CN109932323A - A kind of portable spectroscopic system of multichannel lighted based on timesharing and its detection method - Google Patents
A kind of portable spectroscopic system of multichannel lighted based on timesharing and its detection method Download PDFInfo
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Abstract
The invention discloses a kind of portable spectroscopic system of multichannel lighted based on timesharing and its detection methods.Spectroscopic system of the invention substitutes grating using linear filtering piece, and space required for input optical dispersion is unfolded in grating is greatly saved and increases the portability of spectroscopic system compared with grating spectrograph；Technology is lighted using timesharing, it allows multiple light sources successively to flash in sample cell, measurement is completed in time several seconds, compared with grating spectrograph, increase spectroscopic system measuring speed and test sample quantity measured every time, substantially increase the working efficiency of spectroscopic system；Spectroscopic system unifies fiber optic splitter using n, be transferred to the information light of the test sample in multiple channels can on a linear CCD element, compared with grating spectrograph, simplifies experimental implementation, further save space, increase portability；Cost of manufacture and manufacture difficulty of the present invention substantially reduce.
Description
Technical field
The present invention relates to spectrum detection techniques, and in particular to a kind of portable spectroscopic system of multichannel lighted based on timesharing and
Its detection method.
Background technique
Light action can generate the multiple spectrums phenomenons such as the transmission, scattering, Absorption and emission spectra of light in substance.According to spectrum
Characteristic can obtain the relevant informations such as ingredient, the content of studied substance, therefore spectrum detection technique is widely used in biochemistry
The numerous areas such as detection, biomedicine, environmental monitoring, health quarantine, food pharmaceutical, industrial and agricultural production, astronomical observation.Spectrometer
It is one of the means for realizing spectral detection, it is strong using the photodetectors such as photomultiplier tube acquisition spectral line different wave length position
The scientific instrument of degree.Spectrometer is generally by entrance slit, dispersion system, imaging system and exit slit composition.By dispersion element
Difference can be divided into prism spectrometer, grating spectrograph and interference spectroscope etc..
Current various spectroscopic systems are that specific light source shines, through being input in spectrometer after measured matter.Grating will
The optical signal that input is come in carries out spatial dispersion, on the detectors such as light beam irradiation covering CCD after expansion, will finally be examined by computer
It surveys the collected spectral information of device and is depicted as spectrum picture.Such spectrometer is used in many necks such as each bioid inspection
Domain, but because grating needs spatial dispersion, the volume of this kind of spectrometer is relatively large, and making it in use has many limits
System.
It is in place of problem and shortage existing for existing spectrometer:
(1) existing grating spectrograph, at high cost, manufacturing conditions are harsh；
(2) existing grating spectrograph, volume is relatively large, and use environment, place to use are limited；
(3) existing grating spectrograph, measurement method is cumbersome, and operating method is relative complex, and measuring speed is very slow.
Summary of the invention
For the above problems of the prior art, the invention proposes a kind of multichannel lighted based on timesharing is portable
Spectroscopic system and its detection method, device is simple, and detection speed is fast.
An object of the present invention is to provide a kind of portable spectroscopic systems of the multichannel lighted based on timesharing.
