CN102128830A - Immunochromatographic test paper strip color signal quantitative detector - Google Patents
Immunochromatographic test paper strip color signal quantitative detector Download PDFInfo
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- CN102128830A CN102128830A CN2010105911120A CN201010591112A CN102128830A CN 102128830 A CN102128830 A CN 102128830A CN 2010105911120 A CN2010105911120 A CN 2010105911120A CN 201010591112 A CN201010591112 A CN 201010591112A CN 102128830 A CN102128830 A CN 102128830A
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Abstract
The invention relates to an immunochromatographic test paper strip color signal quantitative detecting device, which comprises a lighting light source (1), a nontransparent shell (2), a transform lens (7), a slit (6), a photodetector (5), a primary amplification and alternating and direct (AD) control circuit (4) and a communication interface (3). By moving relative to a test paper strip along an axial direction of the test paper strip, the detector can quickly measure the detection line (T), quality control line (C) and light reflection strength of a back bottom part of the test paper strip. A connecting structure (8) is connected with different positioning accessories, so that the detector can adapt to detection of color signals of different package test paper strips. The detector has the characteristics of simple and compact structure, easy implementation, high sensitivity and low cost, and is convenient for multi-path integration or use in a handheld detection system.
Description
Technical field
This patent relates to the quantitative photodetector of biochemistry detection, relates in particular to a kind of immunochromatographydetecting detecting test strip colour developing weight detecting device that detects based on the location intensity of reflected light.
Background technology
Faulk in 1971 and Taytor introduce immunoassay with collaurum, formed immuno-gold labeling technology (Immunogoldlabeling techique), being about to collaurum and being applied to the antigen-antibody mark as tracer, mainly is that carrier is realized immune detection with the nitrocellulose filter.Colloidal gold immunochromatographimethod is analyzed (dot immunochro-matography assay, DICA) utilize the capillarity principle and make test solution chromatography diffusion and realized that sample matrix separates, so sample substrate disturbs little and is widely used in the fields such as quick diagnosis, health quarantine, the animal and plant quarantine, food safety detection, environmental monitoring of disease.Traditional colloidal gold immuno-chromatography test paper strip sensitivity and detection accuracy rate are on the low side, can only carry out qualitative detection.Test strips is equipped with microminiaturized detecting instrument and further improves detection sensitivity, realizes that multiple analyte detects, realizes quantitatively or half-quantitative detection is the developing direction of immunochromatographiassays assays.
In order to realize detection line color depth detection by quantitative to test strips, formerly technology " method and system and the application thereof of intelligent identification and reading immunity-chromatography " (CN101769925A), " a kind of portable extendible trial-production analytical approach and equipment " (CN101614672A) " test paper tape of colloidal gold in use for detecting residue number of degrees meter " (CN10109316A), adopt CCD " baffled photometer of gold-marking immunity test strips " (CN1904592A) " the employed detection method of a kind of immunity test strip bar colourity detection by quantitative instrument " (CN1566931A) " the reflective detector of test paper " (200920110847.X) wait and proposed some solutions.Can be divided into photoelectric diode or triode detector or the two kinds of schemes of employing ccd detector of adopting from detector angle.Adopting photodiode or triode is that cost is low as the detecting device advantage, and control circuit is simple, but can not distinguish surveyed area and background on every side to it, so detecting reliability is poor; Though and the employing ccd detector can extract tested scope effectively, its control is complicated, cost is high, has offset test strips advantage cheaply.In order effectively to improve photodiode or triode scheme detection sensitivity and reliability, the employing that has in the technology formerly the light-resource fousing scheme, but this scheme light path relative complex, must have higher requirements to light source in order to obtain small light spot, improved cost on the contrary, simultaneity factor is integrated, and aspects such as test strips contraposition have all increased difficulty.
Summary of the invention
The invention provides a kind of modular immunity test strip detection line color signal detection by quantitative detector, simple in structure, volume is little, and cost is low, utilize lens image position pupil filtering method can solve the paper slip background interference problem of inspecting effectively simultaneously, improve detection by quantitative sensitivity and reliability; Utilize A/D convertor circuit, detector module can directly provide the digitizing testing result.Can be used in multi-channel detection system or the hand system by integrated.
