CN114088706B

CN114088706B - Biochemical detection image acquisition system and image acquisition method

Info

Publication number: CN114088706B
Application number: CN202111266001.7A
Authority: CN
Inventors: 耿照新; 苗焱蕊
Original assignee: Minzu University of China
Current assignee: Minzu University of China
Priority date: 2021-10-28
Filing date: 2021-10-28
Publication date: 2024-04-26
Anticipated expiration: 2041-10-28
Also published as: CN114088706A

Abstract

The invention provides a biochemical detection image acquisition system and an image acquisition method, wherein the biochemical detection image acquisition system comprises a camera bellows, a light source, a micro lens array and a plurality of optical fibers, wherein a first cavity and a second cavity are arranged in the camera bellows at intervals along the direction vertical to the height, the first cavity is used for accommodating a porous plate with a plurality of holes arranged in an array, a window is arranged at the position, opposite to the second cavity, of the top surface of the camera bellows, the window penetrates into the second cavity, and the top of the camera bellows is used for installing portable electronic equipment with a camera; the light source is arranged at the top part in the first cavity; one end of each optical fiber in the plurality of optical fibers is fixed at the bottom in the first cavity to form an optical fiber input array, and the other end of each optical fiber is fixed at the bottom in the second cavity to form an optical fiber output array; the microlens array is disposed above the optical fiber input array. The biochemical detection image acquisition system can acquire quantitative detection images with large flux and high precision, and is simple in structure and convenient to carry.

Description

Biochemical detection image acquisition system and image acquisition method

Technical Field

The invention relates to the technical field of biochemical detection, in particular to a biochemical detection image acquisition system and an image acquisition method.

Background

The detection of food allergens, urine analysis, blood analysis, water quality monitoring and other fields are widely performed by using a colorimetric method and a fluorescence method. The traditional colorimetric method is used for analyzing the content of the measured object through naked eyes or a spectrophotometer, and is simple and convenient to operate and visual in experimental effect. The fluorescence detection method is used as a special colorimetric method, and the main principle is that molecules of certain substances are in an excited state after being irradiated by light with specific wavelength, and the molecules can be qualitatively or quantitatively analyzed through specific fluorescence emitted by collision and de-excitation processes. Traditional spectrometers for colorimetry and fluorescence are difficult to use widely in poor or remote areas due to the large size and high cost of the spectrometers.

Current smartphones are typically equipped with a fast multi-core processor, a touch screen, a high capacity battery, and a variety of elements (e.g., optical imaging system cameras, accelerometers, hygrometers, fingerprint scanners, heart rate sensors, etc.) that can be used for detection and measurement, and smartphones have been upgraded from original portable communication tools to microcomputers, with the characteristics of portability, low cost, easy operation, and high computational power. The smart phone is combined with various laboratory level detection means, so that a powerful tool can be provided for scientific experiments and clinical instant diagnosis. For example, the ability of a smartphone to capture color may be combined with colorimetric and fluorescent methods. A smart phone equipped with CMOS (Complementary Metal Oxide Semiconductor ) sensor, capable of capturing optical signals and converting into RGB (red, green, blue) color system value ranges (0-255) by built-in algorithms; through a calibration curve and a specific data processing method, the RGB intensity value corresponds to the content of the measured object, so that qualitative or quantitative calibration is realized. The smart phone is combined with the carefully designed hardware equipment, so that the detection result level which is not inferior to that of a spectrometer can be achieved, the smart phone is convenient to carry, and the high-precision detection of the sample on site is realized. At present, smartphones have been widely used in food, environmental, agricultural and medical testing.

The biochemical detection system adopts a 96-well plate as an experimental instrument, has the characteristics of large flux and extremely high detection efficiency, and can perform detection experiments of multiple flux and small samples. When the existing colorimetric or fluorescent method is combined with a smart phone, the smart phone is generally used for directly shooting a 96-well plate, and then the obtained photo is subjected to image processing and qualitative and quantitative calculation. However, the cross-sectional area of the 96-well plate is relatively large, and when the intelligent mobile phone is used for shooting, a large object distance is needed to ensure that the 96-well plate is completely placed in the view-finding frame, so that the longitudinal size is required to be large, and the portability of the equipment is influenced; when the intelligent mobile phone shoots the 96-well plate by using the wide-angle lens, the edges of the shot image are easy to distort in a limited size due to the large imaging area, so that the detection sensitivity is low, and accurate quantitative detection is difficult to perform; in addition, the smooth surface of the 96-well plate can reflect light, and the requirements on a sample darkroom and an image preprocessing process are high. Therefore, there is a need for a portable high-precision biochemical detection image acquisition system and method that can reduce the image preprocessing requirements.

Disclosure of Invention

The invention aims to provide a biochemical detection image acquisition system and an image acquisition method, which are used for solving the technical problems of large volume, poor portability, low detection sensitivity and high requirements on an image preprocessing process of the conventional colorimetric array image acquisition equipment.

In order to solve the above technical problems, the present invention provides a biochemical detection image acquisition system, including:

the camera comprises a camera body, a camera, a box body, a first cavity and a second cavity, wherein the first cavity and the second cavity are arranged in the camera body at intervals along the direction vertical to the height, the first cavity is used for accommodating a porous plate with a plurality of holes arranged in an array, a window is arranged at the position, opposite to the second cavity, of the top surface of the camera body, the window penetrates into the second cavity, and the top of the camera body is used for installing portable electronic equipment with a camera;

a light source disposed at a top portion within the first chamber;

One end of each of the plurality of optical fibers is fixed at the bottom in the first chamber to form an optical fiber input array, the arrangement of the end faces of the optical fibers in the optical fiber input array is the same as the arrangement of the hole wells on the porous plate, and the other end of each of the plurality of optical fibers is fixed at the bottom in the second chamber to form an optical fiber output array;

The micro lens array is provided with a plurality of micro lenses arranged in an array mode, the micro lens array is arranged in the first cavity and is positioned above the optical fiber input array, and the centers of the micro lenses are aligned with the end face centers of the optical fibers of the optical fiber input array one by one.

According to the biochemical detection image acquisition system provided by the invention, the cross section area of the optical fiber output array is 1/100 of the cross section area of the porous plate.

According to the biochemical detection image acquisition system provided by the invention, the biochemical detection image acquisition system further comprises a first optical insertion sheet, and the first optical insertion sheet is detachably arranged in a light path between the light source and the porous plate.

According to the biochemical detection image acquisition system provided by the invention, the biochemical detection image acquisition system further comprises a second optical insert which is detachably arranged in a light path between the porous plate and the micro lens array.

