CN102706849A - Ectopic multi-wavelength detection-based fluorescence quantitative detection system - Google Patents
Ectopic multi-wavelength detection-based fluorescence quantitative detection system Download PDFInfo
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- CN102706849A CN102706849A CN2012102020871A CN201210202087A CN102706849A CN 102706849 A CN102706849 A CN 102706849A CN 2012102020871 A CN2012102020871 A CN 2012102020871A CN 201210202087 A CN201210202087 A CN 201210202087A CN 102706849 A CN102706849 A CN 102706849A
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Abstract
The invention discloses an ectopic multi-wavelength detection-based fluorescence quantitative detection system. The system comprises a sample pool provided with a plurality of sample reaction tubes, a detection unit, a data processing system and a display, wherein the detection unit comprises a fluorescence excitation light path and a fluorescent receiving light path which are ectopic. The system has the advantages that the fluorescence excitation light path and the fluorescent receiving light path are ectopic, so that the incident path and the reflection path of exciting light are different from each other; and therefore, a low-noise detection signal can be realized. A drive mechanism drives the detection unit to move reciprocally, so that a plurality of paths of fluorescence are detected and received by the same photoreceiver.
Description
Technical field
The present invention relates to the detecting instrument of biology and medical science, relate to the light path system that a kind of real-time fluorescence quantitative gene extender detects more specifically.
Background technology
Real-time fluorescence quantitative PCR detection system principle of work is to make reactant between specific denaturation temperature, renaturation temperature and elongating temperature, carry out temperature cycles, the target dna fragment is carried out millions of times amplification; Seized sample in the excitation light irradiation test tube of exciting light through different wave length or single wavelength simultaneously; When the seized sample in the test tube is inspired fluorescence; The fluorescence intensity signals that collects through photosensitive detector sends back computing machine again and carries out the real time data analysis behind the coherent signal treatment circuit.
The fluorescence light signal detection method of traditional fluorescence quantitative gene extender is that light source is got in vitro from the test tube top through the refraction of top cover printing opacity after the filter system colour filter; The fluorescence signal that produces is after reflection in vitro; Through the test tube top cover, get into photoreceiver through exciting light and fluorescence color separation light path system again and change electric signal into.Accomplish the real-time detection of sample fluorescence through this light circuit.The light transmission of test tube top cover requires high, and the light transmission of test tube lid has a strong impact on the sensitivity of pattern detection.When incident light process test tube top cover, reflection and refraction take place, the incident light in same propagation path and fluorescence return path will increase detection noise, be unfavorable for the detection to hypofluorescence signal, and increase follow-up filtering difficulty.
Summary of the invention
For overcoming the big shortcoming of prior art detection noise, the invention provides the little fluorescent quantitation detection system of a kind of detection noise based on the detection of dystopy multi-wavelength.
Based on the fluorescent quantitation detection system that the dystopy multi-wavelength detects, comprise sample cell, detect unit, data handling system and display with a plurality of example reaction pipes, detect the unit and comprise fluorescent exciting road and fluorescence receiving light path; It is characterized in that: fluorescence excitation light path and fluorescence receiving light path dystopy.
Further, the fluorescence excitation light path comprises the light source that produces exciting light, first lens, first optical filter and with the first leaded light prism that imports sample after the deflection of exciting light light path; First lens are arranged between the light source and first optical filter;
The fluorescence receiving light path comprises second lens, second optical filter and photoreceiver, and photoreceiver is connected with data handling system, vertical second lens that get into of the fluorescence of sample reflection, and second optical filter is between second lens and photoreceiver.
Further, be provided with the second leaded light prism between second optical filter and the photoreceiver, the second leaded light prism comprises first reflecting surface and second reflecting surface, and two reflectings surface cooperate and will import photoreceiver from the light of second optical filter.
Further, detect the unit and drive its reciprocating driving mechanism in the sample cell zone and is connected, driving mechanism comprises motor and guide rail, motor with detect the unit and be connected, guide rail leads and detects cell moving; The every fortune of motor moves a step, the fluorescence receiving light path is aimed at a sample.
Further, all samples reaction tube is arranged in a linear, and guide rail is a line slideway, and motor is linear electric motors.
Further, the example reaction pipe is arranged in the alternating temperature metal module, and the alternating temperature metal module is provided with the breach that allows exciting light incident and reflected light to penetrate, and the rear end of example reaction pipe is covered against controlled thermal.
