CN107561073A - A kind of full spectrum ELIASA for being designed with eight passage cell holders - Google Patents
A kind of full spectrum ELIASA for being designed with eight passage cell holders Download PDFInfo
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- CN107561073A CN107561073A CN201710853786.5A CN201710853786A CN107561073A CN 107561073 A CN107561073 A CN 107561073A CN 201710853786 A CN201710853786 A CN 201710853786A CN 107561073 A CN107561073 A CN 107561073A
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Abstract
The present invention relates to a kind of full spectrum ELIASA for being designed with eight passage cell holders, including tungsten lamp, concave mirror, monochrometer, fibre bundle, fiber bench, ELISA Plate and photoelectric sensor, the all-wave length light that tungsten lamp is sent enters monochrometer after concave mirror deflection angle, the monochromatic light of desired wavelength can be adjusted out rapidly in monochrometer, the monochromatic light is that ten uniform light splitting are fine by fibre bundle, wherein eight are main fiber, one is that spectrophotometric optical fiber is used to connect spectrophotometric module, and another is used to connect monitoring optical fiber head for monitoring optical fiber.This ELIASA can accurately detect the concentration value of solution soluble thing to be measured, there is repeatability and the degree of accuracy well, and reference instrument can be used as to configure the detection unit and research institution etc. such as each hospital, health and epidemic prevention station；It can realize that all-wave length examines material to be checked, meet the needs that many kinds of substance is examined；Instrument buying expenses can be saved and save the instrument floor space in laboratory.
Description
Technical field
The invention belongs to medical test or chemical analysis field, is related to ELIASA, especially one kind and is designed with eight passage ratios
The full spectrum ELIASA of color ware seat.
Background technology
ELIASA in field of medical examination at present, it is substantially based on light of the interferometric filter as monochromatic light discrete device
Electric colorimetric instrument.The Cleaning Principle of interference filter chip ELIASA is：The light irradiation that tungsten lamp is sent selects into interferometric filter
Disk, disk is selected by rotational interference optical filter, the interferometric filter of fixed wave length can be rotated to, tungsten lamp incident light is dry by this
Relate to the monochromatic light that fixed wave length has just been waited until after optical filter.Monochromatic light passes through optical fibre light splitting, forms eight energy uniformly small light
Fibre, eight small optical fiber are fixed on fiber fixed seat, and the uniform light of eight beams projects from the lens on fiber fixed seat, is transmitted through
ELISA Plate, some light the liquid that remaining light transmission goes out in ELISA Plate, are radiated at by the liquid absorption to be checked in ELISA Plate
In photoelectric sensor, optical signal is changed into electric signal in photoelectric sensor.The electric signal obtained by analyzing photoelectric sensor
It is strong and weak, it is possible to which that analysis draws the strength of fluid in ELISA Plate.The problem of it is present is as follows：1st, interference filter chip enzyme is being used
, it is necessary to first recall main inspection wavelength when mark instrument measurement testing concentration, put on ELISA Plate plus artificial zero check, Ran Houjian
Solution to be checked is tested, then recalls secondary inspection wavelength, ELISA Plate will also be put plus artificial zero check, examine solution to be checked again, a kind of
The inspection of solution needs multi-pass operation, easily because the error of operating personnel causes the deviation of testing result；2nd, interferometric filter enzyme
The optical filter for marking instrument sets limited amount, and each interferometric filter wavelength is fixed, and general instrument is not over 8, main inspection ripple
The long and secondary selection typically each 4 for examining wavelength, it is impossible to meet the inspection needs of a variety of kind of inspection；3rd, interferometric filter enzyme mark
It is usually 8~10nm that instrument half-wavelength is very wide, and monochromatic wavelength variation range is big, and wavelength is inaccurate, the inspection degree of accuracy of instrument
Difference；4th, many layer films are coated with interferometric filter, the film of these multilayers prevents the biography of monochromatic light energy to a certain extent
Broadcast；For interferometric filter ELIASA in order to obtain the monochromatic light of sufficient intensity, the light source of interferometric filter ELIASA is typically designed work(
Rate is all bigger, and supply voltage requires higher, and this is just according into interferometric filter ELIASA volume is big, quality is big, floor space
Greatly, power consumption is big.The species that these features are all only applicable to laboratory inspection sample simply tests use, is not suitable for using several samples
Mixing is examined or same sample many kinds of substance examines composite test simultaneously, can not meet mobile inspection laboratory or sampling observation sample
The demand of field test.
