CN207366450U - A kind of full spectrum microplate reader for being designed with eight passage cell holders - Google Patents
A kind of full spectrum microplate reader for being designed with eight passage cell holders Download PDFInfo
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- CN207366450U CN207366450U CN201721207878.8U CN201721207878U CN207366450U CN 207366450 U CN207366450 U CN 207366450U CN 201721207878 U CN201721207878 U CN 201721207878U CN 207366450 U CN207366450 U CN 207366450U
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Abstract
It the utility model is related to a kind of full spectrum microplate reader for being designed with eight passage cell holders, including tungsten lamp, concave mirror, monochrometer, fiber optic bundle, fiber bench, ELISA Plate and photoelectric sensor, the all-wave length light that tungsten lamp is sent enters monochrometer after concave mirror deflection angle, the monochromatic light of desired wavelength can be adjusted out rapidly in monochrometer, the monochromatic light is fine for ten uniform light splitting by fiber optic bundle, wherein eight are main fiber, one is used to connect spectrophotometric module for spectrophotometric optical fiber, and another is used to connect monitoring optical fiber head for monitoring optical fiber.This microplate reader can accurately detect the concentration value of solution soluble thing to be measured, there is repeatability and accuracy well, and reference instrument can be used as to configure the detection unit and research institution etc. such as each hospital, health and epidemic prevention station；It can realize that all-wave length examines material to be checked, meet the needs that many kinds of substance is examined；Instrument buying expenses can be saved and save the instrument floor space in laboratory.
Description
Technical field
The utility model belongs to medical test or chemical analysis field, and being related to microplate reader, especially one kind, to be designed with eight logical
The full spectrum microplate reader of road cell holder.
Background technology
Microplate reader in field of medical examination at present, is substantially based on light of the interferometric filter as monochromatic light discrete device
Electric colorimetric instrument.The testing principle of interference filter chip microplate reader is：The light that tungsten lamp is sent is irradiated into interferometric filter selection
Disk, disk is selected by rotational interference optical filter, can rotate to the interferometric filter of fixed wave length, and tungsten lamp incident light is dry by this
Relate to the monochromatic light that fixed wave length has just been waited until after optical filter.Monochromatic light passes through optical fibre light splitting, forms eight energy uniformly small light
Fibre, eight small optical fiber are fixed on fiber fixed seat, and the uniform light of eight beams is projected from the lens on fiber fixed seat, is transmitted through
ELISA Plate, some light are gone out the liquid in ELISA Plate, are radiated at by the liquid absorption to be checked in ELISA Plate, remaining light transmission
In photoelectric sensor, optical signal is changed into electric signal in photoelectric sensor.The electric signal obtained by analyzing photoelectric sensor
It is strong and weak, it is possible to which that analysis draws the strength of fluid in ELISA Plate.Its there are the problem of it is as follows：1st, interference filter chip enzyme is being used
, it is necessary to first recall main inspection wavelength when mark instrument measurement testing concentration, put on ELISA Plate plus artificial zero check, Ran Houjian
Solution to be checked is tested, then recalls secondary inspection wavelength, ELISA Plate will also be put plus artificial zero check, examine solution to be checked again, a kind of
The inspection of solution needs multi-pass operation, easily because the error of operating personnel causes the deviation of testing result；2nd, interferometric filter enzyme
The optical filter for marking instrument sets limited amount, and each interferometric filter wavelength is fixed, and general instrument is not over 8, main inspection ripple
The long and secondary selection generally each 4 for examining wavelength, it is impossible to meet the inspection needs of a variety of kind of inspection；3rd, interferometric filter enzyme mark
It is usually 8~10nm that instrument half-wavelength is very wide, and monochromatic wavelength variation range is big, and wavelength is inaccurate, the inspection accuracy of instrument
Difference；4th, interference filter on piece is coated with many layer films, and the film of these multilayers prevents the biography of monochromatic light energy to a certain extent
Broadcast；The monochromatic light of interferometric filter microplate reader sufficient intensity in order to obtain, the light source of interferometric filter microplate reader are typically designed work(
Rate is all bigger, and supply voltage requirement is higher, this is just according into interferometric filter microplate reader volume is big, quality is big, floor space
Greatly, energy consumption is big.The species that these features are all only applicable to laboratory inspection sample simply tests use, is not suitable for using several samples
Mixing is examined or same sample many kinds of substance examines composite test at the same time, can not meet mobile inspection laboratory or sampling observation sample
The demand of field test.
