CN109521197B - Multi-channel fluorescence immunoassay analyzer - Google Patents
Multi-channel fluorescence immunoassay analyzer Download PDFInfo
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- CN109521197B CN109521197B CN201811643387.7A CN201811643387A CN109521197B CN 109521197 B CN109521197 B CN 109521197B CN 201811643387 A CN201811643387 A CN 201811643387A CN 109521197 B CN109521197 B CN 109521197B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
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Abstract
The invention belongs to the technical field of clinical detection medical treatment, and relates to a multi-channel fluorescence immunoassay analyzer, which comprises a reagent card placing system, a scanning detection system, a signal acquisition and conversion system and a guide rail system; the reagent card placement system is used for detachably mounting a plurality of reagent cards; the scanning detection system is used for generating emission light, irradiating the emission light on the reagent card and collecting reflected light of the reagent card; the signal acquisition and conversion system is used for detecting the fluorescence intensity of the reagent card through photoelectric signal processing; the guide rail system is used for conveying the scanning detection system along the extension direction of the reagent card placing system, so that the scanning detection system sequentially passes through the reagent cards arranged in the reagent card placing system; the invention integrates the analysis of the problems in the prior art, adopts a linear multi-channel scheme, can meet the requirement of multi-channel detection, simplifies the structure and ensures the accuracy and reliability of the detection.
Description
Technical Field
The invention belongs to the technical field of clinical detection and medical treatment, and relates to a multi-channel fluorescence immunoassay analyzer.
Background
Most of the existing fluorescence analyzers are single-channel devices; the single-channel detection equipment cannot realize simultaneous detection of multiple items of a single sample, multiple items of one sample need to be detected for multiple times, the efficiency is low, the speed is low, and when the sample size is large, the detection requirement cannot be met by the single-channel equipment.
The existing multi-channel fluorescence analyzer adopts a rotating disc type structure, and has the problems of complex structure, poor reliability, and poor accuracy and stability of measurement results. The situation that the card is failed to enter and the analyzer is stuck is easily caused; the reagent card is easy to turn over and seize, and the noise is high; when the turntable rotates, the reagent card performs centrifugal motion, so that the deviation is easy to generate, and the repeatability of measurement is influenced; when the reagent card moves, the light source also moves, and the reagent card and the light source generate relative movement, so that the accuracy and the stability of a measuring result are poor; the rotating disc can not run when the card is discharged, and the reagent card can not reach the measurement in accurate time, so that various problems of poor accuracy and stability of the measurement result and the like are caused.
Disclosure of Invention
In view of this, the present invention provides a multi-channel fluorescence immunoassay analyzer, which can satisfy the requirement for detecting a plurality of detection reagent cards and improve the processing efficiency under the condition of ensuring the stability of the optical path detection system.
In order to achieve the purpose, the invention provides the following technical scheme:
a multi-channel fluorescence immunoassay analyzer comprises a reagent card placing system, a scanning detection system, a signal acquisition and conversion system and a guide rail system; the reagent card placement system is used for detachably mounting a plurality of reagent cards; the scanning detection system is used for generating emission light, irradiating the emission light on the reagent card and collecting reflected light of the reagent card; the signal acquisition and conversion system is used for converting the reflected light signals acquired by the scanning detection system into electric signals and detecting the fluorescence intensity of the reagent card through photoelectric signal processing; the scanning detection system is arranged on the guide rail system, and the guide rail system is used for conveying the scanning detection system along the extension direction of the reagent card placing system, so that the scanning detection system sequentially passes through the reagent cards arranged in the reagent card placing system.
Optionally, the extension direction of the guide rail system is a straight line, and the guide rail system comprises belt pulleys, a conveyor belt and a first driving motor, the scanning detection system is mounted on the conveyor belt, the number of the belt pulleys is two, and the conveyor belt sequentially bypasses the two belt pulleys; the first driving motor is used for driving one belt wheel to rotate so as to drive the scanning detection system to move forwards; the extending direction of the reagent card placing system is also a straight line, and the extending direction of the reagent card placing system is parallel to the extending direction of the guide rail system.
Optionally, the first drive motor is mounted on a motor mounting plate.
Optionally, a photoelectric switch for detecting the position of the scanning detection system is further disposed on the guide rail system.
Optionally, the guide rail system further includes a guide rail for guiding a traveling direction of the scanning detection system, the scanning detection system is mounted on the guide rail, and an extending direction of the guide rail is parallel to an extending direction of the conveyor belt.
Optionally, the guide rail is further provided with a guide rail sliding block and a sliding block connecting plate fixedly connected with the guide rail sliding block, and the scanning detection system is connected with the sliding block through the sliding block connecting plate and is installed on the guide rail.
Optionally, a travel detection switch for detecting the position of the rail slider is further included.
Optionally, the scanning detection system includes a second driving motor, a photoelectric chute, a photoelectric slider, a photoelectric conversion sensor, and an excitation light source; the photoelectric sliding block is arranged in the photoelectric sliding groove and is driven by a second driving motor to move along the direction close to or far away from the reagent card; the excitation light source is arranged on the photoelectric slide block and generates emission light; the photoelectric conversion sensor is mounted on the photoelectric slider and collects reflected light.
Optionally, the photoelectric slider is further provided with an emission light filter and a reflection light filter, and emission light generated by the excitation light source is irradiated on the reagent card through the emission light filter; the reflected light from the reagent card is transmitted through the reflected light filter and is applied to the photoelectric conversion sensor.
Optionally, the optical module further comprises a scanning detection connection board, and the excitation light source, the photoelectric conversion sensor, the emission light filter and the reflection light filter are all connected with the photoelectric slider through the scanning detection connection board.
Optionally, the reagent card placing system comprises a plurality of reagent card slots arranged in parallel; each reagent clamping groove is internally provided with a spring piece for clamping the reagent card and a reagent clamping groove blocking piece for limiting the position of the reagent card in the reagent clamping groove.
Optionally, each reagent card slot is further provided with a display lamp for detecting whether a reagent card is installed in the reagent card slot.
Optionally, a plurality of parallel reagent card slots are all installed on the reagent card placing plate.
Optionally, the reagent card placing system, the scanning detection system, the signal acquisition and conversion system, and the guide rail system are all disposed on the mounting base plate.
The invention has the beneficial effects that:
the invention integrates the analysis of the problems in the prior art, adopts a linear multi-channel scheme, can meet the requirement of multi-channel detection, simplifies the structure and ensures the accuracy and reliability of the detection.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and combinations particularly pointed out hereinafter.
Drawings
For the purposes of promoting a better understanding of the objects, aspects and advantages of the invention, reference will now be made to the following detailed description taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic structural view of a rail system;
FIG. 3 is a schematic diagram of a scanning inspection system;
fig. 4 is a schematic diagram of the installation positions of the excitation light source and the photoelectric conversion sensor;
FIG. 5 is a light path diagram;
fig. 6 is a flowchart illustrating the operation of the detection process.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention. It should be noted that the drawings provided in the following embodiments are only for illustrating the basic idea of the present invention in a schematic way, and the features in the following embodiments and examples may be combined with each other without conflict.
Wherein the showings are for the purpose of illustrating the invention only and not for the purpose of limiting the same, and in which there is shown by way of illustration only and not in the drawings in which there is no intention to limit the invention thereto; to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if there is an orientation or positional relationship indicated by terms such as "upper", "lower", "left", "right", "front", "rear", etc., based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not an indication or suggestion that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes, and are not to be construed as limiting the present invention, and the specific meaning of the terms may be understood by those skilled in the art according to specific situations.
Referring to fig. 1-6, the reference numbers in the figures refer to the following elements: the device comprises a guide rail system 10, a belt wheel 11, a conveyor belt 12, a slider connecting plate 13, a guide rail slider 14, a motor mounting plate 15, a first driving motor 16, a photoelectric switch 17, a guide rail 18, a scanning detection system 20, a second driving motor 21, a photoelectric chute 22, a photoelectric slider 23, an emission light filter 24, a reflection light filter 25, a stroke detection switch 26, a scanning detection connecting plate 27, a photoelectric conversion sensor 28, an excitation light source 29, a signal acquisition and conversion system 30, a reagent card placing system 40, a reagent card placing plate 41, a reagent card slot blocking plate 42, a display lamp 43, a spring plate 44, a reagent card 50 and a mounting base plate 60.
The invention relates to a multi-channel fluorescence immunoassay analyzer, which comprises a reagent card placing system 40, a scanning detection system 20, a signal acquisition and conversion system 30 and a guide rail system 10; the reagent card placement system 40 is adapted to removably mount a plurality of reagent cards 50; the scanning detection system 20 is used for generating emission light, irradiating the emission light on the reagent card 50 and collecting the reflection light of the reagent card 50; the signal acquisition and conversion system 30 is used for converting the reflected light signal acquired by the scanning detection system 20 into an electrical signal and detecting the fluorescence intensity of the reagent card 50 through photoelectric signal processing; the scanning detection system 20 is mounted on the rail system 10, and the rail system 10 is used for conveying the scanning detection system 20 along the extending direction of the reagent card placing system 40, so that the scanning detection system 20 sequentially passes through the reagent cards 50 mounted in the reagent card placing system 40.
Optionally, the guide rail system 10 extends in a straight line and includes two belt wheels 11, a conveyor belt 12 and a first driving motor 16, the scanning detection system 20 is mounted on the conveyor belt 12, the number of the belt wheels 11 is two, and the conveyor belt 12 is sequentially arranged around the two belt wheels 11; the first driving motor 16 is used for driving one of the belt pulleys 11 to rotate, so as to drive the scanning detection system 20 to move; the extending direction of the reagent card placing system 40 is also a straight line, and the extending direction of the reagent card placing system 40 is parallel to the extending direction of the guide rail system 10; the first driving motor 16 is arranged on the motor mounting plate 15; the guide rail system 10 is also provided with a photoelectric switch 17 for detecting the position of the scanning detection system 20; the guide rail system 10 further comprises a guide rail 18 for guiding the traveling direction of the scanning detection system 20, the scanning detection system 20 is mounted on the guide rail 18, and the extension direction of the guide rail 18 is parallel to the extension direction of the conveyor belt 12; the guide rail 18 is also provided with a guide rail sliding block 14 and a sliding block connecting plate 13 fixedly connected with the guide rail sliding block 14, and the scanning detection system 20 is connected with the sliding block through the sliding block connecting plate 13 and is arranged on the guide rail 18; a stroke detection switch 26 for detecting the position of the rail block 14 is also included.
In this embodiment, a stepping motor is used as the first driving motor 16, and the first driving motor 16 drives the conveyor belt 12 to move, so that the scanning detection system 20 can scan and detect a plurality of reagent cards 50 within a stroke range, and the second driving motor 21 drives the photoelectric slider 23 to scan on a single reagent card 50. In different embodiments, the guide rail system 10 can be implemented by other forms such as a screw slider.
Optionally, the scanning detection system 20 includes a second driving motor 21, a photoelectric chute 22, a photoelectric slider 23, a photoelectric conversion sensor 28, and an excitation light source 29; the photoelectric slide block 23 is arranged inside the photoelectric chute 22 and driven by the second driving motor 21 to move in a direction close to or far away from the reagent card 50; the excitation light source 29 is mounted on the photoelectric slider 23 and generates emission light; the photoelectric conversion sensor 28 is mounted on the photoelectric slider 23 and collects reflected light; the photoelectric slide block 23 is further provided with an emission light filter 24 and a reflection light filter 25, and emission light generated by the excitation light source 29 is irradiated on the reagent card 50 through the emission light filter 24; the reflected light from the reagent card 50 is transmitted through the reflected light filter 25 and is irradiated on the photoelectric conversion sensor 28; the device also comprises a scanning detection connecting plate 27, and the excitation light source 29, the photoelectric conversion sensor 28, the emission light filter 24 and the reflection light filter 25 are all connected with the photoelectric slide block 23 through the scanning detection connecting plate 27.
In this embodiment, an optical lens group is further provided for distinguishing the emitted light from the reflected light; the emission light filter 24 is a narrow-band filter, which only allows light signals with emission light wavelength to pass through, and can reduce the excitation and interference of stray light on the fluorescence of other substances; the fluorescent substance generates a reflected light of a fluorescent signal after being excited by the emitted light; a narrow-band filter for the wavelength of the reflected light is also installed, and is a reflective light filter 25, which functions to allow only the optical signal of the wavelength of the reflected light to pass through, and can shield the emitted light and the other stray optical signals from the excitation of other possible substances.
With the movement of the scanning detection system 20, the light generated by the excitation light source 29 forms a movement similar to scanning in the area of the reagent card 50, and during the whole movement, the scanning detection system 20 collects the reflected light signal at any time and transmits the reflected light signal to the signal collection and conversion system 30. The signal collection and conversion system 30 converts the optical signal into an electrical signal, and determines the concentration of the reactant according to the intensity of the reflected light. The structure of the light path of the emitted light and the light path of the reflected light in this embodiment form a certain included angle.
Optionally, the reagent card placing system 40 includes a plurality of reagent card slots arranged in parallel; a spring piece 44 for clamping the reagent card 50 and a reagent card slot blocking piece 42 for limiting the position of the reagent card 50 in the reagent card slot are arranged in each reagent card slot; and each reagent card slot is also provided with a display lamp 43 for detecting whether a reagent card 50 is installed in the reagent card slot. A plurality of parallel reagent card slots are all arranged on the reagent card 50 placing plate 41; the reagent card placing system 40, the scanning detection system 20, the signal acquisition and conversion system 30, and the guide rail system 10 are all disposed on the mounting base plate 60.
The invention integrates the analysis of the problems in the prior art, adopts a linear multi-channel scheme, can meet the requirement of multi-channel detection, simplifies the structure and ensures the accuracy and reliability of the detection.
Finally, the above embodiments are only intended to illustrate the technical solutions of the present invention and not to limit the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the claims of the present invention.
Claims (9)
1. A multi-channel fluorescence immunoassay analyzer is characterized in that: the system comprises a reagent card placing system, a scanning detection system, a signal acquisition and conversion system and a guide rail system; the reagent card placement system is used for detachably mounting a plurality of reagent cards; the scanning detection system is used for generating emission light, irradiating the emission light on the reagent card and collecting reflected light of the reagent card; the signal acquisition and conversion system is used for converting the reflected light signals acquired by the scanning detection system into electric signals and detecting the fluorescence intensity of the reagent card through photoelectric signal processing; the scanning detection system is arranged on the guide rail system, and the guide rail system is used for conveying the scanning detection system along the extension direction of the reagent card placing system so that the scanning detection system sequentially passes through the reagent cards arranged in the reagent card placing system; the extension direction of the guide rail system is a straight line and comprises belt wheels, two conveying belts and a first driving motor, wherein the scanning detection system is arranged on the conveying belts, and the conveying belts sequentially pass around the two belt wheels; the first driving motor is used for driving one belt wheel to rotate so as to drive the scanning detection system to move forwards; the extending direction of the reagent card placing system is also a straight line, and the extending direction of the reagent card placing system is parallel to the extending direction of the guide rail system; the reagent card placing system comprises a plurality of reagent card slots arranged in parallel.
2. The multi-channel fluoroimmunoassay analyzer of claim 1, wherein: the guide rail system further comprises a guide rail used for guiding the traveling direction of the scanning detection system, the scanning detection system is installed on the guide rail, and the extending direction of the guide rail is parallel to the extending direction of the conveyor belt.
3. The multi-channel fluoroimmunoassay analyzer of claim 2, wherein: the scanning detection system is connected with the sliding block through the sliding block connecting plate and is arranged on the guide rail.
4. The multi-channel fluoroimmunoassay analyzer of claim 1, wherein: the scanning detection system comprises a second driving motor, a photoelectric sliding groove, a photoelectric sliding block, a photoelectric conversion sensor and an excitation light source; the photoelectric sliding block is arranged in the photoelectric sliding groove and is driven by a second driving motor to move along the direction close to or far away from the reagent card; the excitation light source is arranged on the photoelectric slide block and generates emission light; the photoelectric conversion sensor is mounted on the photoelectric slider and collects reflected light.
5. The multi-channel fluoroimmunoassay analyzer of claim 4, wherein: the photoelectric sliding block is also provided with an emission light filter and a reflection light filter, and emission light generated by the excitation light source penetrates through the emission light filter and irradiates on the reagent card; the reflected light from the reagent card is transmitted through the reflected light filter and is applied to the photoelectric conversion sensor.
6. The multi-channel fluoroimmunoassay analyzer of claim 5, wherein: the photoelectric slide block is characterized by further comprising a scanning detection connecting plate, and the excitation light source, the photoelectric conversion sensor, the emission light filter and the reflection light filter are connected with the photoelectric slide block through the scanning detection connecting plate.
7. The multi-channel fluoroimmunoassay analyzer of claim 1, wherein: each reagent clamping groove is internally provided with a spring piece for clamping the reagent card and a reagent clamping groove blocking piece for limiting the position of the reagent card in the reagent clamping groove.
8. The multi-channel fluoroimmunoassay analyzer of claim 7, wherein: and each reagent clamping groove is also provided with a display lamp for detecting whether a reagent card is installed in the reagent clamping groove.
9. The multi-channel fluoroimmunoassay analyzer of claim 7, wherein: a plurality of parallel reagent clamping grooves are arranged on the reagent card placing plate.
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| CN201811643387.7A CN109521197B (en) | 2018-12-29 | 2018-12-29 | Multi-channel fluorescence immunoassay analyzer |
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| CN111077306B (en) * | 2019-12-30 | 2023-08-25 | 长沙协大生物科技有限公司 | Quantitative detection analyzer for fluorescent immunoreagent and detection method thereof |
| CN111624331A (en) * | 2020-05-22 | 2020-09-04 | 军事科学院系统工程研究院卫勤保障技术研究所 | Multichannel synchronous fluorescence immunochromatography detection system |
| CN112816719B (en) * | 2020-12-25 | 2023-12-12 | 重庆康巨全弘生物科技有限公司 | POCT full-automatic fluorescence immunoassay appearance |
| CN112748245B (en) * | 2020-12-30 | 2024-07-19 | 浙江省智能诊疗设备制造业创新中心 | Multichannel fluorescence immunochromatography quantitative detector and detection method thereof |
| CN113533211B (en) * | 2021-08-20 | 2024-05-07 | 四川新健康成生物股份有限公司 | Bar code recognition device and algorithm for fluorescence analyzer |
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| CN106706937A (en) * | 2017-02-05 | 2017-05-24 | 深圳市活水床旁诊断仪器有限公司 | Quasi-automatic immunity analyzer and detecting method |
| CN206756837U (en) * | 2017-05-21 | 2017-12-15 | 苏州和迈精密仪器有限公司 | A kind of scanning means of multi-linked immunity chromatography reagent card |
| CN207164074U (en) * | 2017-08-02 | 2018-03-30 | 南京岚煜生物科技有限公司 | A kind of multichannel Immunofluorescence test device |
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| WO2017117011A1 (en) * | 2015-12-31 | 2017-07-06 | Gen-Probe Incorporated | Systems and methods for analyzing a sample and for monitoring the performance of an optical signal detector |
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| CN106706937A (en) * | 2017-02-05 | 2017-05-24 | 深圳市活水床旁诊断仪器有限公司 | Quasi-automatic immunity analyzer and detecting method |
| CN206756837U (en) * | 2017-05-21 | 2017-12-15 | 苏州和迈精密仪器有限公司 | A kind of scanning means of multi-linked immunity chromatography reagent card |
| CN207164074U (en) * | 2017-08-02 | 2018-03-30 | 南京岚煜生物科技有限公司 | A kind of multichannel Immunofluorescence test device |
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