CN217237745U - Miniature multichannel fluorescence detection system - Google Patents
Miniature multichannel fluorescence detection system Download PDFInfo
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- CN217237745U CN217237745U CN202220237452.1U CN202220237452U CN217237745U CN 217237745 U CN217237745 U CN 217237745U CN 202220237452 U CN202220237452 U CN 202220237452U CN 217237745 U CN217237745 U CN 217237745U
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Abstract
The utility model belongs to the technical field of fluorescence detecting system, concretely relates to miniature multichannel fluorescence detecting system, this miniature multichannel fluorescence detecting system includes: the system comprises an LED excitation device, a PD receiving device and a sample collection platform; the corresponding LED light-emitting mechanism in the LED excitation device emits light with corresponding wave bands, the light irradiates on a sample of the sample collection platform through the emergent lens group and the emergent cylindrical lens in sequence, and the measured light irradiates on each PD photoelectric collection mechanism in the PD receiving device through the emergent cylindrical lens and the emergent lens group in sequence so as to detect the measured light; the utility model discloses the excitation light irradiation that can the corresponding wave band of directional selection output is on the sample, and receive the photometry that is surveyed that the sample sent and obtain the testing result, can satisfy the fluorescence detection demand of different wave bands, compact structure, simplification light path design simultaneously reduce optical element's quantity and cost, have reduced the optical system volume to use the scanning structure of fixed light path design replacement motion, make fluorescence repeatability higher.
Description
Technical Field
The utility model belongs to the technical field of fluorescence detecting system, concretely relates to miniature multichannel fluorescence detecting system.
Background
The fluorescence detection is a spontaneous luminescence reaction, and can detect human cells, bacteria, mould and food residues through the reaction of luciferase and ATP, and a reaction result can be obtained within 15 seconds. Illuminance, measured by a dedicated device and expressed in digital form, was first applied to the food industry in 1975 and was applied to the cosmetic manufacturing industry in 1985. The main drawback of fluorescence detection is that only a few compounds produce fluorescence. Most molecules do not fluoresce but contain derivatizable functional groups for synthesis of derivatives that fluoresce, for example, ortho-phthalaldehyde is a commonly used fluorophore for post-column derivatization of amino acids. Although fluorescence detection is very sensitive, such high sensitivity is not required for common sample analysis. Because the response of ELSDs is independent of fluorophores, no derivatization is required, thus greatly reducing sample pretreatment and analysis time.
The fluorescence detection means that present is used in on QPCR appearance generally adopts one end to launch exciting light excitation reagent, and the fluorescence that launches in the other end receiving reagent to reach the fluorescence detection of a wave band, if there is the detection demand of a plurality of wave bands, then use the similar optical structure of multiple wave bands, then constitute scanning structure, the same object of asynchronous scanning is removed to different wave bands, overall structure is bigger than normal, has the motion structure in the equipment, and optical system stability is not enough.
Therefore, it is necessary to develop a new micro multi-channel fluorescence detection system to solve the above problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a miniature multichannel fluorescence detecting system to solve the problem of how to satisfy the fluorescence detection demand of different wave bands.
In order to solve the technical problem, the utility model provides a miniature multichannel fluorescence detecting system, it includes: the device comprises an LED excitation device, a PD receiving device and a sample collection platform; the LED excitation device is provided with a plurality of LED light-emitting mechanisms, and the corresponding LED light-emitting mechanisms emit light with corresponding wave bands, and the light irradiates on a sample of the sample collection platform through the ejection lens group and the ejection cylindrical lens in sequence, so that the sample is excited to be measured, namely the measured light irradiates on each PD photoelectric collection mechanism in the PD receiving device through the ejection cylindrical lens and the ejection lens group in sequence, and the measured light is detected.
Further, the LED excitation device includes: the LED illuminating device comprises an LED excitation box, a plurality of LED light-emitting mechanisms, an emergent lens group and an emergent cylindrical lens; an excitation installation cavity is arranged in the LED excitation box, and the LED light-emitting mechanisms, the emergent lens group and the emergent cylindrical lens are arranged in the excitation installation cavity; the emission lens group is internally provided with a main emission lens and at least one auxiliary emission lens, each auxiliary emission lens corresponds to at least one LED light-emitting mechanism, namely, one of the LED light-emitting mechanisms emits light with a corresponding wave band, and the light is irradiated on a sample of the sample collection platform through the corresponding auxiliary emission lens, the main emission lens and the emission cylindrical lens in sequence.
