CN112014343A - Spectrophotometry detection system with all-in-one light source - Google Patents

Spectrophotometry detection system with all-in-one light source Download PDF

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Publication number
CN112014343A
CN112014343A CN202011035407.XA CN202011035407A CN112014343A CN 112014343 A CN112014343 A CN 112014343A CN 202011035407 A CN202011035407 A CN 202011035407A CN 112014343 A CN112014343 A CN 112014343A
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China
Prior art keywords
module
light
detection
sample
light source
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Pending
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CN202011035407.XA
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Chinese (zh)
Inventor
夏明亮
袁邵隆
张峰
高响
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SHANGHAI SUPORE INSTRUMENTS Co.,Ltd.
SUZHOU KARUIZHI PHOTOELECTRIC TECHNOLOGY Co.,Ltd.
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Suzhou Karuizhi Photoelectric Technology Co ltd
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Priority to CN202011035407.XA priority Critical patent/CN112014343A/en
Publication of CN112014343A publication Critical patent/CN112014343A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38Diluting, dispersing or mixing samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106General arrangement of respective parts
    • G01N2021/0112Apparatus in one mechanical, optical or electronic block
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N2021/3129Determining multicomponents by multiwavelength light

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The invention discloses a spectrophotometric detection system with multiple integrated light sources, which comprises: the device comprises an all-in-one light source module, a light guide module, a sample disc module and a photoelectric detection module; the light source module that unifies more waits to detect the sample according to the difference, the output corresponds the detection light of wavelength and intensity to leaded light module, carry out homogenization treatment at leaded light module, the even detection light of output, even detection light shines to waiting of sample dish module and detects sample department through the inspection hole of sample dish module, photoelectric detection module receives the detection light of waiting to detect the sample through the inspection hole, carry out spectrophotometric detection to the sample that awaits measuring. The invention uniformly mixes all-in-one detection light through the light guide module by the light guide module, irradiates the detection light into a sample to be detected in the sample disc, and transmits the detection light to the photoelectric detection module for signal detection.