The portable spectroscopic system of multichannel lighted based on timesharing of the invention includes: control circuit, light source, sample stage, ratio
Color ware, n unification fiber optic splitter, linear filtering piece, linear CCD element and computer；Wherein, sample stage is transversely set gradually
There is n sample cell；It is offered on each sample cell along the light hole perpendicular to sample stage transverse direction, light hole runs through sample
The front and rear surfaces of product slot；Control circuit connects n light source, and light source uses white light；Each light source corresponds to a sample cell, fitting
The front surface of sample cell, the corresponding light hole of face；It is the fiber optic bundle that multifiber is formed, fiber optic bundle that n, which unifies fiber optic splitter,
One end bonding be bonded a convex lens in the rear end of light-emitting window, the other end of fiber optic bundle is opposite with sample cell as light-emitting window
N strands should be separated into, is made of again multifiber per one fiber optic bundle, in this, as light inlet；In the front end of each light inlet
It is bonded a convex lens, is separately mounted to the light hole of the rear surface of corresponding sample cell；Linear filtering piece is flattened on linear CCD
On the photosensitive surface of element；The linear CCD element for being pasted with linear filtering piece is fixed in front of light-emitting window, photosensitive surface with go out
Optical port horizontal alignment, so that the lucky fully wrapped around firmly photosensitive surface of the emergent light of n unification fiber optic splitter；Linear CCD element connects
It is connected to computer；The n cuvettes for filling test sample are inserted into respectively in corresponding sample cell, by control circuit, control n
Light source is successively flashed according to chronological order；After the white light that light source issues is through the test sample in cuvette, information light warp
Convex lens convergence is transmitted to light-emitting window by fiber optic bundle from the light inlet of n unification fiber optic splitter；The diverging that fiber optic bundle issues
Light becomes less parallel light after the converging action of light-emitting window convex lens, is finally covered on the photosensitive surface of linear CCD element
Linear filtering on piece；After information light penetrates linear filtering piece, sequentially received by linear CCD element according to chronological order,
And it is transmitted to computer, computer successively obtains the spectrogram of the test sample in each sample cell according to chronological order
Picture, wherein the natural number that n is >=2.
Filter plate is the optical device for choosing required radiation wave band.Linear filtering piece has saturating in different spatial
The characteristic of different wave length coloured light is crossed, its each different spatial position corresponds to the narrow-band filtering of different light transmission passage spectral coverages
Piece.Spectrum channel of this linear filtering piece at different spaces only allows optical signal to pass through in specific wave band, and deviates this
Two optical signals other than a wave band are prevented from, and the passband in each of which channel is the 5% or less of central wavelength.
Control circuit includes power supply, switch and single-chip microcontroller；Wherein, power supply is connect by switch with single-chip microcontroller, and single-chip microcontroller mentions
The pin of low and high level can be exported for n, each pin is correspondingly connected with a light source.By lower switch, power supply is to monolithic
Machine is powered, and single-chip microcontroller controls n light source respectively and sequentially flashes.
Light source uses white light LEDs light bulb.
Control circuit and n light source setting are on circuit boards.
Sample stage uses light-proof material that is easily molded, not reflecting specific band coloured light, such as plastics, aluminium alloy or timber
Deng.
It is another object of the present invention to provide a kind of detections of portable spectroscopic system of the multichannel lighted based on timesharing
Method.
The detection method of the portable spectroscopic system of multichannel lighted based on timesharing of the invention, comprising the following steps:
1) booting enters self-test and verification demarcation flow, to be modified to different sample cells:
A) all samples slot, the portable spectroscopic system booting of multichannel are emptied；
B) k-th of sample cell in n sample cell is selected as verification slot, verification slot is arbitrarily chosen, k ∈ 1,2 ... ...,
n；
C) by control circuit, n light source of control is successively flashed according to chronological order, and the white light that light source issues passes through
After sample cell, information light is converged through light inlet convex lens, by the light inlet of n unification fiber optic splitter, is transmitted to by fiber optic bundle
Light-emitting window, the diverging light that fiber optic bundle issues become less parallel light after light-emitting window convex lens, are finally covered on linear CCD element
Photosensitive surface on linear filtering on piece, information light penetrate linear filtering piece after, it is suitable according to time order and function by linear CCD element
Sequence sequentially receives, and is transmitted to computer, and computer successively obtains the absorption of n sample cell according to chronological order, wherein
The trap of i-th of sample cell is Axi(λ), i=1 ..., the natural number that n, n are >=2；
D) modifying factor of n sample cell is calculated, the modifying factor of i-th of sample cell is ηxi,Its
Middle Axk(λ) is the trap as k-th of sample cell of verification slot；
2) multiple cuvettes for filling different test samples are inserted into respectively in corresponding sample cell；
3) by control circuit, n light source of control is successively flashed according to chronological order；
4) after the white light that light source issues is through the test sample in cuvette, the convergence of information light planoconvex lens convergence is made
With, by n unification fiber optic splitter light inlet, light-emitting window is transmitted to by fiber optic bundle；
5) diverging light that fiber optic bundle issues becomes less parallel light after the convex lens of light-emitting window, is finally covered on linear
Linear filtering on piece on the photosensitive surface of CCD element；
6) information light is sequentially received by linear CCD element according to chronological order, and transmit through after linear filtering piece
To computer, computer successively obtains the trap of the test sample in each sample cell according to chronological order, and i-th
The trap of sample cell is axi(λ), to trap axi(λ) is modified, and obtains revised trap, i-th sample cell
Revised trapTrap as final test sample.