According to the biochemical detection image acquisition system provided by the invention, the biochemical detection image acquisition system further comprises an optical fiber array panel, wherein the optical fiber array panel comprises an incident array and an emergent array, the incident array is provided with a plurality of incident holes arranged in an array, the arrangement of the incident holes is the same as that of the hole wells on the porous plate, and the emergent array is provided with a plurality of emergent holes arranged in an array; the optical fiber array panel is arranged at the bottoms of the first cavity and the second cavity, the incident array is positioned in the first cavity, the emergent array is positioned in the second cavity, and the centers of the plurality of incident holes are aligned with the centers of the plurality of microlenses one by one; one end of each optical fiber in the plurality of optical fibers is fixedly connected with the entrance hole, and the other end is fixedly connected with the exit hole;

An annular supporting part is arranged on the optical fiber array panel around the incidence array and is used for supporting the micro lens array.

According to the biochemical detection image acquisition system provided by the invention, the optical fiber array panel is further provided with the annular clamping groove around the annular supporting part, and the annular clamping groove is used for being matched and clamped with the bottom of the porous plate.

According to the biochemical detection image acquisition system provided by the invention, the first supporting parts are arranged on at least two opposite inner side walls perpendicular to the top surface in the first chamber, and the light source is supported on the first supporting parts and detachably connected with the first supporting parts.

According to the biochemical detection image acquisition system provided by the invention, the light source further comprises a power supply interface, an interface opening is further formed in the side wall, adjacent to the first supporting part, of the camera bellows, and the power supply interface is embedded and installed in the interface opening and is exposed from the outer side wall of the camera bellows.

According to the biochemical detection image acquisition system provided by the invention, the first chamber is internally provided with the second supporting parts on at least two opposite inner side walls perpendicular to the top surface, and the first optical inserting sheet is supported on the second supporting parts and detachably connected with the second supporting parts.

The invention also provides a biochemical detection image acquisition method, which comprises the following steps:

determining the light source parameters of a biochemical detection image acquisition system according to the characteristics of the detected object and biochemical detection experiment requirements;

according to the characteristics of the detected object and the biochemical detection experimental requirements, parameters of a first optical inserting sheet and a second optical inserting sheet of a biochemical detection image acquisition system are determined;

preparing a sample of the object to be measured, and storing the sample of the object to be measured in a plurality of wells of a porous plate;

Confirming that the light source is in a closed state, sending the porous plate into a first cavity of the biochemical detection image acquisition system, and confirming that a camera bellows of the biochemical detection image acquisition system is in a closed state;

mounting the portable electronic equipment with the camera on the top of the camera box, enabling the camera to face the window, and opening the camera;

And turning on the light source, performing focusing operation of the camera on the portable electronic equipment, and performing photographing operation after the camera is focused successfully to obtain a biochemical detection image.

According to the biochemical detection image acquisition system and the biochemical detection image acquisition method, the optical fiber array is arranged, so that the shot view field can be obviously reduced, shooting and imaging of portable electronic equipment are facilitated, the limitation of focusing distance is reduced, the volume of an optical structure is reduced, the volume of the biochemical detection image acquisition system is further reduced, and portability is improved; through set up the microlens array above optic fibre input array, the microlens can be with the whole collection of Kong Jingde portion outgoing light to the input terminal surface of optic fibre in, promote the receipts light efficiency of optic fibre greatly, strengthen the intensity of the light in the coupling optic fibre, improve the coupling efficiency of light, promote the light intensity of optic fibre output light beam, improve the image acquisition precision, thereby promote detection sensitivity and accuracy, and then improve quantitative measurement precision, can carry out the quantitative detection image acquisition of large flux, high accuracy, moreover simple structure, portable, with low costs, can satisfy laboratory, hospital etc. to the needs of biochemical detection, can extensively popularize and apply in fields such as medical diagnosis, food safety, environmental supervision.

Drawings

In order to more clearly illustrate the invention or the technical solutions of the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic perspective view of a biochemical detection image acquisition system according to an embodiment of the present invention;

FIG. 2 is a schematic diagram showing an exploded structure of a biochemical detection image capturing system according to an embodiment of the present invention;

FIG. 3 is a schematic diagram showing the mating positions of a light source, a first optical insert, a multi-well plate, a second optical insert, a micro lens array, a fiber array panel, a fiber, a double-cemented lens, and a portable electronic device according to an embodiment of the present invention;

FIG. 4 is a schematic view of an optical path of a biochemical detection image capturing system according to an embodiment of the present invention when in operation;

FIG. 5 is a schematic view showing the structure of a microlens array according to an embodiment of the present invention;

FIG. 6 is a schematic diagram of area comparison of a multi-well plate and an exit array according to an embodiment of the present invention;

FIG. 7 is a schematic view of a light source according to an embodiment of the present invention;

FIG. 8 is a schematic diagram of an optical fiber array panel and optical fiber assembly according to an embodiment of the present invention;

FIG. 9 is a schematic diagram showing an opened state of a magnetic side door of the biochemical detection image capturing system according to the embodiment of the present invention;

FIG. 10 is a flowchart of a biochemical test image capturing method according to an embodiment of the present invention.

In the figure:

1. A camera bellows; 11. a first chamber; 111. a first support portion; 112. a second supporting part; 113. a magnetic attraction piece; 12. a second chamber; 13. a third chamber; 14. a case; 141. an upper case; 142. a lower box body; 143. a top cover; 15. a magnetic side door; 16. a strip-shaped opening; 17. a magnetic baffle; 18. a third chamber opening; 19. an interface opening;

2. a light source; 21. a light emitting element; 22. a substrate; 23. a protection resistor; 24. a power supply interface;

3.a microlens array; 31. a microlens; 32. a plate body;

4. An optical fiber;

5. a porous plate; 51. a well;

6. A double cemented lens; 60. a lens barrel;

71. A first optical insert; 72. a second optical insert;

8. an optical fiber array panel; 81. an incident array; 82. an exit array; 83. an entry hole; 84. an exit aperture; 85. an annular support portion; 86. an annular limit part; 87. a groove; 88. an annular clamping groove; 89. a relief groove;

9. a mounting member; 91. a mounting groove; 92. a photographing opening; 93. an arc-shaped groove;

200. a smart phone; 201. a camera is provided.

Detailed Description

Embodiments of the present invention are described in further detail below with reference to the accompanying drawings and examples. The following examples are illustrative of the invention and are not intended to limit the scope of the invention.

In describing embodiments of the present invention, it should be noted that the terms "first" and "second" are used for clarity in describing the numbering of the product components and do not represent any substantial distinction unless explicitly stated or defined otherwise. "up", "down", "left", "right" and the like are used only to indicate a relative positional relationship, and when the absolute position of the object to be described is changed, the relative positional relationship may be changed accordingly. The specific meaning of the above terms in the embodiments of the present invention will be understood by those of ordinary skill in the art according to specific circumstances.

It should be noted that the term "coupled" is to be interpreted broadly, as being able to be coupled directly or indirectly via an intermediary, unless explicitly stated or defined otherwise. The specific meaning of the above terms in embodiments of the present invention will be understood in detail by those of ordinary skill in the art.

