CN114088706A

CN114088706A - Biochemical detection image acquisition system and image acquisition method

Info

Publication number: CN114088706A
Application number: CN202111266001.7A
Authority: CN
Inventors: 耿照新; 苗焱蕊
Original assignee: Minzu University of China
Current assignee: Minzu University of China
Priority date: 2021-10-28
Filing date: 2021-10-28
Publication date: 2022-02-25
Anticipated expiration: 2041-10-28
Also published as: CN114088706B

Abstract

The invention provides a biochemical detection image acquisition system and an image acquisition method, wherein the biochemical detection image acquisition system comprises a camera bellows, a light source, a micro-lens array and a plurality of optical fibers, wherein a first chamber and a second chamber are arranged in the camera bellows at intervals along the direction vertical to the height, the first chamber is used for accommodating a perforated plate with a plurality of perforated wells arranged in an array, a window is arranged at the position, facing the second chamber, of the top surface of the camera bellows, the window penetrates into the second chamber, and the top of the camera bellows is used for mounting portable electronic equipment with a camera; the light source is arranged at the top in the first cavity; one end of each optical fiber in the optical fibers is fixed at the bottom in the first cavity to form an optical fiber input array, and the other end of each optical fiber is fixed at the bottom in the second cavity to form an optical fiber output array; the micro lens array is arranged above the optical fiber input array. The biochemical detection image acquisition system can acquire quantitative detection images with large flux and high precision, and has the advantages of simple structure and portability.

Description

Biochemical detection image acquisition system and image acquisition method

Technical Field

The invention relates to the technical field of biochemical detection, in particular to a biochemical detection image acquisition system and an image acquisition method.

Background

Colorimetric methods and fluorescence methods are widely used in the fields of food allergen detection, urine analysis, blood analysis, water quality monitoring, and the like. The traditional colorimetric method is used for analyzing the content of a detected object through naked eyes or a spectrophotometer, and is simple and convenient to operate and intuitive in experimental effect. The main principle of fluorescence detection as a special colorimetric method is that molecules of certain substances are in an excited state after being irradiated by light with a specific wavelength, and specific fluorescence emitted by the molecules undergoing collision and excitation processes can be qualitatively or quantitatively analyzed. The traditional spectrometers used for colorimetric and fluorescence methods are difficult to be widely used in poor or remote areas due to their disadvantages of large size, high price, etc.

Modern smart phones are generally equipped with a fast multi-core processor, a touch screen, a large-capacity battery, and various elements (such as an optical imaging system camera, an accelerometer, a hygrometer, a fingerprint scanner, a heart rate sensor, and the like) that can be used for detection and measurement, and have been upgraded from the original portable communication tools to microcomputers, and have the characteristics of convenience in carrying, low cost, simplicity and convenience in operation, strong computing power, and the like. The intelligent mobile phone is combined with various laboratory-level detection means, so that a powerful tool can be provided for scientific experiments and clinical instant diagnosis. For example, the ability of a smartphone to capture color may be combined with colorimetric and fluorometric methods. A smart phone equipped with a CMOS (Complementary Metal Oxide Semiconductor) sensor, which can capture an optical signal and convert it into an RGB (red, green, blue) color system value range (0-255) by a built-in algorithm; through the calibration curve and the specific data processing method, the RGB intensity value corresponds to the content of the measured object, thereby realizing qualitative or quantitative calibration. The intelligent mobile phone is combined with well-designed hardware equipment, so that the detection result level of the spectrometer can be reached, the intelligent mobile phone is convenient to carry, and the high-precision detection of a sample on site is realized. At present, smart phones have been widely used in food, environmental, agricultural and medical testing.

The biochemical detection system adopts a 96-pore plate as an experimental instrument, has the characteristics of high flux and extremely high detection efficiency, and can perform detection experiments on multiple-flux small samples. When the existing colorimetric or fluorescent method is combined with a smart phone for use, the smart phone is usually used for directly shooting a 96-well plate, and then image processing and qualitative and quantitative calculation are carried out on the obtained picture. However, the cross-sectional area of the 96-hole plate is relatively large, so that the 96-hole plate can be completely placed in the view-finding frame only by a large object distance when the 96-hole plate is shot by using a smart phone, the requirement on the longitudinal size is increased, and the portability of equipment is affected; when the smart phone shoots the 96-pore plate by using the wide-angle lens, the edge of the shot image is easy to distort in a limited size due to a large imaging area, so that the detection sensitivity is not high, and accurate quantitative detection is difficult to perform; in addition, the smooth surface of the 96-well plate reflects light, which is very demanding for the dark room of the sample and the image pre-processing process. Therefore, a portable high-precision biochemical detection image acquisition system and method capable of reducing the image preprocessing requirement are needed.

Disclosure of Invention

The invention aims to provide a biochemical detection image acquisition system and an image acquisition method, which are used for solving the technical problems of large volume, poor portability, low detection sensitivity and high requirement on an image preprocessing process of the conventional colorimetric array image acquisition equipment.

In order to solve the above technical problem, the present invention provides a biochemical detection image acquisition system, including:

the camera comprises a camera box, a camera body and a camera, wherein a first cavity and a second cavity are arranged in the camera box at intervals along the direction vertical to the height, the first cavity is used for accommodating a porous plate with a plurality of wells arranged in an array, a window is arranged at the position, right opposite to the second cavity, of the top surface of the camera box, the window penetrates into the second cavity, and the top of the camera box is used for mounting portable electronic equipment with a camera;

a light source disposed at a top portion within the first chamber;

a plurality of optical fibers, one end of each of the optical fibers in the plurality of optical fibers being fixed to the bottom of the first chamber to form an optical fiber input array, and the arrangement of the end faces of the optical fibers in the optical fiber input array being the same as the arrangement of the wells in the porous plate, and the other end of each of the optical fibers in the plurality of optical fibers being fixed to the bottom of the second chamber to form an optical fiber output array;

the micro lens array is arranged in the first cavity and positioned above the optical fiber input array, and the centers of the micro lenses are aligned with the centers of the end faces of the optical fibers of the optical fiber input array one by one.

According to the biochemical detection image acquisition system provided by the invention, the cross-sectional area of the optical fiber output array is 1/100 of the cross-sectional area of the porous plate.

According to the biochemical detection image acquisition system provided by the invention, the biochemical detection image acquisition system further comprises a first optical insertion sheet, and the first optical insertion sheet is detachably arranged in a light path between the light source and the porous plate.

According to the biochemical detection image acquisition system provided by the invention, the biochemical detection image acquisition system further comprises a second optical insertion sheet, and the second optical insertion sheet is detachably arranged in a light path between the porous plate and the micro lens array.