The present invention is the characteristic of n light source, service life, strong light using the scheme greatest drawback that n light source timesharing is lighted
Degree and extinction efficiency etc. are different, and spectroscopic system is taken and can only receive a sample cell when light source and dark noise and (be set as verifying
Slot) light source, this result in the measurement result of (n-1) a sample cell at other be all using different light sources and verification slot
The measurement result obtained on the basis of dark noise etc., the i.e. light source of each sample slot all survey with reference to calculating in the light source to verification slot
Amount.
Advantages of the present invention:
The present invention has abandoned the optical grating construction of conventional spectrometers in structure, it is therefore intended that reduces cost of manufacture and uses line
Property filter plate replace grating so that space structure greatly simplifies, solve that grating spectrometer architecture is complicated, carries difficult problem；
Multiple light sources and multiple sample cells have been used in structure, improve the working efficiency of field measurement；In working mechanism, it is
The output characteristics of unified multiple light sources proposes the modification method of unified multiple light sources output characteristics, improves measurement result
Reliability, finally, realize low cost, easy processing, portable, efficient work the portable spectroscopic system of multichannel.
(1) spectroscopic system substitutes grating using linear filtering piece, and grating is greatly saved to needed for input optical dispersion expansion
The space wanted increases the portability of spectroscopic system compared with grating spectrograph；
(2) spectroscopic system uses timesharing and lights technology, allows multiple light sources successively to flash in sample cell, in time several seconds
Complete measurement, compared with grating spectrograph, increase spectroscopic system measuring speed and test sample quantity measured every time,
Substantially increase the working efficiency of spectroscopic system；
(3) spectroscopic system unifies fiber optic splitter using n, be transferred to the information light of the test sample in multiple channels can
On one linear CCD element, compared with grating spectrograph, experimental implementation is simplified, space is further saved, increasing can take
Band property；
(4) spectroscopic system is able to use material of the common plastics as sample stage, fiber optic bundle using on the market compared with
For cheap fiber optic bundle, compared to grating spectrograph, cost of manufacture and manufacture difficulty are substantially reduced.
Detailed description of the invention
Fig. 1 is the top view of one embodiment of the multichannel portable spectroscopic system of the invention lighted based on timesharing；
Fig. 2~Fig. 9 is respectively that one embodiment of the portable spectroscopic system of the multichannel lighted based on timesharing of the invention is obtained
The detection spectrogram of eight sample cells arrived.
Specific embodiment
With reference to the accompanying drawing, by specific embodiment, the present invention is further explained.