Technical solution of the present invention: the present invention is made up of light source (1), light tight shell (2), transform lens (7), slit (6), photo-detector (5), elementary amplifying circuit and AD control circuit (4) and communication interface (3).Light source (1) is used to the test strips of throwing light on, and can adopt LED or other luminaire; Light tight shell (2) provides support for whole module, and the isolating exterior parasitic light also prevents that the light source direct projection from surveying light path, and this light tight shell (2) also is used to provide and the plugging into of external system and test strips positioning fixture; Transform lens (7) is used to constitute the imaging optical path system, when the test strips detection line is positioned at beyond 1 times of focal length of transform lens (7) one sides certain plane, will become real image at its opposite side; Slit (6) is installed in test strips and becomes the real image position, the light path that transform lens (7) and slit (6) are combined into can realize filter effect, makes detector (5) thereafter can only receive the light signal that slit (6) is reflected in transform lens (7) opposite side object-image conjugate place (one narrow line zone); Elementary amplifying circuit and AD control circuit (4) are used for light signal conditioning amplification and are converted to digital signal; Communication interface (3) is used for control signal and exchanges data.
The present invention program's know-why is, by transform lens (7) to the test strips imaging, and in the picture plane filtering, realize the extraction in particular detection zone on the test strips, by test strips and detector relative displacement of the present invention, but continuous acquisition test strips detection line, background reflectance light intensity curve data, and after digitizing, export.
Compare the present invention with technology formerly and have following technique effect:
1, the invention provides and a kind ofly simply separate the spy solution with detection line based on the background of photodiode triode scheme.
2, the invention solves the requirement that light source in the technology formerly must optically focused and overlapped with detector window, simplified panel detector structure, be implemented in when improving detection sensitivity and further reduce cost, improve the purpose of reliability.
3, cost of the present invention is low, and compact conformation has digital interface, and adaptability is strong, is convenient to integrated application, has a good application prospect.
The present invention can be used for the detection components of food safety detection, health quarantine and animal and plant quarantine field hyperchannel or portable test strips detection system.The present invention also is applicable to the quantitative half-quantitative detection of other colour developing test strips color signals.
Description of drawings
By drawings and Examples, the technical scheme of this patent is described in further detail.Feature of the present invention will be by fully open or clear reflection, and wherein identical label refers to identical part, and wherein:
Fig. 1 is a structural drawing of the present invention.
Fig. 2 is the example structure figure of this patent.
Embodiment
The white light LEDs that light source (1) can adopt 1 piece of symmetry to place; Light tight shell (2) is a rectangle, and is provided with fixed orifice (9) and the draw-in groove (8) of the test strips positioning attachment that is used to plug into; Transform lens (7) adopts biconvex lens, and focal length is 10mm, diameter 10mm; Slit (6) width 1mm is positioned at (7) 2 times of focal planes of transform lens, and the test strips plane is positioned at 2 times of focal planes of transform lens (7) opposite side, and this scheme test strips imaging magnification is 1: 1, and object image distance is from the shortest; Detector (5) adopts photoelectric cell, is placed on slit (6) afterwards; The AVR ATMEGA32 single-chip microcomputer that elementary amplifying circuit and AD control circuit (4) adopt the single-stage pre-amplification circuit and have the AD function; Communication interface (3) adopts serial communication.This programme is realized simple, and compact conformation is convenient to integrated application.
Present embodiment only provides a kind of specific embodiments under method proposed by the invention instructs, do not limit this method embodiment and application, any do not break away from this method spirit to modification of the present invention and distortion, should be covered by within the institute of the present invention coverage.
Claims (5)
1. immunochromatographydetecting detecting test strip color signal detection by quantitative device, it is characterized in that, form by light source (1), light tight shell (2), transform lens (7), slit (6), photo-detector (5), elementary amplifying circuit and AD control circuit (4) and communication interface (3).
2. immunochromatographydetecting detecting test strip color signal detection by quantitative device, it is characterized in that being achieved as follows function: tested paper slip is placed with optical axis of the present invention is vertical, (7) 1 times of focal lengths of device transform lens are crossed with outerplanar in its plane, place, as the plane slit (6) is set in transform lens (7) opposite side correspondence, make the present invention and test strips occurred level to motion perpendicular to slit (6) direction, the present invention with recording light source (1) on test strips with slit (6) object-image conjugate regional reflex intensity variations; Handle by elementary amplifying circuit and AD control circuit (4), obtain quantizing detected value, export by communication interface (3).
3. according to the described light tight shell of claim 1, it is characterized in that: can isolation environment light influence to surveying; The light that light source (1) is sent must reflect the receiving light path of being allowed for access through test strips; Encapsulating whole detection architecture is convenient to integrated; Be provided with the replaceable different positioning attachment of syndeton (8) of conveniently plugging into.
4. according to the described slit of claim 1 (6), it is characterized in that: the opposite side test strips that is positioned at transform lens (7) is as the plane, and its slit direction is consistent with detection line direction on the test strips.