According to the biochemical detection image acquisition system provided by the invention, the biochemical detection image acquisition system further comprises an optical fiber array panel, the optical fiber array panel comprises an incident array and an emergent array, the incident array is provided with a plurality of incident holes arranged in an array, the arrangement of the incident holes is the same as that of the hole wells on the perforated plate, and the emergent array is provided with a plurality of emergent holes arranged in an array; the optical fiber array panel is arranged at the bottoms of the first cavity and the second cavity, the incident array is positioned in the first cavity, the emergent array is positioned in the second cavity, and the centers of the incident holes are aligned with the centers of the micro lenses one by one; one end of each optical fiber in the optical fibers is fixedly connected to the incident hole, and the other end of each optical fiber is fixedly connected to the emergent hole;

an annular supporting part is arranged on the optical fiber array panel and surrounds the incident array, and the annular supporting part is used for supporting the micro lens array.

According to the biochemical detection image acquisition system provided by the invention, the optical fiber array panel is also provided with an annular clamping groove around the annular supporting part, and the annular clamping groove is used for being matched and clamped with the bottom of the porous plate.

According to the biochemical detection image acquisition system provided by the invention, at least two opposite inner side walls which are vertical to the top surface in the first chamber are provided with first supporting parts, and the light source is supported on the first supporting parts and is detachably connected with the first supporting parts.

According to the biochemical detection image acquisition system provided by the invention, the light source further comprises a power supply interface, an interface opening is further formed in the side wall of the camera bellows, which is adjacent to the first supporting part, and the power supply interface is embedded in the interface opening and is exposed from the outer side wall of the camera bellows.

According to the biochemical detection image acquisition system provided by the invention, at least two opposite inner side walls perpendicular to the top surface in the first chamber are also provided with second supporting parts, and the first optical insert is supported on the second supporting parts and is detachably connected with the second supporting parts.

The invention also provides a biochemical detection image acquisition method, which comprises the following steps:

determining light source parameters of a biochemical detection image acquisition system according to the characteristics of the detected object and the biochemical detection experiment requirements;

determining parameters of a first optical insert and a second optical insert of a biochemical detection image acquisition system according to the characteristics of a detected object and the requirements of biochemical detection experiments;

preparing a sample of a measured object, and storing the sample of the measured object in a plurality of well wells of a multi-well plate;

confirming that the light source is in a closed state, sending the porous plate into a first chamber of a biochemical detection image acquisition system, and confirming that a dark box of the biochemical detection image acquisition system is in a closed state;

installing the portable electronic equipment with the camera on the top of the camera bellows, enabling the camera to be opposite to the window, and opening the camera;

and turning on a light source, carrying out focusing operation on the camera on the portable electronic equipment, and carrying out photographing operation after the camera is successfully focused to obtain a biochemical detection image.

According to the biochemical detection image acquisition system and the image acquisition method provided by the invention, the shot view field can be obviously reduced by arranging the optical fiber array, the shooting and imaging of portable electronic equipment are facilitated, the limitation of the focal distance is reduced, the volume of an optical structure is reduced, the volume of the biochemical detection image acquisition system is further reduced, and the portability is improved; through set up the microlens array above the optical fiber input array, the microlens can assemble the input terminal surface of optic fibre with the light that the well bottom jetted out in, promote the receipts light efficiency of optic fibre greatly, the intensity of the light in the optic fibre is advanced in the reinforcing coupling, improve the coupling efficiency of light, promote the light intensity of optic fibre output light beam, improve the image acquisition precision, thereby promote detectivity and accuracy, and then improve quantitative measurement precision, can carry out the mass flux, the quantitative determination image acquisition of high accuracy, and simple structure, and convenient to carry, and is with low costs, can satisfy the laboratory, hospital etc. is to the needs that the biochemistry detected, can be in medical diagnosis, food safety, extensive popularization and application in fields such as environment supervision.

Drawings

In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings needed for the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and those skilled in the art can also obtain other drawings according to the drawings without creative efforts.

FIG. 1 is a schematic perspective view of a biochemical detection image acquisition system according to an embodiment of the present invention;

FIG. 2 is an exploded view of a biochemical detection image acquisition system according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of the positions of a light source, a first optical insert, a multi-well plate, a second optical insert, a micro-lens array, a fiber array panel, an optical fiber, a double cemented lens, and a portable electronic device according to an embodiment of the invention;

FIG. 4 is a schematic optical path diagram of a biochemical detection image acquisition system according to an embodiment of the present invention;

FIG. 5 is a schematic structural diagram of a microlens array according to an embodiment of the present invention;

FIG. 6 is a schematic diagram showing a comparison of the areas of a multi-well plate and an exit array according to an embodiment of the present invention;

FIG. 7 is a schematic diagram of a light source according to an embodiment of the present invention;

FIG. 8 is a schematic diagram of a fiber array panel and fiber assembly according to an embodiment of the present invention;

FIG. 9 is a diagram illustrating an open state of a magnetic side door of a biochemical detection image acquisition system according to an embodiment of the present invention;

FIG. 10 is a flowchart of a biochemical detection image acquiring method according to an embodiment of the present invention.

In the figure:

1. a dark box; 11. a first chamber; 111. a first support section; 112. a second support portion; 113. a magnetic member; 12. a second chamber; 13. a third chamber; 14. a box body; 141. an upper box body; 142. a lower box body; 143. a top cover; 15. magnetic suction side door; 16. a strip-shaped opening; 17. magnetically attracting the baffle; 18. a third chamber opening; 19. an interface opening;

2. a light source; 21. a light emitting element; 22. a substrate; 23. a protection resistor; 24. a power supply interface;

3. a microlens array; 31. a microlens; 32. a plate body;

4. an optical fiber;

5. a perforated plate; 51. a bore well;

6. a double cemented lens; 60. a lens barrel;

71. a first optical insert; 72. a second optical insert;

8. a fiber array panel; 81. an incident array; 82. an exit array; 83. entering a perforation hole; 84. an exit aperture; 85. an annular support portion; 86. an annular limiting part; 87. a groove; 88. an annular neck; 89. a yielding groove;

9. a mounting member; 91. mounting grooves; 92. a camera opening; 93. an arc-shaped slot;

200. a smart phone; 201. a camera is provided.

Detailed Description

The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

In the description of the embodiments of the present invention, it should be noted that the terms "first" and "second" are used for the sake of clarity in describing the numbering of the components of the product and do not represent any substantial difference, unless explicitly stated or limited otherwise. "upper", "lower", "left", "right", and the like are used only to indicate relative positional relationships, and when the absolute position of the object to be described is changed, the relative positional relationships may also be changed accordingly. Specific meanings of the above terms in the embodiments of the present invention can be understood by those of ordinary skill in the art according to specific situations.