As shown in Figure 1, the portable spectroscopic system of multichannel lighted based on timesharing of the present embodiment includes: control circuit 1, eight
A light source 2, sample stage 3, eight-in-one fiber optic splitter 4, linear filtering piece 5, linear CCD element 6 and computer 7；Wherein, sample
Platform 3 is transversely disposed with eight sample cells；It is offered on each sample cell along perpendicular to 3 transverse direction of sample stage
Diameter is the light hole of 9mm, and light hole runs through the front and rear surfaces of sample cell；Control circuit 1 connects eight light sources 2, and light source uses
White light；Each light source corresponds to a sample cell, is bonded the front surface of sample cell, the corresponding light hole of face；Eight-in-one optical fiber
Beam splitter 4 is the fiber optic bundle that multifiber is formed, and one end bonding of fiber optic bundle, as light-emitting window, rubber is wrapped in light-emitting window
Rear end one convex lens of fitting, the other end of fiber optic bundle is corresponding with sample cell to be separated into stereotyped writing, as light inlet, rubber packet
The front end for being rolled in each light inlet is bonded a convex lens, is screwed the rear table for being mounted on corresponding sample cell respectively
The light hole in face；Linear filtering piece 5 is flattened on the photosensitive surface of linear CCD element 6, and the linear CCD element 6 that will be handled well
Be fixed in front of light-emitting window at the position 5~6cm, photosensitive surface just with light-emitting window horizontal alignment so that emergent light wraps completely just
Wrap photosensitive surface；Linear CCD element 6 is connected to computer 7.
The halfwidth of each spectrum channel of linear filtering piece 5 is 4.5~9.0nm, is divided in a stepwise fashion from a left side is right
It is not dispersed with the spectrum channel that 1024 energy penetrate 380nm~700nm.Linear filtering piece 5 is led to according to spectrum from left to right
The direction that road halfwidth is gradually incremented by is flattened on the photosensitive surface of linear CCD element 6.
In the present embodiment, light source uses white light LEDs light bulb, and sample stage 3 uses plastics.
Linear CCD element is independent element, just opens CCD element before booting；Alternatively, linear CCD element connects
It acquires and believes controlling the starting of linear CCD element simultaneously by the switch of control circuit 1 in booting to the switch of control circuit 1
Number.
The detection method of the portable spectroscopic system of multichannel lighted based on timesharing of the present embodiment, comprising the following steps:
1) booting enters self-test and verification demarcation flow, and while the 1st light source starts flashing, linear CCD element is simultaneously
Start information collection work, to be modified to different sample cells:
A) all samples slot is emptied, the switch of control circuit is pressed in the portable spectroscopic system booting of multichannel；
B) k-th of sample cell in n sample cell is selected as verification slot, verification slot is arbitrarily chosen, k ∈ 1,
2 ..., 8；
C) by control circuit 1, eight light sources 2 is controlled and are successively flashed according to chronological order, the white light that light source issues
After sample cell, the convergence of information light planoconvex lens by the light inlet of eight-in-one fiber optic splitter, is transmitted to out by fiber optic bundle
Optical port, the diverging light that fiber optic bundle issues become less parallel light after the convex lens of light-emitting window, are finally covered on linear CCD element
On linear filtering piece 5 on 6 photosensitive surface, after information light penetrates linear filtering piece 5, by linear CCD element 6 according to time elder generation
Sequence sequentially receives afterwards, and is transmitted to computer 7, and computer 7 successively obtains the suction of eight sample cells according to chronological order
It receives, wherein the trap of i-th of sample cell is Axi(λ), i=1 ..., 8.
D) modifying factor of eight sample cells is calculated, the modifying factor of i-th of sample cell is ηxi,
Wherein Axk(λ) is the trap as k-th of sample cell of verification slot；
2) 8 cuvettes for filling test sample are inserted into respectively in corresponding sample cell, and test sample is solution；
3) by control circuit 1, eight light sources 2 is controlled and are successively flashed according to chronological order, the 1st light source starts to dodge
While bright, computer starts information collection work simultaneously；
4) after the white light that light source issues is through the test sample in cuvette, information light planoconvex lens convergence, by eight-in-one
The light inlet of fiber optic splitter is transmitted to light-emitting window by fiber optic bundle；
5) diverging light that fiber optic bundle issues becomes less parallel light after the convex lens of light-emitting window, is finally covered on linear
On linear filtering piece 5 on the photosensitive surface of CCD element 6；
6) information light is sequentially received by linear CCD element 6 according to chronological order, and pass through after linear filtering piece 5
Computer 7 is transported to, computer 7 successively obtains the trap of the test sample in each sample cell according to chronological order,
The trap of i-th of sample cell is axi(λ), to trap axi(λ) is modified, and obtains revised trap, i-th of sample
The revised trap of product slotAs the trap of final test sample, thus within the time of several seconds
The spectrum picture for detecting eight samples in eight sample cells, respectively as shown in Fig. 2~9.