Further, a dichroic mirror is used for each of the main emission lens and the sub emission lens so that each of the sub emission lenses emits light from the main emission lens.
Furthermore, four LED light-emitting mechanisms and two secondary emission lenses are arranged, namely, one secondary emission lens corresponds to each of the two LED light-emitting mechanisms.
Further, the LED light emitting mechanism includes: the light-emitting device comprises a light-emitting device, a first lens and a first optical filter; the light emitted by the light emitting device passes through the first lens and the first optical filter to emit light with corresponding wave bands.
Further, the PD receiving apparatus includes: the PD acquisition system comprises a PD receiving box, a plurality of PD photoelectric acquisition mechanisms, an incident lens group and an incident cylindrical lens; a receiving installation cavity is arranged in the PD receiving box, and each PD photoelectric acquisition mechanism, the incident lens group and the incident cylindrical lens are installed in the receiving installation cavity; the incident lens group is internally provided with a main incident lens and at least one secondary incident lens, each secondary incident lens corresponds to at least one PD photoelectric collection mechanism, and the detected light sequentially passes through the incident cylindrical lens, the main incident lens and each secondary incident lens and irradiates on each PD photoelectric collection mechanism so as to be detected.
Further, dichroic mirrors are used for the main entrance lens and the sub entrance lens so that the main entrance lens guides light into the sub entrance lenses, respectively.
Furthermore, four PD photoelectric acquisition mechanisms and two secondary incidence lenses are arranged, namely, one secondary incidence lens corresponds to each PD photoelectric acquisition mechanism.
Further, the PD photoelectric acquisition mechanism comprises: the optical sensor, the second lens and the second optical filter; the measured light sequentially passes through the second optical filter and the second lens to be irradiated on the photosensitive device so as to detect the measured light.
Furthermore, a sample placing groove is formed in the sample collecting platform, and the ejection cylindrical lens and the injection cylindrical lens are respectively located on two sides of the sample placing groove.
The beneficial effects of the utility model are that, the utility model discloses a set up the excitation light irradiation of the corresponding wave band of directional selection output of LED laser device on the sample of placing on the sample collection platform to the photometry that is sent through PD receiving arrangement receiving sample obtains the testing result, can satisfy the fluorescence detection demand of different wave bands, compact structure simultaneously, inseparable, simplify the light path design, reduce optical element's quantity and cost, optical system volume has been reduced, and use the scanning structure of fixed light path design substitution motion, make fluorescence repeatability higher.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic diagram of a micro multi-channel fluorescence detection system according to the present invention;
FIG. 2 is an internal structural view of the micro multi-channel fluorescence detection system of the present invention;
fig. 3 is a structural diagram of the LED excitation device of the present invention;
fig. 4 is a PD receiving apparatus according to the present invention.
In the figure:
1. an LED excitation device; 11. an LED excitation box; 12. an LED light emitting mechanism; 121. a light emitting device; 122. a first lens; 123. a first optical filter; 13. an ejection lens group; 131. a main emission lens; 132. a first secondary exit lens; 132. a second secondary exit lens; 14. emitting a cylindrical lens;
2. a PD receiving device; 21. a PD receiving cassette; 22. a PD photoelectric acquisition mechanism; 221. a photosensitive device; 222. a second lens; 223. a second optical filter; 23. an incident lens group; 231. a main incidence lens; 232. a first secondary entrance lens; 233. a second secondary entrance lens; 24. a cylindrical lens is shot into;
3. a sample collection platform; 31. and (4) a sample placing groove.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
In the present embodiment, as shown in fig. 1 to 4, the present embodiment provides a micro multi-channel fluorescence detection system, which includes: the device comprises an LED excitation device 1, a PD receiving device 2 and a sample collection platform 3; the LED excitation device 1 is provided with a plurality of LED light emitting mechanisms 12, and each LED light emitting mechanism 12 emits light of a corresponding wavelength band, and the light sequentially passes through the light emitting lens group 13 and the light emitting cylindrical lens 14 to irradiate on the sample of the sample collection platform 3, so that the sample is excited to emit a detected light, that is, the detected light sequentially passes through the light emitting cylindrical lens 24 and the light emitting lens group 23 to irradiate on each PD photoelectric collection mechanism 22 in the PD receiving device 2, so as to detect the detected light.
