CN104568147A - Monochromator for microplate readers - Google Patents
Monochromator for microplate readers Download PDFInfo
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- CN104568147A CN104568147A CN201510006888.4A CN201510006888A CN104568147A CN 104568147 A CN104568147 A CN 104568147A CN 201510006888 A CN201510006888 A CN 201510006888A CN 104568147 A CN104568147 A CN 104568147A
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- optical fiber
- light
- monochromator
- microplate reader
- monochromatic light
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Abstract
The invention relates to the field of optical colorimetric analysis, in particular to a monochromator for microplate readers. The monochromator comprises a plurality of monochromatic light assemblies and light guide fiber components. The light guide fiber components focus monochromatic light generated by all the monochromatic light assemblies to colorimetric cups corresponding to the microplate readers respectively through different optical paths, and optical signals are converted into electrical signals through a photocell. The light guide fiber components comprise multi-channel optical fiber bundles and optical focus mechanisms. The input ends of the multi-channel optical fiber bundles receive the monochromatic light output by the monochromatic light assemblies, and the optical focus mechanisms are located at the output ends of the multi-channel optical fiber bundles and are focused to the centers of the colorimetric cups of the corresponding channels. By means of the monochromator, two to twelve bundles of monochromatic light of any input wave length and even in energy distribution can be obtained at any time, the response time is short, and no mechanical movement mechanism is needed.
Description
Technical field
What the present invention relates to is a kind of technology of optical colorimetric analysis field, specifically a kind of monochromator for microplate reader.
Background technology
In prior art, the monochromator being applied to microplate reader has two kinds.One makes light source by thermal light sources such as Halogen lamp LEDs, and through several color filter monochromatizing light, be mapped to the end face of 1 ?8 optical fiber, optical fiber components is divided into eight bundles a monochromic beam, through lens, cuvette optically focused on photoelectric commutator, as shown in Figure 1.The import used at home at present or domestic microplate reader, major part is all this structure.The shortcoming of this kind of device is that Overall Power Consumption is large, generate heat high, complex structure, the most of near infrared at more than 800nm of Halogen lamp LED energy distribution, infrared light, bias light intensity is large, improve the requirement to optical filter, convert wavelength simultaneously and need motor and gear train, complex structure, reduce the reliability of instrument, stability.When being used as double UV check, conversion wavelength required time longer 3 ?8 seconds, affect measuring accuracy.
Another kind of monochromator is light source by multiple LED, and LED and corresponding optical filter combine, and provide the various monochromatic light needed for analytical test.It needs a light transmitting fiber equally, a monochromic beam is divided into eight bundles, also needs a stepper motor and gear train to convert monochromatic light, as shown in Figure 2.Therefore, the shortcoming that complex structure, conversion wavelength required time are long is also had.
Through finding the retrieval of prior art, open (bulletin) the day 2014.12.10 of Chinese patent literature CN104198385A, disclose a kind of eight passage switchable light source absorption spectrum pick-up units, comprise eight passage light signal collection plates, eight passage focus lens group, double eight passage light inlet collimator assemblies, notch board, photoswitch, base plate, worm and gear, CD-ROM drive motor, described passage light signal collection plate, eight passage focus lens group, double eight passage light inlet collimator assemblies and notch board connect successively, and described worm and gear is connected on base plate.This Integration ofTechnology degree is high, can detect sample choice lambda1-wavelength position and light intensity according to difference, has single 8 detection fiber joints, can meet Multi-channel optical detection means and high flux optical detection demand.But this technology is only a kind of for selecting the gear train of different beam splitting system, the selection of light source needs motor drag two to arrange collimating mirror group to carry out, front two rows collimating mirror group also needs to connect different beam splitting systems separately just can reach the monochromatic object of switching, and beam splitting system is carried out monochromatic switching and also needed the extra time, if raster pattern is adjusted near ultraviolet infraredly also need the long period.Global facility is more, complex structure, testing requirement more and more rapid when cannot meet multi-wavelength detection in colorimetric analysis.This pick-up unit is not owing to containing beam splitting system and energy management system simultaneously, and the homogeneity of light energy distribution and the dirigibility of control also cannot be ensured.