As shown in fig. 1 and fig. 2, the biochemical detection image acquisition system provided by the embodiment of the invention comprises a camera bellows 1, a light source 2, a micro lens array 3 and a plurality of optical fibers 4.

The device comprises a camera bellows 1, a first chamber 11 and a second chamber 12, wherein the first chamber 11 and the second chamber 12 are arranged in the camera bellows 1 at intervals along the direction perpendicular to the height, the first chamber 11 is a detection chamber of a sample of a detected object, the first chamber 11 is used for accommodating a porous plate 5 with a plurality of hole wells 51 arranged in an array, the porous plate 5 is a carrier tool for accommodating the sample of the detected object, one hole well 51 is used for accommodating one sample, the hole wells 51 can transmit light along the axial direction, and a comparison experiment of a plurality of groups of samples can be realized once by using the porous plate 5 so as to realize large-flux detection; the second chamber 12 is not communicated with the first chamber 11 along the direction perpendicular to the height of the camera bellows 1 so as to avoid the mutual influence of light rays in the first chamber 11 and the second chamber 12; a window (not shown) is arranged on the top surface of the camera bellows 1 opposite to the second chamber 12, and the window penetrates into the second chamber 12; the top of the camera box 1 can be detachably provided with a portable electronic device with a camera.

The light source 2 is arranged at the top in the first chamber 11 for emitting illumination light, and when the porous plate 5 is accommodated in the first chamber 11, the light source 2 is positioned above the porous plate 5.

The number of the optical fibers 4 is the same as that of the hole wells 51 on the porous plate 5, one end of each optical fiber 4 in the plurality of optical fibers 4 is fixed at the bottom in the first chamber 11, the end faces of the plurality of optical fibers 4 are arranged in a plane array at the bottom in the first chamber 11 to form an optical fiber input array at the bottom in the first chamber 11, the arrangement of the optical fiber input array is the same as that of the hole well 51 array, and when the porous plate 5 is received in the first chamber 11 and the hole well 51 array is aligned with the optical fiber input array, the centers of the plurality of hole wells 51 are aligned with the end face centers of the plurality of optical fibers 4 of the optical fiber input array one by one; while the other end of each optical fiber 4 of the plurality of optical fibers 4 is fixed to the bottom in the second chamber 12, the other end faces of the plurality of optical fibers 4 are also arranged in a planar array at the bottom in the second chamber 12 to form an optical fiber output array at the bottom in the second chamber 12.

The microlens array 3 is provided with a plurality of microlenses 31 arranged in an array, the number of the microlenses 31 is the same as that of the optical fibers 4, the arrangement of the microlens array 3 is the same as that of the optical fiber input array, the microlens array 3 is arranged at the bottom in the first cavity 11 and is positioned above the optical fiber input array, the microlens array 3 is aligned with the optical fiber input array, the centers of the plurality of microlenses 31 are aligned with the center of the end faces of the plurality of optical fibers 4 of the optical fiber input array one by one, and each microlens 31 is associated with one end face of one optical fiber 4; when the multi-well plate 5 is received in the first chamber 11 and the array of wells 51 is aligned with the fiber input array, the array of micro-lenses 3 is positioned below the multi-well plate 5, the array of wells 51 is also aligned with the array of micro-lenses 3, and the centers of the plurality of wells 51 are aligned one-to-one with the centers of the plurality of micro-lenses 31 such that each well 51 is associated with one micro-lens 31.

Thus, as shown in fig. 3, in the first chamber 11, the light source 2, the wells 51 of the porous plate 5, the microlenses 31 of the microlens array 3, and the end surfaces of the optical fibers 4 of the optical fiber input array are arranged in vertical center alignment; within the second chamber 12, the fiber optic output array is arranged in vertical central alignment with the camera 201 of the portable electronic device. One end of the optical fiber 4 is positioned at the bottom of the hole well 51 of the porous plate 5 to acquire optical information at the bottom of the hole well 51, and then the optical information (including fluorescence, color development and color change) is conducted to the emergent end of the optical fiber 4; the optical fiber 4 is a transmission tool of extremely fine light, the emergent end face of the optical fiber 4 can be converged to form a neat optical fiber output array, the shot view field of the optical fiber output array is obviously reduced compared with the plate face of the perforated plate 5, which is perpendicular to the hole well 51, the shooting imaging of portable electronic equipment is facilitated, the limitation of focusing distance is reduced, the volume of an optical structure is reduced, the volume of a biochemical detection image acquisition system is further reduced, and the portability is improved.

FIG. 4 is a schematic diagram of an optical path of the biochemical detection image capturing system according to the embodiment of the present invention when in operation; the solid arrows in fig. 4 indicate the propagation direction of the light rays. When the portable electronic device starts to work, the camera 201 of the portable electronic device is made to face the window; after light emitted by the light source 2 passes through the hole well 51 of the porous plate 5 filled with the sample, the micro lens 31 of the micro lens array 3 couples light beams into the optical fiber 4 positioned in the end face of the optical fiber input array, and the optical fiber 4 transmits light signals into the end face positioned in the optical fiber output array to form a luminous optical fiber output array; the outgoing light beams of the optical fiber output array are focused on the CMOS sensor by the camera 201 of the portable electronic device to form image information, so that image acquisition is realized.

The biochemical detection image acquisition system is used in combination with portable electronic equipment with a camera 201 to meet the requirement of multi-flux biochemical detection of an object to be detected; by arranging the optical fiber 4 array, the shot view field can be obviously reduced, shooting and imaging of portable electronic equipment are facilitated, the limit of focusing distance is reduced, the volume of an optical structure is reduced, the volume of a biochemical detection image acquisition system is further reduced, and portability is improved; by arranging the micro lens array 3 above the optical fiber input array, the micro lens 31 can collect all light emitted from the bottom of the hole well 51 into the input end face of the optical fiber 4, so that the light receiving efficiency of the optical fiber 4 is greatly improved, the intensity of light coupled into the optical fiber 4 is enhanced, the light coupling efficiency of the light is improved, the light intensity of the light beam output by the optical fiber 4 is improved, the image acquisition precision is improved, the detection sensitivity and accuracy are improved, and the quantitative measurement precision is further improved; the biochemical detection image acquisition system can acquire quantitative detection images with large flux and high precision, has a simple structure, is convenient to carry and low in cost, can meet the requirements of laboratories, hospitals and the like on biochemical detection, and can be widely popularized and applied in the fields of medical diagnosis, food safety, environmental supervision and the like.

Specifically, in the embodiment of the present invention, the portable electronic device may be a mobile phone, a tablet computer, a digital camera, or the like.