Generally, ELIASA and spectrophotometer are two kinds of instruments, want to realize enzyme linked immunosorbent assay and AAS point
Not Ce Liang testing liquid just need buy two instruments, add buying expenses and floor space of instrument and equipment etc..Actually
ELIASA and spectrophotometer are all colorimeters, and both can use same set of light-source system, realize two kinds of methods of inspection.Interference
Filter type ELIASA is only capable of using as the test of the simple sample in laboratory, can not be used as hybrid test or composite test
Test uses, can not large-scale popularization use.
By the retrieval of patent document, related patent document is not found.Therefore the present patent application has wound
The property made and novelty.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of full spectrum for being designed with eight passage cell holders
ELIASA, solve the problems, such as be：ELIASA inspection wavelength is limited, causes to examine the restricted problem of project kind；ELIASA is examined
Survey process operation it is cumbersome, by man's activity it is bigger the problem of；ELIASA can not realize the problem of AAS is examined；Enzyme
Mark instrument can not quickly realize full spectral scan problem and can not realize in a kind of liquid to be checked while examine a variety of materials to be checked
The problem of concentration.
The purpose of the present invention takes following technical scheme to realize：
A kind of full spectrum ELIASA for being designed with eight passage cell holders, including tungsten lamp, concave mirror, monochrometer, optical fiber
Beam, fiber bench, ELISA Plate and photoelectric sensor, the all-wave length light that tungsten lamp is sent enter single after concave mirror deflection angle
Color meter, the monochromatic light of desired wavelength can be adjusted out rapidly in monochrometer, the monochromatic light is ten uniform by fibre bundle
Light splitting is fine, wherein eight are main fiber, one is that spectrophotometric optical fiber is used to connect spectrophotometric module, and another is monitoring light
Fibre is used to connect monitoring optical fiber head；Eight main fiber uniform intervals are fixed in fiber bench, and fiber bench carries out optically focused to monochromatic light
Incident after processing to enter in the ELISA Plate equipped with solution to be measured, the light of some is remained by the liquid absorption to be checked in ELISA Plate
The monochromatic light of remaining part point projects ELISA Plate, and last light, which incides, is changed into electric signal in the photoelectric sensor of ELIASA, leads to
Cross the power of the photoelectric sensor electric signal of analysis ELIASA.
Moreover, the spectrophotometric optical fiber is connected on the optical fiber head of spectrophotometric module, the spectrophotometric module includes
Cuvette pallet, optical fiber head and rotary shaft, optical fiber headstock, cuvette pallet are disc, are uniformly spaced are provided with footpath thereon
Cuvette, the cuvette are eight；Rotary shaft is fixedly mounted with the axis position of cuvette pallet bottom, in the bottom of cuvette pallet
Lower section side is coaxially fixedly mounted with optical fiber headstock, and the optical fiber headstock is formed with groove in the cuvette that corresponding cuvette pallet is installed, with
Realize that cuvette can pass through from the groove internal clearance of optical fiber headstock when cuvette pallet rotates；It is horizontal solid on the outside of optical fiber headstock
Optical fiber head is filled, the made recess edge of optical fiber headstock in front of the optical fiber head is coaxially fixedly mounted with a front end collector lens, to should
Another lateral edges of optical fiber headstock groove of front end collector lens are coaxially fixedly mounted with a rear end collector lens, the rear end collector lens connection
One full spectrum light electric transducer.
Moreover, the monitoring optical fiber is connected on monitoring optical fiber head, the incident light direct irradiation that the monitoring light bore bit comes out
In monitoring light path photoelectric sensor.