It is to be understood that, unless otherwise expressly specified or limited, the term "coupled" is used broadly, and may, for example, refer to directly coupled devices or indirectly coupled devices through intervening media. Specific meanings of the above terms in the embodiments of the present invention can be understood in specific cases by those of ordinary skill in the art.

As shown in fig. 1 and fig. 2, an image capturing system for biochemical detection according to an embodiment of the present invention includes a dark box 1, a light source 2, a microlens array 3, and a plurality of optical fibers 4.

The camera bellows comprises a camera bellows 1, a camera bellows 5, a camera bellows 51, a camera bellows, a camera lens and a camera lens, wherein a first chamber 11 and a second chamber 12 are arranged in the camera lens 1 at intervals along the direction perpendicular to the height, the first chamber 11 is a detection chamber of a detected object sample, the first chamber 11 is used for accommodating a porous plate 5 with a plurality of porous wells 51 arranged in an array, the porous plate 5 is used for accommodating the carrier for accommodating the sample of the detected object, one porous plate 51 is used for accommodating one sample, one sample for accommodating the one sample, the porous plate 51, and can realize the contrast experiment of the porous plate for one sample for realizing the large flux detection; the second chamber 12 is not communicated with the first chamber 11 along the direction vertical to the height of the dark box 1 so as to avoid the mutual influence of the light rays in the first chamber 11 and the second chamber 12; a window (not shown) is arranged at the position, opposite to the second chamber 12, of the top surface of the dark box 1, and the window penetrates into the second chamber 12; the top of the camera chamber 1 can detachably mount a portable electronic device having a camera.

The light source 2 is disposed at the top of the first chamber 11 for emitting illumination light, and when the porous plate 5 is disposed in the first chamber 11, the light source 2 is located above the porous plate 5.

The number of the optical fibers 4 is the same as that of the hole wells 51 on the porous plate 5, one end of each optical fiber 4 in the optical fibers 4 is fixed at the bottom in the first chamber 11, the end faces of the optical fibers 4 are arranged in a planar array at the bottom in the first chamber 11 to form an optical fiber input array at the bottom in the first chamber 11, the arrangement of the optical fiber input array is the same as that of the hole wells 51 array, and when the porous plate 5 is received in the first chamber 11 and the hole wells 51 array are aligned with the optical fiber input array, the centers of the hole wells 51 are aligned with the centers of the end faces of the optical fibers 4 in the optical fiber input array one by one; meanwhile, the other end of each optical fiber 4 in the plurality of optical fibers 4 is fixed at the bottom in the second chamber 12, and the other end faces of the plurality of optical fibers 4 are also arranged in a planar array at the bottom in the second chamber 12, so as to form an optical fiber output array at the bottom in the second chamber 12.

The micro lens array 3 is provided with a plurality of micro lenses 31 arranged in an array, the number of the micro lenses 31 is the same as that of the optical fibers 4, the arrangement of the micro lens array 3 is the same as that of the optical fiber input array, the micro lens array 3 is arranged at the bottom in the first chamber 11 and is positioned above the optical fiber input array, the micro lens array 3 is aligned with the optical fiber input array, and the centers of the micro lenses 31 are aligned with the centers of the end faces of the optical fibers 4 of the optical fiber input array one by one, so that each micro lens 31 is associated with the end face of one optical fiber 4; when the multi-well plate 5 is received in the first chamber 11 and the array of wells 51 is aligned with the input array of optical fibers, the microlens array 3 is positioned below the multi-well plate 5, the array of wells 51 is also aligned with the microlens array 3, and the centers of the plurality of wells 51 are aligned one-to-one with the centers of the plurality of microlenses 31 such that each well 51 is associated with one microlens 31.

Thus, as shown in fig. 3, in the first chamber 11, the light source 2, the well 51 of the perforated plate 5, the microlens 31 of the microlens array 3, and the end face of the optical fiber 4 of the optical fiber input array are arranged in vertical center alignment; within the second chamber 12, the fiber output array is arranged in vertical, centrally aligned relation with the camera 201 of the portable electronic device. One end of the optical fiber 4 is positioned at the bottom of the well 51 of the porous plate 5 to obtain optical information at the bottom of the well 51, and then the optical information (including fluorescence, color development and color change) is transmitted to the emergent end of the optical fiber 4; the optical fiber 4 is a transmission tool of extremely fine light, the emergent end face of the optical fiber 4 can be gathered to form a neat optical fiber output array, the photographed view field of the optical fiber output array is obviously reduced compared with the plate surface perpendicular to the well 51 of the perforated plate 5, the portable electronic equipment is convenient to photograph and image, the limitation of the focusing distance is reduced, the volume of an optical structure is reduced, the volume of a biochemical detection image acquisition system is further reduced, and the portability is improved.

Fig. 4 is a schematic diagram of an optical path of a biochemical detection image acquisition system according to an embodiment of the present invention during operation; the solid arrows in fig. 4 indicate the propagation direction of the light rays. When the portable electronic device starts to work, the camera 201 of the portable electronic device is opposite to the window; after light emitted by the light source 2 penetrates through a hole well 51 of a perforated plate 5 filled with a sample, a light beam is coupled into an optical fiber 4 by a micro lens 31 of the micro lens array 3 and positioned in the end face of the optical fiber input array, and an optical signal is transmitted to the end face of the optical fiber output array by the optical fiber 4 to form a luminous optical fiber output array; the emergent light beams of the optical fiber output array are focused on the CMOS sensor by the camera 201 of the portable electronic equipment to form image information, so that the image acquisition is realized.

The biochemical detection image acquisition system is used for being combined with portable electronic equipment with a camera 201 to meet the requirement of multi-flux biochemical detection on a detected object; by arranging the optical fiber 4 array, the shot view field can be obviously reduced, the shooting and imaging of portable electronic equipment are facilitated, the limitation on the focal distance is reduced, the volume of an optical structure is reduced, the volume of a biochemical detection image acquisition system is further reduced, and the portability is improved; by arranging the micro lens array 3 above the optical fiber input array, the micro lens 31 can completely converge the light emitted from the bottom of the hole well 51 into the input end face of the optical fiber 4, so that the light receiving efficiency of the optical fiber 4 is greatly improved, the intensity of the light coupled into the optical fiber 4 is enhanced, the coupling efficiency of the light is improved, the light intensity of the light beam output by the optical fiber 4 is improved, the image acquisition precision is improved, the detection sensitivity and accuracy are improved, and the quantitative measurement precision is improved; the biochemical detection image acquisition system can acquire quantitative detection images with high flux and high precision, has simple structure, convenient carrying and low cost, can meet the requirements of laboratories, hospitals and the like on biochemical detection, and can be widely popularized and applied in the fields of medical diagnosis, food safety, environmental supervision and the like.

Specifically, in the embodiment of the present invention, the portable electronic device may be a mobile phone, a tablet computer, a digital camera, or the like.

