CN110174509A - A kind of spectral analysis device - Google Patents
A kind of spectral analysis device Download PDFInfo
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- CN110174509A CN110174509A CN201810248659.7A CN201810248659A CN110174509A CN 110174509 A CN110174509 A CN 110174509A CN 201810248659 A CN201810248659 A CN 201810248659A CN 110174509 A CN110174509 A CN 110174509A
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- 230000005540 biological transmission Effects 0.000 claims abstract description 29
- 241000251468 Actinopterygii Species 0.000 claims abstract description 9
- 238000009434 installation Methods 0.000 claims abstract description 5
- 238000005259 measurement Methods 0.000 claims description 21
- 238000010168 coupling process Methods 0.000 claims description 20
- 238000005859 coupling reaction Methods 0.000 claims description 20
- 230000003287 optical effect Effects 0.000 claims description 20
- 230000003595 spectral effect Effects 0.000 claims description 20
- 230000008878 coupling Effects 0.000 claims description 19
- 239000000523 sample Substances 0.000 claims description 19
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 239000004973 liquid crystal related substance Substances 0.000 claims description 7
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- 238000003018 immunoassay Methods 0.000 abstract description 3
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- Biotechnology (AREA)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention provides a kind of spectral analysis devices, it is related to fluorescence excitation and detection technique field, the analyzer includes the fish tail handle of fuselage, the thermal printer of fuselage roof installation and fuselage bottom installation, and the side of fuselage is provided with detection entrance and optical fiber interface for being placed in examined object；The front of fish tail handle is provided with the keyboard for information exchange, and the back side of fuselage is provided with the battery case for placing battery.Spectral analysis device provided by the invention is compact-sized, portable easy-to-use, blood of human body and urine can be analyzed and detected with immunochromatography technique principle based on fluorescence immunoassay, also the external fiber transmission that can be utilized quickly detects the fluorescence values in specified region, which solves the technical problem that existing analyzer mechanical transmission structure is complicated, light path devices utilization rate is low.
Description
Technical field
The present invention relates to fluorescence excitation and detection technique field more particularly to a kind of spectral analysis devices.
Background technique
Fluoroimmunoassay (FIA) is a kind of micro-analysis method developed in recent years, is most sensitive at present
One of trace analysis.The molecule of Cucumber can absorb energy and launch fluorescence, strong according to the spectrum and fluorescence of fluorescence
Degree, qualitative or quantitative method, referred to as fluorescence analysis are carried out to substance.Fluoroimmunoassay (FIA) have it is highly sensitive,
Selectivity is strong, needs the advantages that sample size is few and method is easy, and the Determination Limit of this method is usually 2~4 higher than spectrophotometry
The order of magnitude, in biochemical analysis using wide.
The general usual volume of fluorescent quantitative detector is larger at present, is not easy to portable use, therefore, limit its
It has inconvenient traffic, the development of under-developed area.Even if there is the product of some commercializations, there is also structure is complicated, poor reliability, essence
The disadvantages of degree is not high, and operation difficulty is big.Therefore, realize that instrument is portable and scalability is this field problem to be solved.
Summary of the invention
In view of the above problems, the present invention is to solve a kind of compact-sized, portable easy-to-use, the energy that provides spectral analysis device
Enough blood of human body and urine are analyzed and detected with immunochromatography technique principle based on fluorescence immunoassay, the outside that can also utilize
Optical fiber transmission quickly detects the fluorescence values in specified region, which solves existing analyzer mechanical transmission structure
Technical problem complicated, light path devices utilization rate is low.
