CN109298179A - A kind of immunochromatography detection system and its Background Recognition method - Google Patents
A kind of immunochromatography detection system and its Background Recognition method Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The present invention relates to a kind of immunochromatography detection system and its Background Recognition methods.Including main control unit, optical measurement unit, background acquisition unit；Main control unit includes master cpu and the driving circuit connecting with the master cpu, display module, the transmission device that optical measurement unit includes optical detection apparatus, is connect with driving circuit, background acquisition unit includes the background signal input module connecting with optical detection apparatus, the conditioning filtration module connecting with background signal input module, the conditioning amplification module connecting with conditioning filtration module, and conditioning amplification module is also connect with master cpu.The present invention improves the detection range and precision of immunochromatography detection system；Meanwhile it is so very big that reduce influence caused by clinically sample to be tested physicochemical property difference；The present invention is practical, can be widely applied to the fields such as detection, food safety detection by medicine bed, and effective solution immunochromatography instrument is because caused by background baseline problem the shortcomings that instrument performance reduction.
Description
Technical field
The present invention relates to a kind of immunochromatography detection system and its Background Recognition methods.
Background technique
Patent (application number: 201810134327.6) proposing a kind of dry type immunochromatography detector and its detection method,
In include fluorescence detection mechanism, movement mechanism, sweep mechanism, decision mechanism, switching mechanism, information acquisition module and master control mould
Block etc..The design realizes the judgement of T, C line and T/C area ratio, quantitative detection concentration of specimens by software algorithm.Patent (application
Number: 201711404592.3) propose a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system, including optical system, number
According to Transmission system and data processing system.Detection system passes through the collected high-resolution fluorescence strip image information of microscope
Image segmentation algorithm is partitioned into T line, C line and background area in strip image.Patent (application number: 201610835153.7) mentions
A kind of quantitative detection calculation method based on fluorescence immune chromatography technology out is successively irradiated by LED light source, at optical filter optical filtering
Reason, Electric signal processing, AD conversion processing, filtering algorithm processing, baseline fitting, peak-seeking processing, calculates T/ at photodetector detection
C area ratio, the process for calculating concentration, to be judged by the concentration results calculated.Using minimum in background selection
Two multiply the selected background baseline of fitting, quantitative detection sample to be tested concentration.
There are problem or defects for above-mentioned technology:
Clinically there are different degrees of differences on physicochemical property for sample to be tested, lead to immunity-chromatography test strip detection zone background
Unstability of base line is fixed, influences the accuracy of testing result.Therefore, accurately choosing background baseline is the premise for realizing quantitative detection.
In existing immunochromatography detection system, background baseline choosing method is as follows: 1, selecting background baseline by baseline fitting；2, pass through
Second-order differential method determines knee of characteristic, and using the corresponding baseline value of inflection point as background baseline；3, it is risen by immune strip
Beginning position continuous acquisition is averaged, as background baseline.First two method is off-line data processing method, needs the fortune by PC
Calculation ability, it is difficult to realize these functions on quick detection device at the scene；A kind of last method can only weaken in actually detected
Accidental error, it is then helpless for systematic error existing for traditional baseline choosing method itself.
Summary of the invention
The purpose of the present invention is to provide a kind of immunochromatography detection system and its Background Recognition methods, improve immune layer
Analyse the detection range and precision of detection system；Meanwhile it is so very big that reduce caused by clinically sample to be tested physicochemical property difference
It influences.
