CN109085148A - A kind of multichannel fluorescence detection optical system - Google Patents
A kind of multichannel fluorescence detection optical system Download PDFInfo
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- CN109085148A CN109085148A CN201811182584.3A CN201811182584A CN109085148A CN 109085148 A CN109085148 A CN 109085148A CN 201811182584 A CN201811182584 A CN 201811182584A CN 109085148 A CN109085148 A CN 109085148A
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- optical
- light
- detection
- dichroic
- splitting piece
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
Abstract
The present invention relates to a kind of multichannel fluorescence detection optical system, which includes motor, rotary shaft and optical detection apparatus；The output end of motor is fixedly connected with optical detection apparatus by rotary shaft, and optical detection apparatus is provided with two or more optical detecting channels along axisymmetry；Each optical detecting channel includes LED excitation light source, exciting light optical filter, transmitting light optical filter, dichroic light splitting piece, lens and photodiode, the monochromatic light that LED excitation light source issues is emitted to dichroic light splitting piece through exciting light optical filter, the light reflected through dichroic light splitting piece is through lens focus sample to be tested, the fluorescence that sample to be tested is generated through excitation is emitted to dichroic light splitting piece after lens are collected, and the emitted smooth optical filter of the fluorescence transmitted through dichroic light splitting piece is emitted to photodiode.Optical detecting channel of the invention carries out fluorescence signal detection using photodiode, and cost is relatively low, compact-sized, can be applied to miniature QPCR instrument and miniature Protein Detection instrument.
Description
Technical field
The present invention relates to a kind of fluorescence detecting systems, are used for bio-instruments genetic test and albumen especially with regard to one kind
The multichannel fluorescence detection optical system of quality detection.
Background technique
Real-time fluorescence quantitative PCR instrument is the important tool of current life science.It is in pathogen detection, transgenosis
Food inspection, curative effect of medication research, gene mutation analysis etc. are widely applied.Compared with traditional PCR instrument, in real time
Fluorescence quantitative PCR instrument increases optical excitation and detection system.DNA or RNA segment fluorescence signal in amplification procedure increases therewith
By force, the content of primary sample can be calculated by detecting the fluorescence signal intensity of amplification procedure.
QPCR product optical system currently on the market generally all includes excitation light source, excitation light path, exciter filter, inspection
Optical path is surveyed, optical filter, the parts such as electrooptical device are emitted.Wherein, light source part much uses White LED or tungsten halogen lamp wide
Spectroscopic light source, although a light source can excite all channels, its spectrum utilization factor is very low, and most of light energy is sent out
It penetrates, these light can be used as background interference, influence the acquisition of final signal.And the optical module volume of major part QPCR product
Greatly, structure is complicated, expensive, is only applicable to laboratory operation, product is not portable and number of channels is few, and sweep time is long.
Summary of the invention
In view of the above-mentioned problems, the simple and lower-cost multichannel fluorescence detection the object of the present invention is to provide a kind of structure
Optical system.
To achieve the above object, the present invention takes following technical scheme: a kind of multichannel fluorescence detection optical system, special
Sign is that the system includes motor, rotary shaft and optical detection apparatus；
The output end of the motor is fixedly connected with the optical detection apparatus, the optical detection dress by the rotary shaft
It sets and is provided with two or more optical detecting channels along the axisymmetry；
Each optical detecting channel include LED excitation light source, exciting light optical filter, transmitting light optical filter, two to
Color light splitting piece, lens and photodiode, the monochromatic light that the LED excitation light source issues are emitted to through the exciting light optical filter
The dichroic light splitting piece, the light through dichroic light splitting piece reflection is through the lens focus sample to be tested, sample to be tested warp
The fluorescence generated is excited to be emitted to the dichroic light splitting piece after lens collection, through dichroic light splitting piece transmission
Fluorescence is emitted to the photodiode through the transmitting light optical filter.
Further, the optical detection apparatus further includes having a shell, and the cover top portion is provided centrally with for inserting
If the through-hole of the rotary shaft, the rotary shaft pass through the through-hole fixed with the outer casing bottom, the outer casing bottom along
Axisymmetry offers detection hole corresponding with the number of optical detection channels.
Further, the wavelength of the LED light source of each optical detecting channel is all different, according to the glimmering of test agent
Signal object is determined.
Further, the quantity of the optical detecting channel is set as four.
Further, the motor uses stepper motor.
The invention adopts the above technical scheme, which has the following advantages: 1, the present invention includes motor, rotary shaft and light
Detection device is learned, the output end of motor is fixedly connected with optical detection apparatus by rotary shaft, and optical detection apparatus is along rotary shaft pair
The optical detecting channel for being provided with two or more is claimed to can detecte a variety of since the present invention is provided with multiple optical detecting channels
Fluorescent material, and each optical detecting channel is all made of LED light source, effectively increases spectrum utilization factor.2, existing detector
CCD or PMT is mostly used, volume is larger, is difficult to accomplish compact-sized, of the invention optical detecting channel using photodiode
Fluorescence signal detection is carried out, cost is relatively low, and it is compact-sized, it can be applied to miniature QPCR instrument and miniature Protein Detection instrument.