A kind of spectral analysis device, which is characterized in that the thermal printer installed including fuselage, fuselage roof and fuselage bottom
The fish tail handle of portion's installation, in which: the fuselage is hollow rectangular parallelepiped structure, and the front of fuselage is provided with for showing data
Liquid crystal display, the side of fuselage are provided with the detection entrance for being placed in object to be checked and the optical fiber interface for connecting fiber optic bundle；Institute
The front for stating fish tail handle is provided with keyboard for information exchange, and the back side of fuselage is provided with the battery for placing battery
Box；
It further include the detection card for being inserted into detection entrance, the detection card includes detection plate, the test paper being placed in detection plate
The sample-adding pad being arranged between item and detection plate and test strips, in which: the recess that faces down of the detection plate, which is formed, holds examination
The concave station of paper slip, the back side of detection plate are provided with driving rack, and one end of detection plate forms hanging to interior 90 ° of folds after holding up
Platform, the other end of detection plate, which is holded up to be formed, is located at the top of sample-adding pad with the consistent support plate of hanging platform height, the hanging platform,
Well is offered on hanging platform；
The detection entrance extends to form sense channel to fuselage interior, and observation window is provided at the top of the sense channel, inspection
The bottom for surveying channel is provided with transmission notch；
The fuselage interior is additionally provided with the scanning transmission module for driving detection card mobile, and the scanning transmission module includes transmission
The microswitch of gear, the stepper motor with nutating gear rotation and induction detection card position, the transmission gear setting
It is moved repeatedly in the transmission notch by engaged transmission rack drives detection card, the microswitch is arranged in sense channel
Bottom；
The fuselage interior is additionally provided with the light source channel for generating light source, the light source channel be parallel to sense channel and
Light source including coaxial arrangement and with the light source optical filter that is located at light source side；
The fuselage interior be additionally provided with for receive feedback fluorescence light source feedback channel, the light source feedback channel perpendicular to
Sense channel and the first plano-convex lens including coaxial arrangement, detection optical filter, the second plano-convex lens, grating and spectral measurement
Device is provided with 45 ° of the inclination dichroic mirrors for light splitting in the axis intersection in light source feedback channel and light source channel, in which: institute
The top that the first plano-convex lens are mounted on the observation window is stated, the dichroic mirror is located at first plano-convex lens and detection is filtered
It is located at the ipsilateral of light source between mating plate and with light source optical filter, the spectral measurement device is used to measure the numerical value for receiving feedback light；
The fuselage interior further includes collection fibers, spectroscope and two groups of coupling assemblies, in which: the collection fibers include being used for
Import the vertical incidence optical fiber of light source, the horizontal reception optical fiber for exporting feedback light and for collecting the vertical incidence
Optical fiber and horizontal reception optical fiber summarize end；Coupling assembly is provided between the vertical incidence optical fiber and light source；The level
Coupling assembly is also equipped between reception optical fiber and dichroic mirror；The end of summarizing is mounted in optical fiber interface.
Further, the bottom of the sense channel is equipped with for reducing frictional force between sense channel and detection card
Roller bearing.
Further, the end of summarizing is by centrally located collimated optical fiber and evenly around in the parallel incidence
Multiple parallel reception optical fiber compositions of optical fiber.
Further, the end of vertically summarizing is by centrally located vertical incidence optical fiber and evenly around described vertical
Parallel reception optical fiber composition outside incident optical.
It further, further include the fiber optic bundle being detachably connected with optical fiber interface, described fiber optic bundle one end passes through connector
Connection collection fibers summarize end, and the fiber optic bundle other end connects optical fiber probe by connector.
Further, the coupling assembly includes the first convex lens, narrow band filter and the second convex lens of coaxial arrangement.
Further, detection band for capturing antigen antibody complex, free for being enriched with is provided on the test paper
The control band of fluorescent marker and the two dimensional code for record number.
Further, circuit board is additionally provided in the fuselage, the circuit board includes main control chip, amplification filtered electrical
Road, spectral measurement device, light source driving circuit and ID chip, power circuit, the A/D being respectively connected with the control chip are converted
Circuit, time sequence driving circuit, synchronous control circuit and motor-drive circuit, in which:
The main control chip is separately connected liquid crystal display, keyboard and thermal printer；
The main control chip passes sequentially through A/D conversion circuit, filtering and amplifying circuit obtains spectral measurement device information；
The spectral measurement device connects the time sequence driving circuit；
The main control chip passes sequentially through synchronous control circuit, light source driving circuit driving light source working；
The main control chip drives step motor control by clicking driving circuit；
The main control chip connects the positive and negative anodes in battery case by power circuit.