More specifically, as shown in fig. 2, in this embodiment, the portable electronic device is a smartphone 200, and the smartphone 200 has excellent image capturing capability and data processing capability. The smart phone 200 is provided with the recording software, after the smart phone 200 captures the image of the optical fiber output array, the recording software performs image processing and data analysis to realize the calibration test of the sample, and the image and the data are processed in real time on the biochemical detection site, so that the smart phone can be smoothly used in the environment with undeveloped network and can be applied to wider scenes.

Specifically, as shown in fig. 5, in this embodiment, the microlens array 3 further includes a plate body 32 having an overall rectangular shape, and a plurality of circular microlens 31 arrays are disposed on the plate body 32. The diameter of the microlens 31 is smaller than the diameter of the well 51 of the porous plate 5.

Specifically, in the embodiment of the present invention, the optical fiber 4 is a multimode optical fiber with a diameter of 600nm, and the transmission light characteristic is that the wavelength is between 400nm and 700nm, so as to transmit visible light.

Further, as shown in fig. 2, in the embodiment of the present invention, the biochemical detection image capturing system further includes a porous plate 5 detachably disposed in the first chamber 11, the porous plate 5 is located between the light source 2 and the microlens array 3, and the centers of the plurality of hole wells 51 are aligned with the centers of the plurality of microlenses 31 one by one.

Specifically, as shown in fig. 6, in this embodiment, the multi-well plate 5 is a 96-well plate having a total of 8×12 96 wells 51. Then the number of the optical fibers 4 is 96 in a matched way, and the end faces of the optical fibers 4 of the optical fiber output array are distributed in an 8 multiplied by 12 array; the microlens array 3 has 96 microlenses 31 in total of 8×12.

In addition, in an embodiment not shown, the multi-well plate 5 may also have more or fewer well wells 51, for example the multi-well plate 5 is a 24-well plate, a 48-well plate, a 384-well plate, or the like.

Specifically, as shown in fig. 6, in this embodiment, the cross-sectional area of the fiber output array is 1/100 of the cross-sectional area of the porous plate 5. The cross section of the fiber output array is a cross section perpendicular to the length direction of the optical fibers 4, and the cross section of the porous plate 5 is a cross section perpendicular to the axial direction of the well 51. The shot view field (namely the optical fiber output array) is larger than the reduction ratio of the cross section area of the perforated plate 5, so that the risk of edge distortion can be reduced when the portable electronic equipment is used for shooting, the image acquisition precision is improved, the imaging object distance can be reduced, the volume of the biochemical detection image acquisition system is further reduced, and the portability is improved.

Further, as shown in fig. 7, in the embodiment of the present invention, the light source 2 includes a plurality of light emitting elements 21 arranged in an array, the number of the light emitting elements 21 is the same as the number of the microlenses 31, and the arrangement of the array of the light emitting elements 21 is the same as the arrangement of the microlens array 3, and the centers of the plurality of light emitting elements 21 are aligned one by one with the centers of the plurality of microlenses 31. Through setting up the luminescent element 21 the same with microlens 31 quantity, namely luminescent element 21's quantity is the same with the quantity of the hole well 51 of perforated plate 5, and luminescent element 21 array aligns with microlens array 3, namely luminescent element 21 array aligns with the hole well 51 array of perforated plate 5, every luminescent element 21 is located a hole well 51 center directly over, every luminescent element 21 is alone to a hole well 51 illumination, single hole well 51 can receive all light that single luminescent element 21 sent, reduce the light intensity loss, improve the light utilization ratio, and then the luminous intensity that optic fibre 4 received is higher, further improve the luminous intensity of optic fibre 4 exit end, promote the image acquisition precision, and then improve the detection sensitivity, be favorable to carrying out high-accuracy ration detection.

Specifically, as shown in fig. 7, in the embodiment of the present invention, the light emitting element 21 is an LED (LIGHT EMITTING Diode) lamp bead, the light source 2 further includes a substrate 22, and a plurality of LED lamp bead arrays are disposed on the substrate 22 to form an LED area array. The LED lamp beads can be selected from white lamp beads, blue lamp beads, red lamp beads, green lamp beads and the like.

More specifically, as shown in fig. 7, in this embodiment, the light source 2 further includes a protection resistor 23, where the protection resistor 23 is disposed on the substrate 22 and electrically connected to the LED lamp beads, and the protection resistor 23 is used to protect the LED lamp beads from voltage stabilization.

More specifically, as shown in fig. 7, in this embodiment, the light source 2 further includes a power supply interface 24, where the power supply interface 24 is used to be electrically connected with an external power supply, and the light source 2 can use an external power supply to supply power, so that a battery is not required, and the occupied space of the device is reduced, which is beneficial to reducing the volume of the biochemical detection image acquisition system, reducing the weight, and improving the portability. The power interface 24 may be a USB (Universal Serial Bus ) interface, such as Type-C (USB Type-C, a Type of interface on a physical level).

Specifically, as shown in fig. 2, in the embodiment of the present invention, at least two opposite inner side walls perpendicular to the top surface in the first chamber 11 are provided with first supporting portions 111, and the light source 2 is supported on the first supporting portions 111 and detachably connected to the first supporting portions 111. Through setting up the first supporting part 111 that is used for fixed light source 2 in first cavity 11, can with light source 2 demountable installation in first cavity 11, convenient maintenance replacement light source 2, increase of service life can be according to the perforated plate 5 that uses in addition correspondingly change light source 2, can also select and change light source 2 according to the sample type that detects, the selectivity of light source 2 is strong, reinforcing service function, need not to customize camera bellows 1 again, application scope is wider, reduce cost, the practicality is strong.

More specifically, in this embodiment, the first supporting portion 111 is a rib or a boss protruding from opposite inner side walls of the first chamber 11; the two sides of the substrate 22 of the light source 2 are supported on the convex ribs or bosses on the two sides of the first chamber 11 and are fixedly connected with the convex ribs or bosses through screws.

Specifically, as shown in fig. 2, in this embodiment, the side wall of the camera bellows 1 adjacent to the first supporting portion 111 is further provided with an interface opening 19, and when the light source 2 is supported on the first supporting portion 111, the power supply interface 24 is embedded and installed in the interface opening 19 and is exposed from the outer side wall of the camera bellows 1, so that the power supply interface 24 is electrically connected to an external power supply.

Further, as shown in fig. 2,3 and 4, in the embodiment of the present invention, the biochemical detection image acquisition system further includes a double cemented lens 6, and the double cemented lens 6 is disposed in the optical path between the optical fiber output array and the window; the center of the doublet 6 is aligned with the center of the fiber output array. The double-cemented lens 6 can effectively eliminate distortion, shortens the imaging distance on the basis of ensuring excellent imaging, and overcomes the problems of imaging edge distortion and large object distance requirement, so that the required height of the second chamber 12 can be reduced, the whole volume of the biochemical detection image acquisition system is further reduced, and the portability is improved.