Detailed description of the invention
Fig. 1 is multichannel fluorescence detection optical system structure schematic diagram of the invention；
Fig. 2 is optical detecting channel A~D schematic diagram of optical detection apparatus of the invention, and figure (a) is that stepper motor is initial
Optical detecting channel A~D position view, figure (b) be stepper motor be rotated by 90 ° the position optical detecting channel A~D signal
Figure, figure (c) are optical detecting channel A~D position views that stepper motor rotates 180 °, and figure (d) is stepper motor rotation
270 ° of optical detecting channel A~D position view.
Specific embodiment
Come to carry out detailed description to the present invention below in conjunction with attached drawing.It should be appreciated, however, that attached drawing has been provided only more
Understand the present invention well, they should not be interpreted as limitation of the present invention.
As shown in Figure 1 and Figure 2, multichannel fluorescence detection optical system provided by the invention, including stepper motor 1, rotary shaft
2 and optical detection apparatus 3, the output end of stepper motor 1 is fixedly connected with optical detection apparatus 3 by rotary shaft 2, wherein optics
Detection device 3 is symmetrically arranged with more than two optical detecting channels along rotary shaft 2, and there are four optical detections for the present embodiment setting
Channel A~D, it is without being limited thereto as example.
Each optical detecting channel 3 include LED excitation light source 31, exciting light optical filter 32, dichroic light splitting piece 33,
Lens 34, transmitting light optical filter 35 and photodiode 36.In order to improve spectrum utilization factor, LED excitation light source 31 is using monochromatic
Light carries out fluorescence excitation, and the monochromatic light that LED excitation light source 31 issues is emitted to dichroic light splitting piece after exciting light optical filter 32
33, the light reflected through dichroic light splitting piece 33 through 34 focus detection sample of lens, fluorescence that test sample is generated through excitation along
Dichroic light splitting piece 33 is collected through lens 34 and is emitted in original optical path return, and the fluorescence transmitted through dichroic light splitting piece 33 is emitted
Light optical filter 35 is emitted to photodiode 36；Wherein, transmitting light optical filter 35 keeps fluorescence signal logical for ending exciting light
It crosses, improves signal-to-noise ratio.
Preferably, optical detection apparatus 3 further includes having a shell 37, and 37 top center of shell is provided with for plugging rotation
The through-hole of axis, rotary shaft 2 passes through through-hole and fixes with 37 bottom of shell, there are four 37 bottom of shell is symmetrically opened up along rotary shaft 2
Detection hole, corresponding each detection hole are correspondingly provided with an optical detecting channel 3, all optical devices in optical detecting channel 3
Existing fixed device is all made of to be fixed, specific structure without limitation, as long as meeting paths condition.
Preferably, the invention also includes the moduluses for carrying out analog-to-digital conversion for the output electric signal to photodiode 37 to turn
Change circuit, analog to digital conversion circuit will obtain digital signal and be input to computer by USB interface being handled to obtain test sample
Fluorescent marker, computer is also used to control the work of stepper motor 1.
Preferably, the wavelength of the LED light source 31 of each optical detecting channel 3 is all different, specifically without limitation, Ke Yigen
It is determined according to the fluorescent marker of detection reagent.
As shown in Fig. 2, multichannel fluorescence detection optical system of the invention is in use, by four of optical detection apparatus 3
Detection hole is placed on above the container equipped with test agent, drives the rotation of optical detection apparatus 3 to complete by control stepper motor 1
The detection of test agent, 90 degree of the rotation every time of stepper motor 1, returns to original position after rotating four times, so that each test agent can
Enough complete the detection of four optical detecting channels.
The various embodiments described above are merely to illustrate the present invention, wherein the structure of each component, connection type and manufacture craft etc. are all
It can be varied, all equivalents and improvement carried out based on the technical solution of the present invention should not exclude
Except protection scope of the present invention.
Claims (5)
1. a kind of multichannel fluorescence detection optical system, which is characterized in that the system includes motor, rotary shaft and optical detection dress
It sets；
The output end of the motor is fixedly connected with the optical detection apparatus, the optical detection apparatus edge by the rotary shaft
The axisymmetry is provided with two or more optical detecting channels；
Each optical detecting channel includes LED excitation light source, exciting light optical filter, transmitting light optical filter, dichroic point
Mating plate, lens and photodiode, the monochromatic light that the LED excitation light source issues are emitted to described through the exciting light optical filter
Dichroic light splitting piece, the light through dichroic light splitting piece reflection is through the lens focus sample to be tested, and sample to be tested is through exciting
The fluorescence of generation is emitted to the dichroic light splitting piece after lens collection, the fluorescence transmitted through the dichroic light splitting piece
The photodiode is emitted to through the transmitting light optical filter.