5. according to the described communication interface of claim 1, it is characterized in that: Interface for digital communication has data upwards transmission and the downward transfer function of control signal.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010591112 CN102128830B (en) | 2010-12-16 | 2010-12-16 | Immunochromatographic test paper strip color signal quantitative detector |
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| CN 201010591112 CN102128830B (en) | 2010-12-16 | 2010-12-16 | Immunochromatographic test paper strip color signal quantitative detector |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103163126A (en) * | 2011-12-08 | 2013-06-19 | 中国检验检疫科学研究院 | Quantitative detector for detecting test strip coloration signal through differential immunochromatography |
| CN103674859A (en) * | 2013-11-04 | 2014-03-26 | 上海感信信息科技有限公司 | Digital B-D (bowie-dick) test judgment system |
| CN104374767A (en) * | 2013-08-13 | 2015-02-25 | 嘉兴驰网工业自动化科技有限公司 | Portable type intelligent food safety detector |
| CN104977278A (en) * | 2014-04-04 | 2015-10-14 | 闽浪仪器科技（厦门）有限公司 | Quantitative analyzing and detecting system based on multispectral fluorescence immunochromatography |
| CN105044086A (en) * | 2015-04-23 | 2015-11-11 | 上海感信信息科技股份有限公司 | Pressure-steam sterilization chemical indication card color-change determination device |
| CN109298179A (en) * | 2018-11-29 | 2019-02-01 | 福州大学 | A kind of immunochromatography detection system and its Background Recognition method |
| CN111487229A (en) * | 2020-04-20 | 2020-08-04 | 中国农业科学院烟草研究所 | Plant germ detection device based on backlight excitation principle |
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| CN1754092A (en) * | 2003-02-26 | 2006-03-29 | 浜松光子学株式会社 | Device for measuring immunochromatography test piece |
| CN1851458A (en) * | 2005-04-22 | 2006-10-25 | 安捷伦科技有限公司 | Lateral flow assay systems and methods |
| CN1904592A (en) * | 2006-07-28 | 2007-01-31 | 中国科学院上海光学精密机械研究所 | Reflection photometer of gold label immune test paper |
| CN101769925A (en) * | 2009-12-22 | 2010-07-07 | 王继华 | Method and system for intelligently identifying and reading immunity-chromatography test strip and application thereof |
| JP2010197248A (en) * | 2009-02-25 | 2010-09-09 | Furukawa Electric Co Ltd:The | Apparatus for quantitatively detecting target substance used for lateral-flow type fluorescence immunochromatography |
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2010
- 2010-12-16 CN CN 201010591112 patent/CN102128830B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1754092A (en) * | 2003-02-26 | 2006-03-29 | 浜松光子学株式会社 | Device for measuring immunochromatography test piece |
| CN1851458A (en) * | 2005-04-22 | 2006-10-25 | 安捷伦科技有限公司 | Lateral flow assay systems and methods |
| CN1904592A (en) * | 2006-07-28 | 2007-01-31 | 中国科学院上海光学精密机械研究所 | Reflection photometer of gold label immune test paper |
| JP2010197248A (en) * | 2009-02-25 | 2010-09-09 | Furukawa Electric Co Ltd:The | Apparatus for quantitatively detecting target substance used for lateral-flow type fluorescence immunochromatography |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103163126A (en) * | 2011-12-08 | 2013-06-19 | 中国检验检疫科学研究院 | Quantitative detector for detecting test strip coloration signal through differential immunochromatography |
| CN104374767A (en) * | 2013-08-13 | 2015-02-25 | 嘉兴驰网工业自动化科技有限公司 | Portable type intelligent food safety detector |
| CN103674859A (en) * | 2013-11-04 | 2014-03-26 | 上海感信信息科技有限公司 | Digital B-D (bowie-dick) test judgment system |
| CN103674859B (en) * | 2013-11-04 | 2016-05-25 | 上海感信信息科技股份有限公司 | A kind of digital B-D test judgement system |
| CN104977278A (en) * | 2014-04-04 | 2015-10-14 | 闽浪仪器科技（厦门）有限公司 | Quantitative analyzing and detecting system based on multispectral fluorescence immunochromatography |
| CN105044086A (en) * | 2015-04-23 | 2015-11-11 | 上海感信信息科技股份有限公司 | Pressure-steam sterilization chemical indication card color-change determination device |
| CN105044086B (en) * | 2015-04-23 | 2018-08-28 | 上海感信信息科技股份有限公司 | A kind of discoloration of pressure steam sterilizing chemical tracer card judges equipment |
| CN109298179A (en) * | 2018-11-29 | 2019-02-01 | 福州大学 | A kind of immunochromatography detection system and its Background Recognition method |
| CN109298179B (en) * | 2018-11-29 | 2023-09-01 | 福州大学 | Immunochromatography detection system and background recognition method thereof |
| CN111487229A (en) * | 2020-04-20 | 2020-08-04 | 中国农业科学院烟草研究所 | Plant germ detection device based on backlight excitation principle |
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| CN102128830B (en) | 2013-03-13 |
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