More specifically, as shown in fig. 2, in this embodiment, the portable electronic device is a smartphone 200, and the smartphone 200 has excellent image capturing capability and data processing capability. The smart phone 200 is provided with recording software, after the smart phone 200 captures an image of the optical fiber output array, the recording software performs image processing and data analysis to realize calibration test of a sample, processes the image and data in real time on a biochemical detection site, can be used smoothly in an environment where a network is not available, and can be applied in a wider scene.

Specifically, as shown in fig. 5, in this embodiment, the microlens array 3 further includes a plate body 32 having a rectangular shape as a whole, and a plurality of circular microlens 31 arrays are disposed on the plate body 32. The diameter of the microlens 31 is smaller than the diameter of the well 51 of the multi-well plate 5.

Specifically, in the embodiment of the present invention, the optical fiber 4 is a multimode optical fiber having a diameter of 600nm and has a transmission characteristic of transmitting visible light at a wavelength of 400nm to 700 nm.

Further, as shown in fig. 2, in the embodiment of the present invention, the biochemical detection image capturing system further includes a porous plate 5 detachably disposed in the first chamber 11, the porous plate 5 is located between the light source 2 and the microlens array 3, and centers of the plurality of well wells 51 are aligned with centers of the plurality of microlenses 31 one by one.

Specifically, as shown in fig. 6, in this embodiment, the multi-well plate 5 is a 96-well plate having 96 wells 51 in total of 8 × 12. Then, 96 optical fibers 4 are matched, and the end faces of the optical fibers 4 of the optical fiber output array are arranged in an 8 × 12 array; the microlens array 3 has 96 microlenses 31 of 8 × 12.

In addition, in embodiments not shown, the perforated plate 5 may also have more or fewer wells 51, for example the perforated plate 5 is a 24-well plate, a 48-well plate, a 384-well plate, or the like.

Specifically, as shown in FIG. 6, in this embodiment, the cross-sectional area of the fiber output array is 1/100 the cross-sectional area of the perforated plate 5. The cross section of the optical fiber output array is a section perpendicular to the length direction of the optical fiber 4, and the cross section of the porous plate 5 is a section perpendicular to the axial direction of the well 51. The reduction proportion of the cross-sectional area of the shot view field (namely, the optical fiber output array) compared with the perforated plate 5 is larger, when the portable electronic equipment is used for shooting, the edge distortion risk can be reduced, the image acquisition precision is improved, the imaging object distance can be reduced, the volume of a biochemical detection image acquisition system is further reduced, and the portability is improved.

Further, as shown in fig. 7, in the embodiment of the present invention, the light source 2 includes a plurality of light emitting elements 21 arranged in an array, the number of the light emitting elements 21 is the same as the number of the microlenses 31, the arrangement of the array of the light emitting elements 21 is the same as the arrangement of the microlens array 3, and the centers of the plurality of light emitting elements 21 are aligned with the centers of the plurality of microlenses 31 one by one. Through setting up the light emitting component 21 the same with microlens 31 quantity, the quantity of light emitting component 21 is the same with the quantity of the well 51 of perforated plate 5, and light emitting component 21 array aligns with microlens array 3, the light emitting component 21 array aligns with the well 51 array of perforated plate 5 promptly, every light emitting component 21 is located well 51 center directly over, every light emitting component 21 throws light on a well 51 alone, single well 51 can receive all light that single light emitting component 21 sent, reduce the loss of light intensity, improve the light utilization ratio, and then optical fiber 4 received light intensity is higher, further improve the light intensity of optical fiber 4 exit end, promote the image acquisition precision, and then improve detection sensitivity, be favorable to carrying out the quantitative determination of high accuracy.

Specifically, as shown in fig. 7, in the embodiment of the present invention, the Light Emitting element 21 is an LED (Light Emitting Diode) lamp bead, the Light source 2 further includes a substrate 22, and a plurality of LED lamp bead arrays are disposed on the substrate 22 to form an LED area array. Wherein, white light lamp pearl, blue light lamp pearl, ruddiness lamp pearl, green glow lamp pearl etc. can be selected to the LED lamp pearl.

More specifically, as shown in fig. 7, in this embodiment, the light source 2 further includes a protection resistor 23, the protection resistor 23 is disposed on the substrate 22 and electrically connected to the LED lamp bead, and the protection resistor 23 is used to protect the voltage of the LED lamp bead from being stable.

More specifically, as shown in fig. 7, in this embodiment, the light source 2 further includes a power supply interface 24, the power supply interface 24 is used to be electrically connected to an external power supply system, and the light source 2 may be powered by an external power supply, and does not need to use a battery, so as to reduce the occupied device space, facilitate reducing the volume of the biochemical detection image acquisition system, reduce the weight, and improve the portability. The power supply interface 24 may be a USB (Universal Serial Bus) interface, such as a Type-C (USB Type-C, an interface Type of a physical layer).

Specifically, as shown in fig. 2, in the embodiment of the present invention, at least two opposite inner sidewalls perpendicular to the top surface in the first chamber 11 are provided with a first supporting portion 111, and the light source 2 is supported on the first supporting portion 111 and detachably connected to the first supporting portion 111. Through set up the first supporting part 111 that is used for fixed light source 2 in first cavity 11, can with light source 2 demountable installation in first cavity 11, the replacement light source 2 is maintained to the convenience, and increase of service life can correspond according to the perforated plate 5 that uses moreover and change light source 2, can also select and change light source 2 according to the sample type that detects, light source 2's selectivity is strong, and reinforcing service function need not to customize camera bellows 1 again, and application scope is wider, and therefore, the carrier wave prepaid electric energy meter is high in cost and practicability.

More specifically, in this embodiment, the first supporting portion 111 is a rib or a boss protrudingly provided on opposite inner side walls of the first chamber 11; the two sides of the substrate 22 of the light source 2 are supported on the ribs or bosses on the two sides of the first chamber 11, and are fixedly connected with the ribs or bosses through screws.

Specifically, as shown in fig. 2, in this embodiment, an interface opening 19 is further formed on a side wall of the dark box 1 adjacent to the first supporting portion 111, and when the light source 2 is supported on the first supporting portion 111, the power supply interface 24 is embedded in the interface opening 19 and exposed from an outer side wall of the dark box 1, so as to electrically connect the power supply interface 24 with an external power supply.

Further, as shown in fig. 2, fig. 3 and fig. 4, in the embodiment of the present invention, the biochemical detection image collecting system further includes a double cemented lens 6, where the double cemented lens 6 is disposed in the optical path between the optical fiber output array and the window; the center of the doublet lens 6 is aligned with the center of the fiber output array. The double-cemented lens 6 can effectively eliminate distortion, shortens the imaging distance on the basis of ensuring excellent imaging, overcomes the problems of imaging edge distortion and large object distance requirement, and further can reduce the height required by the second chamber 12, thereby reducing the whole volume of the biochemical detection image acquisition system and improving the portability.

