CN102128830B - Immunochromatographic test paper strip color signal quantitative detector - Google Patents
Immunochromatographic test paper strip color signal quantitative detector Download PDFInfo
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- CN102128830B CN102128830B CN 201010591112 CN201010591112A CN102128830B CN 102128830 B CN102128830 B CN 102128830B CN 201010591112 CN201010591112 CN 201010591112 CN 201010591112 A CN201010591112 A CN 201010591112A CN 102128830 B CN102128830 B CN 102128830B
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Abstract
The invention relates to an immunochromatographic test paper strip color signal quantitative detecting device, which comprises a lighting light source (1), a nontransparent shell (2), a transform lens (7), a slit (6), a photodetector (5), a primary amplification and alternating and direct (AD) control circuit (4) and a communication interface (3). By moving relative to a test paper strip along an axial direction of the test paper strip, the detector can quickly measure the detection line (T), quality control line (C) and light reflection strength of a back bottom part of the test paper strip. A connecting structure (8) is connected with different positioning accessories, so that the detector can adapt to detection of color signals of different package test paper strips. The detector has the characteristics of simple and compact structure, easy implementation, high sensitivity and low cost, and is convenient for multi-path integration or use in a handheld detection system.
Description
Technical field
This patent relates to the quantitative photodetector of biochemistry detection, relates in particular to a kind of immunochromatographydetecting detecting test strip colour developing weight detecting device that detects based on the location intensity of reflected light.
Background technology
Faulk in 1971 and Taytor introduce immunoassay with collaurum, formed immuno-gold labeling technology (Immunogoldlabeling techique), be about to collaurum and be applied to the antigen-antibody mark as tracer, mainly realize immune detection take nitrocellulose filter as carrier.Highly-pathogenic avian influenza (dot immunochro-matography assay, DICA) utilize the capillarity principle and make test solution chromatography diffusion and realized that sample matrix separates, so sample substrate disturbs little and is widely used in the fields such as quick diagnosis, health quarantine, the animal and plant quarantine, food safety detection, environmental monitoring of disease.Traditional colloidal gold immuno-chromatography test paper strip sensitivity and Detection accuracy are on the low side, can only carry out qualitative detection.Test strips is equipped with microminiaturized detecting instrument and further improves detection sensitivity, realizes that multiple analyte detects, realizes quantitatively or half-quantitative detection is the developing direction of immunochromatographiassays assays.
In order to realize that the detection line color depth of test strips is quantitatively detected, formerly technology " method and system and the application thereof of intelligent identification and reading immunity-chromatography " (CN101769925A), " a kind of portable extendible trial-production analytical approach and equipment " (CN101614672A) " test paper tape of colloidal gold in use for detecting residue number of degrees meter " (CN10109316A), adopt CCD " baffled photometer of gold-marking immunity test strips " (CN1904592A) " the employed detection method of a kind of quantitative determination instrument for chroma of immunity detecting test strip " (CN1566931A) " test paper reflective detector " some solutions (200920110847.X) etc. have been proposed.Can be divided into employing photoelectric diode or triode detector or adopt the ccd detector two schemes from detector angle.Adopting photodiode or triode is that cost is low as the detecting device advantage, and control circuit is simple, but can not distinguish surveyed area and background on every side to it, so detecting reliability is poor; Although and the employing ccd detector can extract tested scope effectively, its control is complicated, cost is high, has offset cheaply advantage of test strips.For Effective Raise photodiode or triode scheme detection sensitivity and reliability, the employing that formerly has in the technology light-resource fousing scheme, but this scheme light path relative complex, must have higher requirements to light source in order to obtain small light spot, improved on the contrary cost, simultaneity factor is integrated, and the aspects such as test strips contraposition have all increased difficulty.
Summary of the invention
The invention provides a kind of modular immunity test strip detection line color signal and quantitatively detect detector, simple in structure, volume is little, and cost is low, utilize simultaneously lens image position pupil filtering method effectively to solve and inspect paper slip background interference problem, improve quantitative detection sensitivity and reliability; Utilize A/D convertor circuit, detector module can directly provide the digitizing testing result.Can be used in multi-channel detection system or the hand system by integrated.
