reader in numerous scenes is limited. In recent years, multifunctional microplate detectors have emerged to meet the growing demand for high-throughput biological analysis techniques. On the premise of sacrificing part of performance indexes, the detector takes a microporous plate with higher density as a carrier, so that the unit area can be compatible with more samples, and the analyzer can carry out more micro-quantitative analysis while saving a large amount of reagent consumption; the capacity of automatic analysis and processing is enhanced, and a full-automatic process integrating sample adding, detection, result transmission, data processing and microplate washing can be realized; on the basis of the original single function, the multifunctional integrated detection can be synchronously realized by matching with a new analysis detection technology. However, the light source of the existing microplate readers and microplate readers is usually a tungsten lamp, and the defects of large overall volume and mass, inconvenience in carrying, high requirement on use environment, low detection speed, high price, high maintenance cost and high difficulty exist, so that the application scene of detection means such as ELISA (enzyme-linked immunosorbent assay) determination technology on a basic level is greatly limited.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a 96-hole microplate reader, which solves the problems that: the problem that the microplate reader and the microplate reader are not portable enough; the detection speed of the microplate reader and the microplate reader is low; the microplate readers and the microplate readers have complicated structures and high manufacturing and maintenance costs.
The purpose of the invention is realized by the following technical scheme: the utility model provides a 96 hole micropore board reading appearance, this reading appearance is including mutual and central processing module, light source module, temperature control system, image transmission system and image acquisition system, mutual and central processing module pass through the wire and link to each other with light source module, temperature control system and image acquisition system simultaneously, the light source radiator of temperature control system is hugged closely to the one side of light source module, and the light of specific colour is sent to the another side, and after light passed the 96 hole micropore boards of placing on temperature control system's micropore board heat preservation frame, it passes through the optic fibre gathering by image transmission system, finally is converted image signal transmission by image acquisition system and gives mutual and central processing module.
Furthermore, 96 groups of luminous sources are distributed on the light source module according to the corresponding positions of 96 holes of the micropore plate, each group of luminous sources comprises a red luminous source, a green luminous source and a blue luminous source, and a light homogenizing plate is covered on the top of the luminous surface of the light source module.
Furthermore, the temperature control system comprises a heat-conducting liquid circulating pump, a light source radiator, a first heat-conducting liquid connecting pipe, a second heat-conducting liquid connecting pipe, a microporous plate heat-preserving frame, a heating coil and a temperature sensor, the heat-conducting liquid circulating pump is connected with a light source radiator which is made of metal with good heat conductivity such as copper, aluminum, silver and the like, the interior of the microporous plate is provided with a heat-conducting liquid flow path, the bottom surface of the microporous plate is tightly attached to the light source module, the first heat-conducting liquid connecting pipe and the second heat-conducting liquid connecting pipe are connected with the light source radiator and the microporous plate heat-preserving frame to form a heat-conducting liquid closed loop, the microporous plate heat preservation frame is made of metal with good heat conductivity such as copper, aluminum, silver and the like, a heat conducting liquid flow path is arranged in the microporous plate heat preservation frame, through holes are reserved at the positions corresponding to the holes on the 96-hole microporous plate, the heating coil is closely attached to the middle upper part of the micropore plate heat preservation frame in a surrounding mode, and the temperature sensor is closely attached to the surface of the micropore plate heat preservation frame.
Furthermore, the image transmission system comprises a condensing lens, an optical fiber fixing seat, an optical fiber bundle and an optical fiber buncher, wherein the condensing lens is arranged in 96 through holes of the micropore plate heat preservation frame, the distance between the condensing lens and the cross section of the optical fiber bundle below the condensing lens is the focal length of the lens, the bottom of each through hole is respectively provided with the optical fiber fixing seat, the optical fiber bundle consists of 96 optical fibers, one end of the optical fiber bundle is coaxially connected with the optical fiber fixing seat, and the other end of the optical fiber bundle is converged into one bundle.
Furthermore, the image acquisition system comprises an optical filter selector, an optical filter, an image acquisition lens and a camera, wherein a plurality of optical filters are uniformly arranged on the optical filter selector in the same diameter, the optical filter can be adjusted to a position coaxial with the light buncher in the image transmission system, the image acquisition lens and the camera are sequentially coaxially arranged with the optical fiber buncher, the optical filter is arranged on the optical fiber buncher, the camera is arranged above the optical fiber buncher, and the distance between the optical fiber buncher and the camera is the focal length of the image acquisition lens.