Furthermore, as shown in fig. 2, in this embodiment, the image collecting system for biochemical detection further includes a lens barrel 60, the lens barrel 60 is disposed in the second chamber 12 and is fixedly connected to the inner wall of the top surface of the camera bellows 1 around the window, and the double cemented lens 6 is disposed in the lens barrel 60. Through lens cone 60 with double cemented lens 6 fixed mounting in second cavity 12 and with the window alignment, lens cone 60's shading nature and closure are good simultaneously, can prevent light leakage and light loss, guarantee the image acquisition precision, satisfy the demand to accurate quantitative measurement.

Specifically, in this embodiment, the doublet lens 6 has a diameter of 25.4mm and a focal length of 25 mm; the doublet lens 6 is fixed in the barrel 60 using a snap ring.

Specifically, in the embodiment of the present invention, the image information captured by the portable electronic device through the biochemical detection image capturing system of the embodiment of the present invention can be combined with colorimetric detection, and is widely applied to the field of portable biochemical detection. The colorimetric detection is a common biochemical sensing detection means, and is calibrated by analyzing the colorimetric and chromogenic effects of a detected object.

Further, as shown in fig. 2, fig. 3 and fig. 4, in the embodiment of the present invention, the biochemical detection image capturing system further includes a first optical insertion sheet 71, and the first optical insertion sheet 71 is detachably disposed in the optical path between the light source 2 and the multi-well plate 5. The first optical insertion sheet 71 may be a light homogenizing sheet, such as frosted glass, which can convert the light emitted by the light source 2 into uniform light, and the wavelength of the uniform light does not change, and the uniform light directly irradiates the sample in the porous plate 5 for colorimetric detection; the first optical insert 71 may also be a filter capable of converting light emitted from the light source 2 into light of a specific wavelength for colorimetric detection. Through setting up first optics inserted sheet 71, and first optics inserted sheet 71 can be dismantled, through the first optics inserted sheet 71 of changing the different grade type, can realize that multiple different colorimetry detects, satisfies the biochemical detection needs to multiple sample.

Specifically, as shown in fig. 2, in the embodiment of the present invention, at least two opposite inner sidewalls perpendicular to the top surface in the first chamber 11 are further provided with a second supporting portion 112, and the first optical insert 71 is supported on the second supporting portion 112 and detachably connected to the second supporting portion 112; the second supporting portion 112 is located at the lower side of the first supporting portion 111 to dispose the first optical insert 71 below the light source 2; when the porous plate 5 is received in the first chamber 11, the second support portion 112 is located at an upper side of the porous plate 5 to dispose the first optical insert 71 above the porous plate 5. Through set up second supporting part 112 in first cavity 11, realize being fixed in first cavity 11 with first optics inserted sheet 71 demountable installation in, first optics inserted sheet 71 is changed swiftly conveniently.

More specifically, in this embodiment, the second supporting portion 112 is a rib or a boss protruding from two opposite inner sidewalls of the first chamber 11; both sides of the ground glass or the optical filter are supported on the ribs or bosses on both sides of the first chamber 11 and fixed on the ribs or bosses using screws.

Furthermore, as shown in fig. 2, fig. 3 and fig. 4, in the embodiment of the present invention, the biochemical detection image capturing system further includes a second optical insertion sheet 72, the second optical insertion sheet 72 is detachably disposed in the optical path between the multi-well plate 5 and the microlens array 3, and the second optical insertion sheet 72 is a filter. Fluorescence detection is also a common biochemical sensing detection means, and is calibrated by analyzing the colorimetric, chromogenic and fluorescent effects of a detected object. The difference between the optical paths for fluorescence detection and colorimetric detection is that in the fluorescence method, light passes through a sample to be detected and then needs to pass through an emission filter to obtain fluorescence of a certain wavelength. By arranging the optical filter for fluorescence emission in the light path between the porous plate 5 and the micro-lens array 3, the image information captured by the biochemical detection image acquisition system of the portable electronic equipment can be combined with fluorescence detection, so that the fluorescence detection is realized, and the portable electronic equipment is widely applied to the field of portable biochemical detection; the second optical insertion sheet 72 can be detached, the second optical insertion sheet 72 and the first optical insertion sheet 71 are combined for use, the two detection modes of color comparison and fluorescence can be switched by flexibly assembling optical filters for excitation and emission, the biochemical sensing detection of the two modes of color comparison and fluorescence is realized, and the use function is enhanced.

Specifically, in the embodiment of the present invention, when the selection mode is the colorimetric detection method, the first optical insertion sheet 71 is inserted into the optical path between the light source 2 and the multi-well plate 5, and the first optical insertion sheet 71 is a light homogenizing sheet or a filter; when the selection mode is a fluorescence detection method, a first optical insert 71 is inserted into the light path between the light source 2 and the porous plate 5, a second optical insert 72 is inserted into the light path between the porous plate 5 and the microlens array 3, the first optical insert 71 is a first optical filter and is used as an excitation optical filter, and the second optical insert 72 is a second optical filter and is used as an emission optical filter.

More specifically, in this embodiment, the light uniformizing sheet is ground glass.

Further, as shown in fig. 2, in the embodiment of the present invention, a third chamber 13 is further disposed inside the dark box 1, the third chamber 13 is located below the first chamber 11 and the second chamber 12, the third chamber 13 is communicated with the first chamber 11 and the second chamber 12, and the plurality of optical fibers 4 are all accommodated in the third chamber 13. Through setting up third chamber 13, light-proofness and closure are good, prevent light leak and light intensity loss, satisfy the demand to accurate quantitative measurement.

Further, as shown in fig. 8, in the embodiment of the present invention, the biochemical detection image collecting system further includes a fiber array panel 8, the fiber array panel 8 includes an incident array 81 and an exit array 82, the incident array 81 has a plurality of incident holes 83 arranged in an array, the number of the incident holes 83 is the same as that of the optical fibers 4, and the arrangement of the incident hole 83 array is the same as that of the microlens array 3; exit array 82 has a plurality of exit holes 84 arranged in an array, and the number of exit holes 84 is the same as the number of optical fibers 4. As shown in fig. 2, the fiber array panel 8 is disposed on the top of the third chamber 13 and encloses the bottoms of the first chamber 11 and the second chamber 12, such that the incident array 81 is located at the bottom of the first chamber 11, the emergent array 82 is located at the bottom of the second chamber 12, the centers of the plurality of incident holes 83 are aligned with the centers of the plurality of microlenses 31 one by one, and the center of the emergent array 82 is aligned with the center of the window; one end of each optical fiber 4 of the plurality of optical fibers 4 is fixedly connected to the penetration hole 83, and the other end is fixedly connected to the emergence hole 84, so that two end faces of the plurality of optical fibers 4 are respectively arranged to form an optical fiber input array and an optical fiber output array. The installation of a plurality of optical fibers 4 is fixed by arranging the optical fiber array panel 8, the structure is simple, the disassembly and the assembly are fast and convenient, the position stability of the optical fiber input array and the optical fiber output array is ensured, and the image acquisition precision is ensured.

