CN202770771U - Fluorescence quantitative detection system based on ectopic multi-wavelength detection - Google Patents
Fluorescence quantitative detection system based on ectopic multi-wavelength detection Download PDFInfo
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- CN202770771U CN202770771U CN 201220287923 CN201220287923U CN202770771U CN 202770771 U CN202770771 U CN 202770771U CN 201220287923 CN201220287923 CN 201220287923 CN 201220287923 U CN201220287923 U CN 201220287923U CN 202770771 U CN202770771 U CN 202770771U
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Abstract
The utility model relates to a fluorescence quantitative detection system based on ectopic multi-wavelength detection, which comprises a sample pool provided with a plurality of sample reaction tubes, a detection unit, a data processing system and a display, wherein the detection unit comprises a fluorescence exciting light path and a fluorescence receiving light path; and the fluorescence exciting light path and the fluorescence receiving light path are ectopic. The fluorescence quantitative detection system has the beneficial effects that as the fluorescence exciting light path and the fluorescence receiving light path are ectopic, an incident path and a reflecting path of exciting light which are the same are avoided, so that a low-noise detection signal is realized; and a drive mechanism drives the detection unit to carry out reciprocating motion so as to realize the effect that the same one photosensitive receiver detects and receives multi-path fluorescence.
Description
Technical field
The utility model relates to the detecting instrument of biology and medical science, relates to more specifically the light path system that a kind of real-time fluorescence quantitative gene extender detects.
Background technology
Real-time fluorescence quantitative PCR detection system principle of work is to make reactant carry out temperature cycles between specific denaturation temperature, renaturation temperature and elongating temperature, the target dna fragment is carried out millions of times amplification; Tested sample in the simultaneously exciting light of the exciting light by different wave length or the single wavelength irradiation test tube, when the tested sample in the test tube is inspired fluorescence, the fluorescence intensity signals that collects by photosensitive detector sends back computing machine again and carries out real-time data analysis behind the coherent signal treatment circuit.
The fluorescence light signal detection method of traditional fluorescence quantitative gene extender is that light source is entered in vitro from the test tube top through the refraction of top cover printing opacity after the filter system colour filter, the fluorescence signal that produces is after in vitro reflection, through the test tube top cover, enter photoreceiver by exciting light and fluorescence color separation light path system again and change electric signal into.Finish the real-time detection of sample fluorescence through this light circuit.The light transmission of test tube top cover requires high, and the light transmission of test tube cap has a strong impact on the sensitivity of pattern detection.When incident light process test tube top cover reflection and refraction occur, the incident light in same propagation path and fluorescence return path will increase detection noise, be unfavorable for the detection to hypofluorescence signal, and increase follow-up filtering difficulty.
The utility model content
For overcoming the large shortcoming of prior art detection noise, the utility model provides a kind of detection noise the little fluorescent quantitation detection system based on the dystopy multi-wavelength detection.
Based on the fluorescent quantitation detection system of dystopy multi-wavelength detection, comprise sample cell with a plurality of example reaction pipes, detect unit, data handling system and display, detect the unit and comprise fluorescence excitation light path and fluorescence reception light path; It is characterized in that: fluorescence excitation light path and fluorescence reception light path dystopy.
Further, the fluorescence excitation light path comprises light source, first lens, the first optical filter that produces exciting light and will import the first leaded light prism of sample after the deflection of exciting light light path; First lens is arranged between light source and the first optical filter;
The fluorescence reception light path comprises the second lens, the second optical filter and photoreceiver, and photoreceiver is connected with data handling system, and the fluorescence of sample reflection vertically enters the second lens, and the second optical filter is between the second lens and photoreceiver.
Further, be provided with the second leaded light prism between the second optical filter and the photoreceiver, the second leaded light prism comprises the first reflecting surface and the second reflecting surface, and two reflectings surface cooperate and will import photoreceiver from the light of the second optical filter.
Further, detect the unit and drive its reciprocating driving mechanism in the sample cell zone and is connected, driving mechanism comprises motor and guide rail, motor with detect the unit and be connected, guide rail leads and detects cell moving; The every fortune of motor moves a step, the fluorescence reception light path is aimed at a sample.
Further, all samples reaction tube is arranged in a linear, and guide rail is line slideway, and motor is linear electric motors.