Furthermore, the interaction and central processing module is provided with a touch screen, can modulate the color, brightness and color temperature of the light source module and the temperature kept by the temperature control system, can extract RGB parameters of the image, calculates a standard curve according to the parameter values of the specific positions where the standard products are placed, substitutes the parameter values of other positions into the standard curve to compare to obtain a detection result, and presents the image detection result to a user or shares the image detection result with other intelligent terminals.
The principle of the invention is as follows: some biochemical analysis techniques such as ELISA measurement technique are to quantitatively analyze the absorbance of a reaction substrate in a microplate to obtain a detection result. The absorbance can be reflected by the depth of the color of the image in the image technology, and the accuracy and the sensitivity of the image to the absorbance reflection can be improved by the modulation of parameters such as the color of background light, the color temperature and the like and the use of a filter. The principle of the invention is that after the biochemical detection steps such as ELISA and the like are completed, a micropore plate with reaction substrates is placed at a corresponding position of a micropore plate reader, and light emitted by a light source according to modulated parameters is transmitted through the reaction substrates, converged by a lens and then projected onto the cross section of an optical fiber. The optical fiber can change the optical path of the optical fiber without damage, and the imaging area is reduced by bundling the optical fiber. And finally, the camera acquires an image after the optical fibers are gathered, and analyzes information such as the concentration of a specific substance in the corresponding reaction substrate according to the color condition of the corresponding position in the image.
Compared with the prior art, the invention has the following advantages and effects:
(1) compared with an enzyme-labeling instrument, the invention has higher sensitivity and shorter analysis time, and has a more intuitive and flexible display mode through corresponding software, thereby realizing the real-time issuing, long-term storage and network sharing of a diagnosis report.
(2) The POCT detection device has the advantages of small volume, light weight, better portability and simple operation, better meets the requirements of POCT, can be more flexibly adapted to various environments, can realize field and bedside detection, and is more suitable for primary medical units and household detection to deal with emergent public health events.
(3) The invention has simple structure and lower power consumption, improves the stability and reliability of the instrument, and simultaneously reduces the purchase and maintenance cost of the instrument.
(4) The invention has wide application scene and can be used for various detection means and items such as ELISA, spectrophotometry and the like.
Drawings
Fig. 1 is a schematic structural diagram of the present invention, wherein: 1-an interaction and central processing module, 2-a camera, 3-an image acquisition lens, 4-an optical filter, 5-an optical filter selector, 6-an optical fiber buncher, 7-an optical fiber bundle, 8-an optical fiber fixing seat, 9-a temperature sensor, 10-a heating coil, 11-a microporous plate heat preservation frame, 12-a condensing lens, 13-a kit, 14-a first heat-conducting liquid connecting pipe, 15-a second heat-conducting liquid connecting pipe, 16-a light source module, 17-a light source radiator and 18-a heat-conducting liquid circulating pump;
fig. 2 is a schematic structural diagram of a light source module of the present invention, wherein: 19-even light plate, 20-red light source, 21-green light source and 22-blue light source.
Detailed Description
The embodiments of the present invention will be described in detail with reference to the accompanying drawings; the present examples are illustrative and not restrictive, and the scope of the invention is not limited thereto.