A kind of spectral analysis device of the invention has the advantages that this spectral analysis device is compact-sized, uses
The transmission scheme for detecting card backside rack gear and the cooperation of stepping motor rotating shaft gear, is substituted the motion platform scheme of transmission, reduces
The devices such as sliding block, ruler belt, guide rail, test strips frid, positioning device.Meanwhile the shape by designing detection plate, on the one hand
It can use the guidance detection card movement of sense channel shape, on the other hand can be convenient user and drip sample.This spectral analysis device is just
Take easy-to-use, analyzer includes thermal printer, battery case and liquid crystal display, can continue to make in the case where no extraneous power supply
With.This spectral analysis device scalability is strong, has external optical fiber interface, can by fiber optic bundle, connector and optical fiber probe
Reaction zone outside direct analyzer.Meanwhile by rationally designing optical path, common light source.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be in embodiment or description of the prior art
Required attached drawing is briefly described, it should be apparent that, the accompanying drawings in the following description is only some realities of the invention
Example is applied, it for those of ordinary skill in the art, without creative efforts, can also be according to these attached drawings
Obtain other attached drawings.
Fig. 1 is positive structure schematic of the invention；
Fig. 2 is side structure schematic diagram of the invention；
Fig. 3 is structure schematic diagram of the invention；
Fig. 4 is detection card schematic diagram of internal structure of the invention；
Fig. 5 is detection card positive structure schematic of the invention；
Fig. 6 is detection card backside structural schematic diagram of the invention；
Fig. 7 is schematic diagram of internal structure of the invention；
Fig. 8 is collection fibers structural schematic diagram of the invention；
Fig. 9 is collection fibers schematic diagram of internal structure of the invention；
Figure 10 is coupling assembly structural schematic diagram of the invention；
Figure 11 is fibre-optic connector structure schematic diagram of the invention；
Figure 12 is circuit connection diagram of the invention；
Figure 13 is light source driving circuit connection schematic diagram of the invention；
Figure 14 is filtering and amplifying circuit connection schematic diagram of the invention；
Figure 15 is A/D conversion circuit connection schematic diagram of the invention.
In figure: 1- fuselage, 101- liquid crystal display, 102- detection entrance, 103- optical fiber interface, 2- fish tail handle, 201- keyboard,
202- battery case, 3- thermal printer, 4- detection card, 401- detection plate, 402- test strips, 403- be loaded pad, 4011- concave station,
The hanging platform of 4012-, 4013- support plate, 4014- well, 4015- rack gear, 4021- detection band, 4022- control band, 4023- bis-
Tie up code, 5- sense channel, observation window at the top of 501-, 502- bottom driving notch, 6- scanning transmission module, 601- transmission gear,
602- stepper motor, 603- microswitch, 604- roller bearing, 7- light source channel, 701- light source, 702- light source optical filter, 8- light source
Feedback channel, the first plano-convex lens of 801-, 802- dichroic mirror, 803- detect optical filter, the second plano-convex lens of 804-, 805- light
Grid, 806- spectral measurement device, 9- circuit board, 10- coupling assembly, the first convex lens of 101-, 102- narrow band filter, 103- second
Convex lens, 11- collection fibers, 1101- vertical incidence optical fiber, the horizontal reception optical fiber of 1102-, 1103- summarize end, 12- spectroscope,
13- fiber optic bundle, 14- connector, 15- optical fiber probe.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figure 1, Figure 2, Figure 3 shows, a kind of spectral analysis device, including the thermal printing installed at the top of fuselage 1, fuselage 1
The fish tail handle 2 of machine 3 and the installation of 1 bottom of fuselage, in which: the fuselage 1 is hollow rectangular parallelepiped structure, the front setting of fuselage 1
There is the liquid crystal display 101 for showing data, the side of fuselage 1 is provided with the detection entrance 102 for being placed in object to be checked and is used for
Connect the optical fiber interface 103 of fiber optic bundle 13；The front of the fish tail handle 2 is provided with the keyboard 201 for information exchange, fuselage
1 back side is provided with the battery case 202 for placing battery；It further include the fiber optic bundle 13 being detachably connected with optical fiber interface 103,
End 1103 is summarized by the connection collection fibers 11 of connector 14 in described 13 one end of fiber optic bundle, and 13 other end of fiber optic bundle passes through
Connector 14 connects optical fiber probe 15.
