CN105866075A - Fluorescence detection apparatus - Google Patents

Fluorescence detection apparatus Download PDF

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Publication number
CN105866075A
CN105866075A CN201510024647.2A CN201510024647A CN105866075A CN 105866075 A CN105866075 A CN 105866075A CN 201510024647 A CN201510024647 A CN 201510024647A CN 105866075 A CN105866075 A CN 105866075A
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CN
China
Prior art keywords
light
light source
unit
fluorescence
port
Prior art date
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Pending
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CN201510024647.2A
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Chinese (zh)
Inventor
邱宪波
乔杉
葛胜祥
阳勇良
牛亚男
闵小平
张晓磊
夏宁邵
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Beijing University of Chemical Technology
Xiamen University
Beijing WanTai Biological Pharmacy Enterprise Co Ltd
Original Assignee
Beijing University of Chemical Technology
Xiamen University
Beijing WanTai Biological Pharmacy Enterprise Co Ltd
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Application filed by Beijing University of Chemical Technology, Xiamen University, Beijing WanTai Biological Pharmacy Enterprise Co Ltd filed Critical Beijing University of Chemical Technology
Priority to CN201510024647.2A priority Critical patent/CN105866075A/en
Publication of CN105866075A publication Critical patent/CN105866075A/en
Pending legal-status Critical Current

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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The invention discloses a fluorescence detection apparatus. The apparatus mainly comprises a light source, an optical fiber, a photoelectric conversion unit, and a concentration detection unit, wherein the light source is used for generating detection light, in order to carry out irradiation for test paper; the optical fiber is used for transmitting fluorescence generated from a fluorescent maker on the test paper after excitation due to irradiation by the detection light to the photoelectric conversion unit; the photoelectric conversion unit is used for converting received fluorescence into an electric signal and sending the electric signal to the concentration detection unit; and the concentration detection unit is used for determining concentration of a target object on the test paper according to the received electric signal. The apparatus has advantages of effectively simplified structure of the fluorescence detection apparatus, reduced cost, reduced complexity of assembling and debugging, and improved reliability of the apparatus.