To achieve the above object, the technical scheme is that a kind of immunochromatography detection system, including main control unit,
Optical measurement unit, background acquisition unit；The main control unit include master cpu and the driving circuit being connect with the master cpu,
Display module, the optical measurement unit includes optical detection apparatus, the transmission device that connect with the driving circuit, the back
Scape acquisition unit includes the background signal input module connecting with the optical detection apparatus and the background signal input module
The conditioning filtration module of connection, the conditioning amplification module being connect with the conditioning filtration module, the conditioning amplification module also with
The master cpu connection；The optical detection apparatus includes light source, the first lens, dichroscope, cylindrical mirror, optical filter, second
The light of lens, photoelectric sensor, light source activation is totally reflected after the first lens focus by dichroscope, and after through cylindrical mirror shaping
It is changed into rectangular light spot, and acts on and be placed in the immunity-chromatography test strip surface to be measured on the transmission device, immunochromatography to be measured
The optical signal of strip surface reflection is converted to telecommunications by the second lens focus to photoelectric sensor through dichroscope, optical filter
Number.The chromogenic line adjoining dimensions of the rectangular light spot size and immunity-chromatography test strip to be measured.Fig. 4 shows optical facilities of the present invention
Irradiate the hot spot schematic diagram of strip detection window.Enabling spot width is L, and detection bandwidth degree is LT, control bandwidth degree is LC, detection
Distance is L between band and control bandTC, when designing hot spot, its width L is made to level off to LTWith LC, and it is much smaller than LTC, i.e.,, reasonable light spot shape and size detect band and control band when making optical facilities scanning strip
Optical signal does not generate interference and is conducive to the division of background area, improves detection accuracy.
The present invention also provides a kind of Background Recognition method based on a kind of immunochromatography detection system described above, packets
Include following steps:
Step S1, when immunity-chromatography test strip to be measured measures, light source is opened, by dichroscope after the first lens focus of light source activation light
Total reflection, and after through cylindrical mirror shaping be changed into rectangular light spot, act on immunity-chromatography test strip surface to be measured；
Step S2, master cpu control transmission device moves back and forth, and makes rectangular light spot in the detection on immunity-chromatography test strip surface to be measured
Window scans back and forth, and will test the optical signal on window by dichroscope, optical filter, by the second lens focus to photoelectric transfer
Sensor is converted to electric signal；
Step S3, background signal input module receives the electric signal of photoelectric sensor transmission, is transmitted to conditioning filtering mould
Block, conditioning amplification module are handled in real time, and are finally transferred to master cpu and are carried out A/D conversion；
Step S4, the signal after master cpu is converted according to A/D, identifies background area, and the sweep length of the background area is made
Be control variable storage in master cpu, by adjusting transmission device stepper motor progressive step away from making each beam spot scans
Dynamic range control background area theoretically when start sampling processing, then pass through strip movement speed, strip window
Length and facula position know method for distinguishing, find stable background area, and using determining background area sampled result as most
Whole background baseline, finally carries out mean value computation by algorithm, the baseline value as immunity-chromatography test strip background area to be measured.
Compared to the prior art, the invention has the following advantages: the present invention improves immunochromatography detection system
Detection range and precision；Meanwhile it is so very big that reduce influence caused by clinically sample to be tested physicochemical property difference；The present invention is real
It is strong with property, can be widely applied to the fields such as detection, food safety detection by medicine bed, effective solution immunity-chromatography test strip because
The shortcomings that instrument performance caused by baseline problem reduces.
Detailed description of the invention
Fig. 1 is the optical texture of immunochromatography detection system.
Fig. 2 is the area allocation diagram of immunochromatography optical signal sampling curve.
Fig. 3 is immunochromatography detection system functional block diagram of the present invention.
Fig. 4 is the hot spot schematic diagram of optical facilities irradiation strip detection window of the present invention.
Specific embodiment
With reference to the accompanying drawing, technical solution of the present invention is specifically described.
The present invention provides a kind of immunochromatography detection system, including the acquisition of main control unit, optical measurement unit, background are single
Member；The main control unit includes master cpu and the driving circuit connecting with the master cpu, display module, the optical measurement list
The transmission device that member includes optical detection apparatus, connect with the driving circuit, the background acquisition unit include and the light
Learn detection device connection background signal input module, connect with the background signal input module conditioning filtration module, and
The conditioning amplification module of the conditioning filtration module connection, the conditioning amplification module are also connect with the master cpu；The light
Learning detection device includes light source, the first lens, dichroscope, cylindrical mirror, optical filter, the second lens, photoelectric sensor, and light source swashs
The light of hair is totally reflected after the first lens focus by dichroscope, and after through cylindrical mirror shaping be changed into rectangular light spot, and act on
In being placed in the immunity-chromatography test strip surface to be measured on the transmission device, the optical signal of immunity-chromatography test strip surface reflection to be measured is passed through
Dichroscope, optical filter are converted to electric signal by the second lens focus to photoelectric sensor.The rectangular light spot size with to
Survey the chromogenic line adjoining dimensions of immunity-chromatography test strip.