Description

Spectrophotometry detection system with all-in-one light source
Technical Field
The invention relates to the technical field of instrument detection, in particular to a spectrophotometry detection system with multiple integrated light sources.
Background
The core of the spectrophotometer is to measure the transmittance and reflectance of light of a substance at a specific wavelength or within a certain wavelength range, the spectrophotometer is widely applied to modern industrial production and management departments such as physics, chemistry, biology, medicine, materials science, environmental science, chemical industry, medicine, environmental detection, metallurgy and the like, a light path is formed by utilizing the tension traction of a trace amount of liquid, qualitative or quantitative analysis is carried out on the substance through the absorbance of the light at the specific wavelength or within the certain wavelength range, and accurate detection data can be obtained only by a trace amount of sample to be detected.
At present, one commonly used spectrophotometer is to realize the absorption rate measurement of high spectral resolution in a wide spectral range by using a white light source for illumination and splitting light through a prism or a grating, and has the advantages of wide application range, high precision, high price, large volume, relatively complex operation, need of professional technicians and inapplicability to the rapid detection requirement under the field condition. The other method is to adopt a multicolor selectable optical filter to carry out multiband absorbance determination, the optical filter mode is divided into a static mode and a dynamic mode, in the static mode, each optical filter corresponds to one detection channel, each channel is provided with a respective photoelectric processing unit, a plurality of channels are simultaneously measured during measurement, although the speed is high, a single channel only has a single fixed wavelength, the channel utilization rate is low, the cost is quite high, and the volume is large; in a dynamic mode, a common optical filter wheel type structure detects by rotating a color filter to a specific wavelength, the mode shares one path of photoelectric acquisition and processing system, the cost is relatively low, but the requirement on the angular precision of the color filter is very high, the control is relatively complex, and the instrument stability is poor. The problems of high price, complex structure, complex test flow and inaccurate data exist when the spectrophotometry detection is carried out.
Disclosure of Invention
Therefore, the spectrophotometric detection system with the all-in-one light source provided by the invention overcomes the defects of high price, complex structure, large volume, complicated test flow and inaccurate data in spectrophotometric detection.
In order to achieve the purpose, the invention provides the following technical scheme:
the embodiment of the invention provides a spectrophotometric detection system with an all-in-one light source, which comprises: the device comprises an all-in-one light source module, a light guide module, a sample disc module and a photoelectric detection module;
the all-in-one light source module is used for outputting detection light with corresponding wavelength and intensity according to different samples to be detected;
the light guide module is used for carrying out homogenization treatment on the detection light and outputting uniform detection light;
the sample disc module is used for bearing a sample to be detected and irradiating uniform detection light to the sample to be detected through the detection hole of the sample disc module;
and the photoelectric detection module is used for receiving the detection light of the sample to be detected passing through the detection hole and performing spectrophotometric detection on the sample to be detected.
In one embodiment, the all-in-one light source module includes: the LED light source and the first control module;
the LED light source is used for emitting light sources with different wavelengths and different intensities;
and the first control module is used for triggering the light output by the LED light source according to the wavelength and the intensity of the light source required by the sample to be detected to obtain the detection light corresponding to the sample to be detected.
In one embodiment, the light guide module includes: the device comprises a fixing device, a light homogenizing module and a light guide optical fiber;
the fixing device is used for fixedly connecting the all-in-one light source module with the light homogenizing module;
the light homogenizing module is used for homogenizing the detection light;
and the light guide optical fiber diameter is used for outputting the detection light after the homogenization treatment to a sample to be detected.
In one embodiment, the diameter of the light guide fiber is not smaller than the diameter of the detection light.
In one embodiment, the sample tray module comprises: the sample tray, the rotating motor and the second control module;
the sample tray is used for placing cuvettes for containing samples to be detected, a plurality of detection positions are arranged on the sample tray, and each detection position is correspondingly provided with one cuvette;
the second control module is used for sending a control command and controlling the rotating motor;
and the rotating motor is used for rotating the sample tray to enable the inspection light after the homogenization treatment to shine into the detection hole of the sample tray module through the cuvette.
In one embodiment, the photodetection module comprises: photoelectric detection circuit board, photoelectric detection sensor; the photoelectric detection sensor is welded on the photoelectric detection circuit board, and the detection surface of the photoelectric detection sensor, the detection hole of the sample disk module and the output end of the light guide optical fiber are on the same horizontal line.
The invention provides a spectrophotometric detection system of an all-in-one light source, which comprises: the device comprises an all-in-one light source module, a light guide module, a sample disc module and a photoelectric detection module; the light source module that unifies more waits to detect the sample according to the difference, the output corresponds the detection light of wavelength and intensity to leaded light module, carry out homogenization treatment at leaded light module, the even detection light of output, even detection light shines to waiting of sample dish module and detects sample department through the inspection hole of sample dish module, photoelectric detection module receives the detection light of waiting to detect the sample through the inspection hole, carry out spectrophotometric detection to the sample that awaits measuring. The invention uniformly mixes all-in-one detection light through the light guide module by the light guide module, irradiates the detection light into a sample to be detected in the sample disc, and transmits the detection light to the photoelectric detection module for signal detection.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic diagram of a specific example of a spectrophotometric detection system with an all-in-one light source according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a sample tray module of a spectrophotometric detection system with an integrated light source according to an embodiment of the present invention;