Specifically, as shown in fig. 8, in this embodiment, an annular support portion 85 is disposed on the fiber array panel 8 around the incident array 81, and the annular support portion 85 is used to support the microlens array 3, so that the microlens array 3 is spaced apart from the incident array 81, and also to facilitate the microlenses 31 to focus light on the end faces of the optical fibers 4. For example, the distance between the center of the microlens 31 and the center of the entrance hole 83 is equal to the focal length of the microlens 31.

Specifically, as shown in fig. 8, in this embodiment, an annular limiting portion 86 is further disposed on the fiber array panel 8 around the annular supporting portion 85, the annular limiting portion 86 surrounds a limiting groove, the microlens array 3 is embedded in the limiting groove, the annular limiting portion 86 limits the relative position of the microlens array 3 and the incidence array 81, and ensures that the centers of the microlenses 31 and the centers of the incidence holes 83 are aligned one by one, thereby ensuring the measurement accuracy.

More specifically, as shown in fig. 8, in this embodiment, a groove 87 is concavely formed on the plate surface of the fiber array panel 8, and the array of incident holes 83 of the incident array 81 is disposed in the groove 87; the plate surface of the fiber array panel 8 around the outer periphery of the groove 87 forms the annular support portion 85.

More specifically, as shown in fig. 2, in this embodiment, the second optical insert 72 is embedded in a position-limiting groove surrounded by a ring-shaped position-limiting portion 86 on the fiber array panel 8 and is located above the microlens array 3.

Specifically, as shown in fig. 8, in this embodiment, an annular clamping groove 88 is further disposed on the fiber array panel 8 around the periphery of the annular limiting portion 86, a clamping portion is disposed at the bottom of the porous plate 5, and the annular clamping groove 88 is used for being matched and clamped with the clamping portion at the bottom of the porous plate 5 to fixedly push the porous plate 5 in the first chamber 11. For example, the ring slot 88 is rectangular.

More specifically, in this embodiment, the fiber array panel 8 is a stainless steel plate, and the fiber array panel 8 is fixed to the top of the third chamber 13 by screws.

Specifically, as shown in fig. 2, in the embodiment of the present invention, the camera bellows 1 includes a bellows body 14 and a magnetic side door 15, the first chamber 11, the second chamber 12, and the third chamber 13 are formed in the bellows body 14, a side surface of the bellows body 14 perpendicular to a top surface thereof is opposite to the first chamber 11 and is provided with a strip-shaped opening 16, the strip-shaped opening 16 forms a strip-shaped channel communicating with the first chamber 11, and the strip-shaped opening 16 is used for detachably mounting the porous plate 5 in the first chamber 11; the magnetic side door 15 is magnetically connected with the box body 14 and closes the strip-shaped opening 16. As shown in fig. 9, when the magnetically-attracted side door 15 is removed, the first chamber 11 is in an open state, and the porous plate 5 is inserted into the first chamber 11 through the strip-shaped opening 16 or taken out from the first chamber 11; when closing magnetic absorption side door 15, first cavity 11 is in the encapsulated situation, can carry out formal test process this moment, and light-proofness and closure are good, prevent light leak and light loss, satisfy the demand to accurate quantitative measurement.

Specifically, in this embodiment, the back surface of the magnetic side door 15 is provided with a strong magnetic patch, and the magnetic side door 15 can be adsorbed on the side wall of the box 14 around the strip-shaped opening 16 through the strong magnetic patch, so as to open and close the first chamber 11.

More specifically, as shown in fig. 9, in this embodiment, the second supporting portion 112 is provided with a magnetic attraction member 113 towards the outer edge of the strip-shaped opening 16, the magnetic attraction member 113 may be a strong magnetic patch, and the strong magnetic patch on the back of the magnetic side door 15 is magnetically engaged with the magnetic attraction member 113, so as to magnetically attach the magnetic side door 15 to the box 14.

Specifically, as shown in fig. 8 and 9, in this embodiment, the side of the fiber array panel 8 adjacent to the strip-shaped opening 16 is provided with a relief groove 89, for example, the relief groove 89 is a trapezoidal groove; the groove 89 of stepping down is used for dodging to get the instrument of getting, gets the instrument and can place and take perforated plate 5 in groove 89 department of stepping down.

Further, as shown in fig. 2, in the embodiment of the present invention, the black box 1 further includes a magnetic baffle 17, at least one side surface of the box body 14 perpendicular to the top surface thereof faces the third chamber 13 and is provided with a third chamber opening 18 communicated with the third chamber 13; the magnetically attractive baffle 17 is magnetically attracted to the housing 14 and closes the third chamber opening 18. By providing the third chamber opening 18, when the magnetically attracting baffle 17 is removed, the third chamber 13 is opened, and the position of the optical fiber 4 can be checked and adjusted; when installing magnetism and inhale baffle 17, third chamber 13 is in the encapsulated situation, can carry out formal test process this moment, and light-proofness and closure are good, prevent light leak and light loss, satisfy the demand to accurate quantitative measurement.

Specifically, as shown in fig. 2, in this embodiment, the camera bellows 1 includes two magnetically attracting baffles 17, and third chamber openings 18 are opened at two opposite sides of the bellows body 14.

More specifically, in this embodiment, the back of the magnetic attraction baffle 17 is provided with a strong magnetic patch, the magnetic attraction baffle 17 can be temporarily adsorbed on the side wall of the box 14 in the pre-testing and adjusting stage, and the magnetic attraction baffle 17 is fastened and connected with the box 14 through a screw after the position of the optical fiber 4 bundle is confirmed to be error-free.

In one embodiment, as shown in fig. 2, the box 14 includes an upper box 141, a lower box 142 and a top cover 143, two cavities penetrating in the height direction are formed in the upper box 141, and the two cavities are separated by a partition to avoid light influence; a strip-shaped through hole is formed in the side wall of one cavity, which is far away from the other cavity, and the through hole is a strip-shaped opening 16; a cavity with an open top is formed in the lower box body 142, and through holes, namely the third chamber opening 18, are formed on two opposite side walls of the lower box body 142; the top cover 143 is also provided with a through hole, which is a window; the upper box body 141 is fixedly connected to the top of the lower box body 142 through screws, and the top cover 143 is fixedly connected to the top of the upper box body 141 through screws and closes the top opening of the cavity of the upper box body 141; thus, the top cover 143 and the cavity of the upper case 141 opened with the strip-shaped opening 16 define a first chamber 11, the top cover 143 and the other cavity of the upper case 141 define a second chamber 12, and the cavity in the lower case 142 defines a third chamber 13.