Description

Fluorescence detection device
Technical field
The present invention relates to detection field, particularly relate to a kind of fluorescence detection device.
Background technology
The principle of immune analysis method is based on specific binding between antigen-antibody, to testing sample Target substance in (whole blood, serum, saliva etc.) qualitatively or quantitatively detects.At present, Immunologic detection method on Clinical Laboratory market mostly exist sensitivity low, can not quantitatively or quantitatively Inaccurate problem.Immune detection based on fluorescent labeling principle has highly sensitive, quantification range Wide advantage, is just occupying the status become more and more important in Clinical Laboratory market.
In prior art, the excitation light path of fluorescence detection device uses mostly with fluorescence detection optical path The mode that battery of lens focuses on, for different light paths (excite, launch), generally requires and makes With different battery of lens.In order to obtain preferable optical detection performance, several in battery of lens Lens, such as planoconvex lens, dichroscope, cylindrical mirror etc. need according to principle design, according to lens it Between position, the spatial relationship such as angle, accurately lens are fixed on high-precision optical combination In module.Therefore, this kind of apparatus structure is complicated, cost is high, it is high to assemble and debug complexity, It is unfavorable for realization and the popularization of fluorescence detection method.
Summary of the invention
The invention provides a kind of fluorescence detection device, it is possible to effectively simplify the knot of fluorescence detection device Structure, reduces cost, reduces assembling and debugging complexity, improves device reliability.
According to the present invention, it is provided that a kind of fluorescence detection device, turn including light source, optical fiber, photoelectricity Change unit and concentration detecting unit, wherein:
Light source, is used for producing detection light, in order to be irradiated reagent paper;
Optical fiber, excites the fluorescence of generation after the fluorescent marker detected light on reagent paper being irradiated It is sent to photoelectric conversion unit;
Photoelectric conversion unit, for the fluorescence received is converted to the signal of telecommunication, and sends out the signal of telecommunication Give concentration detecting unit;
Concentration detecting unit, for determining the dense of object on reagent paper according to the signal of telecommunication that receives Degree.
In one embodiment, exciting light shaping unit is also included, wherein:
Exciting light shaping unit, carries out shaping for the detection light producing light source.
In one embodiment, exciting light shaping unit has logical optical port, the detection that light source produces Light is irradiated on reagent paper by logical optical port.
In one embodiment, lead to optical port is shaped as strip, in order to the detection light that light source produces Linear light is formed at logical optical port.
In one embodiment, the first optical filter is set between light source and exciting light shaping unit, Detection light for producing light source filters.
In one embodiment, optical fiber has the first port and the second port, and wherein fluorescence is by the Single port enters optical fiber, and arrives photoelectric conversion unit by the second port.
In one embodiment, between the second port and the photoelectric conversion unit of optical fiber, second is arranged Optical filter, for filtering the fluorescence entering photoelectric conversion unit.
In one embodiment, any of the above-described device related to, also include light source monitoring unit and bright Degree regulation unit, wherein:
Light source monitoring unit, the reflection light produced on reagent paper pallet for acquisition testing light, will adopt Collect to reflection light be converted to the signal of telecommunication, and the signal of telecommunication is sent to brightness adjusting unit;
The brightness of light source is carried out by the signal of telecommunication that brightness adjusting unit sends according to light source monitoring unit Regulation.
In one embodiment, also include that linear electric motors, guide rail and the scanning being arranged on guide rail are put down Platform, wherein:
Light source, exciting light shaping unit, optical fiber, photoelectric conversion unit and light source monitoring unit are arranged On scanning platform;
Linear electric motors operationally, drive scanning platform to move on guide rail, in order to realize reagent paper Scanning Detction.
In one embodiment, the concentration detecting unit that any of the above-described embodiment relates to has with extraneous The communication interface interacted.
The fluorescence detection device of the present invention, it is possible to effectively simplify the structure of fluorescence detection device, reduces Cost, reduces assembling and debugging complexity, improves device reliability.
Accompanying drawing explanation
For the technical scheme being illustrated more clearly that in the embodiment of the present invention, below will to embodiment or In description, the required accompanying drawing used is briefly described, it should be apparent that, attached in describing below Figure is only some embodiments of the present invention, for those of ordinary skill in the art, is not paying On the premise of going out creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is one embodiment schematic diagram of fluorescence detection device of the present invention.
Fig. 2 is light source and the profile of exciting light Shaping Module in fluorescence detection device of the present invention.
Fig. 3 is the schematic diagram of fluorescence detection device light source process of self-test of the present invention.
Fig. 4 is the schematic diagram of another embodiment of fluorescence detection device of the present invention.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, to the technical scheme in the embodiment of the present invention It is clearly and completely described, it is clear that described embodiment is only that a part of the present invention is real Execute example rather than whole embodiments.Description at least one exemplary embodiment is real below It is merely illustrative on border, never as to the present invention and application thereof or any limit of use System.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creative labor The every other embodiment obtained under dynamic premise, broadly falls into the scope of protection of the invention.
Unless specifically stated otherwise, the parts illustrated the most in these embodiments and the phase of step Layout, numerical expression and numerical value are not limited the scope of the invention.