Technical conceive of the present invention is: detect the polychrome wavelength light that the unit controls light source sends, the polychrome wavelength light is through first lens, and first optical filter gets into the first leaded light prism; The first leaded light prism shines test sample with the deflection of exciting light travel direction; The fluorescence of test sample reflection is through second lens, and second optical filter gets into the second leaded light prism; The second leaded light prism is conducted into fluorescence in the photoreceiver; By photoreceiver light signal is carried out opto-electronic conversion, the electric signal of photoreceiver output is input in the data handling system, is shown by display at last.Motor driver control driven by motor detects the unit at the reciprocating fluorescence data of collecting each sample simultaneously of guide rail.
Compared with prior art, the invention has the beneficial effects as follows 1,, avoid the incident path of exciting light identical, can realize the low noise detection signal with reflection paths with fluorescence excitation light path and fluorescence receiving light path dystopy.
2, driving mechanism drives and detects the unit to-and-fro movement, realizes that same photoreceiver detects reception to multichannel fluorescence.
Description of drawings
Fig. 1 is a synoptic diagram of the present invention.
Synoptic diagram when Fig. 2 is sample of testing agency's aligning.
Embodiment
With reference to accompanying drawing, further specify the present invention:
Fluorescent quantitation detection system based on the detection of dystopy multi-wavelength; Comprise sample cell 10, detect unit, data handling system and display with a plurality of example reaction pipes; Display is a real-time displaying device, detects the unit and comprises fluorescent exciting road and fluorescence receiving light path; Fluorescence excitation light path and fluorescence receiving light path dystopy.
The fluorescence excitation light path comprises the light source 1 that produces exciting light, first lens 2, first optical filter 3 and with the first leaded light prism 4 that imports sample after the deflection of exciting light light path; First lens 2 are arranged between the light source 1 and first optical filter 3; Light source 1 is a light emitting diode.
The fluorescence receiving light path comprises second lens 5, second optical filter 6 and photoreceiver 8, and photoreceiver 8 is connected with data handling system, and vertical second lens, 5, the second optical filters 6 that get into of the fluorescence of sample reflection are between second lens 5 and photoreceiver 8.
Be provided with the second leaded light prism, 7, the second leaded light prisms 7 between second optical filter 6 and the photoreceiver 8 and comprise first reflecting surface and second reflecting surface, two reflectings surface cooperate and will import photoreceiver 8 from the light of second optical filter 6.
Detect the unit and drive its reciprocating driving mechanism in the sample cell zone and is connected, driving mechanism comprises motor and guide rail, motor with detect the unit and be connected, guide rail leads and detects cell moving; The every fortune of motor moves a step, the fluorescence receiving light path is aimed at a sample.
All samples reaction tube 10 is arranged in a linear, and guide rail is a line slideway, and motor is linear electric motors.
Technical conceive of the present invention is: detect the polychrome wavelength light that the unit controls light source sends, the polychrome wavelength light filters 3 through first lens 2, the first and gets into the first leaded light prism 4; The first leaded light prism 4 shines test sample with the deflection of exciting light travel direction; The fluorescence warp second of test sample reflection passes through 5 mirrors, and second filters 6, gets into the second leaded light prism 7; The second leaded light prism 7 is conducted into fluorescence in the photoreceiver 8; Carry out opto-electronic conversion by 8 pairs of light signals of photoreceiver, the electric signal of photoreceiver 8 outputs is input in the data handling system, is shown by display at last.Motor driver control driven by motor detects the unit at the reciprocating fluorescence data of collecting each sample simultaneously of guide rail.
Compared with prior art, the invention has the beneficial effects as follows: 1,, avoid the incident path of exciting light identical, can realize the low noise detection signal with reflection paths with fluorescence excitation light path and fluorescence receiving light path dystopy.
2, driving mechanism drives and detects the unit to-and-fro movement, realizes that same photoreceiver detects reception to multichannel fluorescence.
The described content of this instructions embodiment only is enumerating the way of realization of inventive concept; Protection scope of the present invention should not be regarded as and only limit to the concrete form that embodiment states, protection scope of the present invention also reach in those skilled in the art conceive according to the present invention the equivalent technologies means that can expect.
Claims (6)
1. the fluorescent quantitation detection system that detects based on the dystopy multi-wavelength comprises sample cell with a plurality of example reaction pipes, detects unit, data handling system and display, detects the unit and comprises fluorescent exciting road and fluorescence receiving light path; It is characterized in that: fluorescence excitation light path and fluorescence receiving light path dystopy.