fig. 3 is a cross-sectional view of an embodiment of a spectrophotometric detection system with an all-in-one light source according to an embodiment of the invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Examples
The present embodiment provides a spectrophotometric detecting system with an all-in-one light source, as shown in fig. 1, including: the device comprises a light source module, a light guide module, a sample disc module and a photoelectric detection module.
In the embodiment of the invention, the all-in-one light source module is used for outputting detection light with corresponding wavelength and intensity according to different samples to be detected; wherein, unify light source module all the time includes: the LED light source and the first control module; the LED light source is used for emitting light sources with different wavelengths and different intensities; and the first control module is used for triggering the light output by the LED light source according to the wavelength and the intensity of the light source required by the sample to be detected to obtain the detection light corresponding to the sample to be detected.
In the embodiment of the invention, the light guide module is used for carrying out homogenization treatment on the detection light and outputting uniform detection light; wherein, light guide module includes: the device comprises a fixing device, a light homogenizing module and a light guide optical fiber; the fixing device is used for fixedly connecting the all-in-one light source module with the light homogenizing module; the light homogenizing module is used for homogenizing detection light, usually uses a light homogenizing sheet for processing, and correspondingly changes the light homogenizing sheet according to actual requirements in actual application; and the diameter of the light guide optical fiber is not smaller than that of the detection light, and the light guide optical fiber is used for outputting the detection light after the homogenization treatment to a sample to be detected. Make the intensity and the degree of consistency that the detection light that corresponds to treat the sample shines into the detection sample through leaded light module, guarantee that the signal detection precision is higher, and data stability is better.
In the embodiment of the present invention, as shown in fig. 2, the sample tray module is configured to carry a sample to be detected, and irradiate uniform detection light to the sample to be detected through the detection hole of the sample tray module; wherein the sample tray module comprises: the device comprises a sample tray 1, a rotating motor 2 and a second control module; the device comprises a sample tray 1, a plurality of detection positions 3 and a detection hole, wherein the sample tray 1 is used for placing cuvettes for containing samples to be detected, each detection position 3 is correspondingly placed with one cuvette for containing samples to be detected, and the detection hole penetrates through the corresponding detection position to form a detection channel for penetrating detection light to the samples to be detected; the second control module is used for sending a control command and controlling the rotating motor; and the rotating motor 2 is used for rotating the sample tray 1 to enable the homogenized inspection light to shine into the detection hole 4 of the sample tray module through the cuvette. Through the rotatory mode of rotating electrical machines 2, drive sample tray 1 and rotate and detect to required detection channel, simultaneously accessible control rotating electrical machines 2 rotates scram again, drives and waits to examine the sample liquid mixing.
It should be noted that the first control module and the second control module are controlled by using a single chip microcomputer in the prior art, wherein when the first control module is used for controlling, according to detection light required by selecting a sample to be detected, the single chip microcomputer sends a control instruction to trigger a corresponding wavelength to the first control module, and after receiving the control instruction, the first control module lights up LEDs with the corresponding wavelength.
In the embodiment of the invention, the photoelectric detection module is used for receiving the detection light of the sample to be detected passing through the detection hole and carrying out spectrophotometric detection on the sample to be detected. Wherein, the photodetection module includes: photoelectric detection circuit board, photoelectric detection sensor; the photoelectric detection sensor is welded on the photoelectric detection circuit board, and the detection surface of the photoelectric detection sensor, the detection hole of the sample disk module and the output end of the light guide optical fiber are on the same horizontal line.
In the embodiment of the invention, all channel detection can be finished by only 1-path detection device (consisting of 1 all-in-one light source module and 1 photoelectric detection module), so that the system cost and space are effectively reduced, the cost advantage is higher when the number of channels is larger, and the difference between multiple light sources and multiple detectors does not exist, so that the detection deviation among the channels is smaller, and the detection stability and precision are higher.
In this embodiment, the spectrophotometric detecting system with the all-in-one light source shown in fig. 3 works as follows:
a plurality of samples to be detected are placed in a cuvette, the cuvette is placed at the detection position of a sample tray, the samples to be detected are detected according to different through an all-in-one light source module 5, the detection light with corresponding wavelength and intensity is output, the all-in-one light source module 5 is fixedly connected with a light homogenizing module 7 through a fixing device 6, the detection light is homogenized through the light homogenizing module 7, the diameter of a light guide optical fiber 8 is not smaller than that of the detection light, the detection light after homogenization treatment is transmitted to the samples to be detected 9, the detection light after homogenization treatment is irradiated to the samples to be detected of the sample tray module through a detection hole of the sample tray module by controlling a rotating motor, the detection light of the samples to be detected through the detection hole is received at a photoelectric detection module 10, and the detection of spectrophotometry is performed on the samples to be detected, wherein the detection surface of a photoelectric detection sensor, The output ends of the detection hole and the light guide optical fiber of the sample disc module are on the same horizontal line, and after the photoelectric detector obtains data, other channels are repeatedly detected through the rotating motor, and spectrophotometry detection is carried out on a sample to be detected.
In the embodiment of the invention, all channel detection can be finished by only one detection device (consisting of one all-in-one light source module 1 and one photoelectric detection module 10), so that the system cost and space are effectively reduced, and the more channels, the greater the cost advantage and the absence of difference between multiple light sources and multiple detectors, so that the detection deviation among channels is smaller, the detection stability and precision are higher, the cost is low, the structure is simple, and the test flow is simple and easy to realize.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.