Specifically, the present embodiment has external optical fiber interface 103, pass through fiber optic bundle 13, connector 14 and optical fiber probe 15
Reaction zone that can directly outside analyzer, is particularly suitable for the quick detection under ocean condition.Meanwhile by rationally designing light
Road, common light source and spectral measurement device.
As shown in figure 4, further including the detection card 4 for being inserted into detection entrance 102, the detection card 4 includes detection plate
401, the sample-adding pad 403 being arranged between the test strips 402 and detection plate 401 and test strips 402 being placed in detection plate 401,
Wherein: the recess that faces down of the detection plate 401 forms the concave station 4011 for holding test strips 402, as shown in Figure 5；Detection plate
401 back side is provided with rack gear 4015, as shown in Figure 6；It holds up rear fold inward and forms hanging platform in one end of detection plate 401
4012, the other end of detection plate 401 is holded up to be formed and the hanging 4012 consistent support plate 4013 of height of platform, the hanging platform 4012
Positioned at the top of sample-adding pad 403, well 4014 is offered on hanging platform 4012.
Specifically, the height of hanging platform 4012 and support plate 4013 should be consistent, and should be slightly below in sense channel 5
Highly, it can smoothly be moved and not shaken in sense channel 5 with guarantee detection card 4.It installs the bottom of the sense channel 5
There is the roller bearing 604 for reducing frictional force between sense channel 5 and detection card 4.In order to further decrease sense channel 5 and detection
Frictional force between card 4, can be using the material production detection card 4 and sense channel 5 of low-friction coefficient, such as nylon material.
Specifically, being provided with the detection band 4021 for capturing antigen antibody complex on the test paper, for being enriched with trip
Control band 4022 from fluorescent marker and for the two dimensional code 4023 of record number.
Test process example: will test buffer (the CRP antibody 1 for being fluorescently labeled substance markers) first and blood sample is mixed
It closes, CRP (antigen) reaction detected in CRP antibody 1 and blood in buffer forms antigen antibody complex.Work as mixture
After sample immerses test strips 402 by well 4014, test strips 402, capillarity can be run through and swum in test strips 402
Dynamic, the CRP antibody 2 that CRP antigen antibody complex is fixed on detection band 4021 is captured, and is enriched with or is trapped in test strips
On 402 detection band 4021, and free fluorescent marker can then cross detection band 4021 and be enriched at control band 4022.Blood
CRP in sample is more, detect band 4021 on compound gather more, detect band 4021 on fluorescent material content with
CRP concentration has certain corresponding relationship.
As shown in fig. 7, the detection entrance 102 forms sense channel 5 to 1 internal stretch of fuselage, the sense channel 5
Top is provided with observation window 501, and the bottom of sense channel 5 is provided with transmission notch 502；Band is additionally provided with inside the fuselage 1
The mobile scanning transmission module 6 of dynamic detection card 4, the scanning transmission module 6 include transmission gear 601, band nutating gear 601
The microswitch 603 of 4 positions of stepper motor 602 and induction detection card of rotation, the transmission gear 601 are arranged described
Detection card 4 is driven to move repeatedly by pinion rack 4015 in transmission notch 502, the setting of microswitch 603 is logical in detection
The bottom in road 5.
It should be noted that stepper motor 602 selects 6.5MM two-phase four-wire type stepper motor 602, according to the actual situation may be used
With 4 movement of driving detection card jointly of 2 groups of setting or multiple groups stepper motor 602.
Specifically, the outer profile of detection card 4 is slightly less than the outer profile of sense channel 5.When detection to be detected is blocked 4 by user
After being put into sense channel 5, the bottom rack gear of detection card 4 is engaged with the transmission gear 601 of 5 bottom of sense channel, when detection card 4 is complete
After being put into sense channel 5 entirely, detection card 4 will depress the microswitch 603 of 5 bottom of sense channel, and microswitch 603 will touch at this time
Photos and sending messages feedback is to main control chip, after main control chip obtains the information that detection card 4 is fully inserted into, starts to control stepper motor 602
Forward and backward is to realize that detection card 4 is repeatedly scanned with.