2. multichannel fluorescence detection optical system according to claim 1, which is characterized in that the optical detection apparatus is also
It include a shell, the cover top portion is provided centrally with the through-hole for plugging the rotary shaft, and the rotary shaft passes through institute
It states through-hole to fix with the outer casing bottom, the outer casing bottom offers and the optical detecting channel number along axisymmetry
Measure corresponding detection hole.
3. multichannel fluorescence detection optical system according to claim 1 or 2, which is characterized in that each optics inspection
The wavelength for the LED light source for surveying channel is all different, and is determined according to the fluorescent marker of test agent.
4. multichannel fluorescence detection optical system according to claim 1 or 2, which is characterized in that the optical detection is logical
The quantity in road is set as four.
5. multichannel fluorescence detection optical system according to claim 1 or 2, which is characterized in that the motor is using step
Into motor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811182584.3A CN109085148A (en) | 2018-10-11 | 2018-10-11 | A kind of multichannel fluorescence detection optical system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811182584.3A CN109085148A (en) | 2018-10-11 | 2018-10-11 | A kind of multichannel fluorescence detection optical system |
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| CN109085148A true CN109085148A (en) | 2018-12-25 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683869A (en) * | 2020-12-25 | 2021-04-20 | 中国科学院苏州生物医学工程技术研究所 | Fluorescent quantitative detection method |
| WO2022052362A1 (en) * | 2020-09-11 | 2022-03-17 | 广州万孚生物技术股份有限公司 | In vitro diagnostic analysis system, optical detection apparatus, and motion disc module |
| CN115144381A (en) * | 2022-09-05 | 2022-10-04 | 鲲鹏基因（北京）科技有限责任公司 | Emission light detector of PCR instrument and PCR instrument |
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| US20090059207A1 (en) * | 2006-03-06 | 2009-03-05 | Philippe Nerin | Method and device for measuring photoluminescence, absorption and diffraction of microscopic objects in a fluid |
| CN202770771U (en) * | 2012-06-15 | 2013-03-06 | 杭州安杰思医学科技有限公司 | Fluorescence quantitative detection system based on ectopic multi-wavelength detection |
| CN103033493A (en) * | 2012-12-10 | 2013-04-10 | 厦门大学 | Quantitative PCR (polymerase chain reaction) detection system for tunable fluorescent |
| CN105675574A (en) * | 2016-03-17 | 2016-06-15 | 苏州天隆生物科技有限公司 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
| CN209167125U (en) * | 2018-10-11 | 2019-07-26 | 滨江华康（北京）生物科技有限公司 | A kind of multichannel fluorescence detection optical system |
| CN110161007A (en) * | 2019-06-03 | 2019-08-23 | 杭州安誉科技有限公司 | A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence |
-
2018
- 2018-10-11 CN CN201811182584.3A patent/CN109085148A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090059207A1 (en) * | 2006-03-06 | 2009-03-05 | Philippe Nerin | Method and device for measuring photoluminescence, absorption and diffraction of microscopic objects in a fluid |
| CN202770771U (en) * | 2012-06-15 | 2013-03-06 | 杭州安杰思医学科技有限公司 | Fluorescence quantitative detection system based on ectopic multi-wavelength detection |
| CN103033493A (en) * | 2012-12-10 | 2013-04-10 | 厦门大学 | Quantitative PCR (polymerase chain reaction) detection system for tunable fluorescent |
| CN105675574A (en) * | 2016-03-17 | 2016-06-15 | 苏州天隆生物科技有限公司 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
| CN209167125U (en) * | 2018-10-11 | 2019-07-26 | 滨江华康（北京）生物科技有限公司 | A kind of multichannel fluorescence detection optical system |
| CN110161007A (en) * | 2019-06-03 | 2019-08-23 | 杭州安誉科技有限公司 | A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022052362A1 (en) * | 2020-09-11 | 2022-03-17 | 广州万孚生物技术股份有限公司 | In vitro diagnostic analysis system, optical detection apparatus, and motion disc module |
| CN112683869A (en) * | 2020-12-25 | 2021-04-20 | 中国科学院苏州生物医学工程技术研究所 | Fluorescent quantitative detection method |
| CN112683869B (en) * | 2020-12-25 | 2023-03-14 | 中国科学院苏州生物医学工程技术研究所 | Fluorescent quantitative detection method |
| CN115144381A (en) * | 2022-09-05 | 2022-10-04 | 鲲鹏基因（北京）科技有限责任公司 | Emission light detector of PCR instrument and PCR instrument |
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