Under normal circumstances, microplate reader and spectrophotometer are two kinds of instruments, to realize enzyme linked immunosorbent assay and spectrophotometry point
Not Ce Liang testing liquid just need buy two instruments, add buying expenses and floor space of instrument and equipment etc..Actually
Microplate reader and spectrophotometer are all colorimeters, both can use same set of light-source system, realize two kinds of methods of inspection.Interference
Filter type microplate reader is only capable of using as the test of the simple sample in laboratory, can not be used as hybrid test or composite test
Test uses, can not large-scale popularization use.
By the retrieval of patent document, related patent document is not found.Therefore present utility model application tool
Creative and novelty.
Utility model content
The purpose of the utility model is to overcome the deficiencies in the prior art, there is provided a kind of eight passage cell holders of being designed with
Full spectrum microplate reader, solve the problems, such as be：Microplate reader inspection wavelength is limited, causes to examine the restricted problem of project kind；Enzyme mark
Instrument detection process is cumbersome, the problem of being subject to man's activity bigger；Microplate reader can not achieve asking for spectrophotometry inspection
Topic；Microplate reader can not quickly realize full spectral scan problem and can not realize in a kind of liquid to be checked while examine a variety of to be checked
The problem of material concentration.
The purpose of this utility model takes following technical scheme to realize：
A kind of full spectrum microplate reader for being designed with eight passage cell holders, including tungsten lamp, concave mirror, monochrometer, optical fiber
Beam, fiber bench, ELISA Plate and photoelectric sensor, the all-wave length light that tungsten lamp is sent enter single after concave mirror deflection angle
Color meter, can adjust out the monochromatic light of desired wavelength rapidly in monochrometer, and the monochromatic light is uniform for ten by fiber optic bundle
Light splitting is fine, wherein eight are main fiber, one is used to connect spectrophotometric module for spectrophotometric optical fiber, and another is monitoring light
Fibre is used to connect monitoring optical fiber head；Eight main fiber uniform intervals are fixed in fiber bench, and fiber bench carries out optically focused to monochromatic light
Incident after processing to enter in the ELISA Plate equipped with solution to be measured, the light of some is remained by the liquid absorption to be checked in ELISA Plate
The monochromatic light of remaining part point projects ELISA Plate, and last light, which incides, is changed into electric signal in the photoelectric sensor of microplate reader, leads to
Cross the power of the photoelectric sensor electric signal of analysis microplate reader.
Moreover, the spectrophotometric optical fiber is connected on the optical fiber head of spectrophotometric module, which includes
Cuvette pallet, optical fiber head and rotation axis, optical fiber headstock, cuvette pallet are disc, are uniformly spaced are provided with footpath thereon
Cuvette, the cuvette are eight；Rotation axis is fixedly mounted with the axis position of cuvette pallet bottom, in the bottom of cuvette pallet
Lower section side is coaxially fixedly mounted with optical fiber headstock, which is formed with groove in the cuvette that corresponding cuvette pallet is installed, with
Realize that cuvette can pass through from the groove internal clearance of optical fiber headstock when cuvette pallet rotates；It is horizontal solid on the outside of optical fiber headstock
Optical fiber head is filled, the made recess edge of optical fiber headstock in front of the optical fiber head is coaxially fixedly mounted with a front end collector lens, is somebody's turn to do corresponding
Another lateral edges of optical fiber headstock groove of front end collector lens are coaxially fixedly mounted with a rear end collector lens, the rear end collector lens connection
One full spectrum light electric transducer.