Technical solution of the present invention: the present invention is comprised of light source (1), light tight shell (2), transform lens (7), slit (6), photo-detector (5), elementary amplifying circuit and AD control circuit (4) and communication interface (3).Light source (1) is used for the illumination test strips, can adopt LED or other luminaire; Light tight shell (2) provides support for whole module, and the isolating exterior parasitic light also prevents that the light source direct projection from surveying light path, and this light tight shell (2) also is used for providing and the plugging into of external system and test strips positioning fixture; Transform lens (7) be used for to consist of the imaging optical path system, during certain plane, will become real image at its opposite side beyond the ELISA test strip line is positioned at 1 times of focal length of transform lens (7) one sides; Slit (6) is installed in test strips and becomes the real image position, the light path that transform lens (7) and slit (6) are combined into can realize filter effect, makes detector (5) thereafter can only receive slit (6) light signal that (a bar narrow line is regional) reflects at transform lens (7) opposite side object-image conjugate place; Elementary amplifying circuit and AD control circuit (4) are used for light signal conditioning amplification and are converted to digital signal; Communication interface (3) is used for control signal and exchanges data.
The present invention program's know-why is, by transform lens (7) to the test strips imaging, and in the picture plane filtering, realize the extraction in particular detection zone on the test strips, by test strips and detector relative displacement of the present invention, but continuous acquisition ELISA test strip line, background reflectance light intensity curve data, and after digitizing, export.
Compare the present invention with technology formerly and have following technique effect:
1, the invention provides and a kind ofly simply separate the spy solution with detection line based on the background of photodiode triode scheme.
2, the invention solves the requirement that light source in the technology formerly must optically focused and overlapped with detector window, simplified panel detector structure, be implemented in and further reduce cost when improving detection sensitivity, improve the purpose of reliability.
3, cost of the present invention is low, and compact conformation has digital interface, and strong adaptability is convenient to integrated application, has a good application prospect.
The present invention can be used for the detection components of food safety detection, health quarantine and animal and plant quarantine field hyperchannel or portable ELISA test strip system.The present invention also is applicable to the quantitative half-quantitative detection of other colour developing test strips color signals.
Description of drawings
By drawings and Examples, the technical scheme of this patent is described in further detail.Feature of the present invention will be by fully open or clear reflection, and wherein identical label refers to identical part, and wherein:
Fig. 1 is structural drawing of the present invention.
Fig. 2 is the example structure figure of this patent.
Embodiment
Light source (1) can adopt 1 piece of symmetrical white light LEDs of placing; Light tight shell (2) is rectangle, and is provided with fixed orifice (9) and the draw-in groove (8) of the test strips positioning attachment that is used for plugging into; Transform lens (7) adopts biconvex lens, and focal length is 10mm, diameter 10mm; Slit (6) width 1mm is positioned at (7) 2 times of focal planes of transform lens, and the test strips plane is positioned at 2 times of focal planes of transform lens (7) opposite side, and this scheme test strips imaging magnification is 1: 1, and object image distance is from the shortest; Detector (5) adopts photoelectric cell, is placed on slit (6) afterwards; The AVR ATMEGA32 single-chip microcomputer that elementary amplifying circuit and AD control circuit (4) adopt the single-stage pre-amplification circuit and have the AD function; Communication interface (3) adopts serial communication.This programme is realized simple, and compact conformation is convenient to integrated application.
Present embodiment only provides a kind of specific embodiments under method proposed by the invention instructs, do not limit this method embodiment and application, any do not break away from this method spirit to modification of the present invention and distortion, should be covered by within the institute of the present invention coverage.