Further, as shown in fig. 2, in this embodiment, the biochemical detection image capturing system further includes a lens barrel 60, the lens barrel 60 is disposed in the second chamber 12 and fixedly connected to the inner wall of the top surface of the camera bellows 1 around the window, and the double cemented lens 6 is disposed in the lens barrel 60. The lens cone 60 is used for fixedly mounting the double-cemented lens 6 in the second cavity 12 and aligning with the window, meanwhile, the light shielding performance and the sealing performance of the lens cone 60 are good, light leakage and light loss can be prevented, the image acquisition precision is ensured, and the requirement for accurate quantitative measurement is met.

Specifically, in this embodiment, the diameter of the doublet 6 is 25.4mm and the focal length is 25mm; the doublet 6 is fixed in the barrel 60 using a snap ring.

Specifically, in the embodiment of the invention, the image information captured by the portable electronic equipment through the biochemical detection image acquisition system can be combined with colorimetric detection, and is widely applied to the field of portable biochemical detection. Wherein, colorimetric detection is a common biochemical sensing detection means, and is calibrated by analyzing the colorimetric and chromogenic effects of the detected object.

Further, as shown in fig. 2,3 and 4, in the embodiment of the present invention, the biochemical detection image acquisition system further includes a first optical insert 71, and the first optical insert 71 is detachably disposed in the optical path between the light source 2 and the porous plate 5. The first optical insert 71 may be a light homogenizing sheet, for example ground glass, where the light homogenizing sheet can convert light emitted by the light source 2 into uniform light, and the wavelength of the uniform light is not changed, and directly irradiates the sample in the porous plate 5 to perform colorimetric detection; the first optical insert 71 may also be an optical filter, which can convert the light emitted by the light source 2 into light with a specific wavelength for colorimetric detection. Through setting up first optical insert 71, and first optical insert 71 can dismantle, through changing different grade type's first optical insert 71, can realize multiple different colorimetry and detect, satisfy the biochemical detection needs to multiple sample.

Specifically, as shown in fig. 2, in the embodiment of the present invention, at least two opposite inner side walls perpendicular to the top surface in the first chamber 11 are further provided with a second supporting portion 112, and the first optical insert 71 is supported on the second supporting portion 112 and detachably connected to the second supporting portion 112; wherein the second supporting portion 112 is located below the first supporting portion 111 to position the first optical insert 71 below the light source 2; when the porous plate 5 is received in the first chamber 11, the second support 112 is located at the upper side of the porous plate 5 to dispose the first optical insert 71 above the porous plate 5. By arranging the second supporting part 112 in the first chamber 11, the first optical inserting sheet 71 can be detachably arranged and fixed in the first chamber 11, and the first optical inserting sheet 71 is convenient and quick to replace.

More specifically, in this embodiment, the second supporting portion 112 is a rib or a boss protruding from two opposite inner side walls of the first chamber 11; the ground glass or the optical filter is supported on the ribs or bosses on the two sides of the first chamber 11 on the two sides and is fixed on the ribs or bosses by screws.

Further, as shown in fig. 2, 3 and 4, in the embodiment of the present invention, the biochemical detection image capturing system further includes a second optical insert 72, where the second optical insert 72 is detachably disposed in the optical path between the porous plate 5 and the microlens array 3, and the second optical insert 72 is an optical filter. Fluorescence detection is also a common biochemical sensing detection means, and is calibrated by analyzing colorimetric, chromogenic and fluorescent effects of the detected object. The difference between fluorescence detection and colorimetric detection is that in the fluorescence method, light needs to pass through an emission filter after passing through a sample of an object to be detected to obtain fluorescence with a certain wavelength. By arranging the optical filter for fluorescence emission in the optical path between the porous plate 5 and the micro lens array 3, the image information captured by the portable electronic equipment through the biochemical detection image acquisition system of the embodiment of the invention can be combined with fluorescence detection, so that the fluorescence detection can be realized, and the portable electronic equipment can be widely applied to the field of portable biochemical detection; and the second optical insert 72 is detachable, the second optical insert 72 is combined with the first optical insert 71, the color comparison and fluorescence detection modes can be switched by flexibly assembling the optical filter for excitation and emission, the biochemical sensing detection of the color comparison and fluorescence modes is realized, the use function is enhanced, and the biochemical detection image acquisition system has the advantages of double modes and portability, can adapt to the detection requirements of various scenes, is suitable for the detection of various samples, and is efficient and environment-friendly.

Specifically, in the embodiment of the present invention, when the selection mode is a colorimetric detection method, a first optical insert 71 is inserted into the optical path between the light source 2 and the porous plate 5, and the first optical insert 71 is a light homogenizing sheet or a light filter; when the selection mode is the fluorescence detection method, a first optical insert 71 is inserted into the optical path between the light source 2 and the porous plate 5, a second optical insert 72 is inserted into the optical path between the porous plate 5 and the microlens array 3, the first optical insert 71 is a first optical filter, the first optical filter is used as an excitation filter, and the second optical insert 72 is a second optical filter, and the second optical insert 72 is used as an emission filter.

More specifically, in this embodiment, the light homogenizing sheet is ground glass.

Further, as shown in fig. 2, in the embodiment of the present invention, a third chamber 13 is further disposed inside the dark box 1, the third chamber 13 is located below the first chamber 11 and the second chamber 12, and the third chamber 13 is in communication with the first chamber 11 and the second chamber 12, and the plurality of optical fibers 4 are all accommodated in the third chamber 13. Through setting up third cavity 13, light-shielding property and closure are good, prevent light leak and light intensity loss, satisfy the demand to accurate quantitative measurement.

Further, as shown in fig. 8, in the embodiment of the present invention, the biochemical detection image acquisition system further includes an optical fiber array panel 8, the optical fiber array panel 8 includes an incident array 81 and an exit array 82, the incident array 81 has a plurality of incident holes 83 arranged in an array, the number of the incident holes 83 is the same as that of the optical fibers 4, and the arrangement of the array of the incident holes 83 is the same as that of the microlens array 3; the exit array 82 has a plurality of exit holes 84 arranged in an array, and the number of exit holes 84 is the same as the number of optical fibers 4. As shown in fig. 2, the optical fiber array panel 8 is disposed on top of the third chamber 13 and closes the bottoms of the first chamber 11 and the second chamber 12, such that the incident array 81 is located at the bottom in the first chamber 11, the exit array 82 is located at the bottom in the second chamber 12, and the centers of the plurality of incident holes 83 are aligned with the centers of the plurality of microlenses 31 one by one, and the center of the exit array 82 is aligned with the center of the window; one end of each optical fiber 4 in the plurality of optical fibers 4 is fixedly connected to the incident hole 83, and the other end is fixedly connected to the emergent hole 84, so that two end surfaces of the plurality of optical fibers 4 are respectively arranged to form an optical fiber input array and an optical fiber output array. The optical fiber array panel 8 is arranged to realize the installation and fixation of a plurality of optical fibers 4, so that the structure is simple, the disassembly and assembly are quick and convenient, the position stability of the optical fiber input array and the optical fiber output array is ensured, and the image acquisition precision is ensured.