The present invention also provides a kind of Background Recognition method based on a kind of immunochromatography detection system described above, packets
Include following steps:
Step S1, when immunity-chromatography test strip to be measured measures, light source is opened, by dichroscope after the first lens focus of light source activation light
Total reflection, and after through cylindrical mirror shaping be changed into rectangular light spot, immunity-chromatography test strip surface to be measured；
Step S2, master cpu control transmission device moves back and forth, and makes rectangular light spot in the detection on immunity-chromatography test strip surface to be measured
Window scans back and forth, and will test the optical signal on window by dichroscope, optical filter, by the second lens focus to photoelectric transfer
Sensor is converted to electric signal；
Step S3, background signal input module receives the electric signal of photoelectric sensor transmission, is transmitted to conditioning filtering mould
Block, conditioning amplification module are handled in real time, and are finally transferred to master cpu and are carried out A/D conversion；
Step S4, the signal after master cpu is converted according to A/D, identifies background area, and the sweep length of the background area is made
Be control variable storage in master cpu, by adjusting transmission device stepper motor progressive step away from making each beam spot scans
Dynamic range control (non-optimal) background area theoretically when start sampling processing, then by strip movement speed,
Strip length of window and facula position know method for distinguishing, find stable background area, and determining background area is sampled knot
Fruit finally carries out mean value computation by algorithm as final background baseline, the baseline as immunity-chromatography test strip background area to be measured
Value.
The following are specific implementation processes of the invention.
As shown in figure 3, a kind of immunochromatography detection system of the invention, mainly acquires mould by optical measurement module, background
Block, main control module are constituted.It is as shown in Figure 1 the optical texture of immunochromatography detection system.When strip measures, light source is opened, and is swashed
It shines and is totally reflected after over-focusing by dichroscope, be changed into rectangular light spot through cylindrical mirror shaping, and act on strip table to be measured
Face.The transmission device that the master cpu control of subsequent main control module is loaded with strip moves back and forth, and width x length is made to adjust suitable square
Shape hot spot is scanned back and forth in the detection window of strip to be measured, and be will test the optical signal on window and passed through dichroscope and optical filtering
Piece focuses on optical sensor by focus lamp, is ultimately converted to electric signal.By the optical telecommunications for analyzing beam spot scans detection zone
Number regularity of distribution realizes background area Dynamic Recognition.
The working principle of present system is as follows:
Since the size that the chromogenic line in strip to be measured shows is rectangular strip, to make full use of front end light path system to detection window
The extraction of oral examination bars amount, for the emergent light of light source after optical path, the hot spot for focusing on strip detection window should be designed as rectangle
The size of hot spot, rectangular light spot is designed according to strip detection window size.When being detected, master cpu is loaded with by control
The transmission device of strip moves back and forth, and scans back and forth rectangular light spot in the window of strip to be measured, and by the photosignal of scanning
Background acquisition is inputted by receiver (photoelectric sensor of Fig. 1) to be handled in real time with conditioning circuit, and is finally transferred to master
It controls CPU and carries out A/D conversion.As shown in Fig. 2, being the area allocation diagram of immunochromatography optical signal sampling curve, due to detection window
Lower edges region reflection coefficient it is different from background area, cause received background signal to there is drift, therefore cannot function as carrying on the back
The region of scape selection.Similarly, the T line region and C line region in detection window should be excluded, then should be in remaining Region Theory
For background area.First using the sweep length of the background area as control variable storage in CPU, by accurately controlling stepping
The progressive step of motor is away from making to start to adopt when the dynamic range control of each beam spot scans (non-optimal) background area theoretically
Sample processing, then denoising is filtered to sampled data, secondly by strip movement speed, strip length of window and hot spot position
Knowledge method for distinguishing is set, finds stable background area, and using determining background area sampled result as final background baseline, most
Mean value computation is carried out by algorithm afterwards, the baseline value as strip background area.