In this embodiment, this embodiment selects the excitation light of the corresponding wave band of output to shine on the sample of placing on sample collection platform 3 through setting up the orientation of LED laser device to receive the photometry that is sent by the sample and obtain the testing result through PD receiving arrangement 2, can satisfy the fluorescence detection demand of different wave bands, compact structure simultaneously, inseparable, simplify the light path design, reduce optical element's quantity and cost, optical system volume has been reduced, and use fixed light path design to replace the scanning structure of motion, make fluorescence repeatability higher.
In the present embodiment, the LED excitation device 1 includes: the LED illuminating device comprises an LED excitation box 11, a plurality of LED light-emitting mechanisms 12, an emergent lens group 13 and an emergent cylindrical lens 14; an excitation installation cavity is arranged in the LED excitation box 11, and each LED light-emitting mechanism 12, the emergent lens group 13 and the emergent cylindrical lens 14 are arranged in the excitation installation cavity; a main emitting lens 131 and at least one secondary emitting lens are arranged in the emitting lens group 13, and each secondary emitting lens corresponds to at least one LED light emitting mechanism 12, that is, one of the LED light emitting mechanisms 12 emits light of a corresponding wavelength band, and the light sequentially passes through the corresponding secondary emitting lens, the main emitting lens 131, and the emitting cylindrical lens 14 to irradiate on the sample of the sample collection platform 3.
In this embodiment, dichroic mirrors are used for the main emission lens 131 and the sub emission lenses so that the sub emission lenses emit light from the main emission lens 131.
In this embodiment, the primary exit lens 131 and the secondary exit lens each use a dichroic mirror to change the direction of light in each wavelength band.
In the present embodiment, four LED light emitting mechanisms 12 and two secondary emission lenses are provided, that is, one secondary emission lens corresponds to each of the two LED light emitting mechanisms 12.
In the present embodiment, the LED lighting mechanism 12 includes: a light emitting device 121, a first lens 122, and a first filter 123; the light emitted from the light emitting device 121 passes through the first lens 122 and the first filter 123 to emit light of a corresponding wavelength band.
In the present embodiment, the light emitting device 121 may be an LED, a halogen lamp, a laser, or the like.
In the present embodiment, the four LED lighting mechanisms 12 respectively emit light in four wavelength bands, and the light emitting devices 121 in the four LED lighting mechanisms 12 are respectively defined as LED1, LED2, LED3, and LED 4; when the collection is started, the LED1, the LED2, the LED3 and the LED4 are sequentially turned on, and when the LED1 is turned on, light is emitted from the corresponding LED light-emitting mechanism 12, passes through the first secondary emission lens 132, the main emission lens 131 and the emission cylindrical lens 14, and is irradiated on the sample collection platform 3; when the LED2 is turned on, light emitted from the corresponding LED lighting mechanism 12 passes through the first secondary exit lens 132, the main exit lens 131, and the exit cylindrical lens 14 to irradiate on the sample collection platform 3; when the LED3 is turned on, light emitted from the corresponding LED lighting mechanism 12 passes through the second secondary exit lens 132, the main exit lens 131, and the exit cylindrical lens 14 to irradiate on the sample collection platform 3; when the LED4 is lit, light is emitted from the corresponding LED lighting mechanism 12, passes through the second secondary exit lens 132, the main exit lens 131, and the exit cylindrical lens 14, and is irradiated on the sample collection stage 3.
In the present embodiment, the PD receiving apparatus 2 includes: the PD receiver box 21, a plurality of PD photoelectric acquisition mechanisms 22, an incidence lens group 23 and an incidence cylindrical lens 24; a receiving installation cavity is arranged in the PD receiving box 21, and each PD photoelectric acquisition mechanism 22, the incident lens group 23 and the incident cylindrical lens 24 are installed in the receiving installation cavity; the incident lens group 23 is provided with a main incident lens 231 and at least one secondary incident lens, and each secondary incident lens corresponds to at least one PD photoelectric pickup mechanism 22, that is, the detected light sequentially passes through the incident cylindrical lens 24, the main incident lens 231, and each secondary incident lens and is irradiated on each PD photoelectric pickup mechanism 22, so as to detect the detected light.