Simultaneously, it should be appreciated that for the ease of describing, the chi of the various piece shown in accompanying drawing Very little is not to draw according to actual proportionate relationship.
May not make in detail for technology, method and apparatus known to person of ordinary skill in the relevant Thin discussion, but in the appropriate case, described technology, method and apparatus should be considered to authorize to be said A part for bright book.
Shown here with in all examples discussed, any occurrence should be construed as merely Exemplary rather than conduct limits.Therefore, other example of exemplary embodiment can have There is different values.
It should also be noted that similar label and letter expression similar terms in following accompanying drawing, therefore, The most a certain Xiang Yi accompanying drawing is defined, then need not it is carried out in accompanying drawing subsequently Discussed further.
Fig. 1 is one embodiment schematic diagram of fluorescence detection device of the present invention.As it is shown in figure 1, it is glimmering Optical detection device 100 includes light source 1, optical fiber 3, photoelectric conversion unit 4 and Concentration Testing list Unit 7.
Light source 1, is used for producing detection light, in order to be irradiated reagent paper 9.
Optical fiber 3, excites generation after the fluorescent marker detected light on reagent paper 9 being irradiated Fluorescence is sent to photoelectric conversion unit 4.
Further, optical fiber 3 includes the first port 31 and the second port 32.Fluorescent marker quilt Detection light excites the fluorescence of generation to enter optical fiber 3 by the first port 31 after irradiating, and by the Two-port netwerk 32 arrives photoelectric conversion unit 4.
Photoelectric conversion unit 4, for being converted to the signal of telecommunication by the fluorescence received, and by the signal of telecommunication It is sent to concentration detecting unit 7.
Concentration detecting unit 7, for determining object on reagent paper 9 according to the signal of telecommunication received Concentration.
The fluorescence detection device provided based on the above embodiment of the present invention, by produce light source 1 Detection light is radiated on the fluorescent marker in reagent paper 9 generation fluorescence, and by fluorescence by optical fiber 3 It is sent in photoelectric conversion unit 4, is converted to the signal of telecommunication through photoelectric conversion unit 4 and is sent to concentration Detector unit 7, concentration detecting unit 7 determines the concentration of object according to the signal of telecommunication received.By The detection light sent in light source 1 is had no backing lens, cylindrical mirror or fiber-optic illuminated on reagent paper 9, The fluorescence that fluorescent marker on reagent paper 9 produces simultaneously is collected and passed to opto-electronic conversion by optical fiber 3 Unit 4, does not utilize condenser lens, therefore can effectively simplify the structure of fluorescence detection device, User be no longer necessary to according to each lens between position, the spatial relationship such as angle, accurately will be thoroughly Mirror is fixed in high-precision optical combination module, thus reduces answering of device debugging and assembling Miscellaneous degree, reduces cost, improves the reliability of device.
Preferably, light source 1 is LED (Light Emitting Diode, Light-Emitting Diode).? In one preferred embodiment, the fluorescent marker excitation center wavelength of selection is 365nm, light source 1 The detection optical wavelength range provided is 360-410nm.This range of wavelengths is as detection light source, energy Higher, when the fluorescence detection device detection using the present invention, it is no longer necessary to come by lens arrangement Improve detection sensitivity.
Preferably, the fluorescent marker on reagent paper 9 is irradiated by light source 1 with 45 degree of angles.
Preferably, the wavelength of fluorescence that fluorescent marker produces is 615nm.
Owing to the present invention directly collects fluorescence by optical fiber 3, therefore, with the side using lens combination Formula is compared, and the advantage of the present invention, can be by optical fiber 3 and examination in addition to reducing complexity and cost It is the least, to guarantee that optical fiber 3 is in the case of without condenser lens, it is ensured that fluorescence that the distance of paper 9 is adjusted Collection efficiency.Preferably, the first port 31 of optical fiber 3 to fluorescent marker on reagent paper 9 it Between distance be 2.5-3.0mm.
Preferably, the first port 31 of optical fiber 3 and the cross sectional shape of the second port 32 can bases Needs are adjusted.
In one embodiment, the fluorescence detection device of the present invention also includes exciting light Shaping Module 2, carry out shaping for the detection light that light source 1 is produced.Fig. 2 is the fluoroscopic examination dress of the present invention Put the profile of middle light source 1 and exciting light Shaping Module 2.As in figure 2 it is shown, exciting light shaping Module 2 includes logical optical port 21, adjusts for light source 1 generation detects the shape of light Whole, the detection light that light source 1 produces is irradiated on reagent paper 9 by logical optical port 21.
In one embodiment, the outlet of logical optical port 21 can be point-like, strip or other Shape, in order to the detection light that light source 1 produces forms point source, line source at logical optical port 21 Or the detection light source of other shapes.
Preferably, lead to optical port 21 is shaped as strip, in order to the detection light that light source 1 produces exists Linear light is formed at logical optical port 21.When detecting light and being linear, there is specific advantage.Right For point source, owing to optical fiber being converged on a point or a circle, and fluorescent marker Usually strip, therefore carries out spot scan with point source and is only capable of getting the fluorescence information of part.And For line source, the fluorescent marker of strip can be carried out complete by produced linear light Scanning, when therefore having the fluorescent marker of multiple strip on reagent paper 9, it is possible to single pass arrives, Thus improve scan efficiency.
Preferably, logical distance between optical port 21 and light source 1 is 2.5-3.5mm.
Preferably, logical optical port 21 width is 0.1-0.5mm, a length of 1.5-2.5mm.