Further, as shown in fig. 1 and fig. 2, in the embodiment of the present invention, the biochemical detection image capturing system further includes a mounting member 9, the mounting member 9 is detachably connected to the top of the dark box 1, and the mounting member 9 is used for fixedly mounting the portable electronic device and making the camera 201 of the portable electronic device face the window. Through the arrangement of the detachable installation part 9, the installation part 9 can be provided with various specifications, the biochemical detection image acquisition system can be used in combination with the existing portable electronic equipment with various models, and can also be used in combination with other new portable electronic equipment, if the portable electronic equipment for detection is replaced, the whole camera bellows 1 does not need to be customized or changed again, only the new installation part 9 needs to be customized, the cost is reduced, the applicability is stronger, and the application scene is wide.

Specifically, as shown in fig. 2, in this embodiment, the mounting member 9 is provided with a mounting groove 91, the shape and size of the mounting groove 91 are adapted to those of the portable electronic device, and the mounting groove 91 is used for mounting the portable electronic device; the bottom of the mounting groove 91 is opposite to the window and is provided with a camera opening 92, and the camera opening 92 is used for exposing the camera 201 of the portable electronic device. The mounting part 9 is customized according to the used portable electronic equipment, the adaptability is better, the mounting groove 91 is strictly the same as the size of the portable electronic equipment, the mounting of the portable electronic equipment is firm and stable, and the image acquisition precision is ensured.

Specifically, as shown in fig. 2, in this embodiment, the side edge of the mounting groove 91 is further provided with an arc-shaped groove 93, and the arc-shaped groove 93 is used for placing and taking the portable electronic device, so that the operation is convenient.

More specifically, in this embodiment, the mounting member 9 is a mounting plate, the mounting member 9 is fixedly attached to the top cover 143 on the top of the camera bellows 1 by screws, and the imaging opening 92 of the mounting member 9 is concentrically aligned with the window on the top surface of the camera bellows 1.

The biochemical detection image acquisition system of the present invention will be specifically described below by taking as an example a biochemical detection image acquisition system that is used in combination with the smartphone 200, is suitable for a 96-well plate, and has a colorimetric and fluorescent dual mode.

In the present embodiment, the overall size of the dark box 1 is 200mm × 100mm × 150mm, and the dark box is small in size and excellent in portability. A96-well plate has 96 wells 51 in total of 8X 12. The number of the optical fibers 4 is 96.

Light source 2 is the LED area array, is equipped with 96 LED lamp pearls totally 8X 12, a 1 omega's protective resistor 23 and a Type-C power supply interface 24, and the parameter of every LED lamp pearl is: rated voltage is 3V, rated current is 0.02A, and total lighting power is 5.76W; the light source 2 is connected into the power supply system through a Type-C power supply line with a switch.

The microlens array 3 has 96 microlenses 31 of 8 × 12, the diameter of the microlens 31 is not more than 7mm, the focal length is 0.5mm, the thickness of the lens is 0.6mm, and the pitch of the microlens 31 is 9 mm.

The diameter of the doublet lens 6 is 25.4mm, and the focal length is 25 mm.

The size of the incident array 81 of the optical fiber array panel 8 is 110mm × 72mm, 96 incident holes 83 with the diameter of 8 × 12 are arranged, the incident holes 83 are through holes with the diameter of 0.6mm, and the distance between the incident holes 83 is 9 mm; the size of exit array 82 is 12.8mm × 8.55mm, 96 exit holes 84 of 8 × 12 are provided, exit holes 84 are through holes with a diameter of 0.6mm, and the distance between exit holes 84 is 0.9 mm. The area of exit array 82 is 1/100 of the cross-sectional area of a 96-well plate.

The pixels of the rear camera 201 of the smart phone 200 exceed 1000 ten thousand, and the screen resolution is not lower than 1080P (1920 × 1080). The smartphone 200 is equipped with colorimetric and fluorescent dual-mode sensing detection recording software.

As shown in fig. 3, inside the dark box 1, 96 lamp beads of the LED area array, 96 well wells 51 of the 96-well plate, 96 microlenses 31 of the microlens array 3, and 96 incident holes 83 of the incident array 81 of the fiber array panel 8 are arranged in vertical central alignment; the exit array 82 of the fiber array panel 8 is vertically aligned with the double cemented lens 6 and the camera 201 of the smartphone 200.

When a colorimetric method is adopted, frosted glass or an optical filter is arranged between the LED area array and the 96-pore plate, when frosted glass is arranged, light emitted by the LED area array is converted into uniform light, the wavelength is not changed, and a sample is directly irradiated for detection; when the optical filter is arranged, the light emitted by the LED area array is converted into light with specific wavelength for colorimetric detection. The dimensions of the ground glass and the filter are 130mm x 88mm, and the thickness does not exceed 1.5 mm.

When a fluorescence method is adopted, a first optical filter is arranged between the LED area array and the 96-hole plate and used as an excitation optical filter, the size of the first optical filter is 130mm multiplied by 88mm, and the thickness of the first optical filter is not more than 1.5 mm; a second filter is arranged between the 96-well plate and the micro-lens array 3 and used as an emission filter, and the size of the second filter is 110mm multiplied by 72mm, and the thickness of the second filter is not more than 1.5 mm.

As shown in fig. 10, based on the biochemical detection image acquisition system provided in the above embodiment, an embodiment of the present invention further provides a biochemical detection image acquisition method, which specifically includes the following steps:

s101, determining parameters of the light source 2 according to the characteristics of the object to be detected and the requirements of biochemical detection experiments. The light source 2 is selected according to the determined parameters of the light source 2 and the light source 2 is fixedly mounted in the first chamber 11 of the camera bellows 1 by means of the first support 111.

S102, determining the test mode to be a colorimetric method or a fluorescence method according to the characteristics of the object to be tested and the requirements of biochemical test experiments, and determining the parameters of the first optical insert 71 and the second optical insert 72 according to the test mode.

If the fluorescence method is selected, the excitation filter is fixed in the first chamber 11 of the dark box 1 through the second supporting part 112, the emission filter is embedded in the fiber array panel 8, and parameters of the excitation filter and the emission filter are determined. If a colorimetric method is selected, whether light with a specific wavelength is needed to be illuminated is judged, and if the light with the specific wavelength is not needed to be illuminated, frosted glass is selected to be fixed at the second supporting part 112 in the first chamber 11 of the dark box 1; if illumination with a specific wavelength is required, the filter is fixed in the first chamber 11 of the dark box 1 through the second support part 112, and the parameters of the filter are determined.