In this embodiment, dichroic mirrors are used for both the main entrance lens 231 and the sub entrance lens, so that the main entrance lens 231 guides light into each sub entrance lens.
In this embodiment, the primary incidence lens 231 and the secondary incidence lens each use a dichroic mirror to change the direction of light in each wavelength band.
In the present embodiment, four PD photoelectric detectors 22 and two slave entrance lenses are provided, that is, two PD photoelectric detectors 22 correspond to one slave entrance lens.
In this embodiment, the PD photoelectric pickup mechanism 22 includes: a photosensor 221, a second lens 222, and a second filter 223; the light to be measured is irradiated onto the photosensor 221 through the second filter 223 and the second lens 222 in this order to detect the light to be measured.
In this embodiment, the photosensor 221 may be a photosensor such as a PD photosensor, a CCD, or a photodiode.
In the present embodiment, the four PD photoelectric collection mechanisms 22 respectively receive light of corresponding wavelength bands, and the photosensors 221 of the four PD photoelectric collection mechanisms 22 are respectively defined as PD1, PD2, PD3, and PD 4; when collection is started, after the LED1 is turned on, light to be detected is emitted from the sample on the sample collection platform 3, and sequentially passes through the incident cylindrical lens 24, the main incident lens 231, the first secondary incident lens 232 and reaches the PD 1; when the LED2 is turned on, the detected light is emitted from the sample on the sample collection platform 3, and passes through the incident cylindrical lens 24, the main incident lens 231, the first secondary incident lens 232 and reaches the PD2 in sequence; when the LED3 is turned on, the light to be detected is emitted from the sample on the sample collection platform 3, and sequentially passes through the incident cylindrical lens 24, the main incident lens 231, and the second auxiliary incident lens 233 to reach the PD 3; when the LED4 is turned on, the light to be detected is emitted from the sample on the sample collection platform 3, and passes through the incident cylindrical lens 24, the main incident lens 231, and the second auxiliary incident lens 233 in this order to reach the PD 4.
In this embodiment, the sample collection platform 3 is provided with a sample placement groove 31, and the emergent cylindrical lens 14 and the incident cylindrical lens 24 are respectively located at two sides of the sample placement groove 31. To sum up, the utility model discloses a set up the directional excitation light that selects the corresponding wave band of output of LED laser device and shine on the sample of placing on the sample collection platform to receive the measured light that the sample sent through PD receiving arrangement and obtain the testing result, can satisfy the fluorescence detection demand of different wave bands, compact structure simultaneously, closely, simplify the light path design, reduce optical element's quantity and cost, reduced optical system volume, and use fixed light path design to replace the scanning structure of motion, make fluorescence repeatability higher; the optical structure is precise and small; the fluorescence of a plurality of channels can be collected simultaneously; the movement structure can be reduced, so that the fluorescence fluctuation is avoided; the loss in the optical path transmission process can be reduced to the greatest extent.
The components selected for use in the present application (components not illustrated for specific structures) are all common standard components or components known to those skilled in the art, and the structure and principle thereof can be known to those skilled in the art through technical manuals or through routine experimentation. Moreover, the software programs referred to in the present application are all prior art, and the present application does not relate to any improvement of the software programs.
In the description of the embodiments of the present invention, unless explicitly stated or limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the several embodiments provided in the present application, it should be understood that the disclosed system, apparatus and method may be implemented in other ways. The above-described embodiments of the apparatus are merely illustrative, and for example, the division of the units is only one logical division, and there may be other divisions when actually implemented, and for example, a plurality of units or components may be combined or integrated into another system, or some features may be omitted, or not executed. In addition, the shown or discussed coupling or direct coupling or communication connection between each other may be through some communication interfaces, indirect coupling or communication connection between devices or units, and may be in an electrical, mechanical or other form.
The units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of the embodiment.
In addition, functional units in the embodiments of the present invention may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit.
In light of the foregoing, it will be apparent to those skilled in the art from this disclosure that various changes and modifications can be made without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the description, and must be determined according to the scope of the claims.
Claims (10)
1. A miniature multi-channel fluorescence detection system, comprising:
the device comprises an LED excitation device, a PD receiving device and a sample collection platform; wherein
The LED excitation device is provided with a plurality of LED light-emitting mechanisms, and the corresponding LED light-emitting mechanisms emit light with corresponding wave bands, and the light irradiates on the sample of the sample collection platform through the ejection lens group and the ejection cylindrical lens in sequence so that the sample is excited to be measured, namely the light is excited to be measured
The detected light irradiates each PD photoelectric acquisition mechanism in the PD receiving device through the incident cylindrical lens and the incident lens group in sequence so as to detect the detected light.