Example 1
As shown in fig. 1 and 2, the invention provides a 96-well microplate reader, which comprises an interaction and central processing module, a light source module, a temperature control system, an image transmission system and an image acquisition system, wherein the interaction and central processing module 1 is provided with a large touch screen, and is connected with the light source module 16, a temperature sensor 9 and a heating coil 10 of the temperature control system and a camera 2 of the image acquisition system through leads, so that a feedback result of the temperature sensor 9 can be obtained, parameters of the light source module 16 and the heating coil 10 can be modulated, and meanwhile, the collected image result can be processed or shared with other intelligent terminals; the light source module 16 is distributed with 96 groups of luminous sources according to the corresponding positions of 96 holes of the microporous plate, each group of luminous sources comprises a red luminous source 20, a green luminous source 21 and a blue luminous source 22, the top of the luminous surface is covered with a light homogenizing plate, one surface can emit light rays with specific colors according to parameters set by the interactive and central processing module, and the other surface is tightly attached to the light source radiator 17 of the temperature control system to radiate heat generated by work and maintain a stable working state; the temperature control system comprises a heat-conducting liquid circulating pump 18, a light source radiator 17, a first heat-conducting liquid connecting pipe 14, a second heat-conducting liquid connecting pipe 15, a microporous plate heat-insulating frame 11, a heating coil 10 and a temperature sensor 9, the heat-conducting liquid circulating pump 18 is connected with the light source radiator 17 to drive the heat-conducting liquid to circulate in a closed loop formed by the flow path in the light source radiator 17, the first heat-conducting liquid connecting pipe 14, the flow path in the micropore plate heat-insulating frame 11 and the second heat-conducting liquid connecting pipe 15, so that heat generated by the light source module 16 is transferred to the micropore plate heat-insulating frame 11 for heat insulation of the micropore plate 13, the micropore plate 13 placed on the micropore plate heat-insulating frame 11 can maintain a constant temperature environment, through holes are reserved at corresponding positions of all the holes, the heating coil 10 is closely attached to the middle upper part of the micropore plate heat-insulating frame 11 in a surrounding manner for preheating and rapidly heating when the temperature is insufficient, and the temperature sensor 9 is closely attached to the surface of the micropore plate heat-insulating frame 11 for feeding back the currently maintained temperature; the image transmission system comprises a condensing lens 12, an optical fiber fixing seat 8, an optical fiber bundle 7 and an optical fiber buncher 6, light rays emitted by a light source module 16 pass through a micropore plate 13 arranged on a micropore plate heat preservation frame 11, and are focused to the optical fiber bundle 7 consisting of 96 optical fibers on the optical fiber fixing seat 8 arranged at the bottom of the micropore plate heat preservation frame 11 by the condensing lens 12 in 96 through holes of the micropore plate heat preservation frame 11, and light paths are adjusted by the optical fiber bundle 7 to be converged at the optical fiber buncher 6; the image acquisition system comprises an optical filter selector 5, an optical filter 4, an image acquisition lens 3 and a camera 2, wherein an image formed by converging an optical fiber 7 on an optical fiber buncher 6 passes through the optical filter 4 selected on the optical filter selector 4, is adjusted in an imaging range by the image acquisition lens 3, is finally captured by the camera 2 and is converted into an image signal, and the image signal is transmitted to the interaction and central processing module 1.
Example 2
Creatinine in urine is detected based on a 96-well microplate reader shown in fig. 1, and the steps are as follows:
(1) the temperature of the temperature control system is set to be 37 ℃ by the 96-well microplate reader, the optical filter is selected to be 510nm, the light source module is set to be green light, preheating is started, and background light calibration is completed.
(2) The plate was prepared by dispensing creatinine solutions of 150. mu.L, 2.5. mu.M, 5. mu.M, 10. mu.M, 12.5. mu.M, 20. mu.M, 25. mu.M, 50. mu.M and 100. mu.M in 9 selected wells, respectively.
(3) Diluting 50 mul of urine sample to be tested by 200 times, and then placing 150 mul of the urine sample in the 10 th hole of the microporous plate.
(4) 50 μ L of 25mM picric acid solution followed by 50 μ L of 0.75M sodium hydroxide solution were added to each well.
(5) The microplate was placed in a reader to allow the mixed solution to react at 37 ℃ for 10 minutes.
(6) And (4) recording the image at the moment by using a reader, analyzing the image of each hole to obtain data of each hole, generating a standard curve by using the data of the first nine holes, and substituting the standard curve into the data of the 10 th hole to finally obtain the concentration of creatinine in the urine.
The invention simplifies the operation process of detection and improves the convenience of using the instrument; meanwhile, the detection speed is improved, and the state of each hole of the microporous plate can be synchronously observed in real time; and a large amount of inspection data can be stored and can be retrospectively analyzed or shared to other intelligent terminals.
The above-described embodiments are intended to illustrate rather than to limit the invention, and any modifications and variations of the present invention are within the spirit of the invention and the scope of the appended claims.