It is finally noted that the purpose for publicizing and implementing example is to help to further understand the present invention, but this field
Technical staff be understood that without departing from the spirit and scope of the invention and the appended claims, it is various replacement and repair
It is all possible for changing.Therefore, the present invention should not be limited to embodiment disclosure of that, and the scope of protection of present invention is to weigh
Subject to the range that sharp claim defines.
Claims (8)
1. a kind of portable spectroscopic system of multichannel lighted based on timesharing, which is characterized in that the portable spectroscopic system of multichannel
It include: control circuit, light source, sample stage, cuvette, n unification fiber optic splitter, linear filtering piece, linear CCD element and calculating
Machine；Wherein, the sample stage is transversely disposed with n sample cell；It is offered on each sample cell along perpendicular to sample
The light hole of sample platform transverse direction, light hole run through the front and rear surfaces of sample cell；The control circuit connects n light source, light source
Using white light；Each light source corresponds to a sample cell, is bonded the front surface of sample cell, the corresponding light hole of face；The n is closed
One fiber optic splitter is the fiber optic bundle that multifiber is formed, one end bonding of fiber optic bundle, as light-emitting window, in the rear end of light-emitting window
It is bonded a convex lens, the other end of fiber optic bundle is corresponding with sample cell to be separated into n strands, per one fiber optic bundle again by multifiber
Composition, in this, as light inlet；It is bonded a convex lens in the front end of each light inlet, is separately mounted to corresponding sample cell
Rear surface light hole；The linear filtering piece is flattened on the photosensitive surface of linear CCD element；It is pasted with linear filtering piece
Linear CCD element be fixed in front of light-emitting window, photosensitive surface and light-emitting window horizontal alignment, so that n unification fiber optic splitter
The lucky fully wrapped around firmly photosensitive surface of emergent light；The linear CCD element is connected to computer；N are filled the ratio of test sample
Color ware is inserted into respectively in corresponding sample cell, by control circuit, is controlled n light source and is successively flashed according to chronological order；
After the white light that light source issues is through the test sample in cuvette, information light planoconvex lens convergence, from n unification fiber optic splitter
Light inlet is transmitted to light-emitting window by fiber optic bundle；The diverging light that fiber optic bundle issues becomes after the converging action of light-emitting window convex lens
At less parallel light, the linear filtering on piece that is finally covered on the photosensitive surface of linear CCD element；Information light is through linear filter
It after wave plate, is sequentially received by linear CCD element according to chronological order, and be transmitted to computer, computer is according to time elder generation
Sequence successively obtains the spectrum picture of the test sample in each sample cell afterwards, wherein the natural number that n is >=2.
2. the portable spectroscopic system of multichannel as described in claim 1, which is characterized in that the control circuit includes power supply, opens
Pass and single-chip microcontroller；Wherein, power supply is connect by switch with single-chip microcontroller, and single-chip microcontroller provides the n pins that can export low and high level,
Each pin is correspondingly connected with a light source.
3. the portable spectroscopic system of multichannel as described in claim 1, which is characterized in that the light source uses white light LEDs light bulb.
4. the portable spectroscopic system of multichannel as described in claim 1, which is characterized in that the control circuit and n light source are set
It sets on circuit boards.
5. the portable spectroscopic system of multichannel as described in claim 1, which is characterized in that the sample stage is using easily molded, no
Reflect the light-proof material of specific band coloured light.
6. the portable spectroscopic system of multichannel as claimed in claim 5, which is characterized in that the sample stage is closed using plastics, aluminium
Gold or timber.