The advantages and positive effects of the present invention are：
1st, this ELIASA can be with the concentration of automatic detection substance solution to be checked, and dual wavelength checked operation can once be completed to treat
The inspection of solution is examined, reduces artificial factor interference, can accurately detect the concentration of the molten thing of material, can be arbitrarily from inspection wavelength
(wavelength 200nm~1100nm), full spectral scan can also be carried out to liquid to be checked, find out the suitable inspection by attributes of liquid to be checked
Wavelength, meet needs of the different detecting liquids to spectrum, it is possible to achieve at the same in test solution many kinds of substance content.
2nd, this ELIASA can realize the inspection of enzyme linked immunological hair and two kinds of methods of inspection of AAS on an instrument,
Can be with off-line operation, testing result can pass inspection center back in real time by GPRS technologies, storage analysis, upload and print detection
Data, detection real-time data sharing can be realized with inspection center.
3rd, this ELIASA can accurately detect the concentration value of solution soluble thing to be measured, there is repeatability and the degree of accuracy well,
Reference instrument can be used as to configure the detection unit and research institution etc. such as each hospital, health and epidemic prevention station；All-wave length can be realized
Material to be checked is examined, meets the needs that many kinds of substance is examined；The instrument that instrument buying expenses and saving laboratory can be saved accounts for
Ground area.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the vertical view enlarged diagram (broken section) of spectrophotometric module of the present invention；
Fig. 3 is Fig. 2 A-A to cross-sectional view.
Embodiment
Embodiments of the invention are further described below in conjunction with accompanying drawing；The present embodiment is descriptive, is not to limit
Property, it is impossible to thus limit protection scope of the present invention.
A kind of full spectrum ELIASA for being designed with eight passage cell holders, including tungsten lamp 7, concave mirror 6, monochrometer 8, light
Fine beam, fiber bench 5, ELISA Plate 4 and photoelectric sensor 3, the all-wave length light that tungsten lamp is sent is after concave mirror deflection angle
Into monochrometer, the monochromatic light (wavelength 200nm-1100nm) of desired wavelength, the monochrome can be adjusted out rapidly in monochrometer
Light is that ten uniform light splitting are fine by fibre bundle, wherein eight are main fiber 11, one is spectrophotometric optical fiber 9, Ling Yigen
To monitor optical fiber 12；Eight main fiber uniform intervals are fixed in fiber bench, after fiber bench carries out the processing such as optically focused to monochromatic light
Incidence enters in the ELISA Plate equipped with solution to be measured, and then the light of some is by the liquid absorption to be checked in ELISA Plate, residue
Partial monochromatic light projects ELISA Plate, and last light, which incides, is changed into electric signal in the photoelectric sensor of ELIASA, pass through
Analyze the power of the photoelectric sensor electric signal of ELIASA, it is possible to the respective concentration of liquid solution to be checked is calculated.In enzyme
In the case that target liquid to be checked is constant, different wavelength is adjusted by monochrometer liquid to be checked is continuously measured, this is just
The spectral scan to solution to be checked is realized, solution to be checked has been carried out once absorbing numerical value using the monochromatic light of each wavelength
Front and rear contrast, the characteristic wavelength of liquid to be checked can be found out and realize in same liquid to be checked while examine many kinds of substance
Concentration purpose.
Spectrophotometric optical fiber during ten light splitting are fine is connected on the optical fiber head 20 of spectrophotometric module 10, the spectrophotometric
The structure of module is made up of, the cuvette support referring to Fig. 2,3 cuvette pallet 13, optical fiber head and rotary shaft 21, optical fiber headstock 19
Disk is disc, is uniformly spaced with footpath is provided with cuvette 14 thereon, and the cuvette is eight, and the quantity can also be according to reality
Need to be set；Rotary shaft is fixedly mounted with by fastening bolt 15 at the axis position of cuvette pallet bottom, the rotary shaft is by outer
Portion's power drive, which is realized, to be rotated, and thus cuvette pallet, which is realized, rotates.Side is coaxially fixedly mounted with below the bottom of cuvette pallet
Optical fiber headstock, the optical fiber headstock are formed with groove in the cuvette that corresponding cuvette pallet is installed, to realize that cuvette pallet turns
Cuvette can pass through from the groove internal clearance of optical fiber headstock when dynamic；Level is fixedly mounted with optical fiber head on the outside of optical fiber headstock, in the light
The made recess edge of optical fiber headstock in front of fine head is coaxially fixedly mounted with a front end collector lens 18, to should front end collector lens
Another lateral edges of optical fiber headstock groove are coaxially fixedly mounted with a rear end collector lens 17, and the rear end collector lens connects a full spectrum light fax
Sensor 16.