Further, the example reaction pipe is arranged in the alternating temperature metal module, and the alternating temperature metal module is provided with the breach that allows exciting light incident and reflected light to penetrate, and the rear end of example reaction pipe is covered against controlled thermal.
Technical conceive of the present utility model is: detect the polychrome wavelength light that the unit controls light source sends, the polychrome wavelength light is through first lens, the first optical filter enters the first leaded light prism, the first leaded light prism shines test sample with the deflection of exciting light travel direction, the fluorescence of test sample reflection is through the second lens, the second optical filter, enter the second leaded light prism, the second leaded light prism is conducted into fluorescence in the photoreceiver, by photoreceiver light signal is carried out opto-electronic conversion, the electric signal of photoreceiver output is input in the data handling system, is shown by display at last.Motor driver control driven by motor detects the unit at the reciprocating fluorescence data of collecting simultaneously each sample of guide rail.
Compared with prior art, the beneficial effects of the utility model are 1, with fluorescence excitation light path and fluorescence reception light path dystopy, avoid the incident path of exciting light identical with reflection paths, can realize the low noise detection signal.
2, driving mechanism drives and detects the unit to-and-fro movement, realizes that same photoreceiver detects reception to multichannel fluorescence.
Description of drawings
Fig. 1 is synoptic diagram of the present utility model.
Synoptic diagram when Fig. 2 is a sample of testing agency's aligning.
Embodiment
With reference to accompanying drawing, further specify the utility model:
Fluorescent quantitation detection system based on the dystopy multi-wavelength detection, comprise sample cell 10 with a plurality of example reaction pipes, detect unit, data handling system and display, display is real-time displaying device, detects the unit and comprises fluorescence excitation light path and fluorescence reception light path; Fluorescence excitation light path and fluorescence reception light path dystopy.
The fluorescence excitation light path comprises light source 1, first lens 2, the first optical filter 3 that produces exciting light and will import the first leaded light prism 4 of sample after the deflection of exciting light light path; First lens 2 is arranged between light source 1 and the first optical filter 3; Light source 1 is light emitting diode.
The fluorescence reception light path comprises the second lens 5, the second optical filter 6 and photoreceiver 8, and photoreceiver 8 is connected with data handling system, and the fluorescence of sample reflection vertically enters the second lens 5, the second optical filters 6 between the second lens 5 and photoreceiver 8.
Be provided with the second leaded light prism 7, the second leaded light prisms 7 between the second optical filter 6 and the photoreceiver 8 and comprise the first reflecting surface and the second reflecting surface, two reflectings surface cooperate and will import photoreceiver 8 from the light of the second optical filter 6.
Detect the unit and drive its reciprocating driving mechanism in the sample cell zone and is connected, driving mechanism comprises motor and guide rail, motor with detect the unit and be connected, guide rail leads and detects cell moving; The every fortune of motor moves a step, the fluorescence reception light path is aimed at a sample.
All samples reaction tube 10 is arranged in a linear, and guide rail is line slideway, and motor is linear electric motors.
Technical conceive of the present utility model is: detect the polychrome wavelength light that the unit controls light source sends, the polychrome wavelength light is through first lens 2, first filters 3 enters the first leaded light prism 4, the first leaded light prism 4 shines test sample with the deflection of exciting light travel direction, the fluorescence of test sample reflection is through the second saturating 5 mirrors, second filters 6, enter the second leaded light prism 7, the second leaded light prism 7 is conducted into fluorescence in the photoreceiver 8, carry out opto-electronic conversion by 8 pairs of light signals of photoreceiver, the electric signal of photoreceiver 8 outputs is input in the data handling system, is shown by display at last.Motor driver control driven by motor detects the unit at the reciprocating fluorescence data of collecting simultaneously each sample of guide rail.
Compared with prior art, the beneficial effects of the utility model are: 1, with fluorescence excitation light path and fluorescence reception light path dystopy, avoid the incident path of exciting light identical with reflection paths, can realize the low noise detection signal.
2, driving mechanism drives and detects the unit to-and-fro movement, realizes that same photoreceiver detects reception to multichannel fluorescence.