Moreover, the monitoring optical fiber is connected on monitoring optical fiber head, the incident light direct irradiation which comes out
In monitoring light path photoelectric sensor.
The advantages of the utility model and good effect are：
1st, this microplate reader can detect the concentration of substance solution to be checked automatically, and dual wavelength checked operation can once be completed to treat
The inspection of solution is examined, artificial factor interference is reduced, can accurately detect the concentration of the molten thing of material, can arbitrarily select inspection wavelength
(wavelength 200nm~1100nm), can also carry out full spectral scan to liquid to be checked, find out the suitable inspection by attributes of liquid to be checked
Wavelength, meets needs of the different detecting liquids to spectrum, it is possible to achieve at the same in test solution many kinds of substance content.
2nd, this microplate reader can realize the inspection of enzyme linked immunological hair and two kinds of methods of inspection of spectrophotometry on an instrument,
Can be with off-line operation, testing result can pass inspection center back in real time by GPRS technologies, and storage analysis, upload and print detection
Data, can realize detection real-time data sharing with inspection center.
3rd, this microplate reader can accurately detect the concentration value of solution soluble thing to be measured, there is repeatability and accuracy well,
Reference instrument can be used as to configure the detection unit and research institution etc. such as each hospital, health and epidemic prevention station；It can realize all-wave length
Material to be checked is examined, meets the needs that many kinds of substance is examined；The instrument that instrument buying expenses and saving laboratory can be saved accounts for
Ground area.
Brief description of the drawings
Fig. 1 is the structure diagram of the utility model；
Fig. 2 is the vertical view enlarged diagram (broken section) of the utility model spectrophotometric module；
Fig. 3 is the A-A of Fig. 2 to cross-sectional view.
Embodiment
The embodiment of the utility model is further described below in conjunction with attached drawing；The present embodiment is descriptive, is not
Limited, it is impossible to thus limit the scope of protection of the utility model.
A kind of full spectrum microplate reader for being designed with eight passage cell holders, including tungsten lamp 7, concave mirror 6, monochrometer 8, light
Fine beam, fiber bench 5, ELISA Plate 4 and photoelectric sensor 3, the all-wave length light that tungsten lamp is sent is after concave mirror deflection angle
Into monochrometer, the monochromatic light (wavelength 200nm-1100nm) of desired wavelength, the monochrome can be adjusted out rapidly in monochrometer
Light is fine for ten uniform light splitting by fiber optic bundle, wherein eight are main fiber 11, one is spectrophotometric optical fiber 9, Ling Yigen
To monitor optical fiber 12；Eight main fiber uniform intervals are fixed in fiber bench, after fiber bench carries out monochromatic light the processing such as optically focused
Incidence enters in the ELISA Plate equipped with solution to be measured, and then the light of some is by the liquid absorption to be checked in ELISA Plate, residue
Partial monochromatic light projects ELISA Plate, and last light, which incides, is changed into electric signal in the photoelectric sensor of microplate reader, pass through
Analyze the power of the photoelectric sensor electric signal of microplate reader, it is possible to the respective concentration of liquid solution to be checked is calculated.In enzyme
In the case that target liquid to be checked is constant, different wavelength is adjusted by monochrometer liquid to be checked is continuously measured, this is just
The spectral scan to solution to be checked is realized, solution to be checked has been carried out once absorbing numerical value using the monochromatic light of each wavelength
Front and rear contrast, the characteristic wavelength of liquid to be checked can be found out and realize in same liquid to be checked while examine many kinds of substance
Concentration purpose.