Summary of the invention
The present invention is directed to prior art above shortcomings, propose a kind of monochromator for microplate reader, can obtain any input wavelength at any time and energy distribution uniform two to ten two monochromic beam, the response time is short and without the need to mechanical motion mechanism.
The present invention is achieved by the following technical solutions:
The present invention includes: some monochromatic light assemblies and optical fiber member, wherein: the monochromatic light that each monochromatic light assembly produces by optical fiber member focuses on the cuvette of corresponding microplate reader respectively by light paths different separately, and by photoelectric cell, light signal is converted to electric signal.
Described optical fiber member comprises: multichannel optical fiber bundle and optical focus mechanism, wherein: the input end of multichannel optical fiber bundle receives the monochromatic light of several monochromatic light assemblies output, and optical focus mechanism is positioned at the output terminal of multichannel optical fiber bundle and will focuses to the center of cuvette corresponding to this road.
Several input ends combination that described multichannel optical fiber bundle is made up of fiber cores and the output terminal combination of corresponding number, wherein: in input end combination, any one group of fiber cores is evenly distributed in all groups of fiber cores in output terminal combination, makes the light of each wavelength be uniform substantially at the energy of eight light-emitting windows.
Described multichannel optical fiber bundle prepares in the following manner: when fibre bundle has N root, one penetrates light inlet fibre-optical splice, the other end of fibre bundle is 1 group with M root and is evenly distributed and passes light-emitting window joint (M is the approximate number of N), then be woven into No. N/M from No. 1 light inlet, this ensure that the light of each wavelength is uniform at the energy of N/M light-emitting window substantially.Hot spot simultaneously in order to make N/M restraint through cuvette is even, requires that the M root optical fiber of each wavelength is uniformly distributed at N/M light-emitting window when weaving.
Technique effect
The present invention can collect two corresponding signals of wavelength simultaneously, interval time with millisecond meter or microsecond meter, and when prior art the double sampling time interval be 3 ~ 8 seconds, therefore the present invention do double UV check more fast, accurately, reliable:
1, the present invention use LED do light source, volume little, generate heat low, the life-span is long;
2, the present invention uses optical fiber member to replace stepping electrode and gear train thereof, and make whole system without movable member, make more compact structure, instrument is more reliable;
3, the present invention realizes modularization, is more conducive to the standardization and seriation design of production, instrument.
Accompanying drawing explanation
Fig. 1 is the monochromator being made light source in prior art by thermal light sources such as Halogen lamp LEDs;
Fig. 2 is that in prior art, multiple LED is the monochromator of light source;
Fig. 3 is structural representation of the present invention;
Fig. 4 be optical fiber member one of them go out the cross sectional representation of optical port;
Fig. 5 is the structural representation of optical fiber member;
Fig. 6 is the schematic diagram of fibre bundle braiding in optical fiber member;
In figure: 1 photoelectric cell, 2 lens, 3 cuvettes, 4 smooth hurdles, 5 lens, 6 eight channel fiber, 7LED, 8LED installing plate, 9LED drive plate, 10 interference filters, 11 gray glasss, 12 light-emitting windows, 13 fiber cores, 14 fibre-optical splices, 15 light inlets, 16 first wallboards, 17 second wallboards, 18 the 3rd wallboards.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
As shown in Figure 3 and Figure 4, the present embodiment comprises: some monochromatic light assemblies 1 and optical fiber member 2, wherein: the monochromatic light that each monochromatic light assembly 1 produces is divided into the cuvette center that some bundles focus on corresponding microplate reader by optical fiber member 2, through lens light gathering on photoelectric cell, realize the conversion of light signal to electric signal.