As shown in fig. 7, the light source channel 7 for generating light source is additionally provided with inside the fuselage 1, the light source channel 7
Be parallel to sense channel 5 and light source 701 including coaxial arrangement and with the light source optical filter 702 that is located at 701 side of light source；
Be additionally provided with inside the fuselage 1 for receive feedback fluorescence light source feedback channel 8, the light source feedback channel 8 perpendicular to
Sense channel 5 and the first plano-convex lens 801 including coaxial arrangement, detection optical filter 803, the second plano-convex lens 804, grating
805 and spectral measurement device 806,45 ° of inclination are provided with for being divided in the axis intersection in sense channel 5 with 701 channel 7 of light source
Dichroic mirror 802, in which: first plano-convex lens 801 are mounted on the top of the observation window 501, the dichroic mirror 802
It is located at the same of light source 701 between first plano-convex lens 801 and detection optical filter 803 and with light source optical filter 702
Side, the spectral measurement device 806 are used to measure the numerical value for receiving feedback light；Further include collection fibers 11 inside the fuselage 1, divide
Light microscopic 12 and two groups of coupling assemblies 10, in which: the collection fibers 11 include the vertical incidence optical fiber for importing light source 701
1101, for exporting the horizontal reception optical fiber 1102 of feedback light and for collecting the vertical incidence optical fiber 1101 and level
Reception optical fiber 1102 summarizes end 1103；Coupling assembly 10 is provided between the vertical incidence optical fiber 1101 and light source 701；Institute
It states and is also equipped with coupling assembly 10 between horizontal reception optical fiber 1102 and dichroic mirror 802；The end 1103 of summarizing is mounted on optical fiber
In interface 103.
Specifically, above-mentioned grating 805 is aperture grating 805.
Specifically, horizontal reception optical fiber 1102 and coupling assembly 10 are coaxially arranged, vertical incidence optical fiber 1101 and coupling systems
Part 10, light source 701 are coaxially arranged, and horizontal reception optical fiber 1102 and the extension line of vertical incidence optical fiber 1101 are mutually perpendicular to.
The specific structure of collection fibers 11 is as shown in Figure 8, Figure 9, since the emergent light spot of vertical incidence optical fiber 1101 is round
Symmetrically, the fluorescence hot spot obtained is also round symmetrically.Therefore, the fiber optic bundle 13 of symmetric distributed is used to receive reflective light intensity to mention
Height receives the efficiency and sensitivity of light, and in order to reduce the loss in transmission process, the optical fiber used is shorter, about 60-80cm.
Light source 701 and the simplest method of fiber coupling are direct-couplings, i.e., the direct alignment light source 701 of optical fiber planar end surface
Light-emitting surface.This method is simple and convenient, but coupling efficiency is low.If the numerical aperture of optical fiber be 0.26, it is direct-coupled most
High efficiency is only 6.7%.By placing convergent lens between light source 701 and fiber end face, change the luminous direction of xenon flash lamp
Or the acceptance angle of optical fiber, coupling efficiency can be improved, as shown in Figure 10, coupling assembly 10 includes the first convex lens of coaxial arrangement
Mirror, narrow band filter and the second convex lens, using coupled lens, on the one hand to exciting light beam collimation, to improve the note of probe
Enter efficiency；On the other hand it can collect fluorescence signal, to improve the utilization rate of fluorescence signal.Optical fiber connector 14 in the present embodiment
Structural schematic diagram as indicated at 11.
Specifically, connector 14 can connect multimode plastic optical fiber beam 13, single mode optical fiber and optical fiber probe 15, to generate
The transmission road of fluorescence signal and exciting light.Specifically, fiber optic bundle 13 can according to circumstances use the small-sized flexible optical cable of armouring.
It should be noted that the course of work in 701 channel 7 of light source and light source feedback channel is as follows:
1, it is analyzed using 4 measurement of detection card
Light source 701 is lighted when detection, the light splitting dichroic mirror 802 placed after the collimated optical filter of exciting light by 45 °, then by gathering
The first plano-convex lens of coke/collimation 801 focus on 402 surface of test strips.At this point, stepper motor 602 drives test strips 402 steadily to move
It is dynamic.Excite the detection zone in optical scanning test strips 402.What the fluorescent material in test strips 402 in detection zone was inspired
Fluorescence is directional light by the first plano-convex lens 801 collimation, filters out veiling glare through dichroic mirror 802 and detection optical filter 803, then by second
Plano-convex lens 804 focus on the burnt aperture of copolymerization.It is detected by the light of diaphragm by spectral measurement device 806 and is converted to telecommunications
Number, concentration of the electric signal through handling with can be converted into tested sample after analytical calculation.