Spectrophotometric optical fiber during ten light splitting are fine is connected on the optical fiber head 20 of spectrophotometric module 10, the spectrophotometric
The structure of module is made of, the cuvette support referring to Fig. 2,3 cuvette pallet 13, optical fiber head and rotation axis 21, optical fiber headstock 19
Disk is disc, is uniformly spaced with footpath is provided with cuvette 14 thereon, which is eight, which can also be according to reality
Need to be set；Rotation axis is fixedly mounted with by fastening bolt 15 at the axis position of cuvette pallet bottom, the rotation axis is by outer
Portion's power drive, which is realized, to be rotated, and thus cuvette pallet, which is realized, rotates.Side is coaxially fixedly mounted with below the bottom of cuvette pallet
Optical fiber headstock, the optical fiber headstock are formed with groove in the cuvette that corresponding cuvette pallet is installed, to realize that cuvette pallet turns
Cuvette can pass through from the groove internal clearance of optical fiber headstock when dynamic；Level is fixedly mounted with optical fiber head on the outside of optical fiber headstock, in the light
The made recess edge of optical fiber headstock in front of fine head is coaxially fixedly mounted with a front end collector lens 18, in the corresponding front end collector lens
Another lateral edges of optical fiber headstock groove are coaxially fixedly mounted with a rear end collector lens 17, which connects a full spectrum light fax
Sensor 16.
The operation principle of spectrophotometric module is：
Optical fiber head is fixed on the optical fiber headstock of spectrophotometric module, and the monochromatic light that optical fiber head is sent is saturating by front end optically focused
Mirror is changed into a branch of directional light.Eight cuvettes are uniformly installed on cuvette pallet, cuvette pallet install on the rotary shaft, than
Color ware pallet can gradually pass through test position with eight cuvettes in the horizontal plane.The optical fiber head of spectrophotometric module was penetrated
In the incident cuvette equipped with solution to be measured of parallel rays come, a part of incident light is absorbed by the solution to be measured in cuvette,
Remaining a part of beam projecting cuvette, then incide the photoelectric transfer composed entirely by rear end collector lens optically focused, last light
In sensor.The change that optical signals can be measured in the photoelectric sensor composed entirely causes the variable quantity size of electric signal, so that it may
To measure the concentration of the solution to be measured in cuvette.In the case of constant when cuvette position, adjusted by monochrometer different
Wavelength continuously measures liquid to be checked, and which achieves the spectral scan to solution to be checked, solution to be checked is used each
The monochromatic light of wavelength has carried out once absorbing the front and rear contrast of numerical value, can find out the characteristic wavelength of liquid to be checked and realize
Many kinds of substance concentration purpose is examined in same liquid to be checked at the same time.
Monitoring optical fiber during ten light splitting are fine is connected on monitoring optical fiber head 2, and the incident light which comes out is straight
Connect and be radiated in monitoring light path photoelectric sensor 1.The purpose of design of the light path is to monitor monochromatic intensity in monochrometer
Change, the change of the intensity of light corrects enzyme mark module and spectrophotometric module detection data, the inspection knot so obtained in real time
Fruit is more accurate.
The utility model simplifies the operating process of instrument, has been also convenient for operator's use, can be real in an instrument
When all-wave length enzyme linked immunological hair examine and two kinds of methods of inspection of spectrophotometry.
The utility model need to only load onto liquid to be checked in ELISA Plate, press and examine key to examine automatically, it is possible to achieve one
Secondary operation can complete dual wavelength inspection, can go out result within 10 seconds.
The utility model has temperature to check function, can correct inspection data automatically according to the temperature at inspection scene.
The utility model can store 100,000 inspection datas, can carry out data analysis to these data, can be this
In a little data
Inspection center can be passed back in real time by GPRS technologies by passing computer or printer, testing result.