Claims (4)
1. immunochromatographitest test paper strip color signal quantitative detector is comprised of light source (1), light tight shell (2), transform lens (7), slit (6), photo-detector (5), elementary amplifying circuit and AD control circuit (4) and communication interface (3); It is characterized in that: tested paper slip is placed with the quantitative detector optical axis is vertical, its plane, place is positioned at the twice focal plane of transform lens (7) opposite side, the test strips imaging magnification is 1: 1, at picture plane layout slit (6) corresponding to transform lens (7) opposite side, make quantitative detector and the relative motion of test strips occurred level perpendicular to slit (6) direction, quantitative detector with recording light source (1) on test strips with the variation of slit (6) object-image conjugate regional reflex light intensity; Process by elementary amplifying circuit and AD control circuit (4), obtain quantizing detected value, export by communication interface (3).
2. described quantitative detector according to claim 1 is characterized in that: the impact of described light tight shell isolation environment light on surveying makes the light that light source (1) sends must be through the test strips reflection receiving light path of being allowed for access; Described light tight shell encapsulates whole detection architecture and is convenient to integrated; Described light tight shell is provided with the replaceable different positioning attachment of syndeton (8) of conveniently plugging into.
3. described quantitative detector according to claim 1, it is characterized in that: slit (6) is positioned at the opposite side test strips of transform lens (7) as the plane, and its slit direction is consistent with detection line direction on the test strips.
4. described quantitative detector according to claim 1, it is characterized in that: described communication interface (3) has data upwards transmission and the downward transfer function of control signal.
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| CN 201010591112 CN102128830B (en) | 2010-12-16 | 2010-12-16 | Immunochromatographic test paper strip color signal quantitative detector |
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| CN 201010591112 CN102128830B (en) | 2010-12-16 | 2010-12-16 | Immunochromatographic test paper strip color signal quantitative detector |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103163126A (en) * | 2011-12-08 | 2013-06-19 | 中国检验检疫科学研究院 | Quantitative detector for detecting test strip coloration signal through differential immunochromatography |
| CN104374767A (en) * | 2013-08-13 | 2015-02-25 | 嘉兴驰网工业自动化科技有限公司 | Portable type intelligent food safety detector |
| CN103674859B (en) * | 2013-11-04 | 2016-05-25 | 上海感信信息科技股份有限公司 | A kind of digital B-D test judgement system |
| CN104977278A (en) * | 2014-04-04 | 2015-10-14 | 闽浪仪器科技（厦门）有限公司 | Quantitative analyzing and detecting system based on multispectral fluorescence immunochromatography |
| CN105044086B (en) * | 2015-04-23 | 2018-08-28 | 上海感信信息科技股份有限公司 | A kind of discoloration of pressure steam sterilizing chemical tracer card judges equipment |
| CN109298179B (en) * | 2018-11-29 | 2023-09-01 | 福州大学 | Immunochromatography detection system and background recognition method thereof |
| CN111487229B (en) * | 2020-04-20 | 2023-05-23 | 中国农业科学院烟草研究所 | Plant pathogen detection device based on backlight excitation principle |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1851458A (en) * | 2005-04-22 | 2006-10-25 | 安捷伦科技有限公司 | Lateral flow assay systems and methods |
| CN1904592A (en) * | 2006-07-28 | 2007-01-31 | 中国科学院上海光学精密机械研究所 | Reflection photometer of gold label immune test paper |
| CN101769925A (en) * | 2009-12-22 | 2010-07-07 | 王继华 | Method and system for intelligently identifying and reading immunity-chromatography test strip and application thereof |
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| US7173705B2 (en) * | 2003-02-26 | 2007-02-06 | Hamamatsu Photonics K.K. | Measuring device for immunochromatography test piece |
| JP4676539B2 (en) * | 2009-02-25 | 2011-04-27 | 古河電気工業株式会社 | Apparatus and method for quantitatively detecting a target substance for lateral flow type fluorescent immunochromatography |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1851458A (en) * | 2005-04-22 | 2006-10-25 | 安捷伦科技有限公司 | Lateral flow assay systems and methods |
| CN1904592A (en) * | 2006-07-28 | 2007-01-31 | 中国科学院上海光学精密机械研究所 | Reflection photometer of gold label immune test paper |
| CN101769925A (en) * | 2009-12-22 | 2010-07-07 | 王继华 | Method and system for intelligently identifying and reading immunity-chromatography test strip and application thereof |
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| JP特开2010-197248A 2010.09.09 |
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