The described content of this instructions embodiment only is enumerating the way of realization of utility model design; protection domain of the present utility model should not be regarded as only limiting to the concrete form that embodiment states, protection domain of the present utility model also reaches the equivalent technologies means that design can be expected according to the utility model in those skilled in the art.
Claims (6)
1. based on the fluorescent quantitation detection system of dystopy multi-wavelength detection, comprise sample cell with a plurality of example reaction pipes, detect unit, data handling system and display, detect the unit and comprise fluorescence excitation light path and fluorescence reception light path; It is characterized in that: fluorescence excitation light path and fluorescence reception light path dystopy.
2. the fluorescent quantitation detection system based on the dystopy multi-wavelength detection as claimed in claim 1 is characterized in that: the fluorescence excitation light path comprises light source, first lens, the first optical filter that produces exciting light and will import the first leaded light prism of sample after the deflection of exciting light light path; First lens is arranged between light source and the first optical filter;
The fluorescence reception light path comprises the second lens, the second optical filter and photoreceiver, and photoreceiver is connected with data handling system, and the fluorescence of sample reflection vertically enters the second lens, and the second optical filter is between the second lens and photoreceiver.
3. the fluorescent quantitation detection system based on the dystopy multi-wavelength detection as claimed in claim 2, it is characterized in that: be provided with the second leaded light prism between the second optical filter and the photoreceiver, the second leaded light prism comprises the first reflecting surface and the second reflecting surface, and two reflectings surface cooperate and will import photoreceiver from the light of the second optical filter.
4. such as the described fluorescent quantitation detection system based on the dystopy multi-wavelength detection of one of claim 1-3, it is characterized in that: detect the unit and be connected with its reciprocating driving mechanism in the sample cell zone of drive, driving mechanism comprises motor and guide rail, motor with detect the unit and be connected, the guide rail guiding detects cell moving; The every fortune of motor moves a step, the fluorescence reception light path is aimed at a sample.
5. the fluorescent quantitation detection system based on the dystopy multi-wavelength detection as claimed in claim 4, it is characterized in that: all samples reaction tube is arranged in a linear, and guide rail is line slideway, and motor is linear electric motors.
6. the fluorescent quantitation detection system based on the dystopy multi-wavelength detection as claimed in claim 5, it is characterized in that: the example reaction pipe is arranged in the alternating temperature metal module, the alternating temperature metal module is provided with the breach that allows exciting light incident and reflected light to penetrate, and the rear end of example reaction pipe is covered against controlled thermal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220287923 CN202770771U (en) | 2012-06-15 | 2012-06-15 | Fluorescence quantitative detection system based on ectopic multi-wavelength detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220287923 CN202770771U (en) | 2012-06-15 | 2012-06-15 | Fluorescence quantitative detection system based on ectopic multi-wavelength detection |
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| CN202770771U true CN202770771U (en) | 2013-03-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201220287923 Expired - Lifetime CN202770771U (en) | 2012-06-15 | 2012-06-15 | Fluorescence quantitative detection system based on ectopic multi-wavelength detection |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102706849A (en) * | 2012-06-15 | 2012-10-03 | 杭州安杰思医学科技有限公司 | Ectopic multi-wavelength detection-based fluorescence quantitative detection system |
| CN109085148A (en) * | 2018-10-11 | 2018-12-25 | 滨江华康（北京）生物科技有限公司 | A kind of multichannel fluorescence detection optical system |
-
2012
- 2012-06-15 CN CN 201220287923 patent/CN202770771U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102706849A (en) * | 2012-06-15 | 2012-10-03 | 杭州安杰思医学科技有限公司 | Ectopic multi-wavelength detection-based fluorescence quantitative detection system |
| CN109085148A (en) * | 2018-10-11 | 2018-12-25 | 滨江华康（北京）生物科技有限公司 | A kind of multichannel fluorescence detection optical system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160105 Address after: Hangzhou City, Zhejiang Province, Zhenhua Road 310030 Xihu District three Town No. 320 building two layer 1 Room 201 Patentee after: HANGZHOU ANJIESI BIOTECHNOLOGY CO., LTD. Address before: Hangzhou Zhenhua Road, Zhejiang province 310030 city of Xihu District (West Lake Science Park) No. 320 building 1-2 Patentee before: Hangzhou AGS Medical Technology Co., Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130306 |