2, it is measured and is analyzed using optical fiber probe 15
Light source 701 is lighted when detection, and the coupled component 10 of exciting light enters vertical incidence optical fiber 1101, then successively by summarizing end
1103, connector 14, fiber optic bundle 13, connector 14 enter in optical fiber probe 15, and exciting light is issued in optical fiber probe 15；Detection
The fluorescence that fluorescent material in region is inspired again successively by 15 female connector 14 of optical fiber probe, fiber optic bundle 13, connector 14,
Summarize 1103 feedback of end to horizontal reception optical fiber 1102, is changed at this time by the fluorescence that horizontal reception optical fiber 1102 is fed back through spectroscope 12
Become incident direction, detected by the light of diaphragm by spectral measurement device 806 and be converted to electric signal, which is handled and analyzed
The concentration of tested sample can be converted into after calculating.
As shown in figure 12, circuit board is additionally provided in the fuselage 1, the circuit board includes main control chip, amplification filtering
Circuit, spectral measurement device 806,701 driving circuit of light source and the ID chip being respectively connected with the control chip, power supply electricity
Road, A/D conversion circuit, time sequence driving circuit, synchronous control circuit and motor-drive circuit, in which: the main control chip difference
Connect liquid crystal display 101, keyboard 201 and thermal printer 3；The main control chip passes sequentially through A/D conversion circuit, amplification filtered electrical
Road obtains 806 information of spectral measurement device；The spectral measurement device 806 connects the time sequence driving circuit；The main control chip according to
It is secondary to drive light source 701 to work by synchronous control circuit, 701 driving circuit of light source；The main control chip is by clicking driving electricity
Road drives stepper motor 602 to control；The main control chip connects the positive and negative anodes in battery case 202 by power circuit.
In the present embodiment, light source 701 can be hernia lamp source 701.
Figure 13 is 701 driving circuit connection schematic diagram of light source of the invention, is filled by power supply to storage capacitor C
Electricity, pressure stabilizing control element is started to work when the voltage when its both ends reaches preset value, keeps its invariable.When needing to glisten,
Trigger circuit is controlled by interface circuit by main control chip, capacitor E generates heavy current, triggered arteries and veins through resistance Rl repid discharge
The high electric field pulse that transformer generates volts up to ten thousand in its secondary grade is rushed, makes the gas ionization in xenon lamp, substantially reduces the internal resistance of lamp,
The a large amount of electric energy stored in storage capacitor C are released by xenon flash lamp in a very short period of time, to generate extremely strong sudden strain of a muscle
Light.
The present embodiment is reversely amplified using CA3450 operational amplifier, although CA3450 is completed to the anti-of signal
To amplification, but in its output signal, noise is bigger, and filter circuit is needed to filter out noise, improves signal quality.Since it is desired that
The signal frequency arrived is relatively low, so selecting common voltage controlled voltage source second-order low-pass filter to filter out noise, as shown in figure 14.
The present embodiment sampling and holding stage are mainly realized by sampling/retainer, and quantify main with coding stage
It is to be realized by A/D converter, the A/D converter of the present embodiment selects MAX120.Gain adjustment and dual polarity bias tune
It is whole, it is realized by the potentiometer RP2 and RP1 in Figure 15, biasing adjustment should be prior to gain adjustment in adjustment.Between the two adjustment
May have it is some influence each other, need to adjust repeatedly.The adjustment of biasing and gain is the subdivision to A/D conversion, it is therefore intended that is mentioned
The precision of high A/D.
The spectral measurement device 806 of the present embodiment can use TCD1200D type CCD or silicon photocell.
C reactive protein is detected using the present embodiment, within the scope of the reagent concentration of 2.00~30.00mg/L, every
The same fluorescent reagent that 2mg/L chooses concentration level carries out test analysis, every kind of concentration level test be averaged for 4 times for
Final result.Linear regression analysis, resulting equation of linear regression are carried out to the result measured are as follows: y=0.762x+0.215,
Middle coefficient R2=0.987, the above result shows that instrument is in 2.00~30.00mg/L concentration range with good linear
Response characteristic.