Specifically, as shown in fig. 8, in this embodiment, an annular support portion 85 is provided on the optical fiber array panel 8 around the incident array 81, and the annular support portion 85 is used to support the microlens array 3 so that the microlens array 3 is spaced apart from the incident array 81 by a certain distance, and also to facilitate the microlens 31 to collect light on the end face of the optical fiber 4. For example, the distance between the center of the microlens 31 and the center of the incident hole 83 is equal to the focal length of the microlens 31.

Specifically, as shown in fig. 8, in this embodiment, an annular limiting portion 86 is further disposed on the optical fiber array panel 8 around the annular supporting portion 85, the annular limiting portion 86 encloses a limiting groove, the microlens array 3 is embedded in the limiting groove, the annular limiting portion 86 limits the relative positions of the microlens array 3 and the incident array 81, so that the centers of the microlenses 31 and the centers of the incident holes 83 are aligned one by one, and measurement accuracy is ensured.

More specifically, as shown in fig. 8, in this embodiment, a groove 87 is concavely formed on the plate surface of the optical fiber array panel 8, and an array of incidence holes 83 of the incidence array 81 is provided in the groove 87; the plate surface of the fiber array panel 8 around the outer periphery of the groove 87 forms the annular support portion 85.

More specifically, as shown in fig. 2, in this embodiment, the second optical insert sheet 72 is embedded in a limit groove surrounded by an annular limit portion 86 on the fiber array panel 8 and is located above the microlens array 3.

Specifically, as shown in fig. 8, in this embodiment, an annular clamping groove 88 is further provided on the optical fiber array panel 8 around the outer periphery of the annular limiting portion 86, a clamping portion is provided at the bottom of the porous plate 5, and the annular clamping groove 88 is used for matching and clamping with the clamping portion at the bottom of the porous plate 5 so as to fixedly push the porous plate 5 in the first chamber 11. For example, the annular clamping groove 88 is rectangular.

More specifically, in this embodiment, the fiber array panel 8 is a stainless steel plate, and the fiber array panel 8 is fixed on top of the third chamber 13 by screws.

Specifically, as shown in fig. 2, in the embodiment of the present invention, the camera bellows 1 includes a housing 14 and a magnetic side door 15, a first chamber 11, a second chamber 12 and a third chamber 13 are formed in the housing 14, a side surface of the housing 14 perpendicular to the top surface thereof is opposite to the first chamber 11 and is provided with a strip-shaped opening 16, the strip-shaped opening 16 forms a strip-shaped channel communicating with the first chamber 11, and the strip-shaped opening 16 is used for detachably mounting the porous plate 5 in the first chamber 11; the magnetic side door 15 is magnetically connected with the box 14 and closes the strip-shaped opening 16. As shown in fig. 9, when the magnet side door 15 is removed, the first chamber 11 is in an open state, and the porous plate 5 is inserted into the first chamber 11 or taken out from the first chamber 11 through the strip-shaped opening 16; when closing magnetic side door 15, first cavity 11 is in the closed state, can carry out formal testing process this moment, and light-shielding nature and closure are good, prevent light leak and light loss, satisfy the demand to accurate quantitative measurement.

Specifically, in this embodiment, the back surface of the magnetic side door 15 is provided with a strong magnetic patch, and the magnetic side door 15 can be attracted to the side wall of the case 14 around the strip-shaped opening 16 by the strong magnetic patch, to realize opening and closing of the first chamber 11.

More specifically, as shown in fig. 9, in this embodiment, the second support portion 112 is provided with a magnetic attraction piece 113 toward the outer edge of the strip-shaped opening 16, the magnetic attraction piece 113 may be a strong magnetic patch, and the strong magnetic patch on the back of the magnetic attraction side door 15 is magnetically attracted and mated with the magnetic attraction piece 113, so that the magnetic attraction side door 15 is magnetically attracted and connected with the case 14.

Specifically, as shown in fig. 8 and 9, in this embodiment, the side edge of the fiber array panel 8 adjacent to the strip-shaped opening 16 is provided with a relief groove 89, for example, the relief groove 89 is a trapezoidal groove; the relief groove 89 is used for avoiding the clamping tool, and the clamping tool can place and take the porous plate 5 at the relief groove 89.

Further, as shown in fig. 2, in the embodiment of the present invention, the camera bellows 1 further includes a magnetic baffle 17, and at least one side surface of the box 14 perpendicular to the top surface thereof is opposite to the third chamber 13 and provided with a third chamber opening 18 communicating with the third chamber 13; the magnetic attraction baffle 17 is magnetically attracted to the case 14 and closes the third chamber opening 18. By providing the third chamber opening 18, when the magnetic shield 17 is removed, the third chamber 13 is opened, and at this time, the position of the optical fiber 4 can be checked and adjusted; when the magnetic attraction baffle 17 is installed, the third chamber 13 is in a closed state, and at the moment, a formal test process can be carried out, so that the light shielding performance and the sealing performance are good, light leakage and light loss are prevented, and the requirement for accurate quantitative measurement is met.

Specifically, as shown in fig. 2, in this embodiment, the camera bellows 1 includes two magnetic attraction baffles 17, and the opposite sides of the housing 14 are provided with third chamber openings 18.

More specifically, in this embodiment, the back of the magnetic attraction baffle 17 is provided with a strong magnetic patch, the magnetic attraction baffle 17 can be temporarily attracted to the side wall of the box 14 through the strong magnetic patch in the pre-test and adjustment stage, and the magnetic attraction baffle 17 is fastened and connected with the box 14 through screws after the position of the optical fiber 4 bundle is confirmed without errors.

In a specific embodiment, as shown in fig. 2, the case 14 includes an upper case 141, a lower case 142, and a top cover 143, and two cavities penetrating in the height direction are formed in the upper case 141, and the two cavities are separated by a partition plate, so that light is prevented from being affected; the side wall of one cavity far away from the other cavity is provided with a strip-shaped through hole, namely a strip-shaped opening 16; a cavity with an open top is formed in the lower box body 142, and through holes, namely a third cavity opening 18, are formed in two opposite side walls of the lower box body 142; the top cover 143 is also provided with a through hole, namely a window; the upper case 141 is fixedly connected to the top of the lower case 142 by a screw, and the top cover 143 is fixedly connected to the top of the upper case 141 by a screw and closes the top opening of the cavity of the upper case 141; so that the top cover 143 and the cavity of the upper case 141 with the bar-shaped opening 16 enclose a first chamber 11, the top cover 143 and the other cavity of the upper case 141 enclose a second chamber 12, and the cavity in the lower case 142 forms a third chamber 13.