The operation principle of spectrophotometric module is：
Optical fiber head is fixed on the optical fiber headstock of spectrophotometric module, and the monochromatic light that optical fiber head is sent is saturating by front end optically focused
Mirror is changed into a branch of directional light.Eight cuvettes are uniformly installed on cuvette pallet, cuvette pallet install on the rotary shaft, than
Color ware pallet can gradually pass through test position with eight cuvettes in the horizontal plane.The optical fiber head of spectrophotometric module was penetrated
In the incident cuvette equipped with solution to be measured of parallel rays come, a part of incident light is absorbed by the solution to be measured in cuvette,
Remaining a part of beam projecting cuvette, then incide the photoelectric transfer composed entirely by rear end collector lens optically focused, last light
In sensor.The change that optical signals can be measured in the photoelectric sensor composed entirely causes the variable quantity size of electric signal, so that it may
To measure the concentration of the solution to be measured in cuvette.In the case of constant when cuvette position, adjusted by monochrometer different
Wavelength is continuously measured liquid to be checked, and which achieves the spectral scan to solution to be checked, solution to be checked is used each
The monochromatic light of wavelength has carried out once absorbing the front and rear contrast of numerical value, can find out the characteristic wavelength of liquid to be checked and realize
Many kinds of substance concentration purpose is examined in same liquid to be checked simultaneously.
Monitoring optical fiber during ten light splitting are fine is connected on monitoring optical fiber head 2, and the incident light that the monitoring light bore bit comes out is straight
Connect and be radiated in monitoring light path photoelectric sensor 1.The purpose of design of the light path is to monitor monochromatic intensity in monochrometer
Change, the change of the intensity of light corrects enzyme mark module and spectrophotometric module detection data, the inspection knot so obtained in real time
Fruit is more accurate.
This invention simplifies the operating process of instrument, has been also convenient for operator's use, can be complete in real time in an instrument
The enzyme linked immunological hair of wavelength is examined and two kinds of methods of inspection of AAS.
The present invention need to only load onto liquid to be checked in ELISA Plate, press and examine key to examine automatically, it is possible to achieve once grasp
Work can complete dual wavelength inspection, and can goes out result within 10 seconds.
The present invention has temperature to check function, can correct inspection data automatically according to the temperature at inspection scene.
The present invention can store 100,000 inspection datas, can carry out data analysis to these data, these can be counted
Counted according to uploading
Calculation machine or printer, testing result can pass inspection center back in real time by GPRS technologies.
Claims (3)
- A kind of 1. full spectrum ELIASA for being designed with eight passage cell holders, it is characterised in that：Including tungsten lamp, concave mirror, list Color meter, fibre bundle, fiber bench, ELISA Plate and photoelectric sensor, the all-wave length light that tungsten lamp is sent pass through concave mirror deflection angle Enter monochrometer after degree, the monochromatic light of desired wavelength can be adjusted out rapidly in monochrometer, the monochromatic light is by fibre bundle Ten uniform light splitting are fine, wherein eight are main fiber, one is that spectrophotometric optical fiber is used to connect spectrophotometric module, another Root is used to connect monitoring optical fiber head for monitoring optical fiber；Eight main fiber uniform intervals are fixed in fiber bench, and fiber bench is to monochrome Incidence enters in the ELISA Plate equipped with solution to be measured after light carries out optically focused processing, and the light of some is by the liquid to be checked in ELISA Plate Body absorbs, and the monochromatic light of remainder projects ELISA Plate, and last light, which is incided in the photoelectric sensor of ELIASA, to be changed into Electric signal, the power of the photoelectric sensor electric signal by analyzing ELIASA.