Spectral analysis device provided in this embodiment is compact-sized, portable easy-to-use, based on fluorescence immunoassay and immunochromatography skill
Art principle is analyzed and is detected to blood of human body and urine, solves existing analyzer mechanical transmission structure complexity, optical path device
The low technical problem of part utilization rate.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (8)
1. a kind of spectral analysis device, which is characterized in that the thermal printer and fuselage bottom installed including fuselage, fuselage roof
The fish tail handle of installation, in which: the fuselage is hollow rectangular parallelepiped structure, and the front of fuselage is provided with the liquid for showing data
Crystalline substance screen, the side of fuselage is provided with the detection entrance for being placed in object to be checked and the optical fiber interface for connecting fiber optic bundle；It is described
The front of fish tail handle is provided with the keyboard for information exchange, and the back side of fuselage is provided with the battery case for placing battery；
It further include the detection card for being inserted into detection entrance, the detection card includes detection plate, the test paper being placed in detection plate
The sample-adding pad being arranged between item and detection plate and test strips, in which: the recess that faces down of the detection plate, which is formed, holds examination
The concave station of paper slip, the back side of detection plate are provided with driving rack, and one end of detection plate forms hanging to interior 90 ° of folds after holding up
Platform, the other end of detection plate, which is holded up to be formed, is located at the top of sample-adding pad with the consistent support plate of hanging platform height, the hanging platform,
Well is offered on hanging platform；
The detection entrance extends to form sense channel to fuselage interior, and observation window is provided at the top of the sense channel, inspection
The bottom for surveying channel is provided with transmission notch；
The fuselage interior is additionally provided with the scanning transmission module for driving detection card mobile, and the scanning transmission module includes transmission
The microswitch of gear, the stepper motor with nutating gear rotation and induction detection card position, the transmission gear setting
It is moved repeatedly in the transmission notch by engaged transmission rack drives detection card, the microswitch is arranged in sense channel
Bottom；
The fuselage interior is additionally provided with the light source channel for generating light source, the light source channel be parallel to sense channel and
Light source including coaxial arrangement and with the light source optical filter that is located at light source side；
The fuselage interior be additionally provided with for receive feedback fluorescence light source feedback channel, the light source feedback channel perpendicular to
Sense channel and the first plano-convex lens including coaxial arrangement, detection optical filter, the second plano-convex lens, grating and spectral measurement
Device is provided with 45 ° of the inclination dichroic mirrors for light splitting in the axis intersection in light source feedback channel and light source channel, in which: institute
The top that the first plano-convex lens are mounted on the observation window is stated, the dichroic mirror is located at first plano-convex lens and detection filters
It is located at the ipsilateral of light source between piece and with light source optical filter, the spectral measurement device is used to measure the numerical value for receiving feedback light；
The fuselage interior further includes collection fibers, spectroscope and two groups of coupling assemblies, in which: the collection fibers include being used for
Import the vertical incidence optical fiber of light source, the horizontal reception optical fiber for exporting feedback light and for collecting the vertical incidence
Optical fiber and horizontal reception optical fiber summarize end；Coupling assembly is provided between the vertical incidence optical fiber and light source；The level
Coupling assembly is also equipped between reception optical fiber and dichroic mirror；The end of summarizing is mounted in optical fiber interface.
2. spectral analysis device according to claim 1, which is characterized in that the bottom of the sense channel, which is equipped with, to be used for
Reduce the roller bearing of frictional force between sense channel and detection card.
3. spectral analysis device according to claim 2, which is characterized in that the end of summarizing is entered in parallel by centrally located
It penetrates optical fiber and is formed evenly around multiple parallel reception optical fibers in the collimated optical fiber.
4. spectral analysis device according to claim 3, which is characterized in that hung down by centrally located at the end of vertically summarizing
Straight incident optical and evenly around the parallel reception optical fiber composition in the vertical incidence outer fiber.
5. spectral analysis device according to claim 4, which is characterized in that further include being detachably connected with optical fiber interface
End is summarized by connector connection collection fibers in fiber optic bundle, described fiber optic bundle one end, and the fiber optic bundle other end passes through connection
Device connects optical fiber probe.