Further, as shown in fig. 1 and 2, in the embodiment of the present invention, the biochemical detection image acquisition system further includes a mounting member 9, where the mounting member 9 is detachably connected to the top of the camera box 1, and the mounting member 9 is used for fixedly mounting the portable electronic device, and making the camera 201 of the portable electronic device face the window. Through setting up detachable mounting piece 9, mounting piece 9 can set up multiple specification, and the biochemical detection image acquisition system of this invention not only can use with the portable electronic equipment of current multiple model, can also use with other new portable electronic equipment combination, if change portable electronic equipment that detects usefulness, need not to customize again or change whole camera bellows 1, only need customize new mounting piece 9 can, reduce cost, the suitability is stronger, the application scene is extensive.

Specifically, as shown in fig. 2, in this embodiment, the mount 9 is provided with a mounting groove 91, the shape and size of the mounting groove 91 being adapted to the shape and size of the portable electronic device, the mounting groove 91 being used for mounting the portable electronic device; the bottom of the mounting groove 91 is provided with a camera opening 92 facing the window, and the camera opening 92 is used for exposing a camera 201 of the portable electronic device. The mounting piece 9 is customized according to the used portable electronic equipment, the adaptability is better, the size of the mounting groove 91 is strictly the same as that of the portable electronic equipment, the portable electronic equipment is firmly and stably mounted, and the image acquisition precision is ensured.

Specifically, as shown in fig. 2, in this embodiment, the side edge of the mounting groove 91 is further provided with an arc groove 93, and the arc groove 93 is used for placing and taking the portable electronic device, which is convenient for operation.

More specifically, in this embodiment, the mounting member 9 is a mounting plate, the mounting member 9 is fixedly connected to the top cover 143 on the top of the camera box 1 by screws, and the image pickup opening 92 of the mounting member 9 is concentrically aligned with the window on the top surface of the camera box 1.

The biochemical detection image acquisition system of the present invention is specifically described below by taking a biochemical detection image acquisition system which is used in combination with the smart phone 200, is applicable to a 96-well plate, and has a colorimetric and fluorescent dual mode as an example.

In the embodiment, the whole size of the camera bellows 1 is 200mm multiplied by 100mm multiplied by 150mm, and the camera bellows is small in size and good in portability. The 96-well plate has a total of 96 wells 51 of 8×12. The total number of optical fibers 4 is 96.

The light source 2 is an LED area array, and is provided with 96 LED lamp beads 8 multiplied by 12, a1 omega protection resistor 23 and a Type-C power supply interface 24, wherein the parameters of each LED lamp bead are as follows: rated voltage 3V, rated current 0.02A, total illumination power 5.76W; the light source 2 is connected into the power supply system through a Type-C power supply line with a switch.

The microlens array 3 has 96 microlenses 31 in total of 8×12, the diameter of the microlenses 31 is not more than 7mm, the focal length is 0.5mm, the lens thickness is 0.6mm, and the pitch of the microlenses 31 is 9mm.

The diameter of the doublet 6 is 25.4mm and the focal length is 25mm.

The size of the incidence array 81 of the optical fiber array panel 8 is 110mm×72mm, 96 incidence holes 83 of 8×12 are arranged, the incidence holes 83 are through holes with the diameter of 0.6mm, and the interval between the incidence holes 83 is 9mm; the size of the exit array 82 was 12.8mm×8.55mm, and a total of 8×12 96 exit holes 84 were provided, the exit holes 84 were through holes having a diameter of 0.6mm, and the exit holes 84 were spaced apart by 0.9mm. The area of the exit array 82 is 1/100 of the cross-sectional area of the 96-well plate.

The rear camera 201 of the smartphone 200 has more than 1000 ten thousand pixels, and the screen resolution is not lower than 1080P (1920×1080). The smart phone 200 is provided with colorimetric and fluorescent dual-mode sensing detection recording software.

As shown in fig. 3, inside the dark box 1, 96 lamp beads of the LED area array, 96 wells 51 of the 96-well plate, 96 microlenses 31 of the microlens array 3, and 96 entrance holes 83 of the incident array 81 of the fiber array panel 8 are arranged in vertical center alignment; the emergent array 82 of the optical fiber array panel 8 is vertically and centrally aligned with the double-cemented lens 6 and the camera 201 of the smart phone 200.

When a colorimetric method is adopted, frosted glass or an optical filter is arranged between the LED area array and the 96-well plate, when the frosted glass is arranged, light emitted by the LED area array is converted into uniform light, the wavelength is not changed, and a sample is directly irradiated for detection; when the optical filter is arranged, light emitted by the LED area array is converted into light with specific wavelength for colorimetric detection. The size of the frosted glass and the filter is 130mm multiplied by 88mm, and the thickness is not more than 1.5mm.

When a fluorescence method is adopted, a first filter is arranged between the LED area array and the 96-pore plate and used as an excitation filter, the size of the first filter is 130mm multiplied by 88mm, and the thickness of the first filter is not more than 1.5mm; a second filter is arranged between the 96-well plate and the micro lens array 3 and used as an emission filter, the size of the second filter is 110mm multiplied by 72mm, and the thickness of the second filter is not more than 1.5mm.

As shown in fig. 10, based on the biochemical detection image acquisition system provided in the above embodiment, the embodiment of the present invention further provides a biochemical detection image acquisition method, which specifically includes the following steps:

S101, determining parameters of the light source 2 according to the characteristics of the detected object and biochemical detection experiment requirements. The light source 2 is selected according to the determined parameters of the light source 2, and the light source 2 is fixedly installed in the first chamber 11 of the camera bellows 1 through the first supporting part 111.

S102, determining a test mode as a colorimetric method or a fluorescent method according to the characteristics of the detected object and the biochemical detection experiment requirements, and determining parameters of the first optical insert 71 and the second optical insert 72 according to the test mode.

If a fluorescence method is selected, an excitation filter is fixed in the first chamber 11 of the camera bellows 1 through the second supporting part 112, an emission filter is embedded in the optical fiber array panel 8, and parameters of the excitation filter and the emission filter are determined. If a colorimetry is selected, judging whether the illumination is required by the light with the specific wavelength, and if the illumination is not required by the light with the specific wavelength, fixing ground glass at the second supporting part 112 in the first chamber 11 of the camera bellows 1; if light of a specific wavelength is required, the filter is fixed in the first chamber 11 of the camera bellows 1 by the second support 112, and parameters of the filter are determined.

S103, preparing a sample of the object to be measured, and storing the sample of the object to be measured in the plurality of wells 51 of the porous plate 5.

S104, confirming that the light source 2 is in a closed state, feeding the porous plate 5 into the first chamber 11, and confirming that the camera bellows 1 is in a closed state.