Claims (3)
- A kind of 1. full spectrum microplate reader for being designed with eight passage cell holders, it is characterised in that：Including tungsten lamp, concave mirror, list Color meter, fiber optic bundle, fiber bench, ELISA Plate and photoelectric sensor, the all-wave length light that tungsten lamp is sent pass through concave mirror deflection angle Enter monochrometer after degree, the monochromatic light of desired wavelength can be adjusted out rapidly in monochrometer, which is by fiber optic bundle Ten uniform light splitting are fine, wherein eight are main fiber, one is used to connect spectrophotometric module for spectrophotometric optical fiber, another Root is used to connect monitoring optical fiber head for monitoring optical fiber；Eight main fiber uniform intervals are fixed in fiber bench, and fiber bench is to monochrome Incidence enters in the ELISA Plate equipped with solution to be measured after light carries out optically focused processing, and the light of some is by the liquid to be checked in ELISA Plate Body absorbs, and the monochromatic light of remainder projects ELISA Plate, and last light, which is incided in the photoelectric sensor of microplate reader, to be changed into Electric signal, the power of the photoelectric sensor electric signal by analyzing microplate reader.
- 2. the full spectrum microplate reader according to claim 1 for being designed with eight passage cell holders, it is characterised in that：The light splitting Luminosity optical fiber is connected on the optical fiber head of spectrophotometric module, which includes cuvette pallet, optical fiber head and rotation Shaft, optical fiber headstock, cuvette pallet are disc, are uniformly spaced with footpath are provided with cuvette thereon, which is eight； Rotation axis is fixedly mounted with the axis position of cuvette pallet bottom, side is coaxially fixedly mounted with optical fiber head below the bottom of cuvette pallet Seat, the optical fiber headstock are formed with groove in the cuvette that corresponding cuvette pallet is installed, to realize that cuvette pallet rotates when ratio Color ware can pass through from the groove internal clearance of optical fiber headstock；Level is fixedly mounted with optical fiber head on the outside of optical fiber headstock, before the optical fiber head The made recess edge of optical fiber headstock of side is coaxially fixedly mounted with a front end collector lens, in the optical fiber headstock of the corresponding front end collector lens Another lateral edges of groove are coaxially fixedly mounted with a rear end collector lens, which connects a full spectrum light electric transducer.
- 3. the full spectrum microplate reader according to claim 1 for being designed with eight passage cell holders, it is characterised in that：The monitoring Optical fiber is connected on monitoring optical fiber head, and the incident light direct irradiation which comes out is in monitoring light path photoelectric sensor It is interior.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721207878.8U CN207366450U (en) | 2017-09-20 | 2017-09-20 | A kind of full spectrum microplate reader for being designed with eight passage cell holders |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721207878.8U CN207366450U (en) | 2017-09-20 | 2017-09-20 | A kind of full spectrum microplate reader for being designed with eight passage cell holders |
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| CN207366450U true CN207366450U (en) | 2018-05-15 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561073A (en) * | 2017-09-20 | 2018-01-09 | 天津瑞泽分析仪器有限公司 | A kind of full spectrum ELIASA for being designed with eight passage cell holders |
| CN112730286A (en) * | 2020-12-23 | 2021-04-30 | 云南省生态环境厅驻昆明市生态环境监测站 | Full-automatic multi-station visible spectrophotometer |
-
2017
- 2017-09-20 CN CN201721207878.8U patent/CN207366450U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561073A (en) * | 2017-09-20 | 2018-01-09 | 天津瑞泽分析仪器有限公司 | A kind of full spectrum ELIASA for being designed with eight passage cell holders |
| CN112730286A (en) * | 2020-12-23 | 2021-04-30 | 云南省生态环境厅驻昆明市生态环境监测站 | Full-automatic multi-station visible spectrophotometer |
| CN112730286B (en) * | 2020-12-23 | 2024-01-12 | 云南省生态环境厅驻昆明市生态环境监测站 | Full-automatic multi-station visible spectrophotometer |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
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| TR01 | Transfer of patent right |
Effective date of registration: 20200116 Address after: 300392 Room 101, 6 doors, building F, Haitai green industry base, no.6, Haitai development road 6, Huayuan Industrial Zone, Binhai New Area, Tianjin Patentee after: Tianer analytical instrument (Tianjin) Co., Ltd Address before: No. 52, Jintang Road, Hedong District, Tianjin, Tianjin Patentee before: TIANJIN RUIZU ANALYTICAL INSTRUMENTS CO., LTD. |