In one embodiment, the fluorescence detection device of the present invention also includes light source monitoring unit 5 With brightness adjusting unit 12.Fig. 3 is the signal of fluorescence detection device light source process of self-test of the present invention Figure.Fig. 4 is the schematic diagram of another embodiment of fluorescence detection device of the present invention.In conjunction with Fig. 3 and Tu 4, in the present embodiment, the detection light that light source 1 sends is irradiated on reagent paper pallet 8, through reagent paper After pallet 8 reflection, part detection light enters into light source monitoring unit 5, and light source monitoring unit 5 will The reflection light collected is converted to the signal of telecommunication, and the signal of telecommunication is sent to brightness adjusting unit 12, The brightness of light source 1 is entered by the signal of telecommunication that brightness adjusting unit 12 sends according to light source monitoring unit 5 Row regulation, does not affect the normal work of fluorescence detection device.Light source monitoring unit 5 and brightness regulation Unit 12 when fluorescence detection device works, can monitor the duty of light source 1 in real time, and Time the regulation detection brightness that sends of light source 1.Those skilled in the art are it will be appreciated that brightness Regulation unit 12 is not limited only to be adjusted the brightness of light source 1, it would however also be possible to employ alerts and waits it The duty of light source 1 is notified or regulates form by he.
Preferably, as shown in Figure 4, between light source 1 and exciting light shaping unit 2, first is arranged Optical filter 10, filters for the detection light producing light source.Preferably, the first optical filter The wave-length coverage of the light that 10 permissions are passed through is 310-410nm.
Preferably, as shown in Figure 4, at the second port 32 and the photoelectric conversion unit 4 of optical fiber 3 Between the second optical filter 11 is set, for enter photoelectric conversion unit 4 fluorescence filter. Preferably, the second optical filter 11 allows the wave-length coverage of the light passed through to be more than 580nm, mainly For avoiding the detection light of light source 1 to enter photoelectric conversion unit 4.
Preferably, the fluorescence detection device of the present invention also includes that linear electric motors, guide rail and being arranged on is led Scanning platform on rail, wherein:
Light source 1, exciting light shaping unit 2, optical fiber 3, photoelectric conversion unit 4 and light source monitoring Unit 5 is arranged on scanning platform.
Linear electric motors operationally, drive scanning platform to move on guide rail, in order to realize reagent paper The Scanning Detction of 9.
The present invention is by by light source 1, exciting light shaping unit 2, optical fiber 3, photoelectric conversion unit 4 and light source monitoring unit 5 be arranged on scanning platform, linear electric motors drive scanning platform at guide rail Upper movement, in order to realize the Scanning Detction to reagent paper 9, further simplify the frame for movement of device, Reduce the volume of device.
Preferably, the concentration detecting unit 7 that any of the above-described embodiment relates to has to be handed over the external world Mutual communication interface, such as, for the printer interface of external printer, measure for real time print Data;Network interface, for real-time Communication for Power;Display interface device etc. for external-connection displayer.
As a example by Fig. 4, the present invention will be described below.
Light source 1 sends detection light, enters exciting light Shaping Module after the first optical filter 10 filters 2, in order to only allow the detection light meeting respective wavelength area requirement from exciting light Shaping Module 2 Logical optical port 21 penetrates.The logical optical port 21 of exciting light Shaping Module 2 is strip, will detect light It is shaped as linear light to be radiated on the fluorescent marker of reagent paper 9.Light source 1, exciting light shaping unit 2, optical fiber 3, photoelectric conversion unit 4 and light source monitoring unit 5 are arranged on scanning platform, directly Line motor operationally, drives scanning platform to move on guide rail, in order to realize sweeping reagent paper 9 Retouch detection.
Reagent paper 9 is placed on reagent paper pallet 8, and the fluorescent marker detected light in reagent paper 9 is irradiated The fluorescence of rear generation enters optical fiber 3 by the first port 31 of optical fiber 3, and by the second port 32 arrive photoelectric conversion unit 4.
The fluorescence received is converted to the signal of telecommunication by photoelectric conversion unit 4, and is sent to by the signal of telecommunication Concentration detecting unit 7, concentration detecting unit 7 is for determining reagent paper according to the signal of telecommunication received The concentration of upper object.
During fluorescence detection device works, the detection light that light source 1 sends is irradiated to reagent paper On pallet 8, after reagent paper pallet 8 reflects, part detection light enters into light source monitoring unit 5, The reflection light collected is converted to the signal of telecommunication by light source monitoring unit 5, and is sent to by the signal of telecommunication Brightness adjusting unit 12, the telecommunications that brightness adjusting unit 12 sends according to light source monitoring unit 5 Number the brightness of light source 1 is adjusted.
A kind of fluorescence detection device of the present invention, overcomes fluorescence detection device of the prior art The structure needing battery of lens incompatible convergence detection light and fluorescence to be brought is complicated, inconvenient being stranded of regulation Difficult, it is possible to effectively to simplify the structure of fluorescence detection device, reduce cost, reduce assembling and debugging Complexity, improves device reliability.
One of ordinary skill in the art will appreciate that all or part of step realizing above-described embodiment Suddenly can be completed by hardware, it is also possible to instruct relevant hardware by program and complete, institute The program stated can be stored in a kind of computer-readable recording medium, and storage mentioned above is situated between Matter can be read only memory, disk or CD etc..
Description of the invention is given for the sake of example and description, and is not exhaustively Or it limit the invention to disclosed form.Many modifications and variations are for the common skill of this area It is obvious for art personnel.Selecting and describing embodiment is the principle in order to the present invention is more preferably described Apply with reality, and make those of ordinary skill in the art it will be appreciated that the present invention thus design is suitable The various embodiments with various amendments in special-purpose.