Claims (6)

1. A spectrophotometric detecting system with all-in-one light source is characterized by comprising: the device comprises an all-in-one light source module, a light guide module, a sample disc module and a photoelectric detection module;
the all-in-one light source module is used for outputting detection light with corresponding wavelength and intensity according to different samples to be detected;
the light guide module is used for carrying out homogenization treatment on the detection light and outputting uniform detection light;
the sample disc module is used for bearing a sample to be detected and irradiating uniform detection light to the sample to be detected through the detection hole of the sample disc module;
and the photoelectric detection module is used for receiving the detection light of the sample to be detected passing through the detection hole and performing spectrophotometric detection on the sample to be detected.
2. The spectrophotometric detecting system of claim 1 wherein the all-in-one light source module comprises: the LED light source and the first control module;
the LED light source is used for emitting light sources with different wavelengths and different intensities;
and the first control module is used for triggering the light output by the LED light source according to the wavelength and the intensity of the light source required by the sample to be detected to obtain the detection light corresponding to the sample to be detected.
3. The spectrophotometric detecting system of claim 1 or 2 wherein the light guide module comprises: the device comprises a fixing device, a light homogenizing module and a light guide optical fiber;
the fixing device is used for fixedly connecting the all-in-one light source module with the light homogenizing module;
the light homogenizing module is used for homogenizing the detection light;
and the light guide optical fiber is used for outputting the detection light after the homogenization treatment to a sample to be detected.
4. The spectrophotometric detecting system of claim 3 wherein the diameter of the light guiding fiber is not smaller than the diameter of the detecting light.
5. The spectrophotometric detecting system of an all-in-one light source as claimed in claim 1, wherein the sample tray module comprises: the sample tray, the rotating motor and the second control module;
the sample tray is used for placing cuvettes for containing samples to be detected, a plurality of detection positions are arranged on the sample tray, and each detection position is correspondingly provided with one cuvette;
the second control module is used for sending a control command and controlling the rotating motor;
and the rotating motor is used for rotating the sample tray to enable the inspection light after the homogenization treatment to shine into the detection hole of the sample tray module through the cuvette.
6. The spectrophotometric detecting system of claim 3 wherein the photodetection module comprises: photoelectric detection circuit board, photoelectric detection sensor; the photoelectric detection sensor is welded on the photoelectric detection circuit board, and the detection surface of the photoelectric detection sensor, the detection hole of the sample disk module and the output end of the light guide optical fiber are on the same horizontal line.
CN202011035407.XA 2020-09-27 2020-09-27 Spectrophotometry detection system with all-in-one light source Pending CN112014343A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011035407.XA CN112014343A (en) 2020-09-27 2020-09-27 Spectrophotometry detection system with all-in-one light source

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011035407.XA CN112014343A (en) 2020-09-27 2020-09-27 Spectrophotometry detection system with all-in-one light source

Publications (1)

Publication Number Publication Date
CN112014343A true CN112014343A (en) 2020-12-01

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Country Status (1)

Country Link
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Effective date of registration: 20210316

Address after: 215000 room 504-1, Arts crafts emporium, 689 Binhe Road, hi tech Zone, Suzhou, Jiangsu.

Applicant after: SUZHOU KARUIZHI PHOTOELECTRIC TECHNOLOGY Co.,Ltd.

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Address before: 215000 room 504-1, Arts crafts emporium, 689 Binhe Road, hi tech Zone, Suzhou, Jiangsu.

Applicant before: SUZHOU KARUIZHI PHOTOELECTRIC TECHNOLOGY Co.,Ltd.