2. the fluorescent quantitation detection system that detects based on the dystopy multi-wavelength as claimed in claim 1 is characterized in that: the fluorescence excitation light path comprises the light source that produces exciting light, first lens, first optical filter and with the first leaded light prism of importing sample after the deflection of exciting light light path; First lens are arranged between the light source and first optical filter;
The fluorescence receiving light path comprises second lens, second optical filter and photoreceiver, and photoreceiver is connected with data handling system, vertical second lens that get into of the fluorescence of sample reflection, and second optical filter is between second lens and photoreceiver.
3. the fluorescent quantitation detection system that detects based on the dystopy multi-wavelength as claimed in claim 2; It is characterized in that: be provided with the second leaded light prism between second optical filter and the photoreceiver; The second leaded light prism comprises first reflecting surface and second reflecting surface, and two reflectings surface cooperate and will import photoreceiver from the light of second optical filter.
4. like the described fluorescent quantitation detection system that detects based on the dystopy multi-wavelength of one of claim 1-3; It is characterized in that: detect the unit and be connected with its reciprocating driving mechanism in the sample cell zone of drive; Driving mechanism comprises motor and guide rail; Motor with detect the unit and be connected, the guide rail guiding detects cell moving; The every fortune of motor moves a step, the fluorescence receiving light path is aimed at a sample.
5. the fluorescent quantitation detection system that detects based on the dystopy multi-wavelength as claimed in claim 4, it is characterized in that: all samples reaction tube is arranged in a linear, and guide rail is a line slideway, and motor is linear electric motors.
6. the fluorescent quantitation detection system that detects based on the dystopy multi-wavelength as claimed in claim 5; It is characterized in that: the example reaction pipe is arranged in the alternating temperature metal module; The alternating temperature metal module is provided with the breach that allows exciting light incident and reflected light to penetrate, and the rear end of example reaction pipe is covered against controlled thermal.
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| CN2012102020871A CN102706849A (en) | 2012-06-15 | 2012-06-15 | Ectopic multi-wavelength detection-based fluorescence quantitative detection system |
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| CN2012102020871A CN102706849A (en) | 2012-06-15 | 2012-06-15 | Ectopic multi-wavelength detection-based fluorescence quantitative detection system |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063642A (en) * | 2012-12-26 | 2013-04-24 | 西北农林科技大学 | Portable multi-index fluorescent biosensor device |
| CN103969229A (en) * | 2013-01-30 | 2014-08-06 | 全视科技有限公司 | Fluorescence imaging module |
| CN109060672A (en) * | 2018-08-22 | 2018-12-21 | 广州万孚生物技术股份有限公司 | Systems for optical inspection |
| CN110726704A (en) * | 2019-10-11 | 2020-01-24 | 北京茂泽科技有限公司 | Full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer |
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| WO2009051416A1 (en) * | 2007-10-16 | 2009-04-23 | Seed Biochips Co., Ltd. | Portable optical device for multi-chamber fluorescence measurement and method for multi-chamber fluorescence measurement using the same |
| CN101699271A (en) * | 2009-10-27 | 2010-04-28 | 西安天隆科技有限公司 | Real-time fluorescence quantitative PCR excitation detection system |
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| CN202770771U (en) * | 2012-06-15 | 2013-03-06 | 杭州安杰思医学科技有限公司 | Fluorescence quantitative detection system based on ectopic multi-wavelength detection |
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| CN101021477A (en) * | 2007-03-07 | 2007-08-22 | 博奥生物有限公司 | Hot air circulating temperature controlling real-time fluorescent quantitative PCR detector |
| WO2009051416A1 (en) * | 2007-10-16 | 2009-04-23 | Seed Biochips Co., Ltd. | Portable optical device for multi-chamber fluorescence measurement and method for multi-chamber fluorescence measurement using the same |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063642A (en) * | 2012-12-26 | 2013-04-24 | 西北农林科技大学 | Portable multi-index fluorescent biosensor device |
| CN103969229A (en) * | 2013-01-30 | 2014-08-06 | 全视科技有限公司 | Fluorescence imaging module |
| CN103969229B (en) * | 2013-01-30 | 2016-12-07 | 豪威科技股份有限公司 | Fluorescence imaging module |
| CN109060672A (en) * | 2018-08-22 | 2018-12-21 | 广州万孚生物技术股份有限公司 | Systems for optical inspection |
| CN110726704A (en) * | 2019-10-11 | 2020-01-24 | 北京茂泽科技有限公司 | Full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer |
| CN110726704B (en) * | 2019-10-11 | 2024-04-12 | 北京茂泽科技有限公司 | Full-automatic multi-wavelength angle excitation fluorescence photometry detection analyzer |
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Application publication date: 20121003 |