Claims (10)

1. a fluorescence detection device, it is characterised in that include light source (1), optical fiber (3), Photoelectric conversion unit (4) and concentration detecting unit (7), wherein:
Light source (1), is used for producing detection light, in order to be irradiated reagent paper (9);
Optical fiber (3), swashs after the fluorescent marker detected light on reagent paper (9) being irradiated Send out the fluorescence produced and be sent to photoelectric conversion unit (4);
Photoelectric conversion unit (4), for the fluorescence received being converted to the signal of telecommunication, and will The signal of telecommunication is sent to concentration detecting unit (7);
Concentration detecting unit (7), for determining reagent paper (9) according to the signal of telecommunication received The concentration of object.
Device the most according to claim 1, it is characterised in that also include exciting finishing Shape unit (2), wherein:
Exciting light shaping unit (2), carries out shaping for the detection light producing light source (1).
Device the most according to claim 2, it is characterised in that
Exciting light shaping unit (2) has logical optical port (21), the inspection that light source (1) produces Light-metering is irradiated on reagent paper (9) by logical optical port (21).
Device the most according to claim 3, it is characterised in that
Lead to optical port (21) is shaped as strip, in order to the detection light that light source (1) produces exists Logical optical port (21) place forms linear light.
Device the most according to claim 4, it is characterised in that
First optical filter (10) is set between light source (1) and exciting light shaping unit (2), Detection light for producing light source (1) filters.
Device the most according to claim 1, it is characterised in that
Optical fiber (3) has the first port (31) and the second port (32), and wherein fluorescence leads to Cross the first port (31) and enter optical fiber (3), and arrive photoelectricity by the second port (32) Converting unit (4).
Device the most according to claim 6, it is characterised in that
Is arranged between second port (32) and photoelectric conversion unit (4) of optical fiber (3) Two optical filters (11), for filtering the fluorescence entering photoelectric conversion unit (4).
8. according to the device according to any one of claim 1-7, it is characterised in that also wrap Include light source monitoring unit (5) and brightness adjusting unit (12), wherein:
Light source monitoring unit (5), for acquisition testing light in the upper generation of reagent paper pallet (8) Reflection light, is converted to the reflection light collected the signal of telecommunication, and the signal of telecommunication is sent to brightness tune Joint unit (12);
The signal of telecommunication that brightness adjusting unit (12) sends according to light source monitoring unit (5) is to light The brightness in source (1) is adjusted.
Device the most according to claim 8, it is characterised in that also include linear electric motors, Guide rail and the scanning platform being arranged on guide rail, wherein:
Light source (1), exciting light shaping unit (2), optical fiber (3), photoelectric conversion unit (4) it is arranged on scanning platform with light source monitoring unit (5);
Linear electric motors operationally, drive scanning platform to move on guide rail, in order to realize examination The Scanning Detction of paper (9).
10. according to the device according to any one of claim 1-7, it is characterised in that
Concentration detecting unit (7) has and the extraneous communication interface interacted.
CN201510024647.2A 2015-01-19 2015-01-19 Fluorescence detection apparatus Pending CN105866075A (en)

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Application Number Priority Date Filing Date Title
CN201510024647.2A CN105866075A (en) 2015-01-19 2015-01-19 Fluorescence detection apparatus

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106645708A (en) * 2016-09-20 2017-05-10 必欧瀚生物技术(合肥)有限公司 Quantitative detection calculation method based on fluorescent immuno-chromatographic technology
CN106906137A (en) * 2017-03-19 2017-06-30 北京化工大学 A kind of high-throughput nucleic acid analysis device based on micro-fluidic chip
CN107543793A (en) * 2017-07-11 2018-01-05 国家电网公司 A kind of transformer oil storage capsule damage detection device
CN107919215A (en) * 2017-11-08 2018-04-17 国家电网公司 A kind of oil-immersed transformer

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106645708A (en) * 2016-09-20 2017-05-10 必欧瀚生物技术(合肥)有限公司 Quantitative detection calculation method based on fluorescent immuno-chromatographic technology
CN106906137A (en) * 2017-03-19 2017-06-30 北京化工大学 A kind of high-throughput nucleic acid analysis device based on micro-fluidic chip
CN106906137B (en) * 2017-03-19 2019-02-12 北京化工大学 A kind of high-throughput nucleic acid analysis device based on micro-fluidic chip
CN107543793A (en) * 2017-07-11 2018-01-05 国家电网公司 A kind of transformer oil storage capsule damage detection device
CN107919215A (en) * 2017-11-08 2018-04-17 国家电网公司 A kind of oil-immersed transformer

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Application publication date: 20160817