6. spectral analysis device according to claim 5, which is characterized in that the coupling assembly includes the of coaxial arrangement
One convex lens, narrow band filter and the second convex lens.
7. spectral analysis device according to claim 6, which is characterized in that be provided on the test paper for capturing antigen
The detection band of antibody complex, the two dimensional code controlled with and for record number for being enriched with free fluorescent marker.
8. spectral analysis device according to claim 7, which is characterized in that be additionally provided with circuit board, institute in the fuselage
Circuit board is stated to include main control chip, filtering and amplifying circuit, spectral measurement device, light source driving circuit and divide with the control chip
Not Xiang Lian ID chip, power circuit, A/D conversion circuit, time sequence driving circuit, synchronous control circuit and motor-drive circuit,
Wherein:
The main control chip is separately connected liquid crystal display, keyboard and thermal printer；
The main control chip passes sequentially through A/D conversion circuit, filtering and amplifying circuit obtains spectral measurement device information；
The spectral measurement device connects the time sequence driving circuit；
The main control chip passes sequentially through synchronous control circuit, light source driving circuit driving light source working；
The main control chip drives step motor control by clicking driving circuit；
The main control chip connects the positive and negative anodes in battery case by power circuit.
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| CN201810248659.7A CN110174509A (en) | 2018-03-24 | 2018-03-24 | A kind of spectral analysis device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810248659.7A CN110174509A (en) | 2018-03-24 | 2018-03-24 | A kind of spectral analysis device |
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| CN110174509A true CN110174509A (en) | 2019-08-27 |
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| CN201810248659.7A Pending CN110174509A (en) | 2018-03-24 | 2018-03-24 | A kind of spectral analysis device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112129749A (en) * | 2020-07-11 | 2020-12-25 | 海南蓝瑞科技有限公司 | Automatic urine detection device for closestool |
| CN113640508A (en) * | 2021-07-19 | 2021-11-12 | 安徽桐康医疗科技股份有限公司 | Detection plate for fluorescence immunoassay quantitative analyzer |
| CN114200131A (en) * | 2021-12-13 | 2022-03-18 | 成都云芯医联科技有限公司 | Detection system for luminous characteristics of luminous pearl-like material and preparation method of matched detection test paper |
| CN117929345A (en) * | 2024-03-22 | 2024-04-26 | 北京万泰生物药业股份有限公司 | Handheld detection device and fluorescence detection system |
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| CN102253015A (en) * | 2011-03-23 | 2011-11-23 | 中国科学院上海光学精密机械研究所 | Embedded immunochromatography fluorescence detection system and detection method |
| CN207908516U (en) * | 2018-03-24 | 2018-09-25 | 西安鲲创科技发展有限公司 | A kind of spectral analysis device |
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| CN102253015A (en) * | 2011-03-23 | 2011-11-23 | 中国科学院上海光学精密机械研究所 | Embedded immunochromatography fluorescence detection system and detection method |
| CN207908516U (en) * | 2018-03-24 | 2018-09-25 | 西安鲲创科技发展有限公司 | A kind of spectral analysis device |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112129749A (en) * | 2020-07-11 | 2020-12-25 | 海南蓝瑞科技有限公司 | Automatic urine detection device for closestool |
| CN113640508A (en) * | 2021-07-19 | 2021-11-12 | 安徽桐康医疗科技股份有限公司 | Detection plate for fluorescence immunoassay quantitative analyzer |
| CN113640508B (en) * | 2021-07-19 | 2024-02-27 | 安徽桐康医疗科技股份有限公司 | Detection plate for fluorescent immunity quantitative analyzer |
| CN114200131A (en) * | 2021-12-13 | 2022-03-18 | 成都云芯医联科技有限公司 | Detection system for luminous characteristics of luminous pearl-like material and preparation method of matched detection test paper |
| CN114200131B (en) * | 2021-12-13 | 2023-08-15 | 成都云芯医联科技有限公司 | Detection system for luminous characteristics of luminous beads and preparation method of matched detection test paper |
| CN117929345A (en) * | 2024-03-22 | 2024-04-26 | 北京万泰生物药业股份有限公司 | Handheld detection device and fluorescence detection system |
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