As shown in Figure 6, the present embodiment goes out optical port for eight light inlets and eight: when arbitrary LED is lit, this monochromatic light is through color filter elements, be incident upon corresponding light transmitting fiber light inlet, through optical fiber components beam splitting, penetrate eight monochromic beams at the light-emitting window of optical fiber components, eight cuvette centers are focused on through lens subassembly, again through lens light gathering on photoelectric cell, finally by operational amplifier, convert light signal to electric signal.Within Millisecond interval time, the signal of two kinds of wavelength light beams is gathered when being used as double UV check.
Described optical fiber member 2 comprises: multichannel optical fiber bundle 6 and optical focus mechanism, wherein: the input end of multichannel optical fiber bundle 6 receives the monochromatic light of several monochromatic light assemblies output, and optical focus mechanism is positioned at the output terminal of multichannel optical fiber bundle and will focuses to the center of cuvette corresponding to this road.
The light inlet mouth of described multichannel optical fiber bundle 6 with go out optical port and also can adopt 12 in other cases.
The monochromatic centre wavelength error that the solid luminescence LED of the monochromatic light assembly 1 in the present embodiment occurs is ± 2nm, and wavelength bandwidth is 8nm, and wavelength coverage contains black light near infrared light, i.e. 340nm ~ 900nm.
Described solid luminescence LED welded and installed is on LED installing plate, and LED drive board provides the electric current required for maximum eight LED with current constant mode, and electric current is linearly adjustable, maximum 64 grades.When measuring beginning, set the electric current required for current LED and export, maximum 8 paths of LEDs can be supplied simultaneously, when needs carry out wavelength switching, flexibly by the channel selecting of digital controllable, the electric current of current LED can be cut off, light the current value of LED needed for setting simultaneously and export, coordinate optical fiber components, the switching time between any wavelength is spaced apart Millisecond.
As shown in Figure 4, the optical focus mechanism described in the present embodiment comprises: some to lens 3 and light hurdle 4.
As long as the present embodiment introducing power supply and control line just can export the monochromatic light of 8 or 12 tunnel energy even on request.
Claims (9)
1. the monochromator for microplate reader, it is characterized in that, comprise: some monochromatic light assemblies and optical fiber member, wherein: the monochromatic light that each monochromatic light assembly produces by optical fiber member focuses on the cuvette of corresponding microplate reader respectively by light paths different separately, and by photoelectric cell, light signal is converted to electric signal;
Described optical fiber member comprises: multichannel optical fiber bundle and optical focus mechanism, wherein: the input end of multichannel optical fiber bundle receives the monochromatic light of several monochromatic light assemblies output, and optical focus mechanism is positioned at the output terminal of multichannel optical fiber bundle and will focuses to the center of cuvette corresponding to this road.
2. the monochromator for microplate reader according to claim 1, it is characterized in that, several input ends combination that described multichannel optical fiber bundle is made up of fiber cores and the output terminal combination of corresponding number, wherein: in input end combination, any one group of fiber cores is evenly distributed in all groups of fiber cores in output terminal combination.
3. the monochromator for microplate reader according to claim 1 and 2, is characterized in that, described optical fiber adopts quartz fibre, glass fibre or its mixing.
4. the monochromator for microplate reader according to claim 1 and 2, is characterized in that, the input end combination of described multichannel optical fiber bundle is combined as eight or 12 with output terminal.
5. the monochromator for microplate reader according to claim 1 and 2, is characterized in that, the light inlet footpath of described multichannel optical fiber bundle is identical with bright dipping bore bore.
6. the monochromator for microplate reader according to claim 2, it is characterized in that, described multichannel optical fiber bundle prepares in the following manner: when fibre bundle has N root, one penetrates light inlet fibre-optical splice, the other end of fibre bundle is 1 group with M root and is evenly distributed and passes light-emitting window joint and the M approximate number that is N, then be woven into No. N/M from No. 1 light inlet, during braiding, the M root optical fiber of each wavelength is uniformly distributed at N/M light-emitting window.
7. the monochromator for microplate reader according to claim 1, is characterized in that, described monochromatic light assembly comprises: solid luminescence LED and corresponding optical filter thereof.
8. the monochromator for microplate reader according to claim 7, it is characterized in that, the monochromatic centre wavelength error that described solid luminescence LED occurs is ± 2nm, and wavelength bandwidth is 8nm, wavelength coverage contains black light near infrared light, i.e. 340nm ~ 900nm.
9. the monochromator for microplate reader according to claim 1, is characterized in that, described optical focus mechanism comprises: some to lens and Guang Lan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510006888.4A CN104568147A (en) | 2015-01-07 | 2015-01-07 | Monochromator for microplate readers |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510006888.4A CN104568147A (en) | 2015-01-07 | 2015-01-07 | Monochromator for microplate readers |
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| Publication Number | Publication Date |
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| CN104568147A true CN104568147A (en) | 2015-04-29 |
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| CN201510006888.4A Pending CN104568147A (en) | 2015-01-07 | 2015-01-07 | Monochromator for microplate readers |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104713647A (en) * | 2015-03-10 | 2015-06-17 | 张美英 | Spectrograph and spectral analysis method |
| CN106168573A (en) * | 2015-09-06 | 2016-11-30 | 深圳雷杜生命科学股份有限公司 | A kind of Systems for optical inspection and include the Blood coagulation instrument of this system |
| CN107561073A (en) * | 2017-09-20 | 2018-01-09 | 天津瑞泽分析仪器有限公司 | A kind of full spectrum ELIASA for being designed with eight passage cell holders |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09126888A (en) * | 1995-10-31 | 1997-05-16 | Kdk Corp | Light source device |
| JPH11108826A (en) * | 1997-09-30 | 1999-04-23 | Kubota Corp | Spectral analyser |
| US20060197958A1 (en) * | 2003-10-17 | 2006-09-07 | Axsun Technologies, Inc. | MEMS Fabry Perot Filter for Integrated Spectroscopy System |
| US20080174768A1 (en) * | 2007-01-18 | 2008-07-24 | Mathias Belz | Self referencing LED detection system for spectroscopy applications |
| CN203069505U (en) * | 2013-01-18 | 2013-07-17 | 苏州慧康电子信息科技有限公司 | Multichannel microplate reader |
| CN103575397A (en) * | 2013-11-22 | 2014-02-12 | 杭州远方光电信息股份有限公司 | Spectral measurement device |
-
2015
- 2015-01-07 CN CN201510006888.4A patent/CN104568147A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09126888A (en) * | 1995-10-31 | 1997-05-16 | Kdk Corp | Light source device |
| JPH11108826A (en) * | 1997-09-30 | 1999-04-23 | Kubota Corp | Spectral analyser |
| US20060197958A1 (en) * | 2003-10-17 | 2006-09-07 | Axsun Technologies, Inc. | MEMS Fabry Perot Filter for Integrated Spectroscopy System |
| US20080174768A1 (en) * | 2007-01-18 | 2008-07-24 | Mathias Belz | Self referencing LED detection system for spectroscopy applications |
| CN203069505U (en) * | 2013-01-18 | 2013-07-17 | 苏州慧康电子信息科技有限公司 | Multichannel microplate reader |
| CN103575397A (en) * | 2013-11-22 | 2014-02-12 | 杭州远方光电信息股份有限公司 | Spectral measurement device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104713647A (en) * | 2015-03-10 | 2015-06-17 | 张美英 | Spectrograph and spectral analysis method |
| CN106168573A (en) * | 2015-09-06 | 2016-11-30 | 深圳雷杜生命科学股份有限公司 | A kind of Systems for optical inspection and include the Blood coagulation instrument of this system |
| CN107561073A (en) * | 2017-09-20 | 2018-01-09 | 天津瑞泽分析仪器有限公司 | A kind of full spectrum ELIASA for being designed with eight passage cell holders |
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Application publication date: 20150429 |