7. the detection method for the portable spectroscopic system of multichannel that one kind is lighted based on timesharing as described in claim 1, feature
It is, described detection method includes the following steps:
1) booting enters self-test and verification demarcation flow, is modified to different sample cells, obtains the modifying factor of n sample cell
Son, the modifying factor of i-th of sample cell are ηxi, i=1 ... ..., n, the natural number that n is >=2；
2) multiple cuvettes for filling different test samples are inserted into respectively in corresponding sample cell；
3) by control circuit, n light source of control is successively flashed according to chronological order；
4) after the white light that light source issues is through the test sample in cuvette, the convergence effect of information light planoconvex lens convergence, by n
The light inlet for unifying fiber optic splitter, is transmitted to light-emitting window by fiber optic bundle；
5) diverging light that fiber optic bundle issues becomes less parallel light after the convex lens of light-emitting window, is finally covered on linear CCD member
Linear filtering on piece on the photosensitive surface of part；
6) information light is sequentially received by linear CCD element according to chronological order, and be transmitted to meter through after linear filtering piece
Calculation machine, computer successively obtain the trap of the test sample in each sample cell, i-th of sample according to chronological order
The trap of slot is axi(λ), to trap axi(λ) is modified, and obtains revised trap, the amendment of i-th of sample cell
Trap afterwardsTrap as final test sample.
8. detection method as claimed in claim 7, which is characterized in that in step 1), booting enters self-test and verification calibration
Process is modified different sample cells, comprising the following steps:
A) all samples slot, the portable spectroscopic system booting of multichannel are emptied；
B) k-th of sample cell in n sample cell is selected as verification slot, and verification slot is arbitrarily chosen, k ∈ 1,2 ... ..., n；
C) by control circuit, n light source of control is successively flashed according to chronological order, and the white light that light source issues passes through sample
After slot, information light is converged through light inlet convex lens, by the light inlet of n unification fiber optic splitter, is transmitted to out light by fiber optic bundle
Mouthful, the diverging light that fiber optic bundle issues becomes less parallel light after light-emitting window convex lens, is finally covered on the sense of linear CCD element
Linear filtering on piece on optical surface, it is suitable according to chronological order by linear CCD element after information light penetrates linear filtering piece
Secondary reception, and it is transmitted to computer, computer successively obtains the absorption of n sample cell according to chronological order, wherein i-th
The trap of sample cell is Axi(λ)；
D) modifying factor of n sample cell is calculated, the modifying factor of i-th of sample cell is ηxi,Wherein Axk
(λ) is the trap as k-th of sample cell of verification slot.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110208254A (en) * | 2019-07-08 | 2019-09-06 | 中国科学技术大学 | The measuring system and method for more luminescent sample luminous intensities |
| CN112558230A (en) * | 2020-12-07 | 2021-03-26 | 中国科学院合肥物质科学研究院 | Multichannel time-sharing retest optical coupling device and use method thereof |
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| CN206818598U (en) * | 2017-05-26 | 2017-12-29 | 北京智云达科技股份有限公司 | A kind of multichannel multi-wavelength light spectrum detection device |
| CN209745804U (en) * | 2019-03-29 | 2019-12-06 | 中央民族大学 | Multi-channel portable spectrum system based on time-sharing lighting |
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| US5427920A (en) * | 1992-04-24 | 1995-06-27 | Becton Dickinson And Company | Methods and apparatus for detecting biological activities in a specimen |
| CN205808925U (en) * | 2016-06-23 | 2016-12-14 | 青岛市光电工程技术研究院 | A kind of near-infrared crude oil water content detection device |
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| CN209745804U (en) * | 2019-03-29 | 2019-12-06 | 中央民族大学 | Multi-channel portable spectrum system based on time-sharing lighting |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110208254A (en) * | 2019-07-08 | 2019-09-06 | 中国科学技术大学 | The measuring system and method for more luminescent sample luminous intensities |
| CN112558230A (en) * | 2020-12-07 | 2021-03-26 | 中国科学院合肥物质科学研究院 | Multichannel time-sharing retest optical coupling device and use method thereof |
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