S103, a sample of the object to be measured is prepared and stored in the plurality of wells 51 of the multi-well plate 5.

S104, confirming that the light source 2 is in a closed state, feeding the porous plate 5 into the first chamber 11, and confirming that the dark box 1 is in a closed state.

Before the start of the official experiment, it is necessary to determine that the light source 2 is in the off state. Opening the magnetic side door 15, clamping the side edge of the porous plate 5 by using tweezers, stably feeding the porous plate 5 into the first chamber 11, and taking out the tweezers after the bottom of the porous plate 5 stably falls into the annular clamping groove 88 on the optical fiber array panel 8; after the multi-well plate 5 is placed, the biochemical detection image acquisition system is shown in fig. 9. Then the magnetic side door 15 and the magnetic baffle 17 are magnetically connected to the box body 14 to seal the strip-shaped opening 16 and the third chamber opening 18, so as to ensure that the interior of the camera bellows 1 is totally closed.

S105, the portable electronic equipment with the camera 201 is arranged on the top of the dark box 1, the camera 201 is enabled to face the window, and the camera 201 is opened.

And opening a photographing mode of corresponding recording software in the smart phone 200, and entering a standby working mode.

S106, turning on the light source 2, performing focusing operation on the camera 201 on the portable electronic equipment, and performing photographing operation after the camera 201 is successfully focused to obtain a biochemical detection image.

Turning on a switch on a power supply line to turn on all LED lamp beads of a light source 2, wherein at the moment, as shown in figure 4, light emitted by an LED lamp bead area array is converted into uniform light or light with special wavelength after passing through ground glass or an optical filter, after the light beam penetrates through a porous plate 5 filled with a sample (colorimetric solution) of a measured object, a light beam is coupled into one end of a multimode optical fiber 4 by a micro-lens array 3, and the optical fiber 4 transmits an optical signal to the other end to form a light-emitting emergent array 82; focusing operation is carried out on the smart phone 200, a shutter is pressed when focusing is successful, photographing is completed, the emergent light beam is focused on the CMOS sensor by the double-cemented lens 6 and the camera 201 of the smart phone 200 to form image information, and therefore a biochemical detection image is obtained.

S107, the LED lamp beads of the light source 2 are turned off, the porous plate 5 is clamped out by using tweezers, and the first chamber 11 of the camera bellows 1 is restored to a closed state.

Finally, on the smart phone 200, image and data processing is performed through the carried recording software, and the content calibration of the measured object is obtained by combining the calibration curve, so that the calibration test of the sample is realized.

The biochemical detection image acquisition system provided by the embodiment of the invention is simple and convenient to use, and has the advantages of large flux, double modes, high precision, portability and wide application scene.

In a specific embodiment, when the colorimetric method is selected for detecting pyrophosphate, the light emitting element 21 in the light source 2 is a white light LED with a spectral range of 400nm to 700nm, the first optical insert 71 is ground glass, and the camera 201 of the smartphone 200 performs RGB three-channel color analysis after imaging, and performs content calibration by combining a calibration curve.

When a colorimetric method is selected for detecting pathogenic bacteria, the light-emitting element 21 in the light source 2 is a blue light LED with a spectral range of 464nm, the first optical insertion sheet 71 is made of ground glass, the camera 201 of the smart phone 200 captures RGB three-channel color information after imaging and converts the RGB three-channel color information into a gray value, and content calibration is performed by combining a calibration curve.

When a colorimetric method is selected for detecting protein, the light-emitting element 21 in the light source 2 is a white light LED with a spectral range of 400nm-700nm, the first optical insertion sheet 71 is a narrow-band filter with parameters of 615nm, the camera 201 of the smart phone 200 captures RGB three-channel color information after imaging, and content calibration is performed by combining a calibration curve.

When the fluorescence method is selected for detecting nucleic acid, the light-emitting element 21 in the light source 2 is a white light LED with a spectral range of 400nm-700nm, the first optical insertion sheet 71 is an excitation optical filter with parameters of 465nm, the second optical insertion sheet 72 is an emission optical filter with parameters of 530nm, the camera 201 of the smart phone 200 captures RGB three-channel color information after imaging, and content calibration is performed only by using green channel information and combining a calibration curve.

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. A biochemical detection image acquisition system, comprising:

a light source disposed at a top portion within the first chamber;

2. The biochemical detection image capturing system according to claim 1,

the cross-sectional area of the fiber output array is 1/100 the cross-sectional area of the perforated plate.

3. The biochemical detection image capturing system according to claim 1,

biochemical detection image acquisition system still includes first optics inserted sheet, first optics inserted sheet can dismantle the setting and be in the light source with in the light path between the perforated plate.

4. The biochemical detection image capturing system according to claim 3,

biochemical detection image acquisition system still includes second optics inserted sheet, second optics inserted sheet can dismantle the setting and be in the perforated plate with in the light path between the microlens array.

5. The biochemical detection image capturing system according to claim 1,

the biochemical detection image acquisition system also comprises an optical fiber array panel, wherein the optical fiber array panel comprises an incident array and an emergent array, the incident array is provided with a plurality of incident holes arranged in an array, the arrangement of the incident holes is the same as that of the hole wells on the perforated plate, and the emergent array is provided with a plurality of emergent holes arranged in an array; the optical fiber array panel is arranged at the bottoms of the first cavity and the second cavity, the incident array is positioned in the first cavity, the emergent array is positioned in the second cavity, and the centers of the incident holes are aligned with the centers of the micro lenses one by one; one end of each optical fiber in the optical fibers is fixedly connected to the incident hole, and the other end of each optical fiber is fixedly connected to the emergent hole;

6. Biochemical detection image acquisition system according to claim 5,

the optical fiber array panel is surrounded the annular supporting part is also provided with an annular clamping groove, and the annular clamping groove is used for being matched and clamped with the bottom of the porous plate.

7. The biochemical detection image capturing system according to any one of claims 1 to 6,

at least two opposite inner side walls perpendicular to the top surface in the first chamber are provided with first supporting parts, and the light source is supported on the first supporting parts and is detachably connected with the first supporting parts.

8. The biochemical detection image capturing system according to claim 7,

the light source further comprises a power supply interface, an interface opening is further formed in the side wall, adjacent to the first supporting portion, of the camera bellows, and the power supply interface is embedded in the interface opening and is exposed out of the outer side wall of the camera bellows.

9. The biochemical detection image capturing system according to claim 3,

still be provided with the second supporting part on the relative two inside walls of perpendicular to top surface at least in the first cavity, first optics inserted sheet support in on the second supporting part, and with the connection can be dismantled to the second supporting part.

10. A biochemical detection image acquisition method is characterized by comprising the following steps: