CN101158644A - Rotary type multichannel inducing fluorescent apparatus and method based on transmit optical fiber - Google Patents
Rotary type multichannel inducing fluorescent apparatus and method based on transmit optical fiber Download PDFInfo
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- CN101158644A CN101158644A CNA200710177464XA CN200710177464A CN101158644A CN 101158644 A CN101158644 A CN 101158644A CN A200710177464X A CNA200710177464X A CN A200710177464XA CN 200710177464 A CN200710177464 A CN 200710177464A CN 101158644 A CN101158644 A CN 101158644A
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Abstract
The invention relates to a device and belongs to the photoelectric detection field. The device sequentially comprises a light source (1), an incidence coupling lens (3), a dichroic mirror (6), transmission optical fibre (4) and an outlet coupling lens (7), and is characterized in that the transmission optical fibre (4) is a group of multichannel transmission optical fibre; the rear of the outlet coupling lens (7) comprises a releasing optical filter or a prism device (8) and a photoelectric detector (9) connected with a computer (10); The light of the light source (1) split by the dichroic mirror enters the transmission optical fibre; The fluorescence emitted by the transmission optical fibre is split by the dichroic mirror and becomes parallel light through the outlet coupling lens, and the fluorescence filtered or split by the releasing optical filter or the prism device is projected on a rotatable disk to drive a motor for driving the disk to rotate; a hole is opened in the position where the fluorescence is projected on the disk, after passing through the hole, the projection light is vertically injected into the photoelectric detector to complete a channel detection; the rotation of the disk realizes a multichannel detection. The mechatronics of the invention has simple structure, fast speed and high detection precision.
Description
Technical field
The present invention is a kind of rotary type multichannel inducing fluorescent apparatus and method based on Transmission Fibers, belongs to the photoelectric detection technology field in the biomedicine.
Background technology
Because external light source excites object to be detected to derive the detection mode of fluorescence, very little for influence biological, animal and human's body, the more important thing is the dynamic range of fluoroscopic examination and sensitivity more and more higher near or catch up with radiological measuring, therefore fluoroscopic examination is widely used among life science, medical research and the practical application, especially the real-time detection range of live body of biology, animal and human's body etc.
Classical hyperchannel excites inducing fluorescent apparatus and method, utilize an input optical fibre and another root output optical fibre to come transmission ray, promptly utilize an input optical fibre, light transmission irradiation reaction tank with light source, excite biological sample to derive fluorescence, to derive fluorescence again and project photodetector, detect by another root output optical fibre output.For two-dimensional matrix formula multi-channel detection, in one plane scan each passage and finish multi-channel detection along X and the Y direction mobile input optical fibre of two dimension and output optical fibre.
In the said apparatus, utilize the pliability moving fiber of optical fiber to detect, for two-dimensional matrix formula multi-channel detection, moving fiber carries out X and Y two dimensional motion, so speed is slow, location and repetitive positioning accuracy are poor.Speed is slow, causes each pathway reaction to have difference in time; Bearing accuracy is poor, and the intensity of illumination of each passage has difference, causes out of true between each channel measurement; Repetitive positioning accuracy is poor, causes the duplicate measurements precision of single passage that difference is arranged.
Summary of the invention
The object of the present invention is to provide a kind of rotary type multichannel inducing fluorescent apparatus based on Transmission Fibers, improve existing optical system, promptly utilize one group of multi-channel optical fibre external excitation light to be introduced the irradiation sample, utilize another group multi-channel optical fibre to do the fluorescence that the output light path transmission derives simultaneously again as input channel.Drive the rotation of driven by motor disk, allow transmission light in the output optical fibre of every road pass through perforate on the disk successively, project photodetector, realize multi-channel detection by rotating circular disk.Two dimensional surface with X and Y direction moves by the replacement that rotatablely moves like this, avoided the two dimension of optical system to move, this device has simple, the fireballing advantage of electromechanical structure, overcomes a location and a repetitive positioning accuracy difference difficult problem simultaneously, has the high advantage of accuracy of detection.
A kind of rotary type multichannel inducing fluorescent apparatus based on Transmission Fibers comprises light source, incident coupled lens, dichroic mirror, Transmission Fibers, Transmission Fibers, dichroic mirror, output coupled lens successively, it is characterized in that:
Transmission Fibers is one group of multi-channel optical fibre, not only does input but also do output light path; The fluorescence that described Transmission Fibers is come out becomes directional light by the output coupled lens, all project a rotatable disk through the arrowband fluorescence that discharges light filter (Emission Filter) or light-dividing device filtering or beam split, drive motor drives the disk rotation; Project the disk place at fluorescence and open a hole, projection light impinges perpendicularly on photodetector by behind this hole, and photodetector connects computing machine, finishes the detection of a passage; The driver plate rotation is surveyed the fluorescence that transmits in the output optical fibre of every road successively by perforate (passage gating), realize multi-channel detection.
Between light source, input coupled lens, excitation light filter (Excitation Filter) or optical splitter can also be set, the light source that generally is used for LED and the wide spectrum of Halogen lamp LED, light filtering or beam split that light source is sent become monochromatic light, have the little advantage of the ground unrest of detection.
Use the method for above-mentioned rotary type multichannel inducing fluorescent apparatus based on Transmission Fibers, may further comprise the steps:
1, opens light source 1 preheating, up to luminous stable;
2, the light that sends of light source 1 is assembled through incident coupled lens 3, through dichroic mirror 6 beam split, is coupled into one group of multipath transmission optical fiber 4, from Transmission Fibers 4 sample 5 the back irradiation reaction tank that comes out, and excited sample 5 is sent fluorescence;
3, the fluorescence that comes out of excited sample is coupled in one group of multipath transmission optical fiber 4, the fluorescence that comes out from Transmission Fibers 4, through dichroic mirror 6 beam split, become directional light by output coupled lens 7, all project a rotatable disk through the arrowband fluorescence that discharges light filter or light-dividing device 8 filtering or beam split, drive motor drives the disk rotation; Project the disk place at fluorescence and open a hole, projection light impinges perpendicularly on photodetector 9 by behind this hole, and photodetector 9 carries out opto-electronic conversion, amplification, filtering and analog-to-digital conversion, digital signal after the conversion enters and shows in the computing machine 10 and processing, finishes the detection of a passage; The driver plate rotation is surveyed the fluorescence that transmits in the Transmission Fibers of every road successively by perforate (passage gating), realize multi-channel detection.
The invention has the advantages that: drive motor drives the disk rotation, makes the fluorescence that transmits in the Transmission Fibers of every road successively by the perforate on the disk, projects photodetector, realizes multi-channel detection.Two dimensional surface with X and Y direction moves by the replacement that rotatablely moves like this, and this device has simple, the fireballing advantage of electromechanical structure, overcomes a location and a repetitive positioning accuracy difference difficult problem simultaneously, has the high advantage of accuracy of detection.
Description of drawings
Fig. 1 is a single channel pick-up unit structure principle chart of the present invention;
Fig. 2 is the synoptic diagram that the present invention is based on the rotary 96 passage inducing fluorescent apparatus of Transmission Fibers;
Embodiment:
Below in conjunction with accompanying drawing, the present invention will be described in detail.
Embodiment:
For single channel pick-up unit as shown in Figure 1, the light that light source 1 sends becomes monochromatic light through excitation light filter 2, assemble by incident coupled lens 3, through dichroic mirror 6 beam split, be coupled in the optical fiber of 4 li of one group of multipath transmission optical fiber, monochromatic light is from Transmission Fibers 4 sample 5 the back irradiation reaction tank that comes out, excited sample 5 is sent fluorescence; The fluorescence that ejects is coupled in the optical fiber of 4 li of one group of multipath transmission optical fiber, the fluorescence that comes out from Transmission Fibers 4 passes through dichroic mirror 6 beam split, become directional light by output coupled lens 7, all project a rotatable disk through the arrowband fluorescence that discharges 8 filtering of light filter, drive motor drives the disk rotation; Project the disk place at fluorescence and open a hole, projection fluorescence impinges perpendicularly on photodetector 9 by behind this hole, and photodetector 9 carries out opto-electronic conversion, amplification, filtering and analog-to-digital conversion, digital signal after the conversion enters and shows in the computing machine 10 and processing, finishes the detection of a passage.
For rotary 96 passage inducing fluorescent apparatus as shown in Figure 2 based on Transmission Fibers, the light that light source 1 sends, become monochromatic light by excitation light filter 2, assemble through incident coupled lens 3 again, by dichroic mirror 6 beam split, after being coupled into one group of multipath transmission optical fiber 4, monochromatic light and coming out from Transmission Fibers 4, sample 5 in the irradiation reaction tank, excited sample 5 is sent fluorescence; The fluorescence that sends directly is coupled in the Transmission Fibers 4, the fluorescence that comes out from Transmission Fibers 4, through dichroic mirror 6 beam split, become directional light by output coupled lens 7 again, all project a rotatable disk through the arrowband fluorescence that discharges 8 filtering of light filter, drive motor drives the disk rotation; Open a hole to the disk place in ray cast, projection fluorescence impinges perpendicularly on photodetector 9 by behind this hole, and photodetector 9 carries out opto-electronic conversion, amplification, filtering and analog-to-digital conversion, digital signal after the conversion enters and shows in the computing machine 10 and processing, finishes the detection of a passage; Driver plate rotation allows the transmission light in the Transmission Fibers of every road survey realization multi-channel detection (for some devices among the figure that gives top priority to what is the most important are not indicated) successively by perforate (passage gating).
Incident coupled lens 3 is for being provided with lens before every road Transmission Fibers 4, and lens can be common dialyte lenses; Lens can be to be produced on input optical fibre 4 end faces of every road.
Output coupled lens 7 can be for being provided with lens before or the every road Transmission Fibers 4.When being provided with an output coupled lens, every road Transmission Fibers 4 is placed on the front focal plane of these lens.When being provided with an output coupled lens before every road Transmission Fibers 4, every road Transmission Fibers 4 is placed on the front focal plane of each output coupled lens, and lens can be common dialyte lenses; Lens can be to be produced on Transmission Fibers 4 front focal planes of every road.
Utilize the two dimensional surface motion of X and Y direction in general, one time 96 hole fluoroscopic examination time is in 3 seconds, and by a two dimensional surface motion that rotatablely moves and replace X and Y direction, prior art is very easy to the driven by motor disk motion of 1000 revolutions per seconds of control rotating speeds, and one time 96 hole fluoroscopic examination time is in 0.001 second.
Claims (6)
1. the rotary type multichannel inducing fluorescent apparatus based on Transmission Fibers comprises light source (1), incident-coupled lens (3), dichroic mirror (6), Transmission Fibers (4), output coupled lens (7) successively, it is characterized in that:
Transmission Fibers (4) is one group of multipath transmission optical fiber, not only does input but also do output light path; Output coupled lens (7) also comprise successively afterwards discharge light filter or light-dividing device (8), photodetector (9) connects computing machine (10);
Beam split enters Transmission Fibers (4) to the light of light source (1) through dichroic mirror (6);
The fluorescence that described Transmission Fibers (4) is come out is through dichroic mirror (6) beam split, become directional light by output coupled lens (7), all project a rotatable disk through the fluorescence that discharges light filter or light-dividing device (8) filtering or beam split, drive motor drives the disk rotation; Project the disk place at fluorescence and open a hole, projection light impinges perpendicularly on photodetector (9) by behind this hole, and photodetector (9) connects computing machine (10), finishes the detection of a passage; Driver plate rotation allows the transmission light in the output optical fibre of every road survey by perforate successively, realizes multi-channel detection.
2. the rotary type multichannel inducing fluorescent apparatus based on Transmission Fibers according to claim 1 is characterized in that: excitation light filter or optical splitter (2) can also be set between light source (1), incident coupled lens (3).
3. the rotary type multichannel inducing fluorescent apparatus based on Transmission Fibers according to claim 1 is characterized in that: described incident coupled lens (3) every road Transmission Fibers (4) is provided with one before.
4. the rotary type multichannel inducing fluorescent apparatus based on Transmission Fibers according to claim 1 is characterized in that: dichroic mirror (6) beam split is set between incident coupled lens (3), Transmission Fibers (4).
5. the rotary type multichannel inducing fluorescent apparatus based on Transmission Fibers according to claim 1 is characterized in that: described output coupled lens (7) is that one or every road Transmission Fibers (4) are provided with one before.
6. application rights requires 1 described rotary type multichannel inducing fluorescent apparatus based on Transmission Fibers
Method is characterized in that, may further comprise the steps:
1) opens light source (1) preheating, up to luminous stable;
2) light that sends of light source (1) is assembled through incident coupled lens (3), through dichroic mirror (6) beam split, is coupled into one group of multipath transmission optical fiber (4), from Transmission Fibers (4) sample (5) the back irradiation reaction tank that comes out, and excited sample (5) is sent fluorescence;
3) fluorescence that comes out of excited sample is coupled in one group of multipath transmission optical fiber (4), the fluorescence that comes out from Transmission Fibers (4), through dichroic mirror (6) beam split, become directional light by output coupled lens (7), all project a rotatable disk through the arrowband fluorescence that discharges light filter or light-dividing device (8) filtering or beam split, drive motor drives the disk rotation; Project the disk place at fluorescence and open a hole, projection light is by behind this hole, impinge perpendicularly on photodetector (9), photodetector (9) carries out opto-electronic conversion, amplification, filtering and analog-to-digital conversion, digital signal after the conversion enters and shows in the computing machine (10) and processing, finishes the detection of a passage; Driver plate rotation allows the transmission light in the output optical fibre of every road survey by perforate successively, realizes multi-channel detection.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200710177464XA CN101158644B (en) | 2007-11-16 | 2007-11-16 | Rotary type multichannel fluorescentinducing method based on transmission optical fiber |
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| CN200710177464XA CN101158644B (en) | 2007-11-16 | 2007-11-16 | Rotary type multichannel fluorescentinducing method based on transmission optical fiber |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175612A (en) * | 2011-01-18 | 2011-09-07 | 河北工业大学 | Multi-path photoelectric detection device |
| CN103338068A (en) * | 2013-06-28 | 2013-10-02 | 华中科技大学 | Light splitting monitoring device based on multi-channel parallel optical signals |
| CN104568875A (en) * | 2014-12-22 | 2015-04-29 | 北京工业大学 | Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system |
| CN105223181A (en) * | 2015-10-26 | 2016-01-06 | 肯维捷斯（武汉）科技有限公司 | A kind of fluorescence detection device |
| CN105527263A (en) * | 2016-01-15 | 2016-04-27 | 公安部第一研究所 | Optical fiber beam splitting method and device for laser-induced fluorescent light path |
| CN105675574A (en) * | 2016-03-17 | 2016-06-15 | 苏州天隆生物科技有限公司 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
| CN106353289A (en) * | 2016-09-19 | 2017-01-25 | 苏州微析生物科技有限公司 | Light path system of POCT fluorescent quantitative analyser and fluorescent quantitative analysis method |
Family Cites Families (6)
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| JP2001091463A (en) * | 1999-09-22 | 2001-04-06 | Tosoh Corp | Scanner type fluorescence detector using small-sized exciting light source |
| CN1311436A (en) * | 2000-03-01 | 2001-09-05 | 上海和泰光电科技有限公司 | Reading of biological chip fluorescent image on rotary platform |
| CN1137400C (en) * | 2001-08-23 | 2004-02-04 | 华中科技大学 | Fast deep scanning and imaging method |
| CN1176367C (en) * | 2002-01-11 | 2004-11-17 | 清华大学 | Automatic focus regulator for laser cofocal scanner |
| CN101013136B (en) * | 2007-02-08 | 2011-07-20 | 北京工业大学 | Laser-induction fluorescence co-focusing scanning device and method |
| CN201107270Y (en) * | 2007-11-16 | 2008-08-27 | 北京工业大学 | Rotary type multichannel inducing fluorescent apparatus based on transmission optical fiber |
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2007
- 2007-11-16 CN CN200710177464XA patent/CN101158644B/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175612A (en) * | 2011-01-18 | 2011-09-07 | 河北工业大学 | Multi-path photoelectric detection device |
| CN102175612B (en) * | 2011-01-18 | 2012-12-26 | 河北工业大学 | Multi-path photoelectric detection device |
| CN103338068A (en) * | 2013-06-28 | 2013-10-02 | 华中科技大学 | Light splitting monitoring device based on multi-channel parallel optical signals |
| CN103338068B (en) * | 2013-06-28 | 2016-04-13 | 华中科技大学 | A kind of point photo detector based on multi-channel parallel light signal |
| CN104568875A (en) * | 2014-12-22 | 2015-04-29 | 北京工业大学 | Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system |
| CN104568875B (en) * | 2014-12-22 | 2017-02-22 | 北京工业大学 | Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system |
| CN105223181A (en) * | 2015-10-26 | 2016-01-06 | 肯维捷斯（武汉）科技有限公司 | A kind of fluorescence detection device |
| CN105527263A (en) * | 2016-01-15 | 2016-04-27 | 公安部第一研究所 | Optical fiber beam splitting method and device for laser-induced fluorescent light path |
| CN105675574A (en) * | 2016-03-17 | 2016-06-15 | 苏州天隆生物科技有限公司 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
| CN105675574B (en) * | 2016-03-17 | 2018-08-10 | 西安天隆科技有限公司 | More fluorescence channel detecting systems for real-time fluorescence quantitative PCR |
| CN106353289A (en) * | 2016-09-19 | 2017-01-25 | 苏州微析生物科技有限公司 | Light path system of POCT fluorescent quantitative analyser and fluorescent quantitative analysis method |
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