2. The micro multi-channel fluorescence detection system of claim 1,
the LED excitation device comprises: the LED illuminating device comprises an LED excitation box, a plurality of LED light-emitting mechanisms, an emergent lens group and an emergent cylindrical lens;
an excitation installation cavity is formed in the LED excitation box, and the LED light-emitting mechanisms, the emergent lens group and the emergent cylindrical lens are installed in the excitation installation cavity;
a main emission lens and at least one secondary emission lens are arranged in the emission lens group, and each secondary emission lens corresponds to at least one LED light-emitting mechanism, namely
And one of the LED light-emitting mechanisms emits light with a corresponding wave band, and the light sequentially passes through the corresponding secondary emission lens, the main emission lens and the emission cylindrical lens to irradiate on a sample of the sample collection platform.
3. The miniature multi-channel fluorescence detection system of claim 2,
the main emission lens and the sub emission lens are dichroic mirrors so that the sub emission lenses emit light from the main emission lens.
4. The miniature multi-channel fluorescence detection system of claim 2,
with four said LED light-emitting means, two secondary exit lenses, i.e.
And the two LED light-emitting mechanisms correspond to one secondary emission lens.
5. The micro multi-channel fluorescence detection system of claim 2,
the LED light emitting mechanism includes: the light-emitting device comprises a light-emitting device, a first lens and a first optical filter;
the light emitted by the light emitting device passes through the first lens and the first optical filter to emit light with corresponding wave bands.
6. The micro multi-channel fluorescence detection system of claim 1,
the PD receiving apparatus includes: the PD acquisition system comprises a PD receiving box, a plurality of PD photoelectric acquisition mechanisms, an incident lens group and an incident cylindrical lens;
a receiving installation cavity is arranged in the PD receiving box, and each PD photoelectric acquisition mechanism, the incident lens group and the incident cylindrical lens are installed in the receiving installation cavity;
a main incidence lens and at least one secondary incidence lens are arranged in the incidence lens group, and each secondary incidence lens corresponds to at least one PD photoelectric acquisition mechanism, that is to say
The measured light sequentially passes through the incident cylindrical lens, the main incident lens and the auxiliary incident lenses and is irradiated on the PD photoelectric collecting mechanisms so as to be detected.
7. The micro multi-channel fluorescence detection system of claim 6,
the main incidence lens and the auxiliary incidence lens adopt dichroic mirrors so that the main incidence lens guides light to enter each auxiliary incidence lens.
8. The micro multi-channel fluorescence detection system of claim 6,
four PD photoelectric acquisition mechanisms and two secondary incidence lenses are arranged, namely
And the two PD photoelectric acquisition mechanisms correspond to one secondary incident lens.
9. The micro multi-channel fluorescence detection system of claim 6,
the PD photoelectric acquisition mechanism comprises: the optical sensor, the second lens and the second optical filter;
the measured light sequentially passes through the second optical filter and the second lens to be irradiated on the photosensitive device so as to detect the measured light.
10. The micro multi-channel fluorescence detection system of claim 6,
the sample collection platform is provided with a sample placing groove, and the ejection cylindrical lens and the injection cylindrical lens are respectively positioned on two sides of the sample placing groove.
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| CN202220237452.1U CN217237745U (en) | 2022-01-28 | 2022-01-28 | Miniature multichannel fluorescence detection system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115231202A (en) * | 2022-09-21 | 2022-10-25 | 儒克生物科技常州有限公司 | Conveying system for fluorescence detection and working method |
| CN115290567A (en) * | 2022-09-29 | 2022-11-04 | 儒克生物科技常州有限公司 | Sealed conveying system for fluorescence detection and working method thereof |
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2022
- 2022-01-28 CN CN202220237452.1U patent/CN217237745U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115231202A (en) * | 2022-09-21 | 2022-10-25 | 儒克生物科技常州有限公司 | Conveying system for fluorescence detection and working method |
| CN115290567A (en) * | 2022-09-29 | 2022-11-04 | 儒克生物科技常州有限公司 | Sealed conveying system for fluorescence detection and working method thereof |
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