Before the beginning of the official experiment, it was necessary to determine that the light source 2 was in the off state. Opening the magnetic side door 15, clamping the side edge of the porous plate 5 by using tweezers, stably feeding the porous plate 5 into the first chamber 11, and taking out the tweezers after the bottom of the porous plate 5 stably falls into the annular clamping groove 88 on the optical fiber array panel 8; after the porous plate 5 is placed, the biochemical detection image acquisition system is shown in fig. 9. And then the magnetic side door 15 and the magnetic baffle 17 are magnetically connected to the box body 14, and the strip-shaped opening 16 and the third chamber opening 18 are closed, so that the inside of the camera bellows 1 is fully closed.

S105, the portable electronic device having the camera 201 is mounted on the top of the camera case 1, the camera 201 is faced to the window, and the camera 201 is opened.

And opening a photographing mode of corresponding recording software in the smart phone 200, and entering a standby working mode.

S106, turning on the light source 2, performing focusing operation of the camera 201 on the portable electronic device, and performing photographing operation after the camera 201 is focused successfully to obtain a biochemical detection image.

The switch on the power supply line is turned on to turn on all the LED lamp beads of the light source 2, at this time, as shown in fig. 4, the light emitted by the LED lamp bead area array is converted into uniform light or light with a special wavelength after passing through the frosted glass or the optical filter, after the light beam passes through the porous plate 5 filled with the sample (colorimetric solution) of the object to be measured, the micro lens array 3 couples the light beam into one end of the multimode optical fiber 4, and the optical fiber 4 transmits the light signal to the other end to form a luminous exit array 82; focusing operation is performed on the smart phone 200, when focusing is successful, a shutter is pressed down, photographing work is completed, and outgoing light beams are focused on the CMOS sensor by the double-cemented lens 6 and the camera 201 of the smart phone 200 to form image information, so that a biochemical detection image is obtained.

S107, the LED lamp beads of the light source 2 are turned off, the porous plate 5 is taken out by using the forceps clamp, and the first chamber 11 of the camera bellows 1 is restored to a closed state.

Finally, on the smart phone 200, image and data processing is performed through the carried recording software, and the content calibration of the measured object is obtained by combining the calibration curve, so that the calibration test of the sample is realized.

The biochemical detection image acquisition system provided by the embodiment of the invention is simple and convenient to use, and has the advantages of large flux, double modes, high precision, portability and wide application scene.

In a specific embodiment, when a colorimetric method is selected to detect pyrophosphate, the light-emitting element 21 in the light source 2 is a white LED with a spectral range of 400nm-700nm, the first optical insert 71 is ground glass, and after imaging by the camera 201 of the smart phone 200, RGB three-channel color analysis is performed, and content calibration is performed in combination with a calibration curve.

When a colorimetry is selected to detect pathogenic bacteria, the light-emitting element 21 in the light source 2 is a blue light LED with the spectral range of 464nm, the first optical insert 71 is ground glass, the RGB three-channel color information is captured after the imaging of the camera 201 of the smart phone 200 and converted into gray values, and the content calibration is carried out by combining a calibration curve.

When a colorimetric method is selected for detecting protein, a white light LED with the spectral range of 400-700 nm is selected as the light-emitting element 21 in the light source 2, a narrow-band filter is selected as the first optical insert 71, the parameters are 615nm, RGB three-channel color information is captured after the imaging of the camera 201 of the smart phone 200, and the content calibration is carried out in combination with a calibration curve.

When the fluorescence method is selected to detect nucleic acid, the light-emitting element 21 in the light source 2 is a white light LED with the spectral range of 400nm-700nm, the first optical insert 71 is an excitation filter with the parameter of 465nm, the second optical insert 72 is an emission filter with the parameter of 530nm, the camera 201 of the smart phone 200 captures RGB three-channel color information after imaging, and only the green channel information is used and the content calibration is carried out by combining a calibration curve.

While the invention has been described in detail in the foregoing general description and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.

Claims

1. A biochemical test image acquisition system, comprising:

a light source disposed at a top portion within the first chamber and including a plurality of light emitting elements;

The micro lens array is provided with a plurality of micro lenses arranged in an array manner, the micro lens array is arranged in the first cavity and is positioned above the optical fiber input array, the centers of the micro lenses are aligned with the end face centers of the optical fibers of the optical fiber input array one by one, and the centers of the light emitting elements are aligned with the centers of the micro lenses one by one;

The biochemical detection image acquisition system further comprises a first optical insertion sheet, wherein the first optical insertion sheet is detachably arranged in a light path between the light source and the porous plate;

the biochemical detection image acquisition system further comprises a second optical insertion sheet, wherein the second optical insertion sheet is detachably arranged in a light path between the porous plate and the micro lens array;

The cross-sectional area of the fiber output array is 1/100 of the cross-sectional area of the porous plate;

The biochemical detection image acquisition system further comprises an optical fiber array panel, wherein the optical fiber array panel comprises an incident array and an emergent array, the incident array is provided with a plurality of incident holes arranged in an array, the arrangement of the incident holes is the same as that of the hole wells on the porous plate, and the emergent array is provided with a plurality of emergent holes arranged in an array; the optical fiber array panel is arranged at the bottoms of the first cavity and the second cavity, the incident array is positioned in the first cavity, the emergent array is positioned in the second cavity, and the centers of the plurality of incident holes are aligned with the centers of the plurality of microlenses one by one; one end of each optical fiber in the plurality of optical fibers is fixedly connected with the entrance hole, and the other end is fixedly connected with the exit hole;

An annular supporting part is arranged on the optical fiber array panel around the incidence array and is used for supporting the micro lens array;

The biochemical detection image acquisition system further comprises a double-cemented lens, wherein the double-cemented lens is arranged in a light path between the optical fiber output array and the window, and the center of the double-cemented lens is aligned with the center of the optical fiber output array.

2. The biochemical-detection image capturing system according to claim 1, wherein,

The optical fiber array panel is further provided with an annular clamping groove around the annular supporting portion, and the annular clamping groove is used for being matched and clamped with the bottom of the porous plate.

3. The biochemical-detection image acquisition system according to any one of claims 1 to 2, wherein,

The first chamber is internally provided with first supporting parts on at least two opposite inner side walls perpendicular to the top surface, and the light source is supported on the first supporting parts and detachably connected with the first supporting parts.

4. The biochemical-detected image acquisition system according to claim 3, wherein,

The light source further comprises a power supply interface, an interface opening is further formed in the side wall, adjacent to the first supporting portion, of the camera bellows, and the power supply interface is embedded and installed in the interface opening and is exposed out of the outer side wall of the camera bellows.

5. The biochemical-detection image capturing system according to claim 1, wherein,

And second supporting parts are further arranged on two opposite inner side walls, perpendicular to the top surface, of the first cavity, and the first optical inserting sheet is supported on the second supporting parts and detachably connected with the second supporting parts.

6. A biochemical test image acquisition method, characterized in that the biochemical test image acquisition system according to claim 1 is applied, comprising the steps of: