CN105223137B - A kind of optical measuring device for detection of biological samples - Google Patents
A kind of optical measuring device for detection of biological samples Download PDFInfo
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- CN105223137B CN105223137B CN201510683683.XA CN201510683683A CN105223137B CN 105223137 B CN105223137 B CN 105223137B CN 201510683683 A CN201510683683 A CN 201510683683A CN 105223137 B CN105223137 B CN 105223137B
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Abstract
The present invention provides a kind of optical measuring device for detection of biological samples, including being configured as launching the light source assembly of light beam, along light signal transduction component and detection channels component that direction of beam propagation is set gradually is launched to tested biological specimen, the light signal transduction component includes collection fibers beam, light pipe, light splitting fibre bundle；The detection channels component includes being configured as the optical signalling detector placed the Measurement channel of tested biological specimen and set within it and the advance signal process circuit being connected with optical signalling detector output end.The present invention solves the drawbacks of Single wavelength of prior art, single channel are detected, there is provided it is a kind of have multi-wavelength's light may be selected, the optical measuring device of many TCH test channels, the collection that a variety of biological specimens carry the optical signalling of its physiologic information can be completed within the extremely short time, with measurement accuracy height, time of measuring end, measurement efficiency is high, increase the service life the characteristics of, with very high market development value.
Description
Technical field
The invention belongs to field of optical measurements, and in particular to a kind of optical measuring device for detection of biological samples.
Background technology
Clinical diagnostic device field, such as, but not limited to Biochemical Analyzer, ELISA instrument, hemoglobin in vitro
Analyzer etc. needs to be applied to various optical measuring devices.Publication No. CN201707295U and CN101213437B China are specially
A kind of style analytical equipment is disclosed in profit, the device uses halogen light source, and uses filter part, by switching different filters
Optical device obtains the monochromatic light required for style, and Halogen lamp LED that the device is used has short life, and the problems such as difficult positioning, together
When in the presence of the biological specimen that can not be completed in very short time under multiple wavelength optical signalling gather.
Another Publication No. CN20415464U Chinese patent discloses a kind of absorbance measuring device, the feature of the device,
Position is placed including light source, sample and is placed on the sample photodetector that sample places sample absorbance on position for detecting, its
Middle light source includes：Light emitting diode and the drive circuit for providing drive circuit to light emitting diode, the device is in certain journey
The easy problem of aging of Halogen lamp LED is solved on degree, but the device can be only applied to the analyzer of single Air conduct measurement, it is impossible to it is applied to
The occasion of many TCH test channels；There are optical axis eccentricity issues, the especially number when light emitting diode to a certain extent in the other device
When reaching to a certain degree, the hot spot bias for irradiating sample will be more serious, test error easily occurs, final influence measurement essence
Degree.
The content of the invention
In order to solve the above technical problems, the invention provides a kind of optical measuring device for detection of biological samples, it is real
The biological specimen optical signalling of multiple wavelength, multiple TCH test channels is measured in present very short time simultaneously.
To reach above-mentioned purpose, the technical scheme that the present invention is provided is as follows：A kind of optics for detection of biological samples is surveyed
Device is measured, including is configured as launching to tested biological specimen the light source assembly of light beam, the light source assembly is independently arranged
Two groups, respectively the first light source assembly and secondary light source component, the hair of both first light source assembly and secondary light source component
Light spectrum is not overlapping；
It also includes the light signal transduction component and detection channels component set gradually along transmitting direction of beam propagation, institute
Stating light signal transduction component includes
- collection fibers beam, its two ends are each configured with several first incidence ends and first exit end；
- light pipe, it is the column structure that two ends are each configured with second incidence end and second exit end, institute
State the second exit end and docked with the first exit end and developed；
- have and converge the composite fiber beam that light and light splitting combine, it is configured with several the 3rd incidence ends, several the 3rd go out
End and a common end are penetrated, the common end is docked with the second incidence end develops；
- optical mirror, it, which is docked, develops the second exit end setting；
The beam emissions end of first light source assembly is docked with composite fiber beam, the light beam hair of the secondary light source component
End is penetrated to dock with collection fibers beam；
The detection channels component includes,
- port number and the 3rd exit end quantity identical Measurement channel, the entrance of the Measurement channel is with the 3rd
Exit end is docked, the setting tested biological specimen in it；
- and the Measurement channel quantity identical optical signalling detector, it is described along the direction of propagation of the transmitting light beam
Optical signalling detector is arranged at the downstream of tested biological specimen；
- advance signal process circuit, its input connects the output end of each signal sensor.
In the preferred embodiment of the present invention, further comprise first light source assembly and second light source assembly
Structure is identical, and it includes
- light emitting diode matrix, it includes at least four semiconductor light-emitting-diodes；
The drive circuit that-driving the semiconductor light-emitting-diode is closed or opened.
In the preferred embodiment of the present invention, further comprise that first light source assembly includes,
- light emitting diode matrix, it includes at least four semiconductor light-emitting-diodes；
The drive circuit that-driving the semiconductor light-emitting-diode is closed or opened；
The secondary light source component includes the switch that Halogen lamp LED and the driving Halogen lamp LED are closed or opened.
In the preferred embodiment of the present invention, further comprise along the direction of propagation of the transmitting light beam, it is each described
The downstream of semiconductor light-emitting-diode is equipped with one first optical lens, and first incidence end is arranged on the first optical lens
On focus point, along the direction of propagation of the transmitting light beam, the upstream of each tested biological specimen is equipped with one second optics
Lens.
In the preferred embodiment of the present invention, further comprise along the direction of propagation of the transmitting light beam, it is each described
The downstream of first optical lens is equipped with an interferometric filter.
The beneficial effects of the invention are as follows:The present invention solve the drawbacks of Single wavelength of prior art, single channel are detected there is provided
It is a kind of have multi-wavelength's light may be selected, the optical measuring device of many TCH test channels, can within the extremely short time simultaneously collection
The optical signalling for carrying its physiologic information of a variety of biological specimens, optical signalling obtains after processing and represents biological specimen physiology
The measurement data of information, after whole measurement apparatus use while measurement accuracy is improved, with that can shorten time of measuring, is carried
The characteristics of high measurement efficiency, extension measurement apparatus service life, with very high market development value.
Brief description of the drawings
Technical scheme in technology in order to illustrate the embodiments of the present invention more clearly, in being described below to embodiment technology
The required accompanying drawing used is briefly described, it should be apparent that, drawings in the following description are only some realities of the present invention
Example is applied, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to these accompanying drawings
Obtain other accompanying drawings.
Fig. 1 is the structural representation of the preferred embodiment of the present invention；
Fig. 2 is the switch control time sequence schematic diagram of the semiconductor light-emitting-diode of the preferred embodiment of the present invention；
Fig. 3 is the structural representation of the collection fibers beam of the preferred embodiment of the present invention；
Fig. 4 is the distribution schematic diagram of the incidence end optical fiber wire of the collection fibers beam of the preferred embodiment of the present invention；
Fig. 5 is the structural representation of the light splitting fibre bundle of the preferred embodiment of the present invention；
Fig. 6 is the theory diagram of advance signal process circuit；
Fig. 7 is the structural representation of second embodiment of the invention；
Fig. 8 is the curve of spectrum schematic diagram of optical mirror in second embodiment of the invention；
Fig. 9 is the structural representation of third embodiment of the invention
Wherein, 2- biological specimens, the optical lenses of 4- first, 6- interferometric filters, 7- optical fiber wires, the optical lens of 8- second
Mirror；
1- light source assemblies, the light source assemblies of 1a- first, 1b- secondary light source components, 11- semiconductor light-emitting-diodes, 12- drives
Dynamic circuit, 13- Halogen lamp LEDs；
3- light signal transduction components, 31- collection fibers beams, the incidence ends of 31a- first, the exit ends of 31b- first；
32- light pipes, the incidence ends of 32a- second, the exit ends of 32b- second；33- light splitting fibre bundles, the incidence ends of 33a- the 3rd,
The exit ends of 33b- the 3rd；34- composite fiber beams, 34a- is held jointly；35- optical mirrors；
5- detection channels components, 51- optical signalling detectors, 52- advance signal process circuits.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Embodiment one
As shown in figures 1 to 6, a kind of optical measuring device for detection of biological samples, including quilt are disclosed in the present embodiment
It is configured to pass to the optical signal that tested biological specimen 2 launches the light source assembly 1 of light beam, edge transmitting direction of beam propagation is set gradually
Guide assembly 3 and detection channels component 5, be provided with detection channels component 5 TCH test channel that is used for placing tested biological specimen 2 and
It is arranged on the optical signalling detector 51 in TCH test channel.Light source assembly 1 launches light beam, and transmitting light beam is through light signal transduction component
3 conduction to direct irradiation configures the tested biological specimen 2 in detection channels component 5, and biological specimen 2 has certain optics in itself
Characteristic, the optical effect of extinction or scattering etc. is carried out to transmitting light beam, and the light beam after the optical effect of biological specimen 2 is carried
The biological information that biological specimen 2 is characterized, the light beam for carrying biological information enters optical signalling detector 51, completes biological specimen
2 information gathering.
Wherein, light source assembly 1 includes the hair in light emitting diode matrix and drive circuit 12, the preferred embodiment of the present invention
Light diode array includes at least one semiconductor light-emitting-diode 11, constitutes the pole of semiconductor light emitting two of light emitting diode matrix
The different model that is multiple, being multiple rows of setting for the same model that the quantity of pipe 11, which can be one, can be a row is set
Multiple (multiple models that wherein same row is set are identical), a row set the quantity of same model semiconductor light-emitting-diode 11 according to
Depending on measuring the luminous intensity size needed, depending on the number of wavelengths that the row of multiple rows of setting is needed according to measurement, semiconductor light emitting two
A kind of model correspondence of pole pipe 11 sends a kind of light of wavelength, and the model of semiconductor light-emitting-diode 11 is preferred to use with one
Determine the monochromatic LED of narrow-band spectrum, can also be from the White LED type for sending mixed spectra the need for being measured certainly according to reality
Number.Fig. 1 shows the structure chart of the light source assembly with four semiconductor light-emitting-diodes 11, wherein each semiconductor light emitting two
A kind of model of the correspondence of pole pipe 11.
Drive circuit 12 controls lamping on and off for the semiconductor light-emitting-diode 11 in light emitting diode matrix, a certain
The semiconductor light-emitting-diode 11 that moment can only have a kind of wavelength is opened, and shows that drive circuit controls four semiconductors in Fig. 2
The control sequential figure that light emitting diode 11 is lamped on and off, four semiconductor light-emitting-diodes 11 are in the control of drive circuit 12
Lamped on and off successively in turn under sequence, the cycle of preferred switch control time sequence is 50ms, each semiconductor light emitting two in this implementation
The lighting time of pole pipe 11 is 6.25ms, to realize the purpose measured within the extremely short time.
As a further improvement on the present invention, in order to improve levels of light energy, along it is described transmitting light beam the direction of propagation,
The downstream of each semiconductor light-emitting-diode 11 is equipped with one first optical lens 4, and the first optical lens 4 is used to focus on half
The light beam of conductor light emitting diode 11, it is ensured that more light enter measurement apparatus.
As a further improvement on the present invention, in order to improve the monochromaticjty level of light, along the propagation of the transmitting light beam
Direction, is equipped with an interferometric filter 6, herein the preferred narrow band of interferometric filter 6 in the downstream of each first optical lens 4
Interference formula optical filter, its half-wave bandwidth, which is generally less than, is equal to 12nm, and the use of interferometric filter 6 can be to partly leading under the passage
The spectrum of body light emitting diode 11 is filtered processing, removes unexpected wavelength, and the optics background depth of lifting device, carries
The optical s/n ratio of high device, the final detection accuracy for improving biological specimen.
Focusing of the light beam that semiconductor light-emitting-diode 11 is sent through the first optical lens 4, after the filtering of interferometric filter 6
Launch into light signal transduction component 3.
In order to complete the light conduction of the transmitting light beam of light source assembly 1, the light signal transduction component 3 of the present embodiment includes setting successively
Collection fibers beam 31, light pipe 32 and the light splitting fibre bundle 33 put,
Wherein, collection fibers beam 31 is a kind of light with several first incidence ends 31a, first exit end 31b
Fibre bundle is led, in order to ensure the optical fiber of more semiconductor light-emitting-diodes 11 enters collection fibers beam 33, the present invention is excellent
The first incidence end 31a is selected to be arranged on the focus point of the first optical lens 4.
Multiple optical fiber wires 7, first incidence end are uniformly distributed in first incidence end 31a of collection fibers beam 31
31a at least corresponds to a semiconductor light-emitting-diode 11, when the situation that the light energy of a semiconductor light-emitting-diode 11 is sufficient
Under, an one first incidence end 31a of correspondence of semiconductor light-emitting-diode 11, when the semiconductor light-emitting-diode 11 of some wavelength
Light energy it is not enough in the case of, suitably increase the number of the semiconductor light-emitting-diode 11 of this wavelength, enter the to improve
One incidence end 31a light energy, now multiple semiconductor light-emitting-diodes 11 of first incidence end 31a correspondence, these same ripples
Long semiconductor light-emitting-diode 11 is distributed around corresponding first transmitting terminal 31a central shaft.Several first incidence ends 31a
The light that semiconductor light-emitting-diode 11 is sent collects, and the conduction through optical fiber wire 7 to a first exit end 31b is launched
Go out, into light pipe 32,32 pairs of light of light pipe carry out hot spots homogenization conduction, wherein, the quantity of optical fiber wire 7 is according to this
Depending on the luminous intensity size of passage lower semiconductor light emitting diode 11, when lighting for the passage lower semiconductor light emitting diode 11
When intensity is weaker, then the quantity of correspondence optical fiber wire 7 can be properly increased, to increase the luminous flux into device.
Wherein, light pipe 32 is a kind of utilization optical total-reflection principle and the column structure that is made, column structure include but
Hexagon column structure, reducing column structure, the cylindrical structure of composite parabolic are not limited to, present invention preferably employs equilateral
Hexagon column structure, in addition, the of clear aperature and light splitting fibre bundle 33 as the first exit end 31b of collection fibers beam 31
When three incidence end 33a clear aperature is inconsistent, light pipe 32 is preferred to use column structure, it is ensured that the of light pipe 32
Two incidence end clear aperatures are slightly larger than the first exit end 31b of collection fibers beam 31 clear aperature, and the second of light pipe 32 goes out
Penetrate clear aperature of the end clear aperature slightly larger than the 3rd incidence end 33a of light splitting fibre bundle 33.
The two ends of light pipe 32 are each configured with a second incidence end 32a and a second exit end 32b, described second
Incidence end 32a is docked with the first exit end 31b and developed, and docking, which is developed, can reduce space, prevent optical fiber from leaking injection from gap
Go, so as to influence light energy.Light is launched in its second exit end 32b formation uniform light spots, entered after light pipe 32
Enter light splitting fibre bundle 33.
Wherein, light splitting fibre bundle 33 is a kind of to carry a 3rd incidence end 33a and several the 3rd exit end 33b
Fibre-optic bundle, the 3rd incidence end 33a is docked with the second exit end 33b of light pipe 32 and developed, and docking, which is developed, can reduce sky
Gap, prevents optical fiber from leaking from gap to be shot out, so as to influence light energy.3rd incidence end 33a of light splitting fibre bundle 33 uniformly divides
The multiple optical fiber wires 7 of cloth, these optical fiber wires 7 will be divided into uniform light spots many parts of subdivision light, and each subdivision light will
It is independent to launch from a 3rd exit end 33b of light splitting fibre bundle 33, into detection channels component 5.
In order to complete the reception and detection of optical signal, the detection channels component 5 of the present embodiment includes Measurement channel, is arranged on
Optical signalling detector 51 and advance signal process circuit 52 in Measurement channel,
Wherein, the quantity of the Measurement channel of configuration is identical with the 3rd exit end 33b quantity, the entrance of each Measurement channel
Correspond to and docked with a 3rd exit end 33b, tested biological specimen 2 is placed in Measurement channel；
The quantity of the optical signalling detector 51 of configuration is identical with the port number of Measurement channel, is configured in a Measurement channel
One optical signalling detector 51, and along the direction of propagation of the transmitting light beam, the optical signalling detector in same passage
51 are arranged at the downstream of tested biological specimen 2.In single Measurement channel, from the 3rd exit end of light splitting fibre bundle 33
The light that 33b is emitted enters in Measurement channel, and biological specimen 2 is irradiated in Measurement channel, and optical signalling detector 51 is adopted
Collect the optical signalling of the biological specimen 2 under light irradiation, the signal output of collection to advance signal process circuit 52.The present invention's
Optical signalling detector 51 includes but is not limited to silicon photo diode, photocell or photomultiplier, preferably silicon photo diode.
Advance signal process circuit 52, its input connects the output end of each signal sensor 51, as shown in fig. 6,
The biological specimen optical signalling that 52 pairs of collections of advance signal process circuit of the present invention come carries out opto-electronic conversion successively, and light intensity is turned
Change electric signal into, then electric signal is filtered successively, amplified and the processing such as analog-to-digital conversion, be finally uploaded to digital quantity preposition
At the control terminal of signal processing circuit 52, data extraction, calculating and the output report of the physiologic information for carrying out biological specimen 2
Reason.
As a further improvement on the present invention, in order to improve levels of light energy, along it is described transmitting light beam the direction of propagation, often
The upstream of the individual tested biological specimen 2 is equipped with one second optical lens 8, and the second optical lens 8 is used to focus on subdivision light,
Ensure more light irradiation biological specimens 2.
Embodiment two
Difference of the embodiment two compared to embodiment one is：The test of greater number of biological specimen, which can be completed, to be needed
Ask.
As shown in figures 1-8, a kind of optical measuring device for detection of biological samples, including quilt are disclosed in the present embodiment
It is configured to launch light beam and work the first independent light source assembly 1a and secondary light source component 1b, edge hair to tested biological specimen
Light signal transduction component 3 and detection channels component 5 that the irradiating light beam direction of propagation is set gradually.Provided with use in detection channels component 5
In the optical signalling detector 51 placed the TCH test channel of tested biological specimen 2 and be arranged in TCH test channel.Light source assembly 1 is sent out
Irradiating light beam, transmitting light beam is conducted to direct irradiation through light signal transduction component 3 configures the tested biology in detection channels component 5
There is certain optical characteristics in sample 2, biological specimen 2, the optical effect of extinction or scattering etc., warp are carried out to transmitting light beam in itself
Light beam after the optical effect of biological specimen 2 carries the biological information that biological specimen 2 is characterized, and the light beam for carrying biological information enters
Enter optical signalling detector 51, complete the information gathering of biological specimen 2.
Wherein, the structure of the first light source assembly 1a and second light source assembly 1b are identical, specific as follows：
First light source assembly 1a includes the hair in light emitting diode matrix and drive circuit 12, second embodiment of the invention
Light diode array includes at least four semiconductor light-emitting-diodes 11, constitutes the pole of semiconductor light emitting two of light emitting diode matrix
The quantity of pipe 11 can be two, four, can be the same model that a row is set different shaped that is multiple, being multiple rows of setting
Number multiple (multiple models that wherein same row is set are identical), a row sets the quantity of same model semiconductor light-emitting-diode 11
Depending on the luminous intensity size needed according to measurement, the row of multiple rows of setting is according to depending on the number of wavelengths for measuring needs, and semiconductor is sent out
A kind of model correspondence of optical diode 11 sends a kind of light of wavelength, and the model of semiconductor light-emitting-diode 11 is preferred to use tool
There is the monochromatic LED of certain narrow-band spectrum, can also be from the White LED for sending mixed spectra the need for being measured certainly according to reality
Model.Fig. 1 shows the structure chart of the light source assembly with four semiconductor light-emitting-diodes 11, wherein each semiconductor light emitting
A kind of model of the correspondence of diode 11.
In addition, both first light source assembly 1a and secondary light source component 1b of the present invention luminescent spectrum is not overlapping.
Drive circuit 12 controls lamping on and off for the semiconductor light-emitting-diode 11 in light emitting diode matrix, a certain
The semiconductor light-emitting-diode 11 that moment can only have a kind of wavelength is opened, and shows that drive circuit controls four semiconductors in Fig. 2
The control sequential figure that light emitting diode 11 is lamped on and off, four semiconductor light-emitting-diodes 11 are in the control of drive circuit 12
Lamped on and off successively in turn under sequence, the cycle of preferred switch control time sequence is 50ms, each semiconductor light emitting two in this implementation
The lighting time of pole pipe 11 is 6.25ms, to realize the purpose measured within the extremely short time.
As a further improvement on the present invention, in order to improve levels of light energy, along it is described transmitting light beam the direction of propagation,
The downstream of each semiconductor light-emitting-diode 11 is equipped with one first optical lens 4, and the first optical lens 4 is used to focus on half
The light beam of conductor light emitting diode 11, it is ensured that more light enter measurement apparatus.
As a further improvement on the present invention, in order to improve the monochromaticjty level of light, along the propagation of the transmitting light beam
Direction, is equipped with an interferometric filter 6, herein the preferred narrow band of interferometric filter 6 in the downstream of each first optical lens 4
Interference formula optical filter, its half-wave bandwidth, which is generally less than, is equal to 12nm, and the use of interferometric filter 6 can be to partly leading under the passage
The spectrum of body light emitting diode 11 is filtered processing, removes unexpected wavelength, and the optics background depth of lifting device, carries
The optical s/n ratio of high device, the final detection accuracy for improving biological specimen.
The light beam that first light source assembly 1a and secondary light source component 1b are each sent is poly- through the first optical lens 4 successively
Burnt, the laggard optical signal conducting subassembly 3 of filtering of interferometric filter 6.
In order to complete the light conduction of the transmitting light beam of light source assembly 1, the light signal transduction component 3 of the present embodiment includes and first
Light source assembly 1a docking composite fiber beam 34, with the secondary light source component 1b collection fibers beams 31 docked and two ends difference
The light pipe 32 docked with collection fibers beam 31 and composite fiber beam 34, the two ends of light pipe 32 are each configured with one second and entered
Penetrate the second exit end 32b of 32a and one, end, the second exit end 32b is provided with an optical mirror 35, the second incidence end 32a with
Composite fiber beam 34 is docked, and the second exit end 32b is docked with collection fibers beam 31.
Wherein, composite fiber beam 34 of the invention is by with collecting collecting for beam functions according to specific manufacture craft
Fibre bundle and light splitting fibre bundle with light splitting function, which are synthesized, to be wholely set, and it is made up of multi-channel optical fibre branch, different optical fiber
Branch is used to realize fiber collection function and light splitting function.Composite fiber beam 34 after making is a kind of optical fiber more than one turn
Beam, it is configured with several the 3rd incidence end 33a, several the 3rd exit end 33b and a common end 34a, and common end 34a converges
The optical fiber wire for having the 3rd incidence end 33a and the 3rd exit end 33b is closed, wherein the 3rd exit end 33b optical fiber wires are altogether
Inner ring with end is uniformly distributed, and the 3rd incidence end 33a optical fiber wire is uniformly distributed outer shroud.
Several transmitting terminals of the 3rd incidence end 33a respectively with the first light source assembly 1a are docked, several the 3rd exit ends
33b is docked with detection channels component, and common end 34a is developed with the second incidence end 32a to be docked.
Collection fibers beam 31, its two ends are each configured with several the first incidence end 31a and a first exit end 31b,
The first exit end 31b is developed with the second exit end 32b by optical mirror 35 and docked, several first incidence ends 31a
The transmitting terminal with secondary light source component 1b is docked respectively.
The optical mirror 35 of the present invention has the special curve of spectrum, and the reflectance spectrum of optical mirror 35 includes first
Light source assembly 1a luminescent spectrum, the transmitted spectrum of optical mirror 35 includes secondary light source component 1b luminescent spectrum, such as Fig. 8
Shown, the optical mirror 35 that the present invention is used, its curve of spectrum is controlled 5% in the light transmittance of spectrum line of demarcation left-half
Below, it is therefore an objective to prevent first group of light source assembly 1a light-emitting line from being transmitted away from optical mirror 35；Its curve of spectrum exists
The light transmittance of spectrum line of demarcation right half part is controlled more than 95%, it is therefore an objective to allow secondary light source component 1b light-emitting line to transmit
Cross optical mirror 35 and enter light pipe 32.
Based on the said structure of light signal transduction component 3, after optical mirror 35 is set, the first light source assembly 1a and the
The propagation path that light beam is sent in two light source assembly 1b is as follows：First light source assembly 1a light beam by composite fiber beam 34 conduct to
After 3rd exit end 33b, imported from the second incidence end 32a of light pipe 32 in light pipe 32, travel to the second of light pipe 32
Reflected during exit end 32b by optical mirror 35, light beam turns back from composite fiber beam 34 in light pipe 32 is reverse after reflection
The 3rd exit end 33b launch, into detection channels component 5；Secondary light source component 1b light beam is caught fibre bundle 31
Conduct to the first exit end 31b, imported through optical mirror 35 from the second exit end 32b in light pipe 32, in leaded light
Launch during the 3rd exit end 33b that composite fiber beam 34 is traveled in pipe 32 into detection channels component 5.
In order to complete the reception and detection of optical signal, the detection channels component 5 of the present embodiment includes Measurement channel, is arranged on
Optical signalling detector 51 and advance signal process circuit 52 in Measurement channel,
Wherein, the quantity of the Measurement channel of configuration is identical with the 3rd exit end 33b quantity, the entrance of each Measurement channel
Correspond to and docked with a 3rd exit end 33b, tested biological specimen 2 is placed in Measurement channel；
The quantity of the optical signalling detector 51 of configuration is identical with the port number of Measurement channel, is configured in a Measurement channel
One optical signalling detector 51, and along the direction of propagation of the transmitting light beam, the optical signalling detector in same passage
51 are arranged at the downstream of tested biological specimen 2.In single Measurement channel, from the 3rd exit end of light splitting fibre bundle 33
The light that 33b is emitted enters in Measurement channel, and biological specimen 2 is irradiated in Measurement channel, and optical signalling detector 51 is adopted
Collect the optical signalling of the biological specimen 2 under light irradiation, the signal output of collection to advance signal process circuit 52.The present invention's
Optical signalling detector 51 includes but is not limited to silicon photo diode, photocell or photomultiplier, preferably silicon photo diode.
Advance signal process circuit 52, its input connects the output end of each signal sensor 51, as shown in fig. 6,
The biological specimen optical signalling that 52 pairs of collections of advance signal process circuit of the present invention come carries out opto-electronic conversion successively, and light intensity is turned
Change electric signal into, then electric signal is filtered successively, amplified and the processing such as analog-to-digital conversion, be finally uploaded to digital quantity preposition
At the control terminal of signal processing circuit 52, data extraction, calculating and the output report of the physiologic information for carrying out biological specimen 2
Reason.
As a further improvement on the present invention, in order to improve levels of light energy, along it is described transmitting light beam the direction of propagation, often
The upstream of the individual tested biological specimen 2 is equipped with one second optical lens 8, and the second optical lens 8 is used to focus on subdivision light,
Ensure more light irradiation biological specimens 2.
Embodiment three
Embodiment three is different from being only that for embodiment two：Secondary light source component 1b structure is different from the first light source assembly
1a, it is specific as follows：
First light source assembly 1a structure and associated description be the same as Example two, is not being repeated herein.
Secondary light source component 1b includes the switch that Halogen lamp LED 13 and the driving Halogen lamp LED 13 are closed or opened, and uses halogen
Plain lamp 13 can solve the problem that the problem of semiconductor light-emitting-diode 11 can not produce ultraviolet spectra, can expand the present apparatus after use
Spectrum uses scope, to meet the biological specimen testing requirement of ultraviolet wavelength.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or using the present invention.
A variety of modifications to these embodiments will be apparent for those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, it is of the invention
The embodiments shown herein is not intended to be limited to, and is to fit to and principles disclosed herein and features of novelty phase one
The most wide scope caused.
Claims (5)
1. a kind of optical measuring device for detection of biological samples, including be configured as launching light beam to tested biological specimen
Light source assembly, it is characterised in that：The light source assembly is two groups be independently arranged, respectively the first light source assembly and secondary light source
Component, the luminescent spectrum of both first light source assembly and secondary light source component is not overlapping；
It also includes the light signal transduction component and detection channels component set gradually along transmitting direction of beam propagation, the light
Signal transduction component includes
- collection fibers beam, its two ends are each configured with several first incidence ends and first exit end；
- light pipe, it is that two ends are each configured with the column structure of second incidence end and second exit end, described the
Two exit ends are docked with the first exit end and developed；
- composite fiber the beam that there is remittance light and light splitting to combine, it is configured with several the 3rd incidence ends, several the 3rd exit ends
With a common end, the common end is docked with the second incidence end develops；
- optical mirror, it, which is docked, develops the second exit end setting；The reflectance spectrum of the optical mirror includes first
The luminescent spectrum of light source assembly, the transmitted spectrum of optical mirror includes the luminescent spectrum of secondary light source component；
The beam emissions end of first light source assembly is docked with composite fiber beam, the beam emissions end of the secondary light source component
Docked with collection fibers beam；
The detection channels component includes,
- port number and the 3rd exit end quantity identical Measurement channel, the entrance of the Measurement channel with the 3rd outgoing
End docking, the setting tested biological specimen in it；
- and the Measurement channel quantity identical optical signalling detector, along the direction of propagation of the transmitting light beam, the optics
Signal sensor is arranged at the downstream of tested biological specimen；
- advance signal process circuit, its input connects the output end of each signal sensor.
2. a kind of optical measuring device for detection of biological samples according to claim 1, it is characterised in that：Described
The structure of one light source assembly and second light source assembly is identical, and it includes
- light emitting diode matrix, it includes at least four semiconductor light-emitting-diodes；
The drive circuit that-driving the semiconductor light-emitting-diode is closed or opened.
3. a kind of optical measuring device for detection of biological samples according to claim 1, it is characterised in that：Described
One light source assembly includes,
- light emitting diode matrix, it includes at least four semiconductor light-emitting-diodes；
The drive circuit that-driving the semiconductor light-emitting-diode is closed or opened；
The secondary light source component includes the switch that Halogen lamp LED and the driving Halogen lamp LED are closed or opened.
4. a kind of optical measuring device for detection of biological samples according to Claims 2 or 3, it is characterised in that：Edge
The direction of propagation of the transmitting light beam, the downstream of each semiconductor light-emitting-diode is equipped with one first optical lens, institute
State the first incidence end to be arranged on the focus point of the first optical lens, along the direction of propagation of the transmitting light beam, each quilt
The upstream for surveying biological specimen is equipped with one second optical lens.
5. a kind of optical measuring device for detection of biological samples according to claim 4, it is characterised in that：Described in
Launch the direction of propagation of light beam, the downstream of each first optical lens is equipped with an interferometric filter.
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| CN109085673B (en) * | 2017-06-14 | 2020-05-22 | 重庆海扶医疗科技股份有限公司 | Light transmission assembly and photoluminescence detection system |
| CN110470390B (en) * | 2018-05-10 | 2020-12-18 | 重庆海扶医疗科技股份有限公司 | Compression-resistant optical fiber for detecting ultrasonic sonoluminescence signal |
| CN111103246A (en) * | 2018-10-26 | 2020-05-05 | 中国科学院长春光学精密机械与物理研究所 | Light splitting photometer |
| CN111094941B (en) * | 2019-11-29 | 2022-07-29 | 深圳市汇顶科技股份有限公司 | PPG circuit, biological feature detection device and biological feature detection method |
| WO2021207871A1 (en) * | 2020-04-13 | 2021-10-21 | 深圳迈瑞生物医疗电子股份有限公司 | Anti-interference detection method and sample analyzer |
| CN112304915B (en) * | 2020-10-29 | 2021-05-04 | 苏州雅睿生物技术有限公司 | Real-time fluorescence detection optical system and real-time fluorescence quantitative PCR instrument |
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