Usage mode of the invention:
The principle of the present invention block diagram is as shown in Figure 3.After being inserted into immunity-chromatography test strip, measurement button is pressed, master cpu receives life
It enables, begins through the fixed step pitch of driving circuit control transmission device traveling, realize the accurate positionin and detection in background area,
And signal is transmitted to background Acquisition Circuit by optical facilities.Filtering, conditioning amplification input CPU carry out A/D conversion after conditioning, and
It is final to determine background baseline.After beam spot scans, CPU carries out according to the change in optical signal in scanning process characteristic quantity and mentions
It takes and is shown on LCD LCD screen.
The above are preferred embodiments of the present invention, all any changes made according to the technical solution of the present invention, and generated function is made
When with range without departing from technical solution of the present invention, all belong to the scope of protection of the present invention.
Claims (2)
1. a kind of immunochromatography detection system, which is characterized in that including main control unit, optical measurement unit, background acquisition unit；
The main control unit includes master cpu and the driving circuit connecting with the master cpu, display module, the optical measurement unit
Including optical detection apparatus, the transmission device connecting with the driving circuit, the background acquisition unit includes and the optics
The background signal input module of detection device connection, the conditioning filtration module being connect with the background signal input module and institute
The conditioning amplification module of conditioning filtration module connection is stated, the conditioning amplification module is also connect with the master cpu；The optics
Detection device includes light source, the first lens, dichroscope, cylindrical mirror, optical filter, the second lens, photoelectric sensor, light source activation
Light be totally reflected after the first lens focus by dichroscope, and after through cylindrical mirror shaping be changed into rectangular light spot, and act on
It is placed in the immunity-chromatography test strip surface to be measured on the transmission device, the optical signal of immunity-chromatography test strip surface reflection to be measured is through two
Electric signal is converted to by the second lens focus to photoelectric sensor to Look mirror, optical filter；The rectangular light spot size with it is to be measured
The chromogenic line adjoining dimensions of immunity-chromatography test strip.
2. a kind of based on a kind of Background Recognition method of immunochromatography detection system described in claim 1, which is characterized in that packet
Include following steps:
Step S1, when immunity-chromatography test strip to be measured measures, light source is opened, by dichroscope after the first lens focus of light source activation light
Total reflection, and after through cylindrical mirror shaping be changed into rectangular light spot, act on immunity-chromatography test strip surface to be measured；
Step S2, master cpu control transmission device moves back and forth, and makes rectangular light spot in the detection on immunity-chromatography test strip surface to be measured
Window scans back and forth, and will test the optical signal on window by dichroscope, optical filter, by the second lens focus to photoelectric transfer
Sensor is converted to electric signal；
Step S3, background signal input module receives the electric signal of photoelectric sensor transmission, is transmitted to conditioning filtering mould
Block, conditioning amplification module are handled in real time, and are finally transferred to master cpu and are carried out A/D conversion；
Step S4, the signal after master cpu is converted according to A/D, identifies background area, and the sweep length of the background area is made
Be control variable storage in master cpu, by adjusting transmission device stepper motor progressive step away from making each beam spot scans
Dynamic range control background area theoretically when start sampling processing, then pass through strip movement speed, strip window
Length and facula position know method for distinguishing, find stable background area, and using determining background area sampled result as most
Whole background baseline, finally carries out mean value computation by algorithm, the baseline value as immunity-chromatography test strip background area to be measured.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112053299A (en) * | 2020-08-27 | 2020-12-08 | 杭州安旭生物科技股份有限公司 | Image processing method based on colloidal gold immunochromatography |
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