Claims (6)
1. The utility model provides a 96 hole micropore board reader which characterized in that: the reader comprises an interaction and central processing module, a light source module, a temperature control system, an image transmission system and an image acquisition system, wherein the interaction and central processing module is simultaneously connected with the light source module, the temperature control system and the image acquisition system through leads, one surface of the light source module is tightly attached to a light source radiator of the temperature control system, the other surface of the light source module emits light with a specific color, and after the light passes through a 96-hole microporous plate arranged on a microporous plate heat-insulating frame of the temperature control system, the light is gathered by the image transmission system through optical fibers and finally converted into image signals by the image acquisition system and transmitted to the interaction and central processing module.
2. The 96-well microplate reader of claim 1, wherein: the light source module is distributed with 96 groups of luminous sources according to the corresponding positions of 96 holes of the micropore plate, each group of luminous source comprises a red luminous source, a green luminous source and a blue luminous source, and the top of the luminous surface of the light source module is covered with a light homogenizing plate.
3. The 96-well microplate reader of claim 1, wherein: the temperature control system comprises a heat-conducting liquid circulating pump, a light source radiator, a first heat-conducting liquid connecting pipe, a second heat-conducting liquid connecting pipe, a microporous plate heat-insulating frame, a heating coil and a temperature sensor, wherein the heat-conducting liquid circulating pump is connected with the light source radiator, the light source radiator is made of metal with good heat conductivity such as copper, aluminum and silver, a heat-conducting liquid flow path is arranged in the heat-conducting liquid circulating pump, a light source module is tightly attached to the bottom surface of the heat-conducting liquid circulating pump, the first heat-conducting liquid connecting pipe and the second heat-conducting liquid connecting pipe are connected with the light source radiator and the microporous plate heat-insulating frame to form a heat-conducting liquid closed loop, the microporous plate heat-insulating frame is made of metal with good heat conductivity such as copper, aluminum and silver, a heat-conducting liquid flow path is arranged in the heat-conducting liquid circulating pump, through holes are reserved in positions corresponding to holes in 96-hole microporous plates, the heating coil is tightly attached to the middle upper portion of the microporous plate heat-insulating frame, and the temperature sensor is tightly attached to the surface of the microporous plate heat-insulating frame.
4. The 96-well microplate reader of claim 1, wherein: the image transmission system comprises a condensing lens, optical fiber fixing seats, optical fiber bundles and an optical fiber buncher, wherein the condensing lens is arranged in 96 through holes of the micropore plate heat-insulating frame, the distance between the condensing lens and the cross section of the optical fiber bundle below the condensing lens is the focal length of the lens, the optical fiber fixing seats are respectively arranged at the bottoms of the through holes, the optical fiber bundles are composed of 96 optical fibers, one end of each optical fiber bundle is coaxially connected with the optical fiber fixing seats, and the other end of each optical fiber bundle is converged into one bundle in the optical fiber buncher.
5. The 96-well microplate reader of claim 1, wherein: the image acquisition system comprises an optical filter selector, optical filters, an image acquisition lens and a camera, wherein a plurality of optical filters are uniformly arranged on the optical filter selector in the same diameter, the optical filters can be adjusted to the position coaxial with the light buncher in the image transmission system, the image acquisition lens and the camera are sequentially coaxially arranged with the optical fiber buncher, the optical filters are arranged on the optical fiber buncher, the camera is arranged above the optical fiber buncher, and the distance between the optical fiber buncher and the camera is the focal length of the image acquisition lens.
6. The 96-well microplate reader of claim 1, wherein: the interaction and central processing module is provided with a touch screen and can modulate the color, brightness and color temperature of the light source module and the temperature kept by the temperature control system; the RGB parameters of the image can be extracted, a standard curve is calculated according to the parameter values of the specific positions where the standard products are placed, the parameter values of other positions are substituted into the standard curve to be compared to obtain a detection result, and the image detection result is presented to a user or shared to other intelligent terminals.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114088706A (en) * | 2021-10-28 | 2022-02-25 | 中央民族大学 | Biochemical detection image acquisition system and image acquisition method |
| CN114088706B (en) * | 2021-10-28 | 2024-04-26 | 中央民族大学 | Biochemical detection image acquisition system and image acquisition method |
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