- 2. the full spectrum ELIASA according to claim 1 for being designed with eight passage cell holders, it is characterised in that：The light splitting Luminosity optical fiber is connected on the optical fiber head of spectrophotometric module, and the spectrophotometric module includes cuvette pallet, optical fiber head and rotation Rotating shaft, optical fiber headstock, cuvette pallet are disc, are uniformly spaced with footpath are provided with cuvette thereon, and the cuvette is eight； Rotary shaft is fixedly mounted with the axis position of cuvette pallet bottom, side is coaxially fixedly mounted with optical fiber head below the bottom of cuvette pallet Seat, the optical fiber headstock are formed with groove in the cuvette that corresponding cuvette pallet is installed, to realize that cuvette pallet rotates when ratio Color ware can pass through from the groove internal clearance of optical fiber headstock；Level is fixedly mounted with optical fiber head on the outside of optical fiber headstock, before the optical fiber head Side the made recess edge of optical fiber headstock be coaxially fixedly mounted with a front end collector lens, to should front end collector lens optical fiber headstock Another lateral edges of groove are coaxially fixedly mounted with a rear end collector lens, and the rear end collector lens connects a full spectrum light electric transducer.
- 3. the full spectrum ELIASA according to claim 1 for being designed with eight passage cell holders, it is characterised in that：The monitoring Optical fiber is connected on monitoring optical fiber head, and the incident light direct irradiation that the monitoring light bore bit comes out is in monitoring light path photoelectric sensor It is interior.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710853786.5A CN107561073A (en) | 2017-09-20 | 2017-09-20 | A kind of full spectrum ELIASA for being designed with eight passage cell holders |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710853786.5A CN107561073A (en) | 2017-09-20 | 2017-09-20 | A kind of full spectrum ELIASA for being designed with eight passage cell holders |
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| CN107561073A true CN107561073A (en) | 2018-01-09 |
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| CN201710853786.5A Withdrawn CN107561073A (en) | 2017-09-20 | 2017-09-20 | A kind of full spectrum ELIASA for being designed with eight passage cell holders |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110411963A (en) * | 2019-08-08 | 2019-11-05 | 重庆科技学院 | A kind of portable spectrophotometer of Heavy Metals in Waters ion detection |
| CN112014343A (en) * | 2020-09-27 | 2020-12-01 | 苏州卡睿知光电科技有限公司 | Spectrophotometry detection system with all-in-one light source |
| CN113358650A (en) * | 2021-06-01 | 2021-09-07 | 浙江大学 | 96-hole microporous plate reader |
| CN113552337A (en) * | 2021-07-19 | 2021-10-26 | 中山大学 | Full-spectrum high-efficiency microplate reader system |
| CN117074344A (en) * | 2023-08-24 | 2023-11-17 | 上海暄泓科学仪器有限公司 | Handheld ultraviolet spectrometer |
| CN117368145A (en) * | 2023-11-17 | 2024-01-09 | 无锡迅杰光远科技有限公司 | Near infrared spectrum detection system and detection method for chemical liquid |
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| CN201754145U (en) * | 2010-08-09 | 2011-03-02 | 潍坊瑞格测试仪器有限公司 | Optical detecting device of residual toxicity rapid testing meter |
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| CN117074344B (en) * | 2023-08-24 | 2024-02-20 | 上海暄泓科学仪器有限公司 | Handheld ultraviolet spectrometer |
| CN117368145A (en) * | 2023-11-17 | 2024-01-09 | 无锡迅杰光远科技有限公司 | Near infrared spectrum detection system and detection method for chemical liquid |
| CN117368145B (en) * | 2023-11-17 | 2024-06-07 | 无锡迅杰光远科技有限公司 | Near infrared spectrum detection system and detection method for chemical liquid |
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Application publication date: 20180109 |
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